LOCUS LC644557 17405 bp DNA linear PHG 08-JUL-2022 DEFINITION Tetragenococcus phage phiWJ7 DNA, complete genome. ACCESSION LC644557 VERSION LC644557.1 DBLINK BioProject: PRJDB12096 BioSample: SAMD00394880 Sequence Read Archive: DRR311536 KEYWORDS . SOURCE Tetragenococcus phage phiWJ7 ORGANISM Tetragenococcus phage phiWJ7 Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Rountreeviridae. REFERENCE 1 AUTHORS Wakinaka,T., Matsutani,M., Watanabe,J., Mogi,Y., Tokuoka,M. and Ohnishi,A. TITLE Ribitol-Containing Wall Teichoic Acid of Tetragenococcus halophilus Is Targeted by Bacteriophage phiWJ7 as a Binding Receptor JOURNAL Microbiol Spectr 10 (2), e0033622 (2022) PUBMED 35311554 REMARK DOI:10.1128/spectrum.00336-22 REFERENCE 2 (bases 1 to 17405) AUTHORS Wakinaka,T. and Mogi,Y. TITLE Direct Submission JOURNAL Submitted (21-JUL-2021) Contact:Takura Wakinaka Yamasa Corporation, Manufacturing Division; 2-10-1, Araoi-cho, Choshi, Chiba 288-0056, Japan URL :https://www.yamasa.com/ COMMENT ##Genome-Assembly-Data-START## Assembly Method :: SPAdes v. 3.13.0 Genome Coverage :: 5998x Sequencing Technology :: Illumina Miseq ##Genome-Assembly-Data-END## FEATURES Location/Qualifiers source 1..17405 /organism="Tetragenococcus phage phiWJ7" /mol_type="genomic DNA" /strain="phiWJ7" /isolation_source="soy sauce mash" /host="Tetragenococcus halophilus WJ7" /db_xref="taxon:2862503" /country="Japan" /collection_date="2018" repeat_region 1..110 /rpt_type=long_terminal_repeat CDS 122..415 /note="ORF1" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="BCZ76229.1" /translation="MDKINMNDIRGFINMAWDYLLVEDEELILYDDGMFAIRKVGNLS LFEEDIIYVLCLDPTCWIDSDFVKVDNGNVKKDNKKYSDFVKTMSTAILDVIL" CDS 558..782 /note="ORF2" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="BCZ76230.1" /translation="MKFPCKVTKVKRIYYTLDQELHTQIARFERRVSKVEAREILENL GIEYDEILNIKPEYDLEFDIKLDDLEKYIK" CDS 795..1319 /note="ORF3" /codon_start=1 /transl_table=11 /product="ssDNA-binding protein" /protein_id="BCZ76231.1" /translation="MNNENNITANEVMTGSNSLEQVVQETPNFTILQLPNGKYKKMMK YEKFFSKVPETEEEQLTLFKVFNADQDSGLVTSLSDVVGKDFNIEQVYFNPYQSFDEE NGTFSNGVTTTMLSTDGEFFATSSKSVYYTLHNIFEAFGYPNTENYKPVKVEVTSKKM TRGNQINLDLKGRE" CDS 1374..1862 /note="ORF4" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="BCZ76232.1" /translation="MARRRRRRTRDNEEETRRSNERALNGFGHELNIRTTNPDQVERF IESFKRFDQSFGQYAENYNTTWTISDLDLRSMINGKYHQIMKDVLDLEDEKKVVSDVQ QFAIAVSTIYQRSGNSIKRFFNRMRDLVEKTKEFKNELRADNNENNLWNAVKYYVTQH QS" CDS 1873..3120 /note="ORF5" /codon_start=1 /transl_table=11 /product="DNA packaging protein" /protein_id="BCZ76233.1" /translation="MAKFDTSNKEVPEEKQPKELQKFVSTYRKKYIPDKYNQIYMIDE LMNPDIDHYISISNRSDGKSFNYIGFFCQFSYVYGVGFTLIGRHYTVRQAYARFIEKI FDTLKCFDSKDLTMRRTDFYIMVLYKGKCLGVITDLNEATDLKYHSNFLKDYPLIIYD EFLALDDDYESDEWGKLKTIYSSINRDEKIPFIQFPKVIYLGNAVNFSSPILAYLKVY NILENHPINSMKAYGNIVLEMRRNENINQERNLRAFDEDQDNLTLGQFEINNHLIAQD KDYKELSQNPLLFNIKDGAQYIKVRANPYTRLVILSIEKEAEFSVDYVTKLKDKREDN VYLEENFYKDMTKKFDEGLFLFENNFSKDFILQNLYDLDFRKVIKHYQMKDNEPFEEK EKIFQDNYMEDTKKALVNRFFGG" CDS 3124..5427 /note="ORF6" /codon_start=1 /transl_table=11 /product="DNA polymerase" /protein_id="BCZ76234.1" /translation="MHTNKLQKFIKKNKKEKELNLFVDIETLGYNKEAGREKPTEYKN MVYSVAVSFFDEDQLKVEVFPNFNYFFESIFDGLRNSTGIIFNKPPKVKLVYHNGNKY DNHFMLWDILYYYPFVKRKNLFLKNTEHNEDVDAEKIKGLNKEEKQGIILESHIKSAN NLEMNFFIYGVQFETVDNFVKTSVSLETLGEKLYNINVLSEDDLKTSFDYVKYDKDED MTNQGAKQYCLSVFNHLNEAEMTYIINDVVILGYGVKYYSTIFYGFDYSKMTFTANIL DYYNQNRLTSYQLLHKFPEGKKTTHVKYTDYQFTGLNFYDYLKPFYRGGLNFYNQFLR SNILTNAFSMDLNSSYPYVMYNEKIPTYLSDYEDFGEESQKIAWNRSKDFFYLYRMTK WDFNNLVLDKVDSIVLRQMIVKYYTTNEYVNINDYTLRIIEKVTGKKIDYIRCLSYVG FATQRFGSREQLAHNYKIKSEGKKKQEMIMHDPYTYEFTENENQNPFSAEEVNNSKVL LNGLYGIPALRPYYHLFRLNPDHSFYSIPQGFKNSERNIVFSIFVTAVAFYNLIMPLS YLTQDEIDQYFIYCDTDSLYLKKEVYEKIPAEIFDPIKLGAWKIEHEDIKKMYVLNHK KYVYYAEDQIQVACGGIPQDTFDTNMTFENFIQTQFSAGVEITNQRSVFNNQGTISIY SSKTLLDEGRNYYSYFSLDEDEKKQEIIEDIKKTYYDQGLEETLYFETSVGTLSIDDV FRQPEPVKGKNDLFLLKIVENHIKNEI" CDS 5482..5736 /note="ORF7" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="BCZ76235.1" /translation="MEFKNKEELFDFMQQLSDNYESLSKELQEVKESSNQEEQQQNQD ENEQNQDPENGGTPEDATGGGSPDKEHSDEEMDEIQDFLN" CDS 5755..5922 /note="ORF8" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="BCZ76236.1" /translation="MNNENNEMRDMMKTSYDDVKLDKSGELNASMSIQETSQDQYESK IHNVGGSEDGE" CDS 5912..7075 /note="ORF9" /codon_start=1 /transl_table=11 /product="phage capsid protein" /protein_id="BCZ76237.1" /translation="MANKADNVTKATVKALDMWNKANGTSWTFGTNWTNVNTKFETFV NKFLFPKINETNLSNVDLGNRFDWLAKEVDFIGQYSEDYVVLDTVPTTLDLSKPAELM LKRNYPKIATKLYGAGVVRKQKFTLNNNDTRLNFSTLGDGVAYAVGVYKKKISDINVF EEKEIRAMLVDYALNVTQDTREVNSEDDLTNKLYGAILNIQNNSDKYNETNLASGGAI GRYTTQTKLKDVAILTTDTVKTYLLNTVVGNTFQIAGLDPTSRIISFDDLGGVYRVND DVTLNSDQTIDYFRTFGDYQVQKGDVVPAESVLTFDVSGLTEFKDKATEIKPDSDLFA FVFDVNKLKYRRNTKGMLKQPFYNGEFDEVTHWLHYYSFKAISPFFNDVRIGG" CDS 7078..8091 /note="ORF10" /codon_start=1 /transl_table=11 /product="upper collar protein" /protein_id="BCZ76238.1" /translation="MEQPGLKVDLKEDLQQKVIGHRNNFVHIMTSRYYELLPQLIDYR NKEHIAVDFLKLEVGLRNGYEMVIGEATNGKIVLMGYVTNPNTITNAKTIFQQTRQLQ KQDIKFIIPDFLIPAKMREITYLDDCQTGNFVVMRNKVLNYTNDYQIIDHFVEEMAEI TTSRFSIIMQLKIMTFFISENGDESVNEIVSALYNGSPFAKVDKFFDPEEQMLEYHND NGASNLVELKREYQNKISELNNMIGIQSIGVEKESGVSDTEAQANKGATFSNANVYLS ARQKSLDTLNKRFGLEIEAIYNDQVSSELSQKPDSEGGTQGNEQNSNDNGSDQQRTPE EGQ" CDS 8051..8716 /note="ORF11" /codon_start=1 /transl_table=11 /product="lower collar protein" /protein_id="BCZ76239.1" /translation="MDLINSELQKKGNSEFVKDGKLVFFDEDEQFIQKIMKYDEDVEK IVTDKFFKGFKFENEQTDHDFKKTFVNQFLDREIMYQTVEKFGSQVIRLTLQHERYIE TAYEKYQELLTQESLHEDQQDTESNTTQDSTNTTDNRNLRSTLPQDEINMNIENTEMS YADENSIDKQQQKGNETGNQTGKEQGNSKDNNYNVETLKSLQDIFKDIFEIYDRYCFL HVW" CDS 8727..10649 /note="ORF12" /codon_start=1 /transl_table=11 /product="putative phage structural protein" /protein_id="BCZ76240.1" /translation="MTDNKNKRQYYEPWYNDKTDYNTNAPSYYDFLARYKKQLKTITD TINKLLDRNIETKDTSTIKMLKENDWNTNDIIYLLSKIKFSSENEDFYLKNVLNHANC SSKWTLKNNSKDKKGLFSADYKPVLESIDKEIGVLKNKIDDIDDVFYYQKYSPKFISN LGGIRNAVCQSVNIDDQNLNIYETQSDSQEDEGFFITKLAPNGKFLSFMRLEKAGHGT MIGVESRGIKKEPYIWAYLKQYEKLVRFVYQKNKIPTQNELKNMVDFTPESNKGTYFT PVYDKYNGLLAFRFQSGRVETRKKQDVLNGIDKVVGYVDTDTRQNSSDEPMQGCVTYD RFLYYQSGNSTSNMRIAKYDLLDHKKLYDKVYKNVPFFDGVNNANDGIKEPEGLAYFV NPENCNHELLFVITTGAIGKRFDMLYTISKTSNDYWRSINALGSQQYKITRSDGRALS LPEEWKHLNDIKQPGIYHVTNKEAKTINGWPYPVRKQGFYIEVLPKTQVGSILQRITR ASQTLSQKTFTRYMHYNWKEDRWDIGSWTSGMEKSEYVEHAIPRLFDNNLKNITMPGE YYLTNNAFNTYRDSPTSNRADGGYHLYVTAPDSQNHVMQYLDRNSDDLVELFRRRVDV NSNNASNWILVNSNFD" CDS 10665..11471 /note="ORF13" /codon_start=1 /transl_table=11 /product="BppU family phage baseplate upper protein" /protein_id="BCZ76241.1" /translation="MAKRITRNIRSIKDIEKQPKYTNKQNDLLSDEKDVYVRNKDQYE QITGGVKEVNGETPDENGNVNLQLKDFAGQEKIVRKSQLDESLEAIESGYKNYTDNAV EDMAINDRNLLTGTSAEWVSYSFDGYIGDSFIYTPEQLGLSTGDTLTYSVKYDNTKDT NNDSAVNALVNFYSKDDNIIEGIKGNKILDGEVGLSFVTGNIPEGTAEIRAYKAYKNE NDNKKLVSAKEHKLQLGTRATPWTPAPEDIALKSELEQIKQAIINLGGEI" CDS 11471..11692 /note="ORF14" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="BCZ76242.1" /translation="MFNLSDFIEENLTKSYLTGAFSETQINIFALNYLNRGQIDQEVF DKITKFVEDNEPYPEETEEDLEPPKEENT" CDS 11715..13463 /note="ORF15" /codon_start=1 /transl_table=11 /product="phage tail protein" /protein_id="BCZ76243.1" /translation="MARKLTKFQFFKNVPLNDFQNTIHFKSNAERDDYFLNGRHFPSI TFDKEFNWVRDHSEVQLPTSYESMDGVNYCTFLDGFSNQRFYAFVLEKEYINDRVTKM YLLIDVLMTYTQGDVIKQNAEKVFINRMHYSQYDYKKHLHYLRNNDDVLKTTTKKYIQ SNSKIFDDFWVLMLASVNLQKDFGDEDDPKMSTSSGTVYDNIPSPLNIYIVSLDDFPK FMRSLSSYPWIAQNINKNFVLPKDIVTDRYFNKVNTKGDNPPTVYQITGKGKSFDVTI DSMELDFDKLCNIFDLDPDTEGHLLRNEYTTTELYSYDGQRIMIDNGLINQDTSVKNK LHVMTNAGYHNEFAFYYTNYMRQTNEHHDTGVKDGGMFLNNAIVWDTFDEIPMLIDNY QLGLAQNAHQRELAEDKLLSSRIEGVTDEDASPRSRLMDGISLLSNANPVNFMGKITD EYEFYRTQKAQQKDLAISSPTITSQSDANSFQIANDFYGITKKHAFLDSQELQRVKRY YNGFGFQVEDFGQPEIETMTVCNYVQFSGNWVLPDIDPNLMSILKSQLENGVRFWHND ESKDPLTQNLLKNKWR" CDS 13467..14978 /note="ORF16" /codon_start=1 /transl_table=11 /product="peptidase" /protein_id="BCZ76244.1" /translation="MATIKEFAKKKINNDFDLSVDDVAKRCYDTGRFQAYGLSNAQVK KVLNAVKANGISPAFFAAYEASEGYNSSWGWLNHTTPQGNYLQDAKSVSNWLKNQSND MNGKPAWIDYANYKDFVPASVKRKGNKDFKNMPKGSIGRAYIPGTAATTWEVYYPKGL KASYNGVQNYGAPLNDAMQTIEYWGGNIDSSGGSGGDGGGDDGGSGGSGGDDDSDGGS GGGGETITVRPDLSSVNDFFKKFIDDLIKEINKELKKDMNLKGLSKNLYSSKYLIIQK QLDNTYKPKPGKAFTDMLNKAKKDGSKDLKNLVSKLEKDNTYTKETGGDSGGGDSGGG DSGGGDSGKGWYFPYKTDVLTVTSPQGNRWGSYHHGADVVAGSGGGIYAMHSGTVKIS AESVSGWEVSGAMICIQNDKDDGYGGLFVQYEEFAPNSRRVNVGDKVKGGQRIATSGI SGNSTGEHLHFSTNKKGKLVPARRGNWETALDCINLPNKAGKYKLPSGIKKPG" CDS 14991..15434 /note="ORF17" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="BCZ76245.1" /translation="MEFFNKINIYLSFLGLSTGYLFKKSYDFIIKKIQQKKQKENYQE KFINSTIKKEEKQDKQIKNIQDHTQQLSNAQSDIIRRQRVILYNQIRVKSKNLINHGY VTLSDLDALNLLYKEYKDLGGNGTAKILYEQAIKLPKRSDKEDVQ" CDS 15424..15657 /note="ORF18" /codon_start=1 /transl_table=11 /product="phage holin" /protein_id="BCZ76246.1" /translation="MFNEKTYKILKWCILIVIPAFATLVGTIGSTLNLENTDTIVTII IAIDTFLGTSLGIDNKRFYKKQLEKEKEKEESK" CDS 15654..16595 /note="ORF19" /codon_start=1 /transl_table=11 /product="glycosyl hydrolase family 25" /protein_id="BCZ76247.1" /translation="MTLHISDLSNYQKPNIDSYPSDGYIFKATEGTSFVDKNCDIFVQ QAIKAGKPFGIYHFMNGSHWKSQADHFIQSTKNYHKKGILALDYEMYGMQGASVAEKW LDYVKEKTGVAPLIYMNVSTMNGDNWGSVAKKYGLWIAYPNGKGNYPNISYWNTATLH QYTFDPHDQNEFFGDRGTWDKLAGGKPSKQSTQKKPKQIKEDGKAGTGWATRLQEIYN CKIVDGVISRQIKTKSNKYVYSAEWGKGGSNVIKKIQKELKKQGVYSGKIDGNLGPKC VDGMQRIAGTTHDGFISPVSDMIRWTQIQMNKDKRPF" CDS complement(16630..16812) /note="ORF20" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="BCZ76248.1" /translation="MKSLDIKIVRDLNTGKVNFKTKKEITLLAKDLYKAVTKKDVMLD YQTSVKLLNKFNIKVH" CDS complement(16890..17285) /note="ORF21" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="BCZ76249.1" /translation="MKKIFVLKVEQQLESLRKILEDELIESEDELMNDIMDQVKRYNY DVATKNLQQLDDCMDLINQIEGMSEYFSEYYDLLHLSSNEEDIIEDSQYISISNVNDD IEDTLFDIYVNLNESFEKINDIFVEKEVL" repeat_region 17294..17403 /rpt_type=long_terminal_repeat ORIGIN 1 gtgctgtttt gtgcgtggtt cacaatcccc cctcctgtaa atttttactg caaaaatcct 61 tgacttatcc tgaaaatttt gttatactaa ttatagatca gatgaaagga ggtataataa 121 aatggacaaa ataaatatga atgacattcg gggttttata aatatggcat gggattattt 181 gttagtggaa gatgaagaac taattttata tgatgacggt atgtttgcca ttaggaaagt 241 tggtaattta tcattattcg aagaagatat aatttatgtt ttgtgccttg atccaacttg 301 ctggatcgat agtgactttg ttaaagtaga caatggaaat gtgaagaaag ataacaaaaa 361 atatagtgac tttgttaaaa caatgtcaac agcaatatta gatgttatct tataattcaa 421 gcaaaaaatt actttacatc ttagtaaatt aatgttataa taagtcatgt gataaataat 481 aaaaaaatta actatgtggt ccatccgata tattgtaatt tatcacaaaa acattgaaga 541 taaaggaggt aaagaaattg aagtttcctt gtaaagttac taaggttaaa cgtatatatt 601 atacgttaga ccaagagttg cacacccaaa tcgcacgttt tgaaaggcgt gttagtaagg 661 tggaagcaag agaaatactt gagaatttag gcattgaata tgatgaaatt ttaaatatta 721 aacctgaata tgatttagaa tttgatatta agttagatga tttagaaaaa tatattaaat 781 aaggagtttt tataatgaat aatgaaaaca atataacagc aaatgaagta atgacagggt 841 caaacagttt agaacaggtt gttcaagaaa cgccaaactt tacaatttta caactgccta 901 acggaaagta taagaaaatg atgaagtatg aaaagttttt ctcaaaagta ccggaaacag 961 aagaagagca attgacttta ttcaaagtct ttaatgctga tcaagatagt gggcttgtaa 1021 cctcattatc tgatgttgtg ggaaaagatt ttaatattga acaagtatat ttcaatcctt 1081 atcaaagttt tgatgaagaa aacggcacat tttcaaatgg tgtaacaacc actatgcttt 1141 caactgatgg tgagtttttt gccacttctt caaaatcagt ttattatacg ttgcacaata 1201 tttttgaagc atttggttac ccaaacactg aaaattataa acctgttaaa gtagaagtca 1261 caagcaagaa aatgacacgt gggaatcaaa tcaatttaga tttgaaggga agagaataaa 1321 aataagatgg gcgcttaata taagcgtcca ttttttaaag aaagttggtt tatatggcaa 1381 ggcgtagaag aagaagaaca agagataatg aagaagaaac ccgtaggagc aatgaacgtg 1441 ctttaaacgg atttggtcat gaattaaata taagaacaac gaaccctgat caagtggaac 1501 gttttataga aagttttaaa cggtttgatc agtcctttgg tcagtatgct gaaaattata 1561 acaccacttg gacaatctca gatttggatt taagatctat gattaatggt aaatatcatc 1621 aaatcatgaa agatgtatta gatcttgaag atgagaaaaa ggttgtatct gatgtgcaac 1681 aatttgctat tgctgtttca acaatttatc aaagaagtgg gaatagtatt aaacgctttt 1741 ttaatcgtat gcgggattta gtggaaaaga cgaaggaatt taagaatgaa ttgcgtgcgg 1801 ataacaatga aaataattta tggaacgctg ttaaatatta tgttacacaa catcaaagtt 1861 agggggtaaa tcatggcaaa gtttgataca tctaataaag aagtaccaga agaaaaacaa 1921 ccaaaagagc tacaaaaatt tgtatcgact tatcgaaaaa aatatatccc tgataaatat 1981 aatcagatct atatgattga tgagctgatg aatccggata tcgatcacta tatatcgata 2041 tcaaatcgta gtgatggcaa aagttttaac tatatcgggt ttttttgcca attttcttat 2101 gtctatggtg taggctttac tttaattggt cgacactata cggtacggca agcttatgca 2161 agatttattg aaaagatttt tgatacattg aaatgctttg acagtaaaga tttaaccatg 2221 agaagaacag atttttatat catggtgctt tataaaggga agtgtttagg tgtgatcacg 2281 gatctgaatg aagcaacgga cttaaaatat cattcaaatt ttttaaagga ttatccttta 2341 atcatttatg atgaattttt agctttagat gatgattatg aaagtgatga atggggcaag 2401 cttaaaacca tttattcatc aattaatcgt gatgaaaaaa tcccttttat tcaatttcca 2461 aaagtcattt atttagggaa tgcggtcaac ttttctagcc caatcttagc ttatttaaaa 2521 gtatataata tcttagaaaa tcacccaatc aatagtatga aagcgtacgg caatatcgtt 2581 ttagaaatgc gtaggaatga aaatattaat caagaaagaa atttgcgggc gtttgatgaa 2641 gatcaggaca acttaacgct tgggcaattt gaaattaata accatcttat tgcacaagat 2701 aaagattata aagagctttc gcaaaatcct ttactattta atattaaaga tggcgcgcaa 2761 tatattaagg ttagggcaaa tccttatacc cgtttagtca ttttatctat tgaaaaagaa 2821 gcagaatttt cggtggatta tgtaacgaag ttaaaagata agcgggaaga taatgtttat 2881 ttagaagaaa atttttataa agatatgaca aagaaatttg atgaaggttt atttttgttt 2941 gaaaacaatt tttctaaaga ttttatttta caaaatctat acgacttaga ttttcgtaaa 3001 gtgatcaaac attatcaaat gaaagacaat gaaccttttg aagaaaagga aaagatcttc 3061 caagataact atatggaaga tacaaagaaa gcactggtta atcgattttt tggggggtag 3121 tccatgcata caaacaaatt acagaaattt attaaaaaga acaaaaaaga aaaagagtta 3181 aatttgtttg tagatatcga aactttgggc tataacaaag aagccggaag agaaaaaccc 3241 accgagtata aaaatatggt gtacagtgta gcagtatctt tttttgatga agatcagtta 3301 aaagtggagg tttttcctaa ttttaattac ttttttgaat cgatttttga cggtttaaga 3361 aatagtacgg ggattatctt taataaaccc cctaaagtga aattggttta tcataacggg 3421 aataaatatg ataatcactt tatgttatgg gatatcttat actactatcc ttttgttaaa 3481 cgtaaaaacc tatttttaaa aaatacggaa cataatgaag atgtagacgc agaaaaaatt 3541 aaaggtttaa ataaagaaga aaagcaaggg atcattttag aaagtcacat taagtcagcc 3601 aataacttag aaatgaattt ttttatttac ggggtacaat ttgaaaccgt tgataatttt 3661 gtaaaaacaa gcgtaagttt agaaacgcta ggggaaaaat tatataatat taatgtttta 3721 agtgaagatg atctaaaaac tagttttgat tatgtgaaat atgataaaga tgaagatatg 3781 acaaatcaag gcgcaaagca gtattgtttg agtgtgttta atcacttgaa tgaagcagaa 3841 atgacctata ttatcaatga tgttgtgatt ttagggtatg gcgtaaaata ttatagtacc 3901 attttttatg gatttgatta ttcgaaaatg acgtttaccg ctaatatctt agattattat 3961 aaccaaaatc gactgacttc ttaccaattg ttgcataagt ttccagaagg aaaaaagacc 4021 acacacgtaa aatatacgga ttatcagttt acggggttaa atttttatga ttatttaaaa 4081 ccattttatc ggggtgggtt gaatttttat aatcagtttt tacgatcaaa tattttaacg 4141 aatgcgttta gcatggactt aaattctagt tatccttatg tgatgtataa tgaaaaaatt 4201 cccacatatc tatccgacta tgaagatttt ggtgaagaat cccaaaaaat tgcgtggaac 4261 agatcgaaag atttttttta tttgtatcgt atgaccaaat gggattttaa caacttggtg 4321 ttagataaag ttgattctat tgttttaaga caaatgattg ttaagtatta tacgacaaac 4381 gagtatgtga atatcaatga ttatacgtta cgaattatcg aaaaagtcac gggaaaaaaa 4441 attgattata tccgttgttt gtcttatgtt ggttttgcaa cgcaaagatt tggaagtcgg 4501 gaacaattag cgcataatta taaaatcaaa tcagaaggca agaaaaaaca agaaatgatt 4561 atgcatgatc cgtacactta tgaatttaca gaaaatgaaa atcaaaatcc gtttagtgct 4621 gaagaagtca acaattcgaa agtattatta aatgggttgt atggtatccc tgctttacgc 4681 ccgtattatc atttgtttag attaaatccg gatcatagtt tttattcgat cccgcaaggg 4741 tttaaaaata gcgaacgtaa cattgtattt agtatctttg ttacggcagt tgctttttat 4801 aatttgatta tgcctttgtc ttatctcacg caagatgaaa ttgatcagta ttttatatac 4861 tgcgatacgg atagcttata tttaaaaaaa gaagtctatg aaaaaatccc agctgaaatt 4921 tttgatccga tcaagttagg tgcatggaaa attgaacatg aagatatcaa aaaaatgtat 4981 gtcttaaatc ataaaaaata tgtctattat gctgaagatc aaattcaagt ggcttgcggg 5041 ggtatacccc aagatacttt tgatacgaat atgacttttg aaaattttat ccaaacgcaa 5101 tttagtgcgg gtgtagaaat tacgaatcaa cgttccgttt ttaataatca gggaacgatt 5161 tcgatttatt caagtaaaac gttattagat gagggcagaa attactattc atatttttct 5221 ttagatgaag atgaaaaaaa gcaggaaatt atagaagata ttaaaaaaac gtattatgat 5281 caggggttag aagaaacctt atattttgaa acgtccgttg gtacgctatc gatagatgat 5341 gtatttcgac aacctgaacc cgtgaaagga aagaatgatt tgtttttact aaaaattgtg 5401 gaaaatcata taaaaaatga aatatagtgt aaaaaaagta tgattttatg ttataataag 5461 gaaaaaggag gcaaaagacg aatggaattt aaaaataaag aagaattgtt tgactttatg 5521 caacagttat cagataacta tgaatctttg tctaaggaat tgcaagaagt taaagaatca 5581 tctaatcaag aggaacagca acaaaatcag gacgaaaacg agcaaaacca agacccagaa 5641 aacgggggta cacctgaaga cgctacgggc gggggtagtc ctgataaaga acattcagat 5701 gaagaaatgg acgaaattca agactttttg aattaataga aaggagcata taaaatgaat 5761 aatgaaaata atgaaatgcg tgatatgatg aaaacatcat atgatgatgt gaagctggat 5821 aaaagcgggg aattgaatgc aagcatgagc attcaagaaa cttcccaaga tcagtacgaa 5881 agtaagattc ataatgtagg agggtcagaa gatggcgaat aaagcagata atgtaacaaa 5941 agcgactgta aaagccttag atatgtggaa taaagccaac ggcacaagct ggacgtttgg 6001 cacaaattgg actaatgtaa atacaaaatt tgaaacgttt gtcaataagt ttttatttcc 6061 aaaaattaac gagaccaatt tatccaatgt tgatttaggc aatcgttttg actggttagc 6121 taaagaggtc gattttatcg ggcagtatag tgaagattat gttgttttgg atactgtacc 6181 cacaactttg gatttatcta aacctgcgga actgatgcta aaacgcaatt accctaaaat 6241 tgcaaccaag ctttatggtg cgggtgtcgt acgtaaacaa aaatttactt taaacaataa 6301 tgatacacgt cttaattttt caacattagg tgacggggtt gcctacgctg ttggggtgta 6361 taaaaagaaa atttccgata ttaatgtctt tgaagaaaaa gaaattagag ctatgttagt 6421 tgattatgca ttaaatgtca cacaagatac acgtgaagta aacagcgaag acgatttgac 6481 caataaattg tacggagcga ttttaaatat tcaaaataac tctgataaat ataacgagac 6541 gaacttggct tcaggtggtg caattgggcg ttatacaacg caaaccaagc taaaagatgt 6601 ggcgattcta acaacagata cggtaaaaac ttatttatta aataccgttg ttggaaatac 6661 tttccaaatt gctgggttag acccgacaag tcgaattatt tcatttgatg atttaggtgg 6721 cgtttaccgt gtgaatgatg atgtaacgtt aaacagtgat caaacaattg actatttccg 6781 aacatttggg gactatcaag tgcaaaaagg tgacgtagtg cctgctgaat ctgtgttaac 6841 atttgatgtt tccggtctaa ctgagtttaa agataaagca acagaaatta aaccggatag 6901 tgacttattt gcctttgttt ttgacgtgaa taaattgaaa tatcgtagga acacgaaagg 6961 tatgctaaaa caaccatttt ataatggtga atttgacgag gttacgcact ggttgcatta 7021 ttacagcttc aaggcaatca gtccattttt caatgatgta cgtataggag ggtaagcatg 7081 gaacaaccgg ggttaaaagt agatctaaaa gaagatttac aacaaaaagt gatcgggcat 7141 agaaataact ttgtccacat tatgacttcc cggtattatg agttgctccc tcaactgata 7201 gactaccgca ataaagaaca tattgcggta gattttttaa aattagaagt tggactacgt 7261 aacggctatg aaatggttat cggggaagca accaacggga aaattgtttt aatgggatat 7321 gtcacaaatc ctaatacgat cacaaatgca aaaacgatat ttcagcaaac aaggcagtta 7381 caaaaacaag atatcaaatt tattatacct gattttttaa taccagctaa aatgcgtgaa 7441 attacgtatt tagacgattg ccaaaccgga aattttgtgg ttatgcgaaa taaagtattg 7501 aattatacga acgattatca gatcattgac cactttgttg aggaaatggc agaaattaca 7561 accagtcgtt tttcgattat tatgcaacta aaaatcatga ccttttttat atcggaaaat 7621 ggggacgaat ccgtgaacga aattgtttct gctttatata atggttcgcc ttttgctaaa 7681 gtcgataaat tttttgaccc tgaagagcaa atgctggagt atcataatga caacggggct 7741 tcaaatcttg tggaattaaa acgtgagtat caaaataaga tcagtgaatt aaacaatatg 7801 atcgggatac aatcgatcgg agtagaaaaa gaaagcgggg tttcggatac ggaagcccaa 7861 gccaataaag gcgcaacgtt ttcgaatgcc aatgtctatt taagtgctag acagaaaagt 7921 ttggatacct taaacaaaag atttggattg gaaatagaag caatttataa cgaccaagtt 7981 tcgagcgagc tatcgcaaaa accggatagt gagggaggta cgcaaggcaa tgagcaaaac 8041 agcaacgata atggatctga tcaacagcga actccagaag aagggcaata gtgaatttgt 8101 caaagatgga aaattggtat tttttgatga agatgagcaa tttattcaaa aaatcatgaa 8161 atatgatgaa gatgtggaaa aaatcgttac tgataagttt tttaaaggat ttaaatttga 8221 aaatgaacaa acggatcatg attttaaaaa aacttttgtc aatcagtttt tagatcgtga 8281 aattatgtat caaaccgttg aaaaatttgg gagccaagtg atacgattaa cattgcaaca 8341 tgaaagatat attgaaacgg cttatgaaaa atatcaagag ttattaacac aagaaagttt 8401 gcatgaagat caacaagata ctgaaagcaa tacaacccaa gattcgacaa ataccacaga 8461 taatagaaat ttaaggtcga ctttacccca agacgaaatc aatatgaata ttgaaaatac 8521 ggaaatgagt tatgcagatg aaaacagtat agacaagcaa cagcaaaaag gaaacgaaac 8581 cggtaatcaa accggaaaag aacaaggaaa ttcaaaagat aataactata atgtagaaac 8641 attaaagagc ttacaagata ttttcaaaga tatctttgaa atttatgatc gttattgttt 8701 cttgcatgta tggtaggagg aaaattatga cggataataa aaataaaagg cagtattatg 8761 aaccatggta taatgataaa accgattata atacaaatgc cccaagttat tatgattttt 8821 tagctaggta taaaaaacaa ctaaaaacca taacagatac aataaataaa ttattagata 8881 gaaacataga aacaaaagat acaagcacta ttaaaatgtt aaaagaaaat gattggaata 8941 cgaatgatat tatttatctt ttatctaaaa taaaattttc ttctgaaaat gaagatttct 9001 atttaaaaaa tgtattaaat catgcaaatt gctcctcgaa gtggacgtta aaaaataaca 9061 gtaaagataa aaaaggttta ttcagtgctg attataaacc ggtattagaa tcgattgata 9121 aagaaatagg ggttttaaaa aataaaattg atgatattga cgatgttttt tattatcaaa 9181 aatattcacc caaattcatt tctaatttgg gaggaataag aaatgcggtg tgccaatcag 9241 ttaatattga tgatcaaaat ttaaatattt atgaaacgca aagcgattca caagaagatg 9301 agggattttt tattacgaaa ttagcaccta atggaaaatt tttatcattt atgagacttg 9361 aaaaagctgg gcatggtact atgataggtg tcgaaagtag aggaataaaa aaagagcctt 9421 acatttgggc ttatttaaaa caatacgaaa aattagtgcg ttttgtttat caaaaaaata 9481 aaattcctac acaaaatgaa ctaaaaaata tggttgattt tacacctgaa tcaaataaag 9541 gaacttattt tacacccgtt tatgataagt ataatggttt attagctttt cgttttcaaa 9601 gcggtcgagt tgaaacaaga aaaaagcaag acgtattaaa tggaatagac aaggttgtag 9661 gttatgttga tacagatact agacaaaatt cttctgatga acctatgcaa gggtgtgtaa 9721 cttatgatcg atttttatat tatcaatccg gcaactcaac ttctaacatg agaatagcga 9781 aatacgattt attagaccat aaaaagttat atgataaagt ttataaaaat gttccttttt 9841 ttgatggtgt taataatgca aatgatggaa ttaaagaacc tgaaggactt gcttatttcg 9901 ttaaccctga aaattgcaat cacgaacttt tatttgtaat aactaccgga gctataggaa 9961 aacgtttcga catgctttac acgattagta aaacatcaaa tgattactgg cgtagtatta 10021 acgcgttagg tagccaacaa tataaaataa ctaggtcaga tggtagggca ttaagcttac 10081 cagaagaatg gaaacattta aatgatataa aacaaccggg gatttatcat gtaactaata 10141 aagaagcaaa aacaattaat ggttggcctt atccagtaag aaagcaagga ttttatattg 10201 aagtattacc aaaaacacaa gtcggatcaa ttttacaaag aataacaaga gcttcccaaa 10261 cactttctca aaaaactttc acaagatata tgcattataa ttggaaagaa gatcgttggg 10321 atattggatc atggactagt ggaatggaaa aaagtgaata tgtagaacat gctatacctc 10381 gtctatttga taataattta aaaaatatta caatgcccgg agagtactat ctaacaaata 10441 atgcatttaa tacctatcgt gattccccaa ctagtaatcg agcagatgga ggctatcatt 10501 tatatgtaac tgctcctgac tctcaaaatc atgtaatgca atatttagat agaaattcag 10561 acgatttagt agaattattt agaagaagag ttgatgttaa tagcaataac gcttcaaatt 10621 ggattttagt aaatagtaat tttgattaat ataggaggga tattatggca aaacgaatta 10681 caagaaacat tcgttcaatc aaagatattg aaaaacaacc caaatacaca aataaacaaa 10741 atgacttact ttcagatgaa aaagatgttt atgttcgtaa caaagatcaa tatgaacaaa 10801 taacaggtgg cgttaaagaa gttaacggtg aaactcctga tgaaaacggt aatgtgaatt 10861 tacaattgaa agattttgca ggacaagaaa aaatcgtaag aaaaagtcaa ttagatgaat 10921 ctttggaagc tattgaatcc ggttataaga attatacaga taacgccgtt gaggatatgg 10981 caatcaatga ccgaaacttg ctaacgggta cgagtgctga gtgggtatcc tattcttttg 11041 atggatatat aggagattct tttatttata ctcccgaaca attagggtta agtacaggag 11101 atacattaac gtactcagtt aaatacgata acacaaaaga tactaataac gattccgccg 11161 ttaatgcttt ggttaatttt tatagtaaag acgataatat aattgaagga ataaaaggta 11221 ataagatttt agatggtgaa gttgggttaa gcttcgtgac tggtaatatt cccgaaggaa 11281 ctgctgaaat acgagcttac aaagcttata aaaacgaaaa tgataacaaa aaactagtaa 11341 gcgcaaaaga acataaacta caacttggaa cgcgtgccac accatggaca cccgcacccg 11401 aagatatcgc actaaaaagc gagttagaac aaatcaagca agcaatcatc aatctaggag 11461 gagaaatcta atgtttaact tatcagactt tatcgaagaa aatttaacaa aaagttactt 11521 aaccggagct ttttcagaaa cgcaaatcaa tatttttgct ttgaattatt taaaccgtgg 11581 gcaaattgat caagaggttt tcgataaaat taccaagttt gtcgaggaca atgaacctta 11641 tcccgaagaa actgaggaag accttgaacc accaaaagaa gaaaacactt aggcttgtaa 11701 ggaggttgaa tgaaatggct agaaaactaa caaaatttca attttttaaa aatgtccctt 11761 taaacgattt tcaaaatacc attcatttca aatccaatgc ggaacgtgac gactactttt 11821 taaacggtcg tcactttcct agcattacat ttgataaaga atttaattgg gtgcgtgatc 11881 atagcgaggt gcaactccct acgtcttatg aatcaatgga cggggtaaac tattgtacat 11941 ttttagacgg gttttccaat caacgttttt atgctttcgt gttggaaaaa gaatacatca 12001 atgaccgtgt cacaaaaatg tacttattaa tcgatgtatt aatgacttac acacaaggtg 12061 acgttatcaa acaaaatgct gaaaaagtat ttatcaatcg catgcattac agtcaatacg 12121 attataagaa acatcttcat tatttacgaa ataacgatga cgttttaaaa acaacaacaa 12181 aaaaatatat tcaatccaat agtaagatct ttgatgattt ttgggtttta atgctagcta 12241 gcgtgaattt gcaaaaagac tttggcgatg aggacgaccc taaaatgtca acgtctagcg 12301 gaacggttta tgataatatc ccaagtcctt taaatattta tattgtgagt ttagatgact 12361 tcccaaaatt tatgcgctcc ttatctagct atccttggat agcacaaaac atcaataaaa 12421 atttcgttct acccaaagat attgtgaccg accgttattt taataaagtc aataccaaag 12481 gcgacaaccc gcctacggtt taccaaatta ccggcaaagg aaaatctttt gatgtcacaa 12541 tcgatagcat ggaacttgat tttgataaac tttgcaacat tttcgattta gatccagata 12601 ccgaggggca tttattaaga aacgagtata caacaaccga actatattct tatgacggac 12661 aaagaataat gatcgataat ggtttgatca atcaagatac ttccgttaaa aataaactcc 12721 atgtcatgac caatgcgggc tatcataacg aatttgcttt ttattatacc aattacatgc 12781 gccaaacaaa cgaacatcat gataccggcg taaaagacgg gggcatgttt ttaaataatg 12841 ccattgtttg ggataccttt gatgaaatcc caatgctcat tgataattac caattaggat 12901 tagcacaaaa cgcccaccaa agagaactag ccgaggacaa gctattatct agccggattg 12961 agggggtaac agatgaagac gccagcccta gaagccgatt gatggacggt atttccttac 13021 tatctaatgc gaatcccgtt aattttatgg ggaaaatcac tgatgaatat gaattttaca 13081 gaacgcaaaa agcccaacaa aaagacttag ccatatcaag cccaaccatt acaagtcaat 13141 ccgacgccaa ttctttccaa atcgcaaacg atttttacgg gataacaaaa aaacatgcgt 13201 ttttagattc ccaagaattg caacgagtga aacgttatta taatggattt ggttttcaag 13261 tcgaagattt tggacaacct gaaattgaaa ccatgaccgt atgcaactat gttcagtttt 13321 cgggaaattg ggtattaccg gatattgatc ctaatttaat gagtatttta aaatcccaac 13381 ttgaaaacgg ggtgcgattc tggcataatg atgagagtaa agacccacta acacaaaact 13441 tactcaaaaa caaatggagg taaagcatgg caacaattaa agaatttgca aagaaaaaaa 13501 ttaataatga ttttgatcta agtgttgatg atgttgccaa gcgttgttac gataccggaa 13561 gatttcaagc ctacgggtta agtaacgcgc aagttaaaaa agtacttaat gcggtaaaag 13621 caaatggtat ttcgcccgct ttttttgctg cctatgaagc tagtgagggt tataacagct 13681 cttgggggtg gctcaaccat accacacccc aagggaatta tttacaagac gcaaaaagtg 13741 tcagcaattg gttaaaaaat caatcaaacg acatgaacgg aaaacctgct tggattgatt 13801 atgcgaacta taaagatttt gttcccgcca gtgtgaagcg aaaaggcaac aaagatttta 13861 aaaacatgcc caaaggaagc ataggaagag cttacatccc tggaacagcg gcaacaacgt 13921 gggaagtcta ttatcccaaa gggctaaaag cttcctataa cggcgtacaa aattacggcg 13981 cgcctctcaa cgacgccatg caaacgattg aatactgggg tggaaatatc gacagctcag 14041 ggggttcagg gggtgacggt ggcggtgatg atggaggatc tggaggttct ggaggggacg 14101 atgattctga cggaggttct ggcgggggtg gtgaaacaat cacggtcaga cccgatctat 14161 cttctgtcaa tgattttttt aagaaattta ttgatgatct aataaaagag atcaataaag 14221 agctaaaaaa agatatgaat ctcaaaggat tatccaaaaa cttatactca agtaaatatt 14281 taattataca aaaacagtta gataacacgt acaaacctaa acccggaaaa gcctttacgg 14341 atatgctcaa taaagccaaa aaagacggtt caaaagattt gaaaaatctt gtcagcaagt 14401 tagaaaaaga taacacctac accaaagaaa ccggagggga ttccggtggc ggtgattccg 14461 gtggcggtga ttccggaggc ggtgattccg gtaaaggttg gtattttcct tataaaactg 14521 acgttttaac ggttaccagt ccgcaaggaa accgttgggg aagttatcac cacggggctg 14581 atgtggtcgc tggctcaggc ggtggaattt atgccatgca ttctggcacc gtgaaaatat 14641 ccgccgaaag tgtttcaggt tgggaagtat ccggtgctat gatatgtata caaaatgaca 14701 aagacgacgg atacggcggg ttgttcgtac aatacgagga atttgcaccc aattcacgcc 14761 gggttaacgt cggggataaa gtcaaaggcg gtcaaagaat cgccacatca ggcattagcg 14821 gaaacagtac aggggaacac ttgcactttt caaccaataa aaaaggcaaa ttagtccccg 14881 cacggcgcgg gaattgggaa accgcgttag actgtatcaa tttacccaat aaagcaggga 14941 aatataaact accttcagga attaaaaaac ccgggtaagg aggcaaaacc gtggaatttt 15001 tcaacaaaat taatatttat ttatcttttt tagggttgtc cacgggatat cttttcaaaa 15061 aaagttatga ttttatcata aaaaagatac aacaaaagaa gcaaaaagaa aattatcaag 15121 aaaaatttat caactccaca ataaaaaaag aagaaaaaca agataaacaa ataaaaaata 15181 ttcaagatca tacacaacaa ctatcaaatg cacaatctga tatcatcaga agacaaagag 15241 tgatcttata caatcaaatt cgtgtcaaat ctaaaaactt gatcaatcat ggttatgtta 15301 cattatcaga tctggacgct ttaaatttac tttataaaga gtataaagac ttaggcggaa 15361 atggtacagc aaagatttta tacgaacaag caataaaatt gcctaaaagg agcgataaag 15421 aagatgttca atgaaaaaac atacaaaatt ttaaaatggt gtattctaat tgtaataccc 15481 gcatttgcta cacttgtagg tacaatagga agtacactaa acttagaaaa taccgatacc 15541 atagtaacca ttatcatagc tattgataca ttcttaggta ctagcctagg tattgacaac 15601 aaacgttttt ataaaaaaca attagaaaaa gaaaaagaaa aagaggaatc aaaatgactt 15661 tacacatttc agatctttcc aattatcaaa aaccaaatat tgattcttat ccctcagacg 15721 gatatatttt taaagcaacc gaaggcacat cttttgttga caagaattgc gatatctttg 15781 tccaacaagc cattaaagca ggaaagccct ttggtatata tcatttcatg aacgggagcc 15841 attggaaatc acaagccgat cattttattc aatcgacaaa aaattatcat aaaaagggga 15901 ttttagccct agattatgaa atgtatggta tgcaaggggc ttcagtagct gaaaagtggc 15961 tagattatgt taaagaaaaa acaggcgttg cacccctaat ttatatgaat gtttccacca 16021 tgaacgggga taattggggg tcagttgcca aaaaatatgg actatggata gcttacccta 16081 atggaaaagg aaactaccca aatatttctt attggaatac tgccacatta catcaataca 16141 cctttgatcc acatgatcaa aacgaattct ttggtgatcg tggaacatgg gataaattag 16201 caggtggcaa gcctagcaaa caatccaccc aaaagaaacc aaaacaaatc aaagaagacg 16261 gaaaagctgg aactggatgg gcaacacgct tgcaagaaat ctacaattgt aaaattgttg 16321 atggtgtcat ttcacggcaa atcaaaacca aaagtaacaa atatgtttat tctgctgaat 16381 ggggcaaagg tggttcaaat gtcatcaaaa aaattcagaa agaattgaaa aaacaaggcg 16441 tttacagtgg taaaattgac ggtaatctag gaccaaaatg tgtagacggt atgcaacgaa 16501 ttgctggcac aacacatgac ggtttcatta gtccagtaag tgatatgatt agatggacac 16561 aaattcaaat gaacaaagat aaacgacctt tttaaataaa gtatccctca tttatttgag 16621 ggatattttt tagtggactt ttatattaaa tttattaagt aactttacag acgtttgata 16681 atctagcata acatcttttt tagttacagc tttataaaga tctttagcta aaagcgttat 16741 ttctttctta gttttaaaat ttactttccc agtatttaag tcacgaacaa tctttatatc 16801 caatgatttc aatcctttct tatataaata gataacccca taataaaaaa agtgctaaca 16861 cttgcaaaaa caataataaa ctaaacatat tataacacct ccttttctac aaatatatca 16921 ttaatttttt caaaagattc atttaaattt acatatatat caaacaacgt atcttcaata 16981 tcatcattca catttgatat agaaatatat tgtgaatctt caattatatc ttcttcatta 17041 gatgataaat gtaatagatc ataatattca ctaaaatatt cactcattcc ctcaatttga 17101 tttattaaat ccatacagtc atctaattgt tgtaaatttt tagttgccac atcatagtta 17161 taccttttta cttgatccat tatgtcgttc attaattcat cttctgattc aattaattca 17221 tcttctaaaa tctttcttag actttccaac tgttgctcaa cttttaatac gaatattttt 17281 ttcatttata tcttcctttc atttgatcta taattagtat aacaaaatta tcaggataag 17341 tcaaggattt ttgcagtaaa aatttatagg aggggggatt gtgaaccacg cacaagacag 17401 cactc //