LOCUS MG592500 10578 bp DNA linear VRL 24-JAN-2018 DEFINITION Vibrio phage 1.125.O._10N.286.49.F5, partial genome. ACCESSION MG592500 VERSION MG592500.1 DBLINK BioProject: PRJNA328102 KEYWORDS . SOURCE Vibrio phage 1.125.O._10N.286.49.F5 ORGANISM Vibrio phage 1.125.O._10N.286.49.F5 Viruses; dsDNA viruses, no RNA stage; unclassified dsDNA viruses. REFERENCE 1 (bases 1 to 10578) AUTHORS Kauffman,K.M., Hussain,F.A., Yang,J., Arevalo,P., Brown,J.M., Chang,W.K., VanInsberghe,D., Elsherbini,J., Cutler,M.B., Kelly,L. and Polz,M.F. TITLE A major lineage of nontailed dsDNA viruses as unrecognized killers of marine bacteria JOURNAL Unpublished REFERENCE 2 (bases 1 to 10578) AUTHORS Kauffman,K.M., Hussain,F.A., Yang,J., Arevalo,P., Brown,J.M., Chang,W.K., VanInsberghe,D., Elsherbini,J., Cutler,M.B., Kelly,L. and Polz,M.F. TITLE Direct Submission JOURNAL Submitted (29-NOV-2017) Department of Civil and Environmental Engineering, Massachusetts Institute of Technology, 15 Vassar Street, Bldg. 48-417, Cambridge, MA 02139, USA FEATURES Location/Qualifiers source 1..10578 /organism="Vibrio phage 1.125.O._10N.286.49.F5" /mol_type="genomic DNA" /isolation_source="iron chloride precipitation of 0.2um-filtered seawater, resuspended in oxalate solution" /host="Vibrio splendidus" /db_xref="taxon:1881427" /lab_host="10N.286.49.F5" /country="USA: Massachusetts, Nahant, Canoe Cove" /lat_lon="42.419 N 70.906 W" /collection_date="2010-10-13" /collected_by="Kathryn Kauffman" gene complement(129..320) /locus_tag="NVP1125O_01" CDS complement(129..320) /locus_tag="NVP1125O_01" /note="NVP1125O_01" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AUR89572.1" /translation="MIISINNWSGGKVKIFNRTSGKVIRLEDGESETVETAAITSLII LRCILANSHLILFDIENTD" gene complement(322..528) /locus_tag="NVP1125O_02" CDS complement(322..528) /locus_tag="NVP1125O_02" /note="NVP1125O_02" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AUR89573.1" /translation="MARYVKSKLTGVLYLITNYMEDDADDIIEYMHDQWGDHYELDPV PLPHEGGVKLYWCQVRQSLIHLKE" gene complement(528..746) /locus_tag="NVP1125O_03" CDS complement(528..746) /locus_tag="NVP1125O_03" /note="NVP1125O_03" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AUR89574.1" /translation="MARMFQIRNHGEESVTMIHAVADTASFKAQMVELEAGAFVVMPE PELENYALPLSLGAKVNLVTVTELERVQ" gene complement(749..1366) /locus_tag="NVP1125O_04" CDS complement(749..1366) /locus_tag="NVP1125O_04" /note="NVP1125O_04" /codon_start=1 /transl_table=11 /product="TMhelix containing protein" /protein_id="AUR89575.1" /translation="MVMTIYFCLGLCILYAAHRLAAAGYHGIALAFVLVLCVASHVGA VETEPESLDTSGTWLDFSEFGLPTVLEPLEGKTKIFMGDAYSYHFDRSYDYNEVHRTY GFEYKITENYAVAFTNFTNSYNKQSNALSIVYTWRDYRLDNNIGLTVGAQGGIADGYE KAKYNSNGFAPIVAPFVDITVYESIGARISVWNLYVANIAFYLEF" gene complement(1563..1895) /locus_tag="NVP1125O_05" CDS complement(1563..1895) /locus_tag="NVP1125O_05" /note="NVP1125O_05" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AUR89576.1" /translation="MAKSTQPTFKRVATVSLQMFSLDYGKDGESKSHFIRIVKAFAEH VSAKEDAKPDMTKGVITELVTGEQFDLYLHKGLAAKFEEGKAYEVVTRKPKGKRFPEH TIYEIEVA" gene complement(2004..3608) /locus_tag="NVP1125O_06" CDS complement(2004..3608) /locus_tag="NVP1125O_06" /note="NVP1125O_06; aclame_best_match:protein:vir:2124; tara_best_match:OM-RGC.v1.001450554; cvp_best_match:RF_PEP_9626351; InterPro:IPR004868; pfam:PF03175|pog:POG0334" /codon_start=1 /transl_table=11 /product="DNA polymerase type B" /protein_id="AUR89577.1" /translation="MTRKANRRLLENIGAADCETDPFKYGRIPKPFIWGLYINEQYYE FEKTIDFINFVRDEEWTIYAHNGGKFDWHFVLQECEIKPSVKIIAGRIAEFQIGKCTF RDSYNILPIPLAAYQKDEIDYALFEKETRYEPETWKAIRAYLKSDCVYLHELVTAFVS EYGFNITLASTALKVFNQMSNLKTPRTNLNYFERISPYYFGGRVQAFKTGTFEKDFYY VDINSAYPYAMMYDHAYGNKRTKSRHLPDEKDVKIFVTLKCKSNGHFPVRLENGSLSF PDDGNIYEFKVTGHEYYAAEELGLLEDVEIIEVLTFEDSICFANYVEHFYVMKEQATI EKDEAKRLFAKLFMNSLYGKYATNPTKYKQYSMQSVHTAEEFENFNPDYVCATEIGDN LLYERDLYDDELKFLNVATAASITGYVRAFMMRALCSVDTPYYCDTDSIICEGLGDLE IDATKLGAWDIEGEASFCAIAGKKLYSMVLKDGKTKTASKGARLTHDELVEVAQGGEV VWKSDAPTFSISRGIRFNERKIRKTA" gene complement(3615..4190) /locus_tag="NVP1125O_07" CDS complement(3615..4190) /locus_tag="NVP1125O_07" /note="NVP1125O_07; Putative terminal protein" /codon_start=1 /transl_table=11 /product="putative terminal protein" /protein_id="AUR89578.1" /translation="MTEKEYKELFKIIKPYVNFNIDLRKKLSIGDKRLITKYTNQFKE LYQGMNIKIFRSSNKANLKTVQNTYHQVFKTLPRWKVAFVPYSGSQAPSIRVKNGIVK TFNKRSKLKQFIIPIENQGEFLLNTREYVQGLLDKHNVPDRQKLWVKTGDYLSTESFE AELLADEMEDWVNRYENAETFITGFQSAFFR" gene 4207..4935 /locus_tag="NVP1125O_08" CDS 4207..4935 /locus_tag="NVP1125O_08" /note="NVP1125O_08" /codon_start=1 /transl_table=11 /product="coil containing protein" /protein_id="AUR89579.1" /translation="MNTSDIVADVENSFLDNLDTLPESGTNGKFERTSEIGNFSESVE TVETVETVEDSAPSEIPRHLLWDESGNERADADGNTFDLSLHSVDDNGHPKLTAKGKL RKKRGRKGASTLSSSTASNKVAQVAISEKAKFQAVGAGAANALICLGLAIGGEDFAPI RNADMDEKSNLEKAFADWAETQEVEDIPPNLALTIVVGSYLFPRLVMPRQVTKFQMLK QWLKLKFFKKPTAAKVFDPNKEEN" gene 4935..5468 /locus_tag="NVP1125O_09" CDS 4935..5468 /locus_tag="NVP1125O_09" /note="NVP1125O_09; tara_best_match:OM-RGC.v1.021972741; InterPro:IPR002789; Putative packaging ATPase" /codon_start=1 /transl_table=11 /product="putative packaging ATPase" /protein_id="AUR89580.1" /translation="MAHSAIVGMTESGKSSLARKMATAIQKAGIAVIVFDPLGDPAWS ECLDPNNSFITDNEKEFLNYYWSSKNCAVFFDEAGDYATNHNKEMIRTATKGRHWGHS NYYIAQRGNLLARTIRDQCSKLYMFTSSKDDAKIYSLEFNDNELLNVPDLSQGEYYSC GRFNKAVKHILFKGKSK" gene 5553..5804 /locus_tag="NVP1125O_10" CDS 5553..5804 /locus_tag="NVP1125O_10" /note="NVP1125O_10; aclame_best_match:protein:vir:105367; tara_best_match:OM-RGC.v1.010270429; cvp_best_match:NCBI_PEP_448930578; kegg:none|pfam:PF08017|cog:COG1652|pog:POG0623" /codon_start=1 /transl_table=11 /product="fibrinogen binding protein" /protein_id="AUR89581.1" /translation="MLMKLKLRRIRQNQKLKKIQVAKAEAEAKAKAEAEAKTQAEAKA KAEAEAKTQAETKAKAEAEAKQAANEALKNAQKSATVGE" ncRNA 5652..5671 /ncRNA_class="scRNA" /locus_tag="NVP1125O_10" /product="NVP1125O_CRISPR_spacer_1" /note="CRISPR spacer 1" ncRNA 5694..5713 /ncRNA_class="scRNA" /locus_tag="NVP1125O_10" /product="NVP1125O_CRISPR_spacer_2" /note="CRISPR spacer 2" gene 6040..6165 /locus_tag="NVP1125O_11" CDS 6040..6165 /locus_tag="NVP1125O_11" /note="NVP1125O_11" /codon_start=1 /transl_table=11 /product="TMhelix containing protein" /protein_id="AUR89582.1" /translation="MPKKYMPYAINAIVAATTAYLVTSAVAKQKQVVWYNPTTWL" gene 6222..7067 /locus_tag="NVP1125O_12" CDS 6222..7067 /locus_tag="NVP1125O_12" /note="NVP1125O_12; tara_best_match:OM-RGC.v1.009973837; Double jelly roll capsid protein" /codon_start=1 /transl_table=11 /product="coil containing protein" /protein_id="AUR89583.1" /translation="MSFRSNKVLPNFNGVGAGQTATLDVPIGSTYHQIAFEIAGVSPQ ELKNFKVLLNGIPHIELPSMSTLAEMNEFDGIGGAMGVLILDFERFGLLTRQARELTA IGTGMGDADPNQVRTFQIQMDIDPAATNPSIRATATTSPASMSGLIRKIRLFNKTPTG SGLFEISDLPKGDMINKVFFVAQEDTKIDKLTLELDNFVKFERTAGLNDVLQMNAGVR VPNPKMYVLDPTEMGYGAEVIDTVYPAGHPREGQNLSDVRFKLNMSGGQGQLVIVEYL GAVER" gene 7145..7471 /locus_tag="NVP1125O_13" CDS 7145..7471 /locus_tag="NVP1125O_13" /note="NVP1125O_13" /codon_start=1 /transl_table=11 /product="TMhelix containing protein" /protein_id="AUR89584.1" /translation="MNVDSNLFENWFGTTSAQGQQTSLWDKLVNAGGQVIDTLPDIVD GVVDSKLNAEIKNKTETTENITSTNPSVSGSANQLTFLKDPKVMYGGLAIGGIIAAAI LIKALK" gene 7485..7634 /locus_tag="NVP1125O_14" CDS 7485..7634 /locus_tag="NVP1125O_14" /note="NVP1125O_14" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AUR89585.1" /translation="MPFLLPIAVLAGTGVFAWKAGDAVNNVSDKLVSAAIIGGAAYLI YKGSK" gene 7637..7825 /locus_tag="NVP1125O_15" CDS 7637..7825 /locus_tag="NVP1125O_15" /note="NVP1125O_15" /codon_start=1 /transl_table=11 /product="TMhelix containing protein" /protein_id="AUR89586.1" /translation="MQAAGGGGMSAGSSAGAVGGASGSSIGGVNIDLSGRGDMNVTYL VGGALAVGALFIFLTKRK" gene 7834..8193 /locus_tag="NVP1125O_16" CDS 7834..8193 /locus_tag="NVP1125O_16" /note="NVP1125O_16" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AUR89587.1" /translation="MNKFEPVPIDEILEAMPKSIHRHTDLVKRQLKAQHQYGYKFRGL YIIFEPWDDTLMVQCVEGTGLDEDFVKALYKCAINNGFEYIQFVTPHKGLHKMVKSLK PERIEFRYICKLDEVEL" gene 8190..8681 /locus_tag="NVP1125O_17" CDS 8190..8681 /locus_tag="NVP1125O_17" /note="NVP1125O_17" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AUR89588.1" /translation="MSFSRGGSSESATTNEQNPITATDNAMVASEGGTITVTDGGAFN VVTDAIDAIGDVVLSAESLAKNAMSSTESIASDSINAGLDYHSQTTGFLEGTLDKVFN FVGDTVQKSQDTISGNTQALFNSAKSDSLNRSEDMQQTIRTLGMYATIGLVGYTAFRF LKK" gene 8691..9296 /locus_tag="NVP1125O_18" CDS 8691..9296 /locus_tag="NVP1125O_18" /note="NVP1125O_18" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AUR89589.1" /translation="MSLIRDYDLNIESNATKSFATTGTMIAVRRGDDPIRVRVKAYTK EGGTLVTDSVLSAGEKVRTGEEFNFIEITNYELTKRLVRVTLGDGDFQSDVVEGEVVV RSDTDNPLQVISKRAPRDVLSAFITHQKGTDTPVTSPDGTIKFSAQNLTGGDIYIFIG NAYIYLAPTQLFSTDIAQDTRIKVAGISEGGQISMFWVIEG" gene 9354..9821 /locus_tag="NVP1125O_19" CDS 9354..9821 /locus_tag="NVP1125O_19" /note="NVP1125O_19; tara_best_match:OM-RGC.v1.019721584" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AUR89590.1" /translation="MSKRSPALHASRGYRNNNPLNIRIGSSKWKGEIPTDKNTDGKFE QFYKRSDGYRAAAKLILNYNRLYNIFTVQRIIERWAPAIGNAPDGTKYKNHTHQYIDF VCRKTGFDPNTLINGSNVGELVWAMAQFENMPPHTDSLDFVKNSVSEFKDINY" gene 9830..10222 /locus_tag="NVP1125O_20" CDS 9830..10222 /locus_tag="NVP1125O_20" /note="NVP1125O_20" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AUR89591.1" /translation="MFLSAVPQKKVNPARIQQTLSASNMVSVTIPAKTWRVMSADVPD NVATLVFVITRDYSSAPADTGVTIERVSDGHTVYEATVSDSPNTSEYATLQVSRPYEG WGSLKIYNNTANAGRLIINAWTNTDLYA" gene 10232..10453 /locus_tag="NVP1125O_21" CDS 10232..10453 /locus_tag="NVP1125O_21" /note="NVP1125O_21" /codon_start=1 /transl_table=11 /product="TMhelix containing protein" /protein_id="AUR89592.1" /translation="MPNVHPKKFNVIAGILAGALVGFGGGSVNLNYNASPNELQLLDC LTTIDNMIKSHDDNLGMVVQSLMESKIQS" ORIGIN 1 cgcatatttg cggatataca tcacatttat gagatttaaa tttgacaaga aaggggaagt 61 gtgtaagagg cgcgccgcac tcaggcgaag ccgtgaaagg agtgaaacga aaaaagcccc 121 cgtaggggct aatcagtgtt ttcaatatcg aaaagtatta aatgcgaatt ggcaagtata 181 cagcgtaaaa taatcaagga tgtgatagcg gcagtttcta ccgtttcgct ttccccgtcc 241 tctaatctaa tgaccttacc cgacgttcga ttaaatattt taacctttcc gcccgaccaa 301 ttattgattg atataatcat gctattcctt taggtgaatc aaactttgtc gcacttggca 361 ccaatacaat ttaaccccgc cttcatgcgg aagcggtacg gggtcaagtt cgtaatggtc 421 gccccattgg tcatgcatat actcgatgat atcgtctgcg tcgtcttcca tgtaattagt 481 gatgagatac aaaacgcccg ttaattttga ttttacatat ctagccatta ctgaaccctc 541 tccaattctg taacagtaac taagtttact ttagcaccca acgatagcgg taaagcgtag 601 ttctccaact ctggctctgg catcaccacg aacgcgccag cttccaactc aaccatttga 661 gctttaaacg acgcagtgtc cgcaactgcg tgaatcatcg taactgattc ctcgccgtgg 721 tttcgaattt gaaacattct agccatttct agaactccaa gtaaaaagcg atgttagcaa 781 cgtatagatt ccaaacactg attcgcgcac caatggattc atatacagtg atatcaacaa 841 aaggagcaac aataggagcg aaaccgttcg agttatattt agctttttcg taaccatccg 901 caattccgcc ttgcgcacca actgtaagcc caatattgtt gtcgagcctg taatcgcgcc 961 aagtataaac gattgagagc gcgttagatt gcttgttgta agagtttgtg aaatttgtga 1021 aagcgactgc gtaattttct gtgattttgt attcgaatcc ataagttctg tgaacctcgt 1081 tataatcata tgaacggtcg aagtgatacg agtaagcatc acccataaaa atctttgtct 1141 ttccctcaag cggctctaga actgtaggaa gtccgaactc gctaaagtcc aaccaagtac 1201 ccgaagtatc taaactttcc ggctcagttt ccaccgcccc gacatgggaa gcgacacaaa 1261 gaactaaaac gaacgccaaa gctattccat gataccccgc cgccgctagt cgatgcgccg 1321 cgtaaagaat acaaagtccc aaacaaaaat aaatagtcat aaccattgta aagctcctaa 1381 tgcaaaaggg cgtatcagat ttgccgctga tacgccctaa gttctaattt tttaatttcc 1441 aattgattgc ttgaatttct gactgtggta acaaggaaac taggctttca agcttgcctt 1501 tgtcttcccc tttgagggtg acagtttcgc caccctcaag agttacttta cacgtaatca 1561 atttacgcga cttcgatttc gtaaattgtg tgttcaggga agcgcttacc ctttggctta 1621 cgagttacaa cttcgtacgc tttaccttct tcgaacttcg ccgctagacc tttgtgtaga 1681 taaaggtcga actgttcacc agttaccagc tcagtgataa cgcctttagt catatcaggc 1741 ttagcgtctt ctttagcgct tacgtgctca gcgaacgctt taacgatacg gataaagtgc 1801 gatttagatt cgccgtcttt accgtagtct aaagagaaca tttgaagtga aacagttgct 1861 acgcgtttga aagttggttg agttgattta gccattttaa agctcctacc catatgggcg 1921 ttagtttggc gaaagtgccg tttggggcgt ttgccctctc agtgatttaa atatactaaa 1981 gttcgatttc gttgtaaacc cttttaagcg gtttttctaa tttttctttc attaaagcga 2041 ataccgcgtg aaatggagaa agtgggcgcg tcagatttcc acacaacttc cccgccttgt 2101 gccacttcga ccaattcgtc gtgagtaagc ctcgcgccct tactcgcggt ttttgtcttc 2161 ccgtccttta gaaccatcga atacaatttc ttacccgcta tagcgcaaaa gctcgcctcc 2221 ccttcgatat cccacgcgcc caacttcgtc gcgtcaatct ccaagtctcc caatccctca 2281 cagataatcg aatcagtatc gcaatagtaa ggcgtatcga ctgaacataa agcgcgcatc 2341 atgaacgctc gaacatatcc agtaatactc gcggcggtcg caacgttcaa aaacttcaat 2401 tcatcgtcgt acaaatcgcg ctcatataat aggttatccc cgatttcggt cgcgcacaca 2461 taatcagggt tgaagttctc gaactcttcc gccgtgtgta ccgactgcat agagtattgc 2521 ttgtatttag tcgggttagt ggcgtactta ccgtaaagac tattcataaa cagcttggcg 2581 aataaacgct tcgcttcatc cttttcaatg gtcgcctgtt ctttcatcac gtaaaaatgt 2641 tcgacgtaat tagcgaaaca aatcgaatct tcgaacgtaa gcacttcaat tatttctacg 2701 tcctctaata gtcccaattc ctccgccgcg taatattcgt gccctgtcac tttaaattcg 2761 tatatgttcc cgtcgtcggg gaatgacagt gaaccgtttt ctaacctaac aggaaagtga 2821 ccgttagact tacatttcag cgtgacgaaa atcttgacgt ctttttcatc aggcaagtgt 2881 cgcgactttg ttcgcttatt cccatacgca tggtcataca tcatcgcgta aggatacgcg 2941 gaattaatat ccacgtaata aaagtctttt tcaaacgtac cagttttaaa cgcttgaacc 3001 ctaccaccga aataatacgg acttattctt tcgaaatagt tcaaattggt tctaggcgtt 3061 tttaggtttg acatttggtt aaacactttt agcgctgtac tagccaaagt gatattgaaa 3121 ccgtattcag atacaaacgc agtaaccagt tcatgcaagt aaacgcagtc agattttaga 3181 taagcgcgta tagctttcca agtttccggt tcgtagcggg tttctttctc aaacagcgca 3241 taatcaattt catccttttg atacgctgcg agtggaatag gtaaaatatt atacgagtct 3301 ctaaaagtac atttaccgat ttgaaactcc gctattcttc ccgcgataat tttgacgctt 3361 ggcttaattt cacattcctg taaaacgaaa tgccagtcga acttcccgcc gttgtgagcg 3421 tatattgtcc attcttcatc gcgaacaaaa ttaataaagt cgattgtttt ttcgaactcg 3481 taatattgtt cattgatata taaaccccaa ataaacggct ttggtatacg tccatattta 3541 aacgggtcgg tctcacaatc cgccgcgcct atattttcca ataatcttct atttgcttta 3601 cgagtcattt taaattacct aaagaacgcg gattgaaaac cagtaataaa cgtttctgca 3661 ttctcgtatc tattaaccca atcttccatt tcatccgcta gtaattcagc ttcaaaactt 3721 tctgtagata aataatcgcc agtctttacc caaagctttt gtctatcagg tacgttatgt 3781 ttatctaata acccttgaac gtattcgcga gtatttaata aaaactctcc ttggttttct 3841 ataggaataa taaattgttt taatttgctt cttttattaa atgttttaac tattccattt 3901 ttaactctaa tgcttggcgc ttgcgaaccg ctataaggta caaacgctac tttccaacgt 3961 gggagagttt taaatacttg gtgataagta ttttgaactg tttttaaatt cgctttattt 4021 gaagaacgaa atatttttat attcattccc tgatataatt ctttgaattg attagtatat 4081 ttagtaatta atcgtttatc gccaatactt aactttttac gtaaatctat gttaaaatta 4141 acgtacggct ttattatttt aaataattct ttatattctt tttcagtcat tggagtaacc 4201 ttaattatga acacatcaga tatcgtagca gatgtagaaa acagcttttt agacaattta 4261 gacactttac cagaaagcgg tacaaatggc aaatttgaaa gaacgtcaga aatcggcaat 4321 ttctcagaaa gcgtggaaac agtagaaacg gttgaaactg tcgaagatag cgcaccctca 4381 gaaatcccgc gccacctgtt atgggacgaa tcgggcaatg aacgcgccga cgccgacggt 4441 aacactttcg acctttcttt acactcagta gacgacaacg gacacccgaa actaaccgcc 4501 aagggtaagc tacgtaaaaa gcgcgggcgc aagggcgcgt ctacactttc gtctagtacc 4561 gcatcgaaca aggtcgctca ggtcgctatc tctgaaaagg cgaaatttca agcggtcggc 4621 gcgggcgcgg cgaatgctct tatttgcttg ggtttagcta taggcggcga agacttcgca 4681 ccaatccgaa acgcagatat ggacgagaaa agtaatttag agaaagcgtt cgcggattgg 4741 gcggaaactc aagaagtcga agacatcccg ccaaacctag cgctaaccat cgtcgtaggc 4801 tcttaccttt tccctcgctt ggtgatgcct cgccaagtaa ctaagtttca gatgcttaag 4861 caatggctta aattgaagtt cttcaaaaag ccgacggcgg cgaaagtatt cgaccctaac 4921 aaggaggaaa attaatggct catagtgcaa tagtcggaat gactgaaagc ggcaaaagtt 4981 ccctagcgcg taaaatggct acggcgattc aaaaagctgg aatagctgta atagtattcg 5041 acccgctagg cgaccccgca tggagcgaat gcctagaccc caataactca tttattacag 5101 ataacgaaaa agaattttta aattattatt ggtcttctaa aaattgcgcg gtatttttcg 5161 atgaagcggg agactacgcg acgaaccata ataaagaaat gattcgaacc gctaccaaag 5221 gtagacattg gggacattct aattattata tcgcgcaacg cggtaattta ttagcgagaa 5281 ctattcgcga ccaatgctct aaattatata tgttcacatc gtctaaagat gatgctaaaa 5341 tatattcttt agaatttaat gataacgaat tattaaatgt acccgattta tcgcaaggcg 5401 aatattattc atgcgggcga tttaataaag ctgttaaaca tattttattc aaagggaaat 5461 caaaatgatt ccaatcggtt tacgttttat tgcttattta ggggcggctc tatttttaga 5521 atcgcaaatt aaaaccaagg agaaaaatca cgatgctgat gaaattgaaa ctccgccgca 5581 ttcgccagaa tcagaagtta aaaaagattc aggtagcgaa agcggaagcg gaagccaaag 5641 cgaaagcgga agcggaagcg aagacccaag cggaagccaa agcgaaagcg gaagcggaag 5701 cgaagaccca agcggaaacc aaagcgaaag cggaagcgga agcgaaacag gcagctaatg 5761 aagcgctaaa aaacgctcag aaatctgcga ccgtaggcga gtaatatgac tttaaataca 5821 aaagcggcaa aaatggcggt ttggacggct ttagcctctt atgtttttga agcatatatc 5881 cgccctaaac tgtaggagcg ttacctcatg gatattcgca acgttatcat gattgcaggt 5941 gtttcagcac ttacgacgct tgtaatcaat aactcaccat tgaagcgcta tttccgttaa 6001 tagcgcttta aaacactaac aaactttgga gtaattccca tgcctaaaaa gtatatgcct 6061 tacgcgatta acgcaatcgt agcagctaca accgcttacc ttgtaacgtc ggcagttgct 6121 aaacaaaagc aagtggtttg gtacaatccg acaacttggc tataattcgc agttttcgcg 6181 ggttaaatcc ttttcatcaa tttagacaga gagaatactt tatgtcattt cgttcaaata 6241 aagtcttacc taacttcaac ggtgtaggcg caggtcaaac cgctacgcta gacgtaccaa 6301 ttggctcgac ttaccatcaa atcgctttcg aaatcgcggg cgtatcacct caagagctta 6361 agaactttaa agttctttta aatggtatcc cgcatatcga actgccgtca atgtcaactc 6421 tagctgaaat gaacgagttt gacggaatcg gcggcgctat gggcgtactg attctagatt 6481 tcgaacgttt cggtcttcta acccgtcaag cgcgtgaact gaccgcaatc ggtacgggta 6541 tgggcgacgc tgaccctaac caagttcgta cattccagat tcagatggat atcgaccccg 6601 ctgcgactaa cccgtcaatc cgtgcgaccg cgacaacttc gccagcttca atgtctgggc 6661 ttatccgtaa aattcgtttg ttcaacaaaa cgccgacagg ctcaggccta ttcgaaatct 6721 ctgatttacc taaaggtgat atgattaaca aagttttctt tgttgctcaa gaagacacga 6781 aaattgataa gctcactcta gagcttgaca actttgtgaa gtttgaacgt acagcgggct 6841 tgaacgatgt tcttcaaatg aacgcgggtg ttcgtgtacc taacccgaaa atgtacgtac 6901 tcgaccctac tgaaatgggc tacggcgcgg aagttatcga cacggtttat ccggcgggac 6961 atccacgcga aggccaaaac ctttcagacg ttcgcttcaa attgaacatg tcaggcggtc 7021 aaggtcagct tgttatcgtt gaatatcttg gtgcggtcga acgctaacgc gacgctgtaa 7081 catttcaaat taaaccaaag ggcgggcgca gtgcccgccc tttttacata acggagtaat 7141 ccaaatgaat gtagattcta atctttttga aaactggttt ggcacaacaa gcgcgcaagg 7201 tcagcaaacc tcgctttggg ataaactggt taacgcgggc ggtcaagtaa tcgacaccct 7261 acctgatatc gtagacggcg tggtcgattc aaagttaaac gcggaaatca agaacaaaac 7321 cgaaacgact gaaaatatca catcgactaa cccgagtgtg tcaggttcag caaatcagct 7381 aacattcttg aaagacccta aagtcatgta tggcggtcta gctatcggcg gtattatcgc 7441 ggcggctatt cttatcaagg cgcttaaata agggagggcg cacaatgcct tttttattac 7501 ctattgcagt tttagcgggt acgggtgtct ttgcttggaa agcgggcgac gccgtaaaca 7561 acgtttcaga taagctagtc agtgccgcca ttatcggtgg cgcggcttac ctaatttaca 7621 aagggagtaa ataactatgc aagcagcagg cggcggcgga atgtcagcgg gttctagtgc 7681 gggcgcggtt ggtggcgcat ctggctctag tatcggtggc gtaaatatcg acctaagcgg 7741 tcgtggtgat atgaatgtta catatctagt tggcggcgct ttagcggttg gtgcgctttt 7801 cattttctta actaagcgaa agtagggtaa atcatgaata agttcgagcc agtcccaatt 7861 gatgaaatac ttgaagccat gccaaagagt atccataggc atacggattt agtaaagcgt 7921 caactcaaag cacagcacca gtacggttat aaattccgtg gtttgtacat aattttcgaa 7981 ccgtgggacg atactctaat ggttcaatgt gtcgagggaa cgggattgga tgaggacttc 8041 gtaaaagccc tttataagtg cgctataaat aacggcttcg aatacatcca atttgtaacg 8101 ccacataaag ggctacataa aatggttaaa tccctcaaac cggaacgaat agaatttcgt 8161 tatatttgta agctcgacga ggtggaatta tgagtttttc acgaggcggt tcaagcgaat 8221 cagccaccac aaacgaacaa aaccctatca ccgcgactga taatgctatg gtcgccagtg 8281 agggtggaac aattacagtc actgacggcg gcgcgttcaa cgtagtaact gacgcaatcg 8341 acgcaatcgg tgacgtagtt ctaagcgctg aatcacttgc taagaacgcg atgagttcaa 8401 cggagtcaat cgcgtctgac tcaatcaacg cgggacttga ctatcattcc caaacaactg 8461 gctttcttga gggaacgctt gacaaggttt tcaactttgt cggggatacc gtccagaaat 8521 cacaagatac aatttctgga aatactcaag ccctttttaa ttctgcaaaa tcggatagcc 8581 taaaccgctc cgaagatatg caacaaacga ttcgaacgct tggtatgtac gcgactattg 8641 gtttagtcgg ttatactgct tttcgattcc taaagaaata aggagttaat atgtctctta 8701 ttcgtgacta tgatttgaac atcgaatcaa acgctacaaa atcttttgcc accactggca 8761 ctatgattgc agttcgtcgc ggtgacgacc ctattcgcgt tcgcgtaaaa gcttacacca 8821 aagagggcgg aacgcttgta actgatagcg tcctatcagc gggtgaaaaa gttcgaacgg 8881 gtgaagaatt taacttcatc gaaatcacta actatgagct aaccaagcga cttgttcgag 8941 ttacattggg cgacggcgat tttcaatccg acgttgtaga gggcgaagtc gtcgttcgaa 9001 gtgataccga taacccgctt caagttattt caaagcgcgc accgcgtgat gtgttgtcag 9061 ctttcatcac gcaccaaaag ggaacggata cccctgtaac cagtccagat ggtacgatta 9121 aatttagtgc gcagaatctg acaggcgggg atatctatat tttcattggt aacgcgtata 9181 tttatctagc gcctacgcaa ttgttttcaa ctgatattgc tcaagatact cgcatcaaag 9241 tggcgggaat ttccgagggc ggtcagattt ctatgttctg ggttatcgag gggtaatacc 9301 ttatgactct aaaaccttta ttaatagtga taccgctatt aggagtttat tacatgagta 9361 aacgctcacc cgctttacac gcttcgcgag gttatcgaaa caataacccg cttaacattc 9421 gtataggttc ttcaaagtgg aaaggggaaa ttccaaccga taagaacaca gacggtaaat 9481 ttgaacaatt ttataagcgc tccgacggat accgcgcggc ggcgaaattg attttgaatt 9541 ataaccgcct atacaatatt ttcactgttc aacgtattat cgagcgatgg gctcccgcta 9601 tcgggaacgc ccccgacggg acgaaataca aaaaccatac gcatcaatac attgactttg 9661 tttgtcgcaa aacaggattc gacccgaaca cactaattaa cgggtcgaac gtaggggagt 9721 tggtttgggc tatggctcag ttcgagaaca tgccgcctca taccgattca cttgactttg 9781 ttaaaaactc cgttagcgag ttcaaagata tcaactacta ggaggcttta tgtttctttc 9841 agcagttcca caaaagaaag ttaaccccgc cagaattcag caaactttgt cggcctccaa 9901 catggtaagc gttacaatcc ccgctaaaac ttggcgggtg atgagcgccg atgtgccgga 9961 taacgttgct acgcttgtat ttgtcattac gcgtgattac tcttcagcac ccgctgacac 10021 tggtgtcacc attgaaagag ttagcgacgg acacactgtt tacgaggcta ccgtatccga 10081 ctcgccgaac acctccgaat atgcaacgct acaagtttcg cgtccgtatg agggttgggg 10141 ttctctcaag atatataata atactgctaa cgcgggacgt cttataatca acgcgtggac 10201 gaataccgac ctatacgcat aaggagtcat tatgcctaac gtacatccta aaaagttcaa 10261 tgtaatagcg ggtattctcg cgggcgctct agtcgggttc ggcggtggtt cggttaatct 10321 gaattacaac gcttccccta atgaattaca actgctagac tgtttaacaa caattgacaa 10381 tatgattaag tcgcatgacg ataatttggg aatggtagtc cagtctctta tggaatcaaa 10441 gattcaatca taacgtttca ctgttttcgg gtgtttggcg ctgctcctgc ctttcacccg 10501 ccttttctct tggttcacac ttcccctttc ttgtcaaatt taaatctcat aaatgtgatg 10561 tatatccgca aatatgcg //