LOCUS MF417960 15565 bp DNA linear ENV 27-FEB-2018 DEFINITION Uncultured Caudovirales phage clone 9F_4, partial genome. ACCESSION MF417960 VERSION MF417960.1 KEYWORDS ENV. SOURCE uncultured Caudovirales phage ORGANISM uncultured Caudovirales phage Viruses; dsDNA viruses, no RNA stage; Caudovirales; environmental samples. REFERENCE 1 (bases 1 to 15565) AUTHORS van Zyl,L.J., Abrahams,Y., Stander,E.A., Kirby,B.M., Clavaud,C., Farcet,C., Breton,L. and Trindade,M.I. TITLE Novel phages from South African skin metaviromes JOURNAL Unpublished REFERENCE 2 (bases 1 to 15565) AUTHORS van Zyl,L.J., Abrahams,Y., Stander,E.A., Kirby,B.M., Clavaud,C., Farcet,C., Breton,L. and Trindade,M.I. TITLE Direct Submission JOURNAL Submitted (29-JUN-2017) Biotechnology, University of the Western Cape, IMBM, C2, Life Science Building, Robert Sobukwe Rd, Cape Town, Western Cape 7550, South Africa FEATURES Location/Qualifiers source 1..15565 /organism="uncultured Caudovirales phage" /mol_type="genomic DNA" /isolation_source="healthy skin from forearm of human male" /db_xref="taxon:2100421" /clone="9F_4" /environmental_sample gene <1..776 /locus_tag="9F4_1" CDS <1..776 /locus_tag="9F4_1" /note="Similar to PF03527.9 [RHS] (hmmscan score : 59.3)." /codon_start=3 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72577.1" /translation="SKAHCPPSPGAGPAWIEQQQRTLNAQYDCGRNIYGWDGDTLAYE TRYSDNPGERRLIHYFYEPGSFVPVAQTVENRSLSLVREPSHENGYHIDRDPLWQHQP VAKPFNAIAWYQCDRLGTPMELTDQRGEIAWSATYQAWGLAKEKRTDNAIRENIRNPL RFQGQYFDTETGLHYNRYRYYDPQVGRFISKDPIGFAGGLNFYAYAPNPVGWVDPFGL NRTNKQGHYHGPKPKYHNPGHHDLSSGNFRGGTTLANDV" gene 848..1552 /locus_tag="9F4_2" CDS 848..1552 /locus_tag="9F4_2" /note="Similar to PF03527.9 [RHS] (hmmscan score : 58.5)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72578.1" /translation="MREPSHENGYHIDRDPLWQHQPVAKPFNAMAWYQCDHLGTPMEL TDQRGEIAWSGTYQAWGLAKEKRTGSAIRENIRNPLRFQGQYFDTETGLHYNRYRYYD PQVGRFISKDPIGFAGGLNFYAYAPNPVGWVDPFGLARIPKSVKQKISDENRDFFGVE TCESCISAVVPGQKSTKGISPPSNERQFDHIEADSRGGSNDENNTQLLCRKCNRGFSN NSKPNFRILNRIDGVQ" gene 1552..2061 /locus_tag="9F4_3" CDS 1552..2061 /locus_tag="9F4_3" /note="Similar to PF14085.1 [DUF4265] (hmmscan score : 88.7)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72579.1" /translation="MTSQSLKKITFKLNQDMEDYPPLEFESLWGIQIKPHTYKVDNIP YYIYGVSKGDIILAIESNKQIQASYLLYRGGHSTLRVFAENKSDRIKIINYTKKNGAQ CTSQKGSSLLAIDIPPSIDFHKIDAFLSNQSDNKTIAYEDACLQHTISDINRINECYS LSTIPIWTN" gene 2369..2746 /locus_tag="9F4_4" CDS 2369..2746 /locus_tag="9F4_4" /note="Predicted by MGA" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72580.1" /translation="MASRCQCLQVLRQTFALYPASSLRDYVSQHYTHDARGNLLERLD NGKKARFTWDLYDRLTRYEDDRLTADFAYDALGRRVAKYSKAHCPPSTGAGPAWIEQQ QRTLNTQYDCGRNIYGWDGELGL" gene 3059..3244 /locus_tag="9F4_5" CDS 3059..3244 /locus_tag="9F4_5" /note="Predicted by MGA" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72581.1" /translation="MSISTGIARKLCGVFSFKRTTETRRLSRDEASLIEHYRMLSESD RIAMRYLCTSLKEISRF" gene complement(3964..4521) /locus_tag="9F4_6" CDS complement(3964..4521) /locus_tag="9F4_6" /note="Predicted by MGA" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72582.1" /translation="MTIRPLLYGWIFYLALAWLTSIGIFYSDIAIHNDLHRFLPVIER AEAYFVENNELPFLKKTAWIYEKDLALFVMMLLWGGICSRYFLKVNFVGVLEFARYKE NALGIIMVLACVFAILSNIFGPGYEDSTRGLFSNKLYLFSTIFGAFNYTVTLPLLFPL IAFGVSGFFSPIDKIDHELTNLNNK" gene 4743..6380 /locus_tag="9F4_7" CDS 4743..6380 /locus_tag="9F4_7" /note="Similar to PB000132 [Pfam-B_132] (hmmscan score : 191.1)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72583.1" /translation="MSLSAEQIEQAKSISDLLTAMGSSMANSSNSKIQALGESLAHSG DIGGILSDLAKLEASSADFQQMLNSAAEVAIGMTGSAGGVAALESFLISSIPRALAVG ALTAAFPVVGTLALGAAYLGAAYAGGKLIEGAYNWAKDAAETLSSQLSSGTVELPDSF DPKNHPNALVLDATEVNAEYNGYDTDQNSTQYLLDAARREDREFLVQMQKEIDGFNFS LSSLVKNDTGFLYPNSYGMMSWNGGYAGIQNYVIDGMGLNIDLNSNWRTELDFKINGL LESAYSMQEFVNNNIDRADSALGGEGKSFSNSAMQSVMEAAEVMHEITPLALDLSGDG ILTRSIFDGIKGFEFIQGEEGAYHGWLDANSGFLAIDRNGNGIIDNGTELFGSVNEDG FSALRRLDSNNDGRIDSFDDLFYKLLVWQDKNQNGISEPDEVMSLKEIGIASISLEQV QMVYRPDINGNTIKWISEYTLESGDKREIADVYFSYSKPVSGMTDKPDHDLSNSSTDH KTQALAGNSDSFTPPPSIEIIGQSNANDLLDSPLITI" gene 7150..7434 /locus_tag="9F4_8" CDS 7150..7434 /locus_tag="9F4_8" /note="Similar to PF04221.7 [RelB] (hmmscan score : 57.7)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72584.1" /translation="MAVIVMTIVITGINPMASINIRVDDELKARAYQELERLGVTPSE LMRQALQYVAERGQLPFRPVLMTEEDEQLINTVRERLAAPQRVKVTLDDL" gene 7424..7711 /locus_tag="9F4_9" CDS 7424..7711 /locus_tag="9F4_9" /note="Similar to PF05016.9 [Plasmid_stabil] (hmmscan score : 48.4)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72585.1" /translation="MTFSLEFDQRALKEWHKLGDTVRQQFKKKLAEVLLNPRVEANRL RDLPDCYKIKLRSAGYRLIYQVIDQEVVVFVVAVDKRENEAAYRKAEDRLK" gene complement(7937..9031) /locus_tag="9F4_10" CDS complement(7937..9031) /locus_tag="9F4_10" /note="Predicted by MGA" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72586.1" /translation="MKDRGEAVFVFSGAHISEMSPMDQEYASAASERTRLMVELCGRN TMISFDRLMKAELACLATQDHHSVYALDDNGEWFPSMGTLISPIDELDIPGALQKQVE LQKDEHGMNRKMRRMLKAHTTNKNGSFRSDIEQRFGQRLDYRELLERTPMRPQDMTVF KNYLLGKATREEANKAFLESLRDPTYMAQWFIHHYHKLGAIGEWVRRPARELIESCEQ TLSNLRVQLETLPASERAAVMAGVSGERWVKLKKQSMIDILNRLLVRFLPGAPRCDDV GKIQKYCPGIFICINAFFDSLQKSFGEIPRAMKASDFVDAIHALYIPYVSYFRADKHM CNILQPLAQHFGTQIVSSPAKLTAALGVAF" gene complement(9372..10001) /locus_tag="9F4_11" CDS complement(9372..10001) /locus_tag="9F4_11" /note="Predicted by MGA" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72587.1" /translation="MEHALTYADVHRVKRLAKQLKKTNPELSHAKRLDQAATELCGAR NFYELNRWYDRQINKQVDTPEGSQSVSHCLYCDYRFAADHKPDQKSHRDIHERFMEVE EKLGYRPGTYVERERMKLDGYEEMRRKMHDAEEVDGLLMITRSWFDRSLHSAVTGGYW VKHPDFEAYVAMMIPQLETMNSAQAAVLASRFGRTSGVIPKGGTYWSPN" gene complement(10437..10958) /locus_tag="9F4_12" CDS complement(10437..10958) /locus_tag="9F4_12" /note="Similar to YP_001039848.1 [Vibrio phage VP882] (BLASTp score : 204)." /codon_start=1 /transl_table=11 /product="putative DNA adenine methylase" /protein_id="ASN72588.1" /translation="MARPETLTDIQRAARFFYLQHHAFGGKVSGQTFGTATTGPAINL MRIEENLSAAWQRLVGTYIENLPWLECAERYDRPHTFHYMDPPYWQTAGYGVDFPFEN YERMADFMRRCKGKVMVSINDHPDIRRVFEGFHFEMLDIRYCNTNRRQVKAEVSGELV IMNWEPMALGGLF" gene 10893..11225 /locus_tag="9F4_13" CDS 10893..11225 /locus_tag="9F4_13" /note="Predicted by MGA" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72589.1" /translation="MVLQVEESRRALDVGQGFRAGHFLPREYLPGAEGPFELAHEFFQ VVLYDAVEVYQFAVDVVEYFDWSRLWAHKEERSTACKHFDITFMRWEKWEQTVGQTAF AAHPRDDG" gene complement(11382..11909) /locus_tag="9F4_14" CDS complement(11382..11909) /locus_tag="9F4_14" /note="Similar to PF03245.8 [Phage_lysis] (hmmscan score : 63.1)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72590.1" /translation="MNFEAVKWGGALAITLALVIGSAWVTWKWQANAYDKELAIQEAT HQTVLTHLANANSALILEEQGKRFALEQWLAASDQSHYRALSDEKSNQTRLRDRLATA DLRLSVLLDSTAATDCDRMQAATGPGGMVHGAYRAQLDRAHAQRIIGITGDGDQGLIA LQACQAYAKEVSSTQ" gene complement(11906..12451) /locus_tag="9F4_15" CDS complement(11906..12451) /locus_tag="9F4_15" /note="Similar to YP_009043563.1 [Pseudomonas phage phiPSA1] (BLASTp score : 239). Similar to PF00182.14 [Glyco_hydro_19] (hmmscan score : 56.9)." /codon_start=1 /transl_table=11 /product="putative glycoside hydrolase family 19 protein" /protein_id="ASN72591.1" /translation="MSITRQQLLQILPNAGQFAGVFVPVINTAMVRYQIIGPQRTAAF IAQIGHESGQLRYVRELGGNHYLSKYDTGSLAQRLGNTPEADGDGQKYRGRGLIQITG RTNYQACGEALGIDLLNHPELLEQPQYACLSAAWYWATNGLNTLADAGHFDTITRRIN GGQNGAAERKALYARALKVLA" gene complement(12509..13546) /locus_tag="9F4_16" CDS complement(12509..13546) /locus_tag="9F4_16" /note="Similar to YP_007112505.1 [Vibrio phage vB_VpaM_MAR] (BLASTp score : 189). Similar to PF05954.6 [Phage_GPD] (hmmscan score : 143.9)." /codon_start=1 /transl_table=11 /product="putative tail protein" /protein_id="ASN72592.1" /translation="MTPTFRIVADGADITSLINDRLLLLRTTDKPGMESDDFELRVDD RDSAVSLPSRGASIEIFLGYIGTALTRLGRYTVDEVELSGPPDTLVIRGKASDMRGSG KTTRSGSWEGVSLGSIVNDVAARNGWLPICPVTTEVPRADQLSESDFNFITRLAKQYD CTAKVADGMLLVLPRQAGQSASGKLLGAVTLTRSDVSRWQFRLADRSAHKAVITKHQD PNTGQLQIITINNEDAPEGLPPVHTDRHIYPNKSAAEQAAKARLTAFNRSTAAVRLEM PGRTDLFAERSINAQGFKAGLDGQYLVDSVEQVFTQSGWSTTVECNGGKQGKAKTSGL KPVVPLKIVDL" gene complement(14021..15217) /locus_tag="9F4_17" CDS complement(14021..15217) /locus_tag="9F4_17" /note="Predicted by MGA" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72593.1" /translation="MSDQQIVEAVENGDLLLVDAAPFSPLSDDNLGRYAHITGQGRPG SSHSPVEREREPLPVSKPEFRQPLQEPGFYVVRKSTTVDQLKAALFSSQYPAVMSKFD ALNPGLSTVNAGSMIVLSDPNNLQCTREEAILMAAAAKANEAVKALSPEEADFMARHR DEIETVIARGATAIGIGEAMFAKNLDDVRKLFKEVEALHIRSFQTDGHLRSPAFFAER RRLLAQLNTNLTTLTRKSIGFPDHPNLKSALGISSRSLVHHWTLAGGADQIPGYATHI DSVSKASKYLKLGGWIGTGIGGGASYMKVQDVCSAGNTEACKRIKFTEAGSFLGGAAG GAVAGAVLTGSTVGYVCFAIGVPTGGVGTLICGVAVVGVASFASGAIGGVIGEETGDV IYEIVK" ORIGIN 1 actccaaagc ccactgcccg cccagccccg gcgccggccc ggcgtggatc gagcaacagc 61 agcgcacgct caacgcccaa tacgactgcg gccgaaacat ctacggctgg gacggcgaca 121 ccctcgctta tgaaacacgc tacagcgaca accctggcga acgacgtctg atccactact 181 tctacgaacc gggcagcttc gtccccgtcg cccaaaccgt cgaaaaccgt agcctgtcgc 241 tcgtgcgcga accaagccat gaaaacggct accacatcga ccgcgacccg ctgtggcaac 301 accaacccgt ggcaaaaccg ttcaacgcca tcgcctggta ccaatgcgac cgcctcggca 361 cgcccatgga actcaccgac caacgtggcg agattgcctg gagtgcgacg tatcaagcgt 421 gggggctggc caaagagaag cgtaccgaca acgcaatacg cgaaaacatc cgcaacccgc 481 tgcgctttca agggcagtac ttcgacaccg aaacagggct gcactacaac cgctatcggt 541 actatgatcc gcaagtcgga cggtttatca gcaaggatcc gatcggtttt gcgggtgggc 601 tgaatttcta tgcgtatgcg ccgaatccgg tggggtgggt tgatccgttt gggttgaacc 661 gtaccaataa acaaggacac tatcacggac caaaacccaa atatcataat cccggccatc 721 atgacctttc atcaggaaac ttcagaggcg gcacaaccct ggcgaacgac gtctgatcca 781 ctacttctac gaaccgggca gcttcatccc cgtcgcccaa accgtcgaaa accgcaacct 841 gtcgctcgtg cgcgagccaa gccatgaaaa cggctaccac atcgaccgcg acccgctgtg 901 gcaacaccaa cccgtggcga aaccgttcaa cgccatggcc tggtaccaat gcgaccacct 961 cggcacgccc atggaactca ccgaccaacg cggcgagatt gcctggagtg ggacgtatca 1021 agcgtggggg ttggccaaag agaagcgtac cggcagcgca atacgcgaaa acatccgcaa 1081 cccgctgcgc tttcaagggc agtacttcga caccgaaacc gggctgcact acaaccgcta 1141 tcggtactat gatccgcaag tcggacggtt tatcagcaag gatccgatcg gttttgcggg 1201 cgggctgaat ttctatgcgt atgcgccgaa tccggtgggg tgggttgatc cgtttgggtt 1261 ggctcgcatc ccgaaatcag ttaaacaaaa aatttcagac gaaaataggg atttttttgg 1321 agtggagaca tgtgaaagtt gcatttccgc agttgtacca ggacaaaaaa gtacaaaagg 1381 cattagcccg ccatccaatg aacgtcagtt tgaccatatc gaggccgact cacgcggtgg 1441 ctcaaatgat gaaaacaata cacaactttt atgtagaaaa tgcaaccggg gcttctcaaa 1501 caattcaaaa ccaaacttca gaatactaaa tcgtatagac ggcgttcaat aatgacttct 1561 caatcactca aaaaaataac attcaaacta aaccaagata tggaagacta tccaccactc 1621 gaattcgaat cattatgggg aattcaaata aagccacaca catacaaagt agacaatatc 1681 ccctactaca tttatggcgt aagcaaagga gatatcattt tagctataga aagcaacaaa 1741 caaatccagg cgtcgtacct tttatacaga ggtgggcact caacgctacg cgtatttgct 1801 gaaaacaaaa gcgatcgcat taaaatcatt aactatacaa aaaaaaatgg cgctcagtgc 1861 actagtcaaa aagggagctc actacttgct attgatatcc cccccagcat agattttcat 1921 aaaatcgatg ctttcctttc aaatcaatca gacaacaaaa ctattgccta tgaagatgcc 1981 tgcctgcaac acactataag tgacataaat cggattaatg agtgttactc actctcaacg 2041 atcccgatct ggacaaattg atatttacac tgcattttga gcttattact gatggcattc 2101 ggatagttca atgaacgaat ctatcctcac acgcaggctt caatatgaag acaataacag 2161 actaaggtta cctggtgacg catgtcactt gtataaaagg gcatatccaa tatttataaa 2221 aaaataccta tcccgtcatc gatgtatggg gagcactgca agttatgaag accttcgcgg 2281 taccggactg atcaccagtg actcacaagt atggttgcag cgctgaccat aaagttcata 2341 gctctttaac agtcgggaaa caccggcatt ggcctcccgc tgtcaatgcc tgcaagtatt 2401 aagacaaacc ttcgccctct accctgccag cagtctgcgc gactacgtca gccagcacta 2461 cacccacgac gcccgcggca acctgctaga acgcctcgac aacggcaaaa aagctcgctt 2521 cacctgggac ctctacgacc gcctgacccg ctacgaagac gatcgtctga ccgccgactt 2581 cgcctacgac gcactgggga gacgggtagc caaatactcc aaagcccact gcccgcccag 2641 cacgggcgcc ggcccggcgt ggatcgagca gcaacaacgc acgctcaaca cccaatacga 2701 ctgtggccga aacatctacg gctgggacgg cgaacttggc ctatgaaagc cgcaacggcg 2761 aaaccacttt gcgacaacgc aaaaaaaagt gttttccact gtagacggct atagccccac 2821 acttgacgag gctgcacatc gaaaagaggg ctttggctaa ttagtcagcg ttcttgctca 2881 ataggctcta tcaaagaagc taaatgatta ggtaaacaca atcaccggat ctgaccatgc 2941 aggaagagaa ctggctctac actttaggca atgccacttg cttgcttgga actgatttct 3001 gagcaggatc aaagagcagc aactctgggg atgttctttc tggcttaagg tgtgaattat 3061 gtcgatttca actggcattg cgcggaagct gtgtggcgtc ttctctttca agcgaaccac 3121 tgagactcgg cggctcagcc gcgatgaagc cagcctgatt gagcattacc gcatgctgtc 3181 tgagtcagac cgcattgcga tgcgttacct gtgtacttcg ctaaaagaaa tttcccgatt 3241 ctaaaacgcg gggatgccta tggactgctg agctacacac atagagacat cgaacgagat 3301 tgtgggggcg gcgagaaatc gcaacgagga ggaggctgac tttgaggcac ctctgattgc 3361 ggaaacgatg gccgccctgc agccccattt cccaaacccc agaaacgaca aagcccgcta 3421 aaagcgggct ctgtcggtgt agcaatatgg cggaggcgta gagattcaaa ctctaggacc 3481 tgttacagtc gacggttttc aagaccccta tgcatatata tacagcacgg ggcttgtggg 3541 atatttcaag taccaatact tcaagggtgg cgattggctg gaagccgcgt tcggtgcggg 3601 gaaatttttt ggttttggga ctacttttac cctatcaatt gctcacaagg attaggagta 3661 gttgtgccca ggaagctaca acggatttgc aatccgtacc cctaaaacat cagcatccat 3721 tcatgattta tctcatcaaa gaactgcgtg atatccgcat ccaatatcca gttcaccttt 3781 tagcccttca gcgcgatcgt caacgcatac agcgggtcgt gatggctaca tccctatcaa 3841 aactataata ccccccagaa tgcgctcatc ccaagatgct aggataatat aactttcata 3901 cacccagggg atgtgaaact attagcttat agcgtagcgg aaccccctta aagaaaagga 3961 cttttattta ttatttaaat tcgtcaactc atgatcgatt ttatctatcg gactaaaaaa 4021 accggagacc ccaaaagcaa ttaaagggaa aagcaacggc aatgtaactg tgtagttaaa 4081 cgcgccaaat atcgtgctaa aaaggtacaa cttattagaa aacaaacctc gagttgaatc 4141 ctcatagcca gggccaaaaa tattagacag tattgcaaaa acgcaagcca agaccatgat 4201 tataccgaga gcattttctt tgtatctcgc aaactctaac accccaacga aattcacttt 4261 aagaaaatat ctagaacata tacctcccca taacaacatc atcacaaaaa gagctagatc 4321 cttctcataa atccatgcag ttttttttaa aaacggaagt tcattattct caacaaaata 4381 agcttctgct ctttcgatta caggtaaaaa tcggtgcagg tcattatgaa tagcgatgtc 4441 actataaaat atcccaatac tagtcaacca cgctaaagcg aggtagaaaa tccagccgta 4501 caaaagcggt cttattgtca tctgttgtat ttaccctaat gtgagtgagc ttttttgttg 4561 catcgacatc aactaaacag aaacgcccct attgctaatg ataaccatta ttgatatggt 4621 ggcacaaaaa aatgagaacc tctagcataa tcctgcaaaa aaattatcag aaccctagaa 4681 actcgccttc tctgaggcat agccaacaga ccaggcaaga atcagtacaa ggagtacaag 4741 atttgagttt atcagcggaa caaatcgaac aagccaagtc tatttctgat ttactgactg 4801 ccatgggcag ctctatggca aatagctcta actctaagat tcaggctttg ggggagtcgc 4861 ttgctcactc aggtgatatc gggggaattt tatcagatct agcgaaactt gaagcttctt 4921 ctgcagattt tcaacaaatg ttgaacagcg ccgcagaagt tgctattggg atgaccggta 4981 gcgctggagg ggttgctgct ctggaaagct tcctaatatc aagcatacct agagcactag 5041 ctgttggggc attaaccgcc gcatttccgg ttgtaggaac attggcctta ggcgcagcat 5101 atttaggtgc agcttatgca ggaggcaaat taattgaggg ggcttataac tgggcaaaag 5161 atgctgctga aacattatca tctcagttat cttcgggaac tgttgagcta cctgatagtt 5221 tcgaccctaa aaatcatccg aatgctctag tgcttgatgc gactgaggta aacgctgagt 5281 ataatggcta tgataccgat caaaacagca cccaatatct acttgatgca gcacggcgtg 5341 aggatagaga gttccttgtg caaatgcaaa aagaaattga tgggtttaat ttctccctat 5401 ctagtttagt caagaatgat acgggttttt tatatcccaa tagttatggc atgatgagct 5461 ggaatggagg atatgcagga atacagaact atgtgattga tggaatgggg cttaatattg 5521 atcttaattc caactggaga actgagcttg attttaagat aaacggactt ctcgaatctg 5581 catactcaat gcaagaattc gttaacaata atatcgatcg cgccgactca gcccttggag 5641 gtgaaggaaa gagcttttct aatagcgcaa tgcaatctgt aatggaagca gcagaagtta 5701 tgcacgaaat aacgccacta gcgctagatt taagtggaga tggaatcctg acgcgttcaa 5761 tatttgacgg catcaaaggc tttgaattta ttcagggcga agaaggagct taccacgggt 5821 ggttggatgc taacagtggc tttcttgcaa tagacagaaa tggcaatggt attatagaca 5881 atgggaccga gctgtttggc agtgtaaatg aagacggatt ttctgctttg cgtcgacttg 5941 actctaacaa tgacggccgt atagattcat ttgatgatct attttataag ctattggtgt 6001 ggcaagataa aaatcagaat ggcatatctg agcctgatga agtgatgagc ctaaaagaaa 6061 ttggtatcgc atcgatttca cttgaacagg tccagatggt ttatcgccca gatataaatg 6121 gcaatactat caaatggatt tccgagtaca ctttagagag tggtgataaa cgcgagattg 6181 cggatgttta tttcagctac agcaagccag tttcgggcat gactgataaa ccagaccatg 6241 atttatctaa ctcttcaaca gaccataaaa ctcaggcgct agcaggcaat tcagattcat 6301 tcaccccccc tccaagtatc gagataatag gtcagtcaaa cgccaacgac ttattagaca 6361 gcccgctgat cactatttaa ttcacaacta ttagccccta actctagaac ctagaaagca 6421 agttgaaata ttctatgaaa aaatacactc ctttgcaaag tatgccataa tttttttatg 6481 atatctcact cattagtgat gcgttttagt tcgtactaac aggcgacgca gttcagtgtt 6541 ccgacatagt tctgctgccg ttaattttga agttttcgga agcgctttat ccagacatag 6601 aggctataca tcgacacacc gagccgctat atcagcgaca gacagcttct tttcgataac 6661 tactcgacca cttagatttt aaatctcttc gggttcaggc tggttggtta tggtacctcc 6721 taatgggcct cattttaagg ctaagaggtg tctacgaaac taggggcgat tcacccctcc 6781 caaaacaagc agagttatgg aacacatgct aaaaagtttt ggaacgtttc acccaaaacc 6841 tcgcttgagc caccccttgc cccaaacccc agaaacgaca aagcccgcta aaagcgggct 6901 ctgtcggtgt agcaatatgg cggaggcgta gagattcgaa ctctaggacc tgttacagtc 6961 gacggttttc aagtctcgaa taaaatcgtt atatttcaat aggttagtat tcatcatatt 7021 ccgcaagatg aatattcttg cagagctata ggccgcgtat ttcaaggctt ggatttttaa 7081 ttgcggaaag gattttgaag caataacacc tctcttggct ctgccgaacg gcacttgcaa 7141 aaaaaggtta tggcagtcat tgtaatgaca attgtcatta caggaatcaa ccctatggcc 7201 tccatcaaca tccgcgtaga cgacgaactc aaagcccgcg cctaccaaga acttgaacgc 7261 ctcggcgtta cgccttcaga gctgatgcgc caggccttgc agtacgttgc agaacgcggc 7321 caactgccct tccggcccgt gctcatgacc gaagaagatg aacagctcat caacaccgtc 7381 cgcgagcgtc tcgcagcacc acaacgcgtg aaggtgacgc tggatgacct ttagtcttga 7441 gtttgaccaa cgcgcactca aagaatggca caaactcggg gacacggtca ggcagcaatt 7501 caagaaaaaa ctcgcagaag ttctgctcaa cccacgggtt gaggctaacc gactgcgcga 7561 tctgcccgac tgctacaaaa tcaaattgcg cagtgcgggt taccgcctga tctaccaggt 7621 gattgatcaa gaggttgtgg tgttcgtggt cgctgtcgac aagcgtgaga atgaggctgc 7681 gtatcggaag gctgaggatc ggctgaagta ggtgaatatt aattatcgtt tgcggttttc 7741 aaacatcgaa caaagtcgtt atattccaat aagtcagttt tttatattcc gcaatacgat 7801 tattcttaca gagccacaga ccgcgtattt caaggcttga ttttttgatt gtggaaaggg 7861 cgttgaagca acaacactct gttctgccga ataatgctgc aaaaaattaa tttatccccc 7921 cagaactcgt taaggctcag aacgctacgc ctagtgctgc ggttagcttg gcaggtgacg 7981 atactatttg ggtaccaaag tgttgggcaa gcggttgaag gatgttgcac atatgtttat 8041 cagccctgaa atacgacacg taaggaatat atagcgcatg gatggcatct acgaaatcac 8101 tcgccttcat cgcccgcgga atttctccaa atgacttctg tagtgagtcg aagaacgcat 8161 tgatgcagat gaatatgcct gggcaatact tctgaatttt gcctacatca tcacaacgag 8221 gagctccagg taaaaaacga acaagtaacc ggttgagaat atcgatcatg ctctgctttt 8281 taagcttcac ccagcgctcc ccacttacgc ctgccattac tgcagcacgc tcgcttgctg 8341 gcaatgtttc gagctgcacg cgtaggttag aaagcgtctg ttcgcagctc tcaatcagtt 8401 ctcgcgctgg tcgcctgacc cactctccaa tagctcccag tttgtgatag tggtggataa 8461 accactgagc catgtaggtg gggtctctga ggctttccaa gaaagctttg ttagcctctt 8521 cacgcgtagc cttccccagg aggtagttct tgaacactgt catatcttgg gggcgcattg 8581 gcgttcgctc aagaagctca cgataatcca gtcgttgtcc aaagcgttgt tctatatcac 8641 tgcgaaaact gccgttctta tttgtcgtat gagcctttag catcctccgc atttttcggt 8701 tcattccgtg ctcatctttc tgcagctcta cctgtttctg cagcgctcct ggaatatcca 8761 gttcatcaat tggactaatg agcgtcccca tgcttggaaa ccattcacca ttatcatcca 8821 atgcgtacac agaatgatgg tcttgcgtgg ccaaacacgc gagttcagcc ttcattagcc 8881 gatcaaacga gatcattgtg ttacgaccgc agagctccac cataagtctc gtccgctcag 8941 atgcggcaga agcgtattcc tgatccatcg gcgacatttc tgaaatgtgc gctcccgaga 9001 acacgaagac tgcctcaccg cgatctttca tcgccctgag agcggccaaa cttcgcgcac 9061 ccgcatcgtc aactccgggc ctgctaaggt tagagtagtc gcagctatct aagtagatca 9121 atggtggtgt gcgtgtactc aatgattcac cctagctttt ttagaaatgt acatgatgaa 9181 catgaacaac ctgacagaag gtggctaaca acctcaactc cgacacaaac ctatcacagc 9241 ccgcgacacc acgctgtggt actcgatgct gtacgctaca cgaaacgaca ttatcggctc 9301 ttagccctga ttgacggcgt tgaaacaaaa agcctggaac gagtaacgaa ccaggcgctt 9361 gtaaagcaag gtcagttagg agaccagtaa gtgcctccct taggtattac tcccgatgtc 9421 cgcccaaagc gtgatgccaa tacggcggcc tgggcagagt tcatagtttc cagttgggga 9481 atcatcatgg ctacataggc ctcgaagtcc ggatgcttaa cccaatagcc gcccgtgact 9541 gcgctgtgaa gcgagcgatc gaaccagctc cgagttatca taagcaaccc atcaacctcc 9601 tccgcatcat gcatcttccg acgcatttcc tcgtacccat ccagcttcat ccgctcgcgc 9661 tccacatagg tgccaggacg gtaccccagt ttctcttcga cctccataaa gcgctcgtgg 9721 atatcccggt gcgatttttg atccggcttg tgatcggcag caaaacgata gtcacaatac 9781 aaacaatgac tcacgctttg agatccttca ggtgtgtcca cttgtttatt aatctgtcgg 9841 tcgtaccaac gattcaactc ataaaaattt ctagctccgc acagctcagt agcagcctgg 9901 tccaagcgct tagcatgtga cagttcggga ttagtttttt tgagttgctt tgcgagacgc 9961 ttgacgcgat gaacgtcagc ataggtaagg gcgtgttcca tcttagcctc cagggctggt 10021 tctagtgtcc actacgagaa cccaatacag ggccggtcag tactgtcacg gtagagcgta 10081 agctttgcct ttgtggacgg caggcaccta ccgggtgcct ttgcataggc tatgacaatc 10141 aacccctcat atgcaagtct tcaatgtgcg caaactcaaa acttgaagcg actcatacga 10201 aggagcatta atcggctacg atcagttaat tgagtgataa acagaaggtg gctaggctgt 10261 agcgttttct aaaaaaaaag cgcaacagcc ctgaaacaaa gaactgcagg gtttaattga 10321 caactaagaa tacttgttcg cttggcatta aatggcattg attggcgtac agtttgcccc 10381 atttttgttc catccaccgt cgatccggcg cacgctcaat tactgcatac cccccgtcag 10441 aacaaacctc ccagagccat aggctcccag ttcatgatca ccaactcgcc gctgacctcc 10501 gcctttactt gtcgccggtt ggtattgcag tagcggatgt ccagcatctc aaaatgaaac 10561 ccttcaaaca cccgccgaat gtccgggtgg tcgttgatgc tgaccatcac cttgcctttg 10621 cagcgacgca taaagtcagc catgcgctcg taattttcaa acggaaagtc gacgccgtac 10681 cctgccgtct gccagtaagg cgggtccatg tagtggaagg tgtgcggtcg gtcgtagcgc 10741 tcggcacact caagccacgg caggttttca atgtaggtgc ctacgaggcg ttgccaggcg 10801 gctgagaggt tttcttcgat gcgcatcagg ttgattgccg ggccggttgt tgcggtgccg 10861 aaggtctggc ctgaaacctt gccgccaaag gcatggtgtt gcaggtagaa gaatcgcgcc 10921 gcgcgctgga tgtcggtcaa ggtttccggg cgggccattt tttgccacgc gaatatctgc 10981 cgggagctga gggcccattt gaattggcgc acgaattctt ccaggtggtt ttgtacgacg 11041 cggtagaggt ttatcagttc gccgttgatg tcgttgagta cttcgactgg agcaggttgt 11101 gggcgcataa agaagagcgc tccaccgcct gcaaacactt cgacataaca ttcatgaggt 11161 gggaaaagtg ggagcaaacg gtcggccaga cggcgtttgc cgcccatcca agggatgatg 11221 ggtgaagtca ttgtgtgcaa gacctttatg tttttgagat tcaggtgcta ggctcgctgc 11281 gctttgtgca cagagcggga gccttggctg ggcttgcagc tctattttgc gggttcggtg 11341 gccgggctgg atgttgacgc atccagtgtc ggccgctctt tttactgtgt gcttgagact 11401 tcttttgcgt aagcctgaca ggcctgcagg gcgatcagtc cttggtcgcc gtcgcctgtg 11461 attccgataa ttcgttgagc atgcgctcgg tcaagttggg cgcgataggc tccatgaacc 11521 atgccgccgg ggccggtggc ggcttgcatt cggtcgcaat cagttgcggc ggtgctgtcg 11581 agtaggactg acagccgcag gtcagcagta gcaaggcgat cacgcaggcg ggtttgattg 11641 gatttttcat cgctcagggc tcgatagtgg gattggtcgc tggctgccag ccattgctcc 11701 aaagcgaagc gcttgccctg ctcttcgagg attaatgccg agttggcgtt ggccaggtgg 11761 gtaagtacgg tttgatgcgt ggcttcctgt atggccagct ctttgtcgta ggcgtttgcc 11821 tgccacttcc acgtcaccca cgcgctgcca atcactaggg ctagggtgat ggccaatgcc 11881 cctccccact tcaccgcctc gaaattcacg ccaacacctt taacgcccgg gcgtacaggg 11941 ctttgcgttc ggcggcgcca ttctggccgc cgttgatgcg ccgcgtgatg gtgtcgaagt 12001 gcccggcatc ggccagtgtg ttcaggccgt tggttgccca ataccacgct gcggacaaac 12061 acgcatattg cggctgctcg agcagctcgg gatggttgag caaatcaatg cccagggctt 12121 cgccgcacgc ttgataattg gtgcggccgg tgatttggat caggcctcgg ccacggtatt 12181 tctggccgtc tccgtcggcc tcaggggtat tgcccaagcg ttgggcgagt gagccggtgt 12241 cgtatttgct caggtagtga ttaccgccca attcgcgaac gtatcgaagc tggcctgatt 12301 catggccaat ctgggcgata aacgcggcag tacgctgggg gccaatgatc tggtagcgaa 12361 ccatggcggt gttgatgacg ggtacaaaaa cgccggcgaa ttggccggcg tttgggagga 12421 tctgcaggag ttgttgccgc gtaatcgaca tggggttttc ctaacgaaaa aacccgctca 12481 gggcgggttg ggtttggggt tttaatggtt agaggtcaac gattttcagc ggcactacag 12541 gcttcaaacc actggtcttg gctttgcctt gcttgccccc gttgcattca acggtggtgc 12601 tccagccgct ttgggtaaag acctgctcta ccgagtccac caggtactgg ccatcgagcc 12661 ccgctttgaa gccttgggca ttgatgctgc gctcggcgaa caagtcggtg cgcccgggca 12721 tttcaagccg cacggcagcg gtcgagcggt tgaaggctgt cagccgtgct ttggccgctt 12781 gttcggcggc ggatttgttt gggtaaatat gccggtcggt gtgcacggga ggcagcccct 12841 caggcgcgtc ttcgttgttg atggtgatga tttgcaactg accggtgttt ggatcctgat 12901 gtttggttat cacggctttg tgggcgctgc gatcggctaa acgaaactgc caacggctta 12961 cgtcgctgcg ggtcaatgtg actgcgccca agagcttgcc gcttgcgctc tgccccgctt 13021 ggcgcggcaa gaccagtaac atgccgtcgg ctactttggc ggtgcagtcg tattgcttgg 13081 ccaaccgggt gatgaagttg aagtctgact cgctcagttg atcagcccgg ggcacttcgg 13141 tcgtcaccgg gcagataggc aaccaaccat ttcgcgctgc cacgtcgttg acgatgctac 13201 ccaggctgac accttcccaa ctgccactgc gtgtggtttt gccgctgcct cgcatgtcgc 13261 tggctttgcc tcggatcacc aacgtgtcgg gtgggcctga taattcgact tcatcgacgg 13321 tgtaacggcc caaccgggtc agcgctgtgc cgatgtagcc gaggaaaatt tcgatgctgg 13381 ccccgcgact aggtaacgac actgcactgt ctcgatcatc gacacgcagt tcgaagtcgt 13441 cggactccat tccgggttta tcagtggtgc gcagcaatag caggcggtca ttgatcaacg 13501 acgtgatgtc ggcgccgtct gcgacgatgc gaaaggtggg ggtcacgggc aaactccata 13561 catgaaaaag cccactcgat ggtgggctat acatttaagt gcaacgctgc gctattcgaa 13621 aactttcaca aagtctattt tagcaacccc gactttctaa aaatgaacag taaaatcatt 13681 gcagctaaaa gcactatcga agaccacttt atcagggcca actttcgttt aagacgcacg 13741 ggaaatgcat ccagttcttc gatgctgact tccccacgct ttaagtaaaa ccccggaaat 13801 gtaaccactc cggctatcca ccctaccaat aggagcatcc cccaaggccc accattttta 13861 agtggaatat gaggccgaat agcagaagat ttttttaaat gcgccaacat caaatccatc 13921 tgcgtatacg ccatatgaag tgatgcaccc acgcacataa aaatccctcc aaaaatcaat 13981 aaagagaagg caagaaatat tagatcagca atactcacgt tcacttaact atctcataaa 14041 taacatcgcc tgtttcctct ccgattacgc caccaattgc gccggaagca aaagaagcaa 14101 cacctaccac tgcaacaccg caaatcaatg tccccacacc acccgtgggc acaccaatcg 14161 cgaaacaaac atatccaaca gttgaacctg ttaacactgc gcctgccacc gcaccacctg 14221 cggcgcctcc caaaaaacta cccgcttcag tgaacttaat tcgcttgcaa gcctcagtat 14281 tacccgccga acaaacgtct tgaaccttca tgtacgaagc accaccacca ataccagtgc 14341 caatccaacc accgagctta agatatttag aggccttgga cacactgtca atatgagtgg 14401 cataccccgg aatttgatct gcaccaccgg ccaacgtcca gtggtgaacc aagctacggc 14461 tggagatccc gagcgcactt tttaaattcg gatggtctgg aaaccctata ctcttacggg 14521 tcaaagtggt cagatttgtg ttgagttggg ccagcaaacg cctacgctcc gcaaaaaagg 14581 ctggggaccg taaatgtcca tccgtttgga atgagcgaat gtgcaatgct tcgacttctt 14641 taaaaagctt tcttacatca tccaggttct tggcaaacat tgcctcgcca atacctattg 14701 ctgttgcacc ccgcgcaata acagtttcga tttcatcgcg gtgtctggcc ataaaatcag 14761 cctcttccgg actaagcgct tttaccgcct cattggcttt ggcagcggcc gccatcaaaa 14821 tcgcttcttc gcgggtgcat tgcaggttat tgggatcgct caacacaatc atcgaaccgg 14881 cattgacagt gctcagccca gggttgagtg catcaaactt gctcatcact gcggggtatt 14941 gcgacgagaa cagcgcagcc tttaactgat cgactgtcgt gcttttgcga acaacataga 15001 agccaggctc ctgcagtggc tgcctgaact ctggctttga gactgggagt ggttctctct 15061 cccgctctac ggggctatga gaactgccgg gcctgccttg ccccgtaatg tgcgcatacc 15121 gacccaagtt gtcatcgctt aacggactga agggggcagc gtcgaccagc aacaagtctc 15181 cgttttcaac agcctctacg atttgttggt cactcattgg ccgaccgaac acatcaacat 15241 gaaataagtt aagaagcgta tggggcaaat gcaaagcaag ctcgacgttc gcttttgcaa 15301 aaaatggtga ttctatattt ctgaagctaa ttccgagtcg ttcgcgatct tgtcggcgaa 15361 taaggcgcat gcagtatttc cctaattaaa actgaccgcg ccgattgatg ccggccaacg 15421 tcttgaagag cgcggactgt atcggccagt tctgccaagg atctgtcaga cctttccttt 15481 aattaagaag gctttatcta attcatgctc ggccatttaa aaacgatgcc gtttgcggtc 15541 atcaccccca caacatcacc ccctc //