LOCUS MN582080 4298 bp DNA circular ENV 18-NOV-2019 DEFINITION Microviridae sp. ctjyu33, complete genome. ACCESSION MN582080 VERSION MN582080.1 DBLINK BioProject: PRJNA540674 BioSample: SAMN11552893, SAMN11552892, SAMN11552894 Sequence Read Archive: SRR8996939, SRR8996941, SRR8996944 KEYWORDS ENV. SOURCE Microviridae sp. ctjyu33 (freshwater metagenome) ORGANISM Microviridae sp. ctjyu33 Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae. REFERENCE 1 (bases 1 to 4298) AUTHORS Malki,K. and Breitbart,M. TITLE Florida's Freshwater Springs JOURNAL Unpublished REFERENCE 2 (bases 1 to 4298) AUTHORS Malki,K. and Breitbart,M. TITLE Direct Submission JOURNAL Submitted (16-OCT-2019) College of Marine Science, University of South Florida, 830 1st St S, Saint Petersburg, FL 33701, USA COMMENT Submissions were annotated with Cenote-Taker2 available at https://github.com/mtisza1/Cenote-Taker2. ##Genome-Assembly-Data-START## Assembly Method :: SPAdes v. 3.12 Genome Coverage :: 11.517683x Sequencing Technology :: Illumina ##Genome-Assembly-Data-END## FEATURES Location/Qualifiers source 1..4298 /organism="Microviridae sp. ctjyu33" /mol_type="genomic DNA" /isolate="ctjyu33" /isolation_source="Volusia Spring" /db_xref="taxon:2656703" /environmental_sample /country="USA" /collection_date="22-May-2017" /metagenome_source="freshwater metagenome" /note="genome binned from sequencing reads available in SRX5775735,SRX5775734,SRX5775731; metagenomic" CDS 1..468 /inference="similar to AA sequence:UniProtKB:A0A0C5AFV1_9VIRU" /codon_start=1 /transl_table=11 /product="putative minor capsid protein" /protein_id="QGH72842.1" /translation="MSFPGEGLISGTIAGGLNYLGQREANKMNLRIAREQMGFQERMS STAYQRAMQDMAQAGLNPILAAGGGASSPSGASATMMNEMSGAVSSAIQAKAVTAQIQ QTKAMTDLLKADLPGHEAEAKVYGSKFGVLLKVLQALSSPVNSAAGLLRAFGK" CDS 476..886 /inference="protein motif:PFAM:PF09675.9" /codon_start=1 /transl_table=11 /product="scaffold protein" /protein_id="QGH72843.1" /translation="MSLVQRLEVDFKNSPSLTRQEFKKECDINQIMKKFKKVMGVDYL SKFNSLTGGQFGDFSEVSDYQTALNVVKQSEAMFMALPAIVRKRFDNDTAKFLDFCHD PKNADEMVTLGLREKPVAVAKPVEAAKPPVPAAG" CDS complement(953..1105) /note="No HHsearch hits" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="QGH72849.1" /translation="MIKKILQSKHIKLFYQNRLLTHPTFRRTTLEPTKRELPFQTHTN TTPINS" CDS complement(1014..1217) /note="No HHsearch hits" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="QGH72848.1" /translation="MSHVRIQRMYPIRNMTKMKTSRFVAHCLSLVHTKTSFHDKKNPS IQTYQAVLPKPASDAPDISSNNT" CDS 1333..2247 /inference="protein motif:PFAM:PF05840.12" /codon_start=1 /transl_table=11 /product="replication associated protein" /protein_id="QGH72844.1" /translation="MPCYHPLQAYFSVRSDGKKDVKFSNALAKNFAAGIAMPEDSLQV PCGRCMGCRLERSRQWAVRMMHEAKLYEDNCFVTLTFDEKNLFEKCSNGSLDKKHVQD FMKALRYRFSDRKIRFFACGEYGDALFRPHYHLCLFNVDFADKVKWKKVNGFWYFNSD LLASCWTFGHSVISDLTFESAGYVARYCTKKVTGSVAKEHYGDRLPEFAQMSLKPGLG RGWLDRWGKTDCFAHDDVVVRGVQCKPPRYYDKVLESLDSARFELVKESRKARAQEKA GDSTFSRLAVREKCLEARIKNLIRRMEK" CDS 2251..2517 /inference="similar to AA sequence:UniProtKB:A0A0G2UEC9_9VIRU" /note="DNA binding protein VP5" /codon_start=1 /transl_table=11 /product="DNA binding protein" /protein_id="QGH72845.1" /translation="MILKVFTVYDSKVEAFLPPFFMKSKGEAVRGFTEVCNDSKSNFF KYPEDYTLFEVGSFDDATGKFDLHKTLVSIGLGVEFKKVADAKA" CDS complement(2397..2588) /note="No HHsearch hits" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="QGH72847.1" /translation="MPGDAPRHPHLKLGRLPSPRPQASYAFASATFLNSTPSPIETRV LCKSNFPVASSKEPTSNKV" CDS 2677..4290 /inference="protein motif:PFAM:PF02305.16" /codon_start=1 /transl_table=11 /product="major capsid protein" /protein_id="QGH72846.1" /translation="MLRGSVPSIQSSAQHTFARAPQAGIPRSKFNRSHGVKTTFDEGY LVPVLVDEMLPGDTFSLKMHAFARMATPIYPIMDNLYLESFFFSVPYRLVWTHWEQFN GANIPDTNSTTDYTIPQCSAPAMGGVATGDLQDYMGIPIKVNSLTFNNLHMRAYNLIW NDWFRDQNLQNQVTVDTGDGPDTYTNYVLLQRGKRHDYFTSCLPFPQKGPDVEIPLGT SAPVYADALATPGQSRFLIGGVGTPYTSNGTIYTQATTGITLGSDNSTTIIYDPENSL YADLSTATAATINALRLAWQTQKLYERDARGGTRYTEIIKSHFGVISPDMRLQRPEYL GGGSTPVVINPVAQTAQTTSPTNTDVLGNLAAYGVCAPQGHGFTYSATEHCLIIGLVS VRADINYQQGLERMWSRQTRLDYYWPALSHIGEQVVLNQEIYAQGNSDDTEVFGYQER FAEYRYKPSQITGLFRSTATSPLDAWHLAQKFTSLPTLGSTFIVENAPMSRIEAVSSQ PDFLFDCFFSYHCARPMPVYAIPGLVDHF" ORIGIN 1 atgagttttc caggcgaggg tttgatttcc ggcactattg ccggaggttt gaattatctt 61 gggcagcgtg aagcgaacaa gatgaatttg cgtatagcta gggagcagat gggttttcaa 121 gagcggatga gctcgacggc gtatcagaga gccatgcaag atatggctca agccggcttg 181 aatcccattt tagccgctgg tgggggcgct agctctccta gcggcgcttc tgcgactatg 241 atgaatgaga tgagcggagc ggttagctcc gctatacaag ccaaggctgt tactgctcag 301 attcagcaga ctaaggctat gactgatctt ttgaaagctg atcttccagg ccatgaggcc 361 gaggcgaagg tttatggttc taagttcggt gttttattga aggttttgca agctttgtca 421 tcgccggtta attcggctgc tggtttgctt agagcatttg ggaagtgagg tttttatgtc 481 tttagttcaa cgtttagagg ttgattttaa gaattctccg tctttgactc gtcaggagtt 541 taagaaggag tgtgatatca atcagattat gaagaagttc aagaaggtga tgggagtcga 601 ttatttgagt aagtttaatt cgcttacagg aggccaattt ggcgacttta gcgaggtatc 661 tgactaccag actgccttga acgtcgttaa gcaatctgag gccatgttta tggccttgcc 721 agcgattgtg cgaaagcggt ttgataatga taccgctaag tttttggatt tttgtcatga 781 tccaaaaaat gctgatgaga tggttactct tggtttgaga gagaaaccag ttgcggttgc 841 aaaaccggta gaggcggcta agccgccagt tcccgccgcc ggttaaggcg gggggtttgt 901 ccctggcaca tgtgtccttc ttgacgtaaa tgtgccaggt gacgaatact gactagctat 961 taataggtgt ggtattcgta tgggtttgaa agggaagttc ccttttagta ggttcaagtg 1021 ttgttcgacg aaatgtcggg tgcgtcagaa gccggttttg gtagaacagc ttgatatgtt 1081 tggattgaag gatttttttt atcatgaaag gaagtttttg tatgtacaag agaaaggcaa 1141 tgagccacaa agcggctcgt cttcatttta gtcatgttgc gaataggata catccgttga 1201 atgcgcacat ggctcatagt tttccgatgc gtggcggttt tcgcctgtaa attctgagcg 1261 tcgttagtgc gacgctcaga ggtgttttgt gttgagatcc cgcaaccgag tcgttacgag 1321 gttatcgagt atatgccttg ttatcatcct ttgcaagctt atttttccgt cagatctgat 1381 ggtaagaaag atgttaagtt ttctaatgcg ttagctaaga attttgctgc tggtattgct 1441 atgcctgaag attctcttca ggttccttgt ggtaggtgta tgggttgtcg gcttgagaga 1501 tctcgtcagt gggcggttcg tatgatgcat gaagcgaagc tttatgagga taattgtttt 1561 gttacattga ctttcgatga gaagaatctg tttgaaaagt gttctaatgg ttctttggat 1621 aagaagcatg ttcaagattt tatgaaggct ttgcgttata ggttttctga taggaagatt 1681 cgtttttttg cttgtggtga gtatggagat gctttatttc gtccacacta tcatttgtgt 1741 ttgtttaatg tggattttgc agacaaggtt aagtggaaga aagttaacgg tttttggtat 1801 tttaattctg atttattggc ttcttgttgg acctttggtc attccgttat ttctgatttg 1861 acgtttgagt ctgctggtta tgtagctcgt tattgtacga agaaggtcac cggttccgtt 1921 gctaaggaac attatggtga ccgtttgcct gagtttgctc agatgagttt aaagcctggc 1981 ctcggtagag gttggcttga tagatggggc aaaactgatt gttttgccca tgatgatgtt 2041 gttgtaagag gcgttcagtg taaaccgccc cgttattatg acaaggtgct tgagtcttta 2101 gattcagctc gttttgagct ggttaaggag agccgtaagg ctcgagctca agagaaggct 2161 ggtgattcga cttttagtcg tttagccgtt agagagaagt gtcttgaggc acgtattaag 2221 aatttaattc gacgaatgga gaagtgattt atgattttaa aggtgtttac tgtttatgat 2281 tctaaggttg aggcgttttt accaccgttt tttatgaagt ccaagggcga agccgttcgt 2341 ggttttactg aggtttgtaa tgattctaag tctaattttt ttaagtatcc tgaagattat 2401 actttgtttg aggttggttc ttttgatgat gctactggaa aatttgattt acacaaaact 2461 cttgtttcga taggacttgg tgtagagttt aagaaggtgg ctgacgcgaa agcgtaagac 2521 gcctggggtc tgggggacgg cagtcgcccc agcttgagat gggggtgcct gggggcatcc 2581 cccggcatta gtttttattt attttggctc gagggtgcgt gaagcgccac attcgcgaag 2641 cggctcaaga gccttttttt taatatagga gtgtttatgt tgagaggttc tgtaccgagt 2701 attcagtcga gtgcacagca tacgtttgct cgtgctcctc aggctggtat tcctcgttcg 2761 aagtttaatc ggtctcacgg agtgaagact accttcgacg agggttattt ggttcctgtt 2821 ttagtagatg agatgttgcc gggagataca ttttctttga agatgcatgc tttcgcacgt 2881 atggctacgc ctatttatcc cattatggac aacctttatt tggagtcttt tttcttttct 2941 gttccttatc gtttggtttg gacacattgg gagcaattta atggtgcgaa tattccggat 3001 accaattcta ctactgatta tactattcct cagtgttccg ctccagctat gggcggtgta 3061 gctactggtg acttgcaaga ttatatgggt attcccatta aggttaatag tttaaccttt 3121 aataacttgc atatgcgtgc ttataattta atttggaatg attggtttag agatcagaat 3181 ttgcagaatc aggttactgt tgatactggt gatgggcctg atacttatac aaattatgtt 3241 cttttgcagc gtggtaagcg tcatgattat tttacatctt gtttgccatt tcctcagaag 3301 ggccctgatg ttgagattcc tttaggtact tctgctcctg tttatgcgga tgctcttgca 3361 actccagggc agtctcgttt tttaattggc ggtgttggta ctccttatac ttctaatggt 3421 actatttata ctcaggctac taccggtata actttgggta gtgataattc tactactatt 3481 atttatgatc ctgagaattc tttatatgct gatttgtcta ctgcgacggc agcgacaatt 3541 aatgctttga ggcttgcttg gcaaactcag aagctttatg agcgggatgc tcgtggaggt 3601 acgcgttata ctgagattat taaatctcat tttggcgtta tatctcctga tatgaggctt 3661 cagaggccag aatatcttgg agggggttct actcctgttg ttattaatcc tgtggctcag 3721 actgcacaga ctacttcgcc tacgaatacg gatgtgttgg gtaatttggc tgcttacggt 3781 gtttgtgctc ctcaaggtca tggttttact tatagcgcta ctgagcattg tttgattatt 3841 ggtcttgttt ctgttcgtgc tgatattaat tatcagcagg ggttagagcg tatgtggagt 3901 cgtcagactc gtcttgatta ttattggcct gctttgtcgc atattggcga gcaagttgtt 3961 ttgaatcagg agatttatgc tcagggcaat tcggatgata cggaagtttt tggttatcaa 4021 gagaggtttg ccgagtatcg gtataaacct tctcagataa ctggtttgtt tagatctacg 4081 gctactagtc cgttggatgc gtggcatttg gcacagaagt ttacttcttt gcctacgctt 4141 ggttctactt ttatagttga gaatgctcct atgagcagga ttgaggctgt tagttctcag 4201 cctgattttc tgtttgattg cttttttagc tatcattgtg caaggccgat gcctgtttat 4261 gctattcctg gacttgttga ccatttttaa ggtgattt //