LOCUS MN582057 4702 bp DNA circular ENV 18-NOV-2019 DEFINITION Microviridae sp. ctudC31, complete genome. ACCESSION MN582057 VERSION MN582057.1 DBLINK BioProject: PRJNA540674 BioSample: SAMN11552893, SAMN11552892, SAMN11552894 Sequence Read Archive: SRR8996939, SRR8996941, SRR8996944 KEYWORDS ENV. SOURCE Microviridae sp. ctudC31 (freshwater metagenome) ORGANISM Microviridae sp. ctudC31 Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae. REFERENCE 1 (bases 1 to 4702) AUTHORS Malki,K. and Breitbart,M. TITLE Florida's Freshwater Springs JOURNAL Unpublished REFERENCE 2 (bases 1 to 4702) AUTHORS Malki,K. and Breitbart,M. TITLE Direct Submission JOURNAL Submitted (16-OCT-2019) College of Marine Science, University of South Florida, 830 1st St S, Saint Petersburg, FL 33701, USA COMMENT Submissions were annotated with Cenote-Taker2 available at https://github.com/mtisza1/Cenote-Taker2. ##Genome-Assembly-Data-START## Assembly Method :: SPAdes v. 3.12 Genome Coverage :: 8.087436x Sequencing Technology :: Illumina ##Genome-Assembly-Data-END## FEATURES Location/Qualifiers source 1..4702 /organism="Microviridae sp. ctudC31" /mol_type="genomic DNA" /isolate="ctudC31" /isolation_source="Volusia Spring" /db_xref="taxon:2656705" /environmental_sample /country="USA" /collection_date="22-May-2017" /metagenome_source="freshwater metagenome" /note="genome binned from sequencing reads available in SRX5775735,SRX5775734,SRX5775731; metagenomic" CDS 1..888 /inference="protein motif:PFAM:PF05840.12" /codon_start=1 /transl_table=11 /product="replication associated protein" /protein_id="QGH72141.1" /translation="MPCYNPVQGYRGRSTNPETGKRPIVFNRKDGYVDLPVTVPCGRC IGCRLERSRMWAVRCLHEASLYPDNCFITLTFSDDHIDKKQTLAVEDFQKFMKRLRKA IHPQTVRFFHCGEYGKNLNHPHHHACLFGWRPPDLELWSERDGVKLYRSKTLEDLWEY GHSTVGDVTFESAAYVARYVTKKITGDPAKKHYGEKKPEYCTMSRRSGIGDAWIKKYK SDVYPDDHVIVNGRPIKVPKFYDAKLELTDPTLYNKIVAIRKDRASKDPNNTLDRLAV REKCKLAKISQLKRGMENE" CDS 881..1228 /inference="protein motif:tr|A0A0G2UEC9|A0A0G2UEC9_9VIRU" /note="DNA binding protein VP5" /codon_start=1 /transl_table=11 /product="DNA binding protein" /protein_id="QGH72142.1" /translation="MNNVICAIYDEKTQVYFGVHESPTIGHAIRQFGDHVKNPNSPLN RHPGDYKLYRIGSMDPTSGLIKQELTPVYLSTALDFLQPITLTPVTTTDEILKSSVEK NVSNTNSRIVNKK" CDS 1252..1728 /inference="protein motif:PFAM:PF09675.9" /codon_start=1 /transl_table=11 /product="scaffold protein" /protein_id="QGH72143.1" /translation="MPKIRNRVKLSFDILDEKGNPHPSVRSRTKQSFADAVNINIIVK KIARTGLAPQKSGAFYGDFSSGDDYQTALQKIQNADEAFMSLPVDIRTRFQNDPSQLL NFLADPKNLEEAVKLGLMDKSLLPATSPLGNGKTKEQIDMEAKQAAAAAAAAASGG" CDS 1945..3531 /inference="protein motif:PFAM:PHA00363" /codon_start=1 /transl_table=11 /product="major capsid protein" /protein_id="QGH72144.1" /translation="MKSGRLPTVMKHNFSQIPRADIPRSVFNRSHGYKTTFDAGYLVP FYLDEALPGDTFKLSATCFARMATPLNPIMDNLYMDIHYFDVPLRLLFRSFQSFMGEQ EPDTFTEYAVPTLPAPTTTGFAVGSLFDYFGVPTEIDDFSISALYSRAYNLIWNEWYR DENIQESVTVDKDDGPDDPADYVLLKRGKRHDYFTSCLPWPQKGDEVDLPLGTVAPIK TGATEDSGEITVQNAAGNYRHLSYNGAAPGTSYVRMSSNAGVEADRLYADLSNATAAT INSLREAFQLQRLLERDARGGTRYVEILRAHFGVISPDARLQRPEYLGGSTAPVAFTP VAQTSKPEATEPLGQLGAVGVITVRGAGFVKSFVEHSIIIGLMSVRADLTYQQGLNRM FSRSTKYDFYWPALAHLGEQAVLNQEIYMQGVPGTGANQDQGVFGYQERWAEYRYKPS QITGKFRSTYATSLDPWHLSQEFSALPTLSPTFIQETPPMARIKAITTEPDFLFDSYI NLTCARPMPVYSVPGLIDHF" CDS 3539..3628 /note="No HHsearch hits" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="QGH72146.1" /translation="MPDYDQLFPHGSSDYDGSWVLIKMPWGCP" CDS 3631..3888 /note="No HHsearch hits" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="QGH72147.1" /translation="MYMLLLIILTVLGCSAISRSIDNYEACVNDPVCYERAKVASQIA AQSAQSAAETSFTIPQMVGTVVGAGAMAITAVWLGRKKRGV" CDS 3888..4565 /inference="protein motif:UniProtKB:A0A0C5AFV1_9VIRU" /codon_start=1 /transl_table=11 /product="putative minor capsid protein" /protein_id="QGH72145.1" /translation="MEHNYDAILNQMPFVGLFLGGILSAVAKPIISVAAKALPFLKNN AGSIINAGGNIAGGLIGAEGAEDANSANAMLARENRDWQERMSNTAHQREVEDLRAAG INPILSAKFGGSSTPPGNVATMTNPGEFTGAGISSAGQAYWTSQLTREQVLTQRSVQS VNSAQAMASRAAALKSLIDAQKIKAEIPAVQAQSERESQGGDIRNSKFFKPFKWFGEI KRQIMGR" ORIGIN 1 atgccatgct ataatcccgt tcagggttat cggggacgct cgactaaccc tgaaacaggc 61 aagcgtccga ttgtctttaa tcgcaaagat gggtacgttg atttacctgt gactgtgcct 121 tgtggtcgtt gtattggttg tcgtcttgaa cgctctcgca tgtgggctgt gcgttgtttg 181 cacgaagcct cactctatcc tgataactgc tttatcactc tcacattctc cgacgatcat 241 attgataaaa aacaaacact tgccgtcgaa gactttcaaa aattcatgaa gcgtttgcgc 301 aaagccattc atccgcaaac ggtacgtttc tttcactgcg gtgaatacgg aaaaaattta 361 aatcatccac atcatcatgc ttgtctcttt ggttggcgtc cacccgatct tgagttatgg 421 tctgaacgtg atggcgtgaa attgtatcgt tcaaaaactc ttgaagatct ttgggagtat 481 ggtcattcta ccgtcggaga tgttacgttt gaatctgcgg cgtatgttgc tcggtatgtg 541 acgaaaaaaa ttactggtga cccggcaaaa aaacattatg gtgaaaaaaa acctgagtac 601 tgtacgatgt ctcgtcgttc gggtattggt gatgcgtgga ttaaaaaata taaatctgat 661 gtgtaccctg acgatcatgt gattgtcaac ggacgcccta taaaagttcc gaagttctac 721 gatgcaaaat tagagttgac ggatccaacg ttgtataata aaatcgtcgc aatccgaaaa 781 gatcgtgctt caaaagatcc gaataatacc ttggaccgat tggcggtccg ggagaaatgc 841 aaactggcta aaattagcca actaaaaagg ggaatggaaa atgaataacg ttatctgtgc 901 aatctacgat gaaaaaactc aagtttattt tggagtgcat gaatctccta ctatcgggca 961 tgcaattcgt caatttggcg atcatgtaaa aaatcctaat tctccactca atcgtcatcc 1021 cggtgattat aaactttacc gaatcgggtc tatggatccg acttccggtc ttattaaaca 1081 ggaacttaca cctgtctatc tttcgactgc gttggatttc cttcaaccta taacattgac 1141 tcctgttact acaactgatg aaatccttaa atcgtccgtc gagaaaaatg tgtctaacac 1201 aaattctcga attgtcaata aaaaataact aaattaaaaa aggacaatac tatgcctaaa 1261 attcgaaatc gtgttaaatt gtctttcgat attctcgacg aaaaaggaaa tcctcatccg 1321 tctgtgcggt cacgcactaa gcagtcgttt gccgacgctg ttaatattaa tataatcgtt 1381 aaaaaaattg ctcgcaccgg acttgcacct caaaaatccg gtgctttcta tggtgatttc 1441 tcgtcgggtg atgactacca aactgcttta caaaaaattc aaaacgccga tgaggcgttt 1501 atgtctttac ctgtcgatat ccgcactcgt ttccaaaacg atccttctca actcttaaac 1561 tttttggctg atcctaaaaa tctcgaggag gccgttaagt taggtcttat ggacaagtcg 1621 cttcttccgg cgacaagtcc acttggtaat ggcaaaacca aggaacaaat tgacatggaa 1681 gctaaacagg ctgcggcggc agccgcagct gccgcatctg gcggctaaat catatatata 1741 tgataagtca gaccattata tacttgatgt aaatggtctg actgacacca aacgtagtga 1801 ggtgtctatg aaaaaagagc tcacagagcg gcaaaaaaaa gttgtaaaag atattgttaa 1861 gtgggttgat gcccagtata aaaaatataa aacacccaag cccggaagcg aacatttccg 1921 ggactatcaa gcagggaggc tctaatgaaa tcaggtcgtt taccgacagt catgaaacac 1981 aacttcagcc agattccacg ggctgatatt ccacgttctg tattcaaccg ttctcatggt 2041 tacaagacta cattcgacgc tggttatctt gtgccgttct atcttgacga ggctcttccc 2101 ggcgacacat tcaaattgag tgcaacgtgt ttcgcacgta tggcgacgcc gcttaatccg 2161 atcatggata atttgtatat ggacatccat tattttgatg ttccgcttcg tcttctcttc 2221 cgatcttttc aatcgttcat gggtgaacaa gagcctgata cgtttaccga atatgctgtt 2281 ccgacgttac cagctccgac aactactggt ttcgccgtcg ggagtttgtt tgattatttc 2341 ggtgtcccga cggaaatcga tgatttttca atttccgctc tttattctcg tgcttataat 2401 ttgatttgga acgaatggta tcgtgacgaa aacattcaag aatctgttac cgtcgataaa 2461 gatgatggtc ccgatgatcc ggctgattat gtgttgttga aacgtggcaa acgtcatgat 2521 tattttacgt cgtgtttgcc gtggccgcaa aaaggtgatg aggttgattt gcctcttggc 2581 accgtcgcac cgattaagac gggtgctact gaagattctg gcgagattac tgttcaaaac 2641 gccgctggta attatcgtca tttaagttat aacggtgccg cacccggcac gtcttatgtg 2701 cgtatgtcgt ctaacgccgg tgtcgaagct gatcgtcttt atgctgattt atctaatgcg 2761 actgcggcga caatcaattc acttcgtgaa gcattccaac ttcaacgctt actcgaacgt 2821 gatgcacggg gcggtaccag atacgttgaa atcttgcgtg ctcacttcgg tgttatttct 2881 cccgatgctc gtctccagcg tcctgaatat ctcggtggtt ctacggctcc tgtggcgttt 2941 acccctgtcg cccagacttc aaaacccgag gccaccgagc ctcttggcca acttggtgcc 3001 gtcggcgtga ttactgtccg tggtgctggt tttgttaaat cgttcgtcga gcattcgatt 3061 attattggtt tgatgtctgt ccgtgctgac ctgacttatc agcaaggttt aaaccgtatg 3121 ttctctcgct cgacaaaata cgatttctac tggcccgctc tggctcatct cggcgagcaa 3181 gccgttttaa accaagagat ttatatgcaa ggcgtacccg gtactggcgc aaaccaagat 3241 caaggcgtgt ttggttatca agaacgttgg gccgagtaca gatacaagcc gtctcaaatt 3301 accggaaaat tccgttcgac gtatgccaca agtctggatc cgtggcattt gtcacaagaa 3361 ttttctgcgc ttcccacatt gtcacccaca ttcattcaag aaacgccgcc gatggcccgt 3421 attaaggcga tcacaactga gccggacttc ttgtttgatt cgtacattaa tttaacctgt 3481 gcccgtccta tgcctgttta cagcgtaccg ggcttgatcg atcattttta agcaaattat 3541 gccagattac gatcaattat ttccccacgg ctcatcggac tatgatgggt cgtgggtctt 3601 aattaaaatg ccgtgggggt gtccatgaaa atgtacatgt tgctgttgat aattctgaca 3661 gttcttggtt gttccgctat ttcaagatca atcgataatt atgaagcttg tgttaatgac 3721 ccggtctgtt atgaacgtgc gaaagtcgct tctcaaattg cggcacaatc cgcacaatcc 3781 gccgctgaaa cgtcgttcac tattccgcaa atggttggga ccgttgttgg tgctggcgcc 3841 atggcgatta ctgctgtctg gcttggtcgt aaaaaaaggg gtgtataatg gaacacaatt 3901 atgatgcgat tttaaatcag atgccgtttg ttggtttatt tcttggcggc atactgtctg 3961 ctgtcgctaa acctattatt tctgtggcgg ccaaggcttt accgttttta aaaaataatg 4021 ctggttcgat tattaacgcc ggtgggaata ttgctggtgg tcttattggt gctgaaggtg 4081 ctgaagatgc gaattctgct aacgctatgt tggctcgtga aaatcgtgat tggcaagagc 4141 gcatgtcaaa cacggctcat caacgtgagg ttgaagatct tcgagccgct ggtattaatc 4201 caattctttc agctaagttt ggcggctctt ctacgccgcc cggcaatgtt gctacgatga 4261 ccaatcccgg tgaatttact ggtgctggta tttcatctgc cggacaagca tattggacga 4321 gtcaattgac tcgtgaacag gttcttactc aacgttctgt acaatccgtt aattctgctc 4381 aagcgatggc gtctcgggcc gccgctttga aatcgcttat tgatgcacaa aaaattaagg 4441 ctgaaattcc tgctgttcag gctcaatcag agcgtgaatc tcaaggtggc gatattcgta 4501 attctaaatt ctttaaaccg ttcaaatggt ttggtgaaat caaacgtcaa attatgggtc 4561 gataatttaa aggaggtgat atcatgagac gaaaaaaaat tcgcaatgtc cgtcgagaca 4621 agcgggtctt taccgctagt gcctataaca ctaatggacg caattttaat gctaatccga 4681 tgagaggcgg ttacagactc ta //