locus-tag class description Thewi_0001 ENOG4105CI4 it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box) 5'-TTATC CA A CA A-3'. DnaA binds to ATP and to acidic phospholipids (By similarity) Thewi_0002 ENOG4105CZ8 DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped onto DNA, it slides freely (bidirectional and ATP- independent) along duplex DNA (By similarity) Thewi_0003 ENOG410848D S4 domain protein YaaA Thewi_0009 ENOG4108Z7R Chaperone CsaA Thewi_0010 ENOG4105C7D DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings (By similarity) Thewi_0011 ENOG4105C24 DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings (By similarity) Thewi_0012 ENOG4105CCY NADH flavin oxidoreductase, NADH oxidase Thewi_0015 ENOG4105CYQ Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) (By similarity) Thewi_0017 ENOG4105MQS Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate (By similarity) Thewi_0018 ENOG4105C0C Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate (By similarity) Thewi_0019 ENOG4105C01 Dihydroxy-acid dehydratase Thewi_0020 ENOG4105C7K acetolactate synthase Thewi_0021 ENOG4105D1W Converts 2,5-diamino-6-(ribosylamino)-4(3h)-pyrimidinone 5'-phosphate into 5-amino-6-(ribosylamino)-2,4(1h,3h)- pyrimidinedione 5'-phosphate (By similarity) Thewi_0022 ENOG4108R6K riboflavin synthase, subunit alpha Thewi_0023 ENOG4105C66 Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate (By similarity) Thewi_0024 ENOG4108UTT Catalyzes the formation of 6,7-dimethyl-8- ribityllumazine by condensation of 5-amino-6-(D- ribitylamino)uracil with 3,4-dihydroxy-2-butanone 4-phosphate. This is the penultimate step in the biosynthesis of riboflavin (By similarity) Thewi_0025 ENOG4108REF Sporulation integral membrane protein ytvi Thewi_0028 ENOG4105CGR Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec) (By similarity) Thewi_0029 ENOG4108KGH TRANSCRIPTIONal Thewi_0040 ENOG4108JPZ Monovalent cation H antiporter subunit D Thewi_0041 ENOG4108JPZ Monovalent cation H antiporter subunit D Thewi_0042 ENOG4105CMZ NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient. This subunit may bind ubiquinone (By similarity) Thewi_0043 ENOG4105ES3 NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient (By similarity) Thewi_0044 ENOG4105CQV NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient (By similarity) Thewi_0045 ENOG4105CQV NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient (By similarity) Thewi_0046 ENOG4105P3U NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient (By similarity) Thewi_0050 ENOG4105D22 carbon starvation protein Thewi_0051 ENOG4105EKQ RNA-directed DNA polymerase Thewi_0057 ENOG4108Z6B deaminase Thewi_0058 ENOG4105DW3 Response regulator receiver modulated metal dependent phosphohydrolase Thewi_0059 ENOG4105C6E dna polymerase iii Thewi_0060 ENOG410816I Binds to DNA and alters its conformation. May be involved in regulation of gene expression, nucleoid organization and DNA protection (By similarity) Thewi_0061 ENOG4105F3K May play a role in DNA repair. It seems to be involved in an RecBC-independent recombinational process of DNA repair. It may act with RecF and RecO (By similarity) Thewi_0062 ENOG4105WMR Protein CrcB homolog Thewi_0064 ENOG4105DHT Metal Dependent Phosphohydrolase Thewi_0068 ENOG4105CHR reductase Thewi_0070 ENOG4105ETE Transcriptional regulator (LacI family Thewi_0071 ENOG4105XN3 extracellular solute-binding protein family 1 Thewi_0073 ENOG4105DRW binding-protein-dependent transport systems inner membrane Component Thewi_0075 ENOG4105CKB glycosidase Thewi_0076 ENOG4105CKB glycosidase Thewi_0081 ENOG4107EXU Short-chain dehydrogenase reductase Sdr Thewi_0082 ENOG4108IG5 Transcriptional regulator Thewi_0083 ENOG4105ECB domain protein Thewi_0085 ENOG4105CB3 Required for the thiolation of cytidine in position 32 of tRNA, to form 2-thiocytidine (s(2)C32) (By similarity) Thewi_0086 ENOG4107GFK fumarate Thewi_0088 ENOG4108UN4 phage SPO1 DNA polymerase-related protein Thewi_0091 ENOG41084XP SirA-like domain-containing protein Thewi_0092 ENOG4105CUC YeeE YedE family protein Thewi_0096 ENOG4107QN2 dihydrolipoyl dehydrogenase Thewi_0097 ENOG4105KE9 The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein (By similarity) Thewi_0099 ENOG4105DKQ ROK family Thewi_0100 ENOG4105CM0 Catalyzes the synthesis of GMP from XMP (By similarity) Thewi_0102 ENOG4105CXN decarboxylase Thewi_0103 ENOG4108ZMD Phosphorylation of dTMP to form dTDP in both de novo and salvage pathways of dTTP synthesis (By similarity) Thewi_0104 ENOG4105X0R protein from nitrogen regulatory protein P-II Thewi_0106 ENOG4107QRG dna polymerase iii Thewi_0107 ENOG4105MA4 psp1 domain protein Thewi_0110 ENOG4108K9K Uncharacterised protein family (UPF0236) Thewi_0112 ENOG4105CEU Arginine dihydrolase Thewi_0113 ENOG4107S47 Specifically methylates the adenine in position 37 of tRNA(1)(Val) (anticodon cmo5UAC) (By similarity) Thewi_0114 ENOG4105CU0 Catalyzes the 2'-O-methylation of the ribose of cytidine 1402 (C1402) in 16S rRNA (By similarity) Thewi_0116 ENOG4105KRN Transcriptional regulator, abrb family Thewi_0117 ENOG4108JIC nucleoside recognition domain protein Thewi_0119 ENOG4105D4J Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate Thewi_0121 ENOG4105CKH Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation (By similarity) Thewi_0122 ENOG4105F8V Hydrolase, tatD family Thewi_0123 ENOG41069ER domain protein Thewi_0124 ENOG4105D1X Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits (By similarity) Thewi_0128 ENOG4105WJF Thewi_0129 ENOG4108WZW RNA polymerase sigma Thewi_0130 ENOG4105D1F ATP-binding protein Thewi_0133 ENOG4105C3H Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins. Plays a role in the quality control of integral membrane proteins (By similarity) Thewi_0134 ENOG4105D65 subunit l Thewi_0135 ENOG4105CMZ NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient. This subunit may bind ubiquinone (By similarity) Thewi_0136 ENOG4105C47 NADH ubiquinone oxidoreductase 20 kda subunit Thewi_0138 ENOG4108HM5 NADH-ubiquinone oxidoreductase chain 49kDa Thewi_0140 ENOG4105WMM Probably plays a role in a hydrogenase nickel cofactor insertion step (By similarity) Thewi_0144 ENOG4105CQS hydrogenase expression formation protein HypD Thewi_0147 ENOG4105CK6 Two component transcriptional regulator (Winged helix family Thewi_0149 ENOG4105C4P Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template (By similarity) Thewi_0150 ENOG41082DI 50s ribosomal protein l31 Thewi_0151 ENOG4107104 thymidine kinase Thewi_0152 ENOG4105CBJ Metal-dependent enzyme Thewi_0153 ENOG4105EQY Methylates the class 1 translation termination release factors RF1 PrfA and RF2 PrfB on the glutamine residue of the universally conserved GGQ motif (By similarity) Thewi_0155 ENOG41081FK Mediates zinc uptake. May also transport other divalent cations (By similarity) Thewi_0156 ENOG4105EK0 Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine (By similarity) Thewi_0157 ENOG4108YZG isomerase Thewi_0158 ENOG4105CZ5 Catalyzes the conversion of uracil and 5-phospho-alpha- D-ribose 1-diphosphate (PRPP) to UMP and diphosphate (By similarity) Thewi_0160 ENOG4105ESV Glycosyl transferase, family 4 Thewi_0161 ENOG4105EAG UDP-N-acetylglucosamine 2-epimerase Thewi_0164 ENOG4105CDF Cell wall formation. Adds enolpyruvyl to UDP-N- acetylglucosamine (By similarity) Thewi_0167 ENOG4105DCF alanine dehydrogenase Thewi_0172 ENOG4108SHJ s-layer protein Thewi_0176 ENOG4105MT0 Sporulation transcriptional regulator spoiiid Thewi_0178 ENOG4108JQA flagellar Thewi_0179 ENOG4105CGA flagellar basal-body rod protein FlgG Thewi_0181 ENOG4108YXN Involved in unsaturated fatty acids biosynthesis. Catalyzes the dehydration of short chain beta-hydroxyacyl-ACPs and long chain saturated and unsaturated beta-hydroxyacyl-ACPs (By similarity) Thewi_0185 ENOG4105DBW Multi-copper polyphenol oxidoreductase laccase Thewi_0188 ENOG4105C1W two component, sigma54 specific, transcriptional regulator, Fis family Thewi_0189 ENOG4105CTS PTS system mannitol-specific Thewi_0193 ENOG4105CPQ Dehydrogenase Thewi_0196 ENOG41090UW PTS System Thewi_0197 ENOG4105DT3 PTS system, galactitol-specific IIc component Thewi_0198 ENOG4105DG4 malate L-lactate dehydrogenase Thewi_0199 ENOG4105CHR reductase Thewi_0204 ENOG4105CXK mandelate racemase muconate lactonizing Thewi_0206 ENOG4108UHU Aldolase Thewi_0207 ENOG4108SC4 )-transporter Thewi_0209 ENOG4105C1W two component, sigma54 specific, transcriptional regulator, Fis family Thewi_0210 ENOG4108UZY PTS System Thewi_0211 ENOG4105ETZ PTS System Thewi_0212 ENOG4105F3R pts system mannose fructose sorbose family iid Thewi_0213 ENOG4108XCU pts system Thewi_0214 ENOG4105D2N Aldolase Thewi_0216 ENOG4105E70 isomerase Thewi_0219 ENOG4107Z96 xylose isomerase Thewi_0220 ENOG4105C93 Xylose Isomerase Thewi_0221 ENOG4105CMG Carbohydrate kinase Thewi_0224 ENOG4105F66 Binding-protein-dependent transport systems inner membrane component Thewi_0226 ENOG4105CS2 ec 3.2.1.21 Thewi_0228 ENOG4105EP9 oxidoreductase Thewi_0230 ENOG4105D17 glycoside hydrolase, family 3 domain protein Thewi_0232 ENOG4105CE4 GlcNAc 6-P deacetylase Thewi_0234 ENOG4105EPD Oligopeptide transporter, Opt family Thewi_0236 ENOG4105CHM Aminotransferase Thewi_0237 ENOG4108ZB6 transcriptional regulator AsnC family Thewi_0238 ENOG4108K9K Uncharacterised protein family (UPF0236) Thewi_0240 ENOG4105CE1 Ferrous iron transport protein b Thewi_0243 ENOG4108JJB ABC transporter Thewi_0244 ENOG4108YZP cobalt ABC transporter, inner membrane subunit CbiQ Thewi_0245 ENOG4105G01 cobalamin (vitamin B12) biosynthesis CbiM protein Thewi_0246 ENOG4105CFT Part of the energy-coupling factor (ECF) transporter complex CbiMNOQ involved in cobalt import (By similarity) Thewi_0247 ENOG4105MRR Transcriptional regulator (By similarity) Thewi_0248 ENOG4105D9Q decarboxylase (Beta subunit) Thewi_0251 ENOG4105CQ3 Poorly processive error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by polIV. Exhibits no 3-5 exonuclease (proofreading) activity. May be involved in translesional synthesis in conjunction with the beta clamp from polIII (By similarity) Thewi_0252 ENOG4105UQX Mate efflux family protein Thewi_0253 ENOG4105DJY efflux transporter, rnd family, mfp subunit Thewi_0254 ENOG4105D6C Part of the ABC transporter complex MacAB involved in macrolide export. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation (By similarity) Thewi_0255 ENOG4105D6C Part of the ABC transporter complex MacAB involved in macrolide export. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation (By similarity) Thewi_0261 ENOG4105C5N The heterodimer acts as both an ATP-dependent DNA helicase and an ATP-dependent, dual-direction single-stranded exonuclease. Recognizes the chi site generating a DNA molecule suitable for the initiation of homologous recombination Thewi_0262 ENOG4107R2I The heterodimer acts as both an ATP-dependent DNA helicase and an ATP-dependent, dual-direction single-stranded exonuclease. Recognizes the chi site generating a DNA molecule suitable for the initiation of homologous recombination. The AddA nuclease domain is required for chi fragment generation Thewi_0266 ENOG4105D3R alkyl hydroperoxide reductase Thewi_0269 ENOG4108K9K Uncharacterised protein family (UPF0236) Thewi_0271 ENOG41070F3 Catalyzes the ATP-dependent phosphorylation of maltose to maltose 1-phosphate. Is involved in a branched alpha-glucan biosynthetic pathway from trehalose, together with TreS, GlgE and GlgB (By similarity) Thewi_0272 ENOG4107QPH glycogen) synthase Thewi_0273 ENOG4107ZTW SNARE associated Golgi Thewi_0277 ENOG4105C80 Catalyzes the reversible oxidation of malate to oxaloacetate (By similarity) Thewi_0282 ENOG4105EAY pump that utilizes the energy of pyrophosphate hydrolysis as the driving force for Thewi_0283 ENOG4107NFR Fructose-1,6-bisphosphatase Thewi_0285 ENOG4105C2F Histidine kinase Thewi_0286 ENOG4108VB1 regulatoR Thewi_0287 ENOG4105DFW ABC transporter Thewi_0288 ENOG4105DFW ABC transporter Thewi_0289 ENOG4105C2J ABC transporter Thewi_0290 ENOG4108HWW ABC transporter Thewi_0292 ENOG4107QK8 The glycine cleavage system catalyzes the degradation of glycine. The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor Thewi_0295 ENOG4105DF9 Lipoate-protein, ligase Thewi_0296 ENOG4107QN2 dihydrolipoyl dehydrogenase Thewi_0298 ENOG4105CHM Aminotransferase Thewi_0299 ENOG4106ETB IIa component Thewi_0300 ENOG4105F55 Catalyzes the isomerization of sedoheptulose 7-phosphate in D-glycero-D-manno-heptose 7-phosphate (By similarity) Thewi_0303 ENOG4108HHA Transketolase Thewi_0306 ENOG4105CTQ phosphohexokinase Thewi_0309 ENOG4105CHR reductase Thewi_0310 ENOG4105DKT Transcriptional regulator Thewi_0312 ENOG410787A Pts system, glucitol sorbitol-specific Thewi_0313 ENOG4105DZY Pts system, glucitol sorbitol-specific Thewi_0316 ENOG4105VI0 Pts system, glucitol sorbitol-specific Thewi_0317 ENOG4105CW3 Transaldolase is important for the balance of metabolites in the pentose-phosphate pathway (By similarity) Thewi_0325 ENOG4108K9K Uncharacterised protein family (UPF0236) Thewi_0328 ENOG4107QW4 alcohol dehydrogenase Thewi_0342 ENOG4105MKU Transcriptional regulator Thewi_0345 ENOG4105CIZ coA-substrate-specific enzyme activase Thewi_0346 ENOG4105PGM cation diffusion facilitator family transporter Thewi_0348 ENOG4108T5I Part of the ABC transporter complex PstSACB involved in phosphate import. Responsible for energy coupling to the transport system (By similarity) Thewi_0349 ENOG4105C4B ABC transporter, permease Thewi_0350 ENOG4105C8T Leucyl-tRNA synthetase Thewi_0351 ENOG4108UMN azlc family Thewi_0353 ENOG4105DD7 kinase (PfkB family Thewi_0354 ENOG4105D53 Catalyzes the hydrolysis of pseudouridine 5'-phosphate (PsiMP) to ribose 5-phosphate and uracil (By similarity) Thewi_0356 ENOG41089WN small, acid-soluble spore protein tlp Thewi_0357 ENOG4105CJ3 phosphate transporter Thewi_0361 ENOG4105C1W two component, sigma54 specific, transcriptional regulator, Fis family Thewi_0364 ENOG4105CK1 PTS System Thewi_0366 ENOG4105F8D glycoside hydrolase family 4 Thewi_0369 ENOG4105C46 Catalyzes the first step in hexosamine metabolism, converting fructose-6P into glucosamine-6P using glutamine as a nitrogen source (By similarity) Thewi_0370 ENOG4105CDP Catalyzes the condensation of (S)-aspartate-beta- semialdehyde (S)-ASA and pyruvate to 4-hydroxy- tetrahydrodipicolinate (HTPA) (By similarity) Thewi_0371 ENOG4108JQ1 Dehydrogenase Thewi_0372 ENOG4105DG4 malate L-lactate dehydrogenase Thewi_0373 ENOG4105VCP PTS System Thewi_0374 ENOG4105QAX Iron-sulfur Thewi_0375 ENOG4105WE2 aldehyde ferredoxin oxidoreductase Thewi_0379 ENOG4105D06 uba thif-type nad fad binding protein Thewi_0385 ENOG4108RIR Molybdenum cofactor biosynthesis protein Thewi_0386 ENOG4105CVM Molybdenum cofactor synthesis domain protein Thewi_0387 ENOG4105HD0 Transcriptional regulator of molybdate metabolism, LysR family Thewi_0393 ENOG4105CTS PTS system mannitol-specific Thewi_0394 ENOG4105EMD TRANSCRIPTIONal Thewi_0397 ENOG4105CGH 5'-nucleotidase Thewi_0398 ENOG4105CEH Catalyzes the synthesis of dihydrouridine a modified base found in the D-loop of most tRNAs (By similarity) Thewi_0399 ENOG4105EJ9 Membrane Thewi_0401 ENOG4105ETE Transcriptional regulator (LacI family Thewi_0402 ENOG4105ETE Transcriptional regulator (LacI family Thewi_0404 ENOG4105CJM Binding-protein-dependent transport systems, inner membrane component Thewi_0405 ENOG4105C2T Binding-protein-dependent transport systems, inner membrane component Thewi_0408 ENOG4105CNT hydrolase family 2 Thewi_0409 ENOG4105CS2 ec 3.2.1.21 Thewi_0411 ENOG4105FDK ABC transporter Thewi_0412 ENOG4105DSE periplasmic solute binding protein Thewi_0413 ENOG4105N80 Ferric uptake regulator, Fur family Thewi_0414 ENOG4105C7R Atpase associated with various cellular activities aaa_3 Thewi_0417 ENOG4108V0P LemA family Thewi_0418 ENOG4105DUM transcriptional regulator, lysR family Thewi_0419 ENOG4105D5B Sulfite reductase, subunit A Thewi_0420 ENOG4107SHJ sulfite reductase subunit b Thewi_0421 ENOG410810Y Sulfite reductase Thewi_0422 ENOG4105EYA deoxyribonuclease Thewi_0425 ENOG4107YH3 SUF system FeS assembly protein, NifU family Thewi_0428 ENOG4105C4H Specifically methylates the guanine in position 2445 (m2G2445) and the guanine in position 2069 (m7G2069) of 23S rRNA (By similarity) Thewi_0429 ENOG4105JKQ Part of the ABC transporter complex BtuCDF involved in vitamin B12 import. Binds vitamin B12 and delivers it to the periplasmic surface of BtuC (By similarity) Thewi_0430 ENOG4105EHG Permease protein Thewi_0431 ENOG4105E0A ABC, transporter Thewi_0432 ENOG4105C0Y Responsible for the amidation of carboxylic groups at position A and C of either cobyrinic acid or hydrogenobrynic acid. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation (By similarity) Thewi_0437 ENOG4105CIH Imidazole acetol-phosphate transaminase Thewi_0438 ENOG4108YZT Adenosylcobinamide kinase Thewi_0439 ENOG4107XPA Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin) (By similarity) Thewi_0445 ENOG4105C7E Catalyzes the NADPH-dependent reduction of glutamyl- tRNA(Glu) to glutamate 1-semialdehyde (GSA) (By similarity) Thewi_0446 ENOG4105D6W Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps (By similarity) Thewi_0448 ENOG4105D52 delta-aminolevulinic acid dehydratase Thewi_0449 ENOG4105CB8 Multifunctional enzyme that catalyzes the SAM-dependent methylation of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 and then position C-12 or C-18 to form trimethylpyrrocorphin 2. It also catalyzes the conversion of precorrin-2 into siroheme. This reaction consists of the NAD- dependent oxidation of precorrin-2 into sirohydrochlorin and its subsequent ferrochelation into siroheme (By similarity) Thewi_0452 ENOG4105CDM Glutamate-1-semialdehyde aminotransferase Thewi_0458 ENOG4107QX8 SPFH domain, Band 7 family protein Thewi_0492 ENOG4105CN9 Peptidase m42 family protein Thewi_0493 ENOG4105CN9 Peptidase m42 family protein Thewi_0494 ENOG4105CN9 Peptidase m42 family protein Thewi_0495 ENOG4105EKQ RNA-directed DNA polymerase Thewi_0502 ENOG4105DW3 Response regulator receiver modulated metal dependent phosphohydrolase Thewi_0505 ENOG41080BE Methyl-accepting chemotaxis sensory transducer Thewi_0506 ENOG4105CGP Urocanate hydratase Thewi_0507 ENOG4105CCS Glutamate formiminotransferase Thewi_0508 ENOG4105CI2 imidazolone-5-propionate hydrolase Thewi_0514 ENOG4107T3P ABC, transporter Thewi_0515 ENOG4105C2F Histidine kinase Thewi_0516 ENOG4105EQD regulatoR Thewi_0517 ENOG410908R Methenyltetrahydrofolate cyclohydrolase Thewi_0518 ENOG4107TFB basic membrane Thewi_0519 ENOG4108JQ9 ABC transporter Thewi_0520 ENOG4105UIB (ABC) transporter Thewi_0522 ENOG4105FDP GntR family transcriptional regulator Thewi_0524 ENOG4105CZG Phosphotransfer between the C1 and C5 carbon atoms of pentose (By similarity) Thewi_0528 ENOG4105CI1 PTS System Thewi_0529 ENOG4105C5Y PTS System Thewi_0531 ENOG4107SDT Homocitrate synthase Thewi_0539 ENOG4105CBX hi0933 family Thewi_0540 ENOG4108Z2X Catalyzes the pyruvoyl-dependent decarboxylation of aspartate to produce beta-alanine (By similarity) Thewi_0542 ENOG4105CCG Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate (By similarity) Thewi_0544 ENOG41066UU tRNA (Adenine-N1-)-methyltransferase Thewi_0546 ENOG4105H67 Protein of unknown function (DUF421) Thewi_0547 ENOG410667J hydrolase Thewi_0549 ENOG4105ETE Transcriptional regulator (LacI family Thewi_0550 ENOG4105CK6 Two component transcriptional regulator (Winged helix family Thewi_0551 ENOG4105BZU Histidine kinase Thewi_0556 ENOG4108RAC Rubrerythrin Thewi_0557 ENOG4105CPH Catalyzes the formation of S-adenosylmethionine from methionine and ATP Thewi_0558 ENOG4105C7H Helicase, RecD TraA family Thewi_0559 ENOG4105MJ2 Competence protein Thewi_0563 ENOG4105E1Q flagellar hook-associated protein Thewi_0564 ENOG4105DTJ flagellar hook-associated protein Thewi_0566 ENOG4105W5T Binds to the C-terminal region of flagellin, which is implicated in polymerization, and participates in the assembly of the flagellum (By similarity) Thewi_0567 ENOG4105VM8 Could accelerate the degradation of some genes transcripts potentially through selective RNA binding (By similarity) Thewi_0568 ENOG4105I03 Flagellin Thewi_0578 ENOG4107TVC Glycosyl transferase, family 2 Thewi_0579 ENOG4105CAQ asparagine synthetase Thewi_0582 ENOG4107RT6 flagellar hook-associated Thewi_0585 ENOG4107WTJ Methyl-accepting chemotaxis sensory transducer Thewi_0587 ENOG4108HW3 (ABC) transporter Thewi_0588 ENOG4105E5Q amino acid AbC transporter Thewi_0592 ENOG4105DW3 Response regulator receiver modulated metal dependent phosphohydrolase Thewi_0594 ENOG4108Z3G 3H domain protein Thewi_0596 ENOG4105CI6 Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. Has a central role in coupling the hydrolysis of ATP to the transfer of proteins into and across the cell membrane, serving Thewi_0601 ENOG4105CD3 dihydroorotase Thewi_0602 ENOG4105CEU Arginine dihydrolase Thewi_0603 ENOG4105DBV ornithine carbamoyltransferase Thewi_0604 ENOG4105C5B carbamate kinase Thewi_0606 ENOG4105K8N protein family UPF0079, ATPase Thewi_0607 ENOG41072GX Peptidase M22 Glycoprotease Thewi_0609 ENOG4105CPM Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine (By similarity) Thewi_0612 ENOG4105E14 MotA TolQ exbB proton channel Thewi_0613 ENOG4106VK6 Flagellar motor protein Thewi_0615 ENOG4105E39 Enoyl-CoA hydratase Thewi_0616 ENOG4105C1G acyl-Coa dehydrogenase Thewi_0617 ENOG4105BZJ Electron transfer flavoprotein Thewi_0618 ENOG4105C10 Electron transfer flavoprotein Thewi_0620 ENOG4105DYT 3-hydroxyacyl-CoA dehydrogenase Thewi_0621 ENOG4108ZEF Reversibly transfers an adenylyl group from ATP to 4'- phosphopantetheine, yielding dephospho-CoA (dPCoA) and pyrophosphate (By similarity) Thewi_0622 ENOG4105CHU acetyl-coa acetyltransferase Thewi_0624 ENOG4108Z7G MaoC domain protein dehydratase Thewi_0626 ENOG4105CF8 branched-chain amino acid ABC transporter, permease Thewi_0627 ENOG4105C0Z ABC transporter Thewi_0628 ENOG4108IV0 ABC, transporter Thewi_0630 ENOG41074FZ May increase the rate of spontaneous hydrolysis of aminoacrylate to malonic semialdehyde. Required to remove a toxic intermediate produce in the pyrimidine nitrogen degradation (By similarity) Thewi_0631 ENOG4105CCZ Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Catalyzes the first condensation reaction which initiates fatty acid synthesis and may therefore play a role in governing the total rate of fatty acid production. Possesses both acetoacetyl-ACP synthase and acetyl transacylase activities. Its substrate specificity determines the biosynthesis of branched- chain and or straight-chain of fatty acids (By similarity) Thewi_0632 ENOG4105CYC Transferase Thewi_0634 ENOG4105D3C The physiological role of BioH is to remove the methyl group introduced by BioC when the pimeloyl moiety is complete. It allows to synthesize pimeloyl-ACP via the fatty acid synthetic pathway through the hydrolysis of the ester bonds of pimeloyl-ACP esters (By similarity) Thewi_0636 ENOG4105FKZ Methyl-accepting chemotaxis Thewi_0637 ENOG4105CKM Branched-chain carboxylic acid kinase Thewi_0639 ENOG4105DY9 regulatoR Thewi_0640 ENOG4105CEQ signal transduction histidine kinase regulating citrate malate metabolism Thewi_0644 ENOG4105CDJ oxidoreductase FAD NAD(P)-binding domain protein Thewi_0645 ENOG4105BZ8 glutamate synthase Thewi_0646 ENOG4108Z03 MAF-like protein Thewi_0647 ENOG4108UQV Membrane Thewi_0649 ENOG4105RX7 peptidase S8 and S53, subtilisin, kexin, sedolisin Thewi_0665 ENOG4105EKQ RNA-directed DNA polymerase Thewi_0666 ENOG4105CP4 Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate- limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth (By similarity) Thewi_0667 ENOG4105CM0 Catalyzes the synthesis of GMP from XMP (By similarity) Thewi_0668 ENOG4105C7N Xanthine uracil vitamin C permease Thewi_0669 ENOG4108UM6 Catalyzes the conversion of N5-carboxyaminoimidazole ribonucleotide (N5-CAIR) to 4-carboxy-5-aminoimidazole ribonucleotide (CAIR) (By similarity) Thewi_0670 ENOG4105C8V SAICAR synthetase Thewi_0672 ENOG4105D21 Phosphoribosylformylglycinamidine synthase I Thewi_0673 ENOG4107QIK phosphoribosylformylglycinamidine synthase Thewi_0675 ENOG4105CXB phosphoribosylaminoimidazole synthetase Thewi_0676 ENOG4108V3E phosphoribosylglycinamide formyltransferase Thewi_0678 ENOG4107QNG Phosphoribosylglycinamide synthetase Thewi_0679 ENOG410626R Converts the free carboxyl group of a malonyl-thioester to its methyl ester by transfer of a methyl group from S-adenosyl- L-methionine (SAM). It allows to synthesize pimeloyl-ACP via the fatty acid synthetic pathway (By similarity) Thewi_0680 ENOG4105F03 Provides the (R)-glutamate required for cell wall biosynthesis (By similarity) Thewi_0681 ENOG4105CH2 amidohydrolase Thewi_0682 ENOG4105CKP Polysaccharide Biosynthesis Protein Thewi_0684 ENOG4105ETD TRANSCRIPTIONal Thewi_0686 ENOG4105VZJ PTS System Thewi_0687 ENOG4105CK1 PTS System Thewi_0688 ENOG4105VCP PTS System Thewi_0689 ENOG4105KGF protein, YerC YecD Thewi_0690 ENOG4105C4R Atp-dependent dna helicase Thewi_0692 ENOG4105VB5 Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln) (By similarity) Thewi_0694 ENOG4105CHT Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln) (By similarity) Thewi_0696 ENOG4108J59 Extracellular solute-binding protein, family 5 Thewi_0698 ENOG4105CJM Binding-protein-dependent transport systems, inner membrane component Thewi_0699 ENOG4105C2T Binding-protein-dependent transport systems, inner membrane component Thewi_0704 ENOG4105C5C n-acetylmuramoyl-l-alanine amidase Thewi_0705 ENOG4105ED0 Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates (By similarity) Thewi_0710 ENOG4105K7U Cell division inhibitor that blocks the formation of polar Z ring septums. Rapidly oscillates between the poles of the cell to destabilize FtsZ filaments that have formed before they mature into polar Z rings. Prevents FtsZ polymerization (By similarity) Thewi_0711 ENOG4105BZU Histidine kinase Thewi_0714 ENOG4105BZU Histidine kinase Thewi_0715 ENOG4108YZA Nitrogen regulatory protein pii Thewi_0717 ENOG4105EYU Glutamine amido-transferase Thewi_0718 ENOG4105CBC glutamate synthase Thewi_0719 ENOG4105D3S 4Fe-4S Ferredoxin, iron-sulfur binding domain protein Thewi_0725 ENOG4108UV6 Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) (By similarity) Thewi_0726 ENOG4105C4U 4-methyl-5-beta-hydroxyethylthiazole kinase Thewi_0729 ENOG4108UTW Plays a role in the regulation of phosphate uptake Thewi_0731 ENOG4105DCF alanine dehydrogenase Thewi_0732 ENOG4108IV0 ABC, transporter Thewi_0733 ENOG4105C0Z ABC transporter Thewi_0734 ENOG4105CF8 branched-chain amino acid ABC transporter, permease Thewi_0735 ENOG4105C32 Amino acid ABC transporter (Permease) Thewi_0736 ENOG4105CP9 extracellular ligand-binding receptor Thewi_0738 ENOG4108K9K Uncharacterised protein family (UPF0236) Thewi_0739 ENOG4105DEA Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation Thewi_0740 ENOG4105CCQ Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins (By similarity) Thewi_0741 ENOG4105CHN ATP-dependent specificity component of the Clp protease. It directs the protease to specific substrates. Can perform chaperone functions in the absence of ClpP (By similarity) Thewi_0742 ENOG4105C6P ATP-dependent serine protease that mediates the selective degradation of mutant and abnormal proteins as well as certain short-lived regulatory proteins. Required for cellular homeostasis and for survival from DNA damage and developmental changes induced by stress. Degrades polypeptides processively to yield small peptide fragments that are 5 to 10 amino acids long. Binds to DNA in a double-stranded, site-specific manner (By similarity) Thewi_0743 ENOG4105WHM Necessary for normal cell division and for the maintenance of normal septation (By similarity) Thewi_0745 ENOG4107RFS it plays a direct role in the translocation of protons across the membrane (By similarity) Thewi_0746 ENOG41082I9 F(1)F(0) ATP synthase produces ATP from ADP in the presence of a proton or sodium gradient. F-type ATPases consist of two structural domains, F(1) containing the extramembraneous catalytic core and F(0) containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation (By similarity) Thewi_0747 ENOG4107ZFM Component of the F(0) channel, it forms part of the peripheral stalk, linking F(1) to F(0) (By similarity) Thewi_0748 ENOG4107YZM F(1)F(0) ATP synthase produces ATP from ADP in the presence of a proton or sodium gradient. F-type ATPases consist of two structural domains, F(1) containing the extramembraneous catalytic core and F(0) containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation (By similarity) Thewi_0749 ENOG4105CDG Produces ATP from ADP in the presence of a proton gradient across the membrane. The alpha chain is a regulatory subunit (By similarity) Thewi_0750 ENOG4105J80 Produces ATP from ADP in the presence of a proton gradient across the membrane. The gamma chain is believed to be important in regulating ATPase activity and the flow of protons through the CF(0) complex (By similarity) Thewi_0751 ENOG4105C4J Produces ATP from ADP in the presence of a proton gradient across the membrane. The catalytic sites are hosted primarily by the beta subunits (By similarity) Thewi_0752 ENOG4105KNM Produces ATP from ADP in the presence of a proton gradient across the membrane (By similarity) Thewi_0756 ENOG4105EFP binding-protein-dependent transport systems inner membrane Component Thewi_0771 ENOG4105C6Y Udp-glucose 6-dehydrogenase Thewi_0773 ENOG4105E26 Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and or degradation of damaged proteins (By similarity) Thewi_0777 ENOG41068W6 Capsular polysaccharide biosynthesis protein Thewi_0778 ENOG4105H63 Glycosyl transferase, family 2 Thewi_0782 ENOG4107SVN transposase Thewi_0785 ENOG4105C1B dTDP-glucose 4-6-dehydratase Thewi_0787 ENOG4108I19 Catalyzes the formation of dTDP-glucose, from dTTP and glucose 1-phosphate, as well as its pyrophosphorolysis (By similarity) Thewi_0788 ENOG4105DBZ Dtdp-4-dehydrorhamnose reductase Thewi_0789 ENOG4108URP Dtdp-4-dehydrorhamnose 3,5-epimerase Thewi_0806 ENOG4105I03 Flagellin Thewi_0806 ENOG4105I03 Flagellin Thewi_0828 ENOG4108V0P LemA family Thewi_0829 ENOG4108RE9 Phosphodiesterase Thewi_0830 ENOG4107QM2 Aldo Keto reductase Thewi_0837 ENOG41071TK Uncharacterised protein family (UPF0150) Thewi_0846 ENOG4105CY0 Metallo-Beta-Lactamase Thewi_0847 ENOG4105CD9 Involved in the production of pyridoxal phosphate, probably by incorporating ammonia into the pyridine ring (By similarity) Thewi_0848 ENOG4108UHX Involved in the hydrolysis of glutamine to glutamate and ammonia. Channels an ammonia molecule to PdxS (By similarity) Thewi_0849 ENOG4108UYI beta-alanyl-CoA ammonia lyase Thewi_0850 ENOG4105DE9 3-keto-5-aminohexanoate cleavage enzyme Thewi_0851 ENOG4105FRY Dehydrogenase Thewi_0854 ENOG4105C98 muts2 protein Thewi_0855 ENOG4105EQ5 D-Lysine 5,6-aminomutase alpha subunit Thewi_0856 ENOG4107CHF 5,6-aminomutase beta subunit Thewi_0857 ENOG4105CUB amino acid Thewi_0860 ENOG4105C3Y UTP-glucose-1-phosphate uridylyltransferase Thewi_0861 ENOG4105HTX biosynthesis protein Thewi_0862 ENOG4107YJ4 chain length determinant protein Thewi_0864 ENOG4108KGH TRANSCRIPTIONal Thewi_0868 ENOG4108UGX Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate (By similarity) Thewi_0869 ENOG4105C0R drug resistance transporter emrb qaca subfamily Thewi_0872 ENOG4108IFG nitrite reductase, (NAD(P)H) Thewi_0873 ENOG4105D1E Undecaprenyl pyrophosphate phosphatase Thewi_0876 ENOG4108UKJ Cdp-alcohol phosphatidyltransferase Thewi_0877 ENOG4107WTJ Methyl-accepting chemotaxis sensory transducer Thewi_0879 ENOG4105DTC 1-(5-phosphoribosyl)-5-amino-4-imidazole-carboxylate (air) carboxylase Thewi_0882 ENOG4105CK6 Two component transcriptional regulator (Winged helix family Thewi_0887 ENOG4105CA4 amino acids such as threonine, to avoid such errors, it has a posttransfer editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA-dependent manner (By similarity) Thewi_0889 ENOG4105DPM folylpolyglutamate synthase Dihydrofolate synthase Thewi_0895 ENOG4105EH2 Catalyzes the conversion of epoxyqueuosine (oQ) to queuosine (Q), which is a hypermodified base found in the wobble positions of tRNA(Asp), tRNA(Asn), tRNA(His) and tRNA(Tyr) (By similarity) Thewi_0896 ENOG4107KJX Transcriptional regulator Thewi_0898 ENOG4105XN3 extracellular solute-binding protein family 1 Thewi_0899 ENOG4105DB9 Binding-protein-dependent transport systems inner membrane component Thewi_0900 ENOG4105DRS transport system, permease Thewi_0901 ENOG4107URF beta-phospho-glucomutase Thewi_0903 ENOG4105ETE Transcriptional regulator (LacI family Thewi_0905 ENOG4105DRW binding-protein-dependent transport systems inner membrane Component Thewi_0908 ENOG4105RX7 peptidase S8 and S53, subtilisin, kexin, sedolisin Thewi_0909 ENOG4105C1W two component, sigma54 specific, transcriptional regulator, Fis family Thewi_0910 ENOG4105F0V Cell wall hydrolase Thewi_0913 ENOG41068B1 Cob-I-yrinic acid a,c-diamide adenosyltransferase Thewi_0915 ENOG4105DUK Catalyzes the conversion of 4-hydroxy- tetrahydrodipicolinate (HTPA) to tetrahydrodipicolinate (By similarity) Thewi_0916 ENOG4105CDP Catalyzes the condensation of (S)-aspartate-beta- semialdehyde (S)-ASA and pyruvate to 4-hydroxy- tetrahydrodipicolinate (HTPA) (By similarity) Thewi_0917 ENOG4105CM3 Catalyzes the NADPH-dependent formation of L-aspartate- semialdehyde (L-ASA) by the reductive dephosphorylation of L- aspartyl-4-phosphate (By similarity) Thewi_0919 ENOG4105BZ1 (ABC) transporter Thewi_0920 ENOG4105BZ1 (ABC) transporter Thewi_0923 ENOG4105M3Z response regulator Thewi_0924 ENOG4105DYB Isoaspartyl dipeptidase Thewi_0928 ENOG4105DR5 peptidase Thewi_0932 ENOG4105C84 Histidine ammonia-lyase Thewi_0935 ENOG4105CJ1 ABC, transporter Thewi_0937 ENOG4105D07 Signal peptide peptidase, SppA Thewi_0942 ENOG4105CK4 Destroys radicals which are normally produced within the cells and which are toxic to biological systems (By similarity) Thewi_0943 ENOG4108UIC UPF0340 protein Thewi_0951 ENOG41067S9 phage shock protein C, PspC Thewi_0952 ENOG4108ZJ4 polysaccharide deacetylase Thewi_0957 ENOG4105DY9 regulatoR Thewi_0958 ENOG4108HRK Histidine kinase Thewi_0959 ENOG4105EMN RNA polymerase sigma factor Thewi_0962 ENOG4108ZQD Catalyzes the phosphorylation of the 3'-hydroxyl group of dephosphocoenzyme A to form coenzyme A (By similarity) Thewi_0963 ENOG4105E4C Lytic transglycosylase catalytic Thewi_0966 ENOG4107SN7 NLP P60 protein Thewi_0967 ENOG4105CI3 gtp-binding protein typa Thewi_0969 ENOG4105E4C Lytic transglycosylase catalytic Thewi_0973 ENOG4108R3D Electron transport complex Thewi_0976 ENOG410902H NADH dehydrogenase (Ubiquinone), 24 kDa subunit Thewi_0977 ENOG4105BZU Histidine kinase Thewi_0979 ENOG4107QIZ NADH dehydrogenase Thewi_0980 ENOG4107QHI -hydrogenase Thewi_0982 ENOG4108Z03 MAF-like protein Thewi_0983 ENOG4105W5Q DNA repair protein (RadC Thewi_0984 ENOG4105DFF Rod shape-determining protein mreb Thewi_0988 ENOG4107YDG Cell division inhibitor that blocks the formation of polar Z ring septums. Rapidly oscillates between the poles of the cell to destabilize FtsZ filaments that have formed before they mature into polar Z rings. Prevents FtsZ polymerization (By similarity) Thewi_0989 ENOG4107QMS site-determining protein Thewi_0990 ENOG41083CN Prevents the cell division inhibition by proteins MinC and MinD at internal division sites while permitting inhibition at polar sites. This ensures cell division at the proper site by restricting the formation of a division septum at the midpoint of the long axis of the cell (By similarity) Thewi_0991 ENOG4105CNI cell division protein FtsW Thewi_0992 ENOG4108Z5K methylglyoxal synthase Thewi_0993 ENOG4105DR5 peptidase Thewi_0997 ENOG4105C03 ribonuclease Thewi_0998 ENOG4105CSD Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) (By similarity) Thewi_0999 ENOG410807C QueT transporter Thewi_1002 ENOG4105KK9 This protein binds to 23S rRNA in the presence of protein L20 (By similarity) Thewi_1004 ENOG4105K46 50S ribosomal protein l27 Thewi_1007 ENOG4108Z1W Catalyzes the reversible adenylation of nicotinate mononucleotide (NaMN) to nicotinic acid adenine dinucleotide (NaAD) (By similarity) Thewi_1008 ENOG4108WF2 Metal Dependent Phosphohydrolase Thewi_1014 ENOG4105CAQ asparagine synthetase Thewi_1015 ENOG4105DZ1 carboxypeptidase Thewi_1016 ENOG4105KQC Competence protein Thewi_1018 ENOG4108W5N phosphatidylglycerophosphatase a Thewi_1021 ENOG41084EF Ferredoxin Thewi_1022 ENOG4105CM2 brancheD-chain amino acid aminotransferase Thewi_1023 ENOG4105CIM Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction alanine is first activated by ATP to form Ala- AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain (By similarity) Thewi_1026 ENOG4105PUK Membrane Thewi_1029 ENOG4105IES Zinc phosphodiesterase, which displays some tRNA 3'- processing endonuclease activity. Probably involved in tRNA maturation, by removing a 3'-trailer from precursor tRNA (By similarity) Thewi_1030 ENOG4105EGY DNA internalization-related competence protein ComEC Rec2 Thewi_1032 ENOG4105KQV Binds directly to 16S ribosomal RNA (By similarity) Thewi_1034 ENOG4105CEF Initiates the rapid degradation of small, acid-soluble proteins during spore germination (By similarity) Thewi_1040 ENOG4105CSA Coproporphyrinogen iii oxidase Thewi_1041 ENOG4105CI8 Negative regulator of class I heat shock genes (grpE- dnaK-dnaJ and groELS operons). Prevents heat-shock induction of these operons (By similarity) Thewi_1042 ENOG4105K90 Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with DnaK and GrpE. It is the nucleotide exchange factor for DnaK and may function as a thermosensor. Unfolded proteins bind initially to DnaJ Thewi_1043 ENOG4105CFG Acts as a chaperone (By similarity) Thewi_1044 ENOG4105BZ5 ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins (By similarity) Thewi_1045 ENOG4105F59 Methylates ribosomal protein L11 (By similarity) Thewi_1047 ENOG4105CIW Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine (By similarity) Thewi_1048 ENOG4107YU2 histidine triad (hIT) protein Thewi_1049 ENOG4105VCC 30S ribosomal protein S21 Thewi_1051 ENOG4105HDK L-asparaginase Thewi_1052 ENOG41068P3 sporulation protein YqfC Thewi_1054 ENOG4105C9A Phoh family Thewi_1055 ENOG4105CTJ Metal Dependent Phosphohydrolase Thewi_1056 ENOG4105KGE Single strand-specific metallo-endoribonuclease involved in late-stage 70S ribosome quality control and in maturation of the 3' terminus of the 16S rRNA (By similarity) Thewi_1059 ENOG4105CWT An essential GTPase that binds both GDP and GTP, with rapid nucleotide exchange. Plays a role in 16S rRNA processing and 30S ribosomal subunit biogenesis and possibly also in cell cycle regulation and energy metabolism (By similarity) Thewi_1063 ENOG4105D9H Catalyzes the attachment of glycine to tRNA(Gly) (By similarity) Thewi_1065 ENOG4105CF9 Bifunctional serine threonine kinase and phosphorylase involved in the regulation of the pyruvate, phosphate dikinase (PPDK) by catalyzing its phosphorylation dephosphorylation (By similarity) Thewi_1066 ENOG4108HRN Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate (By similarity) Thewi_1068 ENOG4105C40 3'-5' exoribonuclease that releases 5'-nucleoside monophosphates and is involved in maturation of structured RNAs (By similarity) Thewi_1072 ENOG4108KN7 Removes 5-oxoproline from various penultimate amino acid residues except L-proline (By similarity) Thewi_1075 ENOG4108Z0Z intracellular protease Pfpi family Thewi_1076 ENOG4108MP6 LysR family Transcriptional regulator Thewi_1078 ENOG4107S9D Alpha beta hydrolase Thewi_1086 ENOG4108QXE glycerophosphoryl diester phosphodiesterase Thewi_1088 ENOG4108UVJ appr-1-p processing domain protein Thewi_1089 ENOG4105DWR Catalyzes the dephosphorylation of undecaprenyl diphosphate (UPP). Confers resistance to bacitracin (By similarity) Thewi_1092 ENOG41066FP Small acid-soluble spore protein I Thewi_1095 ENOG4105CNW domain protein Thewi_1096 ENOG4108UXE Protein tyrosine phosphatase Thewi_1099 ENOG4105D8W glucose-6-phosphate 1-dehydrogenase Thewi_1100 ENOG4107XCH Cyclase family Thewi_1103 ENOG4105EJQ L-serine dehydratase Thewi_1104 ENOG4105DPN Oligoendopeptidase f Thewi_1105 ENOG4105D49 3-dehydroquinate synthase Thewi_1106 ENOG4105CQC Prephenate dehydratase Thewi_1107 ENOG4108JPM phospho-2-dehydro-3-deoxyheptonate aldolase Thewi_1108 ENOG4105EVN Prephenate dehydrogenase Thewi_1109 ENOG4105CMY 5-enolpyruvylshikimate-3-phosphate synthase Thewi_1110 ENOG4105CK6 Two component transcriptional regulator (Winged helix family Thewi_1111 ENOG41062D9 Histidine kinase Thewi_1117 ENOG4105DPZ Two component transcriptional regulator (Winged helix family Thewi_1118 ENOG4105EB3 Histidine kinase Thewi_1124 ENOG4105CKA Catalyzes the reversible isomerization-deamination of glucosamine 6-phosphate (GlcN6P) to form fructose 6-phosphate (Fru6P) and ammonium ion (By similarity) Thewi_1127 ENOG4105D6C Part of the ABC transporter complex MacAB involved in macrolide export. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation (By similarity) Thewi_1129 ENOG4107QIV Cytochrome c biogenesis protein Thewi_1130 ENOG4107QXG alkyl hydroperoxide reductase Thiol specific antioxidant Mal allergen Thewi_1131 ENOG4105K63 Thioredoxin Thewi_1132 ENOG4105CXN decarboxylase Thewi_1142 ENOG4105C1N GTPase that associates with the 50S ribosomal subunit and may have a role during protein synthesis or ribosome biogenesis (By similarity) Thewi_1145 ENOG4108ZK7 protein family UPF0029, Impact, N-terminal protein Thewi_1150 ENOG4105C6T cysteine synthase Thewi_1151 ENOG4105CDY transcriptional regulatory protein Thewi_1152 ENOG4108V3W Chemotaxis protein, CheW Thewi_1154 ENOG4105NHU Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group (By similarity) Thewi_1155 ENOG4105KA4 The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing. RuvA stimulates, in the presence of DNA, the weak ATPase activity of RuvB (By similarity) Thewi_1156 ENOG4105CKJ The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing (By similarity) Thewi_1157 ENOG41061AI Protein of unknown function (DUF2905) Thewi_1158 ENOG4105JFG SpoIID LytB domain protein Thewi_1160 ENOG4105C6U Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis- dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) (By similarity) Thewi_1165 ENOG4108Z0R Transferase Thewi_1166 ENOG4105C3D Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. SecDF uses the proton motive force (PMF) to complete protein translocation after the ATP-dependent function of SecA (By similarity) Thewi_1167 ENOG4107RAB Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. SecDF uses the proton motive force (PMF) to complete protein translocation after the ATP-dependent function of SecA (By similarity) Thewi_1169 ENOG4105C2R Single-stranded-DNA-specific exonuclease (RecJ) Thewi_1170 ENOG4109003 Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis (By similarity) Thewi_1171 ENOG4105CWR In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance (By similarity) Thewi_1173 ENOG4107VMB Beta-lactamase domain protein Thewi_1174 ENOG4107RBB ABC transporter, permease Thewi_1175 ENOG4105D6C Part of the ABC transporter complex MacAB involved in macrolide export. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation (By similarity) Thewi_1177 ENOG41067TZ transcriptional regulator, MarR family Thewi_1179 ENOG4105CUT histidyl-tRNA synthetase Thewi_1180 ENOG4105C9M aspartyl-trna synthetase Thewi_1181 ENOG4105CUZ uba thif-type nad fad binding protein Thewi_1182 ENOG4105VPX protein, conserved in bacteria Thewi_1183 ENOG4105K63 Thioredoxin Thewi_1184 ENOG4108KR4 dihydrodipicolinate reductase Thewi_1185 ENOG4105Z5Q Thewi_1186 ENOG4107RH1 pyridoxal-5'-phosphate-dependent protein beta subunit Thewi_1187 ENOG4105KIJ D-ornithine aminomutase S component Thewi_1188 ENOG4105EQ5 D-Lysine 5,6-aminomutase alpha subunit Thewi_1195 ENOG4105C02 permease Thewi_1196 ENOG4105CIM Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction alanine is first activated by ATP to form Ala- AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain (By similarity) Thewi_1197 ENOG4105VBH UPF0297 protein Thewi_1198 ENOG4108Z08 Could be a nuclease that resolves Holliday junction intermediates in genetic recombination (By similarity) Thewi_1199 ENOG41085AS UPF0473 protein Thewi_1200 ENOG4105I3K Ferric uptake regulator, Fur family Thewi_1201 ENOG4105CN5 Metallo-Beta-Lactamase Thewi_1202 ENOG41084CE UPF0342 protein Thewi_1203 ENOG410807C QueT transporter Thewi_1204 ENOG4105HHA aminodeoxychorismate lyase Thewi_1205 ENOG4108VQJ O-methyltransferase Thewi_1206 ENOG4105CFP Peptidase U32 Thewi_1207 ENOG4105CJN penicillin-binding protein Thewi_1208 ENOG4105DMD Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released (By similarity) Thewi_1209 ENOG4108VXP Specifically catalyzes the dephosphorylation of 2- phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stress (By similarity) Thewi_1210 ENOG4105E2X shikimate dehydrogenase Thewi_1211 ENOG4107QHF type ii secretion system protein e Thewi_1212 ENOG4105CZX twitching motility protein Thewi_1214 ENOG4106855 Competence protein Thewi_1224 ENOG4105KHV Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate (By similarity) Thewi_1226 ENOG4105CGT Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline (By similarity) Thewi_1227 ENOG4105C2S Catalyzes the NADPH dependent reduction of L-gamma- glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate (By similarity) Thewi_1228 ENOG4108Z38 Catalyzes a trans-dehydration via an enolate intermediate (By similarity) Thewi_1229 ENOG4105DC7 peptidase M24 Thewi_1230 ENOG4105DRH Involved in peptide bond synthesis. Stimulates efficient translation and peptide-bond synthesis on native or reconstituted 70S ribosomes in vitro. Probably functions indirectly by altering the affinity of the ribosome for aminoacyl-tRNA, thus increasing their reactivity as acceptors for peptidyl transferase (By similarity) Thewi_1232 ENOG4105CD2 stage iii sporulation protein aa Thewi_1234 ENOG4108CQH stage III sporulation protein AC Thewi_1235 ENOG4105KQ4 Stage iii sporulation protein ad Thewi_1237 ENOG410644B Stage III sporulation protein af Thewi_1240 ENOG4108ZSR alkaline shock protein Thewi_1241 ENOG4105M7K Involved in the transcription termination process (By similarity) Thewi_1242 ENOG4105CN0 Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate (By similarity) Thewi_1243 ENOG4105DVS Bidirectionally degrades single-stranded DNA into large acid-insoluble oligonucleotides, which are then degraded further into small acid-soluble oligonucleotides (By similarity) Thewi_1244 ENOG41067QG Bidirectionally degrades single-stranded DNA into large acid-insoluble oligonucleotides, which are then degraded further into small acid-soluble oligonucleotides (By similarity) Thewi_1245 ENOG4105CTB Polyprenyl synthetase Thewi_1247 ENOG4105C2V Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP) (By similarity) Thewi_1248 ENOG4105DX0 Hemolysin A Thewi_1249 ENOG4105F91 Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus (By similarity) Thewi_1250 ENOG4105XBY Regulates arginine biosynthesis genes (By similarity) Thewi_1251 ENOG4105C4F May be involved in recombinational repair of damaged DNA (By similarity) Thewi_1252 ENOG4105S1Z Stage IV sporulation protein B Thewi_1253 ENOG4107QQR Sporulation Thewi_1262 ENOG4105MZ9 stage II sporulation protein m Thewi_1264 ENOG4105DZ1 carboxypeptidase Thewi_1266 ENOG4108ZBE Binds to sigma F and blocks its ability to form an RNA polymerase holoenzyme (E-sigma F). Phosphorylates SpoIIAA on a serine residue. This phosphorylation may enable SpoIIAA to act as an anti-anti-sigma factor that counteracts SpoIIAB and thus releases sigma F from inhibition (By similarity) Thewi_1267 ENOG4105CT2 Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released (By similarity) Thewi_1268 ENOG41084UG Pfam:DUF1458 Thewi_1269 ENOG41090VF Stage v sporulation protein ac Thewi_1272 ENOG4105D4J Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate Thewi_1273 ENOG4108Z4T Peptidase M50 Thewi_1274 ENOG4108KVX Participates in chromosomal partition during cell division. May act via the formation of a condensin-like complex containing Smc and ScpB that pull DNA away from mid-cell into both cell halves (By similarity) Thewi_1275 ENOG4105K8I Participates in chromosomal partition during cell division. May act via the formation of a condensin-like complex containing Smc and ScpA that pull DNA away from mid-cell into both cell halves (By similarity) Thewi_1280 ENOG4105KBJ sporulation protein ytfj Thewi_1282 ENOG4105DZ1 carboxypeptidase Thewi_1285 ENOG4107RE5 NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34 (By similarity) Thewi_1286 ENOG4105FE9 pseudouridine synthase Thewi_1288 ENOG4105CCX Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor (By similarity) Thewi_1289 ENOG4105CYW agmatinase Thewi_1290 ENOG4107QSA Oxaloacetate decarboxylase Thewi_1293 ENOG4105CV2 Nucleotidase that shows phosphatase activity on nucleoside 5'-monophosphates (By similarity) Thewi_1295 ENOG4105DI2 Pyruvate flavodoxin ferredoxin oxidoreductase domain protein Thewi_1296 ENOG4105DQU oxidoreductase, subunit beta Thewi_1298 ENOG4108PSC Represses the expression of the murPQ operon involved in the uptake and degradation of N-acetylmuramic acid (MurNAc). Binds to two adjacent inverted repeats within the operator region. MurNAc 6-phosphate, the substrate of MurQ, is the specific inducer that weakens binding of MurR to the operator (By similarity) Thewi_1299 ENOG4105D82 Glutamate dehydrogenase Thewi_1301 ENOG4105CVJ phosphoglycerate mutase Thewi_1302 ENOG4105CBX hi0933 family Thewi_1304 ENOG4105KHN chorismate mutase Thewi_1305 ENOG4105CAT Cytidine monophosphate kinase Thewi_1307 ENOG4108IUH Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) (By similarity) Thewi_1308 ENOG4105CAV thus facilitating recognition of the initiation point. It is needed to translate mRNA with a short Shine-Dalgarno (SD) purine-rich sequence (By similarity) Thewi_1310 ENOG4105X3E Catalyzes the decarboxylation of S-adenosylmethionine to S-adenosylmethioninamine (dcAdoMet), the propylamine donor required for the synthesis of the polyamines spermine and spermidine from the diamine putrescine (By similarity) Thewi_1311 ENOG4105BZU Histidine kinase Thewi_1312 ENOG4105CIW Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine (By similarity) Thewi_1313 ENOG4105D86 that it carries out the mismatch recognition step. This protein has a weak ATPase activity (By similarity) Thewi_1315 ENOG4105DKX Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A) (By similarity) Thewi_1316 ENOG4105KW8 RNA chaperone that binds small regulatory RNA (sRNAs) and mRNAs to facilitate mRNA translational regulation in response to envelope stress, environmental stress and changes in metabolite concentrations. Also binds with high specificity to tRNAs (By similarity) Thewi_1317 ENOG4105II7 pyrroline-5-carboxylate reductase Thewi_1318 ENOG4108UMW Endonuclease that specifically degrades the RNA of RNA- DNA hybrids (By similarity) Thewi_1319 ENOG4108IYT Site-specific tyrosine recombinase, which acts by catalyzing the cutting and rejoining of the recombining DNA molecules. The XerC-XerD complex is essential to convert dimers of the bacterial chromosome into monomers to permit their segregation at cell division. It also contributes to the segregational stability of plasmids (By similarity) Thewi_1320 ENOG4105CXI aluminum resistance protein Thewi_1321 ENOG4106F1P AAA ATPase, central domain protein Thewi_1324 ENOG4105DB7 PHP domain protein Thewi_1325 ENOG4105MCT stage V sporulation protein S Thewi_1326 ENOG4105D48 metallophosphoesterase Thewi_1330 ENOG410902V Modulates RecA activity (By similarity) Thewi_1331 ENOG4105C68 Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with LexA causing its activation and leading to its autocatalytic cleavage (By similarity) Thewi_1332 ENOG4105CEV competence damage-inducible protein Thewi_1333 ENOG4105BZQ cdp-diacylglycerol-glycerol-3-phosphate 3-phosphatidyltransferase Thewi_1334 ENOG4105CBM Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12 (By similarity) Thewi_1337 ENOG4105CVQ ATP-dependent Clp protease, proteolytic subunit Thewi_1340 ENOG4108Z1K This enzyme is involved in nucleotide metabolism it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA (By similarity) Thewi_1341 ENOG4105C1V Peptidase, M16 Thewi_1343 ENOG4105C62 Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction (By similarity) Thewi_1344 ENOG4105K77 Forms an intersubunit bridge (bridge B4) with the 23S rRNA of the 50S subunit in the ribosome (By similarity) Thewi_1346 ENOG4105D0T Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs (By similarity) Thewi_1347 ENOG4105SFS phosphoesterase RecJ domain protein Thewi_1348 ENOG4105KG7 Associates with free 30S ribosomal subunits (but not with 30S subunits that are part of 70S ribosomes or polysomes). Essential for efficient processing of 16S rRNA. May interact with the 5'-terminal helix region of 16S rRNA (By similarity) Thewi_1351 ENOG4105VZN Nucleic-acid-binding protein implicated in transcription termination Thewi_1352 ENOG4105CHV Transcription elongation factor NusA Thewi_1353 ENOG4105K7D Required for maturation of 30S ribosomal subunits (By similarity) Thewi_1354 ENOG4105DE5 Required for replicative DNA synthesis. This DNA polymerase also exhibits 3' to 5' exonuclease activity (By similarity) Thewi_1356 ENOG4105C4E Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate (By similarity) Thewi_1357 ENOG4105DZP Membrane-associated zinc metalloprotease Thewi_1358 ENOG4105CEA Catalyzes the NADP-dependent rearrangement and reduction of 1-deoxy-D-xylulose-5-phosphate (DXP) to 2-C-methyl-D-erythritol 4-phosphate (MEP) (By similarity) Thewi_1359 ENOG4105KNE Phosphatidate cytidylyltransferase Thewi_1360 ENOG4105CR3 Catalyzes the condensation of isopentenyl diphosphate (IPP) with allylic pyrophosphates generating different type of terpenoids (By similarity) Thewi_1362 ENOG4108VCV Responsible for the release of ribosomes from messenger RNA at the termination of protein biosynthesis. May increase the efficiency of translation by recycling ribosomes from one round of translation to another (By similarity) Thewi_1363 ENOG4105C41 Catalyzes the reversible phosphorylation of UMP to UDP (By similarity) Thewi_1364 ENOG4105CU7 Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome (By similarity) Thewi_1368 ENOG4105DN4 Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released (By similarity) Thewi_1369 ENOG4108Z4W Probably deamidates glutamine residues to glutamate on methyl-accepting chemotaxis receptors (MCPs), playing an important role in chemotaxis (By similarity) Thewi_1371 ENOG4108V3W Chemotaxis protein, CheW Thewi_1372 ENOG4105CBS Chea signal transduction histidine kinase Thewi_1373 ENOG4105CMP catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR (By similarity) Thewi_1376 ENOG4105FZW flagellar biosynthesis regulator FlhF Thewi_1377 ENOG4105D7B Flagellar biosynthesis protein flha Thewi_1378 ENOG4105D00 flagellar biosynthetic protein flhB Thewi_1379 ENOG4105D19 Role in flagellar biosynthesis (By similarity) Thewi_1380 ENOG4105VS8 Flagellar biosynthetic protein fliq Thewi_1381 ENOG4105CN7 Flagellar biosynthetic protein FliP Thewi_1383 ENOG4107YTP response regulator receiver protein Thewi_1384 ENOG4105DD5 Flagellar motor switch protein Thewi_1385 ENOG4105D24 FliM is one of three proteins (FliG, FliN, FliM) that forms the rotor-mounted switch complex (C ring), located at the base of the basal body. This complex interacts with the CheY and CheZ chemotaxis proteins, in addition to contacting components of the motor that determine the direction of flagellar rotation (By similarity) Thewi_1387 ENOG41068WV flagellar FlbD family protein Thewi_1388 ENOG4105BZE flagellar hook protein, FlgE Thewi_1390 ENOG410822I Flagellar hook capping protein Thewi_1396 ENOG4105CS3 FliG is one of three proteins (FliG, FliN, FliM) that forms the rotor-mounted switch complex (C ring), located at the base of the basal body. This complex interacts with the CheY and CheZ chemotaxis proteins, in addition to contacting components of the motor that determine the direction of flagellar rotation (By similarity) Thewi_1398 ENOG4105VQ0 flagellar hook-basal body complex protein fliE Thewi_1399 ENOG4108ZC9 flagellar basal-body rod protein flgc Thewi_1400 ENOG4105Q75 Structural component of flagellum, the bacterial motility apparatus. Part of the rod structure of flagellar basal body (By similarity) Thewi_1401 ENOG4105EUM DNA-binding protein that represses the expression of many genes that are induced as cells make the transition from rapid exponential growth to stationary phase. It is a GTP-binding protein that senses the intracellular GTP concentration as an indicator of nutritional limitations. At low GTP concentration it no longer binds GTP and stop to act as a transcriptional repressor (By similarity) Thewi_1403 ENOG4108R5P Protease subunit of a proteasome-like degradation complex believed to be a general protein degrading machinery (By similarity) Thewi_1404 ENOG4105C73 Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity) Thewi_1406 ENOG4108UH2 Endonuclease that specifically degrades the RNA of RNA- DNA hybrids (By similarity) Thewi_1407 ENOG4105VFF UPF0102 protein Thewi_1408 ENOG4105C31 Tryptophanyl-tRNA synthetase Thewi_1409 ENOG4108JYZ YitT family Thewi_1410 ENOG4105C0P Mg chelatase subunit ChlI Thewi_1412 ENOG4108YY1 This protein is located at the 30S-50S ribosomal subunit interface and may play a role in the structure and function of the aminoacyl-tRNA binding site (By similarity) Thewi_1413 ENOG4105D6X Specifically methylates guanosine-37 in various tRNAs (By similarity) Thewi_1414 ENOG4107Z8R An accessory protein needed during the final step in the assembly of 30S ribosomal subunit, possibly for assembly of the head region. Probably interacts with S19. Essential for efficient processing of 16S rRNA. May be needed both before and after RbfA during the maturation of 16S rRNA. It has affinity for free ribosomal 30S subunits but not for 70S ribosomes (By similarity) Thewi_1415 ENOG4105VB6 UPF0109 protein Thewi_1416 ENOG4105K5M 30s ribosomal protein S16 Thewi_1418 ENOG4105VBN Might take part in the signal recognition particle (SRP) pathway. This is inferred from the conservation of its genetic proximity to ftsY ffh. May be a regulatory protein (By similarity) Thewi_1419 ENOG4105CCP Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Acts as a receptor for the complex formed by the signal recognition particle (SRP) and the ribosome-nascent chain (RNC) Thewi_1422 ENOG4105MCT stage V sporulation protein S Thewi_1424 ENOG4108ZBM Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Also processes some mRNAs, and tRNAs when they are encoded in the rRNA operon (By similarity) Thewi_1425 ENOG4105C0Q Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP (By similarity) Thewi_1426 ENOG4105VNB Carrier of the growing fatty acid chain in fatty acid biosynthesis (By similarity) Thewi_1427 ENOG4105CHR reductase Thewi_1428 ENOG4105CJF Malonyl coA-acyl carrier protein transacylase Thewi_1429 ENOG4105EA8 2-Nitropropane dioxygenase Thewi_1430 ENOG4105CCZ Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Catalyzes the first condensation reaction which initiates fatty acid synthesis and may therefore play a role in governing the total rate of fatty acid production. Possesses both acetoacetyl-ACP synthase and acetyl transacylase activities. Its substrate specificity determines the biosynthesis of branched- chain and or straight-chain of fatty acids (By similarity) Thewi_1431 ENOG4105C6B Catalyzes the reversible formation of acyl-phosphate (acyl-PO(4)) from acyl- acyl-carrier-protein (acyl-ACP). This enzyme utilizes acyl-ACP as fatty acyl donor, but not acyl-CoA (By similarity) Thewi_1432 ENOG4108VVV Transcriptional factor involved in regulation of membrane lipid biosynthesis by repressing genes involved in fatty acid and phospholipid metabolism (By similarity) Thewi_1433 ENOG41067VY 50S ribosomal protein L32 Thewi_1434 ENOG4105KNH Nucleic acid-binding protein Thewi_1435 ENOG4105C6H Catalyzes the formation of acetyl phosphate from acetate and ATP. Can also catalyze the reverse reaction (By similarity) Thewi_1436 ENOG4108IJ2 phosphate acetyltransferase Thewi_1438 ENOG4105H20 Sporulation integral membrane protein YlbJ Thewi_1440 ENOG4108ZEF Reversibly transfers an adenylyl group from ATP to 4'- phosphopantetheine, yielding dephospho-CoA (dPCoA) and pyrophosphate (By similarity) Thewi_1441 ENOG4106H78 methyltransferase Thewi_1444 ENOG4105EPR degv family Thewi_1445 ENOG4105CB5 ATP-dependent DNA helicase recG Thewi_1446 ENOG4105DP4 dak2 domain fusion protein ylov Thewi_1447 ENOG4108ZAA Alkaline-shock protein Thewi_1452 ENOG4105E06 May play a role in 30S ribosomal subunit biogenesis. Unusual circulary permuted GTPase that catalyzes rapid hydrolysis of GTP with a slow catalytic turnover (By similarity) Thewi_1453 ENOG4105D9P serine threonine protein kinase Thewi_1454 ENOG4108ZF3 Phosphatase Thewi_1455 ENOG4105C55 Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs Thewi_1456 ENOG4105CYJ ribosomal RNA small subunit methyltransferase B Thewi_1459 ENOG4105CAE Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by (I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-GTP (By similarity) Thewi_1460 ENOG4108Z02 Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions (By similarity) Thewi_1461 ENOG4105C25 Primosomal protein n' Thewi_1462 ENOG4105CJS Phosphopantothenoylcysteine decarboxylase Thewi_1464 ENOG4108UHA Essential for recycling GMP and indirectly, cGMP (By similarity) Thewi_1465 ENOG4105KC4 UPF0296 protein Thewi_1466 ENOG4105DSJ YicC domain protein Thewi_1467 ENOG4105E4Z Catalyzes the stereoinversion of LL-2,6- diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso- DAP), a precursor of L-lysine and an essential component of the bacterial peptidoglycan (By similarity) Thewi_1468 ENOG4105C6X Major Facilitator Thewi_1470 ENOG4105F23 Fibronectin-binding protein Thewi_1473 ENOG4108JEI Part of the ABC transporter complex MetNIQ involved in methionine import. Responsible for energy coupling to the transport system (By similarity) Thewi_1474 ENOG4108UMI ABC transporter, permease Thewi_1475 ENOG4105D01 (LipO)protein Thewi_1477 ENOG4105CB3 Required for the thiolation of cytidine in position 32 of tRNA, to form 2-thiocytidine (s(2)C32) (By similarity) Thewi_1480 ENOG4107XPQ Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) (By similarity) Thewi_1482 ENOG4105DF0 Responsible for channeling the electrons from the oxidation of dihydroorotate from the FMN redox center in the PyrD type B subunit to the ultimate electron acceptor NAD( ) (By similarity) Thewi_1484 ENOG4105CD3 dihydroorotase Thewi_1485 ENOG4105CXT aspartate transcarbamylase Thewi_1486 ENOG4108UK1 Also displays a weak uracil phosphoribosyltransferase activity which is not physiologically significant (By similarity) Thewi_1487 ENOG4108JIU phosphoribosylformylglycinamidine synthase ii Thewi_1488 ENOG4105C34 Pseudouridine synthase Thewi_1489 ENOG4105M02 This protein specifically catalyzes the removal of signal peptides from prolipoproteins (By similarity) Thewi_1501 ENOG4105M2E Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell (By similarity) Thewi_1503 ENOG4108TVJ Nitroreductase Thewi_1504 ENOG4108AEY RNA polymerase sigma factor Thewi_1507 ENOG41067S9 phage shock protein C, PspC Thewi_1511 ENOG4105C28 cystathionine Thewi_1516 ENOG4108YXY alkyl hydroperoxide reductase Thiol specific antioxidant Mal allergen Thewi_1517 ENOG4108JPM phospho-2-dehydro-3-deoxyheptonate aldolase Thewi_1521 ENOG4105DK0 Indole-3-glycerol phosphate synthase Thewi_1522 ENOG4108I0Q anthranilate phosphoribosyltransferase Thewi_1523 ENOG4105DDQ anthranilate synthase Thewi_1526 ENOG4105C28 cystathionine Thewi_1527 ENOG4105CB1 The purine nucleoside phosphorylases catalyze the phosphorolytic breakdown of the N-glycosidic bond in the beta- (deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1-phosphate (By similarity) Thewi_1528 ENOG4105C35 Catalyzes the interconversion of methylthioribose-1- phosphate (MTR-1-P) into methylthioribulose-1-phosphate (MTRu-1-P) (By similarity) Thewi_1529 ENOG4107W7X Aldolase Thewi_1530 ENOG4105D6T Catalyzes the deamination of 5-methylthioadenosine and S-adenosyl-L-homocysteine into 5-methylthioinosine and S-inosyl-L- homocysteine, respectively. Is also able to deaminate adenosine (By similarity) Thewi_1531 ENOG4105C07 amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) (By similarity) Thewi_1534 ENOG4105DBK s4 domain protein Thewi_1536 ENOG4105VR5 Cell division protein that is part of the divisome complex and is recruited early to the Z-ring. Probably stimulates Z-ring formation, perhaps through the cross-linking of FtsZ protofilaments. Its function overlaps with FtsA (By similarity) Thewi_1537 ENOG4105DFA alanine racemase domain protein Thewi_1540 ENOG4105KME endoribonuclease L-psp Thewi_1543 ENOG4105DTQ mandelate racemase muconate lactonizing Thewi_1547 ENOG4105C6Z Gluconate Thewi_1548 ENOG4105BZW Glycerate kinase Thewi_1552 ENOG4105D1F ATP-binding protein Thewi_1560 ENOG4105C0R drug resistance transporter emrb qaca subfamily Thewi_1562 ENOG4105CJ1 ABC, transporter Thewi_1564 ENOG4105CI6 Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. Has a central role in coupling the hydrolysis of ATP to the transfer of proteins into and across the cell membrane, serving Thewi_1565 ENOG4105C0X amino acid Thewi_1571 ENOG4108VYJ response regulator receiver protein Thewi_1574 ENOG4105DXU ATPase. Has a role at an early stage in the morphogenesis of the spore coat (By similarity) Thewi_1576 ENOG4105K7Z Catalyzes the transfer of an acyl group from acyl- phosphate (acyl-PO(4)) to glycerol-3-phosphate (G3P) to form lysophosphatidic acid (LPA). This enzyme utilizes acyl-phosphate as fatty acyl donor, but not acyl-CoA or acyl-ACP (By similarity) Thewi_1577 ENOG4105DKZ GTPase that plays an essential role in the late steps of ribosome biogenesis (By similarity) Thewi_1582 ENOG4105S2F Thewi_1585 ENOG4108UTW Plays a role in the regulation of phosphate uptake Thewi_1586 ENOG4105BZY Part of the ABC transporter complex PstSACB involved in phosphate import. Responsible for energy coupling to the transport system (By similarity) Thewi_1587 ENOG4105E2Z phosphate ABC transporter (Permease Thewi_1588 ENOG4105C8U phosphate abc transporter Thewi_1589 ENOG4105CVA phosphate Thewi_1591 ENOG4105DPZ Two component transcriptional regulator (Winged helix family Thewi_1592 ENOG4108Z11 Negatively regulates transcription of bacterial ribonucleotide reductase nrd genes and operons by binding to NrdR- boxes (By similarity) Thewi_1594 ENOG4108BIK prc-barrel domain-containing protein Thewi_1595 ENOG4105E65 Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released (By similarity) Thewi_1596 ENOG4105DAV Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released (By similarity) Thewi_1598 ENOG4105CDK Essential cell division protein that forms a contractile ring structure (Z ring) at the future cell division site. The regulation of the ring assembly controls the timing and the location of cell division. One of the functions of the FtsZ ring is to recruit other cell division proteins to the septum to produce a new cell wall between the dividing cells. Binds GTP and shows GTPase activity (By similarity) Thewi_1599 ENOG4105CIT This protein may be involved in anomalous filament growth. May be a component of the septum (By similarity) Thewi_1600 ENOG4105K67 small basic protein Thewi_1603 ENOG4105CDF Cell wall formation. Adds enolpyruvyl to UDP-N- acetylglucosamine (By similarity) Thewi_1604 ENOG4105DVQ Cell wall formation. Catalyzes the transfer of a GlcNAc subunit on undecaprenyl-pyrophosphoryl-MurNAc-pentapeptide (lipid intermediate I) to form undecaprenyl-pyrophosphoryl-MurNAc- (pentapeptide)GlcNAc (lipid intermediate II) (By similarity) Thewi_1605 ENOG4105CNI cell division protein FtsW Thewi_1606 ENOG4105DMZ Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) (By similarity) Thewi_1607 ENOG4105CPY First step of the lipid cycle reactions in the biosynthesis of the cell wall peptidoglycan (By similarity) Thewi_1608 ENOG4107EES Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide the precursor of murein (By similarity) Thewi_1609 ENOG4107EEN Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan (By similarity) Thewi_1610 ENOG4105CJN penicillin-binding protein Thewi_1621 ENOG4105D8I Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS (By similarity) Thewi_1624 ENOG4105EKQ RNA-directed DNA polymerase Thewi_1627 ENOG4107QKR Dehydrogenase Thewi_1628 ENOG4106Z86 ATP-nad acox kinase Thewi_1630 ENOG4107RUY (E1 component), alpha subunit Thewi_1631 ENOG4105CJ9 Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions (By similarity) Thewi_1634 ENOG4105C0G Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives (By similarity) Thewi_1635 ENOG4108BDP Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate (By similarity) Thewi_1640 ENOG4105K4I Scp-like extracellular Thewi_1641 ENOG4105DFM M18 family aminopeptidase Thewi_1642 ENOG4105WH1 Two component transcriptional regulator luxr family Thewi_1645 ENOG4105EWC Purine nucleoside phosphorylase Thewi_1646 ENOG4105C98 muts2 protein Thewi_1647 ENOG4105CFP Peptidase U32 Thewi_1649 ENOG4105C6A phenylalanyl-tRNA synthetase (beta subunit) Thewi_1650 ENOG4105CSS phenylalanyl-tRNA synthetase (alpha subunit) Thewi_1653 ENOG4108YZX Binds directly to 23S ribosomal RNA and is necessary for the in vitro assembly process of the 50S ribosomal subunit. It is not involved in the protein synthesizing functions of that subunit (By similarity) Thewi_1654 ENOG4105VJV 50s ribosomal protein L35 Thewi_1655 ENOG4108UUX IF-3 binds to the 30S ribosomal subunit and shifts the equilibrum between 70S ribosomes and their 50S and 30S subunits in favor of the free subunits, thus enhancing the availability of 30S subunits on which protein synthesis initiation begins (By similarity) Thewi_1657 ENOG4105D10 5-enolpyruvylshikimate-3-phosphate phospholyase Thewi_1659 ENOG4105C22 threonyL-tRNA synthetase Thewi_1660 ENOG4108UH6 UPF0754 membrane protein Thewi_1663 ENOG41065U4 response regulator Thewi_1664 ENOG4105XXY Thioredoxin Thewi_1665 ENOG4105F4C Redox regulated molecular chaperone. Protects both thermally unfolding and oxidatively damaged proteins from irreversible aggregation. Plays an important role in the bacterial defense system toward oxidative stress (By similarity) Thewi_1666 ENOG4105VEQ Cold shock protein Thewi_1667 ENOG4105NDF NAD-dependent protein deacetylase which modulates the activities of several enzymes which are inactive in their acetylated form. May also have NAD-dependent lysine demalonylase and desuccinylase activity (By similarity) Thewi_1670 ENOG41083MR nifu domain-containing protein Thewi_1672 ENOG4105C3B Transfers the N-acyl diglyceride group on what will become the N-terminal cysteine of membrane lipoproteins (By similarity) Thewi_1675 ENOG4107QSE Dipeptidase Thewi_1677 ENOG4105DG6 mannose-6-phosphate isomerase Thewi_1678 ENOG4105D3A purine nucleoside phosphorylase DeoD-type Thewi_1679 ENOG4105EGD dinuclear metal center protein, YbgI family Thewi_1681 ENOG4105DG1 Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released (By similarity) Thewi_1682 ENOG4105C9G DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis (By similarity) Thewi_1683 ENOG4105C4Q deoxyguanosinetriphosphate triphosphohydrolase-like protein Thewi_1684 ENOG4105XZU Preprotein translocase, subunit SecG Thewi_1685 ENOG4105C70 Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis (By similarity) Thewi_1686 ENOG4105CJI Catalyzes the interconversion of 2-phosphoglycerate and 3-phosphoglycerate (By similarity) Thewi_1687 ENOG4105CP7 Triose-phosphate isomerase Thewi_1688 ENOG4105BZA Phosphoglycerate kinase Thewi_1691 ENOG4105DTW Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released (By similarity) Thewi_1692 ENOG4105VSY K01512 acylphosphatase EC 3.6.1.7 Thewi_1693 ENOG410667J hydrolase Thewi_1695 ENOG4105G5W regulator Fur family Thewi_1696 ENOG41073R8 VanW family Thewi_1697 ENOG4105CSM endonuclease III Thewi_1698 ENOG4105EH2 Catalyzes the conversion of epoxyqueuosine (oQ) to queuosine (Q), which is a hypermodified base found in the wobble positions of tRNA(Asp), tRNA(Asn), tRNA(His) and tRNA(Tyr) (By similarity) Thewi_1699 ENOG4105DAN ATP-dependent protease Thewi_1701 ENOG410688J Ferredoxin Thewi_1704 ENOG4105C8Q Methyl-accepting chemotaxis Thewi_1705 ENOG4105C22 threonyL-tRNA synthetase Thewi_1711 ENOG4108K9K Uncharacterised protein family (UPF0236) Thewi_1723 ENOG4105CTX dehydratase Thewi_1726 ENOG4105CMG Carbohydrate kinase Thewi_1732 ENOG4105CDP Catalyzes the condensation of (S)-aspartate-beta- semialdehyde (S)-ASA and pyruvate to 4-hydroxy- tetrahydrodipicolinate (HTPA) (By similarity) Thewi_1733 ENOG4105D2N Aldolase Thewi_1734 ENOG4105DB7 PHP domain protein Thewi_1735 ENOG4108VXP Specifically catalyzes the dephosphorylation of 2- phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stress (By similarity) Thewi_1736 ENOG4108YZG isomerase Thewi_1737 ENOG4108IUS Transketolase Thewi_1738 ENOG4108HHA Transketolase Thewi_1739 ENOG4107QQX Transaldolase is important for the balance of metabolites in the pentose-phosphate pathway (By similarity) Thewi_1742 ENOG4105EMS pseudouridine synthase Thewi_1745 ENOG4105DJ1 Phosphoenolpyruvate Carboxylase Thewi_1749 ENOG4108X8W Catalyzes the transfer of an acyl group from acyl- phosphate (acyl-PO(4)) to glycerol-3-phosphate (G3P) to form lysophosphatidic acid (LPA). This enzyme utilizes acyl-phosphate as fatty acyl donor, but not acyl-CoA or acyl-ACP (By similarity) Thewi_1750 ENOG41076QQ Glycosyl transferase, family 2 Thewi_1757 ENOG4105BZT Catalyzes the formation of 5-methyl-uridine at position 1939 (m5U1939) in 23S rRNA (By similarity) Thewi_1760 ENOG4105D9Q decarboxylase (Beta subunit) Thewi_1763 ENOG4105WEF catalase Thewi_1764 ENOG4108UIN ybak prolyl-trna synthetase associated region Thewi_1765 ENOG4105C3R Methionine synthase Thewi_1767 ENOG4107R76 Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties (By similarity) Thewi_1768 ENOG4107QYV phosphohexose isomerase Thewi_1770 ENOG4108RCM Nitroreductase Thewi_1771 ENOG4105D42 Cleaves the N-terminal amino acid of tripeptides (By similarity) Thewi_1772 ENOG4107XM9 (LipO)protein Thewi_1774 ENOG4105CYQ Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) (By similarity) Thewi_1776 ENOG4105E90 ABC transporter Thewi_1777 ENOG4108IJ8 ABC transporter Thewi_1779 ENOG4105UQX Mate efflux family protein Thewi_1783 ENOG4105BZT Catalyzes the formation of 5-methyl-uridine at position 1939 (m5U1939) in 23S rRNA (By similarity) Thewi_1787 ENOG4105CTQ phosphohexokinase Thewi_1789 ENOG4105C0B DNA polymerase III (alpha subunit) Thewi_1791 ENOG4105KRI phosphocarrier protein (HPr Thewi_1792 ENOG4105CIE May be required for sporulation (By similarity) Thewi_1794 ENOG4108IUK alpha amylase, catalytic Thewi_1798 ENOG4105EG2 ABC transporter, permease Thewi_1799 ENOG4105D56 permease protein Thewi_1800 ENOG4107FKK extracellular solute-binding protein family 1 Thewi_1805 ENOG4105D4A Cell wall formation (By similarity) Thewi_1807 ENOG4105D78 K07001 NTE family protein Thewi_1808 ENOG4108MHR Repressor involved in choline regulation of the bet genes (By similarity) Thewi_1814 ENOG4105C59 p-type ATPase Thewi_1815 ENOG4105CCJ Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s(2)U34 (By similarity) Thewi_1817 ENOG4105C6T cysteine synthase Thewi_1820 ENOG41082MP copper ion binding protein Thewi_1821 ENOG4105DQR SNARE associated Golgi Thewi_1822 ENOG4105VYW UPF0251 protein Thewi_1823 ENOG4105BZU Histidine kinase Thewi_1826 ENOG4105G5W regulator Fur family Thewi_1831 ENOG4105CE1 Ferrous iron transport protein b Thewi_1836 ENOG4108HWH (ABC) transporter Thewi_1837 ENOG4105EHG Permease protein Thewi_1840 ENOG4107QMW pyridine nucleotide-disulfide oxidoreductase Thewi_1841 ENOG4107QW8 radical SAM domain protein Thewi_1844 ENOG4108RW0 Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid (By similarity) Thewi_1846 ENOG4105D1F ATP-binding protein Thewi_1847 ENOG4105DSM The key enzymatic reactions in nitrogen fixation are catalyzed by the nitrogenase complex, which has 2 components the iron protein and the molybdenum-iron protein (By similarity) Thewi_1851 ENOG4105DDZ Methyltransferase required for the conversion of demethylmenaquinone (DMKH2) to menaquinone (MKH2) and the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2) (By similarity) Thewi_1855 ENOG4108JVH Protein of unknown function DUF43 Thewi_1856 ENOG4105ETE Transcriptional regulator (LacI family Thewi_1857 ENOG4105D7K pfkb domain protein Thewi_1858 ENOG4108HKT sucrose-6-phosphate hydrolase Thewi_1861 ENOG4107FKK extracellular solute-binding protein family 1 Thewi_1872 ENOG4105ECY inositol monophosphatase Thewi_1879 ENOG4106P19 photosystem i assembly btpa Thewi_1880 ENOG4105E98 transcriptional regulator DeoR family Thewi_1882 ENOG4105CI6 Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. Has a central role in coupling the hydrolysis of ATP to the transfer of proteins into and across the cell membrane, serving Thewi_1887 ENOG4105CJ1 ABC, transporter Thewi_1888 ENOG4105CJ1 ABC, transporter Thewi_1892 ENOG4105CNT hydrolase family 2 Thewi_1898 ENOG4105E2R ROK family Thewi_1899 ENOG4105CMR udp-glucose 4-epimerase Thewi_1900 ENOG4105CRD Catalyzes the transfer of the gamma-phosphate of ATP to D-galactose to form alpha-D-galactose-1-phosphate (Gal-1-P) (By similarity) Thewi_1901 ENOG4105CKN UDP-glucose-hexose-1-phosphate uridylyltransferase Thewi_1903 ENOG4108UHU Aldolase Thewi_1904 ENOG4105C5I Dehydrogenase Thewi_1906 ENOG4108IQ3 PTS system Thewi_1907 ENOG4108EQA pts system Thewi_1908 ENOG4105F3R pts system mannose fructose sorbose family iid Thewi_1910 ENOG4108EQA pts system Thewi_1912 ENOG4105C1W two component, sigma54 specific, transcriptional regulator, Fis family Thewi_1913 ENOG4105C7Q Catalyzes the oxidative decarboxylation of 6- phosphogluconate to ribulose 5-phosphate and CO(2), with concomitant reduction of NADP to NADPH (By similarity) Thewi_1915 ENOG4105C5I Dehydrogenase Thewi_1918 ENOG4105C5I Dehydrogenase Thewi_1923 ENOG4105DRI Involved in the oxidation of myo-inositol (MI) to 2- keto-myo-inositol (2KMI or 2-inosose) (By similarity) Thewi_1931 ENOG4105NBF ammecr1 domain protein Thewi_1933 ENOG4105CEY Amp-dependent synthetase and ligase Thewi_1936 ENOG4105D1C Catalyzes the ATP- as well as the pyrophosphate- dependent phosphorylation of a specific serine residue in HPr, a phosphocarrier protein of the phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS). HprK P also catalyzes the pyrophosphate-producing, inorganic phosphate-dependent dephosphorylation (phosphorolysis) of seryl-phosphorylated HPr (P- Ser-HPr). The two antagonistic activities of HprK P are regulated by several intracellular metabolites, which change their concentration in response to the absence or presence of rapidly metabolisable carbon sources (glucose, fructose, etc.) in the growth medium. Therefore, by controlling the phosphorylation state of HPr, HPrK P is a sensor enzyme that plays a major role in the regulation of carbon metabolism and sugar transport it mediates carbon catabolite repression (CCR), and regulates PTS-catalyzed carbohydrate uptake and inducer exclusion (By similarity) Thewi_1939 ENOG4105CJN penicillin-binding protein Thewi_1942 ENOG4105C5U The UvrABC repair system catalyzes the recognition and processing of DNA lesions. UvrA is an ATPase and a DNA-binding protein. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. When the presence of a lesion has been verified by UvrB, the UvrA molecules dissociate (By similarity) Thewi_1943 ENOG4105CCW damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed. This complex is subsequently bound by UvrC and the second UvrB is released. If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage (By similarity) Thewi_1944 ENOG4108N5B 2-phosphosulfolactate phosphatase Thewi_1947 ENOG4105DR5 peptidase Thewi_1948 ENOG4105CRP Part of the ABC transporter FtsEX involved in Thewi_1949 ENOG4105CAK Cell division ATP-binding protein ftsE Thewi_1950 ENOG4108IXP Carbohydrate diacid transcriptional activator CdaR Thewi_1955 ENOG4105KW6 This is one of the proteins that binds to the 5S RNA in the ribosome where it forms part of the central protuberance (By similarity) Thewi_1956 ENOG4105C68 Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with LexA causing its activation and leading to its autocatalytic cleavage (By similarity) Thewi_1959 ENOG41084HI prevent-host-death family Thewi_1966 ENOG4108K9K Uncharacterised protein family (UPF0236) Thewi_1977 ENOG4105CDJ oxidoreductase FAD NAD(P)-binding domain protein Thewi_1978 ENOG4105BZ8 glutamate synthase Thewi_1986 ENOG4108K9K Uncharacterised protein family (UPF0236) Thewi_1990 ENOG4105MN5 HAD-superfamily hydrolase subfamily IA variant 3 Thewi_1992 ENOG4105CIT This protein may be involved in anomalous filament growth. May be a component of the septum (By similarity) Thewi_2000 ENOG4105CFG Acts as a chaperone (By similarity) Thewi_2001 ENOG4105CY0 Metallo-Beta-Lactamase Thewi_2002 ENOG4105VDH Dihydroxyacetone kinase Thewi_2004 ENOG4105CMT Dihydroxyacetone kinase Thewi_2010 ENOG4105D8C Channel that permits osmotically driven movement of water in both directions. It is involved in the osmoregulation and in the maintenance of cell turgor during volume expansion in rapidly growing cells. It mediates rapid entry or exit of water in response to abrupt changes in osmolarity (By similarity) Thewi_2011 ENOG4108ZIA glycerol-3-phosphate responsive antiterminator Thewi_2017 ENOG4108R80 One of the components of the high-affinity ATP-driven potassium transport (or KDP) system, which catalyzes the hydrolysis of ATP coupled with the exchange of hydrogen and potassium ions. The C subunit may be involved in assembly of the KDP complex (By similarity) Thewi_2018 ENOG4105C8X One of the components of the high-affinity ATP-driven potassium transport (or KDP) system, which catalyzes the hydrolysis of ATP coupled with the exchange of hydrogen and potassium ions (By similarity) Thewi_2019 ENOG4105D9K One of the components of the high-affinity ATP-driven potassium transport (or KDP) system, which catalyzes the hydrolysis of ATP coupled with the exchange of hydrogen and potassium ions (By similarity) Thewi_2025 ENOG4108IGT Dipeptidase Thewi_2026 ENOG4105C65 Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF- independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism (By similarity) Thewi_2027 ENOG4105K8F Phosphoribosyl-amp cyclohydrolase Thewi_2030 ENOG4108UJE IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to HisF for the synthesis of IGP and AICAR (By similarity) Thewi_2031 ENOG4105ECC imidazole-glycerol-phosphate dehydratase Thewi_2032 ENOG4105CIH Imidazole acetol-phosphate transaminase Thewi_2033 ENOG4105CEK Catalyzes the sequential NAD-dependent oxidations of L- histidinol to L-histidinaldehyde and then to L-histidine (By similarity) Thewi_2042 ENOG4105CJ1 ABC, transporter Thewi_2043 ENOG4105E2V Glycosyltransferase 36 Thewi_2044 ENOG4105D7N magnesium and cobalt transport protein CorA Thewi_2047 ENOG4105CYJ ribosomal RNA small subunit methyltransferase B Thewi_2048 ENOG4105CK5 oxidoreductase Thewi_2049 ENOG4105DWV Homoserine O-trans-acetylase Thewi_2051 ENOG4105D6E homoserine dehydrogenase Thewi_2056 ENOG4105VCQ rubredoxin Thewi_2057 ENOG4105C08 Histidine kinase Thewi_2058 ENOG4105DFD Na H antiporter Thewi_2059 ENOG4105CTQ phosphohexokinase Thewi_2061 ENOG4108IUS Transketolase Thewi_2062 ENOG4108HHA Transketolase Thewi_2063 ENOG4108JYZ YitT family Thewi_2069 ENOG4108YZM Toxic component of a toxin-antitoxin (TA) module (By similarity) Thewi_2071 ENOG4105CJ4 Catalyzes the interconversion of L-alanine and D- alanine. May also act on other amino acids (By similarity) Thewi_2073 ENOG4105DR1 Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration (By similarity) Thewi_2074 ENOG41082V8 Transfers the 4'-phosphopantetheine moiety from coenzyme A to a Ser of acyl-carrier-protein (By similarity) Thewi_2081 ENOG4105EMN RNA polymerase sigma factor Thewi_2085 ENOG4105DA5 (ABC) transporter Thewi_2088 ENOG4105D3R alkyl hydroperoxide reductase Thewi_2095 ENOG4105D2N Aldolase Thewi_2096 ENOG4107UEK pfkb domain protein Thewi_2097 ENOG4105CNN ABC, transporter Thewi_2099 ENOG4105E9V Periplasmic binding protein LacI transcriptional regulator Thewi_2100 ENOG4105ETE Transcriptional regulator (LacI family Thewi_2113 ENOG4105CFZ Catalyzes the decarboxylation of four acetate groups of uroporphyrinogen-III to yield coproporphyrinogen-III (By similarity) Thewi_2114 ENOG4108Q52 Methyltransferase cognate corrinoid protein Thewi_2115 ENOG4107XVH Transcriptional regulator Thewi_2120 ENOG4105F3R pts system mannose fructose sorbose family iid Thewi_2121 ENOG4105ETZ PTS System Thewi_2122 ENOG4108UZY PTS System Thewi_2123 ENOG4105C1W two component, sigma54 specific, transcriptional regulator, Fis family Thewi_2130 ENOG4105F3R pts system mannose fructose sorbose family iid Thewi_2131 ENOG4105ETZ PTS System Thewi_2132 ENOG4108IQ3 PTS system Thewi_2134 ENOG4105FDP GntR family transcriptional regulator Thewi_2138 ENOG4105EGY DNA internalization-related competence protein ComEC Rec2 Thewi_2143 ENOG4105FDF Could be involved in DNA repair (By similarity) Thewi_2144 ENOG4107RYT DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair (By similarity) Thewi_2145 ENOG4108YXI Secondary thiamine-phosphate synthase enzyme Thewi_2156 ENOG4105C13 Site-specific tyrosine recombinase, which acts by catalyzing the cutting and rejoining of the recombining DNA molecules. The XerC-XerD complex is essential to convert dimers of the bacterial chromosome into monomers to permit their segregation at cell division. It also contributes to the segregational stability of plasmids (By similarity) Thewi_2159 ENOG4105C9G DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis (By similarity) Thewi_2168 ENOG4105C0D helicase Thewi_2175 ENOG4105C46 Catalyzes the first step in hexosamine metabolism, converting fructose-6P into glucosamine-6P using glutamine as a nitrogen source (By similarity) Thewi_2177 ENOG4105CEY Amp-dependent synthetase and ligase Thewi_2180 ENOG4107RE7 phosphate butyryltransferase Thewi_2186 ENOG4105CKM Branched-chain carboxylic acid kinase Thewi_2187 ENOG4107RDP Dehydrogenase Thewi_2188 ENOG4107RE7 phosphate butyryltransferase Thewi_2189 ENOG4105CKM Branched-chain carboxylic acid kinase Thewi_2191 ENOG4105C8B TIGR00159 family Thewi_2198 ENOG4105C7R Atpase associated with various cellular activities aaa_3 Thewi_2199 ENOG4107XGZ Hydrolase Thewi_2207 ENOG4105CGK Exodeoxyribonuclease III Thewi_2208 ENOG4108YZ8 Superoxide reductase Thewi_2209 ENOG4105CBS Chea signal transduction histidine kinase Thewi_2210 ENOG4105CBS Chea signal transduction histidine kinase Thewi_2211 ENOG4105CBS Chea signal transduction histidine kinase Thewi_2212 ENOG4105BZU Histidine kinase Thewi_2214 ENOG4105CMP catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR (By similarity) Thewi_2215 ENOG4108V3W Chemotaxis protein, CheW Thewi_2216 ENOG4107SDU Methyl-accepting chemotaxis Thewi_2217 ENOG4105DW3 Response regulator receiver modulated metal dependent phosphohydrolase Thewi_2218 ENOG4108K8K Transcriptional regulator Thewi_2220 ENOG4108UJD 30S ribosomal protein S9 Thewi_2221 ENOG4108UM5 This protein is one of the early assembly proteins of the 50S ribosomal subunit, although it is not seen to bind rRNA by itself. It is important during the early stages of 50S assembly (By similarity) Thewi_2222 ENOG4105DI7 Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs (By similarity) Thewi_2223 ENOG4105EK6 Transmembrane (T) component of an energy-coupling factor (ECF) ABC-transporter complex. Unlike classic ABC transporters this ECF transporter provides the energy necessary to transport a number of different substrates (By similarity) Thewi_2224 ENOG4108JJB ABC transporter Thewi_2225 ENOG4108JJB ABC transporter Thewi_2226 ENOG4108ZT0 50S ribosomal protein l17 Thewi_2227 ENOG4105CTF DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates (By similarity) Thewi_2228 ENOG4105G6W One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the body of the 30S subunit (By similarity) Thewi_2229 ENOG4108UHH Located on the platform of the 30S subunit, it bridges several disparate RNA helices of the 16S rRNA. Forms part of the Shine-Dalgarno cleft in the 70S ribosome (By similarity) Thewi_2230 ENOG4108Z04 Located at the top of the head of the 30S subunit, it contacts several helices of the 16S rRNA. In the 70S ribosome it contacts the 23S rRNA (bridge B1a) and protein L5 of the 50S subunit (bridge B1b), connecting the 2 subunits Thewi_2232 ENOG4105K9U however, it seems to stimulate more or less all the activities of the other two initiation factors, IF-2 and IF-3 (By similarity) Thewi_2235 ENOG4105CC8 Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism (By similarity) Thewi_2236 ENOG4105CGG The central subunit of the protein translocation channel SecYEG. Consists of two halves formed by TMs 1-5 and 6-10. These two domains form a lateral gate at the front which open onto the bilayer between TMs 2 and 7, and are clamped together by SecE at the back. The channel is closed by both a pore ring composed of hydrophobic SecY resides and a short helix (helix 2A) on the extracellular side of the membrane which forms a plug. The plug probably moves laterally to allow the channel to open. The ring and the pore may move independently (By similarity) Thewi_2237 ENOG4108UZ0 Binds to the 23S rRNA (By similarity) Thewi_2238 ENOG4108RA9 Located at the back of the 30S subunit body where it stabilizes the conformation of the head with respect to the body (By similarity) Thewi_2239 ENOG4105K4C This is one of the proteins that binds and probably mediates the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance (By similarity) Thewi_2240 ENOG4108R5J This protein binds to the 23S rRNA, and is important in its secondary structure. It is located near the subunit interface in the base of the L7 L12 stalk, and near the tRNA binding site of the peptidyltransferase center (By similarity) Thewi_2241 ENOG4108UJY One of the primary rRNA binding proteins, it binds directly to 16S rRNA central domain where it helps coordinate assembly of the platform of the 30S subunit (By similarity) Thewi_2242 ENOG41082QR Binds 16S rRNA, required for the assembly of 30S particles and may also be responsible for determining the conformation of the 16S rRNA at the A site (By similarity) Thewi_2243 ENOG4105CW6 This is 1 of the proteins that binds and probably mediates the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance. In the 70S ribosome it contacts protein S13 of the 30S subunit (bridge B1b), connecting the 2 subunits Thewi_2244 ENOG4105KAR One of the proteins that surrounds the polypeptide exit tunnel on the outside of the subunit (By similarity) Thewi_2245 ENOG4108UNN Binds to 23S rRNA. Forms part of two intersubunit bridges in the 70S ribosome (By similarity) Thewi_2246 ENOG4105K87 One of the primary rRNA binding proteins, it binds specifically to the 5'-end of 16S ribosomal Thewi_2247 ENOG41082SA 50s ribosomal protein l29 Thewi_2249 ENOG4105CKE Binds the lower part of the 30S subunit head. Binds mRNA in the 70S ribosome, positioning it for translation (By similarity) Thewi_2250 ENOG4105KAP The globular domain of the protein is located near the polypeptide exit tunnel on the outside of the subunit, while an extended beta-hairpin is found that lines the wall of the exit tunnel in the center of the 70S ribosome (By similarity) Thewi_2251 ENOG4105K7S Protein S19 forms a complex with S13 that binds strongly to the 16S ribosomal RNA (By similarity) Thewi_2252 ENOG4105CFD One of the primary rRNA binding proteins. Required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation. It has been suggested to have peptidyltransferase activity Thewi_2253 ENOG41080UE One of the early assembly proteins it binds 23S rRNA. One of the proteins that surrounds the polypeptide exit tunnel on the outside of the ribosome. Forms the main docking site for trigger factor binding to the ribosome (By similarity) Thewi_2254 ENOG4106U5A One of the primary rRNA binding proteins, this protein initially binds near the 5'-end of the 23S rRNA. It is important during the early stages of 50S assembly. It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome (By similarity) Thewi_2255 ENOG4105EEE One of the primary rRNA binding proteins, it binds directly near the 3'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit (By similarity) Thewi_2256 ENOG4108Z10 Involved in the binding of tRNA to the ribosomes (By similarity) Thewi_2257 ENOG4105CGV This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis (By similarity) Thewi_2258 ENOG4105CEJ Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome (By similarity) Thewi_2259 ENOG4108UHY One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the head domain of the 30S subunit. Is located at the subunit interface close to the decoding center, probably blocks exit of the E-site tRNA (By similarity) Thewi_2260 ENOG4108UKE Interacts with and stabilizes bases of the 16S rRNA that are involved in tRNA selection in the A site and with the mRNA backbone. Located at the interface of the 30S and 50S subunits, it traverses the body of the 30S subunit contacting proteins on the other side and probably holding the rRNA structure together. The combined cluster of proteins S8, S12 and S17 appears to hold together the shoulder and platform of the 30S subunit (By similarity) Thewi_2261 ENOG4105XPM ribosomal protein Thewi_2262 ENOG4105D27 DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates (By similarity) Thewi_2263 ENOG4108IIJ DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates (By similarity) Thewi_2264 ENOG4105KBC Seems to be the binding site for several of the factors involved in protein synthesis and appears to be essential for accurate translation (By similarity) Thewi_2266 ENOG4105C64 Binds directly to 23S rRNA. The L1 stalk is quite mobile in the ribosome, and is involved in E site tRNA release (By similarity) Thewi_2267 ENOG4108UIK This protein binds directly to 23S ribosomal RNA (By similarity) Thewi_2268 ENOG4105E5V Participates in transcription elongation, termination and antitermination (By similarity) Thewi_2269 ENOG410683Z Preprotein translocase SecE subunit Thewi_2270 ENOG4105WDJ 50S ribosomal protein L33 Thewi_2271 ENOG4105CGV This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis (By similarity) Thewi_2273 ENOG4105M0M Tetracycline resistance protein Thewi_2274 ENOG4105C2N RNA methyltransferase TrmH family group 3 Thewi_2276 ENOG4105C8N cysteinyl-tRNA synthetase Thewi_2277 ENOG4105D7W serine acetyltransferase Thewi_2280 ENOG4105C90 Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys- tRNA(Pro) is not edited by ProRS (By similarity) Thewi_2281 ENOG4108UH8 Involved in the biosynthesis of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP), two major building blocks of isoprenoid compounds. Catalyzes the conversion of 4- diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate (CDP-ME2P) to 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-CPP) with a corresponding release of cytidine 5-monophosphate (CMP) (By similarity) Thewi_2283 ENOG4105CE5 Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP) (By similarity) Thewi_2284 ENOG4105CTP PilT protein domain protein Thewi_2286 ENOG4105DNJ May play a role in the repair of endogenous alkylation damage (By similarity) Thewi_2287 ENOG4105C56 H( )-stimulated, divalent metal cation uptake system (By similarity) Thewi_2289 ENOG4105C2Z ATP-dependent CLP protease ATP-binding subunit Thewi_2290 ENOG41066A3 ATP guanido phosphotransferase Thewi_2291 ENOG4108Z0C Uvrb UvrC protein Thewi_2294 ENOG4105C6K Malic enzyme Thewi_2295 ENOG4105CEJ Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome (By similarity) Thewi_2296 ENOG4105BZ3 General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. Enzyme I transfers the phosphoryl group from phosphoenolpyruvate (PEP) to the phosphoryl carrier protein (HPr) (By similarity) Thewi_2297 ENOG4108YRF PTS System Thewi_2298 ENOG4105DM4 PTS System Thewi_2299 ENOG4105DM4 PTS System Thewi_2300 ENOG4105EMD TRANSCRIPTIONal Thewi_2301 ENOG4105BZU Histidine kinase Thewi_2305 ENOG410867B UPF0291 protein Thewi_2307 ENOG4105DRY peptidase s9 prolyl oligopeptidase active site domain protein Thewi_2309 ENOG4107ZIF 6-carboxy-5,6,7,8-tetrahydropterin synthase Thewi_2310 ENOG4105K8U 2-Amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase Thewi_2311 ENOG4105EEI dihydropteroate synthase Thewi_2312 ENOG4105EUJ GTP cyclohydrolase i Thewi_2314 ENOG4108UKH Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by cleavage factors such as GreA or GreB allows the resumption of elongation from the new 3'terminus. GreA releases sequences of 2 to 3 nucleotides (By similarity) Thewi_2320 ENOG4105E1K spore germination protein Thewi_2321 ENOG4105GEU Spore germination protein Thewi_2322 ENOG4105CGM Catalyzes the phosphorylation of pantothenate (Pan), the first step in CoA biosynthesis (By similarity) Thewi_2323 ENOG4105FH5 Membrane Thewi_2324 ENOG4105PV1 biotin lipoyl attachment domaiN-containing protein Thewi_2326 ENOG4107QX3 carboxylase Thewi_2327 ENOG4108ZVT methylmalonyl-coA epimerase Thewi_2329 ENOG4105D5P Methylmalonyl-coA mutase Thewi_2330 ENOG4105D5P Methylmalonyl-coA mutase Thewi_2333 ENOG4105BZU Histidine kinase Thewi_2335 ENOG4108UGV hypoxanthine phosphoribosyltransferase Thewi_2336 ENOG4105D3U Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine (By similarity) Thewi_2339 ENOG4105DDZ Methyltransferase required for the conversion of demethylmenaquinone (DMKH2) to menaquinone (MKH2) and the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2) (By similarity) Thewi_2340 ENOG4108K9K Uncharacterised protein family (UPF0236) Thewi_2341 ENOG4105SX7 Transporter Thewi_2345 ENOG4108T0W nitrous oxide maturation protein NosY Thewi_2349 ENOG4105CPQ Dehydrogenase Thewi_2350 ENOG4107EEK Catalyzes the decarboxylative condensation of pimeloyl- acyl-carrier protein and L-alanine to produce 8-amino-7- oxononanoate (AON), acyl-carrier protein , and carbon dioxide (By similarity) Thewi_2355 ENOG4105VG1 fes assembly suf system protein Thewi_2358 ENOG4107RUY (E1 component), alpha subunit Thewi_2359 ENOG4105CPP Dehydrogenase, E1 component Thewi_2367 ENOG4105BZU Histidine kinase Thewi_2383 ENOG41071TK Uncharacterised protein family (UPF0150) Thewi_2386 ENOG4107QJZ Glycosyl transferase, family 2 Thewi_2388 ENOG4105DPZ Two component transcriptional regulator (Winged helix family Thewi_2392 ENOG4105BZU Histidine kinase Thewi_2395 ENOG4105FUA antagonist Thewi_2396 ENOG4107MMM Anti-sigma-factor antagonist Thewi_2397 ENOG4105D5G Transposase Thewi_2419 ENOG4105DR5 peptidase Thewi_2434 ENOG4105DW3 Response regulator receiver modulated metal dependent phosphohydrolase Thewi_2450 ENOG4105D49 3-dehydroquinate synthase Thewi_2451 ENOG41060GV RNA binding s1 domain protein Thewi_2457 ENOG4105VYY sporulation protein yabp Thewi_2459 ENOG41082I2 s4 domain protein Thewi_2460 ENOG4105K70 DNA-binding protein Thewi_2461 ENOG4108IY2 MazG family Thewi_2463 ENOG4107SFI Major Facilitator superfamily Thewi_2464 ENOG4105C1H Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 (By similarity) Thewi_2465 ENOG4105BZU Histidine kinase Thewi_2466 ENOG4105CK6 Two component transcriptional regulator (Winged helix family Thewi_2467 ENOG4105MAX Superoxide reductase Thewi_2475 ENOG4105CB3 Required for the thiolation of cytidine in position 32 of tRNA, to form 2-thiocytidine (s(2)C32) (By similarity) Thewi_2476 ENOG4105CUZ uba thif-type nad fad binding protein Thewi_2484 ENOG4105EAH synthase Thewi_2491 ENOG4108YM7 Gcn5-related n-acetyltransferase Thewi_2495 ENOG4105D86 that it carries out the mismatch recognition step. This protein has a weak ATPase activity (By similarity) Thewi_2496 ENOG4105D86 that it carries out the mismatch recognition step. This protein has a weak ATPase activity (By similarity) Thewi_2500 ENOG4105EMN RNA polymerase sigma factor Thewi_2504 ENOG410811P TRANSCRIPTIONAl REGULATOR GntR family Thewi_2505 ENOG4105DPN Oligoendopeptidase f Thewi_2506 ENOG4105CH7 arginosuccinase Thewi_2509 ENOG4105C1M carbamoyl-phosphate synthetase glutamine chain Thewi_2510 ENOG4105C8Y Acetylornithine aminotransferase Thewi_2511 ENOG4105CAS nag kinase Thewi_2512 ENOG4105C5V Catalyzes two activities which are involved in the cyclic version of arginine biosynthesis the synthesis of N- acetylglutamate from glutamate and acetyl-CoA as the acetyl donor, and of ornithine by transacetylation between N(2)-acetylornithine and glutamate (By similarity) Thewi_2513 ENOG4105C0N N-acetylglutamate semialdehyde dehydrogenase Thewi_2516 ENOG4105Y5S NADH ubiquinone oxidoreductase, 24 kD Thewi_2519 ENOG4105CDJ oxidoreductase FAD NAD(P)-binding domain protein Thewi_2520 ENOG4105BZ8 glutamate synthase Thewi_2523 ENOG4105DCG PHP domain protein Thewi_2526 ENOG4105C52 Atpase, p-type (Transporting), had superfamily, subfamily ic Thewi_2527 ENOG4105YVM Phosphoesterase, PA-phosphatase related Thewi_2529 ENOG4105BZ1 (ABC) transporter Thewi_2532 ENOG4105DCG PHP domain protein Thewi_2535 ENOG4108EKI Dehydrogenase Thewi_2537 ENOG4105CPC Aldo Keto reductase Thewi_2540 ENOG4105DEF Extracellular solute-binding protein, family 5 Thewi_2542 ENOG4105C2T Binding-protein-dependent transport systems, inner membrane component Thewi_2543 ENOG4105C3U ABC transporter Thewi_2544 ENOG4108JQ7 (ABC) transporter Thewi_2549 ENOG4105CJ1 ABC, transporter Thewi_2551 ENOG4107WCI zn-dependent hydrolase of the beta-lactamase fold-like protein Thewi_2552 ENOG4105C75 arginyL-tRNA synthetase Thewi_2554 ENOG4105CPF Cell wall formation (By similarity) Thewi_2555 ENOG4105XEW small acid-soluble spore protein Thewi_2558 ENOG4108ZXQ stage ii sporulation protein r Thewi_2559 ENOG4108Q5U germination protein, ger(x)c family Thewi_2560 ENOG4108Q8B Spore germination protein Thewi_2564 ENOG4105DRR Transcriptional regulator, GntR family Thewi_2565 ENOG4105CTR Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol (By similarity) Thewi_2567 ENOG4105W42 Veg protein Thewi_2568 ENOG4105XFA sporulation peptidase YabG Thewi_2569 ENOG4108M14 stage v sporulation protein t Thewi_2570 ENOG4107UUG Plays a major role in protein secretion by helping the post-translocational extracellular folding of several secreted proteins (By similarity) Thewi_2571 ENOG4108JA2 transcriptioN-repair coupling factor Thewi_2574 ENOG4105C0H protease Thewi_2575 ENOG4105BZU Histidine kinase Thewi_2577 ENOG4105C5T Phosphoribosyl pyrophosphate synthase Thewi_2578 ENOG4105CAJ Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP- GlcNAc). The C-terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N-acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5- monophosphate (from uridine 5-triphosphate), a reaction catalyzed by the N-terminal domain (By similarity) Thewi_2579 ENOG4105K4W Could be involved in septation (By similarity) Thewi_2580 ENOG4105DFT pur operon repressor Thewi_2581 ENOG4105DFU Cell wall formation (By similarity) Thewi_2582 ENOG4105ETE Transcriptional regulator (LacI family Thewi_2584 ENOG4105VN1 Heat shock protein Thewi_2585 ENOG4105DM4 PTS System Thewi_2586 ENOG4105DM4 PTS System Thewi_2587 ENOG4105DE4 1-phosphofructokinase Thewi_2588 ENOG4105E98 transcriptional regulator DeoR family Thewi_2592 ENOG4107GBW cellulosome anchoring protein cohesin Thewi_2596 ENOG4105C8D Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen (By similarity) Thewi_2599 ENOG4105CY0 Metallo-Beta-Lactamase Thewi_2602 ENOG4105C5R Aminotransferase Thewi_2604 ENOG4105CN1 protease Thewi_2605 ENOG4105D5I Catalyzes the ATP-dependent phosphorylation of L- homoserine to L-homoserine phosphate (By similarity) Thewi_2606 ENOG4108IYI Catalyzes the gamma-elimination of phosphate from L- phosphohomoserine and the beta-addition of water to produce L- threonine (By similarity) Thewi_2608 ENOG41090I5 UPF0735 ACT domain-containing protein Thewi_2610 ENOG4105CUB amino acid Thewi_2611 ENOG4105Q2H domain protein Thewi_2617 ENOG4105ZHF CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Functions as a ssRNA-specific endoribonuclease (By similarity) Thewi_2618 ENOG4105DYN CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Acts as a dsDNA endonuclease. May be involved in the integration of spacer DNA into the CRISPR cassette (By similarity) Thewi_2620 ENOG4105EC3 peptidase S51, dipeptidase E Thewi_2629 ENOG4107YH3 SUF system FeS assembly protein, NifU family Thewi_2632 ENOG4105DIW FeS assembly protein SUFB Thewi_2633 ENOG4105C3K feS assembly ATPase SufC Thewi_2634 ENOG4105K5Y Binds to Cpn60 in the presence of Mg-ATP and suppresses the ATPase activity of the latter (By similarity) Thewi_2640 ENOG4105DA0 Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) (By similarity) Thewi_2642 ENOG4108UXA Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA (By similarity) Thewi_2645 ENOG4105CDF Cell wall formation. Adds enolpyruvyl to UDP-N- acetylglucosamine (By similarity) Thewi_2649 ENOG4105BZU Histidine kinase Thewi_2654 ENOG4105CI4 it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box) 5'-TTATC CA A CA A-3'. DnaA binds to ATP and to acidic phospholipids (By similarity) Thewi_2655 ENOG4105CD8 aminopeptidase N Thewi_2658 ENOG4105EGY DNA internalization-related competence protein ComEC Rec2 Thewi_2668 ENOG4105C91 Plays an important role in the de novo pathway of purine nucleotide biosynthesis Thewi_2669 ENOG4105DZ0 fad dependent oxidoreductase Thewi_2670 ENOG4105C8Q Methyl-accepting chemotaxis Thewi_2672 ENOG4108JYZ YitT family Thewi_2673 ENOG4107QMW pyridine nucleotide-disulfide oxidoreductase Thewi_2675 ENOG4105EKQ RNA-directed DNA polymerase Thewi_2677 ENOG4105DPN Oligoendopeptidase f Thewi_2678 ENOG4108D32 Methyltransferase Thewi_2679 ENOG4108IUW ABC transporter Thewi_2683 ENOG4105BZ1 (ABC) transporter Thewi_2691 ENOG4105BZ1 (ABC) transporter Thewi_2695 ENOG4105IJJ transcriptional regulator, crp fnr family Thewi_2696 ENOG4105CDU Replicative dna helicase Thewi_2702 ENOG4105VH8 Binds as a heterodimer with protein S6 to the central domain of the 16S rRNA, where it helps stabilize the platform of the 30S subunit (By similarity) Thewi_2703 ENOG4108UUM single-stranded DNA-binding protein Thewi_2704 ENOG4108ZDX Binds together with S18 to 16S ribosomal RNA (By similarity) Thewi_2705 ENOG4105VDK protein, conserved in bacteria Thewi_2706 ENOG4105D8X mechanosensitive ion channel Thewi_2707 ENOG4108ZDA Sporulation protein YyaC Thewi_2710 ENOG4105CBD Diacylglycerol kinase Thewi_2712 ENOG4105C9B Cysteine desulfurase Thewi_2713 ENOG4105FA0 parb-like partition protein Thewi_2714 ENOG4107QJE Chromosome Partitioning Protein Thewi_2715 ENOG410636K Effects nucleoid occlusion by binding relatively nonspecifically to DNA and preventing the assembly of the division machinery in the vicinity of the nucleoid, especially under conditions that disturb the cell cycle. It helps to coordinate cell division and chromosome segregation by preventing the formation of the Z ring through the nucleoid, which would cause chromosome breakage (By similarity) Thewi_2718 ENOG4107RE5 NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34 (By similarity) Thewi_2719 ENOG4105C1H Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 (By similarity) Thewi_2720 ENOG4108Z7S Single-stranded nucleic acid binding R3H domain-containing protein Thewi_2721 ENOG4107R4E Membrane Thewi_2722 ENOG4105VAS Could be involved in insertion of integral membrane proteins into the membrane (By similarity) Thewi_2723 ENOG4105M7T RNaseP catalyzes the removal of the 5'-leader sequence from pre-tRNA to produce the mature 5'-terminus. It can also cleave other RNA substrates such as 4.5S RNA. The protein component plays an auxiliary but essential role in vivo by binding to the 5'-leader sequence and broadening the substrate specificity of the ribozyme (By similarity)