locus-tag	class	description
TWT_002	ENOG4105CZ8	DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped onto DNA, it slides freely (bidirectional and ATP- independent) along duplex DNA (By similarity)
TWT_005	ENOG4105C7D	DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings (By similarity)
TWT_006	ENOG4105C24	DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings (By similarity)
TWT_008	ENOG4105FJ0	thiamine
TWT_009	ENOG4107U91	Rhomboid family
TWT_012	ENOG4108V0P	LemA family
TWT_013	ENOG4105D0M	Protease HtpX homolog
TWT_017	ENOG4107QSU	Phosphoglucomutase phosphomannomutase alpha beta alpha domain
TWT_020	ENOG4108JIK	carboxylase
TWT_023	ENOG4105D8K	carboxyl transferase
TWT_031	ENOG4108URP	Dtdp-4-dehydrorhamnose 3,5-epimerase
TWT_034	ENOG4105EY8	ABC transporter
TWT_038	ENOG4106N3V	dolichyl-phosphate beta-D-mannosyltransferase (EC 2.4.1.83)
TWT_047	ENOG4105CK6	Two component transcriptional regulator (Winged helix family
TWT_050	ENOG4105EVN	Prephenate dehydrogenase
TWT_051	ENOG4105DKZ	GTPase that plays an essential role in the late steps of ribosome biogenesis (By similarity)
TWT_052	ENOG4105E4F	Isochorismate synthase
TWT_058	ENOG4105E0A	ABC, transporter
TWT_059	ENOG4105TVJ	transport system, permease
TWT_060	ENOG4105DQ5	transport system permease protein
TWT_062	ENOG4105DK7	lysyl-tRNA synthetase
TWT_064	ENOG4105CQX	Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol (By similarity)
TWT_065	ENOG4105C2Z	ATP-dependent CLP protease ATP-binding subunit
TWT_068	ENOG4105C4A	Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-carboxylate (SEPHCHC) (By similarity)
TWT_071	ENOG4107QIH	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates (By similarity)
TWT_072	ENOG4105D27	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates (By similarity)
TWT_074	ENOG4105K5Y	Binds to Cpn60 in the presence of Mg-ATP and suppresses the ATPase activity of the latter (By similarity)
TWT_075	ENOG4105CP4	Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate- limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth (By similarity)
TWT_076	ENOG4107QVP	Dehydrogenase
TWT_079	ENOG4105CM0	Catalyzes the synthesis of GMP from XMP (By similarity)
TWT_083	ENOG4108V3E	phosphoribosylglycinamide formyltransferase
TWT_084	ENOG4105DC1	bifunctional purine biosynthesis protein purh
TWT_087	ENOG4105D0T	Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs (By similarity)
TWT_088	ENOG4105DZP	Membrane-associated zinc metalloprotease
TWT_089	ENOG4105CEA	Catalyzes the NADP-dependent rearrangement and reduction of 1-deoxy-D-xylulose-5-phosphate (DXP) to 2-C-methyl-D-erythritol 4-phosphate (MEP) (By similarity)
TWT_090	ENOG4108ZF3	Phosphatase
TWT_094	ENOG4105CJ1	ABC, transporter
TWT_097	ENOG4105C13	Site-specific tyrosine recombinase, which acts by catalyzing the cutting and rejoining of the recombining DNA molecules. The XerC-XerD complex is essential to convert dimers of the bacterial chromosome into monomers to permit their segregation at cell division. It also contributes to the segregational stability of plasmids (By similarity)
TWT_100	ENOG4105C8D	Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen (By similarity)
TWT_102	ENOG4105C4F	May be involved in recombinational repair of damaged DNA (By similarity)
TWT_103	ENOG4105F91	Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus (By similarity)
TWT_105	ENOG4105K9Q	Binds to the 23S rRNA (By similarity)
TWT_107	ENOG4107YH6	single-stranded DNA-binding protein
TWT_108	ENOG410829C	Binds together with S18 to 16S ribosomal RNA (By similarity)
TWT_109	ENOG4105C8V	SAICAR synthetase
TWT_110	ENOG4107SIQ	Naphthoate synthase
TWT_116	ENOG4108426	dNA-binding protein
TWT_120	ENOG4105CGV	This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis (By similarity)
TWT_121	ENOG4105FF9	glycosyl transferase, family 39
TWT_122	ENOG4105EIP	mannose-6-phosphate isomerase
TWT_123	ENOG4105K51	Acts as a transcriptional regulator. Probably redox- responsive. The apo- but not holo-form probably binds DNA (By similarity)
TWT_127	ENOG4107QKV	phosphomannomutase
TWT_135	ENOG4105CBI	The glycine cleavage system catalyzes the degradation of glycine. The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor
TWT_139	ENOG4108JJB	ABC transporter
TWT_141	ENOG4105C2T	Binding-protein-dependent transport systems, inner membrane component
TWT_144	ENOG4108UM5	This protein is one of the early assembly proteins of the 50S ribosomal subunit, although it is not seen to bind rRNA by itself. It is important during the early stages of 50S assembly (By similarity)
TWT_147	ENOG4105CS9	pantothenic acid kinase
TWT_152	ENOG4105C6U	Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis- dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) (By similarity)
TWT_154	ENOG4105F0N	pyrophosphate phospho-hydrolase
TWT_157	ENOG4107QHU	One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex (By similarity)
TWT_162	ENOG4107QYE	Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation (By similarity)
TWT_166	ENOG4108YZX	Binds directly to 23S ribosomal RNA and is necessary for the in vitro assembly process of the 50S ribosomal subunit. It is not involved in the protein synthesizing functions of that subunit (By similarity)
TWT_167	ENOG4105CSS	phenylalanyl-tRNA synthetase (alpha subunit)
TWT_168	ENOG4105C6A	phenylalanyl-tRNA synthetase (beta subunit)
TWT_175	ENOG4105CQU	Part of the ABC transporter complex LolCDE involved in the translocation of mature outer membrane-directed lipoproteins, from the inner membrane to the periplasmic chaperone, LolA. Responsible for the formation of the LolA-lipoprotein complex in an ATP-dependent manner (By similarity)
TWT_177	ENOG4108M15	ABC transporter
TWT_183	ENOG4105C90	Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys- tRNA(Pro) is not edited by ProRS (By similarity)
TWT_187	ENOG4105C5T	Phosphoribosyl pyrophosphate synthase
TWT_188	ENOG4105CAJ	Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP- GlcNAc). The C-terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N-acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5- monophosphate (from uridine 5-triphosphate), a reaction catalyzed by the N-terminal domain (By similarity)
TWT_196	ENOG4105CXT	aspartate transcarbamylase
TWT_198	ENOG4105CG1	Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA (By similarity)
TWT_199	ENOG4105CAK	Cell division ATP-binding protein ftsE
TWT_201	ENOG4108UH4	Binds specifically to the SsrA RNA (tmRNA) and is required for stable association of SsrA with ribosomes (By similarity)
TWT_203	ENOG4105D1X	Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits (By similarity)
TWT_204	ENOG4105C01	Dihydroxy-acid dehydratase
TWT_205	ENOG4105C7K	acetolactate synthase
TWT_206	ENOG4108ZP8	Acetolactate synthase small subunit
TWT_207	ENOG4105C6M	Alpha-keto-beta-hydroxylacyl reductoisomerase
TWT_208	ENOG4105CM2	brancheD-chain amino acid aminotransferase
TWT_210	ENOG4105C3M	thioredoxin reductase
TWT_210	ENOG4105C3M	thioredoxin reductase
TWT_211	ENOG4107ZYJ	bioY protein
TWT_213	ENOG4105DKQ	ROK family
TWT_215	ENOG4105C7V	phospho-2-dehydro-3-deoxyheptonate aldolase
TWT_217	ENOG4105EAH	synthase
TWT_219	ENOG4108V6E	Cell division protein mraZ
TWT_222	ENOG4105CJN	penicillin-binding protein
TWT_225	ENOG4107EEN	Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan (By similarity)
TWT_226	ENOG4105DMZ	Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) (By similarity)
TWT_227	ENOG4105CNI	cell division protein FtsW
TWT_229	ENOG4105DFU	Cell wall formation (By similarity)
TWT_238	ENOG4107QYT	Catalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor (By similarity)
TWT_241	ENOG4105C9Y	Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bacterial cell walls (By similarity)
TWT_243	ENOG4105CV4	Subunits I and II form the functional core of the enzyme complex. Electrons originating in cytochrome c are transferred via heme a and Cu(A) to the binuclear center formed by heme a3 and Cu(B) (By similarity)
TWT_244	ENOG4105BZ9	Cytochrome C oxidase, subunit I
TWT_246	ENOG4105CCU	oxidase (Subunit III)
TWT_247	ENOG4107UDY	cytochrome C
TWT_248	ENOG4107EUI	c reductase, iron-sulfur
TWT_249	ENOG4105DJ5	Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis (By similarity)
TWT_255	ENOG4105DAQ	Component of the pyruvate dehydrogenase (PDH) complex, that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2) (By similarity)
TWT_257	ENOG4105EGD	dinuclear metal center protein, YbgI family
TWT_260	ENOG4105CCG	Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate (By similarity)
TWT_262	ENOG4105C22	threonyL-tRNA synthetase
TWT_264	ENOG4105CD9	Involved in the production of pyridoxal phosphate, probably by incorporating ammonia into the pyridine ring (By similarity)
TWT_265	ENOG4108UHX	Involved in the hydrolysis of glutamine to glutamate and ammonia. Channels an ammonia molecule to PdxS (By similarity)
TWT_266	ENOG4105CDY	transcriptional regulatory protein
TWT_267	ENOG4105NHU	Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group (By similarity)
TWT_268	ENOG4105KA4	The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing. RuvA stimulates, in the presence of DNA, the weak ATPase activity of RuvB (By similarity)
TWT_272	ENOG4107RTU	Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. SecDF uses the proton motive force (PMF) to complete protein translocation after the ATP-dependent function of SecA (By similarity)
TWT_275	ENOG4105CWR	In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance (By similarity)
TWT_278	ENOG4105C4S	Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- translocation proceeds from a post-translocation (POST) complex to a pre-translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP-dependent manner (By similarity)
TWT_280	ENOG4105BZ5	ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins (By similarity)
TWT_281	ENOG4105QF0	Specifically methylates the N3 position of the uracil ring of uridine 1498 (m3U1498) in 16S rRNA. Acts on the fully assembled 30S ribosomal subunit (By similarity)
TWT_285	ENOG4106DBE	Involved in DNA repair and RecF pathway recombination (By similarity)
TWT_286	ENOG4105CR3	Catalyzes the condensation of isopentenyl diphosphate (IPP) with allylic pyrophosphates generating different type of terpenoids (By similarity)
TWT_287	ENOG4105C4Q	deoxyguanosinetriphosphate triphosphohydrolase-like protein
TWT_289	ENOG4105K4H	Nlp p60 protein
TWT_290	ENOG4105CA9	Pyruvate kinase
TWT_292	ENOG4105C2M	dna polymerase I
TWT_294	ENOG4108ZQD	Catalyzes the phosphorylation of the 3'-hydroxyl group of dephosphocoenzyme A to form coenzyme A (By similarity)
TWT_296	ENOG4105C5U	The UvrABC repair system catalyzes the recognition and processing of DNA lesions. UvrA is an ATPase and a DNA-binding protein. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. When the presence of a lesion has been verified by UvrB, the UvrA molecules dissociate (By similarity)
TWT_297	ENOG4105CII	The UvrABC repair system catalyzes the recognition and processing of DNA lesions. UvrC both incises the 5' and 3' sides of the lesion. The N-terminal half is responsible for the 3' incision and the C-terminal half is responsible for the 5' incision (By similarity)
TWT_299	ENOG4105CK4	Destroys radicals which are normally produced within the cells and which are toxic to biological systems (By similarity)
TWT_300	ENOG4105C17	glyceraldehyde-3-phosphate dehydrogenase
TWT_301	ENOG4105BZA	Phosphoglycerate kinase
TWT_302	ENOG4105CP7	Triose-phosphate isomerase
TWT_305	ENOG4105C6U	Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis- dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) (By similarity)
TWT_307	ENOG4105F0N	pyrophosphate phospho-hydrolase
TWT_308	ENOG4107XU2	6-phosphogluconolactonase (EC 3.1.1.31)
TWT_309	ENOG4107TBN	Transfers the N-acyl diglyceride group on what will become the N-terminal cysteine of membrane lipoproteins (By similarity)
TWT_310	ENOG4108Z02	Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions (By similarity)
TWT_313	ENOG4105CHR	reductase
TWT_315	ENOG4105DWR	Catalyzes the dephosphorylation of undecaprenyl diphosphate (UPP). Confers resistance to bacitracin (By similarity)
TWT_320	ENOG4105DCX	Part of the twin-arginine translocation (Tat) system that transports large folded proteins containing a characteristic twin-arginine motif in their signal peptide across membranes. Together with TatB, TatC is part of a receptor directly interacting with Tat signal peptides (By similarity)
TWT_321	ENOG4107EXU	Short-chain dehydrogenase reductase Sdr
TWT_325	ENOG4105F8V	Hydrolase, tatD family
TWT_326	ENOG4105CPQ	Dehydrogenase
TWT_328	ENOG4105DFW	ABC transporter
TWT_329	ENOG4108HWW	ABC transporter
TWT_331	ENOG4105CR3	Catalyzes the condensation of isopentenyl diphosphate (IPP) with allylic pyrophosphates generating different type of terpenoids (By similarity)
TWT_333	ENOG4105VG1	fes assembly suf system protein
TWT_334	ENOG4105C3K	feS assembly ATPase SufC
TWT_337	ENOG4105DIW	FeS assembly protein SUFB
TWT_338	ENOG4107SGF	Catalyzes the oxidation of the C8 methyl side group on heme O porphyrin ring into a formyl group (By similarity)
TWT_341	ENOG4107QQX	Transaldolase is important for the balance of metabolites in the pentose-phosphate pathway (By similarity)
TWT_342	ENOG4105C89	phosphohexose isomerase
TWT_349	ENOG4108VJ1	Transcriptional regulator (CarD family
TWT_351	ENOG4105C75	arginyL-tRNA synthetase
TWT_352	ENOG4105BZY	Part of the ABC transporter complex PstSACB involved in phosphate import. Responsible for energy coupling to the transport system (By similarity)
TWT_355	ENOG4107QJG	Part of the ABC transporter complex PstSACB involved in phosphate import (By similarity)
TWT_358	ENOG4105CAE	Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by (I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-GTP (By similarity)
TWT_360	ENOG4105CPH	Catalyzes the formation of S-adenosylmethionine from methionine and ATP
TWT_362	ENOG4105MTQ	Promotes RNA polymerase assembly. Latches the N- and C- terminal regions of the beta' subunit thereby facilitating its interaction with the beta and alpha subunits (By similarity)
TWT_364	ENOG4105SKN	orotidine 5''-phosphate decarboxylase
TWT_365	ENOG4105CU6	carbamoyl-phosphate synthetase ammonia chain
TWT_366	ENOG4105C1M	carbamoyl-phosphate synthetase glutamine chain
TWT_367	ENOG4105CD3	dihydroorotase
TWT_369	ENOG4108Z38	Catalyzes a trans-dehydration via an enolate intermediate (By similarity)
TWT_370	ENOG4105D49	3-dehydroquinate synthase
TWT_371	ENOG4105KHV	Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate (By similarity)
TWT_373	ENOG4105E2X	shikimate dehydrogenase
TWT_384	ENOG4105KQC	Competence protein
TWT_385	ENOG4105C8T	Leucyl-tRNA synthetase
TWT_386	ENOG4105CRQ	synthase (Component I)
TWT_394	ENOG4105ZIE	Exonuclease involved in the 3' processing of various precursor tRNAs. Initiates hydrolysis at the 3'-terminus of an RNA molecule and releases 5'-mononucleotides (By similarity)
TWT_398	ENOG4105MC2	DSBA oxidoreductase
TWT_406	ENOG4108RBP	3'-to-5' exoribonuclease specific for small oligoribonucleotides (By similarity)
TWT_408	ENOG4105BZ1	(ABC) transporter
TWT_411	ENOG4105CHT	Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln) (By similarity)
TWT_412	ENOG4105C3P	Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln) (By similarity)
TWT_413	ENOG4105VB5	Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln) (By similarity)
TWT_414	ENOG4105C77	DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA (By similarity)
TWT_415	ENOG4105CCJ	Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s(2)U34 (By similarity)
TWT_416	ENOG4107QI6	Cysteine desulfurase
TWT_420	ENOG4105PGM	cation diffusion facilitator family transporter
TWT_421	ENOG4105C62	Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction (By similarity)
TWT_423	ENOG4105KNM	Produces ATP from ADP in the presence of a proton gradient across the membrane (By similarity)
TWT_425	ENOG4105J80	Produces ATP from ADP in the presence of a proton gradient across the membrane. The gamma chain is believed to be important in regulating ATPase activity and the flow of protons through the CF(0) complex (By similarity)
TWT_430	ENOG4107UH5	it plays a direct role in the translocation of protons across the membrane (By similarity)
TWT_432	ENOG4105ESV	Glycosyl transferase, family 4
TWT_434	ENOG4105EQY	Methylates the class 1 translation termination release factors RF1 PrfA and RF2 PrfB on the glutamine residue of the universally conserved GGQ motif (By similarity)
TWT_435	ENOG4105C8K	Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA (By similarity)
TWT_437	ENOG4105D5I	Catalyzes the ATP-dependent phosphorylation of L- homoserine to L-homoserine phosphate (By similarity)
TWT_438	ENOG4107R5I	Catalyzes the gamma-elimination of phosphate from L- phosphohomoserine and the beta-addition of water to produce L- threonine (By similarity)
TWT_439	ENOG4105D6E	homoserine dehydrogenase
TWT_441	ENOG4105CJ9	Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions (By similarity)
TWT_445	ENOG4105KNE	Phosphatidate cytidylyltransferase
TWT_448	ENOG4105CU7	Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome (By similarity)
TWT_452	ENOG4108IYT	Site-specific tyrosine recombinase, which acts by catalyzing the cutting and rejoining of the recombining DNA molecules. The XerC-XerD complex is essential to convert dimers of the bacterial chromosome into monomers to permit their segregation at cell division. It also contributes to the segregational stability of plasmids (By similarity)
TWT_453	ENOG4105C79	DNA protecting protein DprA
TWT_454	ENOG4105C0P	Mg chelatase subunit ChlI
TWT_455	ENOG41080R2	UPF0102 protein
TWT_458	ENOG4105D6X	Specifically methylates guanosine-37 in various tRNAs (By similarity)
TWT_459	ENOG4107XUM	An accessory protein needed during the final step in the assembly of 30S ribosomal subunit, possibly for assembly of the head region. Probably interacts with S19. Essential for efficient processing of 16S rRNA. May be needed both before and after RbfA during the maturation of 16S rRNA. It has affinity for free ribosomal 30S subunits but not for 70S ribosomes (By similarity)
TWT_460	ENOG41082KU	UPF0109 protein
TWT_461	ENOG4105K5M	30s ribosomal protein S16
TWT_462	ENOG4105CB9	Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds to the hydrophobic signal sequence of the ribosome-nascent chain (RNC) as it emerges from the ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY
TWT_463	ENOG4108BDP	Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate (By similarity)
TWT_469	ENOG4108Z1W	Catalyzes the reversible adenylation of nicotinate mononucleotide (NaMN) to nicotinic acid adenine dinucleotide (NaAD) (By similarity)
TWT_471	ENOG4105C9R	An essential GTPase which binds GTP, GDP and possibly (p)ppGpp with moderate affinity, with high nucleotide exchange rates and a fairly low GTP hydrolysis rate (By similarity). It may play a role in control of the cell cycle, stress response, ribosome biogenesis and in those bacteria that undergo differentiation, in morphogenesis control
TWT_472	ENOG4105K46	50S ribosomal protein l27
TWT_473	ENOG410826X	This protein binds to 23S rRNA in the presence of protein L20 (By similarity)
TWT_474	ENOG4105C03	ribonuclease
TWT_475	ENOG4108UGX	Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate (By similarity)
TWT_476	ENOG4105DPM	folylpolyglutamate synthase Dihydrofolate synthase
TWT_478	ENOG4105CA4	amino acids such as threonine, to avoid such errors, it has a posttransfer editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA-dependent manner (By similarity)
TWT_479	ENOG4105CHN	ATP-dependent specificity component of the Clp protease. It directs the protease to specific substrates. Can perform chaperone functions in the absence of ClpP (By similarity)
TWT_480	ENOG4105CCQ	Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins (By similarity)
TWT_483	ENOG4105DEA	Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation
TWT_491	ENOG4105C24	DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings (By similarity)
TWT_492	ENOG4105C7Q	Catalyzes the oxidative decarboxylation of 6- phosphogluconate to ribulose 5-phosphate and CO(2), with concomitant reduction of NADP to NADPH (By similarity)
TWT_494	ENOG4105C7D	DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings (By similarity)
TWT_495	ENOG4105CJ4	Catalyzes the interconversion of L-alanine and D- alanine. May also act on other amino acids (By similarity)
TWT_496	ENOG4105CJ4	Catalyzes the interconversion of L-alanine and D- alanine. May also act on other amino acids (By similarity)
TWT_499	ENOG4105DG1	Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released (By similarity)
TWT_500	ENOG4105C38	Glycyl-tRNA synthetase beta subunit
TWT_505	ENOG4105CPF	Cell wall formation (By similarity)
TWT_506	ENOG4105CSF	NADPH-dependent glycerol-3-phosphate dehydrogenase
TWT_507	ENOG4107RIS	Acyl-transferase
TWT_508	ENOG4105CDF	Cell wall formation. Adds enolpyruvyl to UDP-N- acetylglucosamine (By similarity)
TWT_509	ENOG4105DU1	integral membrane protein, terc
TWT_510	ENOG4105C0B	DNA polymerase III (alpha subunit)
TWT_512	ENOG4107ZIW	This protein specifically catalyzes the removal of signal peptides from prolipoproteins (By similarity)
TWT_513	ENOG4107VQS	DivIVA family
TWT_515	ENOG4105CDK	Essential cell division protein that forms a contractile ring structure (Z ring) at the future cell division site. The regulation of the ring assembly controls the timing and the location of cell division. One of the functions of the FtsZ ring is to recruit other cell division proteins to the septum to produce a new cell wall between the dividing cells. Binds GTP and shows GTPase activity (By similarity)
TWT_519	ENOG4105C4R	Atp-dependent dna helicase
TWT_522	ENOG4107T6F	Xaa-Pro aminopeptidase
TWT_523	ENOG4105CJW	magnesium transporter
TWT_526	ENOG4105DI7	Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs (By similarity)
TWT_533	ENOG4105F0I	Aminotransferase
TWT_535	ENOG4105CGG	The central subunit of the protein translocation channel SecYEG. Consists of two halves formed by TMs 1-5 and 6-10. These two domains form a lateral gate at the front which open onto the bilayer between TMs 2 and 7, and are clamped together by SecE at the back. The channel is closed by both a pore ring composed of hydrophobic SecY resides and a short helix (helix 2A) on the extracellular side of the membrane which forms a plug. The plug probably moves laterally to allow the channel to open. The ring and the pore may move independently (By similarity)
TWT_536	ENOG4108UZ0	Binds to the 23S rRNA (By similarity)
TWT_537	ENOG410849U	50S ribosomal protein L30
TWT_546	ENOG41082SA	50s ribosomal protein l29
TWT_552	ENOG4105KZR	One of the early assembly proteins it binds 23S rRNA. One of the proteins that surrounds the polypeptide exit tunnel on the outside of the ribosome. Forms the main docking site for trigger factor binding to the ribosome (By similarity)
TWT_553	ENOG4106U5A	One of the primary rRNA binding proteins, this protein initially binds near the 5'-end of the 23S rRNA. It is important during the early stages of 50S assembly. It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome (By similarity)
TWT_556	ENOG4105CCP	Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Acts as a receptor for the complex formed by the signal recognition particle (SRP) and the ribosome-nascent chain (RNC)
TWT_557	ENOG4108IV0	ABC, transporter
TWT_558	ENOG4105C0Z	ABC transporter
TWT_559	ENOG4105CF8	branched-chain amino acid ABC transporter, permease
TWT_560	ENOG4105C32	Amino acid ABC transporter (Permease)
TWT_571	ENOG4108VQJ	O-methyltransferase
TWT_575	ENOG4108Z4U	Ferredoxin
TWT_581	ENOG4105DTN	riboflavin biosynthesis protein ribF
TWT_584	ENOG4108IUZ	ABC transporter
TWT_586	ENOG4105EEI	dihydropteroate synthase
TWT_587	ENOG4105EUJ	GTP cyclohydrolase i
TWT_588	ENOG4105C3H	Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins. Plays a role in the quality control of integral membrane proteins (By similarity)
TWT_589	ENOG4108UGV	hypoxanthine phosphoribosyltransferase
TWT_590	ENOG4107RD1	Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine (By similarity)
TWT_593	ENOG4108097	ATP-binding protein
TWT_595	ENOG4105CI3	gtp-binding protein typa
TWT_600	ENOG4105BZ1	(ABC) transporter
TWT_601	ENOG4105BZ1	(ABC) transporter
TWT_605	ENOG4105CTR	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol (By similarity)
TWT_606	ENOG4105C46	Catalyzes the first step in hexosamine metabolism, converting fructose-6P into glucosamine-6P using glutamine as a nitrogen source (By similarity)
TWT_609	ENOG4105DKX	Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A) (By similarity)
TWT_610	ENOG4105CIW	Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine (By similarity)
TWT_614	ENOG4107QZI	cell division protein FtsK
TWT_615	ENOG4105CN5	Metallo-Beta-Lactamase
TWT_616	ENOG4107V6K	Catalyzes the formation of dTMP and tetrahydrofolate from dUMP and methylenetetrahydrofolate (By similarity)
TWT_618	ENOG4105KRM	Acts as a transcriptional regulator. Probably redox- responsive. The apo- but not holo-form probably binds DNA (By similarity)
TWT_621	ENOG4108V82	Pyrophosphatase that hydrolyzes non-canonical purine nucleotides such as XTP and ITP dITP to their respective monophosphate derivatives. Might exclude non-canonical purines from DNA precursor pool, thus preventing their incorporation into DNA and avoiding chromosomal lesions (By similarity)
TWT_625	ENOG4105C0H	protease
TWT_630	ENOG4108JIJ	Catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second (By similarity)
TWT_631	ENOG4107QJE	Chromosome Partitioning Protein
TWT_634	ENOG4105CN0	Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate (By similarity)
TWT_636	ENOG4106H78	methyltransferase
TWT_638	ENOG4105D43	The glycine cleavage system catalyzes the degradation of glycine (By similarity)
TWT_639	ENOG4105C3G	gtp-binding protein
TWT_642	ENOG4105C48	Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) (By similarity)
TWT_644	ENOG4105YX8	Bidirectionally degrades single-stranded DNA into large acid-insoluble oligonucleotides, which are then degraded further into small acid-soluble oligonucleotides (By similarity)
TWT_645	ENOG4105DJB	Major Facilitator Superfamily
TWT_651	ENOG4108ZPD	The natural substrate for this enzyme may be peptidyl- tRNAs which drop off the ribosome during protein synthesis (By similarity)
TWT_654	ENOG4107XT7	This is one of the proteins that binds to the 5S RNA in the ribosome where it forms part of the central protuberance (By similarity)
TWT_661	ENOG41080FJ	Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell (By similarity)
TWT_663	ENOG4105DIK	(ABC) transporter
TWT_664	ENOG4105CC5	ABC transporter
TWT_675	ENOG4105CEJ	Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome (By similarity)
TWT_680	ENOG4107YD5	Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by cleavage factors such as GreA or GreB allows the resumption of elongation from the new 3'terminus. GreA releases sequences of 2 to 3 nucleotides (By similarity)
TWT_681	ENOG4105C7B	Threonine dehydratase
TWT_682	ENOG4105E05	Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides (By similarity)
TWT_683	ENOG4105BZH	Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides (By similarity)
TWT_686	ENOG4105C31	Tryptophanyl-tRNA synthetase
TWT_688	ENOG4108R6K	riboflavin synthase, subunit alpha
TWT_689	ENOG4105C66	Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate (By similarity)
TWT_692	ENOG4108RWI	nicotinamidase
TWT_694	ENOG4105C6E	dna polymerase iii
TWT_696	ENOG4105C73	Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity)
TWT_702	ENOG4105KME	endoribonuclease L-psp
TWT_705	ENOG4105BZ4	penicillin-binding protein
TWT_708	ENOG4105CFH	Aspartokinase
TWT_709	ENOG4105F3K	May play a role in DNA repair. It seems to be involved in an RecBC-independent recombinational process of DNA repair. It may act with RecF and RecO (By similarity)
TWT_711	ENOG4105KBC	Seems to be the binding site for several of the factors involved in protein synthesis and appears to be essential for accurate translation (By similarity)
TWT_715	ENOG4105E5V	Participates in transcription elongation, termination and antitermination (By similarity)
TWT_716	ENOG410683Z	Preprotein translocase SecE subunit
TWT_718	ENOG4107R4N	Cell wall formation (By similarity)
TWT_723	ENOG4105CBA	glutamine phosphoribosylpyrophosphate amidotransferase
TWT_724	ENOG4105CXB	phosphoribosylaminoimidazole synthetase
TWT_725	ENOG4105C12	Phosphoribosylglycinamide synthetase
TWT_726	ENOG4105CGR	Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec) (By similarity)
TWT_732	ENOG4105D6W	Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps (By similarity)
TWT_734	ENOG4107JTW	chlorite dismutase
TWT_737	ENOG4107TCW	O-succinylbenzoate synthase
TWT_738	ENOG4105CDU	Replicative dna helicase
TWT_743	ENOG41065E1	Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) (By similarity)
TWT_744	ENOG4105CGK	Exodeoxyribonuclease III
TWT_746	ENOG4105C2Z	ATP-dependent CLP protease ATP-binding subunit
TWT_747	ENOG4105KNI	merR family transcriptional Regulator
TWT_749	ENOG4108093	Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with DnaK and GrpE. It is the nucleotide exchange factor for DnaK and may function as a thermosensor. Unfolded proteins bind initially to DnaJ
TWT_751	ENOG4108Z6B	deaminase
TWT_754	ENOG4105C5S	asparaginyl-tRNA synthetase
TWT_755	ENOG4108RH7	phosphoglycerate mutase
TWT_756	ENOG4105K63	Thioredoxin
TWT_757	ENOG4107QIV	Cytochrome c biogenesis protein
TWT_758	ENOG4105EBH	Required during biogenesis of c-type cytochromes (cytochrome c6 and cytochrome f) at the step of heme attachment (By similarity)
TWT_759	ENOG4105CRE	cytochrome C
TWT_761	ENOG4105CC5	ABC transporter
TWT_763	ENOG4105CKH	Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation (By similarity)
TWT_765	ENOG4106N3V	dolichyl-phosphate beta-D-mannosyltransferase (EC 2.4.1.83)
TWT_769	ENOG4105C9K	Endothelin-converting enzyme 1
TWT_770	ENOG4108VXP	Specifically catalyzes the dephosphorylation of 2- phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stress (By similarity)
TWT_776	ENOG4105CJN	penicillin-binding protein
TWT_777	ENOG4107TPY	serine threonine protein kinase
TWT_778	ENOG4105D9P	serine threonine protein kinase
TWT_786	ENOG4105DRC	Na( ) H( ) antiporter that extrudes sodium in exchange for external protons (By similarity)
TWT_788	ENOG4107RN0	branched-chain alpha-keto acid dehydrogenase subunit E2
TWT_789	ENOG4105CPP	Dehydrogenase, E1 component
TWT_795	ENOG4105D21	Phosphoribosylformylglycinamidine synthase I
TWT_797	ENOG4107R5M	Catalyzes the transfer of a N-acetyl-glucosamine moiety to 1D-myo-inositol 3-phosphate to produce 1D-myo-inositol 2- acetamido-2-deoxy-glucopyranoside 3-phosphate in the mycothiol biosynthesis pathway (By similarity)
TWT_798	ENOG4108KZ5	Catalyzes the deacetylation of 1D-myo-inositol 2- acetamido-2-deoxy-alpha-D-glucopyranoside (GlcNAc-Ins) in the mycothiol biosynthesis pathway (By similarity)
TWT_800	ENOG4105D4J	Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate
TWT_801	ENOG4105FA0	parb-like partition protein
TWT_802	ENOG4107QJE	Chromosome Partitioning Protein
TWT_803	ENOG4105C59	p-type ATPase
TWT_805	ENOG4105DHW	Required for the insertion and or proper folding and or complex formation of integral membrane proteins into the membrane. Involved in integration of membrane proteins that insert both dependently and independently of the Sec translocase complex, as well as at least some lipoproteins
TWT_808	ENOG41084CH	50S ribosomal protein l34