LOCUS MG592474 10447 bp DNA linear VRL 24-JAN-2018 DEFINITION Vibrio phage 1.102.O._10N.261.45.E3, partial genome. ACCESSION MG592474 VERSION MG592474.1 DBLINK BioProject: PRJNA328102 KEYWORDS . SOURCE Vibrio phage 1.102.O._10N.261.45.E3 ORGANISM Vibrio phage 1.102.O._10N.261.45.E3 Viruses; dsDNA viruses, no RNA stage; unclassified dsDNA viruses. REFERENCE 1 (bases 1 to 10447) AUTHORS Kauffman,K.M., Hussain,F.A., Yang,J., Arevalo,P., Brown,J.M., Chang,W.K., VanInsberghe,D., Elsherbini,J., Cutler,M.B., Kelly,L. and Polz,M.F. TITLE A major lineage of nontailed dsDNA viruses as unrecognized killers of marine bacteria JOURNAL Unpublished REFERENCE 2 (bases 1 to 10447) AUTHORS Kauffman,K.M., Hussain,F.A., Yang,J., Arevalo,P., Brown,J.M., Chang,W.K., VanInsberghe,D., Elsherbini,J., Cutler,M.B., Kelly,L. and Polz,M.F. TITLE Direct Submission JOURNAL Submitted (29-NOV-2017) Department of Civil and Environmental Engineering, Massachusetts Institute of Technology, 15 Vassar Street, Bldg. 48-417, Cambridge, MA 02139, USA FEATURES Location/Qualifiers source 1..10447 /organism="Vibrio phage 1.102.O._10N.261.45.E3" /mol_type="genomic DNA" /isolation_source="iron chloride precipitation of 0.2um-filtered seawater, resuspended in oxalate solution" /host="Vibrio lentus" /db_xref="taxon:1881323" /lab_host="10N.261.45.E3" /country="USA: Massachusetts, Nahant, Canoe Cove" /lat_lon="42.419 N 70.906 W" /collection_date="2010-09-18" /collected_by="Kathryn Kauffman" gene complement(119..343) /locus_tag="NVP1102O_01" CDS complement(119..343) /locus_tag="NVP1102O_01" /note="NVP1102O_01" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AUR87636.1" /translation="MNMATKTNDPTHVIRVSNYNKRVFVYLCRGEYKMPIHPHSYFEL TTDTPDKYWIDANVIALEKVSLKIIELSND" gene complement(375..584) /locus_tag="NVP1102O_02" CDS complement(375..584) /locus_tag="NVP1102O_02" /note="NVP1102O_02" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AUR87637.1" /translation="MARYVKSKLTNVLYLITNWMDESEDDIIEYMHDQWGDHYELDPE PLPYESGVKLYWCQIRRSLIHYKGA" gene complement(587..1204) /locus_tag="NVP1102O_03" CDS complement(587..1204) /locus_tag="NVP1102O_03" /note="NVP1102O_03" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AUR87638.1" /translation="MIMTIYFCLGLCILYAAHRLAAAGYHGVALSFVLVLCIASPVGA SETEPESLDTSGTWLDFSEFGLPTVLEPLEGKTKIFMGDAYSYHFDRSYDYNEVHRTY GFEYKITENYAIAFTNFTNSYNVQSNALSIVYTWRDYRFDNDIGLAVGAQGGIADGYE KAKYNSNGFAPIVAPFVDITVYESIGARISVWNLYVANIAFYLEF" gene complement(1215..1373) /locus_tag="NVP1102O_04" CDS complement(1215..1373) /locus_tag="NVP1102O_04" /note="NVP1102O_04" /codon_start=1 /transl_table=11 /product="TMhelix containing protein" /protein_id="AUR87639.1" /translation="MFTLSVILVLGLSSICIAMVGNEANRNITDIVFMILFHLVGAAG FAALCVIQ" gene complement(1571..1909) /locus_tag="NVP1102O_05" CDS complement(1571..1909) /locus_tag="NVP1102O_05" /note="NVP1102O_05" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AUR87640.1" /translation="MTKQAKTFPTFKRIAAVSLPLLSLDYGKDGAAKSHFIKIEKAFV DHVSAKEDAKPDMMKSTVTELESGEQFDIYLHKGLAAKFEAGKAYEVVTRKVQGKRFP EHTIYEIEVA" gene complement(2024..3619) /locus_tag="NVP1102O_06" CDS complement(2024..3619) /locus_tag="NVP1102O_06" /note="NVP1102O_06; aclame_best_match:protein:vir:2124; tara_best_match:OM-RGC.v1.001450554; cvp_best_match:RF_PEP_9626351; pfam:PF03175|pog:POG0334" /codon_start=1 /transl_table=11 /product="DNA polymerase type B" /protein_id="AUR87641.1" /translation="MGKKRNDLESIGAADCETDPFKYGRVPKPFLWGLYINEQYYEFE KTIDFVNFVRDEEWTIYAHNGGRFDWHFVLWECEIKPDIKIIAGRIAEFKIGKCTFRD SYNILPIPLAAYQKDEISYDLFEKEERYKPENWKAIRDYLKSDCVYLHELVTRFVTDY GFNITLASTALKVFNNMAGLKTPRTNINYFENLNRFYFGGRVQAFKTGVFEKDFYYVD INSAYPYAMKYPHAYGNRRAKSRHLPDERDPKIFVRLNCRSNGHFPIRTEKGALSFPD NGNVYEFHITGHEYYMAEDLELLHEVEILEVLTFEDEICFGDYVDHFYVMKEEATINK DTAKRLFAKLFMNSLYGKYATNPTKYKQYSVQNINTAEEFEYNNPDYECSTEFGDNLL YERDLYDDELKFLNVATAASITGYVRAFMMQALCSVKNPYYCDTDSIICEGLGDLEID ATKLGAWDIEAKATFCAIAGKKLYTMLCEDGSTKTASKGARLTHEQLIEVASGGEVLY KSEAPTFSVSRGVRFIERKIRKT" gene complement(3625..4200) /locus_tag="NVP1102O_07" CDS complement(3625..4200) /locus_tag="NVP1102O_07" /note="NVP1102O_07; Putative terminal protein" /codon_start=1 /transl_table=11 /product="putative terminal protein" /protein_id="AUR87642.1" /translation="MKDKEYKELFKALRPFVNFNIDLRKKLSSSDKRLITIYGNQFKD IYQGQNIKVFRTSNKNNLKVVQDAYHQTLKTLPKWKVAFVPYNGKQAPSIRVKDGIIK TYNKRSKLKQFIIPIVDQFSFLNDTFNYVSDLLEIHNVPPRQKLWVKTGDYLSTESFE AEILAEEMADWVERYENADTFITGFQSAFYR" gene 4214..4930 /locus_tag="NVP1102O_08" CDS 4214..4930 /locus_tag="NVP1102O_08" /note="NVP1102O_08" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AUR87643.1" /translation="MKTDDNQQLADLVDAVADSTIDDNTTLSENDESVNFQRIAEVTD IPESAETPEMPRELLFDENGEVRTDADGNTFDDSKHVVDENGHPKLTGKGKLRKKRGR KSGSISTTNAAQTAQKMAVSEKEKYRVVGAGAANALISLGIMLGGEDFAPIQNQHIDE KQNLESAFSDWAQTQEVDDIPPNLALTIVLGAYLLPRLTMPKQVSKLKLIKEWFKLKF FKKPKISKQTFDPNKESKED" gene 4933..5466 /locus_tag="NVP1102O_09" CDS 4933..5466 /locus_tag="NVP1102O_09" /note="NVP1102O_09; tara_best_match:OM-RGC.v1.021972741; InterPro:IPR002789; Putative packaging ATPase" /codon_start=1 /transl_table=11 /product="putative packaging ATPase" /protein_id="AUR87644.1" /translation="MPHCAIVGMTESGKSSLARKMAVELQKSGIQIIVFDPLSDPEWC NCLDLNNAFITDNENEFLNVYWNSKNCAVFFDEAGDYATNHNKPMIRTATKGRHWGHS NFYIAQRGNLLARTIRDQCANLYMFTSSKDDAKIYSTEFNDSELLNVPDLPQGTYYTC GRFKKAEKHILFKGKSK" gene 5463..5690 /locus_tag="NVP1102O_10" CDS 5463..5690 /locus_tag="NVP1102O_10" /note="NVP1102O_10" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AUR87645.1" /translation="MISLPLRFIGYLVTALLVETTISKTVIKKESLPDKEKKPNDSNE NKKPSNDGSDEKLAEVEDKGNGASDDESDSE" gene 5721..5816 /locus_tag="NVP1102O_11" CDS 5721..5816 /locus_tag="NVP1102O_11" /note="NVP1102O_11" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AUR87646.1" /translation="MLINPKAAKLALYVAISTYLFEAFIRPKIKG" gene 5927..6052 /locus_tag="NVP1102O_12" CDS 5927..6052 /locus_tag="NVP1102O_12" /note="NVP1102O_12" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AUR87647.1" /translation="MPKKYMPYLINAAVAGATAYLVTKATADNKTVVWYNPASWF" gene 6108..6962 /locus_tag="NVP1102O_13" CDS 6108..6962 /locus_tag="NVP1102O_13" /note="NVP1102O_13; tara_best_match:OM-RGC.v1.009973837; Double jelly roll capsid protein" /codon_start=1 /transl_table=11 /product="double jelly roll capsid protein" /protein_id="AUR87648.1" /translation="MAFRSNKVLPNFNGVGAGQTATLDVPIGSTYHQIAFEYSGMTTA QLTNFKVLLNGIPHIEIPSVEILEAISAFNGRGQTAGIFQLDFERFGLLTRQARELTA IGTGLGNTDPNQVRTFQIQVDIAAGAISPSLKATATTSPASFAGLVKKIRLFNRNPTG AGLFEISDLPKGDIINKVYFQAGAASSQINKLTLEIDNFVKFERSNALNDAIQKDDGI RVPQTKYSVFDPTELGYGAETVDTRYPMVEGNPRSGQNVSDMRFKLDMAAADNMLLLV EYLGTVER" gene 7032..7358 /locus_tag="NVP1102O_14" CDS 7032..7358 /locus_tag="NVP1102O_14" /note="NVP1102O_14" /codon_start=1 /transl_table=11 /product="TMhelix containing protein" /protein_id="AUR87649.1" /translation="MNIDSNPFENFFATTTPNQQQTGIWDSIIGAGSEIIGSLPDVVD GVIGNALNGKEQEKVDSNQNITSSDPSVSGDANKPSVMADNTGKYILYGFGGLITLVV VAKLIK" gene 7368..7520 /locus_tag="NVP1102O_15" CDS 7368..7520 /locus_tag="NVP1102O_15" /note="NVP1102O_15" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AUR87650.1" /translation="MPFLLPLAVLGGTGIFAWKAGEEVGSQTSNLLKIGAVGGGLYLL YKLQKG" gene 7524..7715 /locus_tag="NVP1102O_16" CDS 7524..7715 /locus_tag="NVP1102O_16" /note="NVP1102O_16" /codon_start=1 /transl_table=11 /product="TMhelix containing protein" /protein_id="AUR87651.1" /translation="MQAAGGGGMSASSAAGGAGDASGSSIGGVSIDLSGRGSEMNLTY IIGGAVVLGLAFIFMGKKK" gene 7717..8076 /locus_tag="NVP1102O_17" CDS 7717..8076 /locus_tag="NVP1102O_17" /note="NVP1102O_17" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AUR87652.1" /translation="MNKFELVSVEEVLAKMPKSIHQHVELVKEQMRENIQHGYKFNDL YVLFEPWDKTLMVQCVEGKGLNSEFVEALYKAAINNGFEYIQFVTPHKGLHRMVKELK PERVEYRYICKLSEVNL" gene 8073..8543 /locus_tag="NVP1102O_18" CDS 8073..8543 /locus_tag="NVP1102O_18" /note="NVP1102O_18" /codon_start=1 /transl_table=11 /product="coil containing protein" /protein_id="AUR87653.1" /translation="MSFSKGGSSKSATTNEQNPITATDSAIVAAEGSTVNITDGGAFD VVRDIIGSNESVVNDVILSNERISDTSINAGLDYFGQANASIQDSIDKVFDFANETVS QSQDTIASSAQAIFDSAKSDELNKSQDLNATIRELSKWAVFGVVGYTALRYAFK" gene 8555..9160 /locus_tag="NVP1102O_19" CDS 8555..9160 /locus_tag="NVP1102O_19" /note="NVP1102O_19" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AUR87654.1" /translation="MPIIREYVLNDLLPNQGNSKVINVTGRTIVIRRATNAGRVRVKA YTKEGGSLIADNIMDAGEKITTATEFTRLEVFNFEVDIRNVAITAGDGDFQSDVVEGE VTVKTDSSNPLVIASPKYVTSSSFIQHVSGTNSPLIPPAGTVEFGVQLQGADGAVVIE GGAIFLTKNQLYSSKFGDSSTAFIPDWAVSATYVVQWVAQD" gene 9170..9688 /locus_tag="NVP1102O_20" CDS 9170..9688 /locus_tag="NVP1102O_20" /note="NVP1102O_20" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AUR87655.1" /translation="MNLNPLFIIVPILGLYIMSRNSPSLHPSRGYRNNNPLNIRVSNS TWKGEVPANLNTDGSFEQFYDRANGYRAAAKLILNYNKLYNIFTVERIIARWAPAKGQ APDGQYYTNHTQNYIDYVVKNTSLDANSLINGSNVGELVWAMARFENLPPHTDSLEYV NESVSEFKDIDY" gene 9699..10088 /locus_tag="NVP1102O_21" CDS 9699..10088 /locus_tag="NVP1102O_21" /note="NVP1102O_21" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AUR87656.1" /translation="MFLSAPRINPLKVATPLAVGGIVQLPNPAPKQWLRMDTIIPAHF GCIVITVGRHYSSAPAENGFFIERVSDGYRLYEINPFAAANTYDEASYTIQRPLEGWA GYNFLSNTSGAGTINITAVPQALDLYS" gene 10098..10310 /locus_tag="NVP1102O_22" CDS 10098..10310 /locus_tag="NVP1102O_22" /note="NVP1102O_22" /codon_start=1 /transl_table=11 /product="TMhelix containing protein" /protein_id="AUR87657.1" /translation="MTNANTQGKKFNVIAALIAALSVGFGSSQMTFNYSPPDEMQMIE CITVIDKLVTTHNGDIKAVVQMLTAD" ORIGIN 1 cttttcgctc gtacgtcaca ctttagacat ttaaatttga caacttatgg aatatatgaa 61 acgaaaaaag acccgcgtta gcgggtcgat tgagttgaat ggaatgcagc gaagcgggtt 121 aatcgtttga aagctctata atctttaaac taactttttc taatgcaata acatttgcgt 181 ctatccaata tttatctgga gtgtccgtcg taagctcaaa atatgaatga gggtgtatcg 241 gcattttata ttcgcctcga cataaataca caaacactct tttattataa ttcgaaactc 301 ttataacgtg agttgggtca ttagttttag tagccatatt catttgctct cttgaatggg 361 gaattgaatg cgcattacgc acctttgtaa tgaattaatg aacgtcgaat ctgacaccaa 421 taaagtttaa caccgctctc ataaggcaat ggttcggggt caagctcgta atggtcgccc 481 cattggtcat gcatatattc gataatatcg tcctcgctct cgtccatcca gttagtgata 541 agatataaaa cgtttgtgag ttttgattta acgtatctag ccatgattag aactccaagt 601 aaaaagcgat gttagcgacg tatagattcc atacactgat tcgcgcacca atggattcat 661 atacagtgat atcaacaaaa ggagcaacaa taggagcgaa accgttcgag ttatatttag 721 ctttttcgta accatccgca attccgcctt gcgcgccaac tgcaagccca atatcgttgt 781 cgaacctgta atcgcgccaa gtataaacga ttgagagcgc gttagattgg acgttgtaag 841 agtttgtgaa atttgtgaaa gcgattgcgt aattttctgt gattttgtat tcgaatccat 901 aagttctgtg aacctcgtta taatcatatg aacggtcgaa gtgatacgag tacgcatcac 961 ccataaaaat tttggtctta ccctcaagcg gttctagaac tgtaggaagt ccgaactcgc 1021 taaagtccaa ccaagtgccc gaagtatcta aactttccgg ctcagtttct gacgccccga 1081 caggggaagc gatgcaaaga actaaaacga acgacaaagc tactccatga taacccgccg 1141 ccgctagtcg atgcgccgcg taaagaatac aaagtcccaa gcaaaaataa atagtcataa 1201 tcattgtaaa actcctattg aattacacaa agagcggcga aacctgccgc gcccactaaa 1261 tgaaatagaa tcataaacac gatatctgta atgtttcggt tagcttcgtt cccaaccatc 1321 gcgatacaga tagagctaag tcctaacact aaaatcacgc tcaatgtaaa cattggtctt 1381 actcccaaca aaaagggcgt atcggatttg ccgccgatac gccctaagtt ctaattttaa 1441 aatttccagt tgatagcttg aatttctgat tggggtaaca acgaaacaag gctctcaagc 1501 ttggctttgt ttacgccttt gagggtgaca acttcaccac cctcaagagt cactttacaa 1561 gtaatcaatg ttacgcgact tcgatttcgt aaattgtgtg ctcagggaaa cgcttacctt 1621 gaactttacg agtcacgact tcgtaagctt tgcccgcttc gaacttcgcc gctagacctt 1681 tgtgaagata gatgtcgaac tgctcaccgc tttctagctc agttacggtt gatttcatca 1741 tatccggttt agcatcttct tttgcgctta cgtggtcaac gaacgccttt tcgattttga 1801 taaagtgaga ctttgccgcg ccgtctttgc cataatcaag agaaagaaga ggaagagaaa 1861 cagccgcgat acgtttaaaa gtagggaaag tttttgcttg cttagtcata atatagctcc 1921 tagcccgtag gctgtaaaat taaaggctta acgccgtttt ggggcttatc ccctgaaaga 1981 gattacatag taaagaatct ctttcgtgtt gtaaactact ttttcacgtt tttctgattt 2041 ttctttcaat gaagcgaact ccacgcgaaa ccgaaaacgt tggtgcttcg cttttatata 2101 gaacttcgcc gccgctcgca acttcgatta gttgctcatg cgtgagcctt gcgccctttg 2161 aggcggtttt cgtgcttcca tcttcgcaaa gcatcgtata aagcttttta cccgctatag 2221 cacaaaaggt cgcctttgct tctatatccc acgcgcctag tttggttgcg tcgatttcta 2281 aatcacccaa accctcgcag ataatcgaat cagtatcgca gtaatacgga ttcttaacac 2341 tgcataaggc ttgcatcata aacgctcgca catatccggt aatgctcgca gcggtcgcca 2401 cgttcaaaaa ctttagttca tcgtcgtaaa ggtcacgctc gtataaaagg ttatcgccga 2461 actcagtcga gcattcatag tcgggattgt tatattcgaa ctcttccgcc gtattgatgt 2521 tttgaactga atactgttta tacttcgtcg ggttcgtcgc gtacttaccg taaagactat 2581 tcatgaacag cttcgcgaac aatcgtttcg ccgtatcttt atttatggtc gcctcttctt 2641 tcatcacgta aaagtggtca acgtagtcac cgaaacaaat ctcatcctcg aacgtcaaca 2701 cttctaaaat ctcgacttcg tgtaaaagct ccaaatcttc cgccatgtaa tattcatgcc 2761 cagtgatatg gaactcgtaa acgttcccgt tgtcagggaa agagagcgca cccttttcag 2821 tcctaatcgg gaaatgacca ttcgaacgac aattcaaacg aacaaaaatt ttagggtctc 2881 gctcatctgg caagtggcgc gacttcgctc ttctattccc gtaagcatgg ggatatttca 2941 ttgcgtaagg atacgcggaa ttaatatcca catagtaaaa gtccttttca aaaactcccg 3001 tcttaaacgc ttgaactctt ccaccaaaat agaagcgatt caaattctcg aaatagttta 3061 tattggttct aggcgttttc aatcccgcca tattattaaa aacttttaaa gccgtacttg 3121 cgagcgtgat attaaatccg tagtcagtga caaaacgagt aaccaactca tgcaaataaa 3181 cgcagtcact tttcaagtaa tcgcgtatcg ctttccagtt ttccggttta tatctctctt 3241 ccttttcaaa caggtcatat gatatttcat ccttttgata cgccgcgagc ggaataggta 3301 aaatattata tgagtctcta aaagtacatt taccgatttt aaactcagcg atacgccccg 3361 cgataatttt aatgtctggt tttatttcgc attcccacaa aacaaaatgc cagtcgaaac 3421 gtccgccgtt atgagcgtaa atagtccact cttcgtctcg aacgaaatta acgaaatcaa 3481 ttgttttctc gaactcataa tattgttcat taatatatag accccacaaa aacggctttg 3541 gaactcgacc atatttaaac gggtcggttt cgcaatccgc cgcgcctata ctttctaaat 3601 cgtttcgctt cttacccatt ttaattacct ataaaaagca gattggaaac cagtaataaa 3661 agtatctgcg ttctcgtaac gctcaaccca atccgccatt tcttccgcta atatctcagc 3721 ctcaaaactt tcggtagaaa gataatcacc agttttaacc caaagctttt gtcttggcgg 3781 tacgttatga atttctaata agtcgcttac ataattgaac gtatcattta agaatgaaaa 3841 ttggtcaacg attggaataa taaattgttt aagtttagag cgtttattat atgttttaat 3901 aatcccgtcc ttaactcgaa tgctaggagc ctgtttaccg ttatatggga caaatgcgac 3961 tttccactta gggagcgtct ttaaagtctg gtggtacgcg tcctgtacta cttttaaatt 4021 gtttttatta gatgttctaa atacttttat attttgacct tgatatatat ctttgaattg 4081 atttccatat attgtaataa gtcttttatc gctagaactt agctttttgc gtaaatcaat 4141 gttaaaatta acaaatgggc gcaatgcctt aaataattct ttatactctt tatctttcat 4201 gggagcacct taaatgaaaa ctgacgataa tcaacaatta gccgatttag ttgatgcggt 4261 agcagattca acaatcgacg ataacactac cttatcagaa aatgacgaaa gtgtcaattt 4321 tcaaagaatc gcagaagtta cggatattcc agaatcggcg gaaacccccg aaatgccgcg 4381 agagctatta ttcgacgaaa atggcgaagt acgcacagac gccgacggta atacattcga 4441 tgattctaaa catgttgtag atgaaaacgg gcaccctaag ctgacaggaa aaggtaagct 4501 ccgcaaaaag cgcgggcgca agtcgggctc gatttctacc actaacgccg cgcaaactgc 4561 tcagaaaatg gcggtaagcg aaaaggaaaa atatcgagta gtcggagcgg gcgcggcgaa 4621 tgctttaatc agcctgggaa ttatgttagg aggcgaagac ttcgcgccaa ttcaaaacca 4681 acatatagac gaaaagcaga acctagaatc ggctttttcc gattgggcgc aaactcagga 4741 agtggacgat attccaccaa acctagcatt aacaatcgtg ttaggggctt atttgctacc 4801 gcgcttaaca atgccaaaac aggtaagcaa attaaaacta attaaagaat ggtttaaatt 4861 aaaattcttt aaaaagccga aaatttcgaa acaaactttt gacccaaata aagaatcaaa 4921 ggaggattaa tcatgcctca ttgcgctatt gtaggaatga cagaaagcgg caaatccagt 4981 ttagctcgta aaatggcggt agaacttcaa aagagcggta ttcaaataat tgtatttgac 5041 ccgttaagcg accccgaatg gtgcaactgt ttagatttaa ataacgcttt tattaccgat 5101 aatgaaaacg aatttttaaa tgtatattgg aactcgaaaa actgcgcggt atttttcgat 5161 gaagcgggag actacgcaac taatcataat aagcccatga ttagaaccgc cacaaaagga 5221 cgtcactggg gtcattctaa tttttatata gcgcaaaggg gtaatttatt agcgcgtact 5281 attcgcgacc aatgcgctaa tttatatatg tttacctcat ctaaggatga tgctaaaata 5341 tatagtacag aatttaatga tagcgaatta ttaaatgtac ctgatttgcc tcaaggaacg 5401 tactatactt gtggtagatt taaaaaagct gaaaagcata ttttatttaa ggggaagtca 5461 aagtgatttc attaccatta cgttttatcg gttatttagt taccgcttta ttagttgaaa 5521 caactatttc aaaaacggta attaaaaaag agtcattacc tgataaggag aaaaagccaa 5581 atgacagcaa tgaaaataaa aagccgtcga atgatggaag cgatgaaaaa ctcgcagaag 5641 tcgaagacaa aggaaacgga gcaagcgacg acgaaagtga tagcgagtaa cgtttataat 5701 tctaactagg ggtaataaac atgctaatta atcctaaagc cgctaaactt gctttatacg 5761 ttgcgattag cacttattta tttgaggcgt ttattcgacc aaagataaaa ggttagctca 5821 atgagtatta cagacttttt taaaatcgcg ggtatttcgt tgttaacaac gtatgccatt 5881 aatcaatcgc ctttcaagcg atactttagt taaacggaga tacaccatgc caaagaaata 5941 tatgccttac ctaatcaacg cggcggtagc gggtgcaact gcttaccttg taaccaaagc 6001 gaccgctgat aataaaacgg ttgtatggta caatcccgct tcttggtttt aattaacctc 6061 gacactttca agcatttaat tcatttagtt actaggagat aaaagccatg gcttttcgtt 6121 caaataaagt tttacctaat ttcaacggcg tcggcgcggg tcagactgct acgctagacg 6181 tgccgattgg ttctacttac caccaaatcg cgtttgagta ttctggtatg actaccgcgc 6241 aacttacaaa ctttaaagtc ctactgaatg gtatccccca tattgagatt ccatcagttg 6301 aaattcttga agctattagc gcgtttaacg gtcgcggtca aacagcgggc attttccaac 6361 tcgactttga gcgttttggt ttgcttactc gtcaagctcg cgagcttaca gcgattggta 6421 cgggcttagg taatactgac cctaatcaag tgcgcacgtt ccaaattcaa gttgacattg 6481 cagcgggcgc aatctcgccg agcctaaaag ctaccgctac aacttctccg gcatcttttg 6541 cgggattggt taaaaagatt cgtctattta accgaaaccc aacgggtgcg ggtctattcg 6601 aaatttctga tttgccgaaa ggcgatatta ttaacaaagt ttactttcaa gcgggcgcgg 6661 cctcgtcgca aatcaacaag ctcacacttg agattgacaa cttcgttaaa ttcgaacgtt 6721 cgaacgctct aaacgacgca atccaaaaag atgatggtat ccgtgttcct caaactaaat 6781 actcagtatt tgacccaacg gaactaggtt atggcgcgga aaccgtggat acacgctacc 6841 caatggtcga aggcaatcca cgaagcgggc aaaacgtttc ggatatgcgc tttaagttgg 6901 atatggcggc ggcggataac atgctcttac tcgttgagta tcttggaacc gttgagcgtt 6961 aatgacgctg taacatttta aatctaaacc atagggcggg ctaatgctcg ccctttttgg 7021 agaaaaacat tatgaacatc gattcaaatc cgtttgaaaa ctttttcgct acaaccactc 7081 caaaccaaca acagactgga atttgggatt caattatcgg cgcaggttct gaaattatcg 7141 gctcattgcc ggatgttgtt gacggcgtaa tcggtaacgc tctaaacgga aaagagcaag 7201 aaaaggttga ttcaaaccaa aacattacct caagtgaccc gagcgtttcg ggcgatgcta 7261 acaaacctag cgttatggct gacaataccg gaaaatacat tttgtatggc tttggcggtc 7321 tcatcacttt ggttgtagta gccaaactaa ttaagtaagg agttgttatg cctttcttat 7381 tacctttggc ggtgttgggt ggaactggga ttttcgcttg gaaagcgggt gaggaagtgg 7441 gttcacaaac ctcaaacctt ttaaagattg gtgcggttgg cggcggtctt tatcttcttt 7501 ataaacttca aaagggttaa attatgcaag cagcgggcgg gggcggaatg agcgcgtcga 7561 gtgcggcggg cggtgcgggc gatgcgtcgg gctctagtat cggcggcgtt agtatcgatt 7621 tgagtgggcg cgggagtgaa atgaatctca cgtatattat aggcggggcg gtagtattgg 7681 ggctcgcttt tattttcatg gggaaaaaga aatagcatga ataaatttga acttgtttcc 7741 gttgaggaag tattagcaaa aatgccaaaa agtattcatc agcacgttga actagtaaag 7801 gagcaaatgc gcgaaaacat ccaacacggt tataagttca atgatttata tgttcttttt 7861 gaaccgtggg acaaaacctt aatggttcaa tgcgtagaag gtaaaggttt aaattccgaa 7921 tttgttgaag ctttatacaa agccgctatc aataacggat ttgaatacat tcagttcgtt 7981 acgccgcata aaggcttaca cagaatggtt aaagagttaa agcccgagcg tgttgaatat 8041 cgttatatct gcaaactcag tgaggtgaat ctatgagttt ttcaaagggc ggctcttcta 8101 aatccgctac cactaacgag caaaacccaa tcacggcgac agattccgct attgtcgccg 8161 ctgaaggctc aacagttaat attaccgacg gtggggcgtt tgatgttgta cgcgatatta 8221 tcgggtctaa cgaaagcgtc gttaacgacg ttattctaag caacgagcgt atttccgata 8281 cttcgattaa cgcggggctc gattatttcg gacaagccaa tgcatcgatt caagatagta 8341 ttgacaaggt ttttgacttc gcgaatgaaa ccgtatctca atcacaagat accattgcga 8401 gtagcgcgca ggccattttt gattctgcga aatcggacga acttaacaag tctcaagact 8461 taaacgcaac aatccgagaa ctttcaaagt gggctgtttt tggggtcgtg ggttacactg 8521 cgctacgtta cgcatttaaa taggagtttg taaaatgcca attattcgag aatatgtttt 8581 aaatgacttg ttaccaaatc agggtaattc aaaggttatc aatgttacag gccgcacaat 8641 tgtgattcgt cgcgctacca acgcggggcg tgttcgcgtg aaagcttaca ctaaagaggg 8701 cggttcgctt atagctgaca acattatgga tgcgggagaa aagattacaa ccgcgaccga 8761 atttacacga ctagaagtgt ttaactttga agtcgatatt cgaaatgttg ctattacggc 8821 gggtgacggc gattttcaat ctgatgttgt tgaaggtgaa gttactgtca aaactgattc 8881 ctctaacccg ttagtaatag cctccccaaa gtacgttact agctcatcct ttattcaaca 8941 cgtctccggt acgaattcac cgttaattcc cccagcgggc acagtggagt tcggtgttca 9001 actacagggc gcagacggag cggtagtgat agagggtgga gctatattct taaccaaaaa 9061 ccagttgtat agctccaagt tcggcgatag cagtaccgcg tttatcccag actgggctgt 9121 gagcgcgaca tacgttgtgc agtgggtagc tcaggattag gagcgattta tgaatctcaa 9181 ccctttattt atcatcgtac caattttagg actttatatt atgtctagaa attcgccaag 9241 tttacatccc tcgcgtgggt atcgtaacaa caatccttta aatatccgag tgtctaactc 9301 aacttggaaa ggggaagtac ccgccaattt aaacactgac ggctcgttcg aacaatttta 9361 cgaccgagca aatggatatc gcgccgccgc gaaattgatt ttaaactata acaagcttta 9421 taacattttc actgtcgagc gtattattgc gcgatgggct cccgctaaag gtcaagctcc 9481 tgacggacaa tattacacaa accacactca aaattatata gactacgttg ttaagaatac 9541 gtcgcttgat gcgaattcgc ttattaacgg gtcaaatgtt ggtgaattag tgtgggctat 9601 ggctcgcttc gaaaacttgc cgccgcatac cgattcgctt gaatacgtta atgaatctgt 9661 tagcgaattt aaagatatcg actactagga gaaagattat gtttttatca gctccaagaa 9721 tcaatccatt aaaagtcgct accccgttag cagtgggagg aatcgtacaa ttacccaatc 9781 ccgcacctaa gcagtggttg agaatggaca ctattatacc tgcccatttc gggtgtatag 9841 taattacggt tgggagacat tactcgtctg cgcccgccga aaatgggttt tttatagagc 9901 gagtttctga tgggtatcgc ctgtacgaaa ttaacccttt cgctgccgct aatacatatg 9961 acgaggcaag ttatacaatc caaagaccac tagagggatg ggcgggctat aattttctca 10021 gcaatacaag cggcgcgggc acaataaata tcactgccgt accccaagcg ttagacctat 10081 attcataagg aaatactatg acaaacgcaa atactcaagg taaaaagttc aacgtaatcg 10141 ctgctttaat cgctgcgctc agtgtcgggt ttgggtcgtc ccaaatgacg tttaattatt 10201 caccgcccga cgaaatgcaa atgattgaat gtatcactgt aatcgataaa cttgttacta 10261 cgcataatgg ggatatcaaa gcagttgtcc aaatgctaac cgctgactaa tctcaaccct 10321 ttcgggcgtt tgaattgtgc tcctttcatt cgcccgcttt tctaaatgtc aagccctttt 10381 tctacgtttc atatattcca taagttgtca aatttaaatg tctaaagtgt gacgtacgag 10441 cgaaaag //