locus-tag class description Spica_0001 ENOG4105CI4 it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box) 5'-TTATC CA A CA A-3'. DnaA binds to ATP and to acidic phospholipids (By similarity) Spica_0002 ENOG4105CZ8 DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped onto DNA, it slides freely (bidirectional and ATP- independent) along duplex DNA (By similarity) Spica_0003 ENOG4105C3X it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP (By similarity) Spica_0005 ENOG4105VG0 50S ribosomal protein l34 Spica_0006 ENOG4108541 RNaseP catalyzes the removal of the 5'-leader sequence from pre-tRNA to produce the mature 5'-terminus. It can also cleave other RNA substrates such as 4.5S RNA. The protein component plays an auxiliary but essential role in vivo by binding to the 5'-leader sequence and broadening the substrate specificity of the ribozyme (By similarity) Spica_0007 ENOG4105DHW Required for the insertion and or proper folding and or complex formation of integral membrane proteins into the membrane. Involved in integration of membrane proteins that insert both dependently and independently of the Sec translocase complex, as well as at least some lipoproteins Spica_0008 ENOG4108Z7S Single-stranded nucleic acid binding R3H domain-containing protein Spica_0010 ENOG4105I03 Flagellin Spica_0012 ENOG4105CU0 Catalyzes the 2'-O-methylation of the ribose of cytidine 1402 (C1402) in 16S rRNA (By similarity) Spica_0016 ENOG4108HU6 Metal dependent phosphohydrolase Spica_0017 ENOG4105DAN ATP-dependent protease Spica_0019 ENOG4107WTJ Methyl-accepting chemotaxis sensory transducer Spica_0021 ENOG4107XF7 NGG1p interacting factor 3 protein, NIF3 Spica_0022 ENOG410835P This protein specifically catalyzes the removal of signal peptides from prolipoproteins (By similarity) Spica_0030 ENOG4105E09 nadh pyrophosphatase Spica_0032 ENOG4105C2F Histidine kinase Spica_0033 ENOG4108NEV two component transcriptional regulator, LytTR family Spica_0034 ENOG4105DFW ABC transporter Spica_0035 ENOG4105DFW ABC transporter Spica_0037 ENOG4108HWW ABC transporter Spica_0038 ENOG4107QI1 Mate efflux family protein Spica_0040 ENOG4105DR5 peptidase Spica_0042 ENOG4105XVJ Protein of unknown function (DUF327) Spica_0043 ENOG4105MA4 psp1 domain protein Spica_0046 ENOG4107R2U Catalyzes the formation of dTMP and tetrahydrofolate from dUMP and methylenetetrahydrofolate (By similarity) Spica_0047 ENOG4105DW3 Response regulator receiver modulated metal dependent phosphohydrolase Spica_0048 ENOG4105C8Q Methyl-accepting chemotaxis Spica_0050 ENOG4107TDE lipolytic protein G-D-S-L family Spica_0055 ENOG4105ETE Transcriptional regulator (LacI family Spica_0058 ENOG4105M6F mutator MutT protein Spica_0062 ENOG4105DW3 Response regulator receiver modulated metal dependent phosphohydrolase Spica_0063 ENOG4107R2I The heterodimer acts as both an ATP-dependent DNA helicase and an ATP-dependent, dual-direction single-stranded exonuclease. Recognizes the chi site generating a DNA molecule suitable for the initiation of homologous recombination. The AddA nuclease domain is required for chi fragment generation Spica_0066 ENOG4105DMW Dehydrogenase reductase Spica_0067 ENOG4105E98 transcriptional regulator DeoR family Spica_0069 ENOG4108IUM Glycogen debranching enzyme Spica_0073 ENOG4105C0A alcohol dehydrogenase Spica_0074 ENOG4105EB3 Histidine kinase Spica_0083 ENOG4105DPZ Two component transcriptional regulator (Winged helix family Spica_0085 ENOG4108IUM Glycogen debranching enzyme Spica_0086 ENOG4105CG3 alpha amylase, catalytic region Spica_0088 ENOG4105EG2 ABC transporter, permease Spica_0094 ENOG4105U6W MutT NUDIX family protein Spica_0102 ENOG4107QMH DNA methylase Spica_0109 ENOG4105XMH SpoVT AbrB domain protein Spica_0116 ENOG4105BZU Histidine kinase Spica_0118 ENOG4105C65 Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF- independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism (By similarity) Spica_0119 ENOG41090KN UPF0303 protein Spica_0122 ENOG4105BZU Histidine kinase Spica_0123 ENOG4105WH1 Two component transcriptional regulator luxr family Spica_0125 ENOG4105E9V Periplasmic binding protein LacI transcriptional regulator Spica_0126 ENOG4105E9V Periplasmic binding protein LacI transcriptional regulator Spica_0127 ENOG4105C2J ABC transporter Spica_0128 ENOG4105CNN ABC, transporter Spica_0129 ENOG4107SQ3 mannitol-1-phosphate 5-dehydrogenase Spica_0132 ENOG4105VVB Spica_0134 ENOG4105CDJ oxidoreductase FAD NAD(P)-binding domain protein Spica_0135 ENOG4105BZ8 glutamate synthase Spica_0136 ENOG4105EYW Ion transport protein Spica_0137 ENOG4105CGH 5'-nucleotidase Spica_0142 ENOG4108YZY peptidylprolyl cis-trans isomerase Spica_0147 ENOG4105DRS transport system, permease Spica_0148 ENOG4105F0P binding-protein-dependent transport systems inner membrane Component Spica_0149 ENOG4108JP0 extracellular solute-binding protein family 1 Spica_0150 ENOG4105WH1 Two component transcriptional regulator luxr family Spica_0151 ENOG4105BZU Histidine kinase Spica_0152 ENOG4105DIW FeS assembly protein SUFB Spica_0156 ENOG4107TIA phenylalanyl-tRNA synthetase (beta subunit) Spica_0157 ENOG4105CSS phenylalanyl-tRNA synthetase (alpha subunit) Spica_0162 ENOG4105BZU Histidine kinase Spica_0163 ENOG4105CEH Catalyzes the synthesis of dihydrouridine a modified base found in the D-loop of most tRNAs (By similarity) Spica_0164 ENOG4105F8V Hydrolase, tatD family Spica_0166 ENOG4105D1W Converts 2,5-diamino-6-(ribosylamino)-4(3h)-pyrimidinone 5'-phosphate into 5-amino-6-(ribosylamino)-2,4(1h,3h)- pyrimidinedione 5'-phosphate (By similarity) Spica_0167 ENOG4108R6K riboflavin synthase, subunit alpha Spica_0168 ENOG4105C66 Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate (By similarity) Spica_0169 ENOG4108UTT Catalyzes the formation of 6,7-dimethyl-8- ribityllumazine by condensation of 5-amino-6-(D- ribitylamino)uracil with 3,4-dihydroxy-2-butanone 4-phosphate. This is the penultimate step in the biosynthesis of riboflavin (By similarity) Spica_0170 ENOG4105DWF phosphomethylpyrimidine kinase Spica_0171 ENOG4105C4U 4-methyl-5-beta-hydroxyethylthiazole kinase Spica_0173 ENOG4108VXP Specifically catalyzes the dephosphorylation of 2- phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stress (By similarity) Spica_0175 ENOG4105DDQ anthranilate synthase Spica_0177 ENOG4105CG0 The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine (By similarity) Spica_0178 ENOG4105F6H The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate (By similarity) Spica_0180 ENOG41083MR nifu domain-containing protein Spica_0181 ENOG41069P5 Protein slyX homolog Spica_0182 ENOG4105CTQ phosphohexokinase Spica_0185 ENOG4105BZU Histidine kinase Spica_0194 ENOG4105ECY inositol monophosphatase Spica_0197 ENOG4107XH4 PPIases accelerate the folding of proteins (By similarity) Spica_0198 ENOG4105BZU Histidine kinase Spica_0199 ENOG4108HMR Key enzyme in the regulation of glycerol uptake and metabolism (By similarity) Spica_0200 ENOG4106N2C Spica_0201 ENOG4105F3K May play a role in DNA repair. It seems to be involved in an RecBC-independent recombinational process of DNA repair. It may act with RecF and RecO (By similarity) Spica_0202 ENOG4105KDX Binds to DNA and alters its conformation. May be involved in regulation of gene expression, nucleoid organization and DNA protection (By similarity) Spica_0203 ENOG4107QMP dna polymerase iii subunits gamma and tau Spica_0210 ENOG4105DRS transport system, permease Spica_0211 ENOG4105S9S Binding-protein-dependent transport systems, inner membrane component Spica_0213 ENOG4105WH1 Two component transcriptional regulator luxr family Spica_0214 ENOG4105BZU Histidine kinase Spica_0222 ENOG4105K5B UPF0225 protein Spica_0223 ENOG4108IPJ Membrane Spica_0225 ENOG4105CDF Cell wall formation. Adds enolpyruvyl to UDP-N- acetylglucosamine (By similarity) Spica_0226 ENOG4105BZ5 ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins (By similarity) Spica_0229 ENOG4105DPZ Two component transcriptional regulator (Winged helix family Spica_0233 ENOG4105CHR reductase Spica_0239 ENOG4105CN1 protease Spica_0243 ENOG4107WGG Transfers the N-acyl diglyceride group on what will become the N-terminal cysteine of membrane lipoproteins (By similarity) Spica_0244 ENOG4105D98 threonine synthase Spica_0245 ENOG4105VN1 Heat shock protein Spica_0248 ENOG4105CV1 Transketolase (EC 2.2.1.1) Spica_0250 ENOG4105BZW Glycerate kinase Spica_0251 ENOG4107VHA regulatoR Spica_0252 ENOG4105CDV Ompa motb domain protein Spica_0253 ENOG4105CCX Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor (By similarity) Spica_0254 ENOG4105FDS Catalyzes the decarboxylation of S-adenosylmethionine to S-adenosylmethioninamine (dcAdoMet), the propylamine donor required for the synthesis of the polyamines spermine and spermidine from the diamine putrescine (By similarity) Spica_0257 ENOG410605W Major Facilitator Spica_0269 ENOG4105C9M aspartyl-trna synthetase Spica_0270 ENOG4105KT7 mgtC SapB transporter Spica_0274 ENOG4108CWB sugar kinase ygcE Spica_0275 ENOG4105C5T Phosphoribosyl pyrophosphate synthase Spica_0276 ENOG4107QKZ Ompa motb domain protein Spica_0279 ENOG4105XMH SpoVT AbrB domain protein Spica_0280 ENOG4105M0J Toxic component of a toxin-antitoxin (TA) module. A Spica_0286 ENOG4105G0K glycine radical enzyme YjjI family Spica_0287 ENOG41076CZ activating enzyme Spica_0294 ENOG4108IQE Amp-dependent synthetase and ligase Spica_0295 ENOG4105DW3 Response regulator receiver modulated metal dependent phosphohydrolase Spica_0296 ENOG4105BZU Histidine kinase Spica_0298 ENOG4105CJX Molecular chaperone. Has ATPase activity (By similarity) Spica_0302 ENOG4105BZ1 (ABC) transporter Spica_0305 ENOG4105E8R UPF0173 metal-dependent hydrolase Spica_0306 ENOG4105CHR reductase Spica_0308 ENOG4105WH1 Two component transcriptional regulator luxr family Spica_0310 ENOG4105XN3 extracellular solute-binding protein family 1 Spica_0311 ENOG4105F0P binding-protein-dependent transport systems inner membrane Component Spica_0312 ENOG4105DRS transport system, permease Spica_0319 ENOG4105F8J Adenylate Guanylate cyclase Spica_0321 ENOG4105C9B Cysteine desulfurase Spica_0322 ENOG4105JRK feS assembly protein SufD Spica_0324 ENOG4105C3K feS assembly ATPase SufC Spica_0326 ENOG4108X0B nad(p)h dehydrogenase (quinone) Spica_0327 ENOG4108TSD Specifically methylates the N3 position of the uracil ring of uridine 1498 (m3U1498) in 16S rRNA. Acts on the fully assembled 30S ribosomal subunit (By similarity) Spica_0331 ENOG4108IUN Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties (By similarity) Spica_0333 ENOG4105EMN RNA polymerase sigma factor Spica_0337 ENOG4107R5Y anaerobic glycerol-3-phosphate dehydrogenase, subunit A Spica_0340 ENOG4105C1N GTPase that associates with the 50S ribosomal subunit and may have a role during protein synthesis or ribosome biogenesis (By similarity) Spica_0341 ENOG4105E4C Lytic transglycosylase catalytic Spica_0347 ENOG4105EAH synthase Spica_0348 ENOG4105F7M Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit (By similarity) Spica_0353 ENOG4108JP0 extracellular solute-binding protein family 1 Spica_0354 ENOG4105S9S Binding-protein-dependent transport systems, inner membrane component Spica_0355 ENOG4105I1K ABC transporter Spica_0356 ENOG4105E2V Glycosyltransferase 36 Spica_0359 ENOG4105CE1 Ferrous iron transport protein b Spica_0360 ENOG4105EMU Dehydrogenase Spica_0361 ENOG4105CNU cell division protein FtsK Spica_0365 ENOG4107QSV RmuC domain protein Spica_0366 ENOG4105F7J membrane-bound serine protease Spica_0367 ENOG4105DI5 UPF0365 protein Spica_0372 ENOG41084ED 50S ribosomal protein L33 Spica_0374 ENOG4105E5V Participates in transcription elongation, termination and antitermination (By similarity) Spica_0375 ENOG4108UIK This protein binds directly to 23S ribosomal RNA (By similarity) Spica_0376 ENOG4105C64 Binds directly to 23S rRNA. The L1 stalk is quite mobile in the ribosome, and is involved in E site tRNA release (By similarity) Spica_0377 ENOG4108VZM 50s ribosomal protein L10 Spica_0378 ENOG4105KBC Seems to be the binding site for several of the factors involved in protein synthesis and appears to be essential for accurate translation (By similarity) Spica_0379 ENOG4108IIJ DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates (By similarity) Spica_0380 ENOG4105D27 DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates (By similarity) Spica_0381 ENOG4108UKE Interacts with and stabilizes bases of the 16S rRNA that are involved in tRNA selection in the A site and with the mRNA backbone. Located at the interface of the 30S and 50S subunits, it traverses the body of the 30S subunit contacting proteins on the other side and probably holding the rRNA structure together. The combined cluster of proteins S8, S12 and S17 appears to hold together the shoulder and platform of the 30S subunit (By similarity) Spica_0382 ENOG4108UHY One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the head domain of the 30S subunit. Is located at the subunit interface close to the decoding center, probably blocks exit of the E-site tRNA (By similarity) Spica_0383 ENOG4105CGV This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis (By similarity) Spica_0384 ENOG4108Z10 Involved in the binding of tRNA to the ribosomes (By similarity) Spica_0385 ENOG4105EEE One of the primary rRNA binding proteins, it binds directly near the 3'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit (By similarity) Spica_0386 ENOG4106U5A One of the primary rRNA binding proteins, this protein initially binds near the 5'-end of the 23S rRNA. It is important during the early stages of 50S assembly. It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome (By similarity) Spica_0387 ENOG41080UE One of the early assembly proteins it binds 23S rRNA. One of the proteins that surrounds the polypeptide exit tunnel on the outside of the ribosome. Forms the main docking site for trigger factor binding to the ribosome (By similarity) Spica_0388 ENOG4105CFD One of the primary rRNA binding proteins. Required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation. It has been suggested to have peptidyltransferase activity Spica_0389 ENOG4105K7S Protein S19 forms a complex with S13 that binds strongly to the 16S ribosomal RNA (By similarity) Spica_0390 ENOG41082G4 The globular domain of the protein is located near the polypeptide exit tunnel on the outside of the subunit, while an extended beta-hairpin is found that lines the wall of the exit tunnel in the center of the 70S ribosome (By similarity) Spica_0391 ENOG4105CKE Binds the lower part of the 30S subunit head. Binds mRNA in the 70S ribosome, positioning it for translation (By similarity) Spica_0392 ENOG4108R70 Binds 23S rRNA and is also seen to make contacts with the A and possibly P site tRNAs (By similarity) Spica_0393 ENOG41086S0 50s ribosomal protein l29 Spica_0394 ENOG4105K87 One of the primary rRNA binding proteins, it binds specifically to the 5'-end of 16S ribosomal Spica_0395 ENOG4108UNN Binds to 23S rRNA. Forms part of two intersubunit bridges in the 70S ribosome (By similarity) Spica_0396 ENOG4105KAR One of the proteins that surrounds the polypeptide exit tunnel on the outside of the subunit (By similarity) Spica_0397 ENOG4105CW6 This is 1 of the proteins that binds and probably mediates the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance. In the 70S ribosome it contacts protein S13 of the 30S subunit (bridge B1b), connecting the 2 subunits Spica_0398 ENOG41082QR Binds 16S rRNA, required for the assembly of 30S particles and may also be responsible for determining the conformation of the 16S rRNA at the A site (By similarity) Spica_0399 ENOG4108UJY One of the primary rRNA binding proteins, it binds directly to 16S rRNA central domain where it helps coordinate assembly of the platform of the 30S subunit (By similarity) Spica_0400 ENOG4108R5J This protein binds to the 23S rRNA, and is important in its secondary structure. It is located near the subunit interface in the base of the L7 L12 stalk, and near the tRNA binding site of the peptidyltransferase center (By similarity) Spica_0401 ENOG4105K4C This is one of the proteins that binds and probably mediates the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance (By similarity) Spica_0402 ENOG4108RA9 Located at the back of the 30S subunit body where it stabilizes the conformation of the head with respect to the body (By similarity) Spica_0404 ENOG4108UZ0 Binds to the 23S rRNA (By similarity) Spica_0405 ENOG4105CGG The central subunit of the protein translocation channel SecYEG. Consists of two halves formed by TMs 1-5 and 6-10. These two domains form a lateral gate at the front which open onto the bilayer between TMs 2 and 7, and are clamped together by SecE at the back. The channel is closed by both a pore ring composed of hydrophobic SecY resides and a short helix (helix 2A) on the extracellular side of the membrane which forms a plug. The plug probably moves laterally to allow the channel to open. The ring and the pore may move independently (By similarity) Spica_0406 ENOG41067V7 50S ribosomal protein L36 Spica_0407 ENOG4108Z04 Located at the top of the head of the 30S subunit, it contacts several helices of the 16S rRNA. In the 70S ribosome it contacts the 23S rRNA (bridge B1a) and protein L5 of the 50S subunit (bridge B1b), connecting the 2 subunits Spica_0408 ENOG4108UHH Located on the platform of the 30S subunit, it bridges several disparate RNA helices of the 16S rRNA. Forms part of the Shine-Dalgarno cleft in the 70S ribosome (By similarity) Spica_0409 ENOG4105G6W One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the body of the 30S subunit (By similarity) Spica_0410 ENOG4105CTF DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates (By similarity) Spica_0411 ENOG4108ZT0 50S ribosomal protein l17 Spica_0412 ENOG4106DC9 Spica_0413 ENOG4105CP9 extracellular ligand-binding receptor Spica_0414 ENOG4105C32 Amino acid ABC transporter (Permease) Spica_0415 ENOG4105CF8 branched-chain amino acid ABC transporter, permease Spica_0416 ENOG4105C0Z ABC transporter Spica_0417 ENOG4108IV0 ABC, transporter Spica_0418 ENOG4105CP4 Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate- limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth (By similarity) Spica_0419 ENOG4105CWR In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance (By similarity) Spica_0420 ENOG4105EQY Methylates the class 1 translation termination release factors RF1 PrfA and RF2 PrfB on the glutamine residue of the universally conserved GGQ motif (By similarity) Spica_0421 ENOG4105C8K Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA (By similarity) Spica_0422 ENOG4105EJG cellulose synthase Spica_0423 ENOG4105EKD Catalyzes the hydrolytic deamination of adenine to hypoxanthine. Plays an important role in the purine salvage pathway and in nitrogen catabolism (By similarity) Spica_0427 ENOG4105CJ9 Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions (By similarity) Spica_0429 ENOG4105EJQ L-serine dehydratase Spica_0431 ENOG4105E99 Stereospecific condensation of phosphoenolpyruvate (PEP) and D-erythrose-4-phosphate (E4P) giving rise to 3-deoxy-D- arabino-heptulosonate-7-phosphate (DAHP) (By similarity) Spica_0432 ENOG4108UMW Endonuclease that specifically degrades the RNA of RNA- DNA hybrids (By similarity) Spica_0433 ENOG4105VEM Conserved domain protein Spica_0439 ENOG4105CTD carboxy-peptidase Spica_0442 ENOG41081GK CDP-diacylglycerol-glycerol-3-phosphate 3-phosphatidyl-transferase Spica_0444 ENOG4107SBB 4-alpha-glucanotransferase (EC 2.4.1.25) Spica_0446 ENOG4105CEJ Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome (By similarity) Spica_0449 ENOG41083NY Catalyzes the phosphorylation of the 3'-hydroxyl group of dephosphocoenzyme A to form coenzyme A (By similarity) Spica_0450 ENOG4105C2M dna polymerase I Spica_0451 ENOG4106VWR Spica_0453 ENOG4105CRD Catalyzes the transfer of the gamma-phosphate of ATP to D-galactose to form alpha-D-galactose-1-phosphate (Gal-1-P) (By similarity) Spica_0454 ENOG4105F0Z galactose-1-phosphate uridylyltransferase Spica_0457 ENOG4105C3H Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins. Plays a role in the quality control of integral membrane proteins (By similarity) Spica_0459 ENOG4105HBR Rna-binding protein Spica_0462 ENOG4105CBM Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12 (By similarity) Spica_0467 ENOG4108UM5 This protein is one of the early assembly proteins of the 50S ribosomal subunit, although it is not seen to bind rRNA by itself. It is important during the early stages of 50S assembly (By similarity) Spica_0468 ENOG4108UJD 30S ribosomal protein S9 Spica_0469 ENOG410691R Modulates RecA activity (By similarity) Spica_0470 ENOG4105DW3 Response regulator receiver modulated metal dependent phosphohydrolase Spica_0473 ENOG4106762 Involved in the synthesis of autoinducer 2 (AI-2) which is secreted by bacteria and is used to communicate both the cell density and the metabolic potential of the environment. The regulation of gene expression in response to changes in cell density is called quorum sensing. Catalyzes the transformation of S-ribosylhomocysteine (RHC) to homocysteine (HC) and 4,5- dihydroxy-2,3-pentadione (DPD) (By similarity) Spica_0474 ENOG4105DUF Catalyzes the irreversible cleavage of the glycosidic bond in both 5'-methylthioadenosine (MTA) and S- adenosylhomocysteine (SAH AdoHcy) to adenine and the corresponding thioribose, 5'-methylthioribose and S-ribosylhomocysteine, respectively (By similarity) Spica_0481 ENOG41082I4 UPF0102 protein Spica_0482 ENOG4105CPI Metal Dependent Phosphohydrolase Spica_0485 ENOG4108YY1 This protein is located at the 30S-50S ribosomal subunit interface and may play a role in the structure and function of the aminoacyl-tRNA binding site (By similarity) Spica_0486 ENOG4105D6X Specifically methylates guanosine-37 in various tRNAs (By similarity) Spica_0487 ENOG4108ZR6 An accessory protein needed during the final step in the assembly of 30S ribosomal subunit, possibly for assembly of the head region. Probably interacts with S19. Essential for efficient processing of 16S rRNA. May be needed both before and after RbfA during the maturation of 16S rRNA. It has affinity for free ribosomal 30S subunits but not for 70S ribosomes (By similarity) Spica_0488 ENOG4105VB6 UPF0109 protein Spica_0489 ENOG4105K5M 30s ribosomal protein S16 Spica_0491 ENOG4105CSF NADPH-dependent glycerol-3-phosphate dehydrogenase Spica_0493 ENOG4108UGX Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate (By similarity) Spica_0494 ENOG4105DI7 Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs (By similarity) Spica_0495 ENOG4105C31 Tryptophanyl-tRNA synthetase Spica_0497 ENOG4105K95 (LipO)protein Spica_0498 ENOG4105DW3 Response regulator receiver modulated metal dependent phosphohydrolase Spica_0499 ENOG4105C0D helicase Spica_0508 ENOG4105CF4 DegT DnrJ EryC1 StrS aminotransferase Spica_0510 ENOG4105D2T synthase Spica_0511 ENOG4107SE4 UDP-N-acetylglucosamine 2-epimerase Spica_0521 ENOG4105C4K Nad synthetase Spica_0522 ENOG4108UJE IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to HisF for the synthesis of IGP and AICAR (By similarity) Spica_0524 ENOG4105C5E polysaccharide biosynthesis protein Spica_0526 ENOG4105EAG UDP-N-acetylglucosamine 2-epimerase Spica_0528 ENOG4108I19 Catalyzes the formation of dTDP-glucose, from dTTP and glucose 1-phosphate, as well as its pyrophosphorolysis (By similarity) Spica_0529 ENOG4108URP Dtdp-4-dehydrorhamnose 3,5-epimerase Spica_0530 ENOG4105DBZ Dtdp-4-dehydrorhamnose reductase Spica_0535 ENOG4105C1B dTDP-glucose 4-6-dehydratase Spica_0540 ENOG4105C2J ABC transporter Spica_0544 ENOG4105EAM Na Pi-cotransporter Spica_0545 ENOG4105C5X domain protein Spica_0547 ENOG41062BZ domain protein Spica_0549 ENOG4105C70 Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis (By similarity) Spica_0553 ENOG4105EIS 1,4-dihydroxy-2-naphthoate octaprenyltransferase Spica_0556 ENOG4105D78 K07001 NTE family protein Spica_0557 ENOG4105D7W serine acetyltransferase Spica_0559 ENOG4105CWT An essential GTPase that binds both GDP and GTP, with rapid nucleotide exchange. Plays a role in 16S rRNA processing and 30S ribosomal subunit biogenesis and possibly also in cell cycle regulation and energy metabolism (By similarity) Spica_0560 ENOG4105ECA phosphoserine phosphatase homoserine phosphotransferase bifunctional protein Spica_0561 ENOG4105CGR Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec) (By similarity) Spica_0565 ENOG4105CDB Required for chromosome condensation and partitioning (By similarity) Spica_0569 ENOG4105M0J Toxic component of a toxin-antitoxin (TA) module. A Spica_0570 ENOG4107Y9E nuclease Spica_0571 ENOG4105C5H ABC transporter Spica_0577 ENOG4105VN1 Heat shock protein Spica_0578 ENOG4105CSA Coproporphyrinogen iii oxidase Spica_0579 ENOG4105CMQ Chloride channel Spica_0580 ENOG4105CK2 sodium dicarboxylate symporter Spica_0582 ENOG41067SE Tetr family transcriptional regulator Spica_0583 ENOG4105C4H Specifically methylates the guanine in position 2445 (m2G2445) and the guanine in position 2069 (m7G2069) of 23S rRNA (By similarity) Spica_0584 ENOG4105EZ6 Catalyzes the 2'-O-methylation at nucleotide C2498 in 23S rRNA (By similarity) Spica_0588 ENOG4105EHG Permease protein Spica_0593 ENOG4105MJ6 selenium-dependent hydroxylase accessory protein YqeC Spica_0597 ENOG4105EK5 Glycoside hydrolase family 8 Spica_0599 ENOG4105C50 Binding-protein-dependent transport systems, inner membrane component Spica_0601 ENOG4105CHZ Catalyzes the dehydration of D-mannonate (By similarity) Spica_0602 ENOG4105D0H Alpha-glucuronidase (EC 3.2.1.139) Spica_0605 ENOG4105DMW Dehydrogenase reductase Spica_0609 ENOG4107R1Q Membrane protein FdrA Spica_0610 ENOG4107REC cytoplasmic protein Spica_0613 ENOG4105DRR Transcriptional regulator, GntR family Spica_0615 ENOG4108HR6 Dehydrogenase Spica_0617 ENOG4105C2W permease Spica_0621 ENOG4105EQ7 Binding-protein-dependent transport systems, inner membrane component Spica_0622 ENOG4105DRS transport system, permease Spica_0624 ENOG4105ETE Transcriptional regulator (LacI family Spica_0625 ENOG4108UHU Aldolase Spica_0627 ENOG4105D50 Catalyzes the isomerization of 5-dehydro-4-deoxy-D- glucuronate to 3-deoxy-D-glycero-2,5-hexodiulosonate (By similarity) Spica_0628 ENOG4105CHR reductase Spica_0629 ENOG4105J4Q Transcriptional regulator Spica_0630 ENOG4105D2H Uronic isomerase Spica_0632 ENOG41063TF transcriptional regulator (AraC Spica_0633 ENOG4105F5H Glycosyl Hydrolase Family 88 Spica_0636 ENOG4105C50 Binding-protein-dependent transport systems, inner membrane component Spica_0644 ENOG4105D6J arsenicaL-resistance protein Spica_0646 ENOG4107SVV electron transport complex, RnfABCDGE type, B subunit Spica_0647 ENOG4105DGN Electron transport complex Spica_0648 ENOG4105DZB Electron transport complex Spica_0649 ENOG4108UQQ Electron transport complex Spica_0650 ENOG4105D6A Electron transport complex Spica_0651 ENOG4107QTR Required for nitrogen fixation. May be part of a membrane complex functioning as an intermediate in the electron transport to nitrogenase (By similarity) Spica_0654 ENOG4108UMN azlc family Spica_0655 ENOG4105KMT branched-chain amino acid Spica_0656 ENOG4105C8Q Methyl-accepting chemotaxis Spica_0657 ENOG4108YXY alkyl hydroperoxide reductase Thiol specific antioxidant Mal allergen Spica_0658 ENOG4105CX6 glutaminyL-tRNA synthetase Spica_0660 ENOG4107RJF diphosphate--fructose-6-phosphate 1-phosphotransferase (EC 2.7.1.90) Spica_0663 ENOG4105C41 Catalyzes the reversible phosphorylation of UMP to UDP (By similarity) Spica_0664 ENOG4108KS2 transglutaminase domain protein Spica_0667 ENOG4105DN4 Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released (By similarity) Spica_0668 ENOG4105C8N cysteinyl-tRNA synthetase Spica_0669 ENOG4107QWW Cell wall formation (By similarity) Spica_0675 ENOG4105G3C Type iv pilus assembly Spica_0679 ENOG410826E Dna repair protein radc Spica_0680 ENOG41090A1 ferritin Spica_0682 ENOG4107EEK Catalyzes the decarboxylative condensation of pimeloyl- acyl-carrier protein and L-alanine to produce 8-amino-7- oxononanoate (AON), acyl-carrier protein , and carbon dioxide (By similarity) Spica_0683 ENOG4105VYW UPF0251 protein Spica_0684 ENOG4105C0H protease Spica_0692 ENOG4108JJB ABC transporter Spica_0693 ENOG4105NSK ABC superfamily ATP binding cassette transporter membrane protein Spica_0694 ENOG4105BZU Histidine kinase Spica_0697 ENOG4105BZ1 (ABC) transporter Spica_0706 ENOG4107U53 septum site-determining protein minD Spica_0708 ENOG4105D3R alkyl hydroperoxide reductase Spica_0709 ENOG4108JUA Transcriptional regulator Spica_0710 ENOG4105C6H Catalyzes the formation of acetyl phosphate from acetate and ATP. Can also catalyze the reverse reaction (By similarity) Spica_0715 ENOG4105DRS transport system, permease Spica_0716 ENOG4105CZZ Alpha-N-arabinofuranosidase (EC 3.2.1.55) Spica_0720 ENOG4108YXI Secondary thiamine-phosphate synthase enzyme Spica_0722 ENOG4105DPN Oligoendopeptidase f Spica_0728 ENOG4105ETE Transcriptional regulator (LacI family Spica_0733 ENOG4105DPZ Two component transcriptional regulator (Winged helix family Spica_0734 ENOG4105BZU Histidine kinase Spica_0735 ENOG4105ETE Transcriptional regulator (LacI family Spica_0737 ENOG4105F0P binding-protein-dependent transport systems inner membrane Component Spica_0738 ENOG4105DRS transport system, permease Spica_0739 ENOG4105CJQ hydrolase, family 31 Spica_0741 ENOG4105CPC Aldo Keto reductase Spica_0745 ENOG4105KBX Transcriptional regulator, TetR family Spica_0749 ENOG4105I76 (LipO)protein Spica_0750 ENOG4105C5H ABC transporter Spica_0751 ENOG4105CMR udp-glucose 4-epimerase Spica_0752 ENOG4105C9Y Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bacterial cell walls (By similarity) Spica_0753 ENOG4105C66 Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate (By similarity) Spica_0754 ENOG4105CK3 Lysine 2,3-aminomutase YodO family protein Spica_0755 ENOG4108ZEF Reversibly transfers an adenylyl group from ATP to 4'- phosphopantetheine, yielding dephospho-CoA (dPCoA) and pyrophosphate (By similarity) Spica_0756 ENOG4107TFB basic membrane Spica_0757 ENOG4108JQ9 ABC transporter Spica_0758 ENOG4105UIB (ABC) transporter Spica_0760 ENOG4105D3A purine nucleoside phosphorylase DeoD-type Spica_0762 ENOG4105G5W regulator Fur family Spica_0765 ENOG4107QK4 glycoside hydrolase, family 3 domain protein Spica_0766 ENOG4105BZU Histidine kinase Spica_0767 ENOG4105CK6 Two component transcriptional regulator (Winged helix family Spica_0768 ENOG4108UTW Plays a role in the regulation of phosphate uptake Spica_0769 ENOG4105C0P Mg chelatase subunit ChlI Spica_0770 ENOG4105DB7 PHP domain protein Spica_0771 ENOG4105D6T Catalyzes the deamination of 5-methylthioadenosine and S-adenosyl-L-homocysteine into 5-methylthioinosine and S-inosyl-L- homocysteine, respectively. Is also able to deaminate adenosine (By similarity) Spica_0773 ENOG4105IJJ transcriptional regulator, crp fnr family Spica_0775 ENOG4105EP6 Maltosyltransferase that uses maltose 1-phosphate (M1P) as the sugar donor to elongate linear or branched alpha-(1- 4)- glucans. Is involved in a branched alpha-glucan biosynthetic pathway from trehalose, together with TreS, Mak and GlgB (By similarity) Spica_0778 ENOG4109003 Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis (By similarity) Spica_0781 ENOG4105C75 arginyL-tRNA synthetase Spica_0782 ENOG4105KK9 This protein binds to 23S rRNA in the presence of protein L20 (By similarity) Spica_0784 ENOG4105K46 50S ribosomal protein l27 Spica_0785 ENOG4105C9R An essential GTPase which binds GTP, GDP and possibly (p)ppGpp with moderate affinity, with high nucleotide exchange rates and a fairly low GTP hydrolysis rate (By similarity). It may play a role in control of the cell cycle, stress response, ribosome biogenesis and in those bacteria that undergo differentiation, in morphogenesis control Spica_0786 ENOG4107X4V Catalyzes the reversible adenylation of nicotinate mononucleotide (NaMN) to nicotinic acid adenine dinucleotide (NaAD) (By similarity) Spica_0788 ENOG4107ZFZ Functions as a ribosomal silencing factor. Interacts with ribosomal protein L14 (rplN), blocking formation of intersubunit bridge B8. Prevents association of the 30S and 50S ribosomal subunits and the formation of functional ribosomes, thus repressing translation (By similarity) Spica_0790 ENOG4105CTR Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol (By similarity) Spica_0792 ENOG4107TVT This is one of the proteins that binds to the 5S RNA in the ribosome where it forms part of the central protuberance (By similarity) Spica_0793 ENOG4105D3U Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine (By similarity) Spica_0798 ENOG4105CBS Chea signal transduction histidine kinase Spica_0799 ENOG4105BZU Histidine kinase Spica_0800 ENOG4105CMP catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR (By similarity) Spica_0801 ENOG4105CK6 Two component transcriptional regulator (Winged helix family Spica_0802 ENOG4108V3W Chemotaxis protein, CheW Spica_0806 ENOG4105C4S Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- translocation proceeds from a post-translocation (POST) complex to a pre-translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP-dependent manner (By similarity) Spica_0807 ENOG4106SUR Protein of unknown function DUF115 Spica_0808 ENOG4105DBD Chaperone involved in the correct folding and assembly of outer membrane proteins. Recognizes specific patterns of aromatic residues and the orientation of their side chains, which are found more frequently in integral outer membrane proteins. May act in both early periplasmic and late outer membrane-associated steps of protein maturation (By similarity) Spica_0809 ENOG41086CX Inherit from COG: 40-residue yvtn family beta-propeller repeat protein Spica_0811 ENOG4105CPC Aldo Keto reductase Spica_0814 ENOG4105ETE Transcriptional regulator (LacI family Spica_0815 ENOG4105MRB Catalyzes the interconversion of beta-pyran and beta- furan forms of D-ribose (By similarity) Spica_0816 ENOG4105C2J ABC transporter Spica_0817 ENOG4105CNN ABC, transporter Spica_0818 ENOG4105E9V Periplasmic binding protein LacI transcriptional regulator Spica_0828 ENOG4105S9S Binding-protein-dependent transport systems, inner membrane component Spica_0829 ENOG4105EB1 binding-protein-dependent transport systems inner membrane Component Spica_0830 ENOG4105ETE Transcriptional regulator (LacI family Spica_0833 ENOG4108V1T peptidylprolyl cis-trans isomerase Spica_0834 ENOG4108UGX Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate (By similarity) Spica_0836 ENOG4105C32 Amino acid ABC transporter (Permease) Spica_0837 ENOG4105CF8 branched-chain amino acid ABC transporter, permease Spica_0838 ENOG4105C0Z ABC transporter Spica_0839 ENOG4108IV0 ABC, transporter Spica_0840 ENOG4105C2S Catalyzes the NADPH dependent reduction of L-gamma- glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate (By similarity) Spica_0841 ENOG4105CGT Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline (By similarity) Spica_0844 ENOG4105D17 glycoside hydrolase, family 3 domain protein Spica_0845 ENOG4105C3M thioredoxin reductase Spica_0846 ENOG4105C8Q Methyl-accepting chemotaxis Spica_0848 ENOG41062VP Spica_0849 ENOG4105DG1 Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released (By similarity) Spica_0851 ENOG4105CPM Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine (By similarity) Spica_0853 ENOG4105CEJ Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome (By similarity) Spica_0854 ENOG4105FA0 parb-like partition protein Spica_0855 ENOG4108JQC penicillin-binding protein 1A Spica_0861 ENOG4105C8Q Methyl-accepting chemotaxis Spica_0862 ENOG4105CJI Catalyzes the interconversion of 2-phosphoglycerate and 3-phosphoglycerate (By similarity) Spica_0864 ENOG4105DM4 PTS System Spica_0870 ENOG4105MKQ Monovalent cation H antiporter subunit C Spica_0871 ENOG4108JPZ Monovalent cation H antiporter subunit D Spica_0874 ENOG4105C3Q Glycosyl transferase, family 2 Spica_0875 ENOG4105CU6 carbamoyl-phosphate synthetase ammonia chain Spica_0876 ENOG4105C1M carbamoyl-phosphate synthetase glutamine chain Spica_0877 ENOG4108Z66 YbaK ebsC protein Spica_0878 ENOG4105CNW domain protein Spica_0879 ENOG4105BZU Histidine kinase Spica_0880 ENOG4107Z9R response regulator Spica_0884 ENOG4105C5V Catalyzes two activities which are involved in the cyclic version of arginine biosynthesis the synthesis of N- acetylglutamate from glutamate and acetyl-CoA as the acetyl donor, and of ornithine by transacetylation between N(2)-acetylornithine and glutamate (By similarity) Spica_0885 ENOG4105CAS nag kinase Spica_0886 ENOG4105C8Y Acetylornithine aminotransferase Spica_0887 ENOG4105CDH Citrulline--aspartate ligase Spica_0888 ENOG4105CH7 arginosuccinase Spica_0890 ENOG4105CPQ Dehydrogenase Spica_0891 ENOG4105C20 Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) (By similarity) Spica_0896 ENOG4105D9H Catalyzes the attachment of glycine to tRNA(Gly) (By similarity) Spica_0917 ENOG4105FCI Catalyzes a reversible aldol reaction between acetaldehyde and D-glyceraldehyde 3-phosphate to generate 2-deoxy- D-ribose 5-phosphate (By similarity) Spica_0918 ENOG4107TFB basic membrane Spica_0919 ENOG4108JQ9 ABC transporter Spica_0920 ENOG4105UIB (ABC) transporter Spica_0922 ENOG4105CIM Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction alanine is first activated by ATP to form Ala- AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain (By similarity) Spica_0924 ENOG4105M7K Involved in the transcription termination process (By similarity) Spica_0925 ENOG4106JWJ Spica_0926 ENOG4105K5Y Binds to Cpn60 in the presence of Mg-ATP and suppresses the ATPase activity of the latter (By similarity) Spica_0928 ENOG4105DG1 Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released (By similarity) Spica_0929 ENOG4105CU9 asparagine synthetase A Spica_0931 ENOG4105CD9 Involved in the production of pyridoxal phosphate, probably by incorporating ammonia into the pyridine ring (By similarity) Spica_0932 ENOG4108UHX Involved in the hydrolysis of glutamine to glutamate and ammonia. Channels an ammonia molecule to PdxS (By similarity) Spica_0934 ENOG4107R37 Glutamate dehydrogenase Spica_0937 ENOG4105XXY Thioredoxin Spica_0938 ENOG4108IMY Transcriptional regulator Spica_0939 ENOG4105CQI Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate (By similarity) Spica_0940 ENOG4105MQS Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate (By similarity) Spica_0941 ENOG4105D8G Major Facilitator superfamily Spica_0942 ENOG4108UQV Membrane Spica_0944 ENOG4108QAF Methyl-accepting chemotaxis sensory transducer Spica_0945 ENOG4108Z4W Probably deamidates glutamine residues to glutamate on methyl-accepting chemotaxis receptors (MCPs), playing an important role in chemotaxis (By similarity) Spica_0947 ENOG4105CFH Aspartokinase Spica_0948 ENOG4105DUK Catalyzes the conversion of 4-hydroxy- tetrahydrodipicolinate (HTPA) to tetrahydrodipicolinate (By similarity) Spica_0949 ENOG4105CDP Catalyzes the condensation of (S)-aspartate-beta- semialdehyde (S)-ASA and pyruvate to 4-hydroxy- tetrahydrodipicolinate (HTPA) (By similarity) Spica_0950 ENOG4107QK3 aspartate-semialdehyde dehydrogenase (EC 1.2.1.11 Spica_0952 ENOG4107QSS Involved in the synthesis of meso-diaminopimelate (m-DAP or DL-DAP), required for both lysine and peptidoglycan biosynthesis. Catalyzes the direct conversion of tetrahydrodipicolinate to LL-diaminopimelate, a reaction that requires three enzymes in E.coli (By similarity) Spica_0958 ENOG4105CBS Chea signal transduction histidine kinase Spica_0959 ENOG4105YFD chemotaxis, protein Spica_0961 ENOG4107Z9R response regulator Spica_0964 ENOG4105C5I Dehydrogenase Spica_0966 ENOG4105DRH Involved in peptide bond synthesis. Stimulates efficient translation and peptide-bond synthesis on native or reconstituted 70S ribosomes in vitro. Probably functions indirectly by altering the affinity of the ribosome for aminoacyl-tRNA, thus increasing their reactivity as acceptors for peptidyl transferase (By similarity) Spica_0968 ENOG4105C1W two component, sigma54 specific, transcriptional regulator, Fis family Spica_0969 ENOG4105VFE sira family Spica_0970 ENOG41066VF YeeE YedE family protein Spica_0971 ENOG4105CUC YeeE YedE family protein Spica_0975 ENOG4105CS2 ec 3.2.1.21 Spica_0977 ENOG4105CAP With EpmB is involved in the beta-lysylation step of the post-translational modification of translation elongation factor P (EF-P). Catalyzes the ATP-dependent activation of (R)-beta-lysine produced by EpmB, forming a lysyl-adenylate, from which the beta- lysyl moiety is then transferred to the epsilon-amino group of a conserved specific lysine residue in EF-P (By similarity) Spica_0978 ENOG4105DMZ Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) (By similarity) Spica_0983 ENOG4105CPF Cell wall formation (By similarity) Spica_0984 ENOG4107EEN Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan (By similarity) Spica_0986 ENOG4105NBF ammecr1 domain protein Spica_0988 ENOG4105C3P Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln) (By similarity) Spica_0989 ENOG4105CHT Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln) (By similarity) Spica_0990 ENOG4105C5S asparaginyl-tRNA synthetase Spica_0993 ENOG4105W3S hemerythrin-like, metal-binding protein Spica_1000 ENOG4105F8J Adenylate Guanylate cyclase Spica_1009 ENOG4105I82 acetyl xylan esterase Spica_1013 ENOG4107QYE Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation (By similarity) Spica_1015 ENOG4105C34 Pseudouridine synthase Spica_1017 ENOG41090QR Spica_1018 ENOG4105RH4 Glycine cleavage system transcriptional repressor Spica_1019 ENOG4107U4H Methyl-accepting chemotaxis Spica_1021 ENOG4105DE4 1-phosphofructokinase Spica_1026 ENOG4108K5P (LipO)protein Spica_1028 ENOG4108JDX DNA polymerase iii, epsilon subunit Spica_1031 ENOG4107QSA Oxaloacetate decarboxylase Spica_1032 ENOG4105D9Q decarboxylase (Beta subunit) Spica_1033 ENOG4108UK1 Also displays a weak uracil phosphoribosyltransferase activity which is not physiologically significant (By similarity) Spica_1037 ENOG4108UH2 Endonuclease that specifically degrades the RNA of RNA- DNA hybrids (By similarity) Spica_1039 ENOG4107QJF Catalyzes the conversion of glucosamine-6-phosphate to glucosamine-1-phosphate (By similarity) Spica_1040 ENOG4105CCJ Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s(2)U34 (By similarity) Spica_1044 ENOG4105KG3 cytidine deaminase Spica_1045 ENOG4107RM2 PTS IIA-like nitrogen-regulatory protein PtsN Spica_1049 ENOG4106ARS Spica_1050 ENOG4107RFW hydrolase family 57 Spica_1052 ENOG4106NKH Spica_1057 ENOG4105F66 Binding-protein-dependent transport systems inner membrane component Spica_1066 ENOG4105BZS acriflavin resistance protein Spica_1083 ENOG4105D5G Transposase Spica_1086 ENOG4105CB3 Required for the thiolation of cytidine in position 32 of tRNA, to form 2-thiocytidine (s(2)C32) (By similarity) Spica_1088 ENOG4105DCI Phosphohydrolase Spica_1089 ENOG4105CQI Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate (By similarity) Spica_1093 ENOG4105WN5 stage II sporulation protein Spica_1095 ENOG4105C5H ABC transporter Spica_1096 ENOG4108UT2 hemolysin iii Spica_1097 ENOG4105D6S protein involved in catabolism of external DNA Spica_1101 ENOG41068FD Spica_1102 ENOG4105DBV ornithine carbamoyltransferase Spica_1107 ENOG4108Z11 Negatively regulates transcription of bacterial ribonucleotide reductase nrd genes and operons by binding to NrdR- boxes (By similarity) Spica_1108 ENOG4107UU3 sucrose-phosphate synthase (EC 2.4.1.14) Spica_1110 ENOG4105CHN ATP-dependent specificity component of the Clp protease. It directs the protease to specific substrates. Can perform chaperone functions in the absence of ClpP (By similarity) Spica_1111 ENOG4105CCQ Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins (By similarity) Spica_1112 ENOG4105DEA Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation Spica_1116 ENOG4105I03 Flagellin Spica_1117 ENOG4105W5T Binds to the C-terminal region of flagellin, which is implicated in polymerization, and participates in the assembly of the flagellum (By similarity) Spica_1118 ENOG4105VM8 Could accelerate the degradation of some genes transcripts potentially through selective RNA binding (By similarity) Spica_1119 ENOG4105DW3 Response regulator receiver modulated metal dependent phosphohydrolase Spica_1120 ENOG4105CPM Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine (By similarity) Spica_1121 ENOG4105K8N protein family UPF0079, ATPase Spica_1124 ENOG4106SAP Spica_1126 ENOG41083S7 flagellar protein FlaG Spica_1128 ENOG4105I03 Flagellin Spica_1130 ENOG4105D1H recombination factor protein RarA Spica_1133 ENOG4105CN0 Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate (By similarity) Spica_1134 ENOG4105DZA Converts GTP to 7,8-dihydroneopterin triphosphate (By similarity) Spica_1136 ENOG4105CIW Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine (By similarity) Spica_1140 ENOG4105SFS phosphoesterase RecJ domain protein Spica_1142 ENOG41073RW Cytoplasmic filament protein A Spica_1143 ENOG4108SXE Spica_1144 ENOG4107QJD pyruvate phosphate dikinase Spica_1147 ENOG4108IJ2 phosphate acetyltransferase Spica_1151 ENOG4105CIW Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine (By similarity) Spica_1154 ENOG4108UVJ appr-1-p processing domain protein Spica_1155 ENOG4105VZ9 UPF0235 protein Spica_1159 ENOG4105C5X domain protein Spica_1162 ENOG4105F03 Provides the (R)-glutamate required for cell wall biosynthesis (By similarity) Spica_1163 ENOG4105E06 May play a role in 30S ribosomal subunit biogenesis. Unusual circulary permuted GTPase that catalyzes rapid hydrolysis of GTP with a slow catalytic turnover (By similarity) Spica_1164 ENOG4105C98 muts2 protein Spica_1167 ENOG4105XBY Regulates arginine biosynthesis genes (By similarity) Spica_1168 ENOG4107QP8 phosphohexose isomerase Spica_1169 ENOG4105C8Q Methyl-accepting chemotaxis Spica_1171 ENOG4105F4C Redox regulated molecular chaperone. Protects both thermally unfolding and oxidatively damaged proteins from irreversible aggregation. Plays an important role in the bacterial defense system toward oxidative stress (By similarity) Spica_1177 ENOG4105F4G Membrane Spica_1178 ENOG4105VUC UPF0237 protein Spica_1179 ENOG4105CAG UPF0210 protein Spica_1181 ENOG4105C07 amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) (By similarity) Spica_1188 ENOG4107QXP Has an important function as a repair enzyme for proteins that have been inactivated by oxidation. Catalyzes the reversible oxidation-reduction of methionine sulfoxide in proteins to methionine (By similarity) Spica_1190 ENOG4107GFK fumarate Spica_1192 ENOG4105BZU Histidine kinase Spica_1193 ENOG4105C1W two component, sigma54 specific, transcriptional regulator, Fis family Spica_1195 ENOG4105EPW hflc protein Spica_1196 ENOG4107RIZ hflk protein Spica_1197 ENOG41068UM RNA Polymerase Spica_1198 ENOG4105DKX Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A) (By similarity) Spica_1199 ENOG4105C2V Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP) (By similarity) Spica_1200 ENOG4105EEI dihydropteroate synthase Spica_1201 ENOG4105C8B TIGR00159 family Spica_1202 ENOG4105TKT ybbr family Spica_1203 ENOG41083V3 Transfers the 4'-phosphopantetheine moiety from coenzyme A to a Ser of acyl-carrier-protein (By similarity) Spica_1205 ENOG4105DI7 Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs (By similarity) Spica_1206 ENOG4105JKQ Part of the ABC transporter complex BtuCDF involved in vitamin B12 import. Binds vitamin B12 and delivers it to the periplasmic surface of BtuC (By similarity) Spica_1207 ENOG4105EHG Permease protein Spica_1208 ENOG4105E0A ABC, transporter Spica_1210 ENOG4105C5R Aminotransferase Spica_1216 ENOG4105CHM Aminotransferase Spica_1217 ENOG4107YZD Protein of unknown function DUF115 Spica_1218 ENOG4105CJN penicillin-binding protein Spica_1220 ENOG4105DR5 peptidase Spica_1222 ENOG4108IAB Phoh family Spica_1225 ENOG4105C5U The UvrABC repair system catalyzes the recognition and processing of DNA lesions. UvrA is an ATPase and a DNA-binding protein. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. When the presence of a lesion has been verified by UvrB, the UvrA molecules dissociate (By similarity) Spica_1226 ENOG4105E7H involved in the assembly of LPS in the outer leaflet of the outer membrane. Determines N-hexane tolerance and is involved in outer membrane permeability. Essential for envelope biogenesis (By similarity) Spica_1228 ENOG4105CTJ Metal Dependent Phosphohydrolase Spica_1229 ENOG41082QW Single strand-specific metallo-endoribonuclease involved in late-stage 70S ribosome quality control and in maturation of the 3' terminus of the 16S rRNA (By similarity) Spica_1230 ENOG4105C37 (CBS) domain Spica_1236 ENOG4105CCP Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Acts as a receptor for the complex formed by the signal recognition particle (SRP) and the ribosome-nascent chain (RNC) Spica_1238 ENOG4105D60 lipoprotein releasing system transmembrane protein Spica_1239 ENOG4105CQU Part of the ABC transporter complex LolCDE involved in the translocation of mature outer membrane-directed lipoproteins, from the inner membrane to the periplasmic chaperone, LolA. Responsible for the formation of the LolA-lipoprotein complex in an ATP-dependent manner (By similarity) Spica_1242 ENOG41066A3 ATP guanido phosphotransferase Spica_1244 ENOG4105ERH Fumarylacetoacetate hydrolase Spica_1246 ENOG4108V06 Glutathione peroxidase Spica_1247 ENOG4105K56 Transcriptional regulator Spica_1248 ENOG4107QQ4 Catalyzes the synthesis of ADP-glucose, a sugar donor used in elongation reactions on alpha-glucans (By similarity) Spica_1249 ENOG4108I5C dEAD DEAH box helicase Spica_1250 ENOG4107X2U Inherit from COG: Exonuclease-like protein Spica_1253 ENOG4107R3E Citrate transporter Spica_1254 ENOG4108V82 Pyrophosphatase that hydrolyzes non-canonical purine nucleotides such as XTP and ITP dITP to their respective monophosphate derivatives. Might exclude non-canonical purines from DNA precursor pool, thus preventing their incorporation into DNA and avoiding chromosomal lesions (By similarity) Spica_1259 ENOG4105BZF Glycosyl transferase (Group 1 Spica_1260 ENOG4105CK7 Sodium proline symporter Spica_1264 ENOG4105DZS Phenylacetate-CoA ligase Spica_1266 ENOG4105CXW Synthesizes alpha-1,4-glucan chains using ADP-glucose (By similarity) Spica_1269 ENOG4105BZT Catalyzes the formation of 5-methyl-uridine at position 1939 (m5U1939) in 23S rRNA (By similarity) Spica_1270 ENOG4105E5G Alpha beta hydrolase fold Spica_1271 ENOG4105DJ0 Poly(A) Polymerase Spica_1274 ENOG4105EI2 Pseudouridine synthase Spica_1278 ENOG4105D6A Electron transport complex Spica_1281 ENOG4105DGN Electron transport complex Spica_1283 ENOG4105CKH Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation (By similarity) Spica_1285 ENOG4105IES Zinc phosphodiesterase, which displays some tRNA 3'- processing endonuclease activity. Probably involved in tRNA maturation, by removing a 3'-trailer from precursor tRNA (By similarity) Spica_1286 ENOG4105DA5 (ABC) transporter Spica_1295 ENOG410698I Spica_1298 ENOG4108UH4 Binds specifically to the SsrA RNA (tmRNA) and is required for stable association of SsrA with ribosomes (By similarity) Spica_1300 ENOG4105C3F Signal peptidase i Spica_1302 ENOG4105CSA Coproporphyrinogen iii oxidase Spica_1303 ENOG4105CQ7 ATP-dependent helicase Spica_1304 ENOG4108HRN Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate (By similarity) Spica_1307 ENOG4105D7T ABC transporter Spica_1309 ENOG4108JJB ABC transporter Spica_1310 ENOG4105VKD bioY protein Spica_1311 ENOG4105HJX Biotin- acetyl-CoA-carboxylase ligase Spica_1312 ENOG4107R05 Transfers the fatty acyl group on membrane lipoproteins (By similarity) Spica_1313 ENOG41080VC Galactokinase (EC 2.7.1.6) Spica_1316 ENOG4105NGQ Thioredoxin Spica_1317 ENOG4105D8I Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS (By similarity) Spica_1318 ENOG4107QI6 Cysteine desulfurase Spica_1320 ENOG4105CFH Aspartokinase Spica_1321 ENOG4105CHM Aminotransferase Spica_1323 ENOG4105ESV Glycosyl transferase, family 4 Spica_1326 ENOG4108URP Dtdp-4-dehydrorhamnose 3,5-epimerase Spica_1327 ENOG4105DBZ Dtdp-4-dehydrorhamnose reductase Spica_1328 ENOG4105C1B dTDP-glucose 4-6-dehydratase Spica_1333 ENOG4107STF Involved in phosphonate degradation (By similarity) Spica_1334 ENOG4105CUT histidyl-tRNA synthetase Spica_1339 ENOG4105CDY transcriptional regulatory protein Spica_1340 ENOG4105NHU Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group (By similarity) Spica_1341 ENOG4105KA4 The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing. RuvA stimulates, in the presence of DNA, the weak ATPase activity of RuvB (By similarity) Spica_1342 ENOG4105CKJ The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing (By similarity) Spica_1343 ENOG4105E2N Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA) (By similarity) Spica_1345 ENOG410805Y Phosphorylation of dTMP to form dTDP in both de novo and salvage pathways of dTTP synthesis (By similarity) Spica_1347 ENOG4105E1Z Part of the outer membrane protein assembly complex, which is involved in assembly and insertion of beta-barrel proteins into the outer membrane Spica_1350 ENOG4105D86 that it carries out the mismatch recognition step. This protein has a weak ATPase activity (By similarity) Spica_1351 ENOG4108UK1 Also displays a weak uracil phosphoribosyltransferase activity which is not physiologically significant (By similarity) Spica_1352 ENOG4105K7D Required for maturation of 30S ribosomal subunits (By similarity) Spica_1353 ENOG4105CHV Transcription elongation factor NusA Spica_1354 ENOG4107QHU One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex (By similarity) Spica_1355 ENOG41080JB Associates with free 30S ribosomal subunits (but not with 30S subunits that are part of 70S ribosomes or polysomes). Essential for efficient processing of 16S rRNA. May interact with the 5'-terminal helix region of 16S rRNA (By similarity) Spica_1356 ENOG4105D0T Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs (By similarity) Spica_1357 ENOG4105K77 Forms an intersubunit bridge (bridge B4) with the 23S rRNA of the 50S subunit in the ribosome (By similarity) Spica_1358 ENOG4105C62 Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction (By similarity) Spica_1359 ENOG4105C1V Peptidase, M16 Spica_1362 ENOG4105C6U Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis- dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) (By similarity) Spica_1367 ENOG4105DJV ribulose-phosphate 3-epimerase Spica_1371 ENOG4108YYA Hydrolyzes D-tyrosyl-tRNA(Tyr) into D-tyrosine and free tRNA(Tyr). Could be a defense mechanism against a harmful effect of D-tyrosine (By similarity) Spica_1372 ENOG4105C5T Phosphoribosyl pyrophosphate synthase Spica_1373 ENOG4105C68 Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with LexA causing its activation and leading to its autocatalytic cleavage (By similarity) Spica_1376 ENOG4105K8I Participates in chromosomal partition during cell division. May act via the formation of a condensin-like complex containing Smc and ScpA that pull DNA away from mid-cell into both cell halves (By similarity) Spica_1377 ENOG4105FE9 pseudouridine synthase Spica_1378 ENOG4105CAT Cytidine monophosphate kinase Spica_1379 ENOG4105CAV thus facilitating recognition of the initiation point. It is needed to translate mRNA with a short Shine-Dalgarno (SD) purine-rich sequence (By similarity) Spica_1380 ENOG4108JUA Transcriptional regulator Spica_1382 ENOG4105F8J Adenylate Guanylate cyclase Spica_1385 ENOG4105CNI cell division protein FtsW Spica_1386 ENOG4105CJN penicillin-binding protein Spica_1388 ENOG4105N3I Involved in formation and maintenance of cell shape (By similarity) Spica_1389 ENOG4105DFF Rod shape-determining protein mreb Spica_1392 ENOG4107QVY Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released (By similarity) Spica_1393 ENOG4105C9G DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis (By similarity) Spica_1394 ENOG4105HHA aminodeoxychorismate lyase Spica_1395 ENOG4105EY9 peptidase S8 and S53, subtilisin, kexin, sedolisin Spica_1397 ENOG4105IES Zinc phosphodiesterase, which displays some tRNA 3'- processing endonuclease activity. Probably involved in tRNA maturation, by removing a 3'-trailer from precursor tRNA (By similarity) Spica_1404 ENOG4108ZBM Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Also processes some mRNAs, and tRNAs when they are encoded in the rRNA operon (By similarity) Spica_1405 ENOG41084E5 Carrier of the growing fatty acid chain in fatty acid biosynthesis (By similarity) Spica_1406 ENOG41085YX 50S ribosomal protein L32 Spica_1407 ENOG4108ZEF Reversibly transfers an adenylyl group from ATP to 4'- phosphopantetheine, yielding dephospho-CoA (dPCoA) and pyrophosphate (By similarity) Spica_1409 ENOG4105SXG Spica_1412 ENOG4105C0B DNA polymerase III (alpha subunit) Spica_1414 ENOG4108Z03 MAF-like protein Spica_1415 ENOG4105CE9 30S ribosomal protein S2 Spica_1416 ENOG4105CU7 Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome (By similarity) Spica_1417 ENOG4108VCV Responsible for the release of ribosomes from messenger RNA at the termination of protein biosynthesis. May increase the efficiency of translation by recycling ribosomes from one round of translation to another (By similarity) Spica_1418 ENOG4105CR3 Catalyzes the condensation of isopentenyl diphosphate (IPP) with allylic pyrophosphates generating different type of terpenoids (By similarity) Spica_1419 ENOG4105KNE Phosphatidate cytidylyltransferase Spica_1420 ENOG4105CEA Catalyzes the NADP-dependent rearrangement and reduction of 1-deoxy-D-xylulose-5-phosphate (DXP) to 2-C-methyl-D-erythritol 4-phosphate (MEP) (By similarity) Spica_1421 ENOG4105DZP Membrane-associated zinc metalloprotease Spica_1424 ENOG4105CDU Replicative dna helicase Spica_1425 ENOG4105K9Q Binds to the 23S rRNA (By similarity) Spica_1427 ENOG4105VH8 Binds as a heterodimer with protein S6 to the central domain of the 16S rRNA, where it helps stabilize the platform of the 30S subunit (By similarity) Spica_1428 ENOG4105Z8H single-stranded DNA-binding protein Spica_1429 ENOG41084AY Binds together with S18 to 16S ribosomal RNA (By similarity) Spica_1432 ENOG4107S47 Specifically methylates the adenine in position 37 of tRNA(1)(Val) (anticodon cmo5UAC) (By similarity) Spica_1433 ENOG4105ZFX Spica_1435 ENOG4105C55 Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs Spica_1436 ENOG4106MP8 UPF0754 membrane protein Spica_1438 ENOG4105M6F mutator MutT protein Spica_1441 ENOG4105DR1 Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration (By similarity) Spica_1443 ENOG4105CI6 Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. Has a central role in coupling the hydrolysis of ATP to the transfer of proteins into and across the cell membrane, serving Spica_1448 ENOG4105CF4 DegT DnrJ EryC1 StrS aminotransferase Spica_1453 ENOG4105D49 3-dehydroquinate synthase Spica_1455 ENOG4105C6Y Udp-glucose 6-dehydrogenase Spica_1456 ENOG4105BZ1 (ABC) transporter Spica_1461 ENOG4105KPE licD family Spica_1470 ENOG4105D3Q Sugar transferase Spica_1473 ENOG4105CGA flagellar basal-body rod protein FlgG Spica_1474 ENOG4105CGA flagellar basal-body rod protein FlgG Spica_1476 ENOG4105CVG Homoserine O-transsuccinylase Spica_1477 ENOG4105CQ7 ATP-dependent helicase Spica_1480 ENOG4105C77 DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA (By similarity) Spica_1481 ENOG4107W7Y Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA (By similarity) Spica_1486 ENOG41090Q2 Spica_1495 ENOG4107V28 uracil phosphoribosyltransferase Spica_1498 ENOG4105C4E Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate (By similarity) Spica_1501 ENOG4108WBK V-type ATP synthase subunit D Spica_1502 ENOG4107RQX Produces ATP from ADP in the presence of a proton gradient across the membrane. The V-type beta chain is a regulatory subunit Spica_1503 ENOG4107RVI Produces ATP from ADP in the presence of a proton gradient across the membrane. The V-type alpha chain is a catalytic subunit (By similarity) Spica_1505 ENOG4108RY1 ATP synthase, subunit E Spica_1507 ENOG4105ENN converts alpha-aldose to the beta-anomer. It is active on D-glucose, L-arabinose, D-xylose, D-galactose, maltose and lactose (By similarity) Spica_1509 ENOG4105C1W two component, sigma54 specific, transcriptional regulator, Fis family Spica_1510 ENOG4105BZU Histidine kinase Spica_1512 ENOG4107JR8 Colicin v production protein Spica_1513 ENOG4105DVQ Cell wall formation. Catalyzes the transfer of a GlcNAc subunit on undecaprenyl-pyrophosphoryl-MurNAc-pentapeptide (lipid intermediate I) to form undecaprenyl-pyrophosphoryl-MurNAc- (pentapeptide)GlcNAc (lipid intermediate II) (By similarity) Spica_1517 ENOG4108V3W Chemotaxis protein, CheW Spica_1521 ENOG4105DN4 Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released (By similarity) Spica_1524 ENOG4105FZW flagellar biosynthesis regulator FlhF Spica_1525 ENOG4105D7B Flagellar biosynthesis protein flha Spica_1526 ENOG4105D00 flagellar biosynthetic protein flhB Spica_1527 ENOG4105D19 Role in flagellar biosynthesis (By similarity) Spica_1528 ENOG4105VS8 Flagellar biosynthetic protein fliq Spica_1529 ENOG4105CN7 Flagellar biosynthetic protein FliP Spica_1531 ENOG4105DD5 Flagellar motor switch protein Spica_1532 ENOG4105D24 FliM is one of three proteins (FliG, FliN, FliM) that forms the rotor-mounted switch complex (C ring), located at the base of the basal body. This complex interacts with the CheY and CheZ chemotaxis proteins, in addition to contacting components of the motor that determine the direction of flagellar rotation (By similarity) Spica_1533 ENOG41064SM flagellar basal body-associated protein flil Spica_1534 ENOG4107XSU flagellar motor protein motb Spica_1535 ENOG4107R0U MotA TolQ exbB proton channel Spica_1536 ENOG41068WV flagellar FlbD family protein Spica_1537 ENOG4105BZE flagellar hook protein, FlgE Spica_1538 ENOG410822I Flagellar hook capping protein Spica_1543 ENOG4105DD7 kinase (PfkB family Spica_1553 ENOG4108UN4 phage SPO1 DNA polymerase-related protein Spica_1554 ENOG4105C25 Primosomal protein n' Spica_1557 ENOG41080S5 HTH_DTXR Spica_1558 ENOG4108YZT Adenosylcobinamide kinase Spica_1561 ENOG4108UHF Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin) (By similarity) Spica_1562 ENOG4105CYJ ribosomal RNA small subunit methyltransferase B Spica_1563 ENOG4107QW4 alcohol dehydrogenase Spica_1574 ENOG4105HEE Binding Domain protein Spica_1577 ENOG4108Z4J Cytidine monophosphate kinase Spica_1578 ENOG4105DSJ YicC domain protein Spica_1579 ENOG4105DFU Cell wall formation (By similarity) Spica_1580 ENOG4105DPM folylpolyglutamate synthase Dihydrofolate synthase Spica_1582 ENOG4105C34 Pseudouridine synthase Spica_1585 ENOG4105DFA alanine racemase domain protein Spica_1586 ENOG4105D1X Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits (By similarity) Spica_1591 ENOG4108IFH alkyl hydroperoxide reductase Thiol specific antioxidant Mal allergen Spica_1592 ENOG4107QIV Cytochrome c biogenesis protein Spica_1595 ENOG4105DP5 Catalyzes the biosynthesis of agmatine from arginine (By similarity) Spica_1596 ENOG4105CE8 Transfers the fatty acyl group on membrane lipoproteins (By similarity) Spica_1600 ENOG4105FF6 Modulates transcription in response to changes in cellular NADH NAD( ) redox state (By similarity) Spica_1601 ENOG4105DR4 Methyltransferase Spica_1603 ENOG41082DI 50s ribosomal protein l31 Spica_1604 ENOG4105C4P Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template (By similarity) Spica_1605 ENOG4105CEV competence damage-inducible protein Spica_1609 ENOG4107RI4 Transfers the fatty acyl group on membrane lipoproteins (By similarity) Spica_1611 ENOG4105KQV Binds directly to 16S ribosomal RNA (By similarity) Spica_1613 ENOG4108ZF1 Transcriptional regulator, BadM Rrf2 family Spica_1615 ENOG4105DVT helicase Spica_1617 ENOG4105E9Q Acyl-transferase Spica_1618 ENOG4108JA2 transcriptioN-repair coupling factor Spica_1620 ENOG4106UUF Spica_1623 ENOG4105DF5 DNA topoisomerase IV, subunit A Spica_1624 ENOG4105DNX Dna topoisomerase iv (Subunit b) Spica_1626 ENOG4105CEJ Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome (By similarity) Spica_1628 ENOG4105C5S asparaginyl-tRNA synthetase Spica_1629 ENOG4105DZ1 carboxypeptidase Spica_1630 ENOG4107NGC Flagellar filament outer layer protein Spica_1632 ENOG4108KNB n-acetylmuramoyl-l-alanine amidase Spica_1635 ENOG4107TY6 Acylneuraminate cytidylyltransferase Spica_1637 ENOG4108ZVA radical SAM domain protein Spica_1638 ENOG4105DVS Bidirectionally degrades single-stranded DNA into large acid-insoluble oligonucleotides, which are then degraded further into small acid-soluble oligonucleotides (By similarity) Spica_1639 ENOG410868T Bidirectionally degrades single-stranded DNA into large acid-insoluble oligonucleotides, which are then degraded further into small acid-soluble oligonucleotides (By similarity) Spica_1640 ENOG4105DC6 This protein is involved in the repair of mismatches in DNA. It is required for dam-dependent methyl-directed DNA mismatch repair. May act as a molecular matchmaker , a protein that promotes the formation of a stable complex between two or more DNA-binding proteins in an ATP-dependent manner without itself being part of a final effector complex (By similarity) Spica_1643 ENOG4105F8V Hydrolase, tatD family Spica_1647 ENOG4108YZA Nitrogen regulatory protein pii Spica_1649 ENOG4108JUA Transcriptional regulator Spica_1650 ENOG4105D22 carbon starvation protein Spica_1652 ENOG4105D0U Arsenite-activated ATPase (ArsA) Spica_1655 ENOG410811P TRANSCRIPTIONAl REGULATOR GntR family Spica_1656 ENOG4107WFU Glutamate mutase subunit sigma Spica_1658 ENOG4105DJI Methylaspartate mutase, E subunit Spica_1665 ENOG4105C22 threonyL-tRNA synthetase Spica_1667 ENOG4108JIR Flagellum-specific ATP synthase Spica_1668 ENOG4105W2B Flagellar assembly protein Spica_1669 ENOG4105CS3 FliG is one of three proteins (FliG, FliN, FliM) that forms the rotor-mounted switch complex (C ring), located at the base of the basal body. This complex interacts with the CheY and CheZ chemotaxis proteins, in addition to contacting components of the motor that determine the direction of flagellar rotation (By similarity) Spica_1670 ENOG4107U2N Flagellar M-ring protein Spica_1671 ENOG41069WH flagellar hook-basal body complex Spica_1672 ENOG4108ZC9 flagellar basal-body rod protein flgc Spica_1673 ENOG4105Q75 Structural component of flagellum, the bacterial motility apparatus. Part of the rod structure of flagellar basal body (By similarity) Spica_1674 ENOG4105C4N this subunit has chaperone activity. The binding of ATP and its subsequent hydrolysis by HslU are essential for unfolding of protein substrates subsequently hydrolyzed by HslV. HslU recognizes the N-terminal part of its protein substrates and unfolds these before they are guided to HslV for hydrolysis (By similarity) Spica_1675 ENOG4108R5P Protease subunit of a proteasome-like degradation complex believed to be a general protein degrading machinery (By similarity) Spica_1676 ENOG4108I7H Site-specific tyrosine recombinase, which acts by catalyzing the cutting and rejoining of the recombining DNA molecules. The XerC-XerD complex is essential to convert dimers of the bacterial chromosome into monomers to permit their segregation at cell division. It also contributes to the segregational stability of plasmids (By similarity) Spica_1677 ENOG4105C73 Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity) Spica_1678 ENOG4105C79 DNA protecting protein DprA Spica_1681 ENOG4105CDK Essential cell division protein that forms a contractile ring structure (Z ring) at the future cell division site. The regulation of the ring assembly controls the timing and the location of cell division. One of the functions of the FtsZ ring is to recruit other cell division proteins to the septum to produce a new cell wall between the dividing cells. Binds GTP and shows GTPase activity (By similarity) Spica_1682 ENOG4105CIT This protein may be involved in anomalous filament growth. May be a component of the septum (By similarity) Spica_1684 ENOG4105CNI cell division protein FtsW Spica_1685 ENOG4105CPY First step of the lipid cycle reactions in the biosynthesis of the cell wall peptidoglycan (By similarity) Spica_1686 ENOG4107EES Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide the precursor of murein (By similarity) Spica_1688 ENOG4105CGJ Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA (By similarity) Spica_1689 ENOG4107YM1 mraZ protein Spica_1695 ENOG4105D2N Aldolase Spica_1697 ENOG4108UUX IF-3 binds to the 30S ribosomal subunit and shifts the equilibrum between 70S ribosomes and their 50S and 30S subunits in favor of the free subunits, thus enhancing the availability of 30S subunits on which protein synthesis initiation begins (By similarity) Spica_1698 ENOG41084MZ 50S ribosomal protein L35 Spica_1699 ENOG4108YZX Binds directly to 23S ribosomal RNA and is necessary for the in vitro assembly process of the 50S ribosomal subunit. It is not involved in the protein synthesizing functions of that subunit (By similarity) Spica_1701 ENOG4106BMT Activator of cell division through the inhibition of FtsZ GTPase activity, therefore promoting FtsZ assembly into bundles of protofilaments necessary for the formation of the division Z ring. It is recruited early at mid-cell but it is not essential for cell division (By similarity) Spica_1702 ENOG4105EIP mannose-6-phosphate isomerase Spica_1703 ENOG41082MQ UPF0178 protein Spica_1704 ENOG4105DW3 Response regulator receiver modulated metal dependent phosphohydrolase Spica_1705 ENOG4107UUG Plays a major role in protein secretion by helping the post-translocational extracellular folding of several secreted proteins (By similarity) Spica_1709 ENOG4105CPP Dehydrogenase, E1 component Spica_1710 ENOG4108JYK Dehydrogenase Spica_1712 ENOG4105BZY Part of the ABC transporter complex PstSACB involved in phosphate import. Responsible for energy coupling to the transport system (By similarity) Spica_1713 ENOG4105E2Z phosphate ABC transporter (Permease Spica_1714 ENOG4105C8U phosphate abc transporter Spica_1715 ENOG4107QMV Part of the ABC transporter complex PstSACB involved in phosphate import (By similarity) Spica_1722 ENOG4105D26 NQR complex catalyzes the reduction of ubiquinone-1 to ubiquinol by two successive reactions, coupled with the transport of Na( ) ions from the cytoplasm to the periplasm. The first step is catalyzed by NqrF, which accepts electrons from NADH and reduces ubiquinone-1 to ubisemiquinone by a one-electron transfer pathway (By similarity) Spica_1723 ENOG4108IUB NQR complex catalyzes the reduction of ubiquinone-1 to ubiquinol by two successive reactions, coupled with the transport of Na( ) ions from the cytoplasm to the periplasm. NqrA to NqrE are probably involved in the second step, the conversion of ubisemiquinone to ubiquinol (By similarity) Spica_1725 ENOG4108UQQ Electron transport complex Spica_1727 ENOG4107S5Z Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domain of GcvH, an intermediate carrier during protein lipoylation (By similarity) Spica_1729 ENOG4105C0G Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives (By similarity) Spica_1731 ENOG4106H3A restriction Spica_1753 ENOG4106H3A restriction Spica_1758 ENOG4106H3A restriction Spica_1782 ENOG4107TB6 phosphoglycerate mutase Spica_1795 ENOG4105D5G Transposase Spica_1796 ENOG4105C0H protease Spica_1803 ENOG4105C4F May be involved in recombinational repair of damaged DNA (By similarity) Spica_1804 ENOG4105F91 Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus (By similarity) Spica_1806 ENOG4105YFD chemotaxis, protein Spica_1809 ENOG4105C0H protease Spica_1810 ENOG4105CA1 Removes the N-terminal methionine from nascent proteins (By similarity) Spica_1811 ENOG4105BZ5 ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins (By similarity) Spica_1814 ENOG4106C6A Produces ATP from ADP in the presence of a proton gradient across the membrane (By similarity) Spica_1815 ENOG4108JIP Produces ATP from ADP in the presence of a proton gradient across the membrane. The V-type beta chain is a regulatory subunit (By similarity) Spica_1816 ENOG4107RVI Produces ATP from ADP in the presence of a proton gradient across the membrane. The V-type alpha chain is a catalytic subunit (By similarity) Spica_1820 ENOG4107WUB V-type ATPase 116 kDa subunit Spica_1823 ENOG4107RM2 PTS IIA-like nitrogen-regulatory protein PtsN Spica_1824 ENOG4106447 Preprotein translocase subunit SecG Spica_1825 ENOG4105CP7 Triose-phosphate isomerase Spica_1826 ENOG4105BZA Phosphoglycerate kinase Spica_1827 ENOG4105C17 glyceraldehyde-3-phosphate dehydrogenase Spica_1829 ENOG4105CA4 amino acids such as threonine, to avoid such errors, it has a posttransfer editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA-dependent manner (By similarity) Spica_1830 ENOG4105C48 Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) (By similarity) Spica_1831 ENOG4107XHD Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. SecDF uses the proton motive force (PMF) to complete protein translocation after the ATP-dependent function of SecA (By similarity) Spica_1832 ENOG4107QN8 Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. SecDF uses the proton motive force (PMF) to complete protein translocation after the ATP-dependent function of SecA (By similarity) Spica_1833 ENOG4105VIE preprotein translocase, subunit YajC Spica_1836 ENOG4105MAX Superoxide reductase Spica_1837 ENOG4108RAC Rubrerythrin Spica_1844 ENOG4105C2Z ATP-dependent CLP protease ATP-binding subunit Spica_1845 ENOG4105CGH 5'-nucleotidase Spica_1847 ENOG4105CM0 Catalyzes the synthesis of GMP from XMP (By similarity) Spica_1848 ENOG4105C91 Plays an important role in the de novo pathway of purine nucleotide biosynthesis Spica_1849 ENOG4105CP4 Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate- limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth (By similarity) Spica_1850 ENOG4107QTF adenylosuccinate lyase Spica_1852 ENOG410667J hydrolase Spica_1861 ENOG4105C2F Histidine kinase Spica_1862 ENOG4105EQD regulatoR Spica_1863 ENOG4105DFW ABC transporter Spica_1865 ENOG4105C2J ABC transporter Spica_1866 ENOG4108HWV )-transporter Spica_1873 ENOG4105DKZ GTPase that plays an essential role in the late steps of ribosome biogenesis (By similarity) Spica_1874 ENOG4108IJ0 Aspartate ammonia-lyase Spica_1875 ENOG4107QW8 radical SAM domain protein Spica_1876 ENOG4105D41 biosynthesis protein thiH Spica_1882 ENOG4105C8Q Methyl-accepting chemotaxis Spica_1884 ENOG4105DM4 PTS System Spica_1887 ENOG4105C6X Major Facilitator Spica_1889 ENOG4107RFY SbcCD D subunit Spica_1890 ENOG4105EYH Exonuclease Spica_1891 ENOG4105BZU Histidine kinase Spica_1892 ENOG4108RWI nicotinamidase Spica_1893 ENOG4105DAP Nicotinate phosphoribosyltransferase Spica_1894 ENOG4108QXE glycerophosphoryl diester phosphodiesterase Spica_1899 ENOG4106TNF Periplasmic binding protein Spica_1900 ENOG4105E5Q amino acid AbC transporter Spica_1901 ENOG4105CB3 Required for the thiolation of cytidine in position 32 of tRNA, to form 2-thiocytidine (s(2)C32) (By similarity) Spica_1907 ENOG4105F66 Binding-protein-dependent transport systems inner membrane component Spica_1908 ENOG4105DB9 Binding-protein-dependent transport systems inner membrane component Spica_1909 ENOG4107T3P ABC, transporter Spica_1910 ENOG4107414 GntR family transcriptional regulator Spica_1917 ENOG4105NDF NAD-dependent protein deacetylase which modulates the activities of several enzymes which are inactive in their acetylated form. May also have NAD-dependent lysine demalonylase and desuccinylase activity (By similarity) Spica_1923 ENOG4105KCF CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Functions as a ssRNA-specific endoribonuclease (By similarity) Spica_1924 ENOG4105DYN CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Acts as a dsDNA endonuclease. May be involved in the integration of spacer DNA into the CRISPR cassette (By similarity) Spica_1928 ENOG4105FB9 CRISPR-associated protein Spica_1931 ENOG4105DA0 Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) (By similarity) Spica_1933 ENOG4105E2D ec 3.2.1.52 Spica_1936 ENOG4105E0X reductase Spica_1937 ENOG4105CCW damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed. This complex is subsequently bound by UvrC and the second UvrB is released. If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage (By similarity) Spica_1939 ENOG4105Z8J Spica_1940 ENOG4108H5C Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate (By similarity) Spica_1941 ENOG41084P6 50S ribosomal protein l28 Spica_1945 ENOG4105C8Q Methyl-accepting chemotaxis Spica_1947 ENOG4105C6P ATP-dependent serine protease that mediates the selective degradation of mutant and abnormal proteins as well as certain short-lived regulatory proteins. Required for cellular homeostasis and for survival from DNA damage and developmental changes induced by stress. Degrades polypeptides processively to yield small peptide fragments that are 5 to 10 amino acids long. Binds to DNA in a double-stranded, site-specific manner (By similarity) Spica_1954 ENOG4105C9U ATP-dependent DNA helicase RecQ Spica_1957 ENOG4107QYT Catalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor (By similarity) Spica_1958 ENOG4105CS3 FliG is one of three proteins (FliG, FliN, FliM) that forms the rotor-mounted switch complex (C ring), located at the base of the basal body. This complex interacts with the CheY and CheZ chemotaxis proteins, in addition to contacting components of the motor that determine the direction of flagellar rotation (By similarity) Spica_1961 ENOG4105CB5 ATP-dependent DNA helicase recG Spica_1963 ENOG4105BZU Histidine kinase Spica_1964 ENOG4105C0B DNA polymerase III (alpha subunit) Spica_1965 ENOG4105ETE Transcriptional regulator (LacI family Spica_1966 ENOG4105CHM Aminotransferase Spica_1968 ENOG41084YP 30S ribosomal protein S21 Spica_1969 ENOG4105DR5 peptidase Spica_1970 ENOG4105C2R Single-stranded-DNA-specific exonuclease (RecJ) Spica_1973 ENOG4108DQ3 n-acetylglutamate synthase Spica_1974 ENOG4107RTZ reductase Spica_1979 ENOG4105CC8 Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism (By similarity) Spica_1980 ENOG4105CEH Catalyzes the synthesis of dihydrouridine a modified base found in the D-loop of most tRNAs (By similarity) Spica_1981 ENOG4105N2W Transcriptional regulator Spica_1982 ENOG4107QKR Dehydrogenase Spica_1983 ENOG4107QN2 dihydrolipoyl dehydrogenase Spica_1984 ENOG4105CPP Dehydrogenase, E1 component Spica_1986 ENOG4105CHR reductase Spica_1988 ENOG4105DXH Alcohol dehydrogenase zinc-binding domain protein Spica_1993 ENOG4105E4P ATPase (AAA Spica_1994 ENOG4105C8Q Methyl-accepting chemotaxis Spica_2000 ENOG4107RBB ABC transporter, permease Spica_2001 ENOG4105D6C Part of the ABC transporter complex MacAB involved in macrolide export. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation (By similarity) Spica_2002 ENOG4105D6C Part of the ABC transporter complex MacAB involved in macrolide export. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation (By similarity) Spica_2004 ENOG4105BZU Histidine kinase Spica_2005 ENOG4105DPZ Two component transcriptional regulator (Winged helix family Spica_2007 ENOG4108HWW ABC transporter Spica_2009 ENOG4105DFW ABC transporter Spica_2010 ENOG4105DFW ABC transporter Spica_2011 ENOG4108VB1 regulatoR Spica_2012 ENOG4105C2F Histidine kinase Spica_2014 ENOG4108R4Z Functions in the N-end rule pathway of protein degradation where it conjugates Leu, Phe and, less efficiently, Met from aminoacyl-tRNAs to the N-termini of proteins containing an N-terminal arginine or lysine (By similarity) Spica_2015 ENOG4105C2Z ATP-dependent CLP protease ATP-binding subunit Spica_2016 ENOG4105KAE Involved in the modulation of the specificity of the ClpAP-mediated ATP-dependent protein degradation (By similarity) Spica_2019 ENOG4105CGK Exodeoxyribonuclease III Spica_2024 ENOG4105DJY efflux transporter, rnd family, mfp subunit Spica_2026 ENOG4105CAV thus facilitating recognition of the initiation point. It is needed to translate mRNA with a short Shine-Dalgarno (SD) purine-rich sequence (By similarity) Spica_2029 ENOG4105DSF transport protein Spica_2031 ENOG4105CJM Binding-protein-dependent transport systems, inner membrane component Spica_2032 ENOG4105C2T Binding-protein-dependent transport systems, inner membrane component Spica_2034 ENOG4108JQ7 (ABC) transporter Spica_2035 ENOG4105CCZ Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Catalyzes the first condensation reaction which initiates fatty acid synthesis and may therefore play a role in governing the total rate of fatty acid production. Possesses both acetoacetyl-ACP synthase and acetyl transacylase activities. Its substrate specificity determines the biosynthesis of branched- chain and or straight-chain of fatty acids (By similarity) Spica_2037 ENOG4105CHR reductase Spica_2038 ENOG4105C0Q Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP (By similarity) Spica_2039 ENOG4108YXN Involved in unsaturated fatty acids biosynthesis. Catalyzes the dehydration of short chain beta-hydroxyacyl-ACPs and long chain saturated and unsaturated beta-hydroxyacyl-ACPs (By similarity) Spica_2040 ENOG4105RKW reductase (By Spica_2041 ENOG4105KM4 Acetyl-CoA carboxylase, biotin carboxyl carrier protein Spica_2042 ENOG4105CER acetyl-CoA carboxylase biotin carboxylase Spica_2043 ENOG4107QTG Component of the acetyl coenzyme A carboxylase (ACC) complex. Biotin carboxylase (BC) catalyzes the carboxylation of biotin on its carrier protein (BCCP) and then the CO(2) group is transferred by the transcarboxylase to acetyl-CoA to form malonyl- CoA (By similarity) Spica_2044 ENOG4107QM9 Component of the acetyl coenzyme A carboxylase (ACC) complex. First, biotin carboxylase catalyzes the carboxylation of biotin on its carrier protein (BCCP) and then the CO(2) group is transferred by the carboxyltransferase to acetyl-CoA to form malonyl-CoA (By similarity) Spica_2046 ENOG41076HS cation diffusion facilitator family transporter Spica_2048 ENOG4105C46 Catalyzes the first step in hexosamine metabolism, converting fructose-6P into glucosamine-6P using glutamine as a nitrogen source (By similarity) Spica_2050 ENOG4108SP1 single-stranded DNA-binding protein Spica_2051 ENOG4105CFP Peptidase U32 Spica_2052 ENOG4105EZI multiple antibiotic resistance (MarC)-related protein Spica_2056 ENOG4107RMF Catalyzes both the ATP-dependent activation of exogenously supplied lipoate to lipoyl-AMP and the transfer of the activated lipoyl onto the lipoyl domains of lipoate-dependent enzymes (By similarity) Spica_2064 ENOG4105BZU Histidine kinase Spica_2066 ENOG4105CTQ phosphohexokinase Spica_2068 ENOG4105CJQ hydrolase, family 31 Spica_2073 ENOG4105EQ7 Binding-protein-dependent transport systems, inner membrane component Spica_2077 ENOG4105CUZ uba thif-type nad fad binding protein Spica_2080 ENOG4105C3X it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP (By similarity) Spica_2084 ENOG4105BZK Transcription regulator that activates transcription by stimulating RNA polymerase (RNAP) recycling in case of stress conditions such as supercoiled DNA or high salt concentrations. Probably acts by releasing the RNAP, when it is trapped or immobilized on tightly supercoiled DNA. Does not activate transcription on linear DNA. Probably not involved in DNA repair (By similarity) Spica_2087 ENOG4108HHA Transketolase Spica_2088 ENOG4108IUS Transketolase Spica_2089 ENOG4108YZG isomerase Spica_2090 ENOG4105C0A alcohol dehydrogenase Spica_2094 ENOG4105D1E Undecaprenyl pyrophosphate phosphatase Spica_2095 ENOG4105DW3 Response regulator receiver modulated metal dependent phosphohydrolase Spica_2098 ENOG4105C1H Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 (By similarity) Spica_2099 ENOG4107RE5 NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34 (By similarity) Spica_2107 ENOG4108UH8 Involved in the biosynthesis of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP), two major building blocks of isoprenoid compounds. Catalyzes the conversion of 4- diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate (CDP-ME2P) to 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-CPP) with a corresponding release of cytidine 5-monophosphate (CMP) (By similarity) Spica_2108 ENOG4108UH8 Involved in the biosynthesis of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP), two major building blocks of isoprenoid compounds. Catalyzes the conversion of 4- diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate (CDP-ME2P) to 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-CPP) with a corresponding release of cytidine 5-monophosphate (CMP) (By similarity) Spica_2109 ENOG4105CSM endonuclease III Spica_2113 ENOG4109015 Biopolymer transport protein exbD tolR Spica_2119 ENOG4107QW3 Sucrose phosphorylase Spica_2127 ENOG4105EPM Diguanylate phosphodiesterase Spica_2128 ENOG4105C8Q Methyl-accepting chemotaxis Spica_2129 ENOG4105E06 May play a role in 30S ribosomal subunit biogenesis. Unusual circulary permuted GTPase that catalyzes rapid hydrolysis of GTP with a slow catalytic turnover (By similarity) Spica_2130 ENOG4105WH1 Two component transcriptional regulator luxr family Spica_2131 ENOG4105ENE Histidine kinase Spica_2133 ENOG4108IUW ABC transporter Spica_2139 ENOG4105DW3 Response regulator receiver modulated metal dependent phosphohydrolase Spica_2140 ENOG4105C53 Part of the ABC transporter complex PotABCD involved in spermidine putrescine import. Responsible for energy coupling to the transport system (By similarity) Spica_2141 ENOG4105F38 ABC transporter, permease Spica_2142 ENOG4105D38 putrescine abc transporter Spica_2143 ENOG4107RUE extracellular solute-binding protein family 1 Spica_2144 ENOG4105EK0 Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine (By similarity) Spica_2146 ENOG4105C1W two component, sigma54 specific, transcriptional regulator, Fis family Spica_2147 ENOG4105C2F Histidine kinase Spica_2150 ENOG4105CNN ABC, transporter Spica_2152 ENOG4105NXV High-affinity zinc uptake system protein Spica_2153 ENOG4105FDK ABC transporter Spica_2157 ENOG4108Q5Y Membrane Spica_2163 ENOG4105DUM transcriptional regulator, lysR family Spica_2164 ENOG4105HMI Membrane Spica_2167 ENOG4105C56 H( )-stimulated, divalent metal cation uptake system (By similarity) Spica_2169 ENOG4105C52 Atpase, p-type (Transporting), had superfamily, subfamily ic Spica_2171 ENOG4105KME endoribonuclease L-psp Spica_2180 ENOG4105CV2 Nucleotidase that shows phosphatase activity on nucleoside 5'-monophosphates (By similarity) Spica_2213 ENOG4105C2F Histidine kinase Spica_2214 ENOG4105EQD regulatoR Spica_2215 ENOG4107R5U Periplasmic binding protein LacI transcriptional regulator Spica_2216 ENOG4107R5U Periplasmic binding protein LacI transcriptional regulator Spica_2217 ENOG4105C2J ABC transporter Spica_2218 ENOG4107R9N ABC transporter Spica_2219 ENOG41062B0 ABC transporter, permease Spica_2222 ENOG4105EX2 Binding-protein-dependent transport systems, inner membrane component Spica_2224 ENOG4105ETE Transcriptional regulator (LacI family Spica_2239 ENOG4105D6C Part of the ABC transporter complex MacAB involved in macrolide export. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation (By similarity) Spica_2241 ENOG4105W8V TRANSCRIPTIONAl REGULATOR GntR family Spica_2245 ENOG4105NWF RbsD or FucU transport Spica_2247 ENOG4105DXH Alcohol dehydrogenase zinc-binding domain protein Spica_2248 ENOG4105DE4 1-phosphofructokinase Spica_2249 ENOG4105KRI phosphocarrier protein (HPr Spica_2250 ENOG4105BZ3 General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. Enzyme I transfers the phosphoryl group from phosphoenolpyruvate (PEP) to the phosphoryl carrier protein (HPr) (By similarity) Spica_2252 ENOG4105ETD TRANSCRIPTIONal Spica_2255 ENOG4105DCG PHP domain protein Spica_2256 ENOG4105UQX Mate efflux family protein Spica_2258 ENOG4107B2H carbonic anhydrase Spica_2261 ENOG4108AQF aminoglycoside N(3)-acetyltransferase Spica_2270 ENOG4105CHH sulfate transporter Spica_2273 ENOG4105BZ1 (ABC) transporter Spica_2283 ENOG4105ENE Histidine kinase Spica_2284 ENOG4105WH1 Two component transcriptional regulator luxr family Spica_2285 ENOG4105XN3 extracellular solute-binding protein family 1 Spica_2286 ENOG4105F0P binding-protein-dependent transport systems inner membrane Component Spica_2289 ENOG4105MK2 5-formyltetrahydrofolate cyclo-ligase Spica_2293 ENOG4108V1J Has antioxidant activity. Could remove peroxides or H(2)O(2) (By similarity) Spica_2298 ENOG4105EBR Carboxylesterase Spica_2301 ENOG4107Y3V -acetyltransferase Spica_2306 ENOG4105DH2 Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group (By similarity) Spica_2308 ENOG4105D10 5-enolpyruvylshikimate-3-phosphate phospholyase Spica_2309 ENOG4105C7R Atpase associated with various cellular activities aaa_3 Spica_2315 ENOG4105C7R Atpase associated with various cellular activities aaa_3 Spica_2316 ENOG41062B0 ABC transporter, permease Spica_2317 ENOG4107R9N ABC transporter Spica_2318 ENOG4105C2J ABC transporter Spica_2319 ENOG4107R5U Periplasmic binding protein LacI transcriptional regulator Spica_2320 ENOG4107R5U Periplasmic binding protein LacI transcriptional regulator Spica_2321 ENOG4105C2F Histidine kinase Spica_2322 ENOG4105EQD regulatoR Spica_2323 ENOG4108IQF Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA (By similarity) Spica_2326 ENOG4105E66 phosphoriboisomerase A Spica_2328 ENOG4105E2X shikimate dehydrogenase Spica_2330 ENOG4108IQE Amp-dependent synthetase and ligase Spica_2332 ENOG4108120 Pasta domain protein Spica_2333 ENOG4105CAE Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by (I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-GTP (By similarity) Spica_2334 ENOG4108Z02 Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions (By similarity) Spica_2335 ENOG4108JQB penicillin-binding protein 1C Spica_2336 ENOG4105DN3 alpha-2-macroglobulin domain protein Spica_2338 ENOG4105CMP catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR (By similarity) Spica_2339 ENOG4108Z4W Probably deamidates glutamine residues to glutamate on methyl-accepting chemotaxis receptors (MCPs), playing an important role in chemotaxis (By similarity) Spica_2340 ENOG4105BZU Histidine kinase Spica_2341 ENOG4105C8Q Methyl-accepting chemotaxis Spica_2342 ENOG4108V3W Chemotaxis protein, CheW Spica_2344 ENOG4105CBS Chea signal transduction histidine kinase Spica_2347 ENOG4105CPQ Dehydrogenase Spica_2351 ENOG4105D86 that it carries out the mismatch recognition step. This protein has a weak ATPase activity (By similarity) Spica_2352 ENOG4105C98 muts2 protein Spica_2357 ENOG4105D43 The glycine cleavage system catalyzes the degradation of glycine (By similarity) Spica_2358 ENOG4105KE9 The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein (By similarity) Spica_2360 ENOG4107RGW The glycine cleavage system catalyzes the degradation of glycine. The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor Spica_2363 ENOG4105K85 Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction the enzyme is irreversibly inactivated (By similarity) Spica_2365 ENOG4108D32 Methyltransferase Spica_2367 ENOG4105C6H Catalyzes the formation of acetyl phosphate from acetate and ATP. Can also catalyze the reverse reaction (By similarity) Spica_2369 ENOG4107S0N DJ-1 family Spica_2370 ENOG4105CK6 Two component transcriptional regulator (Winged helix family Spica_2371 ENOG4105BZU Histidine kinase Spica_2372 ENOG4108W76 histidinol phosphate phosphatase hisj family Spica_2374 ENOG4105K9U however, it seems to stimulate more or less all the activities of the other two initiation factors, IF-2 and IF-3 (By similarity) Spica_2375 ENOG4108DG9 Smr protein MutS2 Spica_2382 ENOG4105C7R Atpase associated with various cellular activities aaa_3 Spica_2387 ENOG4105C0M Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle (By similarity) Spica_2390 ENOG4105C3G gtp-binding protein Spica_2392 ENOG4105CM2 brancheD-chain amino acid aminotransferase Spica_2394 ENOG4105C22 threonyL-tRNA synthetase Spica_2398 ENOG4107QIK phosphoribosylformylglycinamidine synthase Spica_2399 ENOG4105C8Q Methyl-accepting chemotaxis Spica_2406 ENOG4105D28 Specifically methylates the N7 position of a guanine in 16S rRNA (By similarity) Spica_2408 ENOG4105C8Q Methyl-accepting chemotaxis Spica_2410 ENOG4107FQX Alpha-acetolactate decarboxylase Spica_2412 ENOG4105C8Q Methyl-accepting chemotaxis Spica_2413 ENOG4105C8V SAICAR synthetase Spica_2416 ENOG4108V3E phosphoribosylglycinamide formyltransferase Spica_2417 ENOG4105DC1 bifunctional purine biosynthesis protein purh Spica_2418 ENOG4105C12 Phosphoribosylglycinamide synthetase Spica_2419 ENOG4105EC0 Required for nucleoid occlusion (NO) phenomenon, which prevents Z-ring formation and cell division over the nucleoid. Acts as a DNA-associated cell division inhibitor that binds simultaneously chromosomal DNA and FtsZ, and disrupts the assembly of FtsZ polymers. SlmA-DNA-binding sequences (SBS) are dispersed on non-Ter regions of the chromosome, preventing FtsZ polymerization at these regions (By similarity) Spica_2420 ENOG4105C50 Binding-protein-dependent transport systems, inner membrane component Spica_2423 ENOG4105BZ1 (ABC) transporter Spica_2424 ENOG4105BZ1 (ABC) transporter Spica_2427 ENOG4105CP9 extracellular ligand-binding receptor Spica_2428 ENOG4105CEY Amp-dependent synthetase and ligase Spica_2432 ENOG4105E21 Catalyzes the condensation of ATP and 5-phosphoribose 1- diphosphate to form N'-(5'-phosphoribosyl)-ATP (PR-ATP). Has a crucial role in the pathway because the rate of histidine biosynthesis seems to be controlled primarily by regulation of HisG enzymatic activity (By similarity) Spica_2433 ENOG4105ECC imidazole-glycerol-phosphate dehydratase Spica_2434 ENOG4108UJE IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to HisF for the synthesis of IGP and AICAR (By similarity) Spica_2436 ENOG4105K8F Phosphoribosyl-amp cyclohydrolase Spica_2437 ENOG4105CIH Imidazole acetol-phosphate transaminase Spica_2438 ENOG4105CEK Catalyzes the sequential NAD-dependent oxidations of L- histidinol to L-histidinaldehyde and then to L-histidine (By similarity) Spica_2444 ENOG4107QWR cellulase (EC 3.2.1.4) Spica_2450 ENOG4108W3Q Transcriptional regulator Spica_2452 ENOG4105C50 Binding-protein-dependent transport systems, inner membrane component Spica_2454 ENOG4105E0S extracellular solute-binding protein family 1 Spica_2455 ENOG4105E4I Glycoside hydrolase family 28 Spica_2463 ENOG4105CY8 phosphoribosylaminoimidazole carboxylase atpase subunit Spica_2464 ENOG4108UM6 Catalyzes the conversion of N5-carboxyaminoimidazole ribonucleotide (N5-CAIR) to 4-carboxy-5-aminoimidazole ribonucleotide (CAIR) (By similarity) Spica_2472 ENOG4105CE4 GlcNAc 6-P deacetylase Spica_2473 ENOG4105CKA Catalyzes the reversible isomerization-deamination of glucosamine 6-phosphate (GlcN6P) to form fructose 6-phosphate (Fru6P) and ammonium ion (By similarity) Spica_2475 ENOG4105CJW magnesium transporter Spica_2476 ENOG4107N47 CrcB-like protein Spica_2477 ENOG4105KJJ Uncharacterized ACR, COG1993 Spica_2482 ENOG4105EH2 Catalyzes the conversion of epoxyqueuosine (oQ) to queuosine (Q), which is a hypermodified base found in the wobble positions of tRNA(Asp), tRNA(Asn), tRNA(His) and tRNA(Tyr) (By similarity) Spica_2484 ENOG4105D95 Oxidoreductase required for the transfer of electrons from pyruvate to flavodoxin (By similarity) Spica_2485 ENOG4105C1W two component, sigma54 specific, transcriptional regulator, Fis family Spica_2486 ENOG4105IRK Glycosyl transferase (Group 1 Spica_2487 ENOG4105C6Z Gluconate Spica_2488 ENOG4105DXN mazG family Spica_2489 ENOG4108ZPD The natural substrate for this enzyme may be peptidyl- tRNAs which drop off the ribosome during protein synthesis (By similarity) Spica_2490 ENOG4107R49 K07407 alpha-galactosidase EC 3.2.1.22 Spica_2491 ENOG4105E2M Manganese-dependent inorganic pyrophosphatase Spica_2494 ENOG4108S10 Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins (By similarity) Spica_2496 ENOG41082J7 Rhodanese domain protein Spica_2498 ENOG4105CKU formyltetrahydrofolate synthetase Spica_2499 ENOG4105CJ4 Catalyzes the interconversion of L-alanine and D- alanine. May also act on other amino acids (By similarity) Spica_2505 ENOG4105EAD ABC transporter Spica_2506 ENOG4108HW3 (ABC) transporter Spica_2509 ENOG4108RCM Nitroreductase Spica_2511 ENOG4105CJR Integral membrane protein (MviN Spica_2514 ENOG4105CMY 5-enolpyruvylshikimate-3-phosphate synthase Spica_2516 ENOG4105D37 Low-affinity potassium transport system. Interacts with Trk system potassium uptake protein TrkA (By similarity) Spica_2517 ENOG4105DK7 lysyl-tRNA synthetase Spica_2518 ENOG4105C59 p-type ATPase Spica_2519 ENOG410681U heavy metal transport detoxification protein Spica_2521 ENOG4105BZU Histidine kinase Spica_2528 ENOG4105C26 Dehydrogenase Spica_2529 ENOG4105C7N Xanthine uracil vitamin C permease Spica_2535 ENOG4108JQ7 (ABC) transporter Spica_2537 ENOG4105C2T Binding-protein-dependent transport systems, inner membrane component Spica_2538 ENOG4105CJM Binding-protein-dependent transport systems, inner membrane component Spica_2539 ENOG4108J59 Extracellular solute-binding protein, family 5 Spica_2542 ENOG4105T73 Spica_2545 ENOG4105C8Q Methyl-accepting chemotaxis Spica_2552 ENOG4105SKN orotidine 5''-phosphate decarboxylase Spica_2553 ENOG4105DF0 Responsible for channeling the electrons from the oxidation of dihydroorotate from the FMN redox center in the PyrD type B subunit to the ultimate electron acceptor NAD( ) (By similarity) Spica_2554 ENOG4107W93 Dihydroorotate dehydrogenase Spica_2560 ENOG4105DKZ GTPase that plays an essential role in the late steps of ribosome biogenesis (By similarity) Spica_2561 ENOG4105BZ3 General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. Enzyme I transfers the phosphoryl group from phosphoenolpyruvate (PEP) to the phosphoryl carrier protein (HPr) (By similarity) Spica_2566 ENOG4105DNJ May play a role in the repair of endogenous alkylation damage (By similarity) Spica_2567 ENOG4105VFU Rna-binding protein Spica_2574 ENOG4108JQ1 Dehydrogenase Spica_2575 ENOG4107QM1 Catalyzes the reversible conversion of 3- phosphohydroxypyruvate to phosphoserine and of 3-hydroxy-2-oxo-4- phosphonooxybutanoate to phosphohydroxythreonine (By similarity) Spica_2576 ENOG4105FF6 Modulates transcription in response to changes in cellular NADH NAD( ) redox state (By similarity) Spica_2577 ENOG4105D1C Catalyzes the ATP- as well as the pyrophosphate- dependent phosphorylation of a specific serine residue in HPr, a phosphocarrier protein of the phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS). HprK P also catalyzes the pyrophosphate-producing, inorganic phosphate-dependent dephosphorylation (phosphorolysis) of seryl-phosphorylated HPr (P- Ser-HPr). The two antagonistic activities of HprK P are regulated by several intracellular metabolites, which change their concentration in response to the absence or presence of rapidly metabolisable carbon sources (glucose, fructose, etc.) in the growth medium. Therefore, by controlling the phosphorylation state of HPr, HPrK P is a sensor enzyme that plays a major role in the regulation of carbon metabolism and sugar transport it mediates carbon catabolite repression (CCR), and regulates PTS-catalyzed carbohydrate uptake and inducer exclusion (By similarity) Spica_2578 ENOG4105KZ7 phosphocarrier protein hpr Spica_2579 ENOG4105ES5 Histidine kinase Spica_2580 ENOG4105C1W two component, sigma54 specific, transcriptional regulator, Fis family Spica_2581 ENOG4105DS7 Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, RecA interacts with LexA causing an autocatalytic cleavage which disrupts the DNA-binding part of LexA, leading to derepression of the SOS regulon and eventually DNA repair (By similarity) Spica_2583 ENOG4105CEP NADH dehydrogenase Spica_2586 ENOG4105DR4 Methyltransferase Spica_2587 ENOG4105CUU Catalyzes the reversible transfer of the terminal phosphate of ATP to form a long-chain polyphosphate (polyP) (By similarity) Spica_2588 ENOG4105C9X ppx gppa phosphatase Spica_2589 ENOG4107MD5 glycoside hydrolase, family Spica_2590 ENOG4105CHT Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln) (By similarity) Spica_2591 ENOG410902C phosphohistidine phosphatase, SixA Spica_2598 ENOG4105D05 Receptor Spica_2600 ENOG4105EAD ABC transporter Spica_2601 ENOG4106PMH Extracellular solute-binding protein family 3 Spica_2603 ENOG4105DPZ Two component transcriptional regulator (Winged helix family Spica_2604 ENOG4105EB3 Histidine kinase Spica_2605 ENOG4105CJ1 ABC, transporter Spica_2610 ENOG4105VFU Rna-binding protein Spica_2611 ENOG4105C8Q Methyl-accepting chemotaxis Spica_2612 ENOG4105DW3 Response regulator receiver modulated metal dependent phosphohydrolase Spica_2614 ENOG4107S1W 4-alpha-glucanotransferase Spica_2617 ENOG4105D98 threonine synthase Spica_2618 ENOG4106PMH Extracellular solute-binding protein family 3 Spica_2619 ENOG4105E5Q amino acid AbC transporter Spica_2621 ENOG4108UXE Protein tyrosine phosphatase Spica_2622 ENOG41082YX Transcriptional regulator, arsr family Spica_2625 ENOG4105C7Q Catalyzes the oxidative decarboxylation of 6- phosphogluconate to ribulose 5-phosphate and CO(2), with concomitant reduction of NADP to NADPH (By similarity) Spica_2626 ENOG4105ETE Transcriptional regulator (LacI family Spica_2627 ENOG4105CM0 Catalyzes the synthesis of GMP from XMP (By similarity) Spica_2628 ENOG4105CG6 trap dicarboxylate transporter dctm subunit Spica_2630 ENOG4105DR6 Trap dicarboxylate transporter, dctp subunit Spica_2631 ENOG4105EQD regulatoR Spica_2632 ENOG4108HRK Histidine kinase Spica_2633 ENOG4105BZU Histidine kinase Spica_2634 ENOG4105K4H Nlp p60 protein Spica_2639 ENOG4107RE7 phosphate butyryltransferase Spica_2640 ENOG4105CKM Branched-chain carboxylic acid kinase Spica_2643 ENOG4105D42 Cleaves the N-terminal amino acid of tripeptides (By similarity) Spica_2648 ENOG4105CZV ABC transporter Spica_2650 ENOG4105DZ0 fad dependent oxidoreductase Spica_2651 ENOG4105HCZ Spica_2652 ENOG4105HKU nucleoside recognition domain-containing protein Spica_2654 ENOG4105C5H ABC transporter Spica_2655 ENOG4105BZU Histidine kinase Spica_2657 ENOG4105UIB (ABC) transporter Spica_2658 ENOG4105C2J ABC transporter Spica_2659 ENOG4108SSC basic membrane Spica_2664 ENOG4105MZG transcriptional modulator of maze toxin, mazf Spica_2668 ENOG4105EMS pseudouridine synthase Spica_2670 ENOG4105C2Y STE24 endopeptidase EC 3.4.24.84 Spica_2671 ENOG4105C7K acetolactate synthase Spica_2672 ENOG4105C01 Dihydroxy-acid dehydratase Spica_2673 ENOG4105C0C Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate (By similarity) Spica_2674 ENOG4105CYQ Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) (By similarity) Spica_2675 ENOG4105CYQ Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) (By similarity) Spica_2676 ENOG4105C6M Alpha-keto-beta-hydroxylacyl reductoisomerase Spica_2677 ENOG4108ZP8 Acetolactate synthase small subunit Spica_2678 ENOG4108JPM phospho-2-dehydro-3-deoxyheptonate aldolase Spica_2683 ENOG4105D7X high-affinity nickel-transporter Spica_2686 ENOG4105D1W Converts 2,5-diamino-6-(ribosylamino)-4(3h)-pyrimidinone 5'-phosphate into 5-amino-6-(ribosylamino)-2,4(1h,3h)- pyrimidinedione 5'-phosphate (By similarity) Spica_2687 ENOG4105C01 Dihydroxy-acid dehydratase Spica_2688 ENOG4105CQI Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate (By similarity) Spica_2690 ENOG4105C7K acetolactate synthase Spica_2691 ENOG4108ZP8 Acetolactate synthase small subunit Spica_2692 ENOG41079KZ Major Facilitator Superfamily Spica_2695 ENOG4105DZR Type I site-specific deoxyribonuclease Spica_2697 ENOG4105EKX Restriction modification system DNA (Specificity Spica_2702 ENOG4105CVR type I restriction-modification system Spica_2704 ENOG41071TK Uncharacterised protein family (UPF0150) Spica_2705 ENOG4105BZU Histidine kinase Spica_2706 ENOG4105EK0 Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine (By similarity) Spica_2708 ENOG4105D7N magnesium and cobalt transport protein CorA Spica_2709 ENOG4105E08 Glycoside hydrolase, family 9 Spica_2712 ENOG4106YVY Bifunctional enzyme that catalyzes the enolization of 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) into the intermediate 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HK-MTPenyl-1-P), which is then dephosphorylated to form the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene) (By similarity) Spica_2714 ENOG4105C8T Leucyl-tRNA synthetase Spica_2715 ENOG4105W06 Cupin 2, conserved barrel domain protein Spica_2717 ENOG4105E2K Glycosyl transferase (Group 1 Spica_2719 ENOG4105DC7 peptidase M24 Spica_2723 ENOG4105C6V Glycerol-3-phosphate dehydrogenase Spica_2726 ENOG4108HU6 Metal dependent phosphohydrolase Spica_2729 ENOG4105CII The UvrABC repair system catalyzes the recognition and processing of DNA lesions. UvrC both incises the 5' and 3' sides of the lesion. The N-terminal half is responsible for the 3' incision and the C-terminal half is responsible for the 5' incision (By similarity) Spica_2731 ENOG4105DTW Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released (By similarity) Spica_2733 ENOG4108VXD carboxy-peptidase Spica_2737 ENOG4107S03 uridine kinase Spica_2738 ENOG4105C8J )-transporter Spica_2739 ENOG4105BZ5 ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins (By similarity) Spica_2740 ENOG4105CFG Acts as a chaperone (By similarity) Spica_2741 ENOG4105K90 Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with DnaK and GrpE. It is the nucleotide exchange factor for DnaK and may function as a thermosensor. Unfolded proteins bind initially to DnaJ Spica_2743 ENOG4105DWR Catalyzes the dephosphorylation of undecaprenyl diphosphate (UPP). Confers resistance to bacitracin (By similarity) Spica_2745 ENOG4105C93 Xylose Isomerase Spica_2746 ENOG4105CMG Carbohydrate kinase Spica_2748 ENOG4105DPZ Two component transcriptional regulator (Winged helix family Spica_2749 ENOG4105RAK Histidine kinase Spica_2751 ENOG4105DHJ ABC, transporter Spica_2755 ENOG4105CAK Cell division ATP-binding protein ftsE Spica_2758 ENOG4105D48 metallophosphoesterase Spica_2759 ENOG4105E4Y Endoribonuclease that initiates mRNA decay (By similarity) Spica_2760 ENOG4108IUZ ABC transporter Spica_2763 ENOG4105C8D Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen (By similarity) Spica_2765 ENOG4105BZU Histidine kinase Spica_2766 ENOG4105CMP catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR (By similarity) Spica_2768 ENOG4108VKV Monofunctional biosynthetic peptidoglycan transglycosylase Spica_2770 ENOG4105D48 metallophosphoesterase Spica_2773 ENOG4107QHI -hydrogenase Spica_2774 ENOG4107QIZ NADH dehydrogenase Spica_2777 ENOG410902H NADH dehydrogenase (Ubiquinone), 24 kDa subunit Spica_2779 ENOG4105CEY Amp-dependent synthetase and ligase Spica_2783 ENOG4105CP4 Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate- limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth (By similarity) Spica_2784 ENOG410667J hydrolase Spica_2787 ENOG4107QJE Chromosome Partitioning Protein Spica_2788 ENOG4105FA0 parb-like partition protein Spica_2790 ENOG4105C2N RNA methyltransferase TrmH family group 3 Spica_2792 ENOG4108JEB The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine (By similarity) Spica_2793 ENOG4107R8N Glycosyl transferase, family 2 Spica_2800 ENOG4108JJB ABC transporter Spica_2801 ENOG4105C7J ABC transporter, permease Spica_2803 ENOG4105C6K Malic enzyme Spica_2806 ENOG4105DT3 PTS system, galactitol-specific IIc component Spica_2807 ENOG4105C90 Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys- tRNA(Pro) is not edited by ProRS (By similarity) Spica_2811 ENOG4105E27 serine threonine protein kinase Spica_2812 ENOG4105C0N N-acetylglutamate semialdehyde dehydrogenase Spica_2813 ENOG4108HWW ABC transporter Spica_2814 ENOG4105C2J ABC transporter Spica_2815 ENOG4105DFW ABC transporter Spica_2816 ENOG4105E98 transcriptional regulator DeoR family Spica_2817 ENOG4105C2J ABC transporter Spica_2819 ENOG4105CAC Catalyzes the conversion of L-arabinose to L-ribulose (By similarity) Spica_2821 ENOG4107QJQ Mate efflux family protein Spica_2826 ENOG4105C2N RNA methyltransferase TrmH family group 3 Spica_2827 ENOG4105CY0 Metallo-Beta-Lactamase Spica_2828 ENOG4105CF3 3-Hydroxyisobutyrate dehydrogenase Spica_2831 ENOG4105JPG D-isomer specific 2-hydroxyacid dehydrogenase Spica_2832 ENOG4105C3R Methionine synthase Spica_2836 ENOG4108V2V 5 nucleotidase deoxy cytosolic type C Spica_2837 ENOG4108IPX Part of the ABC transporter complex HmuTUV involved in hemin import. Responsible for energy coupling to the transport system (By similarity) Spica_2843 ENOG4107ZYR domain protein Spica_2844 ENOG4105CZN Potassium uptake protein Spica_2847 ENOG4105DB9 Binding-protein-dependent transport systems inner membrane component Spica_2850 ENOG4105CPQ Dehydrogenase Spica_2852 ENOG4105C7R Atpase associated with various cellular activities aaa_3 Spica_2857 ENOG4108IQE Amp-dependent synthetase and ligase Spica_2858 ENOG4105CPH Catalyzes the formation of S-adenosylmethionine from methionine and ATP Spica_2859 ENOG4105C8K Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA (By similarity) Spica_2868 ENOG4105C24 DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings (By similarity) Spica_2869 ENOG4105C7D DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings (By similarity) Spica_R0030 ENOG4105CFP Peptidase U32