LOCUS MF417857 10286 bp DNA linear ENV 27-FEB-2018 DEFINITION Uncultured Caudovirales phage clone 8S_4, partial genome. ACCESSION MF417857 VERSION MF417857.1 KEYWORDS ENV. SOURCE uncultured Caudovirales phage ORGANISM uncultured Caudovirales phage Viruses; dsDNA viruses, no RNA stage; Caudovirales; environmental samples. REFERENCE 1 (bases 1 to 10286) AUTHORS van Zyl,L.J., Abrahams,Y., Stander,E.A., Kirby,B.M., Clavaud,C., Farcet,C., Breton,L. and Trindade,M.I. TITLE Novel phages from South African skin metaviromes JOURNAL Unpublished REFERENCE 2 (bases 1 to 10286) AUTHORS van Zyl,L.J., Abrahams,Y., Stander,E.A., Kirby,B.M., Clavaud,C., Farcet,C., Breton,L. and Trindade,M.I. TITLE Direct Submission JOURNAL Submitted (29-JUN-2017) Biotechnology, University of the Western Cape, IMBM, C2, Life Science Building, Robert Sobukwe Rd, Cape Town, Western Cape 7550, South Africa FEATURES Location/Qualifiers source 1..10286 /organism="uncultured Caudovirales phage" /mol_type="genomic DNA" /isolation_source="healthy skin from scalp of human male" /db_xref="taxon:2100421" /clone="8S_4" /environmental_sample gene complement(<1..475) /locus_tag="8S4_1" CDS complement(<1..475) /locus_tag="8S4_1" /note="Predicted by MGA" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN67590.1" /translation="MTPAQIIQAIEGMEPAMQRAYLEQVKTVVGAATVAEVERLIAEE DEEGLVSLLSLGALSVFLELARSVFIAGAKFEVKAIMIPRELGRFEFDARQPAAEKWV SAKAEEIRANASIDVRAAIREVMGSRRRVVGWPSAQPAAVQVEVSATPMIRTPRQA" gene complement(472..1029) /locus_tag="8S4_2" CDS complement(472..1029) /locus_tag="8S4_2" /note="Similar to YP_009043597.1 [Pseudomonas phage phiPSA1] (BLASTp score : 123). Similar to PB008624 [Pfam-B_8624] (hmmscan score : 144.8)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN67591.1" /translation="MPLIVEDGTGLPAAESYISLDSLKAYAAGRGVDISGVTDEQNES ALRRATTYIDGKYRKHLQSGRWASLFSGEKKNGRGQALEWPRSGAEDYEGLPISDSTV PIEVKNATAEAGYREALNPGSLSPDFVSASMVKREKVGPLETEFAVSVGADAAGSVRP VISIIDEMIAPVLVARYTLPAVFTV" gene complement(1812..2198) /locus_tag="8S4_3" CDS complement(1812..2198) /locus_tag="8S4_3" /note="Predicted by MGA" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN67592.1" /translation="MKVTNNSKALQGVHTTAGVVYILPGQTQDIDLTPEGHKGASRLA FMSVEGKAPTGDSDERAELFAKLKALGIDAAGNSRTETLQKKLDEALAAAEKQKVMDE LTALNVEFDKEANLEALQAALAAAKA" gene complement(2211..3173) /locus_tag="8S4_4" CDS complement(2211..3173) /locus_tag="8S4_4" /note="Similar to YP_009043596.1 [Pseudomonas phage phiPSA1] (BLASTp score : 350). Similar to PF09950.4 [DUF2184] (hmmscan score : 220.9)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN67593.1" /translation="MPQVFEDAQSAFPFVLAQGRNIETRIYTRRYPTFNYAASIPVVT EGAPWAIGTTFFTVDTAGEAKFLSGSGTDMPFNSATHDQASHDFAMIGSGWEWNLEEV NQAQLYGINLSGTKADSAADKVERLLNSIAFVGSTEKRWTGLLNDGNVSRVDAAASGT AGSTYWSAKDVDLIMADVNGLLGGIRTNTAEVEWADTLRMPPDAFRAVATKRMGAGDG FMTVLEYMRRNNIYTAETGLPLDIAPLREARNASADGGGRLVAYRKDPEVVRFHLPMP RRVLAPRQKSIMGFETGIIARTGGTEIRLPGAVAYLDEITPPAA" gene complement(3175..3654) /locus_tag="8S4_5" CDS complement(3175..3654) /locus_tag="8S4_5" /note="Similar to PB009272 [Pfam-B_9272] (hmmscan score : 76.9)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN67594.1" /translation="MATYQTTYTNAPAKGVPGLVANEEKCNKISRTVSNAEGIVFGAP GFRVAGAGNDHKIAATGTLFLGLAVLSAAVPPVATGSTLIDGYPQDFTGAFMTDGQMY VTAGAAVVPGDDVYYVAATNRYVTTAAAGAVLIPGAFFDTTGANGDIVEISLKHRSA" gene complement(3665..4795) /locus_tag="8S4_6" CDS complement(3665..4795) /locus_tag="8S4_6" /note="Similar to YP_009043595.1 [Pseudomonas phage phiPSA1] (BLASTp score : 523). Similar to PF09979.4 [DUF2213] (hmmscan score : 134.6)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN67595.1" /translation="MIFTDSVPVTGVRRTEDGYLVAEARVARTGIQDYLGTEIDPDNE HGLRDKPIVRVYRPESAVFHADAMHSYAYRPMTNGHPGGNGVNSKNWKDVAIGQTGGE VVRDGQFVKVPLVLMDAKAIEDYESGKRELSMGYGAEVVFQDGVTGDGEQYDCYLGPM KMNHLSLEHRARGGIDLRIGDHKPDTPKGGHDMADALRKLLVDGISIDVTEQGAQAIE KLNTKLADAATATKTLTDAHATAIALKDSELAKKDAEIDALKAKQLSDADIDKRVTAR ADLLTKAKTIADADYTGKTDAEIRKAVVVAKLGDAAVAGKADAYIDARFEILVEDAAK DPAVDPFRKHMIQQDGKTVGDESEKARLQMIVDMQTAHLPKA" gene complement(4792..6597) /locus_tag="8S4_7" CDS complement(4792..6597) /locus_tag="8S4_7" /note="Similar to YP_009043594.1 [Pseudomonas phage phiPSA1] (BLASTp score : 1013). Similar to PF06381.6 [DUF1073] (hmmscan score : 332.1)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN67596.1" /translation="MSAFSYLKDSLQNLVAGLGTARDKASHSHYAIPEMDDQQLLNAF RGSWTAQKGVTIPAVDACRNWRSWQADKGQIELIEAEEDRLNVKGKILEALLKARLFG GAAVFIGTGERDTASELNPERVRQGGVKYLTVMTRRQLSATEIEQDPQSPRFGKPKAY RLPGSVVEIHPSRLVIFIGTPHPDPELAVGCGFGWGDSVLLAAMPAVRHYDETVANVV SLVYEAKIDVINIPNLMSSMQDKNYERLLLERLRLAATAKGINGTLILDGAETHSSKS ASFGNLPEVIAKTEQGVSGAFDIPGTRMFGQSSTGLGANGEENTRNYYDNVASRQKLE IKPAMSVLDECLIRSALGVRPKEIHYAWAPLWQATAKERADIGKTTADTLKALKDSGL FPEEALSAASVNLLVELSVMPGLEAAIDQFGAELPDEEGGAADDDLPVSEGEPVAGKK DLSDAAPRTLYVSRKVTNGAEIVAWAKAQGFESTLPAEDLHVTVAYSRSPVDWMKVGE SWSGDGKGQLKIAPGGARLIDKFGEGAVVVLFNSSELAWRHVTIVEAGASWDWPDYQP HITFTYEPGTVDIDKVEPYRGAIELGPEIFEELDP" gene complement(6594..7874) /locus_tag="8S4_8" CDS complement(6594..7874) /locus_tag="8S4_8" /note="Similar to YP_009043593.1 [Pseudomonas phage phiPSA1] (BLASTp score : 802). Similar to PF03237.10 [Terminase_6] (hmmscan score : 123.8)." /codon_start=1 /transl_table=11 /product="putative terminase" /protein_id="ASN67597.1" /translation="MQAVLMLNKPQFEFIKSHKKFMAFVGGYRSGKTFVGCVRMCVNA LEFPGIPQGYFAPTYPQIADIFYDTMPVVAEAFGLFADIVPSKKRVYLRDSRGRCLST IVCKSMEHPHRIVGFNIAHALVDEIDCMPIKKADSAWKKIIARMSTVWPGRDMNTIDV TTTPEGFNWVYRKFVKELASNPSQRPLYGIVHASTRQNAKNLPKDYIKSLRESYPANL VDAYIDGLFVNLTSGSVYPSFCRKQNHTDATIRPGEQLHVGMDFNINRMAATIHVIRD GLPMLLEEATSLFDTPAMIVELKRRFPGHSITVYPDASGKNRKSVNGSESDHSLLRAA GFMVMVNPSNPIVRDRVLAVNAMFLNIDHKRRYLVNTDNCPVTTQVLEQQAYSENGEP NKDGTEDPVDALGYFIVQRFPIAGSYTLANVSNQ" gene complement(7849..8418) /locus_tag="8S4_9" CDS complement(7849..8418) /locus_tag="8S4_9" /note="Similar to PB016974 [Pfam-B_16974] (hmmscan score : 70.1)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN67598.1" /translation="MALCGASKRGNGEPCKRHAIPGSSRCKLHGGKSSGPKEQRGNKN AAKPGSIYSRFLTDEENDMLASIELGRVDDELRLTRVRLMRALARESEFGNTLEVESE KEEPILINGKETSLTSITTTSKVRDYSSLIDRLTARVESLERTKEDLETRRLTNEKLR RELEDPNKGLPEPKQVIIGVEDASCPDAE" gene complement(8489..8704) /locus_tag="8S4_10" CDS complement(8489..8704) /locus_tag="8S4_10" /note="Predicted by MGA" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN67599.1" /translation="MSDQSGEHVHHWDDGRGRREVIIDGQPVTHVLWCDAMAGIAVVA DQPIKSSNGERVDSHPVWGEVKVLSIE" gene complement(8701..8910) /locus_tag="8S4_11" CDS complement(8701..8910) /locus_tag="8S4_11" /note="Predicted by MGA" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN67600.1" /translation="MKVIVTKLLGSAEVEYLRQGVVVHRERFTGKVTSEYRRTIAFNE AFDTHRCRFVTAMPADRAFQYEVAP" gene complement(8960..9292) /locus_tag="8S4_12" CDS complement(8960..9292) /locus_tag="8S4_12" /note="Similar to YP_001293404.1 [Pseudomonas phage F10] (BLASTp score : 120). Similar to PF05106.7 [Phage_holin_3] (hmmscan score : 121.5)." /codon_start=1 /transl_table=11 /product="putative holin" /protein_id="ASN67601.1" /translation="MSNMPDKPDTWAIALAWLSQHSPILYAAALSCAMAVLRITYGGG TRRQMIVEGAICGGLALTIISGLEFFSLPQSMATFVGGWVGFLGVEKIRSIADRVTDF KLPGRKVD" gene complement(9442..9819) /locus_tag="8S4_13" CDS complement(9442..9819) /locus_tag="8S4_13" /note="Predicted by MGA" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN67602.1" /translation="MSCSIQSSNYVPGVSGWKLDQATGTFEINGLPRERSNTVIKDGK LVISTNGAPQVVLGNLPDLRDAQELKPFIVVDGVTYIRQGFIDDATVTKALITQECSA RITADSVMSARISALEAQISQLR" gene complement(10076..10240) /locus_tag="8S4_14" CDS complement(10076..10240) /locus_tag="8S4_14" /note="Predicted by MGA" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN67603.1" /translation="MKLKTRINTLERARTAWEAVARQVGETDFSRHPRTGEYLHPGVA MGWRIHKKNL" ORIGIN 1 ctgcctggcg aggcgtgcga atcatcggcg tagcgctgac ttccacctgc actgcagcag 61 gctgtgcact gggccacccg accacgcgcc ggcgactacc catcacttcg cgaatagctg 121 cgcgcacatc gatggatgcg ttggcgcgga tctcttcggc cttggccgac acccacttct 181 cagcagcggg ctgcctggca tcgaactcaa aacgtccaag ctcacggggg atcatgatcg 241 cctttacctc gaacttggcg ccggcgatga acaccgaccg cgccagctca aggaacacgg 301 acagcgctcc caggctgagc agcgatacca ggccctcttc atcctcttcc gcgatcaggc 361 gctcaacttc tgcaaccgtt gccgcgccga ccaccgtctt gacctgctcc agataggccc 421 gctgcatcgc aggctccatc ccttcgatgg cctggatgat ctgcgccggg gtcataccgt 481 aaacaccgca ggcagcgtgt agcgagccac cagaacaggg gcaatcatct cgtcaatgat 541 gcttatcacc gggcggacag atccggcggc atctgcaccc acggaaacgg cgaattcagt 601 ttccagcggg ccgaccttct cccgcttgac catcgaggcg gacacgaagt cagggctgag 661 gctgccgggg ttaagggcct ccctataacc agcttcagcg gttgcgtttt tcacttcgat 721 cggcactgta gagtcggata tcggaaggcc ttcgtaatct tcagcgccac tccttggcca 781 ctccagcgcc tgaccacggc cattcttttt ctctccagag aaaagcgatg cccacctacc 841 agactgcaga tgtttcctat atttgccatc gatgtaggtg gttgccctgc gaagcgcgga 901 ctcattttgc tcatctgtaa ccccagatat atctacgccg cgccctgctg cataagcctt 961 cagcgaatcc agtgaaatat aggactcagc ggcaggaagt ccggtgccgt cctcaacgat 1021 aagtggcata atcaattccc ggagttattt ctatgtggcg ccaaagcgca ggagacgttt 1081 atgagtagct gtagtgttga tggctgtgcc aagccgcatc aggctcgcgg ctactgcgaa 1141 agccattatc gaaagctctt aaaatatggc gacgcagagt acgtaaagcc tatgtttgtg 1201 cctgatcgct gcaaagctga tgcgtgcaag cgaaatgcgg agtgccaagg ctattgcaaa 1261 gcgcattacg cgcgcttact caggcatggc gatccattgc aggggcgaac tgcaatcgga 1321 ggcgcgctta attggcttga atccaatgcg tcacacacag gcgacgagtg cctaatatgg 1381 cccttcaggc gccggcctaa cggctatggc gaagcaacct tccgaggcgg gcgcatggct 1441 gcaaaccgga taatgtgcat tcttgctcat ggcgagccgc cgactgaaga acatcacgca 1501 gctcatgact gcggcaatgg ttttggcggc tgcgtaaatc ccagacacct tcggtgggct 1561 accaagtctt ccaatgaaat ggacaaggtt gatcacggca catctaatcg cggctcgcgt 1621 cacggcatgt ccaagctgac agagagcgaa gtgcgctcaa tccgtgcagc agagggatcc 1681 atgacccaaa aggctatcgc cgcgcaattt gggatcagca gagcaactgt tggtagcatc 1741 caatcaagac agcgttggtc ttggctggac tgaataggtg gagcgacatg cgctccgggt 1801 tttgcggggt gttacgcctt ggcggctgcc agtgcggcct gcagggcttc caggttcgct 1861 tccttgtcga actcgacgtt cagcgcggtc agctcatcca tgaccttctg cttttcggcg 1921 gcggccaggg cttcgtccag cttcttctgc agggtttcgg tcctgctgtt gccagcagca 1981 tcgatgccca gcgccttcag cttggcgaac agctcagcgc gctcgtcgct gtcaccggtc 2041 ggcgctttgc cctcgacact catgaaggcc aggcgcgagg cgcccttgtg cccctctgga 2101 gtgaggtcaa tgtcctgcgt ttgtcccggc aggatgtaga cgactccggc cgtggtgtgc 2161 acgccctgca gcgccttcga gttattggtc actttcatgt cgacctccta tcaggctgct 2221 ggtggggtga tttcgtcgag gtacgccacg gcgccaggga gacgaatctc ggtaccgcca 2281 gtacgcgcga tgattccggt ctcgaagccc atgatggact tctggcgcgg ggccaggacg 2341 cggcgcggca tcggcagatg gaagcgaaca acttcagggt ctttgcggta cgcaaccagg 2401 cgaccaccac cgtcagcgga agcgttgcgg gcctcgcgca gcggcgcgat gtccagcggc 2461 agaccggttt cggcggtgta gatgttgttg cggcgcatgt attccagcac cgtcatgaag 2521 ccatcgccag cacccatgcg tttggttgcc acggctcgga atgcatcagg cggcatgcgc 2581 agggtgtctg cccactcgac ttcggcagtg ttggtacgga tgccaccaag caagccgttc 2641 acgtcggcca tgatcaggtc gacgtctttc gctgaccagt aggtcgaacc agcagtgccg 2701 gacgcagcag cgtcaacgcg cgaaacgttg ccgtcgttca gcaggccagt ccaacgcttc 2761 tcggtgctgc ctacgaacgc gatgctgttc agcaggcgct ctaccttgtc ggcggcggaa 2821 tccgccttgg tgccactgag gttgataccg tacagctggg cctggttcac ttcttccaag 2881 ttccattccc agccggagcc gatcatggcg aagtcgtgcg atgcctggtc atgggtggcc 2941 gagttgaacg gcatatcagt accagaaccc gacaggaact tggcctcacc ggcagtatcg 3001 acagtgaaga aggtggtgcc gatggcccat ggagcgcctt cagtgactac tgggatgctc 3061 gcggcgtagt tgaacgtcgg gtagcggcgc gtgtagatgc gcgtttcgat gttccggccc 3121 tgggccagaa cgaacgggaa cgccgactga gcgtcttcga aaacttgagg catgttaagc 3181 gctccgatgt ttgagggaga tttcgacgat gtcaccgttc gcaccggtgg tgtcgaagaa 3241 ggcgccaggg atcagcacgg caccagcggc ggcagtcgtc acgtaacggt tggtggcggc 3301 gacgtagtaa acatcatcgc ccggcactac cgcggcgccg gcggtgacat acatctggcc 3361 gtcggtcatg aacgcgccgg tgaagtcttg cgggtagccg tcgatcaggg tggagccggt 3421 agccacgggc ggcacggctg cgctcagaac tgccagaccg aggaacaggg tgccagtggc 3481 ggcgatcttg tgatcgttgc cagcaccagc cacacggaag ccaggcgcgc cgaagacaat 3541 gccttcagcg ttcgagacgg tgcgactgat cttgttgcac ttctcttcgt tggcgaccag 3601 gccgggcacg cccttggctg gagcgttggt gtacgtggtt tggtaagtag ccatggtgtg 3661 ctccttatgc cttcggcagg tgggcggttt gcatgtcgac aatcatctgc agacgtgctt 3721 tttccgattc gtcgccgacg gtcttgccgt cctgctgaat catgtgtttg cggaaggggt 3781 cgacggccgg gtctttcgcg gcgtcttcca ccaggatttc gaagcgcgca tcgatgtagg 3841 catcagcctt accggctacg gccgcgtcac ccagcttggc gaccacgacg gccttgcgga 3901 tttcggcgtc ggtcttgccg gtgtagtctg catcggcgat ggttttggcc ttggtgagca 3961 agtcggcacg tgctgtcacg cgcttgtcga tgtcggcatc gctgagttgc ttggccttga 4021 gggcatcgat ctcggcgtct ttcttcgcca gttcgctgtc cttcagcgcg atcgcagtag 4081 cgtgcgcgtc ggtcagggtc ttggtggcgg tagcagcatc ggcaagcttg gtgttcagct 4141 tctcgatggc ctgggcgcct tgctcggtga catcaatgga gatgccatcg acaaggagtt 4201 ttcgcagtgc atcagccatg tcatggcctc ctttgggggt gtctggtttg tggtcaccga 4261 tccgtagatc gatgccgccc cgagcgcgat gctcaaggct gaggtgattc attttcattg 4321 ggcccaggta gcagtcgtac tgctcgccgt cgccggttac gccatcctga aagacgacct 4381 cggcgccgta gcccatggag agctcacgct tgcctgactc gtagtcctcg atggctttgg 4441 cgtccatcag caccagggga accttgacga actgaccgtc gcggaccacc tcgccacctg 4501 tttggccaat cgcgacgtcc ttccagttct tggagttgac gccattgccg cccgggtggc 4561 cgttggtcat aggccggtat gcgtacgagt gcatcgcgtc ggcgtggaat accgcgcttt 4621 caggccggta cacgcgcaca attggcttat cgcgcaggcc gtgttcgttg tccgggtcaa 4681 tctcggtgcc caggtagtcc tgaatgccgg tgcgcgctac ccgcgcctcg gctaccaagt 4741 aaccgtcctc ggtgcgccgc acgcccgtga ctggcacgga gtcggtgaag atcatgggtc 4801 gagttcctcg aagatctcgg gccccagctc aatcgcgcca cggtatggct cgaccttgtc 4861 gatatcgacg gtgccgggtt cgtaggtgaa agtgatgtgt ggttggtagt ccggccaatc 4921 ccaagacgcg cccgcctcaa cgatggtgac gtgacgccag gccagctcgg aactgttgaa 4981 cagcaccacc acagcaccct ccccgaactt gtcgatgagc cgggcaccac caggtgcgat 5041 cttcagctgg cctttcccgt caccggacca ggattcaccg accttcatcc aatcaaccgg 5101 tgaacggctg taggcgacgg tgacatgcag gtcttccgct ggaagcgtcg actcgaaccc 5161 ttgagccttg gcccaagcga cgatctcggc accgttcgtg accttgcggg acacgtacag 5221 cgtgcgcggt gcggcgtctg acagatcctt cttgccagct accggctcgc cttcgcttac 5281 cggcaggtcg tcgtcagcgg caccgccctc ttcgtccgga agctcagcgc cgaactggtc 5341 gatggccgct tccaagccag gcatcacgct caactccacc agcaggttca cggaagccgc 5401 cgaaagtgct tcctcaggga acaggcccga gtctttcagc gccttgaggg tgtcggcagt 5461 ggtcttgccg atatcggccc gctctttggc cgtcgcctgc caaagcggtg cccaggcgta 5521 atggatctct ttgggccgga cgccaagtgc tgagcggatc aggcactcat ccagaacgct 5581 cattgctggc ttgatctcca gcttctggcg tgaggcgacg ttgtcgtagt agttgcgggt 5641 gttctcttcg ccgttggcac caaggccggt ggaggactgg ccgaacatgc gggtgccagg 5701 tatatcgaac gcacccgaca cgccctgctc tgtcttggcg atcacctccg gcagattgcc 5761 gaagctggcc gacttggagc tatgcgtctc ggcaccgtct aggatcagcg tcccattgat 5821 gcccttggcg gtagcagcca gacgcagccg ctccagcagc aggcgctcgt aattcttgtc 5881 ctgcatgctc gacatcaggt tagggatatt gatgacgtcg atcttcgcct cgtagaccag 5941 gctcacaacg ttggctaccg tctcgtcgta gtgacgcacg gcaggcatag cggccagtag 6001 cacagagtcc ccccagccga agccgcaacc gacagccagt tcagggtcag ggtgcggcgt 6061 gcctatgaag atgaccaggc gcgacgggtg gatctccacc acgctgcccg gcagccggta 6121 agccttgggc ttgccgaagc gggggctttg cggatcctgt tcgatctcgg tcgcgctcaa 6181 ttgccggcga gtcatgacgg tgaggtactt cacgccaccc tgccgcacgc gctcagggtt 6241 cagctcggat gccgtgtctc gctccccggt gccgatgaat acagcagcac caccgaacag 6301 tcgggccttc aatagggcct caaggatctt gccctttacg ttcaggcggt cttcctcggc 6361 ctcgatgagc tcgatctgac ccttatcggc ttgccagctg cgccagttac ggcacgcgtc 6421 caccgcggga atggtcacgc ccttctgcgc agtccacgag ccccggaagg cgttcaggag 6481 ctgctggtcg tccatctccg ggatggcata gtgcgagtgg gatgccttgt ctcgcgcagt 6541 acccagtcct gcgaccaggt tctgcaggct gtctttcagg tagctgaatg cgctcattgg 6601 ttgctcacgt ttgcgagtgt gtagctgccc gcaatcggga agcgctggac aatgaagtag 6661 ccgagtgcgt cgaccgggtc ttcagtgccg tccttgttgg gttcgccgtt ttcgctatat 6721 gcctgctgct caagcacctg ggtggttact gggcagttgt cggtgttcac cagatagcgg 6781 cgcttatggt cgatattcag gaacatggcg ttcactgcca gtacccgatc gcgcacaatg 6841 gggttggatg ggttcaccat aaccatgaag ccggcggcgc ggagcaggct gtggtccgac 6901 tcgctgccgt tcacgctctt gcggttcttg ccgctggcgt cgggatagac cgtgatgctg 6961 tgcccgggga agcggcgctt cagctcgaca atcatcgccg gcgtatcgaa caggcttgtg 7021 gcctcttcca gcagcatggg cagtccgtca cggatgacat ggatcgttgc ggccatccgg 7081 ttgatgttga agtccatgcc gacgtgcagc tgctcgcctg ggcgaatagt cgcgtcggtg 7141 tggttctgct ttcggcagaa gctcgggtat acgctgcccg aggtcaggtt tacgaacagg 7201 ccatcgatgt acgcgtccac caggttcgcc gggtaggact cgcgcagcga cttgatgtaa 7261 tccttcggca ggttctttgc gttctgccgc gtcgaggcgt gcacgatgcc gtacagcggg 7321 cgctggctcg gattggatgc aagctccttg acgaacttgc ggtacaccca gttgaagccc 7381 tccggcgtcg tggtgacgtc gatggtgttc atgtcgcggc caggccacac agtggacatc 7441 cgcgcgatga tcttcttcca ggcgctgtcg gccttcttga tcggcatgca gtcgatctcg 7501 tcgacaagcg catgcgctat gttgaagccg acgatgcggt gcgggtgctc catgctcttg 7561 cagacgatgg tcgataggca ccgtcctcgg ctatcacgca ggtacacgcg cttcttgctc 7621 ggcacgatgt cagcgaacag gccgaaagcc tcagcgacca ccggcatggt gtcgtagaag 7681 atgtcagcga tctgcggata ggtcggagcg aagtagcctt ggggtatacc tgggaactca 7741 agcgcgttga cgcacatgcg cacgcagcct acaaacgtct tgccgctccg gtacccacca 7801 acgaacgcca tgaacttctt gtggcttttg atgaactcga actggggctt attcagcatc 7861 aggacagctt gcatcttcca ccccgatgat tacttgcttg ggctcgggca ggcccttatt 7921 cgggtcttcc agttcgcggc gcagcttctc gttggtgagt cgccgcgtct ccaggtcttc 7981 cttcgtccgc tccaggctct cgacacgggc agtgaggcgg tcgatcaggc tggagtagtc 8041 ccgaaccttg ctcgtagtcg tgatcgaggt gagcgaggtc tccttgccgt tgattaggat 8101 cggctcttcc ttctcagact ccacctcaag ggtgttgccg aattcgctct ccctggccag 8161 cgcacgcatc aagcgcactc tcgtcagccg caactcatcg tccacacgcc ccagctcaat 8221 gctggcaagc atgtcgttct cttcgtcagt caggaatcgg ctgtagatcg agccgggctt 8281 tgcagcgttc ttgttgcccc tctgttcctt ggggcctgaa ctcttgccgc cgtgtagctt 8341 gcagcgagag gaacccggta tcgcgtgacg cttgcatggt tccccgttgc cgcgcttgga 8401 tgcgccgcat agggccatag tcagcctcat tcatggggtt ggttttcgca gaacgattca 8461 ttcaatgcat tcgaacgtca ttcaatgatc attcgatgct taaaaccttg acctcacccc 8521 atacgggatg ggagtcgaca cgctcgccat tggatgactt gatcggctgg tcagctacaa 8581 cagcgatacc cgccatcgcg tcgcaccaga gcacatgcgt gacgggctgg ccgtcgatga 8641 tcacttcacg ccgccctcga ccatcgtccc agtggtggac gtgttcgcct gattggtcgc 8701 tcatggcgca acctcatact ggaacgcccg atcagcaggc atggccgtca cgaaccggca 8761 cctgtgagta tcgaacgcct cattgaacgc aatggtgcgg cggtactcgg aggtgacctt 8821 accggtgaac cgctcgcggt gaacgaccac accctgacgc aggtactcga cttcagccga 8881 tcccagtagc ttggtgacga ttactttcat gtgacctatc tcgcgccacg atttggcgca 8941 ttcgaaaacg tggcgcggat taatctacct ttcggcctgg cagcttgaag tcagtcaccc 9001 ggtcagcaat ggaccggatc ttctccaccc ccaggaagcc aacccagcca cctacgaagg 9061 tagccatgct ctgggggaga gagaagaact caagcccgct gatgatcgtc agggccaggc 9121 caccacagat agcgccctcc acgatcatct ggcgccgtgt accgccaccg taggtgattc 9181 gaaggacagc catcgcacag gacaacgcag ccgcatagag gattggcgaa tgctggctca 9241 accacgcaag cgctatcgcc caggtgtctg gtttgtctgg catgttggac atctcggttc 9301 ctccccgtca gggagttagg gatacgacag gccatgacct gcggaattga atcggctcac 9361 acagcactcc cagctcggag caatgggtgt ggtggagccg aaaactggaa aggcccgcgg 9421 agtgcggacc atttgtaagc gttagcgaag ctgcgaaatt tgtgcttcga gcgcggatat 9481 cctggcactc ataacggaat cggctgttat gcgtgcagaa cactcctgcg tgatgagcgc 9541 tttcgtgacg gtggcatcat cgatgaagcc ctggcggatg taggtcacac cgtccactac 9601 gatgaatggc ttgagctctt gggcatcacg cagatccgga aggttgccca gaacgacctg 9661 gggtgcaccg tttgtactga ttaccagctt tccgtctttg atgaccgtat tcgatcgctc 9721 tctcggcaag ccgttgatct cgaaggtacc ggtggcctgg tcaagcttcc aaccggaaac 9781 gcccggcacg tagttgctgg attgaataga gcaggacatt tgctgcgcct cttaacgaga 9841 aaagccccag cgattgctga ggccctgaat aggtgcgctc gtctttccga gctgtcggcc 9901 aaagaccatc ccagcgtcga cacccctttg catcgatctc gctgttcctg tctcgcgcca 9961 ccctgaaagc attgtgaggt cagagtgcgc gggctgccgg tgttgatccg tgcgtcgcac 10021 tatccgtcta tcgacgtcca ggccttcccg agagctgtcc tggctacagg tgaaactaca 10081 gattcttttt gtggatgcgc cagcccatgg cgacaccggg atgcaaatac tcgccggtgc 10141 gtggatggcg cgagaagtcg gtctcgccaa cttggcgcgc aaccgcctcc catgccgttc 10201 gggcccgctc cagcgtgttg attctggtct tcagcttcat gcgcacctac cggcagtaga 10261 attaaggcaa tcggcatagg ggacgg //