LOCUS MG592402 10636 bp DNA linear VRL 24-JAN-2018 DEFINITION Vibrio phage 1.020.O._10N.222.48.A2, partial genome. ACCESSION MG592402 VERSION MG592402.1 DBLINK BioProject: PRJNA328102 KEYWORDS . SOURCE Vibrio phage 1.020.O._10N.222.48.A2 ORGANISM Vibrio phage 1.020.O._10N.222.48.A2 Viruses; dsDNA viruses, no RNA stage; unclassified dsDNA viruses. REFERENCE 1 (bases 1 to 10636) AUTHORS Kauffman,K.M., Hussain,F.A., Yang,J., Arevalo,P., Brown,J.M., Chang,W.K., VanInsberghe,D., Elsherbini,J., Cutler,M.B., Kelly,L. and Polz,M.F. TITLE A major lineage of nontailed dsDNA viruses as unrecognized killers of marine bacteria JOURNAL Unpublished REFERENCE 2 (bases 1 to 10636) AUTHORS Kauffman,K.M., Hussain,F.A., Yang,J., Arevalo,P., Brown,J.M., Chang,W.K., VanInsberghe,D., Elsherbini,J., Cutler,M.B., Kelly,L. and Polz,M.F. TITLE Direct Submission JOURNAL Submitted (29-NOV-2017) Department of Civil and Environmental Engineering, Massachusetts Institute of Technology, 15 Vassar Street, Bldg. 48-417, Cambridge, MA 02139, USA FEATURES Location/Qualifiers source 1..10636 /organism="Vibrio phage 1.020.O._10N.222.48.A2" /mol_type="genomic DNA" /isolation_source="iron chloride precipitation of 0.2um-filtered seawater, resuspended in oxalate solution" /host="Vibrio tasmaniensis" /db_xref="taxon:1881450" /lab_host="10N.222.48.A2" /country="USA: Massachusetts, Nahant, Canoe Cove" /lat_lon="42.419 N 70.906 W" /collection_date="2010-08-10" /collected_by="Kathryn Kauffman" gene complement(127..369) /locus_tag="NVP1020O_01" CDS complement(127..369) /locus_tag="NVP1020O_01" /note="NVP1020O_01" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AUR82043.1" /translation="MSLNFPESRRQMNMANRETHVIRVTNANKTLNIVVKNGDESMTV KPMMFFEIATDNPKRFWIDANVLAIQNVTVRVWELD" gene complement(374..559) /locus_tag="NVP1020O_02" CDS complement(374..559) /locus_tag="NVP1020O_02" /note="NVP1020O_02" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AUR82044.1" /translation="MLARIKNLTSTSIIILNVALNRHLEITEGETKLTEIIDFEDLVI IKYKLCETHLIEMDIVR" gene complement(498..752) /locus_tag="NVP1020O_03" CDS complement(498..752) /locus_tag="NVP1020O_03" /note="NVP1020O_03" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AUR82045.1" /translation="MSRYVRSTHYGALYLVTNWTADLDETIISYLDVVWGGHYRLDSQ SNQHEKAIQLFWNEEEGNLTHVSENKKPNIYLNHNSKRGS" gene complement(755..1291) /locus_tag="NVP1020O_04" CDS complement(755..1291) /locus_tag="NVP1020O_04" /note="NVP1020O_04" /codon_start=1 /transl_table=11 /product="TMhelix containing protein" /protein_id="AUR82046.1" /translation="MSDFYIYVIVAIAILTCASICAKAGSYGVALVLVHAVCMISPAD ASENKFKAFMGDAYSFHFNRSFDYNEVHKTYGLEYQITPQWGINTTSFTNSFSERSNS LAVVYTWKRDELLSNVHWSAGVQTGIATGYEKAKYNAGGVAPIVAPFVDVGVFESVGA RFSIWNAYVANVSFYLEF" gene complement(1489..1821) /locus_tag="NVP1020O_05" CDS complement(1489..1821) /locus_tag="NVP1020O_05" /note="NVP1020O_05" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AUR82047.1" /translation="MSKSTQPTFKRVAAVSLPLLSLDYGKDGAAKSHFIKIEKEFAEH VSAKEDAKPDMMKSTVTELESGEQFDIYLHKGLAAKFQSGKAYEVVTRKVQGKRFPEH TIYEIEVA" gene complement(1934..3529) /locus_tag="NVP1020O_06" CDS complement(1934..3529) /locus_tag="NVP1020O_06" /note="NVP1020O_06; aclame_best_match:protein:vir:2124; tara_best_match:OM-RGC.v1.001450554; cvp_best_match:RF_PEP_9626351; pfam:PF03175|pog:POG0334" /codon_start=1 /transl_table=11 /product="DNA polymerase type B" /protein_id="AUR82048.1" /translation="MGKKRNDLESIGAADCETDPFKYGRVPKPFLWGLYINEQYYEFE KTIDFVNFVRDEEWTIYAHNGGRFDWHFVLWECEIKPDIKIIAGRIAEFKIGKCTFRD SYNILPIPLAAYQKDEISYDLFEKEERYKPENWKAIRDYLKSDCVYLHELVTRFVTDY GFNITLASTALKVFNNMAGLKTPRTNINYFENLNRFYFGGRVQAFKTGVFEKDFYYVD INSAYPYAMKYPHAYGNKRAKSRRLPEERDPKIFIRLKCQSNGHFPIRTEKGALSFPD DKQVYEFHITGHEYYMAEDLGLLREVEILEVLTFEDEICFGDYVDHFYVMKEEATING DAAKRLFAKLFMNSLYGKYATNPTKYKQYSVQNINTAEEFEYNNPDYECATEFGDNLL YERDLYDDELKFLNVATAASITGYVRAFMMQALCSVKNPYYCDTDSIICEGLGDLEID ATKLGAWDIEAKATFCAIAGKKLYTMRCEDGSTKTASKGARLTHEQLIEVASGGEVLY KSEAPTFSVSRGVRFIERKIRKT" gene complement(3535..4110) /locus_tag="NVP1020O_07" CDS complement(3535..4110) /locus_tag="NVP1020O_07" /note="NVP1020O_07; Putative terminal protein" /codon_start=1 /transl_table=11 /product="putative terminal protein" /protein_id="AUR82049.1" /translation="MKEKEYKELFKALRPFVNFNIDLRKKLSPADKRLITIYGKQFKD IYQGQNIKVFRSSNKDNLKIVQNAYHQTLKTLPRWKVAFVPYNGKQAPNIKVKNGVIK TFNKRSKMKQFIIPIVDQFSFLNNTFNYVTELLEIHQVPPRQKLWVKTGDYLSTESFE AEILAEEMADWVERYENADTFITGFQSAFYR" gene 4124..4858 /locus_tag="NVP1020O_08" CDS 4124..4858 /locus_tag="NVP1020O_08" /note="NVP1020O_08" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AUR82050.1" /translation="MDTSDNHQISDLVSAVSDSTIEDNTTLTENEGSVNFQRIAEIGE VSENPETPQSGEIPRELMFGENGEIRTDADGAQFDPGKHVVDEQGHPKLTAKGKLRKK RGRKSGSLATTTAAQTAQKVAVSEKEKYRVVGAGAANALISVGMMLGGEDFIPIQNQH IDEKKNLEAAFSQWAETQEVEDIPPNLALTIVMGAYLLPRLSMPKQVSKLRLAKEWFK MKFFRKPKAAKVAKSAFDPNKENKEG" gene 4861..5394 /locus_tag="NVP1020O_09" CDS 4861..5394 /locus_tag="NVP1020O_09" /note="NVP1020O_09; tara_best_match:OM-RGC.v1.021972741; InterPro:IPR027417; Putative packaging ATPase" /codon_start=1 /transl_table=11 /product="P-loop containing nucleoside triphosphate hydrolase" /protein_id="AUR82051.1" /translation="MPHCAIVGMTESGKSSLGRTFAKELQKSGIQIIVFDPLGDPEWN NALDLENAFITDNENEFLNVYWNSKNCAVFFDEAGDYATNHNKGMIRTATKGRHWGHS NFYIAQRGNLLARTIRDQCANLYMFTSSKDDAKIYSTEFNDAELLNVPDLPQGTYYTC GRFKKAARHILFKGKSK" gene 5485..5709 /locus_tag="NVP1020O_10" CDS 5485..5709 /locus_tag="NVP1020O_10" /note="NVP1020O_10; aclame_best_match:protein:plasmid:123104; tara_best_match:OM-RGC.v1.008892567; cvp_best_match:RF_PEP_370703038; kegg:none|pfam:PF08017|cog:COG3064|egg:COG3064" /codon_start=1 /transl_table=11 /product="fibrinogen binding protein" /protein_id="AUR82052.1" /translation="MLIKLKRRKLKLQMEIDKKAEAKKQAEAEVKKQAEAEAKKQAEA EAKKQAEAEAKKHAEAEAEVKANSGSDNLE" gene 5947..6072 /locus_tag="NVP1020O_11" CDS 5947..6072 /locus_tag="NVP1020O_11" /note="NVP1020O_11" /codon_start=1 /transl_table=11 /product="TMhelix containing protein" /protein_id="AUR82053.1" /translation="MPKKYMPYAINAVVAGVTAYLITSAVAKQRQVQWYNPTTWF" gene 6124..6969 /locus_tag="NVP1020O_12" CDS 6124..6969 /locus_tag="NVP1020O_12" /note="NVP1020O_12; tara_best_match:OM-RGC.v1.009973837; Double jelly roll capsid protein" /codon_start=1 /transl_table=11 /product="double jelly roll capsid protein" /protein_id="AUR82054.1" /translation="MAFRSNKILPNFNGVGAGQTATLDVPIGSTYHQVAFEIAGVTPA QLTNFKVLLNGIPHIELPSVEILNTLNKFDGRLNSAGVFVLDFERFGLLTRQARELTA IGTGMGDADPNQVRTFQIQMDIDAAATSPSIKATATTSPASMSGLVRKIRLFNRNPTG AGLFEVSDLPKGDMINKVFFHAAASSDIDALSVEIDNYVKFERSAALNSDIQQSDGVR MPAPTMFVFDPTELGYGAEVVDTVYPEGTPRAGQNVSDLRFKLRMAGAQAQLLIVEYL GAVER" gene 7047..7373 /locus_tag="NVP1020O_13" CDS 7047..7373 /locus_tag="NVP1020O_13" /note="NVP1020O_13" /codon_start=1 /transl_table=11 /product="TMhelix containing protein" /protein_id="AUR82055.1" /translation="MNVDSNMFENWFTTTTPQGQQVSLWDNILNAGGQVINSLPDVVD GIIDTKLDAERKTQTEKTENIASTNPNVAGEANSKNPMNNQNMMMGMYVVGGLLATVV LIKVLK" gene 7385..7534 /locus_tag="NVP1020O_14" CDS 7385..7534 /locus_tag="NVP1020O_14" /note="NVP1020O_14" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AUR82056.1" /translation="MPFLLPIAVLGGTGIFAWKAGDAAGNAVETAAKVAAVGGVLYLI YKSKG" gene 7538..7723 /locus_tag="NVP1020O_15" CDS 7538..7723 /locus_tag="NVP1020O_15" /note="NVP1020O_15" /codon_start=1 /transl_table=11 /product="TMhelix containing protein" /protein_id="AUR82057.1" /translation="MQAAGGGGMSAGSSAGAVGGASGSSIGGVNIDLSGKGDMNVTYL VAGGLALGALFIIMSYK" gene 7733..8092 /locus_tag="NVP1020O_16" CDS 7733..8092 /locus_tag="NVP1020O_16" /note="NVP1020O_16" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AUR82058.1" /translation="MQKFEPVSIDEALANMPKSIHRHTDLVKDLIRKGVQHAYKFGEL YVIFEPWENTLMVQCVEGSGLNDAFVKALYDCATNNGFEYIQFVTPHKGLHKMVKSLN PERIEYRYICKLDEVKL" gene 8089..8658 /locus_tag="NVP1020O_17" CDS 8089..8658 /locus_tag="NVP1020O_17" /note="NVP1020O_17" /codon_start=1 /transl_table=11 /product="TMhelix containing protein" /protein_id="AUR82059.1" /translation="MSFSRGGSSKSATTNEQNPITATDNAMVASEGGTITVTDGGAFG LVGDAVDAVTKTFSDMIFSNEKVIGSSIDAVTAATSDAIFSNEKVVGDAMLTNESIAN SAIDAGLEYQKGSNNVITDTLERVFDFVGDTVTKSQDTISSSATALFDSAKSDELNKS QDMQQTIRTLAMYAAVGSVAFITIKYAFK" gene 8669..9343 /locus_tag="NVP1020O_18" CDS 8669..9343 /locus_tag="NVP1020O_18" /note="NVP1020O_18" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AUR82060.1" /translation="MALIRDYHLTEVAPLEAETRVVNVVGNTIVVRRAVDAGAVRVKA YTKDGGALVTDNVLSAGEKIRTAVKFTRLEITNLESTKKHIHITAGEGDFQSDVVEGE VTVLTSDSSPLKVVGQVVTTPSNSTPTDIVGTVITREAKFDNVSRNYAHTSGTPTSIP VPANAVKFGIQNVTAGEGAVYLSGAHVYIQPKQVFSIECSDDLVVTLDQSSHSGTYNV FWVTQP" gene 9353..9871 /locus_tag="NVP1020O_19" CDS 9353..9871 /locus_tag="NVP1020O_19" /note="NVP1020O_19; tara_best_match:OM-RGC.v1.023097787; egg:ENOG4111U4Z" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AUR82061.1" /translation="MEINPLFIVIPIIGIYVMSKQVPSIHPSRGYRNNNPLNIRISSS KWKGEIPHSKNTDGAFEQFYERRFGYRAAAKLILNYNRLYNIFTVEGIINRWAPAVGE TPQGQYYTNHTQQYIDYVCKITGFEPNTLINGSNVGKLVWAMARFENTPPHTDTLEHV NDAVSEFAHINY" gene 9884..10291 /locus_tag="NVP1020O_20" CDS 9884..10291 /locus_tag="NVP1020O_20" /note="NVP1020O_20" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AUR82062.1" /translation="MFLSAPLPVPQEFITRTKVLGAPSVPNAQEIGAPAPKTWVQMSQ IIPATCERLLITVSRAYSSASAETGFIINRASDGLRMYDVNPTSKPNTHDTNTHEIQR PAEGWGALKIYINTSGAGTIRFVIIHQLANLYS" gene 10300..10512 /locus_tag="NVP1020O_21" CDS 10300..10512 /locus_tag="NVP1020O_21" /note="NVP1020O_21" /codon_start=1 /transl_table=11 /product="TMhelix containing protein" /protein_id="AUR82063.1" /translation="MDKKKVTNIILAMVAAGVTAAGATNINFTVTPANDEQKLLECLT VIDGMIKTHSNDLVKVTEILIQGSKP" ORIGIN 1 cgccttttcg ctcgtgggtc acactttaga catttaaatt tgacaaacgt tagaatgtat 61 gaaacgaaaa agccccgcgt aagcggggcg attgagttga atcgaaagca gcgaagcggg 121 tgaaacttaa tccaactccc aaaccctgac agtaacgttt tgaatagcta gtacatttgc 181 gtcaatccaa aaacgtttag gattatccgt cgcgatttca aaaaacatca tcggtttaac 241 agtcatagac tcatcaccat tcttaacaac aatgtttagc gtcttatttg cgtttgttac 301 tcgtataaca tgagtttcgc gattagccat gttcatttgt ctccgtgact ctggaaagtt 361 aaggctcatt atatcacctc acaatgtcca tttcaattaa atgagtttcg cataatttgt 421 atttgataat taccaaatct tcgaaatcaa taatttctgt gagcttggtt tcgccttccg 481 taatttctaa atgccgatta agagccacgt ttagaattat gattgaggta gatgttaggt 541 tttttattct cgctaacatg ggtcaaattt ccttcttctt cgttccaaaa taattgaatc 601 gctttctcat gctggttact ttgagaatct aaacgataat gacctcccca aaccacgtct 661 aaataggata ttatcgtctc atctaagtca gccgtccagt tagtgacgag gtatagagcg 721 ccgtaatgcg ttgagcgtac atatctagac attattagaa ctccaagtaa aatgagacat 781 tagcgacgta agcgttccaa atactaaatc ttgcgccaac ggattcaaat acaccgacat 841 caacaaaagg agcgacaata ggagcgacac cgcccgcatt atatttagct ttttcgtaac 901 ctgtcgcaat tcccgtttgt acgcccgctg accaatgaac attgctgagc aactcgtcac 961 gcttccaagt ataaacgact gcaagcgagt tagacctttc agagaaggaa ttggtaaagc 1021 ttgtggtgtt gattccccac tgtggggtga tttggtattc aagtccataa gttttgtgaa 1081 cctcgttata atcaaaagaa cggttaaagt ggaatgaata ggcgtctccc atgaacgcct 1141 tgaatttatt ttcgctagcg tcggcgggcg aaatcatgca gacagcgtga accaaaacaa 1201 gtgcgactcc gtagctcccc gctttagcgc agatactggc gcaagttaaa atcgcgatag 1261 cgacgataac gtaaatataa aagtcagaca ttatgatttc tccaaataaa agggcgtatc 1321 ggatttgccg ccgatacgcc ctaatgtcaa attatttaag tttccagttg attacttgaa 1381 tttcagattg tggtaacaac gaaacaaggc tctcaagctt ggctttattt tcgcctttga 1441 ggatgataat ttcaccaccc tcaagagtta ctttgcatgt aaccaaagtt atgcaacttc 1501 gatttcgtaa attgtgtgct caggaaaacg cttgccctga actttacgag tcacaacttc 1561 gtatgcctta ccggattgga acttagccgc taaacctttg tgaaggtaga tgtcgaattg 1621 ttcgccgctt tctagctctg taaccgttga tttcatcata tccggttttg cgtcttcttt 1681 agcgcttacg tgctcagcga actctttttc aattttgatg aaatgagact tcgccgcgcc 1741 gtctttgcca taatcaagag aaagaagagg aagagataca gccgctacac gtttgaaagt 1801 tggttgagtt gatttagaca taatttgctc ctagtccgta gactttaaat taaaaggctt 1861 aacgccgttt ggggcttatc ccctgaaaga gattacatag tatagaatct gtttctcgat 1921 gtaaactact ttttcacgtt tttctgattt ttctttcaat gaagcgaacg ccacgcgaaa 1981 ccgaaaaagt cggcgcttcg cttttataaa gaacttcgcc gccgctcgca acttcgatta 2041 attgctcgtg agtgagtcta gcgccctttg acgcggtttt tgtgctcccg tcttcgcatc 2101 gcatagtata aagctttttc cctgctatag cgcaaaaggt tgcttttgcc tctatatccc 2161 acgcgcccaa ttttgtcgcg tctatttcca aatctcccaa cccctcgcaa atgattgagt 2221 cagtatcaca gtaatatgga ttcttaacgc tgcataatgc ttgcatcata aacgctcgta 2281 catatcccgt tatgctcgcg gcggtcgcaa cgttaaggaa tttgagttca tcatcgtaaa 2341 ggtcacgctc atataatagg ttgtcgccga actctgtagc gcattcatag tcaggattgt 2401 tatattcgaa ctcttccgcc gtgttaatgt tttgtactga atactgttta tacttcgtgg 2461 ggttcgtcgc gtacttgcca tacaaactat tcataaaaag cttcgcgaat aatctcttcg 2521 cagcatcgcc gtttatggtc gcttcctctt tcatcacgta aaagtggtca acgtagtcgc 2581 caaagcatat ttcatcttcg aaggtcaaca cctccaaaat ttcgacctcg cgcaaaagtc 2641 ccaaatcttc cgccatgtaa tattcatgtc cagttatatg aaactcataa acctgtttat 2701 cgtctggaaa agagagcgct cctttttcgg ttctaatagg aaagtgaccg ttagattggc 2761 acttcaaacg aataaaaatt ttagggtcgc gttcttccgg taagcgtcgt gacttcgctc 2821 ttttattgcc atacgcgtgc ggatatttca tagcgtaagg atacgcggaa ttaatatcca 2881 cataataaaa atctttttcg aatacacccg ttttaaatgc ctgaactctt ccaccaaaat 2941 aaaagcggtt tagattctcg aaataattaa tattcgttct aggcgtttta agacccgcca 3001 tattattaaa aacttttagg gcggtactcg ctagcgtgat attgaatccg taatcagtca 3061 caaaacgagt aaccaactca tgcaaataaa cgcagtcgct tttcaagtaa tcgcgtatcg 3121 ctttccagtt ttccggttta tatctctctt ccttttcaaa taggtcatac gatatttcat 3181 ccttttgata agcagctagc ggaataggta aaatattata agaatcgcga aaggtgcatt 3241 tacctatttt aaattcagcg atacgacccg cgataatttt aatatctggt tttatctcgc 3301 attcccacaa aacaaaatgc cagtcaaagc gtccgccgtt atgagcgtaa atcgtccact 3361 cttcgtccct cacgaaatta acgaaatcaa tagttttctc gaactcataa tattgttcat 3421 taatatatag accccataaa aacggctttg gaactctgcc gtatttaaac gggtcggtct 3481 cacaatcagc cgcgcctatg ctttctaaat cgtttcgctt cttacccatt ttaactacct 3541 ataaaatgcg gactgaaaac ctgtaataaa agtatctgcg ttttcataac gttcaaccca 3601 atccgccatt tcttccgcca atatctcagc ttcaaaactt tcagttgaaa gataatcacc 3661 agtttttacc caaagctttt gacgcggcgg tacttggtga atctccaata attcggttac 3721 ataattaaac gtattattta agaatgaaaa ttggtcaacg attggaataa taaattgttt 3781 cattttagaa cgtttattaa atgttttaat tactccgttt ttaactttta tattaggcgc 3841 ttgtttaccg ttgtacggta caaacgccac cttccaacgg ggcaacgtct ttaatgtttg 3901 atgataagca ttttgaacta ttttcaaatt atctttattt gagctacgaa atacttttat 3961 attttgtccc tgatatatat ctttaaattg ttttccgtaa atagtaatta accttttatc 4021 tgccggactt agctttttgc gtaaatcaat gttaaaatta acaaatggtc ttagcgcctt 4081 aaataactct ttgtattctt tttctttcat aggagctcct taaatggata ctagcgataa 4141 tcatcaaatt agcgatttag tgagcgctgt aagcgattca acaatcgaag ataacaccac 4201 tttaacagaa aacgagggtt cagtcaattt ccaaagaatt gcagaaatcg gcgaagtttc 4261 agaaaatcca gaaaccccgc aaagcggcga aatcccacgc gagctaatgt ttggcgagaa 4321 cggggaaatt cgtacagacg cagacggcgc gcaattcgac cctggcaagc atgtagtcga 4381 cgagcaggga cacccaaagc tgaccgccaa gggcaaactg cgtaaaaagc gtgggcgcaa 4441 gtcagggagc ctcgcgacca cgaccgcagc ccaaaccgct caaaaggtag cagtaagcga 4501 aaaagaaaaa tatcgtgtcg taggcgcggg agcagcgaac gccctaatca gcgttgggat 4561 gatgttgggc ggggaagatt tcatacccat tcagaaccaa cacattgacg agaaaaagaa 4621 cttagaagcg gctttctctc aatgggcgga aactcaggag gtcgaggata tcccgccaaa 4681 cctagcatta acaattgtga tgggcgctta ccttctcccg cgtctctcaa tgccaaaaca 4741 ggttagtaaa ttacggctag ctaaagagtg gtttaaaatg aaattcttta gaaagccgaa 4801 agcggcgaaa gtcgcgaagt cagccttcga cccaaacaaa gaaaataagg agggctaaca 4861 atgccacatt gcgcgatagt tggaatgacc gaaagcggca aatctagttt gggtcgaacg 4921 ttcgctaagg aattacaaaa gagcggtatt caaataatag tattcgaccc gcttggcgac 4981 cccgaatgga ataacgcgct agatttagaa aacgcgttta taaccgataa cgaaaatgaa 5041 tttttaaatg tttattggaa ctctaaaaat tgcgccgtat tcttcgatga ggcgggagac 5101 tacgcgacca atcataataa gggaatgatt agaacagcca ccaaagggcg acattggggt 5161 cattctaatt tttatatagc gcaacgtggt aatttattag cgcgaactat acgcgaccaa 5221 tgcgctaatt tgtatatgtt tacctcatcc aaagatgatg ctaaaatata ttctacagaa 5281 tttaatgatg ctgaattatt aaatgtacct gatttaccgc aaggtactta ttatacttgc 5341 ggtagattta aaaaagccgc aaggcatatt ttatttaaag ggaagtcaaa atgattccaa 5401 ttgctttacg atttgttgca tatttgggag cggctttatt tttagagtca caattaaata 5461 aagagtcaac tggagaaaag aaagatgttg ataaagctaa aacgccgaaa gctaaagcta 5521 cagatggaaa tcgacaagaa agcggaagcg aagaaacaag cggaagcgga agtgaagaaa 5581 caagcggaag cggaagcgaa gaaacaagcg gaagcggaag cgaagaaaca agcggaagcg 5641 gaagctaaga aacatgcgga agcggaagcg gaagtgaaag caaatagcgg ttcggataac 5701 ttggagtaat ttaatatgct tctagattct caagctgcaa aagtcgccct ttgggcgggg 5761 ctttcagctt atttgttcga ggcgttcata cgccccaaaa taggaaagta attatgtcat 5821 taaaagacat tttaataatt tcaggcgttt cagctatgac aacgctcgtt attaacaatt 5881 cacccctaaa gcgttatttc cgttaatcgg tcttaacagt acttagctaa attggagtat 5941 ctcacgatgc ctaaaaaata tatgccttac gcgattaatg cagtggtagc gggcgtaacc 6001 gcttacctta tcacttccgc agtcgcaaaa cagcgtcaag ttcaatggta caatccgacg 6061 acttggttct aattctacta gtcaaactcg ttcactcaat tcataaattt cggagaaagc 6121 aaaatggctt ttcgttctaa taaaatctta cctaacttca acggcgtagg cgcgggtcaa 6181 actgctacgc ttgacgttcc tatcggctca acttaccacc aagtagcgtt tgaaattgcg 6241 ggcgttacac ccgctcagtt gactaacttc aaagtgcttc taaacggtat cccacacatc 6301 gaacttccga gcgttgaaat ccttaacact cttaacaagt ttgacggtcg cctaaactca 6361 gcaggtgttt ttgttctaga cttcgaacgc ttcggtcttc ttacgcgtca agctcgcgag 6421 ctaaccgcaa tcgggacagg tatgggcgac gctgacccga atcaggtgcg cactttccag 6481 attcaaatgg atatagacgc ggcagcaact agcccgtcta ttaaagcgac cgcgacaacg 6541 tcgcccgctt ctatgtctgg attggttcgc aagattcgtt tgttcaaccg taaccctacg 6601 ggcgcgggct tgttcgaagt ttccgacctc ccgaaaggag acatgattaa caaagtgttt 6661 ttccacgctg ccgcctcatc tgacattgac gcgctttcag ttgaaattga caactatgtc 6721 aagttcgagc gtagcgcggc gctaaactca gacatccaac aaagtgacgg cgttcgcatg 6781 cccgctccga cgatgttcgt gtttgaccct actgaactag gttacggtgc ggaagtggtc 6841 gacactgttt atccggaggg cacgccgcga gcgggtcaga acgtttcgga tttacgcttc 6901 aagttgcgca tggcgggcgc gcaagcgcaa cttttaattg ttgagtatct tggtgcggtt 6961 gaacgctaat ttctcgacac ttacactgta acttttaaaa ccaatgggcg ggctagatgc 7021 tcgccctttt tattaggaat attaaaatga atgtagattc aaacatgttt gaaaactggt 7081 tcaccacaac tacgcctcaa gggcaacaag tgagtctttg ggataatatc cttaatgcgg 7141 gcggccaagt gattaacagc ttacccgacg tagttgacgg aatcattgat actaagcttg 7201 atgccgagcg aaaaacccaa acggagaaga ccgagaatat cgcctcgact aatccaaacg 7261 tcgcaggtga agcgaactcc aagaatccaa tgaacaacca aaacatgatg atgggcatgt 7321 atgttgttgg cggtctattg gctactgtgg tactcatcaa agttctaaag taaggagtcg 7381 gcttatgcct tttttacttc caatagccgt tttgggcggt actggtattt ttgcttggaa 7441 agcaggcgac gcggcaggta acgcagttga gacagcagca aaagttgcag cggtcggcgg 7501 cgttttatac ttaatctaca aaagtaaggg ttaaattatg caagcagcgg gcggcggcgg 7561 aatgtcagca gggtcaagcg cgggcgcggt gggcggagct tcgggctcta gtatcggcgg 7621 cgtaaatatt gacttgagcg gtaaaggtga catgaacgtc acttatctgg tagcgggcgg 7681 gttagcactt ggcgcgctat ttattataat gagctacaaa taaggcgaaa ccatgcaaaa 7741 atttgaacct gtttcaatcg acgaagcact ggcaaacatg ccaaagagta ttcaccgcca 7801 cactgattta gtaaaagact taatccgcaa gggcgtacaa catgcctata aattcggtga 7861 gctttatgta atctttgagc cgtgggaaaa cactcttatg gtgcaatgtg tcgaggggag 7921 cggtttaaat gatgcgttcg tcaaagccct ttatgattgc gcgacaaaca acgggtttga 7981 atacattcag tttgtcacac cgcataaggg gcttcataaa atggtgaaat ctctcaaccc 8041 cgaaaggata gagtatcgct atatttgtaa actagatgag gtgaaactat gagtttttct 8101 cgcggcggct caagtaagtc cgccaccaca aacgaacaaa acccaattac agcgaccgat 8161 aatgctatgg tcgcctcaga gggcggaact atcacagtga cggacggcgg cgcgttcggt 8221 ctagtcggcg acgcggtcga cgccgtgaca aagacgttct ctgatatgat tttcagtaac 8281 gaaaaagtga ttgggagctc gattgacgca gtaactgctg caacgtctga cgctattttt 8341 agcaatgaaa aagttgtagg cgacgccatg ttgacgaacg agtcaatcgc caatagcgca 8401 atagacgcgg gacttgagta ccaaaaaggc tcaaacaacg ttatcaccga tacactcgaa 8461 agagtgtttg acttcgtcgg agacaccgta accaagagtc aagacactat tagttcaagc 8521 gctacagcgc tctttgactc cgccaagtca gacgagctca acaaatcgca agacatgcaa 8581 caaacgattc gaacgctcgc tatgtatgcc gcggtcggtt ctgttgcttt tatcaccatc 8641 aaatacgctt ttaaataggg gctttataat ggctttaatt cgtgactatc atctaaccga 8701 agtcgcaccg ctcgaagctg aaacgcgagt ggtcaacgtt gtcggtaaca ctatcgtagt 8761 tcgtcgagca gtggacgcgg gcgcggttcg cgtaaaagcg tacacaaaag acggcggcgc 8821 gctcgttact gataacgttc tgtccgcagg tgaaaagatt cgaaccgctg taaaatttac 8881 acgtctcgaa atcactaacc tcgaatcaac taaaaaacat atccacatca cagcaggtga 8941 gggcgacttt caatccgacg tcgttgaggg agaagtaaca gttctaacga gcgacagtag 9001 cccgctaaaa gtggttgggc aagttgtgac tactccaagc aactcaacgc ccaccgacat 9061 agtcgggact gtaataacgc gtgaggcgaa atttgacaac gtgtcgcgca attacgcaca 9121 cactagcggt actcccacat ccattccagt tcccgcgaac gctgtcaagt ttgggattca 9181 aaatgtaaca gcgggtgagg gcgcagtata tttgagcggt gcacacgttt acattcaacc 9241 caaacaagtt ttttcgattg agtgctcaga cgatttagtg gtaacgctcg accaaagttc 9301 gcatagcggc acatataacg ttttctgggt aactcagcca taagggggac ttatggaaat 9361 caaccctctt tttattgtaa tcccgattat aggaatttac gtcatgagca aacaagttcc 9421 atcaattcac ccgtcgcggg gctatcgcaa caataatccg cttaacattc gaattagttc 9481 gagtaaatgg aaaggggaaa ttcctcattc caaaaacaca gacggggctt ttgaacaatt 9541 ttacgagcgc cgttttggat accgcgccgc agccaaattg attttaaact acaaccgact 9601 ttataacatt ttcacagtcg agggtattat aaaccgatgg gctcccgcag tgggcgaaac 9661 tccacaagga caatattata ctaaccatac tcaacaatat attgattatg tttgtaagat 9721 tactggattc gagcccaaca cattaatcaa cggctcaaac gtcgggaagt tggtttgggc 9781 tatggctcgt ttcgaaaata cgccgccaca tacagacacg ttagagcatg taaacgacgc 9841 ggtttctgaa tttgctcaca ttaactacta ggagatattg agtatgtttt tatcggcacc 9901 gttaccagtt ccacaagagt ttattactcg cactaaagtg ttgggcgctc caagcgtacc 9961 caacgcacaa gagataggag cgcccgcgcc taaaacatgg gttcagatga gccaaattat 10021 tcccgcgaca tgcgagcgac ttttaattac tgtaagtcgc gcctatagct cagcgagcgc 10081 tgaaacgggg tttattatca atagagcgag cgacgggcta aggatgtatg atgtcaaccc 10141 aacatcaaag ccaaatactc acgataccaa cacacatgaa attcagcgcc ctgccgaggg 10201 gtggggcgct cttaaaatat acatcaatac gagcggcgcg ggcacaatca gatttgtgat 10261 aattcaccaa ctcgctaact tatattcata ggtgataaaa tggataaaaa gaaagtaaca 10321 aatattattc tcgcaatggt cgcggcaggc gttacagcgg cgggcgcgac gaatatcaat 10381 ttcacggtca cacctgcaaa cgatgagcaa aaattacttg agtgcttaac cgtcattgac 10441 gggatgatta aaactcacag taacgactta gttaaagtaa ctgaaatcct catacaaggc 10501 tcaaaacctt aaaaccccga ggctgtccaa cgtgctcccg ttgttcagcc tctttttcta 10561 ccgttcatac attctaacgt ttgtcaaatt taaatgtcta aagtgtgacc cacgagcgaa 10621 aaggcgcgcc acacac //