LOCUS MF417954 16869 bp DNA linear ENV 27-FEB-2018 DEFINITION Uncultured Caudovirales phage clone 8AX_7, partial genome. ACCESSION MF417954 VERSION MF417954.1 KEYWORDS ENV. SOURCE uncultured Caudovirales phage ORGANISM uncultured Caudovirales phage Viruses; dsDNA viruses, no RNA stage; Caudovirales; environmental samples. REFERENCE 1 (bases 1 to 16869) AUTHORS van Zyl,L.J., Abrahams,Y., Stander,E.A., Kirby,B.M., Clavaud,C., Farcet,C., Breton,L. and Trindade,M.I. TITLE Novel phages from South African skin metaviromes JOURNAL Unpublished REFERENCE 2 (bases 1 to 16869) AUTHORS van Zyl,L.J., Abrahams,Y., Stander,E.A., Kirby,B.M., Clavaud,C., Farcet,C., Breton,L. and Trindade,M.I. TITLE Direct Submission JOURNAL Submitted (29-JUN-2017) Biotechnology, University of the Western Cape, IMBM, C2, Life Science Building, Robert Sobukwe Rd, Cape Town, Western Cape 7550, South Africa FEATURES Location/Qualifiers source 1..16869 /organism="uncultured Caudovirales phage" /mol_type="genomic DNA" /isolation_source="healthy skin from underarm of human male" /db_xref="taxon:2100421" /clone="8AX_7" /environmental_sample gene complement(<1..66) /locus_tag="8AX7_1" CDS complement(<1..66) /locus_tag="8AX7_1" /note="Predicted by MGA" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72441.1" /translation="MEQEQKEVIQNIYTTLGTTAGD" gene complement(70..213) /locus_tag="8AX7_2" CDS complement(70..213) /locus_tag="8AX7_2" /note="Predicted by MGA" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72442.1" /translation="MVKFTLQLLLISLMTLLTSGFISFHVGLAIYLLGSTIALLNYEK VEA" gene complement(206..526) /locus_tag="8AX7_3" CDS complement(206..526) /locus_tag="8AX7_3" /note="Similar to PF12728.2 [HTH_17] (hmmscan score : 45.6)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72443.1" /translation="MFNINIDEQEARELLEQAINQRVDELAREKFFMTYKELAEYLNL SKPTIEELLINNGMKYYMVGSTYRFKKSDVDAFMNQLTSHMDIHNNDLKQINVKKLME VLNG" gene complement(540..1181) /locus_tag="8AX7_4" CDS complement(540..1181) /locus_tag="8AX7_4" /note="Similar to YP_003344912.1 [Xylella phage Xfas53] (BLASTp score : 78.6). Similar to PF02498.12 [Bro-N] (hmmscan score : 70.4)." /codon_start=1 /transl_table=11 /product="putative Bro-N family protein" /protein_id="ASN72444.1" /translation="MIKQIFNDKEIRFIEKNDEYWSVAGDVAKVLGFRDANTAVRYLP THTRGTLKGRTTSDKKKARKFQDYTVINEKGIYRLVMRSNKTEAEEFQDWICDVLVEL RQSTGLKRYEAFRMLDKEKQKEAMDNLKNGIEVISKKDYCKAQAISNKAVSNVFGFPK MIKKQDMTQEMLELRQQVLNDTVEFMVFVDKYNLPLSVKKHIYDKYNDKQKMA" gene complement(1185..1403) /locus_tag="8AX7_5" CDS complement(1185..1403) /locus_tag="8AX7_5" /note="Similar to PF12844.2 [HTH_19] (hmmscan score : 42.6)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72445.1" /translation="MSVLINNESFKKAMFLKGYNLSDLSKELGIGISYLSSIANGKKI PSPKLAKSIANTLDVEIKDLFYFEEQEA" gene 1579..2238 /locus_tag="8AX7_6" CDS 1579..2238 /locus_tag="8AX7_6" /note="Similar to NP_839899.1 [Lactococcus phage P335 sensu lato] (BLASTp score : 59.3). Similar to PF13443.1 [HTH_26] (hmmscan score : 51.4)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72446.1" /translation="MIRNRLSELLSERGLKTSRVAKDVKIARSSLTSMIQNDSEMVRY DAIDKLCRYLNINVNDFFEYVPMNIELTIENIDNLTTFNNVKVLQNLSESMVSLYITA DFLIDIEFNNRDYDFDCELSLSEVKYNGFFNEFIFVLKNEDEHSNLKEKVDNLTPGFK KILFKKINNLLAENFKVDFLNKADEQISFPEFSEREKYEIQEAIKNSKFAIKSNIFTE Y" gene 2251..3426 /locus_tag="8AX7_7" CDS 2251..3426 /locus_tag="8AX7_7" /note="Similar to YP_008240364.1 [Thermus phage phi OH2] (BLASTp score : 292). Similar to PF00589.17 [Phage_integrase] (hmmscan score : 108.8)." /codon_start=1 /transl_table=11 /product="putative integrase" /protein_id="ASN72447.1" /translation="MASYDQISKNNWRYRISLGKNAETGKYEYISKTGFKRKSDAKHQ AEMIERQLRNGDYIAPSSSTFKQVAEDWIKQYANDVKVSSVRAREKAIQHAIKHFNTK PIQNIKKHDYQRFVDDMSTQYSKNYVDSIVASTNMIFKYAYDMKLIRVLPFEGIKRPK KKVSVEELEDSEIHKKFLEKDELFQFLEVAKNHHSPQNSFEVFTTLAYTGMRAGELLA LKWSDIDFENNIISITKTYYNPNNNKKHYQILTPKTESSIGKISVDPHVIQLLKDYKV NVQDTWKNELYVDNNFVFTDVSGYPLVIKKLSTWIQAIMKKTDITNKHISTHSFRHTH CALLIEAGVHIKEIQERLRHKDINTTMNIYAKITNSYKKDASQKFSQLMENVSKALF" gene complement(3629..3835) /locus_tag="8AX7_8" CDS complement(3629..3835) /locus_tag="8AX7_8" /note="Predicted by MGA" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72448.1" /translation="MRIKKDKYIDTEEVEKVKYSYPYFKRVSNIKIDNIDELEIYDET NHVFVGKSTVVIKGSDIFFLEFVS" gene 4200..4604 /locus_tag="8AX7_9" CDS 4200..4604 /locus_tag="8AX7_9" /note="Similar to PF07070.6 [Spo0M] (hmmscan score : 48.2)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72449.1" /translation="MFEKILTSIGIESLKINTVLKTEQVHSNGILNGSVVIESGASEQ TINQIELTLIERYDNPDERSQFPILDNELQTFTLHVDRSICENTKEIEHFQFNIHELE FKTNPDKLILKTRAYLSHSVDAYDEDEIRLID" gene 4802..5842 /locus_tag="8AX7_10" CDS 4802..5842 /locus_tag="8AX7_10" /note="Similar to PF07751.6 [Abi_2] (hmmscan score : 93.2)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72450.1" /translation="MKDINAIKTYLKTSKESKPYLSCKEQVILLKDRGLIIDNEDFAL LQLETISYYSLINAYSHFFKNKDNDQFVENTHFRDLFHCYQNDMRLKNLYFKYLILIE QSLKNNVAAVVAKNFGVNEPKTKSKNNPKLFDRKYSYLDTYYYDPHKSKRAGVLDDIS KSLNWNKVESINHYKKKHNHVPPWIIILPHNFGLTIKWLSILKKNHKIEVLKSVSNIT DNEKELSSIGVDSLNLLREFRNRIAHGHRFYSFKSESQLSRTHFNNIIGYELILKEDY AKDIGRNDLYALTLIILLFTRNKPTRDNLIEELFKLYGEMDQYNKINLLESIGFTYDL IRKLSFFNENYL" gene complement(7208..7345) /locus_tag="8AX7_11" CDS complement(7208..7345) /locus_tag="8AX7_11" /note="Predicted by MGA" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72451.1" /translation="MTVATPVFIDSIGTNEKENKKELERLSKEHPNAVFHIDDIGGFE E" gene complement(8473..8799) /locus_tag="8AX7_12" CDS complement(8473..8799) /locus_tag="8AX7_12" /note="putative ORF014; Similar to YP_239441.1 [Staphylococcus phage PT1028] (BLASTp score : 55.1). Similar to PB007737 [Pfam-B_7737] (hmmscan score : 67.2)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72452.1" /translation="MIYDKKSVRAFIMNYEPPKEEPPMYEENINNFFSLDQPTTSIET SDNFEEHIFFNELSSVIESIGTDKEFVVFDLLAHGWSYEKIGEMLMVTSGRVRQIFSR LLDKLP" gene complement(8796..9113) /locus_tag="8AX7_13" CDS complement(8796..9113) /locus_tag="8AX7_13" /note="Similar to PB000208 [Pfam-B_208] (hmmscan score : 35.1)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72453.1" /translation="MKTLKSFDYKILSGYIENYQTLVDEYKTQASQMTEQRYNRVKSI VKGITEVYNNATLQEQQLIKMLWWDKQPYDIIADVLGITEYTIKHAREVILRRVAERS TYL" gene complement(9110..9643) /locus_tag="8AX7_14" CDS complement(9110..9643) /locus_tag="8AX7_14" /note="putative ORF010; Similar to YP_239440.1 [Staphylococcus phage PT1028] (BLASTp score : 128). Similar to PB003455 [Pfam-B_3455] (hmmscan score : 185.0)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72454.1" /translation="MLKKNNNTFSYEREERISEYELLVKYNPQFINRKCQALEEQINA MYHLNISHMTCDEATGVVFTSYPLEKLVIWIIEKKEKLERYKNQSLERMNLLKNIVST YPYHEQQEIMHYMRTNGVYKPYKSIEKLCEDLYRNTHKARLMRQRDHLKEQRKYFDEE VEKVRTTLQTQREELVI" gene complement(9704..10177) /locus_tag="8AX7_15" CDS complement(9704..10177) /locus_tag="8AX7_15" /note="Similar to YP_002939708.1 [Staphylococcus phage phiPVL-CN125] (BLASTp score : 101). Similar to PF05119.7 [Terminase_4] (hmmscan score : 83.9)." /codon_start=1 /transl_table=11 /product="putative terminase small subunit" /protein_id="ASN72455.1" /translation="MPPRKLLSQQKGNLTVEQQENKKATEEAMEQLTPLNAEPPEWLD NRAKQEWFRIYPLLKELPIASLDLALVSAYCQAYSDYIQATERMKQEGAVVITERGTK LNQNHAIKRDALAQLNSISSKLGLTVESRLKILDPKNEIPKKQSVYDQFGISDND" gene complement(11585..12043) /locus_tag="8AX7_16" CDS complement(11585..12043) /locus_tag="8AX7_16" /note="Predicted by MGA" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72456.1" /translation="MITAIRYKGNRAKVTGNGEDLMILASSSNVPVELADVDNKLNIV SDFEEGEIEPKAVKKIKFNKNSCVMMNGNLNFIIASSQRNNREFIVKKNHTLIHAWKS GDHAKDKAFEQIKNNKVDSVDVYVKNAEGKDNRVSHIGEILAVSINLKSA" gene complement(12056..12676) /locus_tag="8AX7_17" CDS complement(12056..12676) /locus_tag="8AX7_17" /note="Predicted by MGA" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72457.1" /translation="MSKTVKENSISIFDEQIYGKRLRAKEVQKQYDQLVDRIKKNNAK IIHYQHQDEFAEATKLKRQQADLEQELLEIDEQLKTSNYSITDDEFTSFYDAYNSEMQ DIKKAHEQYRKEMKDKLQEVAATYRKMIENKNEGGRRISRLRYVKQEQQHPSNIHNQY KGQMLADEIKIGGNTTPRDYAWLLEDMLKEESLEDFQKYHFGKEKW" gene complement(12888..13199) /locus_tag="8AX7_18" CDS complement(12888..13199) /locus_tag="8AX7_18" /note="Similar to NP_795451.1 [Streptococcus pyogenes phage 315.2] (BLASTp score : 83.2)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72458.1" /translation="MIEINKPEHFEELNEVKKNALLEFCYSMNKIKTFNTRRSSYGLK HVFERKYRKVLSGTFESSYVTNGQFKGAMLKAGFDVKDKSQLNWHFNVSEKSIKELDE R" gene complement(13489..15759) /locus_tag="8AX7_19" CDS complement(13489..15759) /locus_tag="8AX7_19" /note="Similar to NP_690795.1 [Bacillus phage phi105] (BLASTp score : 164)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72459.1" /translation="MAIKEKDKIIEVNALEIPEELKELPQWVLWRAEWDEKQQQFRKV PYNFSGYRASSTNKDTWTIFDVINRLNEDNENYNGIGFMLSDDDNYIVLDIDNAIDEH GQIISDLTLDMTEITYCEKSPSGTGLHCFFKGQLPSERKKKRSDLDIELYDNARFMTV TGESIGQSEICDEQDILNNLVDRYFKQDKPPEKNTAQISNVSETLSDDEVIDLMLKSK QKDKISDLLKGNYEPYFDSPSEAVQSLLHYLAFYTGKNKAQMERIFLNYNNLTDKWDS KRSNSTWGELELDKAINNQSEVYKNPHLNITVCGTTNNLIKKDSWWIVPEEGSNKKLK FAHTVMAEYILQEYHIVRYPDSDGDVYIYNPESGIYKIDKTGRKLRQIIRKLETLKDN QVKEVRNYIVDTCRVESEICQDYVVAKNGLIHFKTKAFRPFTPEIFVINKIPTAYNPN AYDEFVDNTIKKVSCNHEPTKKNIYEMFSQVLYPNLLIDKMFYLLGTVADNGKSTVQH MIKATFDSGGQISSVSPQRLANNNFAGSSMYGKMANIVDDLPNVEIVDTGNIKTTVTG GYLEIEEKGKGSHSVRMNTPFIVASNHYPKFKESGEQINKRLHIIPFEYSFKDDENRL SVSESTNKIYSESAKEYVLKLAIDTLSDMLERKGSYITPNERSDNSIEIFEDNNNPLS EYLEFRDLDYFLTTPWTTIYTDYRVFCNNNYIRNPIDKSDFKTIIENKFNIVWKGSVK FTNGEKSFVKPGFRYK" gene complement(15861..16160) /locus_tag="8AX7_20" CDS complement(15861..16160) /locus_tag="8AX7_20" /note="putative ORF016; Similar to YP_239451.1 [Staphylococcus phage PT1028] (BLASTp score : 81.3). Similar to PF07342.6 [DUF1474] (hmmscan score : 141.9)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72460.1" /translation="MDWELRNLFSDLQIVQEKINDVVTSFVWFDDKYFTHEPSHVLTE KEVNTHGLKYHEHRIKNTHVIDLMLMYMEDFDDIMKKIHEIEKASSCTDQSQDNA" gene complement(16175..16381) /locus_tag="8AX7_21" CDS complement(16175..16381) /locus_tag="8AX7_21" /note="Predicted by MGA" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72461.1" /translation="MAAKLDVNKQNIMRAINWIIKNEEEIIFESQSQLSFFSREDLEK IDYCKRTLESLIEAKEIYNKQKIS" gene complement(16392..16730) /locus_tag="8AX7_22" CDS complement(16392..16730) /locus_tag="8AX7_22" /note="Predicted by MGA" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72462.1" /translation="MITEILKAYDDMAIPAMNVSQLRGETERLSELIGYLTEKAKAYR EEKDIKGAEAIEQIVLDDLQFEFESVYGQFEEEFKNWEQKYKKLEKVCKYYGIPVPSL KSEKVINFYK" ORIGIN 1 atccccagca gttgttccta acgttgtata aatattttga ataacctctt tttgttcttg 61 ttccatcttc tacgcctcca ctttttcata atttaataat gcgattgtgc tgcctaataa 121 ataaattgct aaacctacat gaaaagatat aaatccacta gttaataaag tcattaaact 181 gattaataat agttgtaatg tgaatttaac catttagaac ctccatcaac ttcttaacat 241 taatctgttt taaatcattg ttgtgaatat ccatgtgaga tgtgagttga ttcataaacg 301 catcaacatc tgatttctta aatcggtatg tgctgcctac catgtaatac ttcataccgt 361 tattaataag tagttcctca atcgttggtt tacttaaatt taggtattca gctaattctt 421 tgtatgtcat aaagaacttt tccctagcca gttcatcgac acgttgatta attgcttgct 481 ctaataactc acgtgcttct tgttcatcaa tattgatatt gaacatcggt tatacctcct 541 tatgccattt tttgcttgtc attgtatttg tcgtaaatgt gctttttcac agataaagga 601 agattgtatt tatcaacaaa taccataaat tctactgtgt catttaacac ctgctgtctt 661 aactccagca tttcctgtgt catatcttgc tttttaatca tcttaggaaa gccgaacaca 721 tttgatactg ctttattact aattgcttga gctttgcaat agtctttttt agaaataact 781 tcaataccat tttttaaatt atccatagct tctttttgtt tttctttgtc taacatgcga 841 aaggcttcgt atcttttaag ccctgttgat tggcgtaatt caaccagcac gtcacaaatc 901 caatcctgga attcttcggc ttcagttttg ttagaacgca ttacaagtct ataaatacct 961 ttttcgttga taactgtgta atcttgaaac tttctagctt tctttttatc tgatgtggta 1021 cgccctttta gagtacctct tgtatgcgtt ggtaaatatc taacggcagt atttgcgtct 1081 ctaaaaccca atacctttgc tacatcacca gctactgacc aatactcatc gtttttttca 1141 ataaaacgaa tttctttatc attgaaaatt tgtttaatca ttggttatgc ctcctgttct 1201 tcaaagtaaa ataaatcttt aatttcaaca tctaaagtat tagcaatgct ttttgctagt 1261 ttaggactag ggatcttttt accatttgca atagaactta aataagatat tccgattcct 1321 agttctttag ataaatctga taaattatat ccttttaaaa acattgcctt tttaaaagat 1381 tcattattaa ttaagacaga catttcatgc aactccttcc ttttttgatt gacttctcaa 1441 tcaatttata actacattat acataattaa aaaaatattg caataagttt ttgattgact 1501 tctcatacat tttaaataaa tttatacacg ttagattgat ttaatgataa tataggtaat 1561 ggaaaaggag ggcaaaaaat gattagaaat agattatcgg aattactttc agaaagaggt 1621 ttaaaaacat cacgtgtcgc taaagatgta aaaattgcta gaagttcact tacctcaatg 1681 attcaaaatg attcagaaat ggtaagatat gacgctattg ataaattatg tagatactta 1741 aacattaatg ttaatgattt ctttgaatat gtgcctatga atatcgagtt aactattgaa 1801 aatatagata atttaactac ttttaataac gttaaagtgc ttcaaaattt gtcagaatca 1861 atggtttctt tatatataac tgctgatttc ttaattgata tagaatttaa taatagagat 1921 tatgattttg attgtgaatt atctttaagt gaagtgaaat ataatggttt ctttaacgag 1981 ttcatttttg ttctaaaaaa tgaagatgaa catagcaatt taaaagaaaa ggttgataat 2041 cttactccag gttttaaaaa aatattattt aaaaaaataa ataatttact agccgaaaat 2101 tttaaggttg attttttgaa taaagctgat gaacaaattt cttttcctga attttctgaa 2161 cgtgaaaaat atgaaataca agaagcaata aaaaatagta agtttgctat taaatctaat 2221 atttttactg aatattaagt aggtgttcct atggcaagtt acgatcaaat atctaaaaac 2281 aactggcgtt atcgcatatc actaggaaaa aatgcagaaa caggaaaata tgaatatatc 2341 tctaagactg gctttaaacg taaatcagac gctaaacatc aagctgagat gatagagcgt 2401 caattaagaa atggagacta tatcgcccct tcttccagca cattcaaaca agtagctgaa 2461 gattggatta aacaatatgc taacgatgta aaagtaagta gtgtaagagc gcgtgagaaa 2521 gccatacagc acgccataaa gcactttaat actaaaccaa tacaaaatat caagaaacat 2581 gattatcagc gctttgtgga cgatatgagc acacagtata gtaagaatta tgttgatagt 2641 attgtggcat ctactaatat gatatttaaa tatgcttatg atatgaaatt gattagggta 2701 ctaccttttg aaggtattaa acgacctaaa aagaaagtaa gtgtagaaga attagaggat 2761 agtgagatac ataaaaaatt tcttgaaaaa gatgaattat ttcaattcct ggaggttgct 2821 aaaaatcatc attcgccaca gaatagcttt gaggtattta ccacattggc atacactggc 2881 atgcgtgcag gcgagttgtt agcattaaaa tggtctgata tagactttga gaataacata 2941 attagtatta ctaaaactta ttacaatccg aataataata aaaagcatta tcagatactt 3001 actccgaaaa ctgaaagctc aatcggtaaa atctcagtag atcctcatgt gatacaatta 3061 ctcaaagatt ataaggtaaa tgtccaggac acttggaaaa atgaattata tgtagataat 3121 aattttgtgt ttactgatgt gagtggttat ccacttgtga ttaaaaaact gtctacatgg 3181 attcaagcaa ttatgaaaaa gactgatatt actaataaac atataagtac acactcattc 3241 cgtcatacac attgtgcgtt actgattgaa gcaggtgtac atatcaaaga aatacaagaa 3301 cgcttacgcc acaaagatat aaataccaca atgaacatct acgctaagat taccaactca 3361 tacaaaaaag acgcttccca aaagtttagt caactcatgg aaaacgtcag taaagcatta 3421 ttttagaatt tttatgtcat tattatgtca tgcggaataa cctaacgcct ttaaaccaac 3481 gtttattggc gtttttacat catacctggc attatgtata actaaaaata agatataata 3541 catgtttaaa atcttattaa atcagtactt tcatatttta attataccat tattttcagt 3601 ttttctattc tattatgtca taattatgtc atgacacaaa ctctaagaaa aagatgtcgc 3661 tacccttaat taccacagtc gatttaccaa caaaaacatg atttgtttca tcatagattt 3721 ccaactcatc tatattatct attttaatat ttgatactcg tttaaaatac ggataagaat 3781 atttaacttt ttctacttct tcagtatcaa tatatttgtc tttttttata cgcacacgcc 3841 aataaaagat tgatatgtat attttgtctt ggattaactg atttatattt gatgttagtc 3901 aaaaatcttc atcattttta atttttaaat attttttctg tatttgaata tttcagttat 3961 atacattcac tatactaaac attttgtgat atagttatcc gtcttttcgt ttctcgattc 4021 aacacagagc ctctctcagc gtcttaaaat aattaataac ttaatatttt atatacgtta 4081 tacattttaa attagtaaaa tgataaaatt atttatatat tcgttatggc aaatatgtta 4141 ttcagtttga gccattacga gtgtttgaat ataaagatat agaataagga ggaattgaaa 4201 tgttcgaaaa aatattaact tcaataggta tagaatcatt aaaaataaac actgtgttaa 4261 aaacagaaca agttcatagt aatggtatat tgaacggaag cgtggtcata gaaagcggtg 4321 catcagaaca aactattaat cagattgagc ttacattaat tgaaaggtat gataatcccg 4381 atgagcgaag tcaatttcct attttagata acgaattaca aacatttact ttacacgtag 4441 accgtagtat atgtgaaaat accaaagaaa ttgaacattt ccaatttaat attcatgaac 4501 tagaatttaa aactaatcct gataaattga ttttaaaaac acgtgcttac ctttctcatt 4561 ctgttgatgc ttacgatgaa gatgaaataa ggctgattga ttaattgtgt tctatttttt 4621 caatataaat aaaggaggtt gagagatgga agaagcttgt tagtttcaag agacatcttt 4681 aatattgact aataaagaaa aaacatctat aatgcaagta tgaaatagtt gttctatgaa 4741 acgactcaag aaacctctca tgcttgcatg aggggttttt tcgttgtagg agagaattct 4801 attgaaagac ataaatgcca taaaaactta tttaaagact tccaaagaat caaaaccata 4861 tttaagctgt aaagaacaag taattttatt aaaagataga ggattaatta ttgataacga 4921 agattttgcg ttgctacagc tagaaacaat ttcctattat tcattgatca acgcttattc 4981 tcactttttt aagaataaag acaacgacca atttgtagaa aatacacatt tcagagattt 5041 gtttcattgt tatcaaaatg atatgagatt aaagaattta tattttaaat acttgatatt 5101 gattgaacaa tctttaaaga ataatgttgc tgcagtagta gccaaaaact ttggagtaaa 5161 tgaacctaaa acaaaaagta aaaataatcc gaaacttttc gatagaaaat acagttatct 5221 agatacttac tattatgacc cccataaaag taaaagagct ggagtattag atgacattag 5281 taaatcttta aattggaata aagtggagtc aattaatcat tataagaaga aacataacca 5341 tgtaccacca tggataatca tattacctca caatttcggt ttaacaatta aatggttaag 5401 tattctaaag aaaaatcata aaattgaagt tttaaaaagt gtttctaata taacagataa 5461 tgaaaaagag ttatcaagta taggggtgga ttcactgaat ttattaagag aattcagaaa 5521 cagaatagca cacggtcata gattttattc ttttaaatct gaatcccaat tatctcgaac 5581 gcatttcaat aatataattg ggtacgaatt aattttgaaa gaagattatg ctaaagacat 5641 tggaagaaat gatttatatg cattaacgtt aataattctt ttgtttacta gaaataaacc 5701 aacacgagat aatttaatag aagaattatt taaattatac ggagaaatgg atcaatacaa 5761 caaaataaat ttattagaat caatcggttt tacttacgat ctaatacgaa aactttcttt 5821 ttttaatgaa aattatcttt gaaagtttaa tcaataattt tgggtacacc accgtaccct 5881 tattattttt tgctttttaa ttgttaaaaa acgccacttt gtaagtgacg tttaagatga 5941 gatatttaac ttgtctgtgt ggtaattcat acttttgcct tttgtttcat aaatatgccc 6001 tttttatgcc cttaaattta agattacaac agttttatct taaaagtgca actttcaaat 6061 cactcattct acttcaaaca cttgatacac cttacttcat atacttttta aaactcctga 6121 aatgttgcat gaattgatgt ttattatgat tattattaga cgtatatcaa tgctttaaac 6181 actatagtta cacaaattaa tatctcactg taacacagag cgtttctcag cataaaaaaa 6241 cacatcaata aagatgtggc tacatataaa aatatcagtt tgcccttttg tatctattta 6301 aaaattaaac tgataaacca aaaaaatagg attagcgaat tagtatggta ttgcttcttt 6361 ctgcactcac catcaaaaat tataaaatag gtctctatgc cgattctctt atcactttaa 6421 aatgataagc ttataaaaat aaataaaagg caggacattt atgaatattt taaaatcaaa 6481 ttatttacta ttgctttctc tattaatcaa tatattattt ttctttatcc ttttcatatc 6541 aattattttc aaaatcactt ttattcatgt aattgataat ctttcgttcc attggtttta 6601 tactacattt ggcgaaccaa aaatgaatta ttctaatgga ttgtttaacg attatatgac 6661 tttgtcagct acttacggag ataaaagcac ttttataatt attactatta ttatttcaac 6721 agtcgctttt atcaagcgaa attttatatt ttctttatgg aatatattaa cagtggccat 6781 aggtggcatt gttggtatag gcttaaaata tcttcttcat agatcaagac catttgacca 6841 tttgattaca gatactggct attctttccc aagtgggcac tcactagcaa gcacactagt 6901 tatatttata cttatttttt tactaataaa tcatttaaaa aataaaacgt taacaataat 6961 aatatctagc gttttgatat ttatttggat aagcattcta ttttcaagat tatattttca 7021 cgcacatttt ttatccgacg tattaggtgg ggtaactttc agtttagctt ggttaaattt 7081 atcatatttt ttgtttagaa aacatctata ctaatctaat tttaaattaa ctcatactaa 7141 aaaagctacc aatcacggta gcttttctat attagaaata taaaattacg tagatttatt 7201 gcttaattta ctcctcaaat ccacctatat catcaatgtg aaatactgcg ttagggtgct 7261 ctttacttaa tctctctaat tcttttttat tttctttctc atttgttcca atactatcaa 7321 taaatacagg tgtagctaca gtcacttacc taacgataga tgaggtaacg ttttgttccc 7381 tcatctatgg gggagcgttt cgcgacccca tgaataaagg gcatcgtgtt tccctactcc 7441 ctttgctatc tgactgaatg aattgtgcat ggagctcccg tcacgaattt tacagcagta 7501 tataataaaa tatatgtcta ttaagtgaag tagagatatg ttaaaataat tcgtagttag 7561 cattgctttc attgaaactt agtcttacta gtcatgtact agtgagactt tttcgcatac 7621 aaaaaggggc tttacgcccc taaagagtta tgcgatttat gaaagctttt aaagtgtcga 7681 gattgcctaa acatacagta ttttcgatgg agaagaaata cttaatggaa aggaaagagt 7741 cgccagttag acaatctcaa ttattattat tacatactaa tttttattta gccacactat 7801 tataataaaa gtcattgatt tgtatgtaat agagtaataa atacgtttca ctccatactt 7861 tttatctgca ctctcactta gtccgtcata agctaagttg agagtgattt tttatacaaa 7921 aaaagaagct aaccaattaa ggttagctcc tatgtagtcg aggtagagta aaaaactcta 7981 tatgtaacat aaagacttta gaaatcatta aatctctttt attattatct cattatttat 8041 atatttgtct acaataaaat aaaagactgc atccaaagga tacagtcagt ttattaaagg 8101 atactcaatt ttgagtagta agtttagtag ctgcatttat tatcttataa aaggctctaa 8161 atgtaaatag aaagcgttta ctttttataa ctaaaaaata atactactca aatatgtata 8221 tctctcttac ttctttttct taagagattt aaagactaga gctaaaatta agaatattaa 8281 agcaacagtg agaactgacc ccatttatat tccctcctag atatctttaa atttgatact 8341 aacaaactaa ttccttaatt aaaactaaat atacaaaata aagggtacta caaaatacca 8401 ataccttaat tatttattac ggtcactaac taattttatt tgaatatata tctacataga 8461 aaaagccacc tcttagggta gcttatctaa taatcggcta aatatctgtc gaacacgtcc 8521 actcgttacc atgagcattt cgcctatctt ctcatacgac caaccatgag cgagtaaatc 8581 gaaaacaacg aattctttat ctgtaccaat gctttcaatt actgacgata attcattaaa 8641 aaatatatgt tcttcaaaat tatcgctcgt ttctatcgat gttgtaggtt gatctaatga 8701 aaagaaatta tttatatttt cttcatacat gggcggttct tctttaggtg gttcatagtt 8761 cattatgaag gctctaacgc ttttcttatc gtatatcata gatatgtact tctttcagca 8821 acacgtcgta atattacttc tctagcatgc ttaattgtgt actctgttat ccctaagaca 8881 tctgcgatta tgtcataagg ttgtttatcc caccataaca ttttaattaa ttgctgctct 8941 tgtagtgtcg cattgttata tacttcggta atccctttta cgatactttt aactctattg 9001 tatctctgtt ctgtcatttg actcgcttgt gttttgtact catcaactaa tgtttgatag 9061 ttttctatat atccactcag tattttataa tcgaaactct ttaaggtttt catataacta 9121 gctcctctct ttgcgtttgt aatgtagttc ttactttctc tacttcttca tcaaaatatt 9181 ttctttgttc tttcaaatgg tctctttgac gcattagacg agctttatgc gtgtttctat 9241 ataaatcttc acataacttc tcaatgctct tataaggctt ataaacgcca tttgtacgca 9301 tatagtgcat tatctcttgt tgctcatgat atggataggt gctgacaata tttttaagta 9361 aattcatgcg ttccagtgat tgatttttat aacgttctaa cttttctttt ttctcaatga 9421 tccatataac taatttctct aatggataac tggtgaaaac tacccccgtt gcttcatcac 9481 aggtcatatg tgagatattt agatgataca tggcatttat ttgttcttcg agtgcttggc 9541 attttctgtt tataaattga ggattgtatt taaccaacaa ctcatattct gaaattcttt 9601 cttctctttc ataactgaaa gtattattat ttttcttcag caacagctct gcactccttt 9661 aaactttatc tattttattt cctgattgac tttatacttt tgtttagtcg ttatctgaaa 9721 ttccgaattg gtcatataca gattgtttct taggtatttc attcttaggg tctaatatct 9781 tcagtctaga ttctactgtt aagcctaatt tagatgaaat actattcaat tgtgccagtg 9841 catcgcgttt aatagcgtgg ttttggttta atttagtacc tctttcagta ataactactg 9901 cgccttcttg tttcatacgt tcagtggctt gaatataatc cgaataggct tggcaatatg 9961 cggacaccaa cgctaaatct aaactggcaa ttggtaactc ttttagtaat ggatatattc 10021 taaaccattc ttgttttgct ctattatcta accactctgg aggttctgcg tttaaaggag 10081 taagctgttc cattgcttct tccgttgctt tcttattttc ttgttgttcg actgttaaat 10141 tacctttttg ttgagataat aattttcttg gcggcatttt cgtcctcctt tctaagtcat 10201 ttaatgtaaa gtaaaaagcc tttacgaata tttaagattt catttagaaa tttattgaaa 10261 gttaagtgcg gctcgtttaa ctcgaatcct agagccacag ggggtttata tcgccccgtt 10321 aattttataa agaattattt cctgaaaatt aatttttgtg aattttatta tggcagctaa 10381 aacacactac ctctaaatta tccatatcca gtcttttaac ccagtccctc gccattatct 10441 atcgatatac atttatcttc actaattatc atgtccttta attgcttcat ataagcctgt 10501 cattagcttt taatcagttc tatatatatt tttaatccta tttgttctta tcttccttat 10561 aaaaagccta aaacagcatc ttaaactgtt ctatgttacg tttgttctct caacatttct 10621 atcatcattc ttcattatct tttatttata atttcttctt tcttgatcta aataactttt 10681 ctttatttca aatcaaaaac aaatctaaat cactaagcaa ttttaaatta ttttttattt 10741 tcatttctca tttcaaaatt cgatttcact ttctgaattt caaaatactt tctgacttct 10801 catttacttt tataattttc attctcttta tcattctaat atctttaact gcaactcaac 10861 tttgttttat tcaatcaatg aattatcttc ttaaatcttt tatcttctat cataaaaaga 10921 taattgtttt attctacttc atttcttcta aatgctttcg atgtttaaca caaacataat 10981 tgtttcttct acttcaatat aattaatatc tctaacacat attggattac ccatatatat 11041 tttagtgtag tgcctaaaca ctatatcaag ctattacctt aatactatgg aataatacct 11101 atgcccttta tactagctgc agcgtacact tttatacggt cacacaatta tagccctgta 11161 acttatgtga gggtactcat ctataacctc tgtttacttg tactgtatga tagttaatcc 11221 tactttgtct gcctatccta taactcacct acacgttgtt tagttgtata ctgttgattt 11281 atctatacta tgcgattaat atactctgcc acatattaat tacttcatat tgtttttggt 11341 ttacctatac ttcctattaa gtatactatt ggctcactta tacacgagac cacttataaa 11401 agaaaggcca tcacatatat taaaatgatg accttgtaaa catgccttag acatagaaat 11461 tcaattgaac aaatgaagga gaaactcata gctattttag actttaacaa agagcgctcg 11521 ttattaaaga tatttattat ccttaatatg tttattataa cataaaaggc tcaaccatgc 11581 tattttaagc gctttttaaa ttgatagaaa cagctaaaat ttcacctatg tgacttaccc 11641 tattatcttt accttctgca ttttttacat acacgtcaac actatccact ttattatttt 11701 tgatttgttc aaatgcttta tctttcgcat gatccccact cttccaagca tgaatgagtg 11761 tatgattttt cttaactata aactccctat tatttctctg acttgaagca ataataaagt 11821 tcaaattgcc attcatcatg acacaactat ttttattaaa cttaatcttt ttaactgctt 11881 tcggttcgat ttctccttct tcaaagtcac tcactatatt taacttatta tctacatcag 11941 ccagttctac aggtacatta cttgaactag ctaatatcat taaatcttca ccatttccag 12001 ttacctttgc tctgttccct ttgtatctaa tggctgtgat catcgcatta cctccttacc 12061 atttctcttt gccgaagtga tatttttgga agtcctccaa tgattcttct tttaacatat 12121 cttcaagcaa ccatgcgtaa tctctaggag ttgtattgcc cccgatttta atttcatcag 12181 caagcatttg ccctttgtat tgattgtgaa tattactagg gtgttgttgt tcttgtttga 12241 catagcgtaa tcgtgatata cgtctgccgc cttcattctt attctcaatc atttttcgat 12301 aagtggctgc cacttcttga agtttgtcct tcatctcttt tcgatactgt tcatgtgctt 12361 ttttgatgtc ttgcatttca ctattatatg catcgtagaa tgatgtaaat tcatcatctg 12421 taatactgta attcgatgtt ttaagttgtt catctatttc caataactct tgttctaaat 12481 cagcttgttg acgctttaat tttgtagctt cggcaaattc atcttgatgt tgatagtgta 12541 taattttagc attatttttt ttaatacgat ctactagctg atcatattgt ttttgaacct 12601 ctttagcacg taatctttta ccataaattt gttcatcaaa aatactgatt gaattttctt 12661 ttactgtttt actcatactt cgtaacctct ttttttcatt tttttaaata ttaatctttt 12721 aatcacatca gagtaatgtg tccaacactt attgtttgaa tttgtgttat atttccaaat 12781 tttatcacta ttatattgtt tacaataata attgttacct ggtactaatt atttacttaa 12841 attataccac aaaaggctct gttaagccaa ttttggtagt tttatagtta tctttcatct 12901 aattctttaa tgcttttttc actgacgtta aaatgccaat ttaattgact tttatctttt 12961 acatcaaatc cagcttttaa cattgcacct ttaaactgcc catttgtcac atagctactt 13021 tcaaaggtac cagacaatac cttacgatat tttctttcaa aaacgtgttt taagccataa 13081 gaagaacgtc tagtgttaaa agttttaatt ttattcattg agtagcagaa ttctaatagt 13141 gcatttttct taacttcatt taattcttca aaatgttctg gtttatttat ttcaatcatt 13201 tttatatctc cttttctatg aggtagcttt ttcaataaaa atactaccaa aaactaccaa 13261 ctagctaccc ggttgaagtc agtcacatca caggttttta ctaatttagt agtcggtagc 13321 cttaattttt tactcacagt gatatttttt tagttactat tacctatata aaatattttc 13381 taatgtttaa aaatagcact actcactacc taaatctttc aaccccttgg tgtgactgtt 13441 ctcaagcagg tagtggaaaa gtagttttag gtagttttag tagttttttt acttatatct 13501 aaaaccgggc ttcacaaaag atttctcacc atttgtgaat ttaacggaac ctttccatac 13561 aatattaaac ttattttcaa ttatcgtttt aaagtcagat ttatctatcg gatttctgat 13621 atagttgtta ttacaaaaaa ctctataatc ggtgtatatc gttgtccacg gtgtagttag 13681 aaaataatct aaatctctaa actcaaggta ttcacttaga ggattgttat tatcttcgaa 13741 tatctcaata ctattgtcag atcgttcatt aggtgtgata tatgagcctt tacgttctaa 13801 catatcgctt agtgtatcaa ttgctagctt aagcacgtac tctttagcgc tttcactata 13861 aattttattg gtgctttcag aaacacttag acggttctca tcatctttaa aactgtactc 13921 aaacggtata atatgcagac gcttattgat ttgttcgcca ctctctttaa atttaggata 13981 atgattactg gcaacaatga atggtgtgtt catacgcacc gaatggcttc ctttaccttt 14041 ttcttctatc tctaaataac cacctgtgac agtcgtttta atatttccag tgtccacaat 14101 ttcaacatta ggtaaatcat caacaatatt cgccattttt ccatacatac ttgaaccagc 14161 aaagttgtta ttggctaaac gttgtggact aactgatgaa atttgaccgc cactatcaaa 14221 agtagcttta atcatatgct gaacggtaga tttgccgtta tctgcaacag tccccaaaag 14281 ataaaacatt ttatctatca gtaaattagg gtataatact tgtgagaaca tttcataaat 14341 attcttttta gtaggctcat gattgcagga tactttttta atagtattat ccacaaattc 14401 atcataagcg tttggattat atgcagtagg gattttgttt attacaaaaa tttcaggtgt 14461 aaatggtctg aacgctttag ttttgaaatg aatcaaacca tttttagcca ctacataatc 14521 ttggcaaatc tcagattcaa ctctgcaagt atcaacgata tagtttctta cttccttaac 14581 ttgattgtct tttaaggttt caagctttct tataatttgt cgcagtttac gacctgtttt 14641 atcaatcttg tatataccac tctcaggatt gtaaatataa acatctccat ctgaatcagg 14701 gtatcttaca atatgatatt cttgaagaat atattcggcc ataactgtat gagcaaattt 14761 cagcttctta ttagaacctt cctctggaac tatccaccaa ctatcttttt taattaaatt 14821 gttagtagtt ccacacactg tgatgttaag atgtggattc ttatacactt cactctgatt 14881 atttatagcc ttatctaact ctaattctcc ccacgtacta ttactacgct tggaatccca 14941 cttgtccgtt aaattgttat agttcaagaa aatacgttcc atttgagctt tatttttacc 15001 agtataaaat gctaggtaat gcagtagact ttgcactgct tcgctaggac tatcaaaata 15061 cggctcgtaa ttgcctttta acaaatcact aattttatct ttttgtttag atttaagcat 15121 aaggtcgata acttcatcat ctgacagtgt ttcgcttaca ttagaaattt gagcagtatt 15181 tttttctggt ggtttatctt gcttaaaata gcgatcaact agattgttta aaatatcttg 15241 ctcatcacag atttcagatt gtcctattga ttcacctgtg actgtcataa atcttgcgtt 15301 gtcatatagc tctatatcta ggtcagaacg ctttttctta cgttctgatg gtaattgtcc 15361 tttgaagaaa caatgtaaac cagtaccact tggagacttc tcacagtagg tgatttcagt 15421 catgtccaat gttaaatctg aaatgatttg accgtgttca tcaatcgcat tatcaatatc 15481 taacacgata taattatcat catcacttaa cataaatccg attccattat aattttcatt 15541 atcttcatta agtctattga taacatcaaa tattgtccac gtatctttgt tggttgagct 15601 tgcacggtaa ccactaaagt tatacggtac ttttctaaat tgttgttgtt tttcgtccca 15661 ttcagcgcgc cataataccc actgtggcaa ttcttttaat tcttcgggta tttctagcgc 15721 attaacttca attattttat ctttttcttt gattgccatt aaattcctcc taaacacttc 15781 taaaattcag cacggaatgg taaaataaaa atgtgttaaa taagccattc catgcttgtt 15841 ttttatacta tttcaaatat ttatgcgtta tcttggcttt ggtcggtgca agatgacgct 15901 ttttctattt catggatttt tttcataatg tcgtcaaaat cttccatata cataagcatt 15961 aaatcaataa catgtgtgtt tttgatacga tgctcgtgat atttcagccc gtgtgtattt 16021 acttcctttt cagttaacac gtggctaggt tcatgtgtga aatacttatc atcaaaccag 16081 acaaaagatg ttacaacatc attaattttt tcttgtacta tttgcaaatc actaaataaa 16141 tttcttaatt cccaatccat tattacttaa ctccttaact aattttttgt ttattataaa 16201 tttctttagc ttcaattaaa ctttctaaag tacgcttaca gtagtctatt ttctccaaat 16261 cttcacgact gaagaaactt aactgacttt gactttcaaa tataatttct tcttcatttt 16321 taataatcca gttgatagca cgcatgatat tttgtttatt cacatctaat ttagcagcca 16381 ttgtttcact cctatttgta gaaatttatt actttttcac tttttaatga aggtactgga 16441 ataccgtaat atttacatac tttttcaagt tttttatatt tttgttccca attcttaaat 16501 tcttcttcaa attgtccata cacactttca aattcaaatt gaagatcgtc taatacaatt 16561 tgctcaatag cttcagcacc tttaatatct ttttcttctc tgtacgcttt tgccttttcc 16621 gttaaataac ctattaattc tgaaagtctt tctgtttcac ctcttaactg agatacattc 16681 atagctggta ttgccatatc gtcatatgct tttaaaattt ctgttatcat tttatttcac 16741 tccatcaaaa ttattttcaa tttgttgtat tgcccactca atgattgctt gtaagtgttc 16801 ttcacgatta actgtttcag tccattcatt atgaccttct ttaaaatgat gttcatattc 16861 tgttgcttt //