LOCUS MG592469 10436 bp DNA linear VRL 24-JAN-2018 DEFINITION Vibrio phage 1.095.O._10N.286.46.E10, partial genome. ACCESSION MG592469 VERSION MG592469.1 DBLINK BioProject: PRJNA328102 KEYWORDS . SOURCE Vibrio phage 1.095.O._10N.286.46.E10 ORGANISM Vibrio phage 1.095.O._10N.286.46.E10 Viruses; dsDNA viruses, no RNA stage; unclassified dsDNA viruses. REFERENCE 1 (bases 1 to 10436) AUTHORS Kauffman,K.M., Hussain,F.A., Yang,J., Arevalo,P., Brown,J.M., Chang,W.K., VanInsberghe,D., Elsherbini,J., Cutler,M.B., Kelly,L. and Polz,M.F. TITLE A major lineage of nontailed dsDNA viruses as unrecognized killers of marine bacteria JOURNAL Unpublished REFERENCE 2 (bases 1 to 10436) AUTHORS Kauffman,K.M., Hussain,F.A., Yang,J., Arevalo,P., Brown,J.M., Chang,W.K., VanInsberghe,D., Elsherbini,J., Cutler,M.B., Kelly,L. and Polz,M.F. TITLE Direct Submission JOURNAL Submitted (29-NOV-2017) Department of Civil and Environmental Engineering, Massachusetts Institute of Technology, 15 Vassar Street, Bldg. 48-417, Cambridge, MA 02139, USA FEATURES Location/Qualifiers source 1..10436 /organism="Vibrio phage 1.095.O._10N.286.46.E10" /mol_type="genomic DNA" /isolation_source="iron chloride precipitation of 0.2um-filtered seawater, resuspended in oxalate solution" /host="Vibrio sp. F12" /db_xref="taxon:1881401" /lab_host="10N.286.46.E10" /country="USA: Massachusetts, Nahant, Canoe Cove" /lat_lon="42.419 N 70.906 W" /collection_date="2010-10-13" /collected_by="Kathryn Kauffman" gene complement(119..376) /locus_tag="NVP1095O_01" CDS complement(119..376) /locus_tag="NVP1095O_01" /note="NVP1095O_01" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AUR87125.1" /translation="MCIQFPHSRDQMNMATKTNDPTHVIRVSNFNKRVFVYLCRGEYK MPIHPHSYFELTTDTPDKYWIDANVIALDKVSLRIIELSND" gene complement(376..585) /locus_tag="NVP1095O_02" CDS complement(376..585) /locus_tag="NVP1095O_02" /note="NVP1095O_02" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AUR87126.1" /translation="MARYVKSKLTNVLYLITNWMDEDEDDIIEYMHDQWGDHYELDPE PLPYESGVKLYWCQIRRSLIHCKGA" gene complement(588..1202) /locus_tag="NVP1095O_03" CDS complement(588..1202) /locus_tag="NVP1095O_03" /note="NVP1095O_03" /codon_start=1 /transl_table=11 /product="TMhelix containing protein" /protein_id="AUR87127.1" /translation="MSDFYIYILVAAWVLICASMAAKQGLYGIALVLVLAVCVISPAD ADEVEPLELEDTWLDFSDFGMPTLLEPLEGKTKIFMGEGYSKHFDQSYDYNEVHENYG FEYKITEKWGVNFTTFKNSYSERSNALAVVYTWKHYSIVDNVRLSAGLQAGIADGYEK AKYNAGGFAPIVAPFVDIVVYESIGARFSIWNAYVANVSFYLEF" gene complement(1214..1369) /locus_tag="NVP1095O_04" CDS complement(1214..1369) /locus_tag="NVP1095O_04" /note="NVP1095O_04" /codon_start=1 /transl_table=11 /product="TMhelix containing protein" /protein_id="AUR87128.1" /translation="MAYIMANLIFLASSLCIVVVARNTKTGVVDIAFILMFHLLGSVG IAALFAN" gene complement(1566..1898) /locus_tag="NVP1095O_05" CDS complement(1566..1898) /locus_tag="NVP1095O_05" /note="NVP1095O_05" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AUR87129.1" /translation="MTKATQPTFKRVAAVSLPLLSLDWGKDGAAKSHFIKIEKAFVDH VSAKEDAKPDMMKSTVTELESGEQFDIYLHKGLAAKFDAGKAYEVVTRKVQGKRFPEH TIYEIEVA" gene complement(2013..3608) /locus_tag="NVP1095O_06" CDS complement(2013..3608) /locus_tag="NVP1095O_06" /note="NVP1095O_06; aclame_best_match:protein:vir:2124; tara_best_match:OM-RGC.v1.001450554; cvp_best_match:RF_PEP_9626351; pfam:PF03175|pog:POG0334" /codon_start=1 /transl_table=11 /product="DNA polymerase type B" /protein_id="AUR87130.1" /translation="MGKKRNDLESIGAADCETDPFKYGRVPKPFLWGLYINEQYYEFE KTIDFVNFVRDEEWTIYAHNGGRFDWHFVLWECEIKPDIKIIAGRIAEFKIGKCTFRD SYNILPIPLAAYQKDEISYDLFEKEERYKPENWKAIRDYLKSDCVYLHELVTRFVTDY GFNITLASTALKVFNNMAGLKTPRTNINYFENLNRFYFGGRVQAFKTGVFEKDFYYVD INSAYPYAMKYPHAYGNKRAKSRHLPDERDPKIFVRLNCRSNGHFPIRTEKGALSFPD NGNVYEFHITGHEYYMAEDLELLHEVEILEVLTFEDEICFGDYVDHFYVMKEEATINK DTAKRLFAKLFMNSLYGKYATNPTKYKQYSVQNINTAEEFEYNNPDYECSTEFGDNLL YERDLYDDELKFLNVATAASITGYVRAFMMQALCSVKNPYYCDTDSIICEGLGDLEID ATKLGAWDIEAKATFCAIAGKKLYTMLCEDGSTKTASKGARLTHEQLIEVASGGEVLY KSEAPTFSVSRGVRFIERKIRKT" gene complement(3614..4189) /locus_tag="NVP1095O_07" CDS complement(3614..4189) /locus_tag="NVP1095O_07" /note="NVP1095O_07; Putative terminal protein" /codon_start=1 /transl_table=11 /product="putative terminal protein" /protein_id="AUR87131.1" /translation="MKDKEYKELFKALRPFVNFNIDLRKKLSSSDKRLITIYGNQFKD IYQGQNIKVFRTSNKNNLKVVQDAYHQTLKTLPKWKVAFVPYNGKQAPSIRVKDGIIK TYNKRSKLKQFIIPIVDQFSFLNDTFNYVSDLLEIHNVPPRQKLWVKTGDYLSTESFE AEILAEEMADWVERYENADTFITGFQSAFYR" gene 4203..4919 /locus_tag="NVP1095O_08" CDS 4203..4919 /locus_tag="NVP1095O_08" /note="NVP1095O_08" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AUR87132.1" /translation="MKTDDNQQLADLVDAVADSTIDDNTTLSENDESVNFQRIAEVTD IPESAETPEMPRELLFDENGEVRTDADGNTFDDSKHVVDENGHPKLTGKGKLRKKRGR KSGSISTTNAAQTAQKMAVSEKEKYRVVGAGAANALISLGIMLGGEDFAPIQNQHIDE KQNLESAFSDWAQTQEVDDIPPNLALTIVLGAYLLPRLTMPKQVSKLKLIKEWFKLKF FKKPKISKQTFDPNKESKED" gene 4922..5455 /locus_tag="NVP1095O_09" CDS 4922..5455 /locus_tag="NVP1095O_09" /note="NVP1095O_09; tara_best_match:OM-RGC.v1.021972741; InterPro:IPR027417; Putative packaging ATPase" /codon_start=1 /transl_table=11 /product="P-loop containing nucleoside triphosphate hydrolase" /protein_id="AUR87133.1" /translation="MPHCAIVGMTESGKSSLARKMAVELQKSGIQIIVFDPLSDPEWS NCLDLNNAFITDNENEFLNVYWNSKNCAVFFDEAGDYATNHNKPMIRTATKGRHWGHS NFYIAQRGNLLARTIRDQCANLYMFTSSKDDAKIYSTEFNDSELLNVPDLPQGTYYTC GRFKKAEKHILFKGKSK" gene 5452..5679 /locus_tag="NVP1095O_10" CDS 5452..5679 /locus_tag="NVP1095O_10" /note="NVP1095O_10" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AUR87134.1" /translation="MISLPLRFIGYLVTALLVETTISKTVIKKESLPDKEKKPNDSNE NKKPSNDGSDEKLAEVEDKGNGASDDESDSE" gene 5710..5805 /locus_tag="NVP1095O_11" CDS 5710..5805 /locus_tag="NVP1095O_11" /note="NVP1095O_11" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AUR87135.1" /translation="MLINPKAAKLALYVAISTYLFEAFIRPKIKG" gene 5916..6041 /locus_tag="NVP1095O_12" CDS 5916..6041 /locus_tag="NVP1095O_12" /note="NVP1095O_12" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AUR87136.1" /translation="MPKKYMPYLINAAVAGATAYLVTKATADNKTVVWYNPASWF" gene 6097..6951 /locus_tag="NVP1095O_13" CDS 6097..6951 /locus_tag="NVP1095O_13" /note="NVP1095O_13; tara_best_match:OM-RGC.v1.009973837; Double jelly roll capsid protein" /codon_start=1 /transl_table=11 /product="double jelly roll capsid protein" /protein_id="AUR87137.1" /translation="MAFRSNKVLPNFNGVGAGQTATLDVPIGSTYHQIAFEYSGMTTA QLTNFKVLLNGIPHIEIPSVEILEAISAFNGRGQTAGIFQLDFERFGLLTRQARELTA IGTGLGNTDPNQVRTFQIQVDIAAGAISPSLKATATTSPASFAGLVKKIRLFNRNPTG AGLFEISDLPKGDIINKVYFQAGAASSQINKLTLEIDNFVKFERSNALNDAIQKDDGI RVPQTKYSVFDPTELGYGAETVDTRYPMVEGNPRSGQNVSDMRFKLDMAAADNMLLLV EYLGTVER" gene 7021..7347 /locus_tag="NVP1095O_14" CDS 7021..7347 /locus_tag="NVP1095O_14" /note="NVP1095O_14" /codon_start=1 /transl_table=11 /product="TMhelix containing protein" /protein_id="AUR87138.1" /translation="MNIDSNPFENFFATTTPNQQQTGIWDSIIGAGSEIIGSLPDVVD GVIGNALNGKEQEKVDSNQNITSSDPSVSGDANKPSVMADNTGKYILYGFGGLITLVV VAKLIK" gene 7357..7509 /locus_tag="NVP1095O_15" CDS 7357..7509 /locus_tag="NVP1095O_15" /note="NVP1095O_15" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AUR87139.1" /translation="MPFLLPLAVLGGTGIFAWKAGEEVGSQTSNLLKIGAVGGSLYLL YKLQKG" gene 7513..7704 /locus_tag="NVP1095O_16" CDS 7513..7704 /locus_tag="NVP1095O_16" /note="NVP1095O_16" /codon_start=1 /transl_table=11 /product="TMhelix containing protein" /protein_id="AUR87140.1" /translation="MQAAGGGGMSASSAAGGAGDASGSSIGGVSIDLSGRGSEMNLTY IIGGAVVLGLAFIFMGKKK" gene 7706..8065 /locus_tag="NVP1095O_17" CDS 7706..8065 /locus_tag="NVP1095O_17" /note="NVP1095O_17" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AUR87141.1" /translation="MNKFELVSVEEVLAKMPKSIHQHVELVKEQMRENIQHGYKFNDL YVLFEPWDKTLMVQCVEGKGLNSEFVEALYKAAINNGFEYIQFVTPHKGLHRMVKELK PERVEYRYICKLSEVNL" gene 8062..8532 /locus_tag="NVP1095O_18" CDS 8062..8532 /locus_tag="NVP1095O_18" /note="NVP1095O_18" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AUR87142.1" /translation="MSFSKGGSSKSATTNEQNPITATDSAIVAAEGSTVNITDGGAFD VVRDIIGSNESVVNDVILSNERISDTSINAGLDYFGQANASIQDSIDKVFDFANETVS QSQDTIASSAQAIFDSAKSDELNKSQDLNATIRELSKWAVFGVVGYTALRYAFK" gene 8544..9149 /locus_tag="NVP1095O_19" CDS 8544..9149 /locus_tag="NVP1095O_19" /note="NVP1095O_19" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AUR87143.1" /translation="MPIIREYVLNDLLPNQGNSKVINVTGRTIVIRRATNAGRVRVKA YTKEGGSLIADNIMDAGEKITTATEFTRLEVFNFEVDIRNVAITAGDGDFQSDVVEGE VTVKTDSSNPLVIASPKYVTSSSFIQHVSGTNSPLIPPAGTVEFGVQLQGADGAVVIE GGAIFLTKNQLYSSKFGDSSTAFIPDWAVSATYVVQWVAQD" gene 9159..9677 /locus_tag="NVP1095O_20" CDS 9159..9677 /locus_tag="NVP1095O_20" /note="NVP1095O_20" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AUR87144.1" /translation="MNLNPLFIIVPILGLYIMSRNSPSLHPSRGYRNNNPLNIRVSNS TWKGEVPANLNTDGSFEQFYDRANGYRAAAKLILNYNKLYNIFTVERIIARWAPAKGQ APDGQYYTNHTQNYIDYVVKNTSLDANSLINGSNVGELVWAMARFENLPPHTDSLEYV NESVSEFKDIDY" gene 9688..10077 /locus_tag="NVP1095O_21" CDS 9688..10077 /locus_tag="NVP1095O_21" /note="NVP1095O_21" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AUR87145.1" /translation="MFLSAPRINPLKVATPLAVGGIAQLPNPAPKQWLRMDTIIPAHF GCIVITVGRHYSSAPAENGFFIERVSDGYRLYEINPFAAANTYGEASYTIQRPLEGWA GYNFLSNTSGAGTINITAVPQALDLYS" gene 10087..10299 /locus_tag="NVP1095O_22" CDS 10087..10299 /locus_tag="NVP1095O_22" /note="NVP1095O_22" /codon_start=1 /transl_table=11 /product="TMhelix containing protein" /protein_id="AUR87146.1" /translation="MTNANTQGKKFNVIAALIAALSVGFGSSQMTFNYSPPDEMQMIE CITVIDKLVTTHNGDIKAVVQMLTAD" ORIGIN 1 cttttcgctc gtacgtcaca ctttagacat ttaaatttga caacttatgg aatatatgaa 61 acgaaaaaag acccgcgtta gcgggtcgat tgagttgaat ggaatgcagc gaagcgggtt 121 aatcgtttga aagctctata atccttaaac taactttatc taatgcaata acatttgcgt 181 ctatccaata tttatctgga gtgtccgtcg taagctcaaa atatgaatga gggtgtatcg 241 gcattttata ttcgcctcga cataaataca caaacactct tttattaaaa ttcgaaactc 301 ttataacgtg agttgggtca ttagttttag tagccatatt catttggtct cttgaatggg 361 ggaattgaat gcacattacg cgcctttgca atgaattaat gaacgtcgaa tctgacacca 421 ataaagttta acaccgctct cataaggcaa tggttcgggg tcaagctcgt aatggtcgcc 481 ccattggtca tgcatatatt cgataatatc gtcctcgtcc tcgtccatcc agttagtgat 541 aagatataaa acgtttgtga gttttgattt aacgtatcta gccattatta gaactccaag 601 taaaaagaga cgttagccac gtaagcattc cagatgctaa atcttgcgcc gatggattca 661 tatacaacga tatcaacaaa aggagcaaca ataggagcga aaccgcccgc attatattta 721 gctttttcgt aaccgtcagc aattcccgct tgtagccccg ctgataaacg aacattgtcg 781 acaatgctgt aatgcttcca agtgtaaacg actgcaagcg cgttagacct ttcagagtag 841 gagtttttaa aagtggtgaa gttgacgccc catttttctg tgattttgta ttcaaatcca 901 taattctcgt gtacctcgtt gtagtcatat gattggtcga agtgcttcga gtacccttcg 961 cccataaaaa ttttggtctt accctccaaa ggctccaaaa gtgtaggcat cccaaagtca 1021 ctaaagtcta gccaagtatc ctcaagctct agcggctcga cttcatcagc gtcagcggga 1081 gaaataacgc agacagctaa aaccaaaacg agcgcgatac catacaaacc ctgtttagcc 1141 gccatagacg cacaaatcaa aacccacgca gcgacgagaa tataaatata aaagtctgac 1201 atttgttttt ctcctagtta gcgaaaagcg cggcgatacc aaccgaaccc aataaatgga 1261 acatcaatat aaaagcgatg tcaacgactc ctgtcttagt attacgagcc acgactacga 1321 tacaaagcga actcgccaaa aagatgaggt ttgccataat atacgccatt tcctttctcc 1381 taataaaaag ggcgtatcgg atttgccgcc gatacgccct aagttctaat tttaaaattt 1441 ccagttgata gcttgaattt ctgattgggg taacaacgaa acaaggctct caagcttggc 1501 tttgtttacg cctttgaggg tgacaacttc accaccctca agagtcactt tacaagtaat 1561 caatgttacg cgacttcgat ttcgtaaatt gtgtgctcag ggaaacgctt accttgaact 1621 ttacgagtca cgacttcgta agctttgccc gcgtcgaact tcgccgctag acctttgtga 1681 agatagatgt cgaactgctc gccgctttct agctcagtta cggtggattt catcatatcc 1741 ggtttagcat cttcttttgc gcttacgtgg tcaacgaacg ccttttcgat tttgataaag 1801 tgagactttg ccgcgccgtc tttgccccaa tcaagagaaa gaagaggaag agaaacagcc 1861 gctacacgtt tgaaagttgg ttgagttgct ttagtcataa tatagctcct agcccgtagg 1921 ctgtaaaatt aaaggcttaa cgccgttttg gggcttatcc cctgaaagag attacatagt 1981 aaagaatctc tttcgtgttg taaactactt tttcacgttt ttctgatttt tctttcaatg 2041 aagcgaactc cacgcgaaac cgaaaacgtt ggtgcttcgc ttttatatag aacttcgccg 2101 ccgctcgcaa cttcgattag ttgctcatgc gtgagccttg cgccctttga ggcggttttc 2161 gtgcttccat cttcgcaaag catcgtataa agctttttac ccgctatagc gcaaaaggtc 2221 gcctttgctt ctatatccca cgcgcctagt ttggttgcgt cgatttctaa atcacccaaa 2281 ccctcgcaga taatcgaatc agtatcgcag taatacggat tcttaacact acataaggct 2341 tgcatcataa acgctcgcac atatccggta atgctcgcag cggtcgccac gttcaaaaac 2401 tttagttcat catcgtaaag gtcacgctcg tataaaaggt tatcgccgaa ctcagtcgag 2461 cattcatagt cgggattgtt atattcgaac tcttccgccg tattgatgtt ttgaactgaa 2521 tactgtttat acttcgtcgg gttcgtcgcg tacttaccgt aaagactatt catgaacagc 2581 ttcgcgaaca atcgtttcgc cgtatcttta tttatggtcg cctcttcttt catcacgtaa 2641 aagtggtcaa cgtagtcacc gaaacaaatc tcatcctcga acgtcaacac ttctaaaatc 2701 tcgacttcgt gtaaaagctc caaatcttcc gccatgtaat attcatgccc agtgatatgg 2761 aactcgtaaa cgttcccgtt gtcagggaaa gagagcgcac ccttttcagt cctaatcggg 2821 aaatgaccat tcgaacgaca attcaaacga acaaaaattt tagggtctcg ctcatctggc 2881 aagtggcgcg acttcgctct tttattcccg taagcgtggg gatatttcat tgcgtaagga 2941 tacgcggaat taatatccac atagtaaaag tccttttcaa aaactcccgt cttaaacgct 3001 tgaactcttc caccaaaata gaagcgattc aaattctcga aatagtttat attggttcta 3061 ggcgttttca atcccgccat attattaaaa acttttaaag ccgtacttgc gagcgtgata 3121 ttaaatccgt agtcagtgac aaaacgagta accaactcat gcaaataaac gcagtcactt 3181 ttcaagtaat cgcgtatcgc tttccagttt tccggtttat atctctcttc cttttcaaac 3241 aggtcatatg atatttcatc cttttgatac gccgcgagcg gaataggtaa aatattatat 3301 gagtctctaa aagtacattt accgatttta aactcagcga tacgccccgc gataatttta 3361 atgtctggtt ttatttcgca ttcccacaaa acaaaatgcc agtcgaaacg tccgccgtta 3421 tgagcgtaaa tagtccactc ttcgtctcga acgaaattaa cgaaatcaat tgttttctcg 3481 aactcataat attgttcatt aatatataga ccccacaaaa acggctttgg aactcgacca 3541 tatttaaacg ggtcggtttc gcaatccgcc gcgcctatac tttctaaatc gtttcgcttc 3601 ttacccattt taattaccta taaaaagcag attggaaacc agtaataaaa gtatctgcgt 3661 tctcgtaacg ctcaacccaa tccgccattt cttccgctaa tatctcagcc tcaaaacttt 3721 cggtagaaag ataatcacca gttttaaccc aaagcttttg tcttggcggt acgttatgaa 3781 tttctaataa gtcgcttaca taattgaacg tatcatttaa gaatgaaaat tggtcaacga 3841 ttggaataat aaattgttta agtttagagc gtttattata tgttttaata atcccgtcct 3901 taactcgaat gctaggagcc tgtttaccgt tatatgggac aaatgcgact ttccacttag 3961 ggagcgtctt taaagtctgg tggtacgcgt cctgtactac ttttaaattg tttttattag 4021 atgttctaaa tacttttata ttttgacctt gatatatatc tttgaattga tttccatata 4081 ttgtaataag tcttttatcg ctagaactta gctttttgcg taaatcaatg ttaaaattaa 4141 caaatgggcg caatgcctta aataattctt tatactcttt atctttcatg ggagcacctt 4201 aaatgaaaac tgacgataat caacaattag ccgatttagt tgatgcggta gcagattcaa 4261 caatcgacga taacactacc ttatcagaaa atgacgaaag tgtcaatttt caaagaatcg 4321 cagaagttac ggatattcca gaatcggcgg aaacccccga aatgccgcga gagctattat 4381 tcgacgaaaa tggcgaagta cgcacagacg ccgacggtaa tacattcgat gattctaaac 4441 atgttgtaga tgaaaacggg caccctaagc tgacaggaaa aggtaagctc cgcaaaaagc 4501 gcgggcgcaa gtcgggctcg atttctacca ctaacgccgc gcaaactgct cagaaaatgg 4561 cggtaagcga aaaggaaaaa tatcgagtag tcggagcggg cgcggcgaat gctttaatca 4621 gcctgggaat tatgttagga ggcgaagact tcgcgccaat tcaaaaccaa catatagacg 4681 aaaagcagaa cctagaatcg gctttttccg attgggcgca aactcaggaa gtggacgata 4741 ttccaccaaa cctagcatta acaatcgtgt taggggctta tttgctaccg cgcttaacaa 4801 tgccaaaaca ggtaagcaaa ttaaaactaa ttaaagaatg gtttaaatta aaattcttta 4861 aaaagccgaa aatttcgaaa caaacttttg acccaaataa agaatcaaag gaggattaat 4921 catgcctcat tgcgctattg taggaatgac agaaagcggc aaatccagtt tagctcgtaa 4981 aatggcggta gaacttcaaa agagcggtat tcaaataatt gtatttgacc cgttaagcga 5041 ccccgaatgg agcaactgtt tagatttaaa taacgctttt attaccgata atgaaaacga 5101 atttttaaat gtatattgga actcgaaaaa ctgcgcggta tttttcgatg aagcgggaga 5161 ctacgcaact aatcataata agcccatgat tagaaccgcc acaaaaggac gtcactgggg 5221 tcattctaat ttttatatag cgcaaagggg taatttatta gcgcgtacta ttcgcgacca 5281 atgcgctaat ttatatatgt ttacctcatc taaggatgat gctaaaatat atagtacaga 5341 atttaatgat agcgaattat taaatgtacc tgatttgcct caaggaacgt actatacttg 5401 tggtagattt aaaaaagctg aaaagcatat tttatttaag gggaagtcaa agtgatttca 5461 ttaccattac gttttatcgg ttatttagtt accgctttat tagttgaaac aactatttca 5521 aaaacggtaa ttaaaaaaga gtcattacct gataaggaga aaaagccaaa tgacagcaat 5581 gaaaataaaa agccgtcgaa tgatggaagc gatgaaaaac tcgcagaagt cgaagacaaa 5641 ggaaacggag caagcgacga cgaaagtgat agcgagtaac gtttataatt ctaactaggg 5701 gtaataaaca tgctaattaa tcctaaagcc gctaaacttg ctttatacgt tgcgattagc 5761 acttatttat ttgaggcgtt tattcgacca aagataaaag gttagctcaa tgagtattac 5821 agactttttt aaaatcgcgg gtatttcgtt gttaacaacg tatgccatta atcaatcgcc 5881 tttcaagcga tactttagtt aaacggagat acaccatgcc aaagaaatat atgccttacc 5941 taatcaacgc ggcggtagcg ggtgcaactg cttaccttgt aaccaaagcg accgctgata 6001 ataaaacggt tgtatggtac aatcccgctt cttggtttta attaacctcg acactttcaa 6061 gcatttaatt catttagtta ctaggagata aaagccatgg cttttcgttc aaataaagtt 6121 ttacctaatt tcaacggcgt cggcgcgggt cagactgcta cgctagacgt gccgattggt 6181 tctacttacc accaaatcgc gtttgagtat tctggtatga ctaccgcgca acttacaaac 6241 tttaaagtcc tactgaatgg tatcccccat attgagattc catcagttga aattcttgaa 6301 gctattagcg cgtttaacgg tcgcggtcaa acagcgggca ttttccaact cgactttgag 6361 cgttttggtt tgcttactcg tcaagctcgc gagcttacag cgattggtac gggcttaggt 6421 aatactgacc ctaatcaagt gcgcacgttc caaattcaag ttgacattgc agcgggcgca 6481 atctcgccga gcctaaaagc taccgctaca acttctccgg catcttttgc gggattggtt 6541 aaaaagattc gtctatttaa ccgaaaccca acgggtgcgg gtctattcga aatttctgat 6601 ttgccgaaag gtgatattat taacaaagtt tactttcaag cgggcgcggc ctcgtcgcaa 6661 atcaacaagc tcacacttga gattgacaac ttcgttaaat tcgaacgttc gaacgctcta 6721 aacgacgcaa tccaaaaaga tgatggtatc cgtgttcctc aaactaaata ctcagtattt 6781 gacccaacgg aactaggtta tggcgcggaa accgtggata cacgctaccc aatggtcgaa 6841 ggcaatccac gaagcgggca aaacgtttcg gatatgcgct ttaagttgga tatggcggcg 6901 gcggataaca tgctcttact cgttgagtat cttggaaccg ttgagcgtta atgacgctgt 6961 aacattttaa atctaaacca tagggcgggc taatgctcgc cctttttgga gaaaaacatt 7021 atgaacatcg attcaaatcc gtttgaaaac tttttcgcta caaccactcc aaaccaacaa 7081 cagactggaa tttgggattc aattatcggc gcaggttctg aaattatcgg ctcattgccg 7141 gatgttgttg acggcgtaat cggtaacgct ctaaacggaa aagagcaaga aaaggttgat 7201 tcaaaccaaa acattacctc aagtgacccg agcgtttcgg gcgatgctaa caaacctagc 7261 gttatggctg acaataccgg aaaatacatt ttgtatggct ttggcggtct catcactttg 7321 gttgtagtag ccaaactaat taagtaagga gttgttatgc ctttcttatt acctttggcg 7381 gtgttgggtg gaactgggat tttcgcttgg aaagcgggtg aggaagtggg ttcacaaacc 7441 tcaaaccttt taaagattgg tgcggttggc ggcagtcttt atcttcttta taaacttcaa 7501 aagggttaaa ttatgcaagc agcgggcggg ggcggaatga gcgcgtcgag tgcggcgggc 7561 ggtgcgggcg atgcgtcggg ctctagtatc ggcggcgtta gtatcgattt gagtgggcgc 7621 gggagtgaaa tgaatctcac gtatattata ggcggggcgg tagtattggg gctcgctttt 7681 attttcatgg ggaaaaagaa atagcatgaa taaatttgaa cttgtttccg ttgaggaagt 7741 attagcaaaa atgccaaaaa gtattcatca gcacgttgaa ctagtaaagg agcaaatgcg 7801 cgaaaacatc caacacggtt ataagttcaa tgatttatat gttctttttg aaccgtggga 7861 caaaacctta atggttcaat gcgtagaagg taaaggttta aattccgaat ttgttgaagc 7921 tttatacaaa gccgctatca ataacggatt tgaatacatt cagttcgtta cgccgcataa 7981 aggcttacac agaatggtta aagagttaaa gcccgagcgt gttgaatatc gttatatctg 8041 caaactcagt gaggtgaatc tatgagtttt tcaaagggcg gctcttctaa atccgctacc 8101 actaacgagc aaaacccaat cacggcgaca gattccgcta ttgtcgccgc tgaaggctca 8161 acagttaata ttaccgacgg tggggcgttt gatgttgtac gcgatattat cgggtctaac 8221 gaaagcgtcg ttaacgacgt tattctaagc aacgagcgta tttccgatac ttcgattaac 8281 gcggggctcg attatttcgg acaagccaat gcatcgattc aagatagtat tgacaaggtt 8341 tttgacttcg cgaatgaaac cgtatctcaa tcacaagata ccattgcgag tagcgcgcag 8401 gccatttttg attctgcgaa atcggacgaa cttaacaagt ctcaagactt aaacgcaaca 8461 atccgagaac tttcaaagtg ggctgttttt ggggtcgtgg gttacactgc gctacgttac 8521 gcatttaaat aggagtttgt aaaatgccaa ttattcgaga atatgtttta aatgacttgt 8581 taccaaatca gggtaattca aaagttatca atgttacagg ccgcacaatt gtgattcgtc 8641 gcgctaccaa cgcggggcgt gttcgcgtga aagcttacac taaagagggc ggttcgctta 8701 tagctgacaa cattatggat gcgggagaaa agattacaac cgcgaccgaa tttacacgac 8761 tagaagtgtt taactttgaa gtcgatattc gaaatgttgc tattacggcg ggcgacggcg 8821 attttcaatc tgatgttgtt gaaggtgaag ttactgtcaa aactgattcc tctaacccgt 8881 tagtaatagc ctccccaaag tacgttacta gctcatcctt tattcaacac gtctccggta 8941 cgaattcacc gttaattccc ccagcgggca cagtggagtt cggtgttcaa ctacagggcg 9001 cagacggagc ggtagtgata gagggtggag ctatattctt aaccaaaaac cagttgtata 9061 gctccaagtt cggcgatagc agtaccgcgt ttatcccaga ctgggctgtg agcgcgacat 9121 acgttgtgca gtgggtagct caggattagg agcgatttat gaatctcaac cctttattta 9181 tcatcgtacc aattttagga ctttatatta tgtctagaaa ttcgccaagt ttacatccct 9241 cgcgtgggta tcgtaacaac aatcctttaa atatccgagt gtctaactca acttggaaag 9301 gggaagtacc cgccaattta aacactgacg ggtcgttcga acaattttac gaccgagcaa 9361 atggatatcg cgccgccgcg aaattgattt taaactataa caagctttat aacattttca 9421 ctgtcgagcg tattattgcg cgatgggctc ccgctaaagg tcaagctcct gacggtcaat 9481 attacacaaa ccacactcaa aattatatag actacgttgt taagaatacg tcgcttgatg 9541 cgaattcgct tattaacgga tcaaatgttg gtgaattagt gtgggctatg gctcgcttcg 9601 aaaacttgcc gccgcatacc gattcgcttg aatacgttaa tgaatctgtt agcgaattta 9661 aagatatcga ctactaggag aaagattatg tttttatcag ctccaagaat caatccatta 9721 aaagtcgcta ccccgttagc agtgggcgga atcgcacaat tacccaatcc cgcacctaag 9781 cagtggttga gaatggacac tattatacct gctcatttcg ggtgtatagt aattacggtt 9841 gggagacatt actcgtctgc gcccgccgaa aatgggtttt ttatagagcg agtttctgat 9901 gggtatcgtc tgtacgaaat taaccctttc gctgccgcta atacatatgg cgaggcgagt 9961 tatacaatcc aaagaccact agagggatgg gcgggctata attttctcag caatacaagc 10021 ggcgcgggca caataaatat cactgccgta ccccaagcgt tagacctata ttcataagga 10081 aatactatga caaacgcaaa tactcaaggt aaaaagttca acgtaatcgc tgctttaatc 10141 gctgcgctca gtgtcgggtt tgggtcgtcc caaatgacgt ttaattattc accgcccgac 10201 gaaatgcaaa tgattgaatg tatcactgta atcgataaac ttgttactac gcataatggg 10261 gatatcaaag cagttgtcca aatgctaacc gctgactaat ctcaaccctt tcgggcgttt 10321 gaattgtgct cctttcattc gcccgctttt ctaaatgtca agcccttttt ctacgtttca 10381 tatattccat aagttgtcaa atttaaatgt ctaaagtgtg acgtacgagc gaaaag //