LOCUS MF417929 32618 bp DNA linear ENV 27-FEB-2018 DEFINITION Uncultured Caudovirales phage clone 2F_1, partial genome. ACCESSION MF417929 VERSION MF417929.1 KEYWORDS ENV. SOURCE uncultured Caudovirales phage ORGANISM uncultured Caudovirales phage Viruses; dsDNA viruses, no RNA stage; Caudovirales; environmental samples. REFERENCE 1 (bases 1 to 32618) AUTHORS van Zyl,L.J., Abrahams,Y., Stander,E.A., Kirby,B.M., Clavaud,C., Farcet,C., Breton,L. and Trindade,M.I. TITLE Novel phages from South African skin metaviromes JOURNAL Unpublished REFERENCE 2 (bases 1 to 32618) AUTHORS van Zyl,L.J., Abrahams,Y., Stander,E.A., Kirby,B.M., Clavaud,C., Farcet,C., Breton,L. and Trindade,M.I. TITLE Direct Submission JOURNAL Submitted (29-JUN-2017) Biotechnology, University of the Western Cape, IMBM, C2, Life Science Building, Robert Sobukwe Rd, Cape Town, Western Cape 7550, South Africa FEATURES Location/Qualifiers source 1..32618 /organism="uncultured Caudovirales phage" /mol_type="genomic DNA" /isolation_source="healthy skin from forearm of human male" /db_xref="taxon:2100421" /clone="2F_1" /environmental_sample gene 198..1349 /locus_tag="2F1_1" CDS 198..1349 /locus_tag="2F1_1" /note="Similar to YP_007236749.1 [Burkholderia phage BcepMigl] (BLASTp score : 173). Similar to PF00589.17 [Phage_integrase] (hmmscan score : 49.2)." /codon_start=1 /transl_table=11 /product="putative tyrosine recombinase" /protein_id="ASN71602.1" /translation="MGTITKRITSTGEVRYRAQVRIKRKGLPDFTESKTFSKKSLASE WVKKRESEFERDPEALINQNKTSGLTLAEALEKYLLEVAGFGRSKRMGIKFLTGWPIG KILLRDLKRQDFTDHTLLRKNGNPDLAVGPISASTALQDLQYIKTVLNYAELSWGEPV NIYELEQAMRGLRHARIITKSEKRDQLYTSEQLQQLTNYFYRRWMLGKTAIPMHLIMW LAIYSCRREAEITALYLKEFDRYHMEWKVFDLKNPSGSKGNNKSFIVTDLCLTVIEEL LKPEVRGRMLKLGRNNPDVLVPVDAKSYAARWREGIKMSGLNGLRFHDLRHEGITRLA EDGLTIPQIQQISLHESWESLRRYVNLKNRRERLNFTEAIDVAKDIYES" gene complement(1409..1699) /locus_tag="2F1_2" CDS complement(1409..1699) /locus_tag="2F1_2" /note="Similar to PF11112.3 [PyocinActivator] (hmmscan score : 54.2)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN71603.1" /translation="MKNALDQFDSSFLLAMKYKLPVVTLETVVADFMPYLKIETAKKR ASKQDLPFPAFKAEQNSKAGWLVKITDVAIWLDSEHAISAKDWNNMKSSQRD" gene complement(1696..1914) /locus_tag="2F1_3" CDS complement(1696..1914) /locus_tag="2F1_3" /note="Predicted by MGA" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN71604.1" /translation="MFNVTPDHFLAKEAYEKAKSLKTSYVHILGSSYGIKGKEQYYLS GVVGNNSEIGFNRLDWLTEYEELNGIQI" gene complement(1917..2192) /locus_tag="2F1_4" CDS complement(1917..2192) /locus_tag="2F1_4" /note="Predicted by MGA" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN71605.1" /translation="MNKAPLNLNSIPDLNLRQIEVLIEELENTYATGHADHPMLKVVN VSIELTWKSGSVTYTLPQLKAMKAELILKTQCPQNSEELPFIDPEQR" gene complement(2185..2601) /locus_tag="2F1_5" CDS complement(2185..2601) /locus_tag="2F1_5" /note="Predicted by MGA" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN71606.1" /translation="MQSDSNLDTLLLKQFKTVMANDLTSLDWDEELEMFTDPNTQISW LTFLNTLDFYWSFKGKTVQDFPANYILIRKELSDIEAQKLAEEKVRRISKKIDMKYGF KAEHIQEKKAQLIRVETNFIKKTHRDVVEQWSNIHE" gene complement(2670..5399) /locus_tag="2F1_6" CDS complement(2670..5399) /locus_tag="2F1_6" /note="Similar to YP_009067124.1 [Ralstonia phage RSY1] (BLASTp score : 694). Similar to PF13155.1 [Toprim_2] (hmmscan score : 35.6)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN71607.1" /translation="MDLQRRIDDRINQLFGFKRKGEWYREGRCPKCNEKELYTHAETP RIIKCGRLNKCGYEEHVKDICEELFKDWSEYHPKTPENPHAAADAYLKEARGFDLKPL KGLYSQELYQSPKNRSLISATVRFKLAEGIYWERLIDRPERFGRQKANFIGKWAGLAW TIHKLDELCNAGSIWITEGIFDSISFSQSEVISFSIMNSGNYPTLLLEQIKKRCHELN RDKPRLVWALDNDKAGKSALSKHHKRATDEGWISTAALPPAPVNGKSFDWNDLYQREQ LTKEHLETYIHYGKLQIVETPEEAGLLIYNFYGSNLSKFFFNHRFRTYWWELDYEKFN KAVQYVEESKQDQVLTDEEIRIQALKTCSSAKEICNAQLEPLYFQRNEITDESWYYFH LHSPWGEVKTTFTAEQMSSRSKFKPRVMSVLSGAMWTGSDQQLETFIKRKTEKLREVK TIDFIGYSKEYKTYIFDEYAVNKTQVIPKNEHDFFKAGNIEVKTLASSPVIRLNPKKE FKPDWWKDFYSLQGEKGLILLAWWTGSYFAEQIRAINSSYPFFEFVGQAGSGKSTLIE FLWKLSGREAYEGFDPNKSTSVAIYRNFAQTSNMPIVLIEGDRNDQQGTQKAKFSWDE LKDAYNGRAIRSKGLKTAGNETYEPPFRAAIMISQNTPIQATEAILSRTLHISVDTKN HSLDKKHIATRLTQMSLEDACTYMTYCLKNEEKILKTYDEKHREIEIEFHQKGITHTR IALCHAQVSAMIDAIAEHIFKDVMDLSDICDAKRYLEEMARRRVDEIAADHPMVQQFW DAFEYLNSVRTSSFHLNHYKTGDAHIAINLNEIYKVAARNFQALPEINEMRNLLRTSK RYKFIEANKPVRTSKHPADEVNNLSPGYGDKPPKEDRILKCWIFTNPNFGATK" gene complement(5802..6041) /locus_tag="2F1_7" CDS complement(5802..6041) /locus_tag="2F1_7" /note="Predicted by MGA" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN71608.1" /translation="MPNSAIQQQLQIKNSFFCCPNCEQPLTVLGTIVVSKEEPRAYVG ECRELTCMTLVHFHIQLSNVIAPSRLINISRNIER" gene complement(6063..6257) /locus_tag="2F1_8" CDS complement(6063..6257) /locus_tag="2F1_8" /note="Predicted by MGA" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN71609.1" /translation="MKSSENINKSDKQIAFRVTEKEYKDIMIQAALDSSLSPNQYAKQ VVLNKINTDSLDQIQKLKES" gene 6383..6700 /locus_tag="2F1_9" CDS 6383..6700 /locus_tag="2F1_9" /note="Predicted by MGA" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN71610.1" /translation="MNSFYDEGRGNRLKDERKKFNGLTQKAAAEIAEVKEQAWVRYEK HGAPFDLKFALLLQEYGFDMMYVIFGVRKTLNPEQTQLLGIFEKLDDNQKQKALQMLE LIK" gene complement(6689..6907) /locus_tag="2F1_10" CDS complement(6689..6907) /locus_tag="2F1_10" /note="Predicted by MGA" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN71611.1" /translation="MKDIQMFKLNENELYELRQLKNKVSFILALNLKGGDTSPDITST ELATIFGEINQQLETVISNIEEANESFN" gene complement(6917..7225) /locus_tag="2F1_11" CDS complement(6917..7225) /locus_tag="2F1_11" /note="Predicted by MGA" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN71612.1" /translation="MKIGSIIIKVQNPANNKRQFFVSSKKLYNLLDPESTYKTFVETN IVWSRLRENIDYHYNERFETYNLSISSVQAILILENTERSWQFFNELSDLINSGFKTV " gene complement(7242..7916) /locus_tag="2F1_12" CDS complement(7242..7916) /locus_tag="2F1_12" /note="Similar to YP_005098457.1 [Erwinia phage PEp14] (BLASTp score : 76.3)." /codon_start=1 /transl_table=11 /product="putative O family protein" /protein_id="ASN71613.1" /translation="MKKVLLSTNEYPIIASPQLAKELGMPAATFLQKLYFLLNETKKY KNKKNLTQHNNRRWWFHTYEEWVSTLGLFSVSSIKRAVSKLKQLGLIEINKLDPNKSM RVNYYTINYKKLKELFGIGVEVKASAPAATKGNNQKIVGTDTPACPEATAEDLATLQS QHRALYRQLRGLKVDITHDDPLIFKFADRPREVLAYAASASSRLDINRWQWHTVEQIL PQHILQ" gene complement(7913..8095) /locus_tag="2F1_13" CDS complement(7913..8095) /locus_tag="2F1_13" /note="Predicted by MGA" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN71614.1" /translation="MSKIYIHTELGIEKRCTCCNEYFPFDDEFFYKNGFKHGIQQWTA RCKACYVETYRGGFGL" gene 8846..9538 /locus_tag="2F1_14" CDS 8846..9538 /locus_tag="2F1_14" /note="Predicted by MGA" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN71615.1" /translation="MIIGLINPKWIQLKNRKELTRKRIILLGLGATILLTIIGVSTNP SEEKIPGENITSSSPSELKNNDQNPSNEVKQEANLGMTPEQFRSDFNKRLKTLDVHMI RPLAEFDIEKGEVRDIFQVPFSNQINMTGTVNKDGMLREITFIIVPNEDSAQVMLDTL TLINTASNTVNSSADKNITGKVVVDLVQKALEGADQDNNSHSQVVGNIKYYALASKYT GLWFGLSPVDDK" gene complement(9604..9861) /locus_tag="2F1_15" CDS complement(9604..9861) /locus_tag="2F1_15" /note="Predicted by MGA" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN71616.1" /translation="MQAIFKTNLSYFFLLSCKERAIILLSSNQTKLFKMKDLEKTQMY IEMLNERIEELKKADDQNPYAKICVTHLNDELSKLIKFLTP" gene 9912..10091 /locus_tag="2F1_16" CDS 9912..10091 /locus_tag="2F1_16" /note="Similar to PF07927.7 [YcfA] (hmmscan score : 60.6)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN71617.1" /translation="MKYSEFERWLRQQGVEVVRSGKGSHRIVSYKGKKTTLPFHKGRE IGESLRKEIIKQLGL" gene 10208..10624 /locus_tag="2F1_17" CDS 10208..10624 /locus_tag="2F1_17" /note="Similar to NP_892103.1 [Yersinia phage PY54] (BLASTp score : 123). Similar to PF03681.10 [UPF0150] (hmmscan score : 35.0)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN71618.1" /translation="MQYPVTLTHDTDGSFLVRCRDIPEALAVGYSEEEALAEAINGFE SAFMIYISERRTIPLPSKKLKDEYLVRLPVRVSFKVALYNEMLAQDLKKADLARRLNW DQKQVDRLLSLKHSTKLESLEEAFHVMGREVDVVVA" gene complement(10767..11753) /locus_tag="2F1_18" CDS complement(10767..11753) /locus_tag="2F1_18" /note="Similar to YP_008239582.1 [Salmonella phage FSL SP-004] (BLASTp score : 232). Similar to PF05954.6 [Phage_GPD] (hmmscan score : 138.7)." /codon_start=1 /transl_table=11 /product="putative tail protein" /protein_id="ASN71619.1" /translation="MIKTPVCIITANARPLNQLIAERILSVTVTDNRANEADELSIVL DDHDGALELPKRGVKLNCQMGFKGEVIHDKGDFIVDETEWSGTPDLITVKASSANFKS NIKEAKSKSYHRKDFGQIAGEIAKKHDLSLVMTDDLKTIDLVHVDQTNESDLNLILRL AKQNGAEMAVKKDRLLIFKAGTAKTASGKDLAAITLTRRDGDQFRYSEQDRESDHTGV TASYQDTSKGKREKALTGEKGKVKHLKGTFANKAAAERASRAKMAEIKRQMAKFSIVS AYGIPAISTESPVKLDGFKKEIDEKKWIVEKATHTYNKNAGLSTQLDLEAGL" gene complement(11750..12172) /locus_tag="2F1_19" CDS complement(11750..12172) /locus_tag="2F1_19" /note="putative gp11; Similar to YP_004306855.1 [Burkholderia phage KS14] (BLASTp score : 126). Similar to PF06995.6 [Phage_P2_GpU] (hmmscan score : 113.6)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN71620.1" /translation="MSLGQFIFQTSTLAFLEIQRQRNWNYSDNAVAFGRPKKQFTGAG SDTVTLPGLIYEEHGFGTRFALDVLANMADTGQGFVLMDGSGYLYGVYVIDSIDETKS VLIDNGVPRKVDYTLKLSRTDDERIETQTAPQQQENTA" gene complement(12189..14789) /locus_tag="2F1_20" CDS complement(12189..14789) /locus_tag="2F1_20" /note="putative gp12; Similar to YP_004306856.1 [Burkholderia phage KS14] (BLASTp score : 602). Similar to PF10145.4 [PhageMin_Tail] (hmmscan score : 99.8)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN71621.1" /translation="MGNTKQMSQISLKAMLELVDKATAPLRNIMGSSEKASDALRAQR EELKKLSKSQSDITSFRRLSTALKGTRSDLESAQQTVARLAQEHASVQRPTRAMIKEF EKAKLAVKSLKEAEQDQLRQLQMLRNGLNQAGISTKTLGRDERELKARIDASSQSLQR KKQQLDKQIASQKRINDLINQQKNTQEIISKISDTGMRAGAGAAVGAGALSVPIKAFA EAEDAATTLRVSMMQSNGQVAKDFREINELANQLGTKLPGTTAEFQLMMAKLVQQGIS YKAILGGVGQAAGYLAVQLKMPFEEAAEFAAKMQDATKTSEKDMLSLMDTIQRSYYLG VDSTNMLQGFSKLSAGMKTIKAEGLKGAQAMAPLLVMADQAAMAGESAGNAYSKIFAA MMDTKGIKKALKGSKLTMNFTNGKGEFGGLDNMFKQLSKLRKLSTEARLPILSDMFGN DAETIQALNLLIDKGKAGYNETLAKMNAQADLQRRVNEQLGTLKNLWDAAAGTFTSAM TNFGAAIAPELKQAVTGLTDVSEKIGDWSKANPQLSNTIMKVGAAIVILLALFSAISL VLVTLLGPMALLRVTLGVLGAKGFGLINIIKLMCGAFLWLGKSLFMVGRLMMTNPLFL AIGLLALAAYLIYRNWAPIKLFFTSLWNGIGSSAATLCQNLKAFFSSGIANISATILN WSLLGLFYRVFASVMSYFGIQLPSTFTGFGQMIMQGLANGIGNSVGAVVAKARAAAGA VIANVKGAFGIHSPSRVFAELGAYNMQGLANGISNNSHLANTAVTTASKDMLGYFDTG SIKFDSRPSIAAGNKSSTTAAQPIVQHFHIHPAPNMDEKALAQLVAIEVAKAQRLPSV GNIRGYSDID" gene complement(14902..15228) /locus_tag="2F1_21" CDS complement(14902..15228) /locus_tag="2F1_21" /note="putative bacteriophage gpE; Similar to YP_001165278.1 [Ralstonia phage RSA1] (BLASTp score : 85.5). Similar to PF10109.4 [FluMu_gp41] (hmmscan score : 41.5)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN71622.1" /translation="MNTEVQEQNQQAIQDPDIKPVKFDVGFKRGEQFIKEITIRKPKT RALRGLTLVNLLQLDVETLAKLAPRITSPTMSENDVYELDPVDLTKLSKEVVSFFVRA EDEDFQ" gene complement(15274..15780) /locus_tag="2F1_22" CDS complement(15274..15780) /locus_tag="2F1_22" /note="Similar to NP_046779.1 [Enterobacteria phage P2] (BLASTp score : 125). Similar to PF04985.9 [Phage_tube] (hmmscan score : 167.6)." /codon_start=1 /transl_table=11 /product="putative major tail tube protein" /protein_id="ASN71623.1" /translation="MLPRTLKNFNVFVNTHSWAGVAESVTIPKIAKKTEDFRGAGMIG DIALTMGYEKLEGEVTYAGFDVKQYRQLGVCGVSDLPVRFVGVYERQDNCTTQAVEIY TRGQAIELDPGDSKNGEKTEIKMSYNYSYYRMEVDGVVEIELDFINGTERFGSGDLAQ ELKKLLGL" gene complement(15784..16413) /locus_tag="2F1_23" CDS complement(15784..16413) /locus_tag="2F1_23" /note="putative gp16; Similar to YP_004306860.1 [Burkholderia phage KS14] (BLASTp score : 226). Similar to PF04984.9 [Phage_sheath_1] (hmmscan score : 70.1)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN71624.1" /translation="MAELSLYGYGIHTAVVVAAALRAETDSKVGFHKSLSNIPVTGPT GISQPITWDLEDPDTDAGYLNSQDITTMIQHQGFRFWGNRNCSDDIRFSFEVATRTAQ FLLDTIIQGCFPFVDQPLTPFLAKDIIDSINAKLREHVDAKHLIGASVWYDAAENSVE QLSQGKMWIDYDYTPVPTLENLGLNQRITDRYLVDFAQLINGQVTTEGI" gene complement(16428..17750) /locus_tag="2F1_24" CDS complement(16428..17750) /locus_tag="2F1_24" /note="Predicted by MGA" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN71625.1" /translation="MTMVSCKKLDKNDSELMPIILSAKLLVNGVQHKFTDLTSGALSH LLSPDADETSATDVPAGYINQGSTKLKNISNQTIRLKVDFTENNDNHDVIILPPNNSK NPTYYQGSDKRILEVCLAPGEPITIPSGSFSPLNVTHDGAYLYGFLTNNSGFGVYPLY GSQVDTSQNIFSKVLDHDSDVRFSADGSVLISNKFSLYRTADGFYDLEIAFNPPLMDT AFLDVNPDASYILAGTMGKNVEIYQRQTSTLYQKIGEIAEEDYFTGLAFSQEGDKFSY VTSGNADLKVYSFSQGVATQIGNRLGMADSGEWGLRTLSPPVWLHNNIIVRSYSQDSS QGVHFRGFVDVIRVDADSLNLVTRNEEDLGDTYYRFKTPRNGRVLATTDKNELKLLSV NTSGQIEFTHDYSPLINGYYVLMPSGSEIVTNSSTLGQVDIIHVDQPS" gene complement(17792..18412) /locus_tag="2F1_25" CDS complement(17792..18412) /locus_tag="2F1_25" /note="Similar to YP_008766939.1 [Enterobacteria phage fiAA91-ss] (BLASTp score : 141)." /codon_start=1 /transl_table=11 /product="putative major tail sheath" /protein_id="ASN71626.1" /translation="MSEFHHGISGRESASGNIPFRDANTSTITLVAIADDADEASFPL DTPVLVTSINRALPKAGLTGNLRKHLEIISAIGSPTLVVIRIADPFKNGQFNQSLVIG TTDSSGKRTGLQAALTVKSVLGITPKIICVPDTETLDVTNAIGAICKKLRAYSYITPR SNTGEILNSAQEVAAFRQKIAFREVEIIWPEFTSGNVFLGKSQAPQ" gene complement(18500..20176) /locus_tag="2F1_26" CDS complement(18500..20176) /locus_tag="2F1_26" /note="Similar to YP_009100034.1 [Escherichia phage vB_EcoM-ep3] (BLASTp score : 79.0). Similar to PF12571.3 [DUF3751] (hmmscan score : 64.8)." /codon_start=1 /transl_table=11 /product="putative tail protein" /protein_id="ASN71627.1" /translation="MTAKYYIALTDYGAELIAKAHQESSIALAYLVIGDANGIPYDPL DKKNLTSLINQCAQVPIQTVEIIDTVTRVTATVNSEIGGFNIHEIGLTDSSGKLVYIG NYHGAYKPVITDGGSGELEIVIDIKTNTSTNVLIQIDPNIVTANKKWVEKEVAKLELK FNSLVEAIYHVESWHGSNKKDYDPSLFLEPLFGYRTSWMIWPYIPAGVESVNDPLGTI SELQIGNSSRQASSTRIWQRLPDGSSPPSYTLTVNKSIANEGDEVIFTLITTGLNAGT PVDWTITGLGIKEDDFFPRILSGQFVVDTEGKAVHSIQITEDNKTEGDENLVFSLKYI KNKQVSVLIKDTSKYPEGSSVYYEGTHQIEILPNQTVKFHLHGPCGGGGGSIYSGTGT EPDGQNAGDVSCTIGTSTLIAGGGKAGTGGVWGNGSSYTEGKPGTGGITSVNDPEAIF DELEITNGIDAILYSRWETQKGASALPAIIGQMSGGGNGAVGIGDERHSFGGAAGSGA RIRAMFTNTTEEKILASIDIGKLGEGWKSWGNKATDGGIGFAIVEIFNPD" gene complement(20187..20711) /locus_tag="2F1_27" CDS complement(20187..20711) /locus_tag="2F1_27" /note="Similar to YP_009008375.1 [Stenotrophomonas phage Smp131] (BLASTp score : 88.2). Similar to PF09684.5 [Tail_P2_I] (hmmscan score : 105.9)." /codon_start=1 /transl_table=11 /product="putative baseplate assembly protein" /protein_id="ASN71628.1" /translation="MYRLLPPNATKLEKNVEKLGEKISNLAVPFIDLHRIDRCPVDHL PWLAWEHRVEYWRPDWTEIEKRNAISESKSFNAGRGTRSSIASLLSTVVENFQIKAWH EFLPPQQPFTFVVIIHPKHLISIEQLLQVHTAIDATKSARDNYSISAKVKTECNFYIT GSVTSGTRIHLTSI" gene complement(20704..21603) /locus_tag="2F1_28" CDS complement(20704..21603) /locus_tag="2F1_28" /note="Similar to YP_009100031.1 [Escherichia phage vB_EcoM-ep3] (BLASTp score : 229). Similar to PF04865.9 [Baseplate_J] (hmmscan score : 141.4)." /codon_start=1 /transl_table=11 /product="putative tail protein" /protein_id="ASN71629.1" /translation="MSTLNRVDLSSLPFPNVLEQVDFETELQACKDELVSRDPELVEA LSFESEPLVKLLETFAYRFLLKTGQTNAKAKALMLAYATGADLDHLASNRGVYRLTII PAQPNANPPVEAVMESDEALRRRTHLQPESMSAGSTGAYQFWGLSSHGHVKDIAVETP KEGHVNIWVQSHLDEIASQELLNIVDQTLDPDTRRPATDWVHVKAAEPETWQLNATLV LFPGPDSAVVKAAAEADALLYTQQTSSLGYDVTRSGLFRALHQGGVQNVVLDSPAADI VLTKNKYAKCTAIRINIVEFRDV" gene complement(21600..21965) /locus_tag="2F1_29" CDS complement(21600..21965) /locus_tag="2F1_29" /note="Similar to YP_001165269.1 [Ralstonia phage RSA1] (BLASTp score : 90.9). Similar to PF04965.9 [GPW_gp25] (hmmscan score : 61.6)." /codon_start=1 /transl_table=11 /product="putative baseplate wedge subunit" /protein_id="ASN71630.1" /translation="MKGMSRYTGKKLTDSGQLPAHLEQSLHDLLSTLIGTRICRRDYG SLVPHLIDQPCNDITKLKIMNGSATAIIRFEPRLKIKQVQVSSTEVESAWNVTLIGSY KQYGQEQPFKQSYTFGATA" gene complement(21962..22504) /locus_tag="2F1_30" CDS complement(21962..22504) /locus_tag="2F1_30" /note="Similar to NP_490615.1 [Pseudomonas phage phiCTX] (BLASTp score : 76.6). Similar to PF04717.7 [Phage_base_V] (hmmscan score : 54.4)." /codon_start=1 /transl_table=11 /product="putative baseplate" /protein_id="ASN71631.1" /translation="MSMTNQISRQFQNLASIGTVIAVDATAGKMRLQIDENETDWLPI PTMAAGIVKIWRCPSLGEQFSVSAQGGELTNAVPQVSLFSEENPPPSTDPDEVIIQIG EHFLSFKVSSGEAVFKFDKCIFDVAETTFKGKVHAENIISSDKDVKAKDISLIEHLHP GVMKGTGQTEKPAPTGGMSK" gene complement(22582..23427) /locus_tag="2F1_31" CDS complement(22582..23427) /locus_tag="2F1_31" /note="Similar to YP_001039848.1 [Vibrio phage VP882] (BLASTp score : 206). Similar to PF02086.10 [MethyltransfD12] (hmmscan score : 112.1)." /codon_start=1 /transl_table=11 /product="putative DNA adenine methylase" /protein_id="ASN71632.1" /translation="MTTMSPKTNPKEVTKPTYNASGRSFSGWLGGKSQLARTIIDMMP EHKHYCEVFGGAGWVLFKKSPSTLETINDVNGDLINLYRVFKYHPDALEKEFETQLIS RDEFDRLKAQNVASLTDVQRAARFYYLLRTCFGAKIEGPTYYSHAERLPTLKLGSDLK DILSAINQRLQKVNIENRNFDVLIEKMDRPETLFYLDPPYYNCENYYGKDIFGRDDFI KLRDLLRNIKGKFILSLNDVPEVRELFAGFYFHTRQIRWSLNVKSQSEQNGKEIIITN FKIPD" gene complement(23574..24014) /locus_tag="2F1_32" CDS complement(23574..24014) /locus_tag="2F1_32" /note="putative gp39, phage virion morphogenesis protein; Similar to YP_001111070.1 [Burkholderia phage phiE202] (BLASTp score : 81.3). Similar to PF05069.8 [Phage_tail_S] (hmmscan score : 100.1)." /codon_start=1 /transl_table=11 /product="virion morphogenesis protein" /protein_id="ASN71633.1" /translation="MIDAYSGLAHWLDQIAVRLEPDQRRELMRRLAQGLRVRHRDRIK QQRDPGGNRFIPRKREQIGSKKRQGALFQNIGKQLKTEYSADHAAVGFGGRTATIASI HQEGKTIKPSRNAKATSYPIRELVGFSNDDEKWIRSEIQKFLST" gene complement(24011..24406) /locus_tag="2F1_33" CDS complement(24011..24406) /locus_tag="2F1_33" /note="Similar to PF06891.6 [P2_Phage_GpR] (hmmscan score : 37.8)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN71634.1" /translation="MKALIPLKSYLSEKIPTLSADKCHLLIVNGTQAKGYLEYTARLL FLDFRGDPIEVIMLIRNWLKAHNLNLDSTGQDVLLSFSSEVIDTDTFDLEIDFPQRDK VVMNENGPHICPQMVWSDKHGKFIPAGSE" gene complement(24409..24930) /locus_tag="2F1_34" CDS complement(24409..24930) /locus_tag="2F1_34" /note="Similar to YP_006990464.1 [Edwardsiella phage KF-1] (BLASTp score : 185). Similar to PF05838.7 [Glyco_hydro_108] (hmmscan score : 87.9)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN71635.1" /translation="MELTFEQAFERLIGHEGNFTDDPKDRGNWTTGIIGKGQCKGTKF GISAMTYPDLDIKNLTLERAKQIYRRDWWDRISADEIHPAIVFQVWDFAINSGMGTAK RKLQKAVNVAEDGIIGKKTLKAIQDSDLNDVLLKFNSFRLLHLSSLSTWPRYGRGWTI RVAHQLNYAALDN" gene complement(24941..25312) /locus_tag="2F1_35" CDS complement(24941..25312) /locus_tag="2F1_35" /note="Predicted by MGA" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN71636.1" /translation="MNDPISIKGLPWLLKIIAAILGAVLALILSGDIDTQGRIKITLG VIIKFTISVAISLYGGSACIEYYQLTKYSITSQGFVMLIFAVFGMLIIGIWYQSLQLM RGKALYEIVAEVKAAFTAMFK" gene complement(25326..25529) /locus_tag="2F1_36" CDS complement(25326..25529) /locus_tag="2F1_36" /note="Similar to PF05489.7 [Phage_tail_X] (hmmscan score : 40.7)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN71637.1" /translation="MNTVYALQGDTLDAIAYRYFKDNPVQMLAALIELNPDLHEIFLN EHQAVLLPDATQIQSPPTIKLWD" gene complement(25526..25924) /locus_tag="2F1_37" CDS complement(25526..25924) /locus_tag="2F1_37" /note="Similar to PF05926.6 [Phage_GPL] (hmmscan score : 54.7)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN71638.1" /translation="MLLNASVPDHIVKNPEPDRPDVSIKDLMGMVRLDLSKGTELLAE KIVLAMDDINDQTLLLKIETDEQIRKYKRAVCYEAAALICEENLDFDTTTGGQSRGEN QQIKAQSLRRIVNHSIASLTNKPRNRVKLV" gene complement(25930..26757) /locus_tag="2F1_38" CDS complement(25930..26757) /locus_tag="2F1_38" /note="putative gp38; Similar to YP_004306450.1 [Burkholderia phage KL3] (BLASTp score : 106). Similar to PF05944.7 [Phage_term_smal] (hmmscan score : 91.4)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN71639.1" /translation="MSNSMRQNRERKLAEKAVRLAQSPDPRLRKKKMSMGFDPGSPEG DYSATVPVENSPTGNIELRMFSQLQQLADIRSIQEKIAKKAEWLPEYAGYIEGCLVTS PAPQNNVLVRLMIWAADVGDYEQAVRIAEFALLNEMVMPEGHTRSIAEFITEQCSQDF IKDHELAVKNASVIEKIIEIGTGESMVDEVRAKGFRALGDALRDAQPVEALNAYKNAL RFNTNAGCVKQVTQLEKKLNLQPTESSPDATVGSQADASSVSTENESVPASTDTTPE" gene complement(26757..27785) /locus_tag="2F1_39" CDS complement(26757..27785) /locus_tag="2F1_39" /note="putative gp33; Similar to YP_004306877.1 [Burkholderia phage KS14] (BLASTp score : 288). Similar to PF05125.7 [Phage_cap_P2] (hmmscan score : 331.6)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN71640.1" /translation="MSVVLHPIARTKLSSYITDIARANNVEDARHTFAVQPAPEQKII AAYQESADFLKQINIFPVDNARAEKIGLQIGTTVAGTTDTRKTPRSPVAVGSLDLLDE YECTQTNYDVAYYWSLLNAWKHHPDFKAKLQAMVIKAIALDKLCIGFNGLYRAPTSDR VANPLLQDVKKGWLQKIRENAPEQHYEGVDDGTGKLVTKISANSEFKTIDGLVEFAVE EYIAEQHRDSGLVVICGRGILSDKYLPLLNTVQDPTEQLAARTIYANKQLGTLPALHV PKFPAKTILITKPDNLSIYLQSGTLNRSIVEQPEWDRAVDFQSVNEDFVVEDYAKCVL IENIEVTA" gene complement(27804..28703) /locus_tag="2F1_40" CDS complement(27804..28703) /locus_tag="2F1_40" /note="putative gp41; Similar to YP_004306408.1 [Burkholderia phage KS5] (BLASTp score : 136). Similar to PF05929.6 [Phage_GPO] (hmmscan score : 164.2)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN71641.1" /translation="MEICMELAGAGRVEKRFRVAREGQTVDGRELTREEIQQMAATYS IEKYGARINLEHFSGWSPEPPFNAYGDIIKVEAVEEDGKYSLYNTISALPNFIELNKK GQKIYPSIEFYRNFAGTGLAYQVGLGLTDTPASLNTQPIKFSGNSFSLRTHPNTEIFM SAPAAPAEQNNSANTEQKGFFEQLKTALSGQPKTPPQPDDFQEVVTKGVVTALNGIKD LTEKFNTLQASITPAPATTPAPAEVITQSTVPANSSEQFTQVMAPVMEALTGLQNQFK QIQSTVVNNPPAQTGGDADQVTY" gene 28905..30611 /locus_tag="2F1_41" CDS 28905..30611 /locus_tag="2F1_41" /note="Similar to NP_490600.1 [Pseudomonas phage phiCTX] (BLASTp score : 503). Similar to PF03237.10 [Terminase_6] (hmmscan score : 218.7)." /codon_start=1 /transl_table=11 /product="putative terminase ATPase subunit" /protein_id="ASN71642.1" /translation="MSVSEIAKQIDEKRATVESWKQRDGWEKADLFDDVTLGLKIRYM ALTFMESKGNNHYKEMDFIGVQFERWARIERFRNGGTQADLNPKIENRNDKPRKKKLK NEITEEDLKLLEQAFLDLLFIYQREWISAIDESDIFILVKSRQIGATHIIALWSLIDL LKTGKNKIFMSASKAQAYQFIEYIKAFALEICGIDLTGDPIVINGPVKQATVYYLGTN ALTAQGRHGDVIMDEFFWIRKFLQFKKVASAMASQKMYKEIYMSTPSSILHEAYKFWT GTDSGRKLPVEIDVSKSALKMPVKCADGKTRQIVTLADAEAKGCDLFDREKLLNQYGD EEYGNLYDCEFIDDAGSYFPLKIITPNMVDSWMVWKDFKPNETPHYIGEVWLGYDPSF TGDNAALAVIAPPRTPLEPYRILEVKQFKGLRAEEQALYIKKVCNRYNVSFIGIDNTG NGIAVAEHVAKFFPSLTRLNYNPDLKIRMGLRAKELFQKRRLHFDAGLTTVAKAFLSI KKALTSGGGNKTLITTRSAENGHGDVAWAIMNGLEKAPIVDTTDVTQQGTHSSRIRVY KS" gene 30608..31672 /locus_tag="2F1_42" CDS 30608..31672 /locus_tag="2F1_42" /note="Similar to YP_001718748.1 [Salmonella phage Fels-2] (BLASTp score : 348). Similar to PF04860.7 [Phage_portal] (hmmscan score : 165.7)." /codon_start=1 /transl_table=11 /product="putative portal vertex protein" /protein_id="ASN71643.1" /translation="MNLKNILSGSLQRFTSSPAKAIAESAQKTSIVCRNFGEPEPVLD GHTLFEYGYCPKWLDWYDLPYDMLATAKLFRATSHHTSSLIVKRNILTSDFIPHPWLS RHDFNSLALNLLTFANCYVHIKRNAFGGIVGLGSRPAINMRRGLDLASFFQLGYDQTD NYRFEHRDIIHLYESDISQEVYGVPNYLSSINAILLNESATLFRRRYYKNGAHAGFIL HMTDALQRQEDIDALEDALENSKGAGNFKNLLLYTPGGKEKGVNVIPLAEVAAKDEFY NIKIASRDDQLAGHRIPPQLLGVVPQNAGGFGDVEKAAKVFYFNEIIYYQNLLKQINE RLGVEVINFKKYELLSTDKE" ORIGIN 1 atgttgtcta gtccatcata ggtgcaacgg atatacgagc atttttataa ctatcaatag 61 tttgctgatt atcaaaaata ttttgtgttt ctatcatttt catttgctcg catttaaccg 121 cattttatcg catataatcg gaacatggtg caccatattt gcaccacaga atttaaaaat 181 tatcatggtg caaatttatg ggaacaatta ctaaacggat aacatcaacc ggtgaagtac 241 gttatcgtgc ccaagttagg attaagcgaa aaggattgcc ggattttaca gaatctaaaa 301 catttagtaa aaagtcttta gccagtgaat gggttaagaa aagagaatct gaatttgaac 361 gagatccaga ggcattgatt aaccagaata aaacctctgg tttaactttg gccgaagcat 421 tagaaaaata tttattagag gttgctggtt ttggtcgctc aaagcgaatg ggtatcaaat 481 ttctaaccgg ctggccaatc ggtaaaattc ttttaagaga tttgaagcga caagatttta 541 ctgatcatac attgctaaga aaaaatggaa atccagactt agctgttggc ccgatttcag 601 caagtactgc gctccaggat ctacaatata taaagactgt tttgaattat gctgagctta 661 gttggggcga gccagttaat atttatgaac ttgaacaggc tatgcgtgga cttagacatg 721 caaggattat cactaagtct gaaaaaagag atcaacttta tacatctgag cagctgcagc 781 agctcactaa ttacttttat cgtcgctgga tgctgggcaa gactgctatt ccgatgcatt 841 taattatgtg gctggctatt tacagctgca gacgtgaagc agaaattact gcactctatt 901 taaaagagtt tgaccgctat cacatggaat ggaaagtatt tgatttaaag aatccaagtg 961 gcagtaaagg taataataaa agcttcatcg taacggatct atgtctcact gtaattgaag 1021 agctactaaa acctgaagtc cgtggacgta tgttaaaact tggtagaaat aatcctgatg 1081 ttcttgtgcc agtagatgcg aaatcttatg cagcacgttg gagagaaggg ataaaaatgt 1141 ctggtttaaa tggattacgt tttcatgatt taaggcatga aggtattaca agactcgctg 1201 aagatgggct tactattcct cagatccagc agattagctt acatgaatcc tgggaaagtt 1261 tgagacgcta tgtaaattta aagaatcgtc gtgaacgcct taattttact gaagccatag 1321 acgtggccaa ggatatttat gagagttaaa tcaagaataa taaaatagta aaaatgccct 1381 tagaagaggg cattattttg gaccaaattt aatcgcgctg ggaacttttc atattattcc 1441 agtcctttgc cgatatagca tgttcactat caagccagat tgctacatcg gtaattttaa 1501 ctaaccatcc agctttacta ttttgttcag ctttaaaggc tggaaatggt agatcttgct 1561 tactggcacg tttctttgct gtctcaattt ttagataagg catgaaatct gcaacgacag 1621 tttccagtgt gactacaggt aacttatatt tcattgccaa taagaaacta gaatcaaatt 1681 gatcaagagc atttttcata tttgtattcc atttaactct tcatattcag ttagccagtc 1741 cagtcgatta aacccaattt cagaattatt accaacgact ccggagagat aatactgttc 1801 cttgccttta attccgtaac tagatccaag aatatgtaca tagctagttt ttaaactttt 1861 tgccttttca taagcttcct tcgccagaaa atgatcagga gttacgttga acatagttat 1921 cgttgctccg gatcaataaa gggcagttct tcagaatttt gcggacattg tgtctttaaa 1981 attaactcag ctttcattgc ctttagttgt ggcaaggtat aggtcactga tccagatttc 2041 catgttagtt caatacttac attcactact ttcaacatag gatgatctgc gtgtccagtt 2101 gcataagtat tttcgagttc ttcgattaaa acttctatct gtctaaggtt aagatccgga 2161 atagaattta agtttaatgg agctttattc atgaatattg ctccattgtt caactacatc 2221 tcgatgcgtt ttctttataa aattagtctc aacacggatc agttgagctt tcttttcctg 2281 tatatgttca gccttaaatc cgtatttcat atctatcttt ttagaaatgc gtctaacttt 2341 ttcttcagca agtttctgcg cttctatatc tgataattct ttacggatca gaatatagtt 2401 agcaggaaaa tcttgaaccg tttttccctt aaaagaccaa taaaaatcta gagtatttaa 2461 aaatgttagc catgaaatct gtgtattagg atcagtgaac atctcaagtt cttcgtccca 2521 atctaaactt gttaagtcat tggccataac agttttaaat tgttttagta aaagggtatc 2581 taagttagaa tcgctctgca taacttctct ccggttgttg tgtgacacat acaggagctg 2641 caacttctgt atgtgtgctt aaattaaagc tattttgtgg ccccaaaatt aggattagta 2701 aatatccagc actttaaaat tcgatcttct ttcggtggtt tatctccgta acctggagac 2761 aggttgttta cttcatcggc aggatgttta ctcgtacgta caggcttatt agcttcaata 2821 aacttatagc gtttactggt acgaagtaga ttccgcattt catttatttc tggtagagcc 2881 tgaaagtttc gtgcagcaac tttatagatc tcatttaaat tgatagcgat atgtgcatcg 2941 cccgttttat agtgattaag gtggaagctt gaagtacgga cagaattaag atattcaaat 3001 gcatcccaaa actgctgaac catcggatga tctgctgcaa tttcatcaac tcttcgtctg 3061 gccatctctt ctaaataacg cttggcatcg caaatatcag atagatccat gacatcttta 3121 aaaatatgtt ctgctattgc atcaatcatt gctgatactt gagcatgaca gagcgcaata 3181 cgtgtatgcg taataccttt ttgatgaaac tcgatttcta tttctctatg tttttcatca 3241 taagtcttga gaattttttc ctcattcttt aaacaatacg tcatgtatgt acatgcatct 3301 tcaagactca tctgagtgag ccgtgtcgca atatgctttt tatcaaggct atgatttttt 3361 gtatctacag agatatgcag cgtacgcgat agaatcgctt ctgtggcctg tatcggcgta 3421 ttctggctga tcattatggc tgcacggaaa ggtggctcat acgtctcatt accagccgtt 3481 tttaagcctt tagagcgaat tgctcggcca ttgtatgcat ctttaagttc gtcccagcta 3541 aacttggcct tctgagtgcc ttgttgatcg ttccggtcac cttcaatcag tacgataggc 3601 atattagagg tttgagcaaa attacggtaa attgctacgc ttgttgactt gttcggatca 3661 aagccctcgt aagcttctcg accactcagt ttccacaaaa attcaatgag tgttgattta 3721 ccactaccag cttgcccaac aaattcaaaa aaaggatatg aggagttaat agcgcggatc 3781 tgttcagcaa aatatgatcc tgtccaccaa gccaacaaaa tcagcccctt ttcaccctga 3841 agagaataaa aatctttcca ccagtctggc ttaaattctt ttttagggtt taaacgaata 3901 actggagagg aggcgagtgt tttcacttca atatttcctg ctttaaaaaa atcatgttca 3961 tttttgggga taacttgggt tttattcaca gcgtattcat caaatatgta agtcttgtac 4021 tcttttgaat aaccaatgaa gtcaatggtt tttacttcac gtaatttttc agttttacgc 4081 ttgatgaagg tttcaagctg ctgatcactg ccggtccaca ttgctcccga gagcaccgac 4141 ataacgcgag gcttaaactt gctacgtgat gacatttgtt ctgctgtgaa tgttgttttg 4201 acttcgcccc aagggctatg aagatgaaag tagtaccaag attcatctgt aatttcattg 4261 cgctggaaat aaagtggctc tagctgggca ttacagattt cttttgctga agagcaggtt 4321 tttaaagctt gtatccggat ctcttcatca gttaatacct gatcttgctt gctctcttca 4381 acatactgaa cagccttatt gaatttttca taatccaatt cccaccagta agtcctaaat 4441 cggtggttaa agaagaattt acttaaatta ctgccgtaaa aattatagat cagtaaccct 4501 gcttcctctg gcgtttcaac aatttgtaat ttgccataat ggatataggt ttccagatgt 4561 tctttagtga gctgttcacg ctggtaaagg tcgttccaat caaaagattt accgtttacc 4621 ggtgcaggag gaagggcagc ggttgagatc cagccttcat ctgtcgcccg tttatgatgt 4681 ttagataaag cacttttgcc ggccttatcg ttatctaggg cccatactaa acgaggttta 4741 tctctattta gttcatggca gcgtttttta atttgttcca ggagaagagt agggtaattc 4801 ccactattca tgatactgaa gctaataacc tcagattgag aaaaagagat actgtcaaag 4861 attccctcgg taatccagat actaccagca ttacagagtt catctaattt atgtatagtc 4921 caagctaagc ctgcccattt cccgatgaag tttgctttct gccggccgaa gcgttctggc 4981 cggtcaatca gacgttccca ataaattcct tcagcaagct taaaacgcac ggtggcacta 5041 atcaggctgc gatttttcgg agactgatat aactcttgcg agtaaagccc tttgagaggt 5101 tttagatcaa aaccacgcgc ttcttttaaa tatgcatctg ccgcagcatg tggattttct 5161 ggtgttttcg gatgatattc tgaccagtcc ttaaaaagtt cttcacatat atcttttaca 5221 tgctcttcat aaccgcattt atttagtcga ccacatttga ttatgcgtgg tgtttctgca 5281 tgagtataaa gttctttctc attacatttt gggcacctgc cttcacggta ccattccccc 5341 tttcttttaa aaccgaaaag ttgattgatt ctgtcatcaa tacgacgttg aagatccatt 5401 cgtatgcctt tagagaagtt taatttgttg aagtcgtctg cggtaaagcg aggcccgtgt 5461 tagatcaatc gttgatgaat tgaaaagagc tgtaatgtag ccctcgacat aattcttctg 5521 gtgtagatca atagtggttt gaatctcaag actctccaac tgatgctcca gttccgtttc 5581 ccattggcgt ttatgctgtg gaagaccgat ctgaacctca gtgttaggat taatactgtt 5641 ttgtacaggt ctgaccattt ctaagctggc tgcaaagctt gctaagcact ggtcattccg 5701 gcatgaaaca attaattcat gcaaaagcgg agaaggcttt ttacggcttg aaacatgcat 5761 gcgtgtatta cagtgtgggc agtaaaaacc tatagattta ctcatcgttc aatattccta 5821 gatatattaa ttaaacgact tggagcaatc acatttgata actgaatgtg aaaatgaacc 5881 aaggtcatgc atgtcagctc ccgacactca cctacataag cacgtggttc ttcctttgaa 5941 acaacaatag tccccaagac tgttaatggc tgttcacaat taggacagca aaaaaaactg 6001 ttttttattt gaagttgttg ttgaatggcg ctgtttggca tgaaaactct taaacctgtg 6061 ccttatgatt cttttaattt ctgaatttga tctaaggagt cagtgttaat tttattcagt 6121 actacttgct ttgcgtactg gtttggagag agagaactat caagagcagc ttgaatcatg 6181 atgtccttgt attccttctc agtgacacga aaagcgattt gtttgtctga tttattaatg 6241 ttttcagatg atttcattgc attgatctct taaaaaatta aaatgttgga aatcgtacgc 6301 tgtttgattt cgtacgctgt ttgatttcca gcaaagacaa aatactactt ttaaaagtag 6361 tattcaaacg gtatttattt ttatgaatag tttttatgat gaaggacgag gtaatcgtct 6421 gaaagatgaa agaaaaaagt ttaacggcct tacacaaaaa gcggctgcag aaattgctga 6481 agtaaaagaa caggcatggg tacgatatga aaaacatgga gcaccattcg atttaaaatt 6541 cgctctgctt ttacaagagt atggcttcga catgatgtat gtaatttttg gagtaagaaa 6601 aacattaaat cccgaacaaa ctcagttgct cgggattttt gaaaaattag atgacaatca 6661 aaagcaaaaa gctttacaaa tgcttgagct aattaaatga ttcgtttgcc tcttcgatat 6721 tacttataac tgtttccagc tgctgattga tttcaccaaa aatcgttgcc aactcagttg 6781 aagtaatatc tggtgaggta tcaccgcctt ttagatttaa ggccagaata aaactcactt 6841 tgtttttcag ttgtcttaat tcatacaatt cattttcatt aagtttaaac atttgaatat 6901 ccttcaatta ttaattttat acggttttga aaccgctatt aatgagatcg gataattcat 6961 tgaaaaattg ccaacttctc tcagtatttt caagaattaa tattgcttgt acagaagata 7021 tactcaaatt gtatgtttca aaacgttcat tgtagtgata gtcaatattt tccctcaatc 7081 gtgaccagac tatatttgtt tcaacaaaag ttttataggt actctccgga tcaagcaggt 7141 tatacagttt cttactgctt acaaaaaact ggcgtttatt attcgccgga ttttgaactt 7201 tgataataat tgagccgatt ttcattttgt gttccttttt attattgaag tatatgttga 7261 ggtaaaattt gttctactgt gtgccattgc cagcgattta tatcgagccg gctggatgca 7321 gaggccgcat aagcgagaac ctctcttggc cgatctgcaa acttaaatat caatggatca 7381 tcatgagtaa tatcaacttt taaacctctg agctgtctat acaaagcacg atgctgactt 7441 tgtagcgtcg cgagatcctc tgctgttgcc tctggacatg ctggcgtatc cgtaccaact 7501 attttttgat tattaccctt agtagctgct ggtgctgaag cttttacttc aacaccgata 7561 ccgaataatt cctttaattt tttataatta atagtgtagt aattcacacg catagatttg 7621 tttggatcaa gcttgttgat ttcgattaaa ccgagctgct ttagctttga aactgcacgt 7681 ttaattgatg agacactaaa taaaccaagc gttgaaaccc actcttcata agtatgaaac 7741 caccacctcc ggttgttgtg ctgagtaaga tttttcttat ttttatactt tttggtttca 7801 ttcaataaga aataaagctt ttgaagaaaa gttgctgctg gcatgcccag ctctttagcg 7861 agctggggcg atgcgatgat gggatattca ttcgtcgaaa gtagaacttt tttcatagtc 7921 caaaacctcc gcgataagtc tctacataac aagccttaca acgagcagtc cattgctgaa 7981 taccgtgttt aaagccgttt ttatagaaga actcatcatc gaatggaaag tattcattac 8041 agcaagtaca acgcttttca ataccaagtt cggtatgtat ataaatctta gacataacgt 8101 tgcctcctag cgtgtacgaa ggcgaatgta atttaagatt tgtttactaa gcgaaggctg 8161 agcgaagtca gatgctttta agcgttgaag acaaagagtg tcgaggcata caggtgcttg 8221 atgcacacta ttattttgag tgttcatgtt taagtctctt gtagataagt tttaatacct 8281 accagcattc tttccacaga atggtggcag actaaacagg ggtggaaata ccgctctaca 8341 agtaacggcc agccttgcga ctgccctgcc tagcctacca ttacgcagac ttaaacagtc 8401 agacaaaaaa aatgccgcat aagcgacttt gattttgcgc ttgtagataa taaaacaggt 8461 ttccacgcct gatcacagat tttgctatga cgtggttagt attaatttaa ttattttttt 8521 tgtcaatcat tagattaata aaattattaa ttaataatct aatgattcga taatattatg 8581 atttttaaaa acaggatctc tagagaatta aacattcttt tataattcta atattcattc 8641 tatttatata tagaagataa tctcaaactg aaccaatgca tcggatcaag atgaactaat 8701 gcatcggatc aagatgaacc aatgcatagg gtcattttta gaatacaatt tagtatcttg 8761 ttttgactaa aaatttataa aaggggttgt ggtggaaact ataagcgtaa ttgcagggat 8821 catattgttt gttgtttgga tattcatgat cattggctta attaatccaa aatggattca 8881 attaaaaaat agaaaagaac tgacaagaaa aagaattatt ttattggggt taggagctac 8941 tatattactg acaatcatcg gagtttcaac caatccctct gaagagaaaa taccaggaga 9001 aaatattact agttctagtc cttcagaact aaaaaataat gatcagaatc catcaaacga 9061 agttaagcaa gaagctaact tgggtatgac tcctgaacaa ttcagaagtg attttaataa 9121 acgtcttaaa accttagatg ttcacatgat taggccatta gccgagttcg atattgaaaa 9181 aggagaggtg cgagatattt ttcaagttcc tttttccaat cagatcaata tgacaggtac 9241 agtcaacaaa gatggaatgt tacgtgaaat tactttcatc atcgtgccaa atgaagactc 9301 tgcacaagta atgctcgata ctcttactct aatcaataca gcttcaaata ctgttaattc 9361 aagtgcagat aaaaatataa ctggaaaagt tgttgtggat ctagttcaga aagctttgga 9421 aggagcagat caagataata acagccatag ccaagtggtc gggaatataa aatattatgc 9481 tctagcaagt aaatatacgg gcttgtggtt cggattgtcg ccagttgatg ataagtaaaa 9541 gattgaaata aactagaaga tataaaaagg gccctcatca atgagggcct tttttatagt 9601 ttattatggg gttaaaaatt ttattaattt agatagctca tcattaagat gagtgacgca 9661 aatttttgca taagggtttt ggtcatctgc cttctttaac tcttcaattc gttcatttaa 9721 catttcaata tacatttggg ttttttctaa atctttcatt ttaaacaact tagtttggtt 9781 acttgataaa agtattattg ctctctcttt gcatgaaagc aagaaaaaat aagacaaatt 9841 tgtcttaaat attgcttgca agtaagacaa aaatgtctta ttattctctt atcgtttaga 9901 tggagatgta catgaagtac agcgaatttg agcgatggct gagacaacaa ggtgtcgaag 9961 tggtacgaag tggaaagggt tctcaccgaa tagttagcta caagggaaag aaaacaacac 10021 taccgtttca caaaggcaga gaaattggag aaagccttcg caaagaaatc attaaacaac 10081 tagggcttta agccctagtt gttaagtgtt aagacttcaa agtacatctt caaatatggg 10141 cactttatat ggtgcaaagt cttatgcagg ggttttcctg aaagataata taagagaggt 10201 aagggttatg cagtatccag tgactctaac acatgataca gatggttctt ttttagttcg 10261 ctgtcgtgat ataccagagg ctttagctgt tggttactct gaagaggaag ctttagctga 10321 agcaataaac ggttttgaat ctgcgtttat gatctatata agtgagcgta gaactatacc 10381 gctgccaagt aagaaattaa aagacgagta cttagttcgt ttgcctgtgc gcgtgagttt 10441 taaagtggct ctttataacg aaatgttagc tcaggatctt aaaaaagcag atctcgctcg 10501 tcgtttaaac tgggatcaga agcaagtaga tcgtttattg tctttaaagc actctactaa 10561 acttgaatca cttgaagagg cctttcatgt gatgggacgt gaggtggatg tagttgtagc 10621 ttaatattta atagctgaat aaaaaagccc tctgaatgag ggctttttta ttgctgtaca 10681 cattgaacgc aaatgcttac tttaatatca tgtgccgtac atccggagat gaaaaaaatt 10741 aatcctagag ctaatagctt cataaactat aagcctgctt ctaaatctag ctgcgttgag 10801 agtccagcat ttttgttata ggtatgagta gctttttcaa caatccattt tttctcatca 10861 atttcttttt taaagccatc taactttact ggtgactcgg tactgattgc tggtatgcca 10921 taagctgaaa ctatactgaa ttttgccatc tggcgtttta tttctgccat tttagctcgg 10981 ctggcacgtt ctgctgctgc tttgttcgca aaggttcctt tcaagtgctt aacttttccc 11041 ttttctcctg taagggcctt ttctcgttta cccttgcttg tgtcttgata gcttgcagtt 11101 acgccagtat ggtcagactc tctatcctgt tcactataac gaaactggtc tccatctcta 11161 cgtgttaatg taattgcagc taaatcttta cctgatgctg tctttgcggt acctgctttg 11221 aaaattagta aacgatcttt tttgactgcc atttcagcac cattttgctt ggcaagtcgt 11281 agtatgagat tgaggtctga ctcattagtt tgatcaacat gaactaaatc aattgtcttt 11341 aaatcatctg tcatgactaa agataggtcg tgtttttttg caatttcacc agcgatctgt 11401 ccaaaatctt tacggtgata agatttagac tttgcttctt tgatattact tttaaagtta 11461 gcactggaag cttttacagt gatgagatcc ggagtgcctg accattctgt ttcatcaaca 11521 ataaaatcgc ccttatcatg gattacttca cctttaaaac ccatctgaca gtttagcttc 11581 actccacgtt tgggtaactc taaggcccca tcatggtcat cgagcactat acttagctca 11641 tctgcttcat ttgctctatt gtcggtcact gttacgctaa gaatccgctc agcaataagc 11701 tgatttaatg gtctggcatt ggcagtaata atacagactg gtgttttaat catgctgtat 11761 tttcctgttg ttgaggtgct gtctgagttt ctatccgttc atcatctgtt cttgaaagct 11821 ttaatgtgta atcgactttg cgcggaaccc cattatcaat aagcacagac ttggtttcat 11881 ctatgctatc gattacatag acaccgtata gataaccaga tccatccatc agaacaaaac 11941 cttgccccgt atctgccata tttgccaaga catccagagc gaaacgggta ccgaaaccat 12001 gttcttcata aataagtcca ggaagtgtga ccgtatcact gcctgctccc gtgaactgtt 12061 tttttggtcg accaaatgcc acagcattat cagaataatt ccagttacgt tgccgctgga 12121 tctctagaaa tgccaaagtg ctagtctgaa aaataaactg ccctaaactc attagcatga 12181 ttaaacccct aatcaatatc actataaccg cgaatgttac ccacacttgg cagtcgctga 12241 gcttttgcaa cttctatagc cactagctgt gctagagcct tctcatccat gtttggtgca 12301 ggatgaatat gaaaatgctg aacgattggc tgtgctgctg ttgtcgaact tttattgcct 12361 gcagcaattg aaggtcgtga atcaaactta attgaaccag tatcaaaata gcccaacata 12421 tctttacttg cggttgtcac tgcagtattg gccagatggc tgttattgga gattccgttt 12481 gctaaacctt gcatgttata tgcaccaagc tcagcaaaaa cgcggcttgg cgaatgtata 12541 ccgaatgctc ccttcacatt tgcaattact gcccctgcag ctgctcttgc tttcgctaca 12601 actgcaccca cactattacc gataccgttc gctaagccct gcataatcat ctggccaaag 12661 cctgtgaaag tgctcggaag ctgaatgccg aaataactca ttaccgatgc aaatacacga 12721 taaaagagtc ccagtagcga ccagttaaga atagttgcgc tgatatttgc aatgccgctg 12781 ctgaagaaag cttttaagtt ttgacacaag gttgctgcac ttgaaccgat accattccaa 12841 agacttgtaa aaaacagttt aatcggcgcc caatttcgat aaattaagta cgctgcaaga 12901 gctaataaac cgatggccaa gaagagggga ttggtcatca tcaaacgtcc aaccatgaaa 12961 agacttttac ccagccacaa aaatgcgcca cacattaact ttataatatt gattaatccg 13021 aacccttttg cacccagcac tcccagcgtt acacgaagca atgccatagg gccaagcaac 13081 gttactaaca caagtgatat tgcactgaag agagctaata gaatcacgat ggcagcacca 13141 actttcataa tcgtattaga aagctgtgga ttagctttcg accaatcccc aattttttct 13201 gatacatcag ttagtccggt tacggcctgt tttagctcag gtgcaatcgc agcaccaaag 13261 ttagtcatcg cactggtgaa tgtgccagca gctgcatccc aaagattttt taaagtacca 13321 agctgctcat taacacgtct ttgtaagtct gcctgagcat tcatttttgc taatgtttca 13381 ttgtagccgg ccttgccttt gtcgattaac agattcaaag cttggatcgt ctctgcatca 13441 ttgccgaaca tatcagaaag tattggtaat ctagcctccg ttgataactt cctaagcttg 13501 ctgagctgtt taaacatatt atctaaacca ccgaattccc ccttgccatt tgtgaaattc 13561 attgtaagtt ttgagccttt taatgctttc ttgatgcctt tagtgtccat catggcagca 13621 aagattttac tgtatgcatt acctgcagac tctccagcca ttgcagcctg atcagccatg 13681 acaagaagag gtgccattgc ctgtgcacct tttagtcctt cagctttgat ggttttcatg 13741 ccggcagata atttggagaa accttgcagc atgtttgttg aatcaacacc caaatagtaa 13801 gaacgttgaa tcgtatccat taagctcaac atatcttttt ctgatgtctt ggtagcatcc 13861 tgcatttttg cagcaaactc cgcagcttct tcaaaaggca ttttaagttg aacagccaga 13921 taacctgctg cctgtccgac acctccaagg atggctttgt agctgatacc ttgctgcacc 13981 agcttcgcca tcatgagctg aaactcggct gttgttcctg ggagctttgt tcctaactga 14041 tttgcaagct cgttaatttc cctaaaatct ttagctacct gtccattaga ttgcatcatt 14101 gaaactctaa gcgtcgttgc agcatcttca gcttcagcaa aggctttaat tggtacactt 14161 aatgccccag cacctactgc cgctcctgca ccagctcgca tacccgtatc ggaaattttg 14221 gaaattattt cctgagtatt tttctgttga ttgattaaat cattgatacg cttttgactt 14281 gcaatctgtt tatctagctg ctgtttctta cgttgtagag actggcttga tgcatcaata 14341 cgcgctttta attcacgctc atctctaccg agcgtttttg tacttatacc agcttgattt 14401 aaaccattac gcaacatttg caattgtcga agttgatcct gttcagcttc ctttaatgat 14461 tttactgcta gtttggcttt ttcaaattcc ttgatcattg cccgtgtagg tctctgtaca 14521 ctggcatgct cctgagccag cctagcaacg gtctgctggg ctgattcaag atcactgcga 14581 gtacctttca atgcagtcga taaacgtcga aatgacgtta tatctgactg cgatttacta 14641 agttttttta actcttcacg ttgagcacgt agagcgtcac tggccttttc acttgaaccc 14701 ataatatttc taagtggggc agtggcctta tccaccagct ctaacatggc ctttaagcta 14761 atctgactca tttgttttgt gtttcccatc gtgcacgggc acgttcatgc caatccatca 14821 actcttcaat ttcaaagtca tcgcatgctg aaggcatcca gtgaaatacg atggccagat 14881 ccgcaatgac ttcgtcagta cttactggaa gtcttcgtcc tccgctctca cgaaaaaact 14941 tacaacttcc ttagatagtt ttgttaaatc cacaggatct agttcataga catcattttc 15001 tgacatggtc ggtgaagtaa tacgtggtgc aagtttagcg agcgtttcta catccagttg 15061 cagtaaatta actagagtta agccacgtaa tgcgcgtgtt ttcggtttac ggatggtaat 15121 ttctttaatg aactgttcac cgcgtttaaa acccacatca aactttacag gcttaatatc 15181 tggatcttga attgcttgct gattttgctc ttgtacttcg gtgttcataa tttttctcaa 15241 atagttaatt gaatagaggg aagtacttga tctttacaga ccaagtaatt ttttaagttc 15301 ttgagcgagg tcgcctgagc caaaacgttc tgtaccatta atgaaatcaa gctcaatctc 15361 gactacgcca tcgacttcca tgcggtaata gctatagttg taggacattt tgatttcggt 15421 cttttcccca ttttttgaat cgccaggatc tagctcgata gcctgtccac gtgtataaat 15481 ttctactgct tgcgtagtgc agttatcctg gcgttcatag acaccaacaa agcgcacggg 15541 taagtcagaa accccacaga caccgagctg tcgatattgt ttgacatcaa aaccagcata 15601 ggtgacttca ccttcaagtt tttcatagcc catcgtcagt gcaatatcac cgatcatgcc 15661 tgcgccacga aaatcttccg ttttcttggc tatcttggga attgtgacgc tctccgcgac 15721 accggcccac gaatgcgtat taacaaatac attaaaattt ttgagcgtac gtggaagcat 15781 tgattagatt ccttctgtgg ttacttggcc attaatcagt tgagcgaaat caactaaata 15841 gcggtcagta atacgttggt ttaagccgag gttctcaagt gttgggactg gtgtgtagtc 15901 atagtcaatc cacatctttc cttgagataa ctgttctaca gagttttcag ctgcgtcata 15961 ccagactgaa gcaccgatta aatgtttagc atcaacatgc tcacgtaact tggcatttat 16021 tgagtcgata atgtccttag ctaaaaaagg tgtaagtggt tggtcgacga aaggaaaaca 16081 gccctgaatg atcgtatcga gaagaaactg tgctgtacgt gtcgccactt caaatgaaaa 16141 gcggatatca tcagaacagt tgcgattacc ccaaaatcga aagccttgat gctggatcat 16201 ggtggtaata tcttggctgt ttaaatagcc tgcatctgta tctggatctt ccagatccca 16261 tgttatgggt tgactaatac ctgtcggtcc agtcacagga atattagaaa gtgatttgtg 16321 aaatcccact ttagagtcgg tttcagctcg tagagctgct gccacgacta ctgcagtatg 16381 tatgccgtag ccatataagc ttaattcagc catatttagt ccttgtatta cgatggttga 16441 tcaacgtgga tgatgtctac ttgtccgaga gtcgagctat tagtgacgat ttcactgccg 16501 ctcggcatca gcacgtagta gccattaatc agcggtgaat agtcatgggt aaattcgatc 16561 tggccagacg tatttactga gagaagtttt aattcatttt tgtctgtcgt tgctaagaca 16621 cggccatttc ggggagtttt aaaccgatag tacgtatcgc caagatcttc ttcatttcgt 16681 gtcacaagat taaggctatc tgcatcgact ctaataacat caacaaagcc tctgaaatgc 16741 actccttgag agctatcctg tgaatagctg cgaacaataa tattgttgtg caaccaaact 16801 ggagggctaa gagttctgag gccccattcg ccactatcgg ccatgcctaa tctgttgccg 16861 atctgcgtag caacaccttg gctaaagcta taaactttta agtcggcatt accggatgtc 16921 acataactaa atttgtcacc ttcttgtgag aaagctaatc cggtgaaata atcctcttca 16981 gcaatctcac ctattttttg ataaagcgta gatgtctggc gttgatagat ttcaacattc 17041 tttcccatag ttcctgcaag aatataactg gcatcaggat tcacatctaa aaatgcagtg 17101 tccattaatg gtggattgaa ggctatttca agatcataga atccatcggc agttcgatac 17161 aaagagaatt tattgctgat taagactgaa ccatcggctg aaaatctaac atcagaatca 17221 tgatcaagga cttttgaaaa gatattctgc gatgtatcaa cctggctacc gtagagtgga 17281 tatacgccga aacccgaatt attcgttaaa aatccgtaca gatatgcccc atcgtgcgtg 17341 acattcaatg gagaaaaact gcctgaagga atcgtgattg gttcaccagg tgcaagacaa 17401 acttcaagta tgcgtttatc actaccttgg taataagttg gattcttact attgttaggg 17461 ggcagtataa ttacatcatg gttatcatta ttttcagtga agtctacttt caaccggata 17521 gtctgattag aaatattttt cagctttgtg cttccctgat ttatataacc agctggcaca 17581 tcagtagctg aagtttcatc agcatctgga ctgagcaaat gactcaaagc tcctgacgtt 17641 agatccgtaa atttgtgttg aacaccgttt acaaggagtt ttgcagataa aatgatcggc 17701 attagttcag aatcattttt atctaatttt ttacatgaca ccattgtcat ttcgctggtt 17761 gcccctgcac agctaatagt tcctcctggt tctactgggg ggcttgagac tttcccaaaa 17821 aaacattacc acttgtaaat tcaggccaga taatttctac ttctcgaaat gcaatttttt 17881 gacgaaaagc tgcaacttct tgagccgaat ttaaaatctc tccagtatta ctacgtggcg 17941 taatataaga ataagcacgt aactttttac agattgcacc tatggcattc gtgacatcca 18001 aggtttctgt atctggaacg cagataattt ttggtgtaat tccaagcact gatttaacag 18061 tcaaagcagc ttgtagtcct gtgcgtttac ctgaagaatc cgtggtacca attactaatg 18121 attgattgaa ctggccattc ttgaatgggt cagcaatacg tataacgact aatgtcggtg 18181 agccaattgc tgagatgatt tctaggtgtt tacgtaaatt acctgtcagg ccagctttag 18241 gtaaagcacg attaatagaa gtgaccaaaa ccggcgtgtc aagtggaaaa gaagcttcgt 18301 cagcatcatc agctattgct acaagcgtaa ttgtgctggt gtttgcatca cgaaacggga 18361 tattaccgga agctgattcg cggccactaa taccgtgatg aaattcagac attttataac 18421 tccaaaagtg ggttgtactt ttagagtcat aatgtattga gtggttttaa tattctatta 18481 gcagaatcct gtttaacgct taatcaggat taaaaatttc tacaattgca aaacctattc 18541 cgccatctgt agctttattt ccccagctct tccagccttc tcctaattta ccaatatcaa 18601 tactggctag tattttttct tctgtggtat tagtaaacat agcacgtata cgtgctcctg 18661 agccggcagc tccaccgaaa gaatgacgtt catcaccgat acctactgct ccattaccgc 18721 caccactcat ttgcccgata atagctggta gcgcacttgc tcctttttgt gtttcccaac 18781 gtgaatatag aatcgcatct ataccgttcg ttatctctaa ctcatcaaaa atcgcttccg 18841 gatcattcac tgacgtaata cctccagttc ctggcttacc ttcagtataa gaggaaccat 18901 tcccccacac gccacctgtt cctgccttac cgcctcctgc aatgagagtt gatgtgccga 18961 ttgtgcaact tacatcccca gcattctggc catcaggttc ggtaccagtt cccgaataaa 19021 ttgagccacc accgccccca cacgggccat gtaaatgaaa tttaactgtc tgatttggaa 19081 gaatttcaat ttgatgtgtt ccttcataat aaacggaact gccttcagga tatttgctcg 19141 tatccttaat caaaacactt acttgtttat tttttatata ttttaatgag aaaactaaat 19201 tttcatcacc ttctgtttta ttgtcttcag tgatttgtat gctgtggact gctttacctt 19261 cagtatctac aacaaattgt ccagaaagga tcctaggaaa aaaatcatcc tctttgatgc 19321 ctagccctgt aattgtccag tcaacaggag tgccggcatt taaaccggtt gttatcaaag 19381 taaagataac ttcatcgcct tcattcgcta tggatttatt cacagtcaaa gtatagcttg 19441 gaggagatga gccatctggt aatctttgcc agatccgtgt cgatgatgct tgtctcgagg 19501 agttaccgat ctggagttca gatatggtcc caagaggatc attgactgat tcaacccctg 19561 caggaatgta tggccagatc atccatgatg ttctatagcc aaagagtggt tctaagaaca 19621 atgaaggatc ataatctttc ttatttgacc catgccaaga ttcaacatga taaatggctt 19681 ctactagaga attaaatttt aactcaagtt tagctacttc tttttctacc cattttttat 19741 ttgcagtaac gatatttgga tcaatctgaa ttaaaacgtt cgtactagta tttgttttaa 19801 tatcaataac aatttcaagt tccccagagc caccatcagt aataactggc ttataggccc 19861 catgataatt gccgatatat accaattttc ctgaagaatc tgtcaggcca atttcatgaa 19921 tattaaatcc accaatttct gaatttactg tagccgttac tcttgtgact gtatcaatga 19981 tttctactgt ctgaattgga acttgtgcac actggttaat taaacttgtc agattttttt 20041 tatctaaagg atcataggga ataccatttg catccccgat aactaaataa gcaagagcaa 20101 ttgaactttc ctgatgggcc ttagcaataa gctcagcacc gtaatcagtg agagcgatat 20161 aatattttgc tgtcataaaa atctctttaa atacttgtta gatgaattct ggtacctgaa 20221 gttacagagc cggtaatgta aaaattacat tccgttttaa cttttgcaga aattgaatag 20281 ttatcacggg cagattttgt cgcatcaatt gcagtgtgaa cctgaagcaa ttgttcaatc 20341 gaaattaagt gcttcggatg aataatgact acaaaagtaa atggctgctg aggtggcaga 20401 aactcatgcc aagcttttat ttgaaagttc tcgactactg tactgagcaa gctggcaata 20461 gatgagcgtg tgccacgccc agcattgaaa gacttacttt cactgatcgc atttcgcttt 20521 tcgatctcag tccagtccgg tctccagtat tcaactcgat gttcccatgc aagccaagga 20581 agatgatcaa cagggcaacg atcaatgcga tgtaaatcaa taaaaggtac ggcaaggtta 20641 gaaatttttt caccaagttt ttcaacattt ttttcaagtt ttgtggcatt cggaggcagt 20701 agtctataca tcgcggaact caacaatgtt gatacgaata gctgtacatt tcgcatattt 20761 attctttgtt agtacgatgt cagctgcagg gctgtctagc accacattct gtacaccgcc 20821 ttgatgcaaa gctctaaaca agccgctacg tgtaacgtca tagcctaagg aggatgtttg 20881 ttgagtatag agaagagcat ctgcttcagc agctgctttc acaacagccg agtctggacc 20941 tggaaatagt acaagtgttg catttagttg ccacgtttca ggttcagctg ctttaacatg 21001 cacccaatct gtagctggtc gacgagtatc cggatctaaa gtttggtcta cgatattcaa 21061 tagttcttgc gatgcaattt catcaagatg actctgaacc caaatattga catggccttc 21121 tttcggtgtt tccacagcaa tatccttaac atggccatgt gaactcaagc cccaaaattg 21181 atatgcgcct gtggagccag cagacatact ttcaggttgt aaatgagttc ttcgacgtag 21241 agcttcatct gattccatga cagcttcgac tggaggattt gcattaggct gagcagggat 21301 aatggttaaa cggtaaactc cccgatttga tgctaaatga tctaaatcgg ctcctgttgc 21361 ataggcaagc attaaggctt ttgcttttgc attcgtttgg ccagtcttta gtagaaagcg 21421 ataagcaaat gtttctagta gcttaaccaa tggctcactt tcaaaactta aagcttcaac 21481 cagttccgga tctcgtgaaa ccaattcatc cttgcaagcc tgtaattctg tttcaaagtc 21541 tacttgttcc aatacattag gaaagggaag agaggataga tcaacacggt ttaatgtact 21601 catgcagttg ctccaaaggt ataactttgc ttaaaaggtt gttcttggcc atattgttta 21661 tagctgccga tgagcgttac attccaagca ctttcaacct ccgtactgct tacctgaacc 21721 tgtttgattt taagtcttgg ttcaaagcgg ataatggctg tagctgagcc attcataatt 21781 tttagtttgg taatgtcatt acaaggctgg tcaataagat ggggtactaa tgaaccataa 21841 tctcttcggc atattcgagt accgattaag gtacttaaaa ggtcatgtaa tgattgttct 21901 aaatgggcag gcaactgccc tgaatcagtg agtttctttc ctgtatatcg agacatgcct 21961 ttcatttgct catacctcct gtgggagctg gcttctcagt ttgaccagtt cccttcatga 22021 cacctggatg cagatgttca atcagactaa tgtctttagc tttaacatcc ttatcagaag 22081 aaatgatgtt ttctgcatgc acttttcctt taaaagtggt ttctgcaaca tcaaaaatac 22141 atttatcaaa tttgaataca gcttcacctg aagagacttt aaaacttaaa aaatgttcgc 22201 cgatctggat tatcacttca tctggatctg tactcggagg agggttctct tcagagaata 22261 ggcttacttg tggtacggca ttagtaagct ccccaccttg tgcagaaact gagaattgct 22321 ctcctaacga tgggcatcgc cagattttga cgatgcctgc agccatcgtt ggaatgggta 22381 gccaatccgt ttcattttca tcaatctgca gacgcatttt cccagcagtg gcatcaactg 22441 cgatgaccgt accgatactt gccagatttt gaaattgccg tgaaatttga ttagtcatgc 22501 tcatataaac gtaaattgcc catgagcgaa atcatgggca atttatcttt aaagagcgag 22561 gagctgaatc ctgtttaacg cttaatcagg aattttaaaa ttagtaataa ttatctcttt 22621 tccgttttgc tcactttgag atttaacatt tagtgaccag cggatctgcc ttgtatggaa 22681 gtaaaagcct gcaaataatt cccgtacttc tggaacatca ttcaagctta aaataaattt 22741 ccccttaata tttctcagta aatcacgtaa tttaataaaa tcatcgcgtc cgaaaatatc 22801 tttgccataa tagttttcac aattgtagta agggggatca agatagaata atgtttctgg 22861 ccgatccatt ttttcaatta agacatcaaa atttcggttc tcaatattta ctttctgcaa 22921 acgttgattg attgctgaaa gtatatcttt cagatcacta cccaacttta atgttggtaa 22981 acgttcagca tgagagtaat aggttgggcc ctcaattttg gctccaaaac acgtacgcaa 23041 taaataatag aaacgcgctg cgcgttgaac atctgtaaga gatgcaacat tctgagcctt 23101 gagtcgatca aattcgtcgc ggctaatcaa ttgcgtttca aactcttttt ctaatgcatc 23161 aggatgatat ttaaagacac gatataggtt tattaaatca ccgtttacat cattaattgt 23221 ttcaagtgta gaaggggact ttttaaagag aacccagccg gctccaccaa atacctcaca 23281 ataatgttta tgctcaggca tcatatcgat aatggtacgt gctagttgtg atttaccacc 23341 tagccatcct gaaaatgatc gaccagaggc gttataagtt ggctttgtta cttctttagg 23401 gttagttttc ggggacattg ttgtcatctc cgttggtagc tctgggctat ctaacctgac 23461 gctctaggcg ttccaatgat ttagcactct gcaacgaggg catttaattt caagctttac 23521 tgtgattttt tcaatgcggc acagtagttt tccgcattta catctaagtt caatcatgtt 23581 gataagaatt tctgtatctc ggatctaatc catttttcgt catcattgct aaaaccgact 23641 aattcacgaa tcggatagct ggtggctttt gcgtttcggc ttggcttaat tgtcttacct 23701 tcctgatgaa tgctagcaat agttgcagta cgtccaccga aaccgactgc agcatgatct 23761 gctgagtatt cagttttaag ctgcttgcca atattctgga ataaggcacc ttgccgtttt 23821 ttactaccga tctgttcacg cttcctggga ataaaccgat taccacctgg atctctttgt 23881 tgcttaatcc gatctcgatg acgaactctt aaaccttgtg ctaaacgacg cataagctcg 23941 cgacgctgat caggctctaa gcgaactgca atctggtcca gccagtgagc taaaccacta 24001 taagcgtcaa tcattcagag cctgctggaa taaatttccc atgtttatcg ctccagacca 24061 tttgagggca gatatgtggc ccattttcat tcataacgac tttgtcacgt tgagggaaat 24121 ctatttccag atcaaaggta tctgtatcaa taacttcact actgaaagaa agtaatacat 24181 cttgaccggt agaatccaag tttaaattgt gagcttttaa ccagttacga atgagcatga 24241 tcacttcaat gggatcaccc cgaaagtcca ggaacaacaa tcgcgctgta tattcaagat 24301 atccctttgc ttgcgttcca ttcactatta gcagatgaca tttatctgct gatagtgtcg 24361 ggattttttc gctcagataa gacttcaaag ggattaaagc tttcatattt aattatccag 24421 ggcagcataa ttaagctgat gcgcgacgcg tatagtccag cctctgccgt aacgtggcca 24481 agtgcttaaa ctactcaaat gtaatagccg aaaagaatta aattttagta atacatcgtt 24541 taaatctgaa tcttgaatag ccttcaaagt ctttttacca atgatgccat cttcagctac 24601 attaacagct ttctgcaact tgcgttttgc agtacccata cctgaattga ttgcaaagtc 24661 ccaaacttgg aaaacaattg ctggatgaat ctcatcagcg gaaatacgat cccaccagtc 24721 tcgtcgatag atttgtttgg cgcgttctaa ggttaaattt ttaatatcta aatctggata 24781 ggtcattgct gaaattccaa atttagtacc tttgcattgg cctttaccaa taattcctgt 24841 cgtccagtta ccacgatctt taggatcatc agtaaaatta ccttcatgac cgattaaacg 24901 ttcaaatgct tgttcgaaag taagttccat gaaattcccc ttacttgaac attgcagtga 24961 atgcagcttt tacttctgca acaatttcat aaagtgcctt gcctctcatc aactgcaaag 25021 actggtacca aataccaatg attagcatgc caaagactgc aaaaatcagc attacaaaac 25081 cctgcgatgt tattgaatac tttgtgagtt gataatattc aatacaagct gatccaccat 25141 acaaactgat ggccacgcta atagtaaatt ttataattac ccctaaagta attttgatcc 25201 ggccttgtgt atctatatca ccgcttaaaa tcagagctag tactgcccca agaattgctg 25261 caataatttt taaaagccac ggtaatccct ttattgaaat aggatcattc attggtttcc 25321 ccttattaat cccagagttt tattgtggga gggctttgta tctgagtggc atcaggtaat 25381 aaaactgctt gatgctcatt taaaaatatt tcatgcagat caggatttaa ttcaatcagt 25441 gcagctaaca tctgcacggg attatcttta aaataacgat aagcaatggc atccaaagta 25501 tcgccctgta atgcatagac agtattcata ccagcttgac cctgttacgg ggtttgtttg 25561 tcagactagc aatggaatga ttgacaattc gacgcaatga ctgagctttt atttgctgat 25621 tctcaccgcg tgattggcca ccggtagtcg tatcaaaatc caaattttct tcacagatta 25681 gtgctgctgc ttcatagcag acagcacgtt tatatttccg gatctgttca tcggtttcta 25741 tttttagaag aagtgtctgg tcattaatat cgtccattgc cagaacaatt ttttcagcta 25801 aaagttcggt acctttggat aaatctaaac gcaccatgcc catcaagtcc ttgatactga 25861 catctggacg atctggctca ggatttttaa caatatgatc aggcacagat gcatttagaa 25921 gcatagctat tactccggag tggtgtcggt ggacgcagga actgattcat tctctgtcga 25981 aacagaagat gcatcagcct gcgagccgac agtggcgtcg ggagacgact cggttggctg 26041 tagattcaat ttcttttcaa gctgagtaac ttgttttaca cagcctgcat ttgtattgaa 26101 tcgtagcgca ttcttataag cgtttagtgc ttctactggt tgggcatcac gtagtgcatc 26161 accaagagca cggaaacctt ttgcacgaac ttcatcaacc atgctttcgc ctgtaccaat 26221 ttcaattatt ttttcaataa cactggcatt ttttacagct aattcatggt ctttaatgaa 26281 gtcttgagaa cattgttcag tgataaactc agcaatactt cgtgtatgtc cttcaggcat 26341 taccatttca ttaagtaatg cgaattcagc aatacgtaca gcctgttcat aatcaccgac 26401 atctgctgcc cagatcatta gacgtactaa tacgttattt tgtggagctg gtgaggtgac 26461 aagacagccc tctatgtaac cagcatattc cggcaaccat tctgcttttt tggctatttt 26521 ttcctgaata gagcgaatgt ctgcaagctg ctgaagctga ctgaacattc gcagttcaat 26581 attgccagta ggtgaatttt caacaggaac agttgctgag taatcacctt caggagatcc 26641 aggatcaaag cccatgctca tttttttctt gcgtaatcgt ggatcagggc tttgagcaag 26701 tcggactgct ttttcagcca acttgcgttc acggttttgg cgcattgaat ttgacattag 26761 gcagtgacct caatgttttc aatcaaaacg cattttgcgt agtcttcaac cacaaaatct 26821 tcattcacgc tttggaaatc tactgcgcga tcccattcag gttgttcaac aatagaacgg 26881 ttcaaggtgc cggattgcag atagatagac aagttatctg gcttagtgat aagaatcgtt 26941 tttgcaggga acttcggcac atgcaaagct ggcaaagttc caagctgttt attcgcataa 27001 atggtacgcg cagccagttg ctcagttgga tcttgaaccg tatttaatag gggcaaatac 27061 ttgtcactca agatgcctcg tccacaaatt actaccaagc cactatcacg gtgctgttca 27121 gctatatatt cttcaacagc aaactctact aatccatcaa ttgttttaaa ttcgctattt 27181 gcactgatct ttgtcaccag tttgccagta ccgtcatcta caccttcata gtgttgttca 27241 ggcgcatttt cacgaatttt ttgcagccag ccttttttta catcttgtaa aagcggattg 27301 gcaacacggt cagaagttgg cgcacgatat agaccattaa aaccgatgca aagtttatcc 27361 agggcgattg ccttaatcac catagcctgc aattttgctt tgaagtctgg atgatgtttc 27421 caagcattca gtaatgacca gtagtacgca acatcataat tggtctgtgt acattcatac 27481 tcatcaagca agtcaagtga accaacagca actggcgaac gtggagtttt tcgggtatct 27541 gtggtaccgg caacggtagt cccaatttga agaccaattt tttccgctcg tgcattatcg 27601 acagggaaaa tattaatttg ttttaaaaag tcagcggact cctgataagc cgcaatgatt 27661 ttttgttcag gggccggctg aactgcaaat gtgtgtcttg catcttcaac attatttgcc 27721 cgcgcaatgt cagtaatgta tgaagatagt tttgtacggg cgataggatg taaaacgact 27781 gacataatta tagtgtcctt aaattagtat gtaacttggt ctgcatcacc accagtttgt 27841 gctggtggat tattcactac agtcgactgg atctgtttaa actgattttg cagaccagta 27901 agagcttcca tgactggggc cattacctga gtaaattgct cacttgaatt tgccggtact 27961 gttgattgag tgattacttc agctggagct ggagttgtgg ctggagctgg tgtgattgat 28021 gcttgcaaag tattaaattt ttcagtgaga tctttaatac cgttcagagc tgttactaca 28081 cctttagtaa ctacttcttg aaaatcatca ggctgaggtg gagtttttgg ttggcctgaa 28141 agtgctgttt ttaactgttc aaaaaagcct ttttgttctg tattagcaga attattttgt 28201 tccgctggag ctgccggtgc tgacatgaaa atctccgtgt tagggtgggt acgtaaggaa 28261 aagctatttc cagaaaattt gatgggctgt gtgtttaacg acgctggagt atctgtaagc 28321 ccaagaccga cctgataagc cagacctgta ccagcgaaat tgcgatagaa ctcaatagat 28381 gggtaaatct tctggccctt tttatttagt tcaataaaat tcggcagtgc agagatggtg 28441 ttataaagcg agtacttccc atcctcttct acggcttcaa ctttgataat gtcgccatag 28501 gcattaaagg gtggttcagg tgaccaacca gagaaatgtt caaggttgat tcgagcacca 28561 tatttttcta tgctgtaagt agcagccatt tgctgaattt cctctcgagt cagctctcgg 28621 ccatcaacag tttgcccctc tcgtgcgaca cgaaagcgtt tttcaacacg ccctgcacct 28681 gcaagttcca tacaaatttc catctatgct tctgatgtat gcagtctgct tgcgagaggg 28741 tagttgctgc aatgagaaga atcctgttta acgcttaatc aggatttttt catttagaga 28801 aagcttttct ttggggcaac attcaatgta tgaatacacc tcaaccaatt caattcgacc 28861 ttatgaatcc tcgtcaacac ggaagaatcc tgttcgcgat ggggatgtct gtctctgaaa 28921 tagcaaaaca aatagatgaa aaacgggcca cagttgaaag ctggaaacag cgtgatggct 28981 gggaaaaagc tgatctgttt gacgatgtaa ccttgggctt aaaaattcgc tatatggccc 29041 tgacttttat ggaaagtaag ggaaataatc actataaaga aatggatttt attggtgttc 29101 aatttgaacg ctgggcacgt attgaacgct tccgcaacgg tggtacgcaa gctgatttaa 29161 atccaaagat tgaaaataga aatgacaagc cacgcaagaa aaagcttaaa aatgagatta 29221 ctgaagagga tttaaaatta cttgagcagg cttttctgga tcttctattt atttaccagc 29281 gtgagtggat ctcagcaatt gatgagagcg atatatttat cctggttaaa tctcgacaga 29341 tcggcgctac tcatattatt gctttatggt ctttaattga tttgctcaaa acgggaaaaa 29401 ataaaatttt catgtctgca tcaaaggctc aggcatatca attcattgaa tatattaaag 29461 cctttgcact tgagatttgc ggcattgatt tgaccggaga tccaatcgtt attaatgggc 29521 cagtaaaaca ggccacagtt tattatttgg gtacaaatgc tttaactgca cagggacgac 29581 atggtgatgt catcatggat gaattcttct ggattcgtaa gttcttgcag tttaagaaag 29641 tagccagcgc gatggcttca caaaaaatgt ataaagaaat ttatatgtct acgccatcca 29701 gtatcttaca tgaagcttat aaattctgga ccggaacaga tagtggcaga aaactaccag 29761 ttgaaattga tgtaagtaaa tcagcactaa aaatgcctgt gaaatgtgcc gatggtaaaa 29821 ctcgccagat tgttacactg gcagatgctg aggccaaggg ctgtgattta tttgatcgtg 29881 aaaagctact gaatcaatat ggtgatgaag agtacgggaa tctttatgac tgtgagttta 29941 ttgatgatgc cggttcatac tttccgttaa aaattattac accaaacatg gtcgattcat 30001 ggatggtctg gaaggacttt aagccaaatg aaacaccaca ttatatcggt gaagtctggc 30061 tgggttatga cccatcattc acgggtgata atgcagcact cgctgtaatt gcaccaccga 30121 gaactccact tgagccgtat cgtattcttg aggttaaaca gttcaaaggt ttaagagcag 30181 aagaacaggc cctctatatt aaaaaagtct gtaatcgcta taacgtttca tttattggta 30241 tagacaatac aggtaacggt attgcagtag cagagcatgt ggctaagttt tttccatcat 30301 taacacggtt gaactataac cctgatttaa agatccggat gggtttacgt gccaaggaac 30361 tatttcaaaa aagaagactg cactttgatg caggtcttac cacagtagct aaagcttttc 30421 tatctattaa aaaagcctta accagtggtg gtggcaacaa aacattgatt accacgcgct 30481 ctgctgaaaa tgggcatggt gatgttgctt gggcaatcat gaacggtcta gaaaaggctc 30541 caattgttga taccaccgac gtaacccaac aaggcacaca cagtagccgt atccgagtat 30601 ataaatcatg aacctaaaaa atattctgtc tggatcttta cagaggttca cctccagtcc 30661 agcgaaagct atagcagagt cagcacaaaa aacgtcaata gtctgccgta attttggcga 30721 gcctgaaccg gttttggatg gccatacctt atttgaatat ggttattgtc cgaagtggct 30781 agactggtat gacttaccat acgatatgtt ggctacggct aaacttttca gagccaccag 30841 tcatcatacc agttcactta ttgttaaacg taatatcttg accagtgatt ttattccaca 30901 tccttggctt agtcggcatg attttaattc actcgcactg aatcttttaa cttttgcaaa 30961 ctgttatgta catattaaaa gaaatgcttt cggtggtatt gttggtttag gatctcggcc 31021 tgctatcaat atgcgtcgtg gtctagactt ggcaagtttt ttccaattag gctatgacca 31081 gactgacaat tatagatttg agcatagaga tattattcat ctatatgagt cggatatcag 31141 tcaagaggtg tatggagtac cgaactatct gagcagcatt aatgcaattc ttttgaatga 31201 atcagctacg ctttttcgtc gtcgttatta taaaaatgga gcgcatgctg gttttatctt 31261 acacatgact gatgctctgc aacgtcaaga agatattgat gctctggaag atgctttgga 31321 aaattcaaag ggcgctggta atttcaaaaa tttattactg tacacaccag gtggaaaaga 31381 aaaaggtgtg aatgtaatac ctctcgctga agtcgctgct aaagatgaat tttataatat 31441 caagattgct agtcgggatg atcagcttgc tggccatcgt attccaccac agttgctcgg 31501 tgtggtacca caaaatgctg gtgggtttgg tgatgttgag aaggccgcga aagtatttta 31561 tttcaatgag attatttact atcaaaactt attaaaacag attaatgaaa ggctgggagt 31621 agaagtaatt aactttaaaa aatatgagct tttatctaca gataaagagt aaataggtat 31681 caatatttta ttataaattt aataaaaaat atgtacttat aatgttttat tctccatgag 31741 aactaaagtg aagctggcat aaattgtcag ctttttttat gccaaaagtt ttttatatca 31801 tggaaagtat ctatcccata actaggttcg gctctgctta ttaagcgtat ggctacatca 31861 aataaatatc aaacagaata atcagctttt gaaaaagtta tcgaaaccct gatggccaaa 31921 cgcaatctcc tctcccgcct gcgcgcttac taaatatctc ttttttgatg cagttcaaaa 31981 aaatagctca gccccttatt tttaaggctt ttgaagcaga tttgataaaa gtttctgatg 32041 cactttgatg cggtctgatg catttaatac tagatttaaa taattttaat tttacttatt 32101 gcctattcta agaggttaca aaggttactg tatgaagaat atagatttaa gttgctgatt 32161 ataaataata tatgttgtaa cttttaaagg gttacattag gttacgcatc ttaataattt 32221 ttgattattt ccattaaaaa cataatctta ggaagttaca aaagtaactc tcaaaatagg 32281 ttacaagtaa ctctaaggta actcaaaagt aacttttaac tttataagaa ttgtatttat 32341 aaaacatata tttatatacc ataaactcta ttagtaactt ttgtaacctg ttttttttat 32401 gtcctaaaat tttaaaacct atgaaactta caattgaaaa gatatgccct aagttggccg 32461 taaacttgat gatatatata ggaaaaaaat ggaagataac tactctaata attcaaaagg 32521 agtcctataa ataattggac ttactgattg ttgcaccgta attgcaccgc gattatattt 32581 tttatattaa ctttttgatt attattaatt atttttaa //