LOCUS KX181651 7190 bp DNA circular PHG 20-OCT-2016 DEFINITION Xanthomonas phage Xf109, complete genome. ACCESSION KX181651 VERSION KX181651.1 KEYWORDS . SOURCE Xanthomonas phage Xf109 ORGANISM Xanthomonas phage Xf109 Viruses; ssDNA viruses; Inoviridae; unclassified Inoviridae. REFERENCE 1 (bases 1 to 7190) AUTHORS Yeh,T.Y. TITLE Complete nucleotide sequence of a new filamentous phage, Xf109, which integrates its genome into the chromosomal DNA of Xanthomonas oryzae JOURNAL Arch. Virol. (2016) In press PUBMED 27743252 REMARK Publication Status: Available-Online prior to print REFERENCE 2 (bases 1 to 7190) AUTHORS Yeh,T.Y. TITLE Direct Submission JOURNAL Submitted (03-MAY-2016) Agricultural Biotechnology Laboratory, Plant Health Division, Auxergen Inc., 1100 Wicomico Street, Suite 700, Baltimore, MD 21230, USA COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..7190 /organism="Xanthomonas phage Xf109" /mol_type="genomic DNA" /db_xref="taxon:1852597" /lab_host="Xanthomonas oryzae ATCC 35933" gene 1..1041 /locus_tag="Xf109_1" CDS 1..1041 /locus_tag="Xf109_1" /note="Inoviridae phage pII protein; ORF1" /codon_start=1 /transl_table=11 /product="replication initiation protein" /protein_id="ANO58016.1" /translation="MSDGTCSFCGDPTAYFFPGGLCVACTSKNARIRMQEQPTQSREL SAFDASVGVMQAATRRTEIAAENIQKNKRVVGTSVREFDAAHPVALTAEGQRAALALG LVHYKTSDTRASATGTVTIEIDPLKARAQRLRKSVITGARLHDQEAKKGSFRGAWYFL TLTYRDGSDSSPGDVSELFKRMRGHFNRLKSGRARWNRESFRYVWVGELTQRFRPHYH VMLWVPTGMYFGKVDQRGWWPHGTTQIEKARNCVGYLAKYASKFTALTAGAFPKGFRT HGIGGLDTESKRELRWWKAPKDAREALGGEADIRKAKGGWFDRLTGEFWPSPWKVTFI FGRTFAWKVVQL" gene 1038..1334 /locus_tag="Xf109_2" CDS 1038..1334 /locus_tag="Xf109_2" /note="Inoviridae phage pV protein; ORF2" /codon_start=1 /transl_table=11 /product="ssDNA-binding protein" /protein_id="ANO58019.1" /translation="MKVQVMSSAVAVRSFPAREGKPATHFREQTAAVLRDGDFPLPFI IGLDEDQAPYGEGFYIIDPKSLQNNKFGGLEFGRRIRLIPDATAKAVQPAARVA" gene 1338..1541 /locus_tag="Xf109_3" CDS 1338..1541 /locus_tag="Xf109_3" /note="ORF3" /codon_start=1 /transl_table=11 /product="membrane protein" /protein_id="ANO58017.1" /translation="MAVLVPACLEADLDTAAGTCTAVIWIPQPSLLPELAVKDAQAIG SAIAFLWATAYVFRLIRKKIQQS" gene 1553..1783 /locus_tag="Xf109_4" CDS 1553..1783 /locus_tag="Xf109_4" /note="Inoviridae phage pVIII protein.; ORF4" /codon_start=1 /transl_table=11 /product="major coat protein" /protein_id="ANO58018.1" /translation="MRKLKTLFKNKTAALAAVGSAALVSAPAFASGGGGVDVGDVVSA IQGAAGPIASIGGAVLTVLVGIKVYKWVRRAM" sig_peptide 1553..1642 /locus_tag="Xf109_4" gene 1878..3326 /locus_tag="Xf109_5" CDS 1878..3326 /locus_tag="Xf109_5" /note="Inoviridae pIII protein; ORF5" /codon_start=1 /transl_table=11 /product="minor coat protein" /protein_id="ANO58020.1" /translation="MAGGLRDCLRGFRIMGWLARVFASAIARRLAYVLVAATLAWCGI GCASAQDFGTQGAAYSACMSQTAAYLASLGRGANDRNPECNVEQGSQAYRGQFETRDC SSCDWYRAYYGYFPWSKGCDQEPDYTGSGPWGTYVGTARNGSIGCRNGCDGVWFGNGD DTMTWNATGAICPKDPEKTCDAMSGGYGWNGYLGVCEPPPTEECPEGQVPDGKGGCSS NKCPEGMLLQADGTCAPKKNDCPAGQIRSPSGSCLPGDGQCAAGEVRGPDGTCKKDGD GDGEPDEPGEGDKSQFSGGDSCDSPPSCSGDAIMCGQARIQWRIDCNTRRDVNITGGS CASMPVCVGENCKALEYSQLLMQWRAACALEKAANNSGGGTGNNADVKAIRDAITGNG TADIGADGKPADAFSDESGYGEHGYPTGELDTHGFGYSRTCPTIPDVAVFGQTLHFDT SKFCQWMVLGGQIVLVMASLVSLRLMSQGGSA" gene 3326..3655 /locus_tag="Xf109_6" CDS 3326..3655 /locus_tag="Xf109_6" /note="ORF6" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ANO58021.1" /translation="MPWLIAQLVTALAWLFKSRIGLWIMTALVWLGINFGTIKMVVEP AIDLLKDYAQGMGSGNGQLGADAMAWFGVLQFDKALTMVISAVAAKHAIMQGRLFLFK RGFGAKP" gene 3655..4842 /locus_tag="Xf109_7" CDS 3655..4842 /locus_tag="Xf109_7" /note="Inoviridae phage pI protein; ORF7" /codon_start=1 /transl_table=11 /product="zonular occludens toxin" /protein_id="ANO58022.1" /translation="MPIELYTGQPGNGKTALMMERLVEEAKRAERPIFAAGIDGLQDG LATVLDDPRKWNDKDADDNYVVPNGSLIFVDEAWKWYGHLHDATRQQTPKHVLDLAEH RHRGLDFVWTTQQPNQLYPFVRGLIGAHTHVVRRFGTKMIDVFRWGELNEEIKSSAKR DLAQRTTRLLPSSIFGAYKSAEVHTIKPRIPWKVMALPGLVILAIALGWLAYTMLKPS AMAGGAKGTQSASADAAPGGSATTARRDGPRWESPTEYAKQHLPRFGTMPWTAPVFDD RSITADPMLICMSSLAGIDAQGKHKEASCTCMTEQGTAYDLDQPQCRTIAKRGPVYNP YRQQRQNEQQPAQQQQAAQGGAAAPGFNGIAVQRSTRTQGSFPESKAYSTKTTTPSTT LEM" gene 4844..5491 /locus_tag="Xf109_8" CDS 4844..5491 /locus_tag="Xf109_8" /note="Cf1c phage Cf1cp5 protein-like; ORF8" /codon_start=1 /transl_table=11 /product="conjugal transfer protein TrbP" /protein_id="ANO58023.1" /translation="MTSSGREALKWIALVLMTGDHVAKVLFGGYVPVLSELGRIAFPV FALVMAYNLAQPRADYAKSVLRLAGWGLLAQPFHAWAFGYWLPLNVLLTFACAALLVW TVHARHWLYAFVFAVVVPVLVDYQWFGVLLVLAAWGLFRSGRLQWLVGVLASMAALCW YNGNVWALAALPVLALGYVWWPLPRLRWAFYGYYVGHLGLLVLIVSLPAFRQHVA" gene complement(5663..6046) /locus_tag="Xf109_9" CDS complement(5663..6046) /locus_tag="Xf109_9" /note="DUF3653 protein; Cf1c phage Cf1cp7-like and phi-Lf phage ORF110-like protein; ORF9" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ANO58024.1" /translation="MTFDTYERVDLTGPWAGFGFQGHRFFTPENYDIDPCGMRYWALT CAIAREWSLMMSEERNAQSANPRTPTATRSPGSRISEDANVIYLRDVLWRRREKRLSV GGNPGLADSGKRSPTGRGRRRPRRG" gene complement(6043..6513) /locus_tag="Xf109_10" CDS complement(6043..6513) /locus_tag="Xf109_10" /note="Cf1cp8-like protein; ORF10" /codon_start=1 /transl_table=11 /product="helix-turn-helix XRE-family DNA binding protein" /protein_id="ANO58025.1" /translation="MSAENELIDLVRAGGKFSSDNALAQKLGVTRAMVSSWRSGRYAM PDDQIAQLCALAKLDGASWMARIHTERAGSATERALWKSILDRLAPITAVVGALAIVA VGLHAGAHEGLLTALSPLAITPTVYTLCEVVLLTLLCTVAAYYCRSLHRKRTGQ" misc_recomb complement(6121..6135) /note="phage attachment site; attP" gene 6641..6826 /locus_tag="Xf109_11" CDS 6641..6826 /locus_tag="Xf109_11" /note="ORF11" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ANO58026.1" /translation="MGFQVQYRDGRRWVVLASHPTRNAARDDAAARIAFLVADGFKYG DVVRDFRVRGVTVAEAA" gene 6826..7038 /locus_tag="Xf109_12" CDS 6826..7038 /locus_tag="Xf109_12" /note="ORF12" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ANO58027.1" /translation="MALSTDTNALALIGASALTVIVGLARLVAWILDRRAEAALRAHR EQVFIIESYVSLVAPTVSRRVTICSA" ORIGIN 1 atgtctgacg gcacatgctc cttctgcggc gaccccactg cctatttttt tccaggcggt 61 ttgtgtgttg catgcacctc gaaaaacgca cgtatacgca tgcaggagca gcccacgcaa 121 tcgcgtgagc tttctgcgtt tgatgcatct gtcggcgtca tgcaggccgc tacgcgccgc 181 actgaaattg ctgcagagaa tatccaaaag aacaagcgtg tggtcggtac aagcgtgcgt 241 gagttcgacg cggcgcatcc ggtcgcattg accgcagagg gccagcgcgc agcgctggcc 301 cttgggcttg tccattacaa aacaagtgac acgcgcgcct ctgcgacagg caccgtgacc 361 atcgaaattg acccgctgaa agcgcgggcg caacggctgc gaaagtccgt gattaccgga 421 gcacgtctgc atgatcagga agcgaaaaaa ggttccttcc ggggtgcgtg gtatttcctc 481 acgctcacct accgtgatgg aagcgacagc agccctggtg acgttagcga actatttaaa 541 cgcatgcgcg gccacttcaa tcgccttaaa tctgggcgcg cacggtggaa ccgtgaaagc 601 tttcgttacg tatgggtcgg agaactcacc cagcgattcc gcccgcacta tcacgtgatg 661 ctttgggttc ccactggtat gtatttcggc aaggttgatc aacgcggctg gtggccacat 721 ggcacaacgc aaattgagaa agcccgcaac tgcgtcggct atctcgcgaa atacgcgagc 781 aagttcactg cccttacagc tggagctttt cccaaaggct tccgcaccca cgggattggt 841 ggactcgata ccgaatctaa gcgcgagttg cgctggtgga aagccccgaa agatgcgcgt 901 gaagctctcg gcggggaagc ggatatccgc aaagcaaagg gcggatggtt cgacaggctt 961 accggggagt tctggccgtc tccgtggaaa gtcacattca ttttcggccg gacattcgcc 1021 tggaaggtag tccaactatg aaagtccaag tcatgagttc cgctgtcgct gttcgttcct 1081 ttcctgcgcg tgagggtaag cccgcgacgc atttccgtga gcagaccgca gctgtgctgc 1141 gcgatggaga tttcccgttg cctttcatca tcggtctcga tgaggatcaa gcgccctacg 1201 gtgaaggttt ttacatcatc gatcccaagt cgttgcagaa caacaaattc ggtggtctcg 1261 aattcggtcg tcgtatccgc ctgatccctg atgccactgc caaagctgta cagcctgcgg 1321 cgcgggtcgc ctaacccatg gccgtgctcg ttcccgcatg cctggaagcc gatttggaca 1381 cggcagcggg gacgtgcacg gcagtgatat ggattcctca accgtcactc ttgccggaac 1441 tggcggtgaa ggatgcccag gccattggca gcgcaatcgc gttcctgtgg gccacggcgt 1501 atgtgttccg gctcatccgc aagaaaattc aacagtccta ggaggacatg caatgcgcaa 1561 gctcaagacc ctgttcaaga acaagaccgc tgctctggcc gcagttggtt cggccgctct 1621 cgtctccgct ccggcgttcg cgtccggcgg tggtggtgtg gatgtgggcg atgttgtgtc 1681 tgccatccag ggtgccgcag gccccattgc ctccattggc ggtgcagtgc tgaccgtcct 1741 ggtgggcatc aaggtctaca agtgggtgcg ccgcgccatg taacgaccac cggcggacag 1801 ggccaactcc ctcccgccgg tcttttacgg ggatagggca ggggaacggc gatggaaggg 1861 tggatttggt tcggcgcatg gctggtggcc tgcgcgattg tcttcgtgga tttcgaataa 1921 tgggttggct cgcacgcgtg tttgcatccg cgattgcgcg tcgcctcgcc tacgtgcttg 1981 tagcggcaac gctcgcatgg tgtggcattg gttgtgccag tgcgcaagac ttcggaacgc 2041 aaggcgctgc gtattccgca tgtatgtcgc aaaccgctgc ctatctagcc tcccttggcc 2101 gtggtgctaa cgacaggaat cccgagtgca atgtcgagca gggcagtcaa gcgtatcgcg 2161 gccaatttga aacaagggac tgtagtagtt gtgattggta tcgggcttac tacggttatt 2221 tcccgtggtc taagggttgc gatcaggagc ctgattacac tggttctggc ccatggggta 2281 cgtatgtggg tactgcgcgc aatggcagca tcggttgccg caatggatgc gacggtgttt 2341 ggttcggcaa tggcgatgac acgatgactt ggaacgccac gggcgcaatc tgtccgaagg 2401 atccagagaa gacttgtgat gccatgagtg gtggttatgg ttggaatggt tacctgggcg 2461 tatgtgagcc gccgccgacc gaagagtgtc cggaagggca ggtgcctgac ggcaagggcg 2521 ggtgttcgtc gaataagtgt cccgaaggca tgttgcttca ggctgacggc acatgcgcac 2581 cgaagaagaa cgattgccct gctggtcaga tcaggtcgcc cagcggctca tgtctccccg 2641 gcgatggtca atgcgctgct ggtgaagtgc gtggtcccga tggcacgtgt aaaaaagatg 2701 gtgatggcga tggcgagcct gacgagccgg gggagggcga caagagccag ttttctggcg 2761 gtgatagctg cgattcgccg ccgagttgta gcggtgacgc gatcatgtgc ggacaggcgc 2821 ggattcaatg gcgcattgat tgcaacacac gccgcgacgt aaacatcaca ggcggttcgt 2881 gtgcatcgat gcctgtgtgc gtcggcgaga actgcaaggc ccttgaatac tcgcaattgt 2941 tgatgcaatg gcgggctgcg tgtgcgctgg aaaaggcggc gaacaacagc ggcggcggta 3001 cgggtaacaa tgcagatgtt aaggcgattc gcgatgcgat cactggtaat ggcacggctg 3061 atatcggtgc cgatggcaag cctgcggacg cgttttccga tgagtcggga tatggcgaac 3121 acggttaccc aacgggtgaa ctcgatacgc acggtttcgg ctacagccgc acgtgtccaa 3181 cgattcccga tgttgcggtg ttcggccaaa ccctgcactt cgacacgtcc aagttttgcc 3241 agtggatggt gcttggcggc caaattgtgt tggtcatggc atccctggtt tccctacgtc 3301 tgatgagtca aggaggtagc gcataatgcc ctggttaatc gcacaactcg tcaccgcgct 3361 ggcatggctg ttcaagtcgc gtatcggtct atggatcatg accgcgctgg tgtggctcgg 3421 catcaatttc ggcaccatca aaatggtcgt tgagcctgcc atcgatctgc tcaaggacta 3481 cgcccaaggc atgggcagcg gcaacggcca actcggtgca gacgcgatgg catggtttgg 3541 cgtgcttcag ttcgacaagg cactgaccat ggttatctct gcggttgcag ccaagcacgc 3601 catcatgcaa gggcggctgt tcctgtttaa gcgtggattc ggagccaagc cgtaatgcca 3661 atcgagctat ataccgggca acccggcaat ggtaaaaccg cgctcatgat ggagcgcctg 3721 gtcgaagagg ccaagcgcgc cgagcggccg attttcgctg caggaatcga tggattgcag 3781 gacggtctag cgaccgtgct ggacgatccc cgcaagtgga acgacaagga tgcagatgac 3841 aactatgtcg tgccgaatgg ctcgttgatc ttcgtagacg aagcatggaa gtggtatggc 3901 catttgcacg acgccacgcg ccagcagacg cccaaacacg tgcttgacct tgctgagcat 3961 cggcatcgcg gacttgactt tgtgtggacg acgcagcagc cgaatcagct gtacccgttc 4021 gtgcgtggat tgatcggcgc acacacccac gtggtgcgtc gtttcggcac caagatgatc 4081 gacgtgttcc gctggggcga gttgaacgaa gaaatcaaat cgtctgctaa acgcgatctt 4141 gcccagcgca ccacgcgtct gctgccttcc tcgatcttcg gcgcatacaa gtcggctgag 4201 gtgcacacga tcaagccgcg cattccgtgg aaagtgatgg cgttgccggg tttggtcatc 4261 cttgccatcg cgcttggatg gctcgcctac acgatgctca agcccagcgc gatggccggc 4321 ggagctaagg ggacgcaatc ggcgtcagcc gatgcggccc ctggcgggtc tgcgaccacc 4381 gcacggcgtg atggtccgcg ttgggaatct cccaccgaat atgccaagca acaccttccc 4441 cggttcggca ccatgccgtg gacagcaccg gtgtttgatg atcgcagcat cactgccgat 4501 ccgatgttga tctgcatgtc gtcgctcgct ggcatcgatg cgcagggtaa gcacaaagaa 4561 gcgtcctgca cgtgtatgac agagcagggc acggcgtacg acctcgacca gccgcagtgc 4621 cgcacgattg ccaagcgcgg cccggtctac aacccgtatc gtcagcagcg ccagaacgag 4681 cagcagcccg cccagcagca acaggcggcg cagggtgggg cggctgcgcc tgggttcaat 4741 ggcatcgctg tgcagcgctc tacgcgcaca cagggtagct ttcctgagtc gaaggcgtac 4801 agcaccaaga ccaccacgcc ttccaccacg ctggagatgt gacatgacca gcagcggccg 4861 cgaggcgttg aagtggattg cgttggtgtt gatgaccggc gatcacgtgg cgaaggtgtt 4921 gttcggcggc tatgtgccgg tcttatccga attggggcgg atcgcgttcc cggtgttcgc 4981 gttggttatg gcgtacaacc tcgcccagcc acgggccgac tacgcgaaat cggtgctgcg 5041 ccttgccggc tgggggctgc tggcgcagcc attccacgcc tgggcattcg gctactggct 5101 gccgctcaac gtgctgttga cgttcgcatg tgctgcgctg ctggtgtgga ctgtccacgc 5161 acggcactgg ctctatgcgt tcgtgttcgc agtggtggtc ccggtgctgg ttgactatca 5221 gtggttcggc gttttgctgg tcctggcagc atggggcttg ttccgcagcg gccgcctgca 5281 atggctcgtc ggagtcctgg ccagcatggc tgcgctgtgt tggtataacg gcaacgtgtg 5341 ggcgctggcg gctctgcctg ttctggcgct tgggtacgtg tggtggccac tccctcggct 5401 gcgctgggct ttctacggtt attacgttgg ccacctcggc ctgttggtgc tcattgtgtc 5461 gctgccggcg tttcggcagc acgtcgcgta gatagatgac gttctcggaa aacaaggcct 5521 acaccacgaa caccacgacg ccgcctacga cctttcgttg acagcccctg cggggcgcag 5581 tcacaggggc gcggcttttc gcctggatct gtcagttcgc atgtagccgg ggtgtagggg 5641 cagcgcccct acggataacg cctcacccgc gccgtggacg ccgccgcccc cgtccagtcg 5701 ggctgcgctt gccgctgtcg gctaaccccg gattcccccc cactgataac cgcttttcac 5761 gcctgcgcca gaggacgtcc cgcaggtaga tcacgttggc gtcttcagaa atccgcgacc 5821 ccggagacct tgtggcagta ggcgttcgcg ggttcgccga ttgcgcattg cgttcttcgg 5881 acatcatcag cgaccactcc cgtgcgatgg cacacgtcag cgcccagtac cgcatgccgc 5941 aagggtcgat gtcgtaattt tctggtgtga agaatcgatg tccctgaaaa ccaaaaccgg 6001 cccaagggcc ggtcaggtct acgcgttcgt aggtgtcaaa cgtcattgtc cagtccgctt 6061 cctgtggagg gaccggcagt aataggctgc gacggtgcaa agcagcgtca acagtacgac 6121 ttcgcataat gtatagacgg ttggcgttat ggcgagcggg gaaagggccg tcagcagccc 6181 ctcatgagca cctgcgtgca gcccgactgc cacgattgcc agcgccccga ccactgcggt 6241 gatcggggcc agccggtcca ggattgactt ccacagggcg cgctctgttg ccgatccagc 6301 acgttcagtg tgaatccttg ccatccaact ggctccgtcc agcttcgcta gcgcacaaag 6361 ctgcgcaatt tggtcgtccg gcatcgcata tctgcccgac cgccacgagc tcaccattgc 6421 cctggtgacg cccagcttct gagctaaggc attgtctgaa ctgaattttc ctcctgctcg 6481 caccaggtcg atcagttcgt tttcggcgct catgtttagc tgacctttac attcttgttt 6541 cgttgaacta tacaagcatc agcgtttagc gcggctaagc gccccgcccc ccggtacccc 6601 ccgggggtca cgggtcaggg taggggatag gggcttcacc atgggatttc aggttcagta 6661 ccgcgacggt cgccgctggg tggtcctcgc ttcgcatccg acccgtaatg cagcgcgtga 6721 cgatgccgct gctcgcattg cctttttggt cgccgatggt ttcaagtatg gcgatgtagt 6781 ccgcgatttc cgcgttcgtg gtgtcaccgt cgcggaggcc gcctaatggc actgtcgact 6841 gacaccaacg cacttgcatt gatcggcgct tccgcgctga ccgtgatcgt cggcctcgcc 6901 cggttggtcg cctggattct tgaccgtcgc gccgaagctg cgctgcgcgc acaccgcgaa 6961 caggtcttca tcatcgaaag ctacgtttcc ctggtcgcgc cgactgtttc gcgacgcgtc 7021 acgatttgta gcgcctaacg cgaggtgtgc cgtggttgat tttcttgttt tcgagtgcga 7081 gcggtgcggc cgttttgatc gtgcggtctt tgcccattgc actgaggaag gtgttttgtg 7141 cgacgactgc tttgatgtcc tgttcgattc gctctctgag gatgagagcg //