LOCUS MN180252 16547 bp DNA linear PHG 26-JAN-2020 DEFINITION UNVERIFIED: Wolbachia phage WO isolate Seq1WOMel_AMD1 genomic sequence. ACCESSION MN180252 VERSION MN180252.1 KEYWORDS UNVERIFIED. SOURCE Wolbachia phage WO ORGANISM Wolbachia phage WO Viruses; Caudovirales; Myoviridae. REFERENCE 1 (bases 1 to 16547) AUTHORS Mohanty,S. and Singhal,K. TITLE WO phage of wMel_AMD JOURNAL Unpublished REFERENCE 2 (bases 1 to 16547) AUTHORS Mohanty,S. and Singhal,K. TITLE Direct Submission JOURNAL Submitted (15-JUL-2019) Department of Biotechnology, Jaypee Institute of Information Technology, A-10 Sector 62, Noida, Uttar Pradesh 201309, India COMMENT GenBank staff is unable to verify sequence and/or annotation provided by the submitter. ##Assembly-Data-START## Assembly Method :: Velvet v. 1.2.10 Coverage :: 61x Sequencing Technology :: Illumina ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..16547 /organism="Wolbachia phage WO" /mol_type="genomic DNA" /isolate="Seq1WOMel_AMD1" /isolation_source="Drosophila melanogaster" /host="Wolbachia sp. wMel_AMD" /db_xref="taxon:112596" /country="India: Ahemdabad" /collected_by="Sujata Mohanty and Kopal Singhal" CDS 1..327 /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="QHJ75560.1" /translation="MRERFSDIEAVYFEEVRRHLGTDAAHCYGGFLAHLTAWCEENNI PYQGVPVKTIKRFITGKGNASKSEVIEAVKGKGFIPQDDNEGDALALMFYVNNFSKDF NMLDSA" CDS 454..1683 /codon_start=1 /transl_table=11 /product="DNA methylase" /protein_id="QHJ75561.1" /translation="MNLATHYYPIENLVEYDRNPRKNDDVVNRMCASIREFGFCIPIV AKSNGTVVDGYLRLKAARKLGMESIPVVLSDNLSEAQTKAFRLLANQSANWAKWDDDL LKLEIQELEDLQFDLKMTGFELEKVQRFLDDLDGENSEKEDFSDLAGDSKKVKITKPG DLWILGGHRIYCGDSCLVESFKAVLDDKMADITVCDPPYNVAYGDSQEREDKKILNDD QGEKYELFLYDICSHVLAYTKGAIYICASSSELATLQKVFEEAGGRWSTFIIWAKNHF TLGRSDYQRQYETILYGWKNGNKREWHGGRNQSDLWFYDKPTYNSLHPTMKPVELMER AIVNSSRPGDIVLDPFSGSGSTLIACERTGRICRTIELDPTFVDVTIKRWQVYTGREA ILSNTGKTFAQIQEEKR" CDS 1700..2191 /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="QHJ75562.1" /translation="MEGGKITQTEWARELGVSKQYVCYLVKKGIVELEDGLIDREQAN EAVAAIRDPSQPLRRKNPEGEEVGNNKLSMMLLKTRIKNEMERGRLLEAKAKAEIGEL ISVEEVKTEAFNVARVVRNNLLNIPDRVSALLASINDTEKIHETLTEEIRTALEELTQ SVF" CDS complement(2441..2560) /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="QHJ75563.1" /translation="MNTTFSAGEDEDSESIISLRYGVLSVLHFPGSDDAIVES" CDS 2559..4187 /codon_start=1 /transl_table=11 /product="terminase large subunit" /protein_id="QHJ75564.1" /translation="MKGAQIGGTEAGNNWIGYIIDQTPGPMLVVQPTVEMGKRWSKGR FAPLIEDMPCLRSKIKDPRSRDSGNTVQSKEFPGGTVVVTGANSPVGLRSMPVRYLFL DEVDAYPGDSGGEGDPVLLSIARTNTFTRRKIFLVSTPTVHGISRIEKEFESSDKRYF FVPCPHCDHYQVLKWSQIKWKDKDPSTAHYVCIEYSGKIENHQKTEMLARGEWRATRE EGKKEAKIGFHLSSLYSPVGWYSWKQAVEDYLHAKENEQLLKVWINTTLGETWVDKGE VPDWKQLFERRENFPIGMVPKGGKIVLTAGVDVQKDRLEAEVVAWGMGRESWSIDYQV LEGDTGSGEVWGKLSELLNHHFIGENGLEYMISMMAVDAGYATQEVYNWVRSHQGSGR VMAVKGVNKALVPLNSPSRVDVTVGGQKLKRGIKLWPVGVSILKSELFQLLNVLTEGA PGYCHFPEYPPEYFKQLTAEQLITKVVKGYTKQEWQKIRDRNEVLDCRVYARAASIAL GIDRWPESKWESLVGEKAKKSKKVRRSQWLSEKS" CDS 4187..4408 /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="QHJ75565.1" /translation="MYNQEYLTQVKEAIKKLQSGERVVSIAYGDHVVRYGEVQINDLL SLRQRIKAELKVAGVKRKIVISTNKGIDK" CDS 4454..4867 /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="QHJ75566.1" /translation="MIGNVERLQRELLEEMGSISLGSSNTRALEIVNQLGEDHINDTI MTLDGRQFTALDCAIMFDSQGNNPRINERPRINNNAFRELEGAIRSAGGRTSEELGRR QDIPYDRESFNEQVSTFLTDVTIEHVMAGRLHLMV" CDS 5068..5289 /note="RelB/StbD replicon stabilization protein(antitoxin to RelE/StbE)" /codon_start=1 /transl_table=11 /product="RelB/StbD replicon stabilization protein" /protein_id="QHJ75567.1" /translation="MVNSRVNVTFNPKTSQHLIELAKATKQSVQKLTEKLIKEAIELE AEDIAFSKIVRELDADDSEEVEDSEDIWK" CDS 5333..5578 /codon_start=1 /transl_table=11 /product="RelE/StbE replicon stabilization toxin" /protein_id="QHJ75568.1" /translation="MPSLPEAIKPKIKDAIREYLATDPIGNGVLLRNRLKGHRRIRVD DYRVVYRVNTAERKVTIVSIGHRDNIYKQAILDLLKH" CDS 5698..6072 /codon_start=1 /transl_table=11 /product="portal protein" /protein_id="QHJ75569.1" /translation="MLLKSFKQLFRKPKIKSSAWDASGSGRRLLHFQPEVGSINSLLS QSLEHLRSRSRDMVRKHSYAANIIDTIVSNCVGTGIKPQSKAKDGEFRKKVQELWLRW TDEADSSGVSDFMGYKLWFVGA" CDS 6069..7115 /codon_start=1 /transl_table=11 /product="portal protein" /protein_id="QHJ75570.1" /translation="MIEGGECFVRLRTRKREDGLCVPLQLQVLESEHLDNKSNQTLAN GNVIRNGIEFNRLGQREAYYLFREHPGEGSFGESVRVPANDVLHIYRPLRPGQIRGVP WLSSVLLKLYELDQYDDAELVRKKTAAMFAGFITRLDPEANIMGEGESNEHGVALSGL EPGTMQLLDPGEDIKFSEPSDVGGSYEAFMKQQLRAIAVGTGITYEQLTGDLSGVNYS SIRAGLIEFRRKCAMLQHNVVVFQLCRPVWNRWLELALLSGELDIGENWAKETAKEVK WIPQGCAYVDPLKDQQAQQMAVRNGFKSRSEVVSELGYDVEEIDQEIAEDQRRASELG LSFDSDVNSNAEMI" CDS 7217..7681 /note="head-tail preconnector protein GP5" /codon_start=1 /transl_table=11 /product="head-tail preconnector protein" /protein_id="QHJ75571.1" /translation="MIEQRSFELLSLHAGKHPTFKNIKHTAENNVEKIAIIPIHGILT KKPGAFDDMLGMTSYEKIREEIEKALIDEEVETIILDIDSPGGEVNGLFDLSDFIYEA RGLKKIVAIANDDAYSAAYAIASSAEKVLVTRTSGVGSIGVIASHIDQRALW" CDS complement(8410..9219) /note="putative prophage LambdaW1, baseplate assembly protein J" /codon_start=1 /transl_table=11 /product="baseplate assembly protein J" /protein_id="QHJ75572.1" /translation="MEQPNIIEPLNFEEIFSRMKEELVSRDESFTALVESDPAIKILE VAAWRELLLRERINEAARSNLLKFARGEELDNLAEFYGAERQDGEEDERFRKRIKAKI KGWSTGGSKEHYRYHALSADRRVKDALVESKVPGSVEISILSTELSTNGIASEELLDI VRKRVTRDDIRVLTDTITVVGCNIIEIDIHSKISIKRPDIIETVKKKFIEKFEATKRL GWKVTKSWIIANLFVEGVENVELIEPRKDVVVLGNECVCLGKLEIELMGKS" CDS complement(9222..9548) /codon_start=1 /transl_table=11 /product="baseplate assembly protein" /protein_id="QHJ75573.1" /translation="MNASTGKELEGLNHLKQSIVDILTTPIGSRVMRRDYGSRLLELV DKPINRDLTLEIYAAVAEALQKLEQRFKLEKVKITEVKEGKVNISLDGIYLPNGKNIH FDGIMV" CDS complement(9554..9796) /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="QHJ75574.1" /translation="MVCIGDYCSGIPAHVCMSGSSDVFVNGRSVCRKGDILTLGEKLT QGSNSVFVNDIGITRTGDLVSCGFHVMGDSKNVFAE" CDS complement(9811..10101) /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="QHJ75575.1" /translation="MVCANIDEQVIVLSPYGELSLGVVLPAIYQEKYPPPENKKEINS VKFQDGTKLSYEQEKHHLKIDVVDKITLKVGESSMKITKRGIKLKAKRIDLN" CDS complement(10071..10274) /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="QHJ75576.1" /translation="MLEHNFAISELNRKLANIIRIGLVKEVDYEKARVRVKIGEFLTD WLPWVTARAGEDRSWFARILMSK" CDS complement(10261..10734) /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="QHJ75577.1" /translation="MFWRDLHQKICKTLKEEIPAIQTCEVYPAVRREIIAPAAFVELA SLEPGKDPGTEELALRARFEVRVVVDGTVEDSQVVVRSLAAEIARVVNKNTWDVENVS AGEFISAGGDDFKPELDAYLVWLVEWVHELHTGQSMWLETGIIPHTISVGENVRA" CDS complement(10741..11238) /note="putative prophage LambdaW5, minor tail protein Z,putative" /codon_start=1 /transl_table=11 /product="putative minor tail protein Z" /protein_id="QHJ75578.1" /translation="MRINIEVTGSIIQSIDAERKKVEKATVRALNKTALWVRSQTVKQ VSEEKQIPKKAMRKKLSVDKANRKRLWSVVKLSSQWIGVAKLGSIKQTKIGAKVGSRT YEGAFIATMKNGHIGIFRRRYTTSLPIDEIKINTHARETMKELVSNEVERVFEKYFDH ELNFI" CDS complement(11239..11382) /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="QHJ75579.1" /translation="MLKLKTSDAFDKIQGDIFIIDKRKYKVYSPPLRDNSGMLWKIQA SGV" CDS 11551..12585 /note="Mobile element protein" /codon_start=1 /transl_table=11 /product="mobile element protein" /protein_id="QHJ75580.1" /translation="MELYMINNFLGIDISKDRFDVSLSFISNKGKRETRKRSFKNNDH GFQGLLSFLQKHNVEQVKACMEATGCYSEALAEFLHNTGHFVSVVNPACIRFYAKSKL IRQKNDQTDAEIIADYCQRQEPIRWAPPSPELKKLKHLYRCSAALKDELTLVNNHLEK KERLPKEVANAWEDLAMNIKQKIERIKNSLRELLNKHKELLENFQLLLTIPGIGEESA VAILAEVPNIKAFKDARQLAAYAGAIPRNITSGTSVHAKPRLSKSGSRTLCKAMYFLA IVAKNHNPTIMTFCKRLKEKGKHNMAIVGAAMRKLLHIVFGVLRSKKAFDPDHIKNYR ARSLAESLVL" CDS complement(12771..13031) /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="QHJ75581.1" /translation="MFRDIKRLFADCFAHLGQQALYESKDKSYMVQILKQQPDKLYEI GEGQFVGEILILEVSIFDVLQPVVDTVLQVNKKITKIVQAVS" CDS complement(13122..14123) /note="elements of external origin; phage-related functions and prophages" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="QHJ75582.1" /translation="MQNPFTNSAFSMTELTKAINILPINYGRTESLNLFPSRSVRFRH ITIEEQNGVLSLLPTQVPGAPATVGKRGKRKIRTFTIPHIPHDDVVLPEEVQGIRAFG SENELKALANVVTDHLELMRNKHAITLEHLRMGALKGIILDADGSELLNLYNEFEITP KVVNFALGIATTDVKRKCMEVLRHIEDNLSGEYMTGVHALVSPEFFDALTSHSKVKEA YERWQEGAALRNDMRSGFTFCGITFEEYRGQATDPEGTVRRFIEKDTGHCFPLGTAST FTTYFAPADFNETVNTLGQPLYAKQEPRRFDRGTDLHTQSNPLPMCHRPGILVKLAA" CDS complement(14201..14572) /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="QHJ75583.1" /translation="MSCITEQNNLGDLLKYEASNLYSRDQITVTKGQNLSLGTVVGCN IEDNVIKIINPTATDGTQTAIGVIASDVNVKENTKAVIITRIAMLADHAVVWPANITE EQKAAAIKQLEARGIIIRKGV" CDS complement(14547..15608) /note="Mobile element protein" /codon_start=1 /transl_table=11 /product="mobile element protein" /protein_id="QHJ75584.1" /translation="MRYIGVDLHTNSFTACYLEQEKPEYIKTFRLQDLGNFIKNLQET DEVALEATGNSCFFYDEVSPYVKRVVVVAPLQFEVIRRSVNKTDKHDARAIAFFLSKD MLPEAKCKSKQCQQLASLLKTRDQLVKSRVSLINKIHGLFNYHGIKIKKEVLTIKAGF ECSLGKHDWSHLEKVEIEVISSNLEVIRESLKKLEKEIIAFAQQLPGFNNLISIKGIG AISAAVFVATIGDINDFSNPEKLTAYFGVVPRVSQSNQQCTIGRITKRGSKIGRTSLV QCTWIAIRYSPYLKSFYERVKKSVVLLRLLLLLLGSFSQLFSILLKTTGFSKILQNMK FLIDNNHRRNIYELYNRTK" CDS complement(16038..16229) /note="head-tail preconnector protein GP5" /codon_start=1 /transl_table=11 /product="head-tail preconnector protein" /protein_id="QHJ75585.1" /translation="MSKMPEKIGEFIEQDVNAKQAQEILMSILAERTKKTEILSTIPQ SSSEDLMMQVARTRAQSGI" CDS complement(16251..16547) /note="head-tail preconnector protein GP5" /codon_start=1 /transl_table=11 /product="head-tail preconnector protein" /protein_id="QHJ75586.1" /translation="MESLQDEVDRLYEMFVELVARNRNLSTEKIKSTEAELYFGEKAI EIGLADEVITYSEFIDRRNNMSKAEFNYKGHTIDADELMEESKRIGYERCRKEV" ORIGIN 1 atgagggaaa gattttcaga tattgaagca gtgtattttg aagaagtaag aagacacctt 61 ggaactgatg ctgctcattg ctacggaggc tttctagctc atttaacagc ttggtgcgag 121 gaaaacaata taccttatca aggtgtacct gtcaagacga taaaacgctt tatcaccggt 181 aagggtaatg caagtaaaag tgaagttatt gaagctgtga agggaaaagg gtttattcct 241 caagatgaca atgaggggga tgctttggcg ttgatgttct atgttaataa ttttagtaaa 301 gattttaaca tgctagatag cgcataggaa gtgggtcctt cccgccagca aggctgattt 361 gggagcgaaa ccccggacct tctccagcgt cagacgtttt ttaattatta actttttgtt 421 aagaatttaa acatagcaat ttaatacact aatatgaact tagcaacaca ctactatcct 481 attgaaaatc ttgtcgaata tgatcgcaac cctcgcaaga atgacgatgt agtaaacaga 541 atgtgcgcct ctattcggga atttgggttt tgtattccaa tagttgcaaa aagcaatggc 601 actgtggttg atggttattt acgcctcaaa gcagcaagaa aactgggtat ggaaagtatt 661 ccagtagtcc tcagtgataa cttaagtgaa gcccaaacca aagcttttcg gttactagca 721 aatcaatcag ctaactgggc aaagtgggac gatgatcttt tgaagttgga aattcaagag 781 ttagaagatt tgcagtttga cctaaaaatg actggatttg agctggaaaa ggttcaacgt 841 tttcttgatg atttagatgg agaaaacagt gaaaaagaag atttttccga cttagccggt 901 gatagcaaaa aggtaaaaat aacaaaacca ggggatctat ggattttagg tggtcatcga 961 atctactgtg gtgatagttg tctggttgaa tcatttaaag cggtattaga cgataaaatg 1021 gcagatatta ccgtgtgtga ccctccgtat aacgtagcat atggtgacag tcaggaaaga 1081 gaagacaaaa aaatactaaa cgatgatcaa ggtgaaaaat acgaactttt tctgtatgac 1141 atttgttctc acgttttagc gtacaccaaa ggtgcaattt acatctgtgc atcatcatcc 1201 gagctggcaa cgctgcaaaa ggtatttgaa gaagcaggtg gtcgttggtc gacatttata 1261 atttgggcaa agaatcattt tacgctagga agatctgatt atcaaagaca atacgaaaca 1321 atactctacg gttggaaaaa tggcaataag cgtgagtggc atggaggacg taatcaaagt 1381 gatctctggt tttacgataa gccaacgtac aactcactgc acccaacgat gaaaccagtg 1441 gagttaatgg agagagcgat agtaaacagc agtagaccag gggacatagt tcttgatcca 1501 tttagcgggt ctgggagtac gttgattgca tgtgagagga caggaagaat ctgtagaacg 1561 atagagctag atcctacgtt tgtagatgtg accataaaac gttggcaagt atacacggga 1621 agagaagcga tactttccaa tactggcaaa acctttgccc aaattcaaga agaaaaacgg 1681 taaaagaagg gagaaaaaag tggaaggagg aaaaatcacg cagacggaat gggcaagaga 1741 actaggagtt tcaaagcaat acgtctgtta cttagtaaag aaaggaatag ttgagctgga 1801 ggatggtttg attgaccgag aacaagcaaa tgaagcggtg gcagcaatac gagatccaag 1861 tcaaccactg aggagaaaaa atccagaagg tgaagaagtg ggaaataata aactttccat 1921 gatgttgcta aaaacgcgga taaaaaatga gatggagcgg ggaagactgc tcgaggcaaa 1981 agcaaaagct gagattggtg aacttatctc agtagaagaa gtaaagactg aagcatttaa 2041 tgttgcaaga gttgtccgta ataatttgct taacattcca gatagggttt cagcgttact 2101 agcatcgata aatgacacag agaagatcca tgagacactg actgaggaaa tcagaacagc 2161 tttagaagag ctgacacaaa gtgtatttta atgactacat ataaattact gaaaaagcgt 2221 cttatcgcct tggtgagcct aattcacata ttagttgcaa taatgatttt aagtaaagcg 2281 tcagcagata cttattgtag agggaaaata cctttttctc ttagctgtgt aaaagtataa 2341 aaattgcagc agatggcatt atgatatata gcagtagctt ttatacaggg ttaaagccag 2401 atccactgct caaagtatca gagtgggcag atagaaatcg tcaactttca acgatagcat 2461 catcagagcc ggggaaatgg agaacagaga gaacgccgta ccttaaggaa ataatggatt 2521 cactatcttc gtcttcaccg gccgaaaaag tggtattcat gaaaggagcg cagataggcg 2581 gaacagaagc tggaaataac tggataggat atataatcga ccaaacacca ggccctatgc 2641 tggtagtaca gccaacagta gaaatgggca agcgttggtc aaagggaaga tttgcgccgc 2701 tcattgaaga tatgccatgt ttaaggagta aaataaaaga cccaaggtcg agagattcag 2761 gcaacactgt acaaagtaag gaatttcccg gaggaacggt agtagtaaca ggtgccaaca 2821 gtccagtggg tttacgttca atgccggtaa gatatctttt tttagatgaa gtagatgcat 2881 atccaggaga ttcaggagga gaaggagatc cagttttact cagcatagcc cgaactaata 2941 catttacgcg gagaaaaata ttcttagttt caacaccaac ggttcacgga ataagcagaa 3001 tagagaagga atttgagagt tcagacaaac gatacttttt tgtaccttgt ccacattgtg 3061 atcactatca ggtgctgaaa tggtcacaga taaagtggaa agataaagat ccctcaacag 3121 cccattatgt gtgtatagaa tacagcggca aaatagaaaa ccatcaaaaa acagagatgc 3181 tagctagagg tgaatggaga gcaactaggg aggagggaaa aaaagaggca aaaatagggt 3241 ttcatttatc gagcttgtat agcccagttg gttggtatag ttggaaacaa gcagtggaag 3301 attatctgca tgcaaaggaa aatgaacaat tactaaaagt ttggataaat acaacactcg 3361 gagaaacctg ggtagataag ggagaagtac cagactggaa gcaactattt gagcgtcgag 3421 aaaattttcc gataggcatg gtgccaaaag gtggaaaaat agtgttaacg gcaggggttg 3481 atgtacaaaa agaccgctta gaagcagaag tagtagcatg gggtatgggt cgtgagagtt 3541 ggtcaataga ctatcaggta ttagaaggtg atactggaag tggagaagta tgggggaaac 3601 tgtcagagct cttgaatcat cattttatcg gtgaaaatgg gcttgaatac atgataagta 3661 tgatggcggt agatgctggg tatgcaacac aggaggtata caattgggta agaagtcatc 3721 aaggatcggg aagagtaatg gcagtgaaag gggtaaacaa agccctagtg ccgcttaaca 3781 gcccaagtag agtagatgta acagttggcg gacaaaagct aaaaagagga ataaagctct 3841 ggccagttgg agtatcgata ttaaagtcag aattgtttca gttgctgaat gttttaacag 3901 aaggggcgcc gggatattgt cattttcccg aatatccacc tgaatatttt aagcagctaa 3961 ccgcagagca actaattacc aaagtagtaa aaggatatac caaacaagag tggcaaaaga 4021 taagagaccg aaatgaagta ttagattgca gagtttacgc aagagcagcg tctattgccc 4081 tggggattga tcgttggcca gagagtaagt gggagagttt agtaggtgaa aaagctaaaa 4141 agtcaaaaaa agtgagaaga agtcaatggt tgagtgagaa aagttgatgt acaaccaaga 4201 atatttaact caagtaaaag aagcgataaa gaagctacag agcggggaaa gagtagtatc 4261 gattgcatat ggcgatcacg tggttaggta cggagaagtg cagataaatg acctcttgag 4321 cttaagacaa cgcattaaag ctgagctgaa agttgcaggt gtgaagagaa agattgttat 4381 ttcaacgaat aaaggaattg acaagtgaaa atgtaatact gtacgttaga taatttataa 4441 atataggggt aacatgatag gaaatgtaga aagattgcaa agagaactac ttgaagaaat 4501 ggggtctatt tctttgggga gtagtaatac aagggcatta gagattgtca atcaattagg 4561 agaagatcac attaatgata ctattatgac tcttgatggc aggcagttta cagctttaga 4621 ttgtgcaatt atgtttgaca gtcaaggtaa taatccaaga ataaatgaaa ggccgagaat 4681 aaataacaat gcatttagag aattagaagg tgctattagg agtgctggag gaagaacatc 4741 agaagaattg ggtagaagac aagatattcc ttatgatcga gaaagcttta atgagcaagt 4801 gagtacgttt ttaacagacg ttactataga gcatgttatg gcagggagac tacatctaat 4861 ggtttaaatt gaatgcggaa aaaattatcc taacggaaaa aagctgagga aattggttaa 4921 ccaattgtta agtatttaaa ggagatccgg gacgtaaaga ggagagttgt gatttcgacg 4981 aacaaaggga ttgtataaag aaatacttat tgaaaaatta aaagagatac tgtataatta 5041 gtgtaacatc taggtttggt taaacatatg gtaaactcaa gagttaatgt aaccttcaat 5101 ccaaaaactt cacagcatct tattgaacta gctaaagcaa ctaagcagtc tgttcaaaaa 5161 ttaacagaaa agctaatcaa agaagcaatt gagcttgaag cagaagacat tgcgttttcc 5221 aaaattgtta gggagcttga tgctgacgat tcagaagaag tagaggatag tgaagacatc 5281 tggaaataag cggtacacta taaaatacct taagcatgtt cttaaaagga atttgccctc 5341 cttaccagaa gctataaagc caaaaattaa agatgcaata agagagtatc ttgcaactga 5401 tcccattggt aatggtgtat tgctacgtaa tagattaaaa ggacacagaa gaataagagt 5461 tgatgattat cgtgtcgtct atcgtgtaaa tactgcagaa cgtaaagtaa ctattgtttc 5521 aataggacac agggacaaca tttacaagca ggcaatatta gatctactaa aacattaaga 5581 aaagacattt gtgcaggaat ctcttgcata aatagaaaat gtcaacagct tgttttctcc 5641 agcagcttga cgtaatttta aatagcgcaa caatttattg cccaattaat agtctaaatg 5701 ttactcaaat cattcaaaca attattccgc aaaccaaaaa tcaagagctc agcctgggat 5761 gcgtcaggct caggaagaag attattgcac tttcaaccag aagtaggaag tataaatagc 5821 ttactttccc agagccttga acacctgcgt agtcggtcac gtgacatggt aagaaagcac 5881 tcctatgcgg caaacataat cgatacgata gtgagtaatt gcgttggaac gggaataaaa 5941 ccgcaatcaa aagccaagga tggagaattt cggaaaaaag tgcaagaatt atggctgaga 6001 tggacagatg aagcagacag tagtggagta agtgatttta tgggttacaa gctttggttt 6061 gtaggagcat gatagaagga ggagaatgtt ttgtaagact tcgaacaaga aaacgagaag 6121 atggtctttg tgtgccgttg caattacaag tacttgagtc agagcattta gacaataaga 6181 gcaatcaaac ccttgccaat ggtaatgtaa ttagaaacgg gattgaattt aaccgacttg 6241 ggcaaagaga agcgtattac ctttttcgag aacatccggg tgaaggctcg tttggagaat 6301 cagttagagt gccagcaaac gatgttttac atatctatag accactaaga cctgggcaga 6361 ttcgaggagt accatggttg tccagtgtac tgttaaagct ctatgaactt gatcaatacg 6421 atgatgcgga attagtcaga aaaaagactg cagcaatgtt tgcgggattt attaccagac 6481 ttgatcctga agcaaacatc atgggagaag gagagagtaa tgaacatgga gtagcgctgt 6541 ctggcttaga acctgggact atgcaattac ttgatcccgg agaagatatt aagttctcgg 6601 aaccatcaga cgttggggga agttatgaag cattcatgaa acagcagctt agggcaatag 6661 cagtaggcac agggataact tacgagcagc taacaggaga tctaagtggt gtcaattatt 6721 catcaataag agctgggtta atagagtttc gtaggaagtg cgcgatgtta cagcataacg 6781 tggtagtatt tcagctttgc cggccagtat ggaataggtg gctagaatta gcactactct 6841 ctggagaact ggacataggt gaaaattggg caaaagaaac agcaaaagag gtaaaatgga 6901 taccgcaagg gtgtgcttac gtagatccac taaaagacca gcaagcacag caaatggcag 6961 taagaaatgg atttaagagt cgatcggaag tggtttcaga actaggttat gatgtagaag 7021 aaatagacca agaaattgct gaggatcaaa gacgtgcgag tgaactgggc ttaagttttg 7081 attctgatgt gaattctaat gctgaaatga tataaaattc tatttctatg acaaatgggt 7141 ttttattgcc ccagacacaa ataaaaaatt ttaaaaacat atgacgacac aaataaattg 7201 gatgaataag ccgatgatga tagagcaaag gagttttgaa ttattgtcat tacatgctgg 7261 aaaacaccct acgtttaaaa acataaaaca cactgcagaa aacaacgtag aaaaaatagc 7321 aataatacca atacatggaa ttttgaccaa gaaaccagga gcttttgatg acatgttggg 7381 aatgacatca tatgagaaga tacgtgaaga gatagaaaaa gctttaatag atgaagaagt 7441 agaaacaata attttggaca tagacagccc tggaggggaa gtaaacggtt tattcgacct 7501 ttctgatttt atttacgaag caaggggatt aaaaaagatc gtagcaatag caaatgatga 7561 tgcgtattct gcggcgtatg caatagcgtc aagtgctgaa aaagtattgg taacgaggac 7621 ttcaggagtt ggaagcatag gagtaatagc aagtcatata gatcaaagag ctctgtggta 7681 gcggatatag cgctttgaag ttataccaca catgattcct ttcataaata ccgttatagt 7741 tcttagtatg aggctcggtt tctccaagta ttgctacatc taggcgatat atatcattgc 7801 tcaaagcttg ttcataatga ttgcgtagat agttctctat aatcctaaca actggaccac 7861 tagacctgaa aaaatttact ctcccaaatg ataacactgg tccatctttt tcgatctttg 7921 taccagagta gtctgataat aagctgccaa aaaagctttc atctaaaata aatcttccaa 7981 tattatagtg gtcattgaaa agcctcatta gcctcgatct tgctggcaat gatagttttg 8041 ctagttctac tactgcatcc acgaagaagt cgttcggaac atctcttatc cctacctgca 8101 actcaaaaaa gtgtttaccg ggtggctctt caatgatcgt aatatcttct atatttgccc 8161 actttaatgc tattttcagt gaggcaggtg taccacgcag tctttgaaat tttattcctt 8221 ctcttatcgc ctttcctttg tcttttaccc aacgcagaat ttcccccaat ccatattctt 8281 ctattatcca cggtagtatt tcatctttag aattaaactt aaatcctttt atcctgcttg 8341 gatcaacttt gtaatcgata gcctctacta tggctctttc ttcttttgtg ctattaggtg 8401 gaagtaacat tagcttttgc ccattaattc gatttctagc ttgcctaaac aaacgcactc 8461 attccccagt accacaacat cttttcttgg ctctattaat tccacgtttt ctaccccttc 8521 tacaaataga tttgctatga tccatgattt tgtcaccttc catcccaatc ttttcgttgc 8581 ttcaaatttt tcgataaatt tcttcttcac cgtttcaata atatccggtc ttttaatgct 8641 aattttgctg tggatatcta tttcaataat attgcaacca actactgtta tcgtatctgt 8701 caataccctt atatcatccc tggtgactcg ctttcttaca atatcaagta gttcttcaga 8761 tgctatgcca tttgtggata actccgtgga taaaatcgag atctctacac tccctggcac 8821 ttttgattca accagcgcat cctttactct tctatctgct gagagtgcat gatatcgata 8881 atgttcttta cttcctccag ttgaccagcc ttttattttt gccttgattc tttttctaaa 8941 tcgttcatct tcttctccat cttgcctctc tgctccataa aactcagcta aattatcaag 9001 ttcttccccc cttgcaaact tcagtaagtt gctccttgcc gcttcgttta ttctttctct 9061 cagcaaaagt tctcgccatg ctgctacctc taaaatcttt attgctgggt cactttctac 9121 taatgccgta aaactttcat cacgacttac taattcttct ttcatccggg aaaaaatctc 9181 ttcaaagttc agtggttcga taatatttgg ctgctccatc tctataccat aatcccatca 9241 aaatgaatat ttttcccatt tggtagataa ataccatcta atgaaatatt gacctttcct 9301 tctttcactt ctgtaatttt tactttttct aacttaaatc tttgctccaa cttttgtaga 9361 gcttctgcta ctgctgcata gatttccaaa gttaaatccc tattaattgg cttatctact 9421 aattcaagta gtcttgaccc ataatccctt cgcataacac gtgatcctat cggcgtggtc 9481 agtatgtcaa ctatcgattg ctttagatgg tttaatcctt ccaattcctt tcccgtgcta 9541 gcattcatgc ctctcattct gcaaatacat ttttgctatc acccatcaca tgaaaaccac 9601 acgataccaa gtcacctgtt cttgttatgc caatatcatt aacaaatacg ctatttgatc 9661 cctgagttag tttctcccct agagttaaga tatctccttt tcgacacaca gatctaccgt 9721 ttacaaaaac atcgctgctt ccactcatac aaacatgtgc tggtattcca ctgcaataat 9781 cccctataca aaccacagct ttagccatac ttaattaagg tcgattctct ttgctttgag 9841 ttttatgcct ctcttggtaa tcttcatact tgattctcct actttcaacg ttattttatc 9901 cactacatct atcttaagat ggtgcttttc ttgctcatat gataactttg tcccatcttg 9961 aaattttaca ctatttattt cctttttatt ctccggaggg ggatatttct cctgatatat 10021 tgctggtaat actaccccta atgacaactc tccatatggc gataatacta ttacttgctc 10081 atcaatattc gcgcaaacca acttctatct tctcctgctc ttgccgttac ccacggaagc 10141 caatccgtta aaaattcccc tatttttacc cgcactcttg ctttttcata atctacttct 10201 ttaactagac ctatacgaat aatgttcgct aacttcctat tcagctctga aattgcaaaa 10261 ttatgctcta acattttcgc caacacttat tgtatgtggt atgataccag tttctaacca 10321 cattgattga cctgtatgta gctcgtgaac ccattcgacc aaccacacca aatatgcatc 10381 caactctggt ttaaaatcat cccctcctgc tgatataaat tcacctgctg aaacattttc 10441 cacgtcccaa gtattcttat tcactaccct tgcaatttcc gctgccagtg accttaccac 10501 tacctgagaa tcctcaactg ttccatcaac taccactctt acctcaaatc ttgccctcaa 10561 cgctaattcc tcagtccccg gatctttccc tggttctaaa cttgcaagct ctacaaatgc 10621 tgctggcgct attatttccc tacgtactgc tggatacact tcgcacgttt gtattgctgg 10681 gatttcttct tttagcgttt tacaaatctt ctgatgtaga tctctccaaa acatacgctc 10741 ttaaataaaa ttaagttcat ggtcaaaata cttctcaaat actctctcta cttcattaga 10801 gactaactct ttcattgttt cccgagcatg ggtatttatt ttaatttcat ctattggcaa 10861 agacgtggta taccttcgtc tgaagattcc tatatgacca tttttcattg ttgctataaa 10921 tgctccttca tacgtacggc ttcctacttt tgctcctatc tttgtctgtt ttatactccc 10981 aagttttgct actcctatcc actgcgaact gagctttact actgaccata agcgctttct 11041 atttgcttta tccacactta acttttttct cattgctttt tttggtattt gtttttcttc 11101 gctaacttgt ttgacagttt gtgaccttac ccatagtgct gttttgttta gtgccctcac 11161 cgttgctttt tccacttttt tcctttcagc atctatactt tgtataatac taccggtgac 11221 ttcgatgtta atacgcatct acacccctga cgcttgaatt ttccatagca ttcccgaatt 11281 gtccctaagt ggtggtgaat atactttgta cttacgctta tcaataataa agatatctcc 11341 ttgtatcttg tcaaaagcat ctgatgtttt aagcttaagc attaggtgga gaagggaaga 11401 actttgaata tctccttaga tataaaatct gtgtggtaga caagagttcc cttctcaaaa 11461 gtataggctg aacggtatcg tagaaagacc taaatggtta taattctgta tccacaaggt 11521 tagccttact tttcatttta atagtaacgt atggagttat atatgatcaa caactttttg 11581 ggtatagaca tatcaaaaga tcgctttgat gtttctcttt cattcataag caacaaagga 11641 aaacgtgaaa ccaggaaaag gagtttcaag aataatgatc atgggtttca aggtttactg 11701 agctttctac aaaagcataa tgtagaacaa gtaaaagcct gcatggaagc tactggttgt 11761 tatagtgaag ccttagctga atttctacac aatactggac attttgtgag tgttgttaat 11821 ccagcttgta taagatttta tgcaaaaagt aagcttatac gacaaaagaa tgatcagact 11881 gatgcagaga taattgccga ttattgccaa agacaggaac ctattcgttg ggcaccacct 11941 tctcctgagt taaagaaatt aaagcatctt tatcgttgct cggctgcgtt aaaagatgaa 12001 ttgacattag taaataacca cttagaaaaa aaagagagac tgcctaaaga agttgcaaat 12061 gcttgggaag accttgcaat gaacataaaa caaaaaatag aaagaataaa aaactcctta 12121 cgtgaactat taaataaaca caaagaattg ttggaaaatt ttcaacttct attgaccatt 12181 ccaggaatag gagaagaatc agcagtagct attttggctg aagttcctaa cataaaagct 12241 ttcaaggatg ccaggcaatt ggcagcatat gctggagcta taccacgaaa tataacgtca 12301 ggtacgtctg tacatgccaa gcccagatta agtaaatccg gttcacgaac attatgtaag 12361 gcaatgtact ttcttgccat agtagctaag aatcataatc ctactattat gactttttgc 12421 aaaaggttaa aagagaaagg caagcataac atggccattg tgggagctgc aatgcgtaag 12481 ttactccata ttgtttttgg tgttttaagg tcaaagaagg cttttgatcc agatcatatc 12541 aaaaactaca gagctaggag tttggctgaa agtttagtgc tttaaaatca aaaaacgctc 12601 caattatgta aaatagcatc ttacacaaat tttgtaactg gttagtgaat atgttgataa 12661 gtaaattaat tttatacgta caaaaaagcg gaaatttact tatcaacata ttcactaact 12721 ataatctgtg taccgttaca caaaatgtga attggaagta ttttttaatt ttatgataca 12781 gcttgaacaa tttttgtaat ttttttgttg acttgcaaga cggtatctac tactggctgc 12841 aatacatcga atatactcac ctccaaaatc aatatctccc ctacaaattg tccttcacca 12901 atctcgtata atttatctgg ctgttgtttt aatatttgca ccatatatga cttatccttt 12961 gattcataca acgcttgctg ccctagatgt gcaaaacaat ctgcaaataa tcttttaata 13021 tctctaaaca taatttttta gttgcttttt attctttagt tgatataatg cctcctgttc 13081 ggggtattaa aaacaatctg cacttagctt tacctcacct gttatgcagc aagttttaca 13141 agtatacctg ggcgatggca cattggtaga gggtttgatt gggtatgaag gtcagtccct 13201 ctatcaaatc ttcttggctc ttgttttgca tagagcggtt gtcctagtgt atttaccgtt 13261 tcattaaagt ccgctggcgc aaagtaagta gtaaatgtgc ttgctgtacc aagtggaaaa 13321 cagtgacctg tatctttctc aataaatctt ctcacggttc cttcaggatc agttgcttgt 13381 cctctgtatt cctcaaatgt tattccacaa aacgtaaatc ctgatctcat atcattccga 13441 agcgctgctc cttcttgcca tctttcatat gcttctttta ctttactatg cgaagttaat 13501 gcatcaaaaa attcagggct taccaaagca tgtactcctg tcatatattc accacttaga 13561 ttatcttcta tatgccggag tacttccata cacttacgct ttacatctgt tgttgctatt 13621 cccagtgcaa aatttactac ttttggcgta atttcaaatt cgttgtaaag atttaacaat 13681 tcactgccat cagcgtccag aataatccct ttcaacgccc ccattcgcaa atgttctaac 13741 gttatcgcgt gcttgttcct catcaattcc agatgatctg ttaccacgtt agccaacgct 13801 ttcagttcat tctctgaccc aaatgccctt atcccttgca cttcctcagg taacactacg 13861 tcatcatggg gaatatgtgg tatcgtaaac gttcttattt ttcgttttcc acgttttccc 13921 actgttgctg gtgctccggg aacttgcgtt ggtaataggc tcaaaacacc gttttgctct 13981 tctatcgtaa tatgtcggaa tcttaccgat ctacttggaa ataaatttaa gctttctgtt 14041 cgaccataat ttatcggcaa tatgtttatt gctttagtta attccgtcat actaaatgct 14101 gaatttgtaa atggattttg catgtttttt cccctctaat gctgtagatg tttgttggct 14161 tttaagccct ttgcgaatga ttaagatgtt tgctgggctt ctaaacccct ttgcggataa 14221 tgatgcctcg tgcttcaagt tgctttattg ctgcagcttt ctgctcttct gttatatttg 14281 ctggccacac aactgcatga tctgctagca ttgctatacg tgtaatgatt actgctttgg 14341 tattttcttt cacgtttaca tcacttgcta tcactcctat agctgtttgc gtaccatctg 14401 ttgcagttgg attgataatc ttaattacat tatcttcaat attacagcca actacagtac 14461 ccaagcttag gttttgcccc tttgttactg ttatttggtc tcttgagtat agatttgatg 14521 cctcatactt taataggtca ccaaggttat tttgttcggt tatacaactc ataaatgttt 14581 ctcctatgat tgttgtcaat aagaaatttc atattttgta aaatctttga aaatccagtt 14641 gtttttaaga gtatagaaaa tagttgtgag aaacttccta gcagtagcaa taatagcctt 14701 agcagaacca cgcttttttt cacacgctcg taaaaacttt tcagataagg actataacgt 14761 atagcaatcc aagtacactg tactaaagaa gtacgcccaa tttttgatcc acgtttagta 14821 attcttccaa ttgtgcattg ttgattggat tgagaaactc gcggtacaac tccaaaataa 14881 gctgtaagtt tttcaggatt actaaaatca ttaatatcac caattgttgc aacaaaaact 14941 gctgcagaaa tcgcaccaat acctttaatg ctgatgagat tattaaatcc aggaagctgc 15001 tgagcaaaag ctataatttc cttttcaagt tttttgagac tttctcgaat gacttccaga 15061 ttactgctaa tcacttcaat ctcaaccttt tccaagtgac tccaatcatg tttaccaaga 15121 gagcattcaa atcccgcttt gattgtcagt acttcctttt taatctttat tccgtggtaa 15181 ttgaaaagtc catgtatttt attgattaaa gatacccgtg attttactaa ctgatctctt 15241 gttttaagaa gagaagctaa ctgttggcat tgtttgctct tgcattttgc ttcaggcaac 15301 atatctttgc tgagaaaaaa agcaatagct cttgcatcat gtttatctgt tttgttcaca 15361 gaacgacgaa taacttcaaa ttgcaaggga gcaaccacaa caacgcgctt aacgtaaggt 15421 gacacttcat cataaaagaa acagctattt cccgttgcct ctaaggctac ttcatctgtt 15481 tcttgaagat ttttgataaa attacctaaa tcctgtaagc gaaacgtttt aatatactct 15541 ggtttttcct gttctaagta gcaagcagtg aaactattag tatgtaaatc aacacctatg 15601 taacgcataa atcttctccc ttattgaata ctccaacata tcagagtaga atattaccgg 15661 ttggtccatt ctcctatacg cggtcactaa attttatagg ccgcacgatg gttttcggtg 15721 gcaggggcga ttaatctcct tcacgaggtc tttttcctat actgcaaaaa gcagaaaggg 15781 cctcacaaac attttcaatc tgccacctat ccactctgag aaggtattat cttctcatgc 15841 ttccaaccaa gcaataacta ttcataccat ctccttaatt gttttaccgc cgcttttgtt 15901 agtgttatat acggcggtta caagatttgt ttaccgcggt gtttaaagtt gctatacgtc 15961 gcggtgaaaa ctttataatt gtctattctg gcggacttgt ttaccgcggc atttgtaatt 16021 gctggtacct aaatttctca aatgccagat tgcgctcgag ttcttgctac ctgcatcatt 16081 aaatcttctg atgaactttg tggtattgtg ctcagtatct ctgtcttctt cgttctctcc 16141 gccagtatcg acattaatat ctcttgggct tgttttgcgt ttacatcctg ctcaataaat 16201 tctcctatct tctctggcat ctttgacaaa ttacacaatc ttattacctc tcatacttcc 16261 ttgcggcacc tctcataacc tattcgtttt gattcttcca ttaactcatc agcatcgatg 16321 gtgtggccct tatagttaaa ttcagcttta ctcatattgt ttctcctatc tataaattca 16381 gaatatgtta tcacttcatc agcaagtcca atctctattg ctttctcacc aaaatatagc 16441 tctgcttccg ttgatttgat tttttccgtg gaaagattcc tatttcttgc tacaagctcg 16501 acaaacatct catataggcg gtctacttca tcttgtaagc tttccac //