LOCUS MG592663 10580 bp DNA linear VRL 24-JAN-2018 DEFINITION Vibrio phage 2.092.O._10N.286.52.B7, partial genome. ACCESSION MG592663 VERSION MG592663.1 DBLINK BioProject: PRJNA328102 KEYWORDS . SOURCE Vibrio phage 2.092.O._10N.286.52.B7 ORGANISM Vibrio phage 2.092.O._10N.286.52.B7 Viruses; dsDNA viruses, no RNA stage; unclassified dsDNA viruses. REFERENCE 1 (bases 1 to 10580) AUTHORS Kauffman,K.M., Hussain,F.A., Yang,J., Arevalo,P., Brown,J.M., Chang,W.K., VanInsberghe,D., Elsherbini,J., Cutler,M.B., Kelly,L. and Polz,M.F. TITLE A major lineage of nontailed dsDNA viruses as unrecognized killers of marine bacteria JOURNAL Unpublished REFERENCE 2 (bases 1 to 10580) AUTHORS Kauffman,K.M., Hussain,F.A., Yang,J., Arevalo,P., Brown,J.M., Chang,W.K., VanInsberghe,D., Elsherbini,J., Cutler,M.B., Kelly,L. and Polz,M.F. TITLE Direct Submission JOURNAL Submitted (29-NOV-2017) Department of Civil and Environmental Engineering, Massachusetts Institute of Technology, 15 Vassar Street, Bldg. 48-417, Cambridge, MA 02139, USA FEATURES Location/Qualifiers source 1..10580 /organism="Vibrio phage 2.092.O._10N.286.52.B7" /mol_type="genomic DNA" /isolation_source="iron chloride precipitation of 0.2um-filtered seawater, resuspended in oxalate solution" /host="Vibrio lentus" /db_xref="taxon:1881378" /lab_host="10N.286.52.B7" /country="USA: Massachusetts, Nahant, Canoe Cove" /lat_lon="42.419 N 70.906 W" /collection_date="2010-10-13" /collected_by="Kathryn Kauffman" gene complement(130..321) /locus_tag="NVP2092O_01" CDS complement(130..321) /locus_tag="NVP2092O_01" /note="NVP2092O_01" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AUS02061.1" /translation="MIISINNWSGGKVKIFNRTSGKVIRLEDGESETVETAAITSLII LRCILANSHLILFDIVNTD" gene complement(323..529) /locus_tag="NVP2092O_02" CDS complement(323..529) /locus_tag="NVP2092O_02" /note="NVP2092O_02" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AUS02062.1" /translation="MARYVKSKLTGVLYLITNYMEDDADDIIEYMHDQWGDHYELDPV PLPHEGGVKLYWCQVRQSLIHLKE" gene complement(529..747) /locus_tag="NVP2092O_03" CDS complement(529..747) /locus_tag="NVP2092O_03" /note="NVP2092O_03" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AUS02063.1" /translation="MARMFQIRNHGEESVTMIHAVADTASFKAQMVELEAGAFVVMPE PELENYALPLSLGAKVNLVTVTELERVQ" gene complement(750..1367) /locus_tag="NVP2092O_04" CDS complement(750..1367) /locus_tag="NVP2092O_04" /note="NVP2092O_04" /codon_start=1 /transl_table=11 /product="TMhelix containing protein" /protein_id="AUS02064.1" /translation="MVMTIYFCLGLCILYAAHRLAAAGYHGIALAFVLVLCVASHVGA VETEPESLDTSGTWLDFSEFGLPTVLEPLEGKTKIFMGDAYSYHFDRSYDYNEVHRTY GFEYKITENYAVAFTNFTNSYNKQSNALSIVYTWRDYRLDNNIGLTVGAQGGIADGYE KAKYNSNGFAPIVAPFVDITVYESIGARISVWNLYVANIAFYLEF" gene complement(1564..1896) /locus_tag="NVP2092O_05" CDS complement(1564..1896) /locus_tag="NVP2092O_05" /note="NVP2092O_05" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AUS02065.1" /translation="MAKSTQPTFKRVATVSLQMFSLDYGKDGESKSHFIRIVKAFAEH VSAKEDAKPDMTKGVITELVTGEQFDLYLHKGLAAKFEEGKAYEVVTRKPKGKRFPEH TIYEIEVA" gene complement(2005..3609) /locus_tag="NVP2092O_06" CDS complement(2005..3609) /locus_tag="NVP2092O_06" /note="NVP2092O_06; aclame_best_match:protein:vir:2124; tara_best_match:OM-RGC.v1.001450554; cvp_best_match:RF_PEP_9626351; InterPro:IPR004868; pfam:PF03175|pog:POG0334" /codon_start=1 /transl_table=11 /product="DNA polymerase type B" /protein_id="AUS02066.1" /translation="MTRKANRRLLENIGAADCETDPFKYGRIPKPFIWGLYINEQYYE FEKTIDFINFVRDEEWTIYAHNGGKFDWHFVLQECEIKPSVKIIAGRIAEFQIGKCTF RDSYNILPIPLAAYQKDEIDYALFEKETRYEPETWKAIRAYLKSDCVYLHELVTAFVS EYGFNITLASTALKVFNQMSNLKTPRTNLNYFERISPYYFGGRVQAFKTGTFEKDFYY VDINSAYPYAMMYDHAYGNKRTKSRHLPDEKDVKIFVTLKCKSNGHFPVRLENGSLSF PDDGNIYEFKVTGHEYYAAEELGLLEDVEIIEVLTFEDSICFANYVEHFYVMKEQATI EKDEAKRLFAKLFMNSLYGKYATNPTKYKQYSMQSVHTAEEFENFNPDYVCATEIGDN LLYERDLYDDELKFLNVATAASITGYVRAFMMRALCSVDTPYYCDTDSIICEGLGDLE IDATKLGAWDIEGEASFCAIAGKKLYSMVLKDGKTKTASKGARLTHDELVEVAQGGEV VWKSDAPTFSISRGIRFNERKIRKTA" gene complement(3616..4191) /locus_tag="NVP2092O_07" CDS complement(3616..4191) /locus_tag="NVP2092O_07" /note="NVP2092O_07; Putative terminal protein" /codon_start=1 /transl_table=11 /product="putative terminal protein" /protein_id="AUS02067.1" /translation="MTEKEYKELFKIIKPYVNFNIDLRKKLSIGDKRLITKYTNQFKE LYQGMNIKIFRSSNKANLKTVQNTYHQVFKTLPRWKVAFVPYSGSQAPSIRVKNGIVK TFNKRSKLKQFIIPIENQGEFLLNTREYVQGLLDKHNVPDRQKLWVKTGDYLSTESFE AELLADEMEDWVNRYENAETFITGFQSAFFR" gene 4208..4936 /locus_tag="NVP2092O_08" CDS 4208..4936 /locus_tag="NVP2092O_08" /note="NVP2092O_08" /codon_start=1 /transl_table=11 /product="coil containing protein" /protein_id="AUS02068.1" /translation="MNTSDIVADVENSFLDNLDTLPESGTNGKFERTSEIGNFSESAE TVETVETVEDSAPSEIPRHLLWDESGNERADADGNTFDLSLHSVDDNGHPKLTAKGKL RKKRGRKGASTLSSSTASNKVAQVAISEKAKFQAVGAGAANALICLGLAIGGEDFAPI RNADMDEKSNLEKAFADWAETQEVEDIPPNLALTIVVGSYLFPRLVMPRQVTKFQMLK QWLKLKFFKKPTAAKVFDPNKEEN" gene 4936..5469 /locus_tag="NVP2092O_09" CDS 4936..5469 /locus_tag="NVP2092O_09" /note="NVP2092O_09; tara_best_match:OM-RGC.v1.021972741; InterPro:IPR002789; Putative packaging ATPase" /codon_start=1 /transl_table=11 /product="putative packaging ATPase" /protein_id="AUS02069.1" /translation="MAHSAIVGMTESGKSSLARKMATAIQKAGIAVIVFDPLGDPAWS ECLDPNNSFITDNEKEFLNYYWSSKNCAVFFDEAGDYATNHNKEMIRTATKGRHWGHS NYYIAQRGNLLARTIRDQCSKLYMFTSSKDDAKIYSLEFNDNELLNVPDLSQGEYYSC GRFNKAVKHILFKGKSK" gene 5554..5805 /locus_tag="NVP2092O_10" CDS 5554..5805 /locus_tag="NVP2092O_10" /note="NVP2092O_10; aclame_best_match:protein:proph:183884; tara_best_match:OM-RGC.v1.017471225; cvp_best_match:RF_PEP_370703036; kegg:none|pfam:PF08017|cog:COG1652|pog:POG0623" /codon_start=1 /transl_table=11 /product="fibrinogen binding protein" /protein_id="AUS02070.1" /translation="MLMKLKLRRIRQNQKLKKIQVAKAEAEAKAKAEAEAKTQAEAKA KAEAEAKTQAEAKAKAEAEAKQAANEALKNAQKSATVGE" ncRNA 5638..5658 /ncRNA_class="scRNA" /locus_tag="NVP2092O_10" /product="NVP2092O_CRISPR_spacer_1" /note="CRISPR spacer 1" ncRNA 5680..5700 /ncRNA_class="scRNA" /locus_tag="NVP2092O_10" /product="NVP2092O_CRISPR_spacer_2" /note="CRISPR spacer 2" gene 6041..6166 /locus_tag="NVP2092O_11" CDS 6041..6166 /locus_tag="NVP2092O_11" /note="NVP2092O_11" /codon_start=1 /transl_table=11 /product="TMhelix containing protein" /protein_id="AUS02071.1" /translation="MPKKYMPYAINAIVAATTAYLVTSAVAKQKQVVWYNPTTWL" gene 6224..7069 /locus_tag="NVP2092O_12" CDS 6224..7069 /locus_tag="NVP2092O_12" /note="NVP2092O_12; tara_best_match:OM-RGC.v1.009973837; Double jelly roll capsid protein" /codon_start=1 /transl_table=11 /product="coil containing protein" /protein_id="AUS02072.1" /translation="MSFRSNKVLPNFNGVGAGQTATLDVPIGSTYHQIAFEIAGVSPQ ELKNFKVLLNGIPHIELPSMSTLAEMNEFDGIGGAMGVLILDFERFGLLTRQARELTA IGTGMGDADPNQVRTFQIQMDIDPAATNPSIRATATTSPASMSGLIRKIRLFNKTPTG SGLFEISDLPKGDMINKVFFVAQEDTKIDKLTLELDNFVKFERTAGLNDVLQMNAGVR VPNPKMYVLDPTEMGYGAEVIDTVYPAGHPREGQNLSDVRFKLNMSGGQGQLVIVEYL GAVER" gene 7147..7473 /locus_tag="NVP2092O_13" CDS 7147..7473 /locus_tag="NVP2092O_13" /note="NVP2092O_13" /codon_start=1 /transl_table=11 /product="TMhelix containing protein" /protein_id="AUS02073.1" /translation="MNVDSNLFENWFDTTSAQGQQTSLWDKLVNAGGQVIDTLPDIVD GVVDSKLNAEIKNKTETTENITSTNPSVSGSANQLTFLKDPKVMYGGLAIGGIIAAAI LIKALK" gene 7487..7636 /locus_tag="NVP2092O_14" CDS 7487..7636 /locus_tag="NVP2092O_14" /note="NVP2092O_14" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AUS02074.1" /translation="MPFLLPIAVLAGTGVFAWKAGDAVNNVSDKLVSAAIIGGAAYLI YKGSK" gene 7639..7827 /locus_tag="NVP2092O_15" CDS 7639..7827 /locus_tag="NVP2092O_15" /note="NVP2092O_15" /codon_start=1 /transl_table=11 /product="TMhelix containing protein" /protein_id="AUS02075.1" /translation="MQAAGGGGMSAGSSAGAVGGASGSSIGGVNIDLSGRGDMNVTYL VGGALAVGALFIFLTKRK" gene 7836..8195 /locus_tag="NVP2092O_16" CDS 7836..8195 /locus_tag="NVP2092O_16" /note="NVP2092O_16" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AUS02076.1" /translation="MNKFEPVPIDEILEAMPKSIHRHTDLVKRQLKAQHQYGYKFRGL YIIFEPWDDTLMVQCVEGTGLDEDFVKALYKCAINNGFEYIQFVTPHKGLHKMVKSLK PERIEFRYICKLDEVEL" gene 8192..8683 /locus_tag="NVP2092O_17" CDS 8192..8683 /locus_tag="NVP2092O_17" /note="NVP2092O_17" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AUS02077.1" /translation="MSFSRGGSSESATTNEQNPITATDNAMVASEGGTITVTDGGAFN VVTDAIDAIGDVVLSAESLAKNAMSSTESIASDSINAGLDYHSQTTGFLEGTLDKVFN FVGDTVQKSQDTISGNTQALFNSAKSDSLNRSEDMQQTIRTLGMYATIGLVGYTAFRF LKK" gene 8693..9298 /locus_tag="NVP2092O_18" CDS 8693..9298 /locus_tag="NVP2092O_18" /note="NVP2092O_18" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AUS02078.1" /translation="MSLIRDYDLNIESNATKSFATTGTMIAVRRGDDPIRVRVKAYTK EGGTLVTDSVLSAGEKVRTGEEFNFIEITNYELTKRLVRVTLGDGDFQSDVVEGEVVV RSDTDNPLQVISKRAPRDVLSAFITHQKGTDTPVTSPDGTIKFSAQNLTGGDIYIFIG NAYIYLAPTQLFSTDIAQDTRIKVAGISEGGQISMFWVIEG" gene 9356..9823 /locus_tag="NVP2092O_19" CDS 9356..9823 /locus_tag="NVP2092O_19" /note="NVP2092O_19; tara_best_match:OM-RGC.v1.019721584" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AUS02079.1" /translation="MSKRSPALHASRGYRNNNPLNIRIGSSKWKGEIPTDKNTDGKFE QFYKRSDGYRAAAKLILNYNRLYNIFTVQRIIERWAPAIGNAPDGTKYKNHTHQYIDF VCRKTGFDPNTLINGSNVGELVWAMAQFENMPPHTDSLDFVKNSVSEFKDINY" gene 9832..10224 /locus_tag="NVP2092O_20" CDS 9832..10224 /locus_tag="NVP2092O_20" /note="NVP2092O_20" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AUS02080.1" /translation="MFLSAVPQKKVNPARIQQTLSASNMVSVTIPAKTWRVMSADVPD NVATLVFVITRDYSSAPADTGVTIERVSDGHTVYEATVSDSPNTSEYATLQVSRPYEG WGSLKIYNNTANAGRLIINAWTNTDLYA" gene 10234..10455 /locus_tag="NVP2092O_21" CDS 10234..10455 /locus_tag="NVP2092O_21" /note="NVP2092O_21" /codon_start=1 /transl_table=11 /product="TMhelix containing protein" /protein_id="AUS02081.1" /translation="MPNVHPKKFNVIAGILAGALVGFGGGSVNLNYNASPNELQLLDC LTTIDNMIKSHDDNLGMVVQSLMESKIQS" ORIGIN 1 cgcatatttg cggatataca tcacatttat gagatttaaa tttgacaaga aaggggaagt 61 gtgtaagagg cgcgccgcac tcaggcgaag ccgtgaaagg agtgaaacga aaaaaagccc 121 ccgtaggggc taatcagtgt ttacaatatc gaaaagtatt aaatgcgaat tggcaagtat 181 acagcgtaaa ataatcaagg atgtgatagc ggcagtttct accgtttcgc tttccccgtc 241 ctctaatcta atgaccttac ccgacgttcg attaaatatt ttaacctttc cgcccgacca 301 attattgatt gatataatca tgctattcct ttaggtgaat caaactttgt cgcacttggc 361 accaatacaa tttaaccccg ccttcatgcg gaagcggtac ggggtcaagt tcgtaatggt 421 cgccccattg gtcatgcata tactcgatga tatcgtctgc gtcgtcttcc atgtaattag 481 tgatgagata caaaacgccc gttaattttg attttacata tctagccatt actgaaccct 541 ctccaattct gtaacagtaa ctaagtttac tttagcaccc aacgatagcg gtaaagcgta 601 gttctccaac tctggctctg gcatcaccac gaacgcgcca gcttccaact caaccatttg 661 agctttaaac gacgcagtgt ccgcaactgc gtgaatcatc gtaactgatt cctcgccgtg 721 gtttcgaatt tgaaacattc tagccatttt tagaactcca agtaaaaagc gatgttagca 781 acgtatagat tccaaacact gattcgcgca ccaatggatt catatacagt gatatcaaca 841 aaaggagcaa caataggagc gaaaccgttc gagttatatt tagctttttc gtaaccatcc 901 gcaattccgc cttgcgcacc aactgtaagc ccaatattgt tgtcgagcct gtaatcgcgc 961 caagtataaa cgattgagag cgcgttagat tgcttgttgt aagagtttgt gaaatttgtg 1021 aaagcgactg cgtaattttc tgtgattttg tattcgaatc cataagttct gtgaacctcg 1081 ttataatcat atgaacggtc gaagtgatac gagtaagcat cacccataaa aatctttgtc 1141 tttccctcaa gcggctctag aactgtagga agtccgaact cgctaaagtc caaccaagta 1201 cccgaagtat ctaaactttc cggctcagtt tccaccgccc cgacatggga agcgacacaa 1261 agaactaaaa cgaacgccaa agctattcca tgataccccg ccgccgctag tcgatgcgcc 1321 gcgtaaagaa tacaaagtcc caaacaaaaa taaatagtca taaccattgt aaagctccta 1381 atgcaaaagg gcgtatcaga tttgccgctg atacgcccta agttctaatt ttttaatttc 1441 caattgattg cttgaatttc tgactgtggt aacaaggaaa ctaggctttc aagcttgcct 1501 ttgtcttccc ctttgagggt gacagtttcg ccaccctcaa gagttacttt acacgtaatc 1561 aatttacgcg acttcgattt cgtaaattgt gtgttcaggg aagcgcttac cctttggctt 1621 acgagttaca acttcgtacg ctttaccttc ttcgaacttc gccgctagac ctttgtgtag 1681 ataaaggtcg aactgttcac cagttaccag ctcagtgata acgcctttag tcatatcagg 1741 cttagcgtct tctttagcgc ttacgtgctc agcgaacgct ttaacgatac ggataaagtg 1801 cgatttagat tcgccgtctt taccgtagtc taaagagaac atttgaagtg aaacagttgc 1861 tacgcgtttg aaagttggtt gagttgattt agccatttta aagctcctac ccatatgggc 1921 gttagtttgg cgaaagtgcc gtttggggcg tttgccctct cagtgattta aatatactaa 1981 agttcgattt cgttgtaaac ccttttaagc ggtttttcta atttttcttt cattaaagcg 2041 aataccgcgt gaaatggaga aagtgggcgc gtcagatttc cacacaactt ccccgccttg 2101 tgccacttcg accaattcgt cgtgagtaag cctcgcgccc ttactcgcgg tttttgtctt 2161 cccgtccttt agaaccatcg aatacaattt cttacccgct atagcgcaaa agctcgcctc 2221 cccttcgata tcccacgcgc ccaacttcgt cgcgtcaatc tccaagtctc ccaatccctc 2281 acagataatc gaatcagtat cgcaatagta aggcgtatcg actgaacata aagcgcgcat 2341 catgaacgct cgaacatatc cagtaatact cgcggcggtc gcaacgttca aaaacttcaa 2401 ttcatcgtcg tacaaatcgc gctcatataa taggttatcc ccgatttcgg tcgcgcacac 2461 ataatcaggg ttgaagttct cgaactcttc cgccgtgtgt accgactgca tagagtattg 2521 cttgtattta gtcgggttag tggcgtactt accgtaaaga ctattcataa acagcttggc 2581 gaataaacgc ttcgcttcat ccttttcaat ggtcgcctgt tctttcatca cgtaaaaatg 2641 ttcgacgtaa ttagcgaaac aaatcgaatc ttcgaacgta agcacttcaa ttatttctac 2701 gtcctctaat agtcccaatt cctccgccgc gtaatattcg tgccctgtca ctttaaattc 2761 gtatatgttc ccgtcgtcgg ggaatgacag tgaaccgttt tctaacctaa caggaaagtg 2821 accgttagac ttacatttca gcgtgacgaa aatcttgacg tctttttcat caggcaagtg 2881 tcgcgacttt gttcgcttat tcccatacgc atggtcatac atcatcgcgt aaggatacgc 2941 ggaattaata tccacgtaat aaaagtcttt ttcaaacgta ccagttttaa acgcttgaac 3001 cctaccaccg aaataatacg gacttattct ttcgaaatag ttcaaattgg ttctaggcgt 3061 ttttaggttt gacatttggt taaacacttt tagcgctgta ctagccaaag tgatattgaa 3121 accgtattca gatacaaacg cagtaaccag ttcatgcaag taaacgcagt cagattttag 3181 ataagcgcgt atagctttcc aagtttccgg ttcgtagcgg gtttctttct caaacagcgc 3241 ataatcaatt tcatcctttt gatacgctgc gagtggaata ggtaaaatat tatacgagtc 3301 tctaaaagta catttaccga tttgaaactc cgctattctt cccgcgataa ttttgacgct 3361 tggcttaatt tcacattcct gtaaaacgaa atgccagtcg aacttcccgc cgttgtgagc 3421 gtatattgtc cattcttcat cgcgaacaaa attaataaag tcgattgttt tttcgaactc 3481 gtaatattgt tcattgatat ataaacccca aataaacggc tttggtatac gtccatattt 3541 aaacgggtcg gtctcacaat ccgccgcgcc tatattttcc aataatcttc tatttgcttt 3601 acgagtcatt ttaaattacc taaagaacgc ggattgaaaa ccagtaataa acgtttctgc 3661 attctcgtat ctattaaccc aatcttccat ttcatccgct agtaattcag cttcaaaact 3721 ttctgtagat aaataatcgc cagtctttac ccaaagcttt tgtctatcag gtacgttatg 3781 tttatctaat aacccttgaa cgtattcgcg agtatttaat aaaaactctc cttggttttc 3841 tataggaata ataaattgtt ttaatttgct tcttttatta aatgttttaa ctattccatt 3901 tttaactcta atgcttggcg cttgcgaacc gctataaggt acaaacgcta ctttccatcg 3961 tgggagagtt ttaaatactt ggtgataagt attttgaact gtttttaaat tcgctttatt 4021 tgaagaacga aatattttta tattcattcc ctgatataat tctttgaatt gattagtata 4081 tttagtaatt aatcgtttat cgccaatact taacttttta cgtaaatcta tgttaaaatt 4141 aacgtacggc tttattattt taaataattc tttatattct ttttcagtca ttggagtaac 4201 cttaattatg aacacatcag atatcgtagc agatgtagaa aacagctttt tagacaattt 4261 agacacttta ccagaaagcg gtacaaatgg caaatttgaa agaacgtcag aaatcggcaa 4321 tttctcagaa agcgcggaaa cagtagaaac ggttgaaact gtcgaagata gcgcaccctc 4381 agaaatcccg cgccacctgt tatgggacga atcgggcaat gaacgcgccg acgccgacgg 4441 taacactttc gacctttctt tacactcagt agacgacaac ggacacccga aactaaccgc 4501 caagggtaag ctacgtaaaa agcgtgggcg caagggcgcg tctacacttt cgtctagtac 4561 cgcatcgaac aaggtcgctc aggtcgctat ctctgaaaag gcgaaatttc aagcggtcgg 4621 cgcgggcgcg gcgaatgctc ttatttgctt gggtttagct ataggcggcg aagacttcgc 4681 accaatccga aacgcagata tggacgagaa aagtaattta gagaaagcgt tcgcggattg 4741 ggcggaaact caagaagtcg aagacatccc gccaaaccta gcgctaacca tcgtcgtagg 4801 ctcttacctt ttccctcgct tggtgatgcc tcgccaagta actaagtttc agatgcttaa 4861 gcaatggctt aaattgaagt tcttcaaaaa gccgacggcg gcgaaagtat tcgaccctaa 4921 caaggaggaa aattaatggc tcatagtgca atagtcggaa tgactgaaag cggcaaaagt 4981 tccctagcgc gtaaaatggc tacggcgatt caaaaagctg gaatagctgt aatagtattc 5041 gacccgctag gcgaccccgc atggagcgaa tgcctagacc ccaataactc atttattaca 5101 gataacgaaa aagaattttt aaattattat tggtcttcta aaaattgcgc ggtatttttc 5161 gatgaagcgg gagactacgc gacgaaccat aataaagaaa tgattcgaac cgctaccaaa 5221 ggtagacatt ggggacattc taattattat atcgcgcaac gcggtaattt attagcgaga 5281 actattcgcg accaatgctc taaattatat atgttcacat cgtctaaaga tgatgctaaa 5341 atatattctt tagaatttaa tgataacgaa ttattaaatg tacccgattt atcgcaaggc 5401 gaatattatt catgcgggcg atttaataaa gctgttaaac atattttatt caaagggaaa 5461 tcaaaatgat tccaatcggt ttacgtttta ttgcttattt aggggcggct ctatttttag 5521 aatcgcaaat taaaaccaag gagaaaaatc acgatgctga tgaaattgaa actccgccgc 5581 attcgccaga atcagaagtt aaaaaagatt caggtagcga aagcggaagc ggaagccaaa 5641 gcgaaagcgg aagcggaagc gaagacccaa gcggaagcca aagcgaaagc ggaagcggaa 5701 gcgaagaccc aagcggaagc caaagcgaaa gcggaagcgg aagcgaaaca ggcagctaat 5761 gaagcgctaa aaaacgctca gaaatctgcg accgtaggcg agtaatatga ctttaaatac 5821 aaaagcggca aaaatggcgg tttggacggc tttagcctct tatgtttttg aagcatatat 5881 ccgccctaaa ctgtaggagc gttacctcat ggatattcgc aacgttatca tgattgcagg 5941 tgtttcagca cttacgacgc ttgtaatcaa taactcacca ttgaagcgct atttccgtta 6001 atagcgcttt aaaacactaa caaactttgg agtaattccc atgcctaaaa agtatatgcc 6061 ttacgcgatt aacgcaatcg tagcagctac aaccgcttac cttgtaacgt cggcagttgc 6121 taaacaaaag caagtggttt ggtacaatcc gacaacttgg ctataattcg cagttttcgc 6181 gggttaaatc cttttcatca atttagacag agagaatact tttatgtcat ttcgttcaaa 6241 taaagtctta cctaacttca acggtgtagg cgcaggtcaa accgctacgc tagacgtacc 6301 aattggctcg acttaccatc aaatcgcttt cgaaatcgcg ggcgtatcac ctcaagagct 6361 taagaacttt aaagttcttt taaatggtat cccgcatatc gaactgccgt caatgtcaac 6421 tctagctgaa atgaacgagt ttgacggaat cggcggcgct atgggcgtac tgattctaga 6481 tttcgaacgt ttcggtcttc taacccgtca agcgcgtgaa ctgaccgcaa tcggtacggg 6541 tatgggcgac gctgacccta accaagttcg tacattccag attcagatgg atatcgaccc 6601 cgctgcgact aacccgtcaa tccgtgcgac cgcgacaact tcgccagctt caatgtctgg 6661 gcttatccgt aaaattcgtt tgttcaacaa aacgccgaca ggctcaggcc tattcgaaat 6721 ctctgattta cctaaaggtg atatgattaa caaagttttc tttgttgctc aagaagacac 6781 gaaaattgat aagctcactc tagagcttga caactttgtg aagtttgaac gtacagcggg 6841 cttgaacgat gttcttcaaa tgaacgcggg tgttcgtgta cctaacccga aaatgtacgt 6901 actcgaccct actgaaatgg gctacggcgc ggaagttatc gacacggttt atccggcggg 6961 acatccacgc gaaggccaaa acctttcaga cgttcgcttc aaattgaaca tgtcaggcgg 7021 tcaaggtcag cttgttatcg ttgaatatct tggtgcggtc gaacgctaac gcgacgctgt 7081 aacatttcaa attaaaccaa agggcgggcg cagtgcccgc cctttttaca taacggagta 7141 atccaaatga atgtagattc taatcttttt gaaaactggt ttgacacaac aagcgcgcaa 7201 ggtcagcaaa cctcgctttg ggataaactg gttaacgcgg gcggtcaagt aatcgacacc 7261 ctacctgata tcgtagacgg cgtggtcgat tcaaagttaa acgcggaaat caagaacaaa 7321 accgaaacga ctgaaaatat cacatcgact aacccgagtg tgtcaggttc agcaaatcag 7381 ctaacattct tgaaagaccc taaagtcatg tatggcggtc tagctatcgg cggtattatc 7441 gcggcggcta ttcttatcaa ggcgcttaaa taagggaggg cgcacaatgc cttttttatt 7501 acctattgca gttttagcgg gtacgggtgt ctttgcttgg aaagcgggcg acgccgtaaa 7561 caacgtttca gataagctag tcagtgccgc cattatcggt ggcgcggctt acctaattta 7621 caaagggagt aaataactat gcaagcagca ggcggcggcg gaatgtcagc gggttctagt 7681 gcgggcgcgg ttggtggcgc atctggctct agtatcggtg gcgtaaatat cgacctaagc 7741 ggtcgtggtg atatgaatgt tacatatcta gttggcggcg ctttagcggt tggtgcgctt 7801 ttcattttct taactaagcg aaagtagggt aaatcatgaa taagttcgag ccagtcccaa 7861 ttgatgaaat acttgaagcc atgccaaaga gtatccatag gcatacggat ttagtaaagc 7921 gtcaactcaa agcacagcac cagtacggtt ataaattccg tggtttgtac ataattttcg 7981 aaccgtggga cgatactcta atggttcaat gtgtcgaggg aacgggattg gatgaggact 8041 tcgtaaaagc cctttataag tgcgctataa ataacggctt cgaatacatc caatttgtaa 8101 cgccacataa agggctacat aaaatggtta aatccctcaa accggaacga atagaatttc 8161 gttatatttg taaactcgac gaggtggaat tatgagtttt tcacgaggcg gttcaagcga 8221 atcagccacc acaaacgaac aaaaccctat caccgcgact gataatgcta tggtcgccag 8281 tgagggtgga acaattacag tcactgacgg cggcgcgttc aacgtagtaa ctgacgcaat 8341 cgacgcaatc ggtgacgtag ttctaagcgc tgaatcactt gctaagaacg cgatgagttc 8401 aacggagtca atcgcgtctg actcaatcaa cgcgggactt gactatcatt cccaaacaac 8461 tggctttctt gagggaacgc ttgacaaggt tttcaacttt gtcggggata ccgtccagaa 8521 atcacaagat acaatttctg gaaatactca agcccttttt aattctgcaa aatcggatag 8581 cctaaaccgc tccgaagata tgcaacaaac gattcgaacg cttggtatgt acgcgactat 8641 tggtttagtc ggttatactg cttttcgatt cctaaagaaa taaggagtta atatgtctct 8701 tattcgtgac tatgatttga acatcgaatc aaacgctaca aaatcttttg ccaccactgg 8761 cactatgatt gcagttcgtc gcggtgacga ccctattcgc gttcgcgtaa aagcttacac 8821 caaagagggc ggaacgcttg taactgatag cgtcctatca gcgggtgaaa aagttcgaac 8881 gggtgaagaa tttaacttca tcgaaatcac taactatgag ctaaccaagc gacttgttcg 8941 agttacattg ggcgacggcg attttcaatc cgacgttgta gagggcgaag tcgtcgttcg 9001 aagtgatacc gataacccgc ttcaagttat ttcaaagcgc gcaccgcgtg atgtgttgtc 9061 agctttcatc acgcaccaaa agggaacgga tacccctgta accagtccag atggtacgat 9121 taaatttagt gcgcagaatc tgacaggcgg ggatatctat attttcattg gtaacgcgta 9181 tatttatcta gcgcctacgc aattgttttc aactgatatt gctcaagata ctcgcatcaa 9241 agtggcggga atttccgagg gcggtcagat ttctatgttc tgggttatcg aggggtaata 9301 ccttatgact ctaaaacctt tattaatagt gataccgcta ttaggagttt attacatgag 9361 taaacgctca cccgctttac acgcttcgcg aggttatcga aacaataacc cgcttaacat 9421 tcgtataggt tcttcaaagt ggaaagggga aattccaacc gataagaaca cagacggtaa 9481 atttgaacaa ttttataagc gctccgacgg ataccgcgcg gcggcgaaat tgattttgaa 9541 ttataaccgc ctatacaata ttttcactgt tcaacgtatt atcgagcgat gggctcccgc 9601 tatcgggaac gcccccgacg ggacgaaata caaaaaccat acgcatcaat acattgactt 9661 tgtttgtcgc aaaacaggat tcgacccgaa cacactaatt aacgggtcga acgtagggga 9721 gttggtttgg gctatggctc agttcgagaa catgccgcct cataccgatt cacttgactt 9781 tgttaaaaac tccgttagcg agttcaaaga tatcaactac taggaggctt tatgtttctt 9841 tcagcagttc cacaaaagaa agttaacccc gccagaattc agcaaacttt gtcggcctcc 9901 aacatggtaa gcgttacaat ccccgctaaa acttggcggg tgatgagcgc cgatgtgccg 9961 gataacgttg ctacgcttgt atttgtcatt acgcgtgatt actcttcagc acccgctgac 10021 actggtgtca ccattgaaag agttagcgac ggacacactg tttacgaggc taccgtatcc 10081 gactcgccga acacctccga atatgcaacg ctacaagttt cgcgtccgta tgagggttgg 10141 ggttctctca agatatataa taatactgct aacgctggac gtcttataat caacgcgtgg 10201 acgaataccg acctatacgc ataaggagtc attatgccta acgtacatcc taaaaagttc 10261 aatgtaatag cgggtattct cgcgggcgct ctagtcgggt tcggcggtgg ttcggttaat 10321 ctgaattaca acgcttcccc taatgaatta caactgctag actgtttaac aacaattgac 10381 aatatgatta agtcgcatga cgataatttg ggaatggtag tccagtctct tatggaatca 10441 aagattcaat cataacgttt cactgttttc gggtgtttgg cgctgctcct gcctttcacc 10501 cgccttttct cttggttcac acttcccctt tcttgtcaaa tttaaatctc ataaatgtga 10561 tgtatatccg caaatatgcg //