LOCUS MF417961 14951 bp DNA linear ENV 27-FEB-2018 DEFINITION Uncultured Caudovirales phage clone 10S_15, partial genome. ACCESSION MF417961 VERSION MF417961.1 KEYWORDS ENV. SOURCE uncultured Caudovirales phage ORGANISM uncultured Caudovirales phage Viruses; dsDNA viruses, no RNA stage; Caudovirales; environmental samples. REFERENCE 1 (bases 1 to 14951) AUTHORS van Zyl,L.J., Abrahams,Y., Stander,E.A., Kirby,B.M., Clavaud,C., Farcet,C., Breton,L. and Trindade,M.I. TITLE Novel phages from South African skin metaviromes JOURNAL Unpublished REFERENCE 2 (bases 1 to 14951) AUTHORS van Zyl,L.J., Abrahams,Y., Stander,E.A., Kirby,B.M., Clavaud,C., Farcet,C., Breton,L. and Trindade,M.I. TITLE Direct Submission JOURNAL Submitted (29-JUN-2017) Biotechnology, University of the Western Cape, IMBM, C2, Life Science Building, Robert Sobukwe Rd, Cape Town, Western Cape 7550, South Africa FEATURES Location/Qualifiers source 1..14951 /organism="uncultured Caudovirales phage" /mol_type="genomic DNA" /isolation_source="healthy skin from scalp of human male" /db_xref="taxon:2100421" /clone="10S_15" /environmental_sample gene complement(8..2155) /locus_tag="10S15_1" CDS complement(8..2155) /locus_tag="10S15_1" /note="Similar to YP_001974373.1 [Streptococcus phage PH15] (BLASTp score : 545). Similar to PB015921 [Pfam-B_15921] (hmmscan score : 83.1)." /codon_start=1 /transl_table=11 /product="putative minor tail protein" /protein_id="ASN72594.1" /translation="MADGKIVIDVQVNGHKLTELSDALKRLESEARRSGQGVKTAGDG IQSTGDKALRAGQGFKRAGDRMAEGAKLSETSSNGFRRAGDKIKESSEVASKSGNGFK RAGEKIKESSDLAGRSGDGFKQAGQKVKESSDLAQKSGDGFKQASNKIKSASNEASSG GEGFKQAGHKVKASGEEAKGGGAGFKKAGEDAKAGGDKAGQGAKGFEKIKDAIKNFSV GAVAFKAVSSAMNLVSQSMDKAIDRFDTLQRFPKVMKSLGHSSKDVAASTKLLSEGIE GLPTTLDTVVSTTQKLTSMTGNLKQSTKLTIALNNAFLASGASTEDASRGLQQYTQML SAGKVDMQSWKTLQETMPYALQKTAESFGFAGASAQKDFYSALQDGKITFTDFSKRLI ELNKGTNGFAEMAKKNSEGIKTSFGNIVNAVAKGIANVIAEFDKMSKAVTGKSIAQNL DSIKGAVNSTFNVIISVIRGATPVVKSLVSVLGFLKPVLDPLISVFAGVVSAVLLFKG AMLGLSIIKGIGSLIGTLITSLVSLTSTSLVATGATTGLAGALAALSSGGVFIVVGAI AGLVSWLTQESEETKKAKEKAKEFQQSLDDLHESINKGNEAYKDRRNEIQATAEDNER LVKKIDELNAVENKTAAQKKELASAAETLNSRIEGLNIQYDKATGTINLTTDANRQQI EIAKASAEIEAAHERMVEKSKDRHERKDKEKEE" gene complement(2156..2725) /locus_tag="10S15_2" CDS complement(2156..2725) /locus_tag="10S15_2" /note="Similar to YP_001974372.1 [Streptococcus phage PH15] (BLASTp score : 266). Similar to PF06854.6 [Phage_Gp15] (hmmscan score : 178.5)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72595.1" /translation="MKLNEPLENSFEVNGRTYEVDCSFDLVLDVFEMFDNEVMNNLEK MRTAVLMMTDEALDNPEDIVAVWEYIDEHFLKTKKERVVYDRHGNPMPIAKDEEEDIR LIDFEVDAQEIYASFVQAYNINLFEAQGRLTWPEFIALLNGMPEGTAVSQLVEIRSWK PSKNDSSEYKAKMRRLQNKYRLDGKEGDE" gene complement(2741..3217) /locus_tag="10S15_3" CDS complement(2741..3217) /locus_tag="10S15_3" /note="Similar to YP_001974371.1 [Streptococcus phage PH15] (BLASTp score : 173)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72596.1" /translation="MSISIKLKRNYIPINIGEIELQFDTSLENISRLATLQEEIAERF NKYQLELIERSNNGEFDDLKEGVINKQVIDEAFEMQKKMTEIKYDVLFGDGTFAKLYE RYPDLDALDHAFDEVDTMLGAELDRLGQERAKASGAVAESFVKKAKAKKTKKTSKK" gene complement(3281..3733) /locus_tag="10S15_4" CDS complement(3281..3733) /locus_tag="10S15_4" /note="Similar to YP_001974370.1 [Streptococcus phage PH15] (BLASTp score : 209)." /codon_start=1 /transl_table=11 /product="putative major tail shaft protein" /protein_id="ASN72597.1" /translation="MARQKNALRGHFIAPVTDPKTEPAKEAYKELAKWIEDVDDDTDE ATTSVAYYDGDGTEETTVTSVKGSYTFKGTYDKEDEAMALIAGLKYKLGNDRLVWHKV VDSDGKNQHVGIATVSAIKAGSGAAANYEEFSCKISYNSLPKTTAVVG" gene complement(3737..4147) /locus_tag="10S15_5" CDS complement(3737..4147) /locus_tag="10S15_5" /note="Similar to YP_001974369.1 [Streptococcus phage PH15] (BLASTp score : 210). Similar to PF12691.2 [Minor_capsid_3] (hmmscan score : 143.4)." /codon_start=1 /transl_table=11 /product="putative minor capsid protein" /protein_id="ASN72598.1" /translation="MKSNNDFNVVLRDFINTLGLPLACELDFLSELDSLVLYPLPGGK VERVYMDGSRDVSLIFEIAVKVKNQVTASECLWEINKALSEFDLVLPSQNNSYIFNNL TTTQPSLNERDEQGFYIYLQDITANLTILNNKGV" gene complement(4144..4506) /locus_tag="10S15_6" CDS complement(4144..4506) /locus_tag="10S15_6" /note="Similar to YP_001974368.1 [Streptococcus phage PH15] (BLASTp score : 170). Similar to PF11114.3 [Minor_capsid_2] (hmmscan score : 132.5)." /codon_start=1 /transl_table=11 /product="putative minor capsid protein" /protein_id="ASN72599.1" /translation="MGINVTIDLSGATKKVSQASERKARLEIADQILSDMEPYVPLLN DPLRTSGHVAGDGSKIIYNTPYARAQFYGGAYNKYRSFSFSKYTTPGTGKRWDLKASA NHGNKWAEVGLRAMGFNK" gene complement(4506..4865) /locus_tag="10S15_7" CDS complement(4506..4865) /locus_tag="10S15_7" /note="Similar to YP_001974367.1 [Streptococcus phage PH15] (BLASTp score : 166). Similar to PF10665.4 [Minor_capsid_1] (hmmscan score : 42.3)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72600.1" /translation="MVVVDKRTLVDSVTISKPTGKKDGWGKEEFSYPILLSPVRFDRN FDGPGSVNNPSGQKNPSFRAPGVIFVYPRYCNVELDSSFRNSIIKDGDDEYIVNKIIP VYEPFNRKVFCYEIEVM" gene complement(4852..5250) /locus_tag="10S15_8" CDS complement(4852..5250) /locus_tag="10S15_8" /note="Similar to YP_001974366.1 [Streptococcus phage PH15] (BLASTp score : 194). Similar to PB008766 [Pfam-B_8766] (hmmscan score : 63.4)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72601.1" /translation="MAYLTHEEYRELGFESTCEFDELLKRAELAIDLFIRHFYEFHDF DKDHKIRKKAVKLAVAYQIQYLDSTGILTAEDKQTISSTTLGRTSVSYGSNNGSRASE TASGYNLSLDAFNTLKSAGFLYSGVDYGRC" gene complement(5259..5453) /locus_tag="10S15_9" CDS complement(5259..5453) /locus_tag="10S15_9" /note="Similar to YP_001974365.1 [Streptococcus phage PH15] (BLASTp score : 58.9)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72602.1" /translation="MAKYKATCNFLIESTDQNFDEGKVYELTTAEAEEINQKTNLAFG EEWLVLVSDSEPVAQEVASE" gene complement(5457..5603) /locus_tag="10S15_10" CDS complement(5457..5603) /locus_tag="10S15_10" /note="Predicted by MGA" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72603.1" /translation="MAEETKTTKTEAVTEQVATVLVKDDVTFTITDPNLVSAFLTSGY EIKE" gene complement(5614..6438) /locus_tag="10S15_11" CDS complement(5614..6438) /locus_tag="10S15_11" /note="putative Gp7; Similar to YP_008240327.1 [Thermus phage phi OH2] (BLASTp score : 345). Similar to PB000647 [Pfam-B_647] (hmmscan score : 180.7)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72604.1" /translation="MTINYASKFDAKVDERFAKEALSTGIINQDFDFTGVDTVKVYSI PTTAMNDYALTGNTRYGTAAELENNVQTLTLTKDRSFTFTIDKRSVQDTNGTLEAGKA LARQLSEVVIPEVDKYRFGKVVAGADTDHVKTGAVTKNNAYEAVLDGQVKLTDALVPE EGRKLHVSPEFYKLIKLDPSFVKNSDLGQEVAFKGQVGVIDGLPVILTPTSRLPENVA FVIAHPIATTSPVKLEDYKIHDNPPGINGYLVEGRIRYDAFVLDSKKKAIYVHKTA" gene complement(6466..7035) /locus_tag="10S15_12" CDS complement(6466..7035) /locus_tag="10S15_12" /note="Similar to YP_002925101.1 [Streptococcus phage 5093] (BLASTp score : 209). Similar to PF06810.6 [Phage_GP20] (hmmscan score : 135.3)." /codon_start=1 /transl_table=11 /product="putative scaffolding protein" /protein_id="ASN72605.1" /translation="MSLKREMLVDAGIEDKDTIERIMAAYGSAIKEARSEVQAENDSL KTQLEQRDQAIKDLQAKEGASEEAKKQLEDLQAQFESYKTDSEANLAQVKKTNAVALA LKDVGAHNSEDLMKFIDLDKIELAEDGKPKLEETISGLKESSPYLFIQKEEPQEPQPK FALGGNPSAGGDSDLSPEEQALFAGFDSI" gene complement(7199..7321) /locus_tag="10S15_13" CDS complement(7199..7321) /locus_tag="10S15_13" /note="Predicted by MGA" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72606.1" /translation="MNKVIRLLEKVLFRKKKTFLDYQKELSKSAFEGFLSGMEK" gene complement(7314..8486) /locus_tag="10S15_14" CDS complement(7314..8486) /locus_tag="10S15_14" /note="Similar to YP_001974361.1 [Streptococcus phage PH15] (BLASTp score : 604). Similar to PF06152.6 [Phage_min_cap2] (hmmscan score : 375.1)." /codon_start=1 /transl_table=11 /product="putative minor capsid protein" /protein_id="ASN72607.1" /translation="MARNKYPVLFNEEQLELRASQVGDIYHQMARDLFDEVIDRLLER GAESLADNPYIWQLERMSQMHMLNEQNLDTIARYSKIGREQLRKVIEDEGFKIYQTTK EQLIDDLGGGDFGNSKHAQELLTGYFEQSHGDISNLINTTLPGIVTDVYRQMVQEVVA RQVVGLVTHDKAVSQTVMKWQEIGFKGFIDRGGHYWKVDNYARTVIKTTVMRSYREMR TMPADELGIDTFYYSKKATAREACAPLQHHIVTYGPAREEHGISILSLADHGYGTAGG CLGINCGHMLTPFVPGINELPELGPDVKNITQEEAIRNANAQSKQRAYERAIRKSKEK LHVAEKLGDQELISKFKTKIRDQQATLRDYIADKPFLHRDYARERYFKPKGGANDE" gene complement(8491..9999) /locus_tag="10S15_15" CDS complement(8491..9999) /locus_tag="10S15_15" /note="Similar to YP_001974360.1 [Streptococcus phage PH15] (BLASTp score : 775). Similar to PF05133.9 [Phage_prot_Gp6] (hmmscan score : 280.8)." /codon_start=1 /transl_table=11 /product="putative minor capsid protein" /protein_id="ASN72608.1" /translation="MSILQSLRNIFKRGKYVMTSQSLGNITEHPKIAINIDEYNRIQK NLKYYQSKWDPIRYRNSNRVDKQRARNHLPIARTACKKIASLVFNEQAEISVANGTTN EFIQTVLLNDRFNKNFERYLESCLALGGLAMRPYVDDDKIKISFVQAPVFYPLQSNTQ DVSSAAIINKSQKTVSKETIYYTLIELHEWTKDGKYTITNELYRSNEKERVGDRVPLS EVYEDLEEEVTLDGLTRPLFTYLKPPGMNNKDINSPLGLSIFDNAKSTIDFINTTYDE FKWEVRMGQRRVLVPDQTVRIGFDHHGDTDLVTREFDPEQNVYEQIDGGKDTPINITD LTTPIRSDDYIKAINEGLALFEMQVGVSPGMFTFDGKSMKTATEVVSENSDTYQLRNS IVSLVDQSIKELVISICEIGKLYSLYSGPIPEMDDITVNLDDGVFVDKNNELDYYAKA LLSGLVSKQYAISKALGLSDKEAAQMLADIKKETAESMELERSTSEVDIYGE" gene complement(10013..11341) /locus_tag="10S15_16" CDS complement(10013..11341) /locus_tag="10S15_16" /note="Similar to YP_001974359.1 [Streptococcus phage PH15] (BLASTp score : 684). Similar to PF04466.8 [Terminase_3] (hmmscan score : 317.2)." /codon_start=1 /transl_table=11 /product="putative terminase large subunit" /protein_id="ASN72609.1" /translation="MISRTTKPTIKLTKEINPKFYKVWRSSKPYNILKGGRNSFKSSV IALLLVFMMIKAITLGQCVEIIIVRKVGNTIFDSVYKKIIWALDKFGMANQFKRTKSP YKIVHRRTGSTFHFYGQDDFQKLKSNEVGKVIAVWYEEAAEFADSEEFDQSNSTFMRQ KHPDYPFVQFFWSYNPPRNPYNWINEWVDSLRTAEKYLIHESSYLDDELGFVTEQMLD EIERIKTNDYDYYRYLYLGEPVGLGTNVYNMDLFKRVDKIPDGERVIGQLFAADTGHQ QSATTCLHAVVTNRSNLYLVDNYYYSPAGKVKKKAPSVLSKELHDFVIKQTQKYPNVP VIEMTIDSAEGALRNQYLEDFGIRWHPVAKKKKIIMTEYVQSLLANGRFYYFPTENNL KYFIEEHKRYQWDEKTVKDDDPKVIKEDDHTCDAFQYMVVDNAQLLRLKA" gene complement(11322..11768) /locus_tag="10S15_17" CDS complement(11322..11768) /locus_tag="10S15_17" /note="Similar to NP_945277.1 [Streptococcus phage EJ-1] (BLASTp score : 126)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72610.1" /translation="MANEENLIPFNERSVSETRELGKKGGIASGKTRRKKANMKKTLE ALLVSKVSNPQLSRVLQDMGFEDDYESALLLVAMQKALKGSSRHMELISKIVNSEGAK DTLDKKEQKARIKSLELENKRKAQALDEAGGGADESILIIDDIPND" gene complement(11797..12399) /locus_tag="10S15_18" CDS complement(11797..12399) /locus_tag="10S15_18" /note="Similar to NP_945276.1 [Streptococcus phage EJ-1] (BLASTp score : 273). Similar to PF01555.13 [N6_N4_Mtase] (hmmscan score : 83.4)." /codon_start=1 /transl_table=11 /product="putative transferase" /protein_id="ASN72611.1" /translation="MRSLLQIPFYVKGGAFYIWHADSEGYNFRGACSDIGWAVRQCLI WNKNTLVLGRQDYQWKHEPCLYGWKEGAAHYFVNDRSLTTIIEDVEELNKMTKAELIE YIERMQANSPTTIINENKPTRNGLHPTMKPLKLIERLVRNSSKKGWNVLDSFNGSGST MIVCEDLGRTYFGMELDPRYVDATIQRWEEHTGQTAVKLN" gene complement(12314..13042) /locus_tag="10S15_19" CDS complement(12314..13042) /locus_tag="10S15_19" /note="Similar to NP_945276.1 [Streptococcus phage EJ-1] (BLASTp score : 182). Similar to PF02195.13 [ParBc] (hmmscan score : 44.9)." /codon_start=1 /transl_table=11 /product="putative transferase" /protein_id="ASN72612.1" /translation="MKIEKINISEITEYENNAKLHPREQIEQIKKSIQEFGNNDPIAI DENNVIIEGHGRYKALQELGYDEVEVIRLSHMDDEQKRAYILAHNKLTMNSGFDIELL NSELESIVNINMEDFGFDYYEPESEVEEDDFEVEETKEPIAKLGDIYQLGRHRLMCGD STDPDQLAKLVDGQQIDLIVTDPPYNVAYEGGTEEALTIMNDSMDNESFRKFLRDAFF AADTVLREGGGVLHLARRFRGLQF" gene complement(13380..13814) /locus_tag="10S15_20" CDS complement(13380..13814) /locus_tag="10S15_20" /note="Similar to NP_945275.1 [Streptococcus phage EJ-1] (BLASTp score : 117). Similar to PB000040 [Pfam-B_40] (hmmscan score : 121.0)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72613.1" /translation="MLFGEVLKNKTKENADNTLKNYRVLLRIAGEEYSPKVTATYSLE PKSAPSSPSRQTEQMVIRRVSAQQELELMASAINRLSDLNLSQILIERYCRVRFRQDK AIYPSLGYSESEYYRLLDRALLEFAEAYKAGELLEYRFLGDN" gene complement(13815..14198) /locus_tag="10S15_21" CDS complement(13815..14198) /locus_tag="10S15_21" /note="Predicted by MGA" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72614.1" /translation="MELKKEFFQEADKAISEFDSIYDFFKVAKSHNAYQDGARYEKYK KENRMPSSVIIAKFVGFVETDLLYECMKESLDKVGPGRSSEDLVERFYRDNHNYKRNE ERKRERRLKRKLEALDLILKMEGWD" gene complement(14285..14560) /locus_tag="10S15_22" CDS complement(14285..14560) /locus_tag="10S15_22" /note="Predicted by MGA" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72615.1" /translation="MTNNDKMIRANFAFILFILIAVCVNLNSRVRVLETSNSDLQQTI QTQKDELEKIKEKNTMQDVIINKLNNDYNSRMAWQLQEVADENEVGG" ORIGIN 1 ttctttttta ttcttccttt tctttatcct tgcgttcgtg tctgtctttg gatttctcta 61 ccattctctc gtgggcagct tctatctcag ccgaagcttt ggcaatctca atctgctgcc 121 ggttcgcgtc cgtggtcaga ttgattgtgc ccgtggcctt gtcgtactgg atattcaagc 181 cctcgatacg tgagttgagg gtttctgctg ccgacgcaag ctctttcttc tgagcggcag 241 tcttgttttc aaccgcgttc agttcgtcga tcttcttgac taatcgttca ttgtcctcgg 301 ctgtagcttg gatctcattt ctgcgatctt tataggcttc attgcctttg ttgatacttt 361 cgtgaagatc atcgagggat tgctggaatt cttttgcttt ctctttcgcc tttttggttt 421 cttcgctttc ttgcgtcaac catgacacca gaccagcgat agcaccgaca acgataaaga 481 ctccaccaga tgatagagct gccaaagccc cagcgagtcc agtagtagca cccgtggcca 541 ccagtgaagt gctggttaag gatacaaggg aagtgataag cgtaccaatt aggctaccga 601 ttcccttgat aatggatagc cccagcatag ctcctttaaa gagcaagact gccgatacga 661 caccagcgaa caccgagata agcgggtcta aaacaggttt gaggaatcct agtacactta 721 ctagtgattt aacaactgga gtcgcaccac gaatgacact aataatgaca ttaaaagtac 781 tatttactgc tcctttaatg ctatcaagat tctgagcaat actcttacca gtaactgcct 841 tactcatctt gtcgaactcg gcaataacgt ttgcgatccc ttttgctacc gcgttcacga 901 tgttaccgaa tgatgtttta ataccttcag agtttttctt cgccatttca gcgaagccgt 961 ttgtaccctt gttaagctca atcagtcgct tactgaaatc agtaaacgtg atctttccgt 1021 cttgtaaggc tgaatagaag tccttttgag ccgatgcacc agcaaaacca aaactttcag 1081 cggtcttctg caaagcataa ggcatagttt cttgcaaggt tttccaactt tgcatatcga 1141 ccttaccggc tgataacatc tgagtgtact gttgcaatcc acggcttgca tcttctgtag 1201 atgcaccaga agcaaggaac gcattgttca aagcgattgt tagcttagta gactgcttca 1261 agttaccagt cattgaggtt agtttttgag tggtacttac aactgtatca agtgttgttg 1321 gtaagccctc gataccctca gaaagcaact tagtcgaggc tgccacatct tttgaagagt 1381 gccccagcga cttcatgact ttagggaatc gttgcaaggt atcgaaccgg tcaatagctt 1441 tatccattga ctggctgaca aggttcattg cagagctgac agccttaaag gctaccgcac 1501 cgaccgagaa gttcttgatt gcgtctttga ttttctcaaa gcctttagca ccttgcccgg 1561 ctttgtcacc accagccttg gcatcttcac cagctttttt aaacccagca ccgcccccct 1621 tggcttcctc gccagaggct tttaccttat gtccggcttg tttaaaacct tcaccgccag 1681 aactagcttc attgctagct gacttgattt tgtttgatgc ctgtttaaaa ccgtctccag 1741 atttttgggc aagatcagaa ctttctttta ctttctgacc ggcttgtttg aagccatcac 1801 cagaacgacc agctagatca gagctttcct tgatcttctc tcctgcacgt ttaaaaccat 1861 ttccagattt tgaagcgact tctgagcttt ctttgatctt atccccagca cgacgaaagc 1921 cgttacttga ggtttcggat agcttcgcac cctcggccat acgatcacca gcgcgtttaa 1981 aaccttgtcc agctcgcaga gccttgtcac cagtagattg gataccatcg cctgcagttt 2041 tgacaccttg gcccgatctg cgggcttcag actccaaacg cttcaaggca tctgatagct 2101 ctgtaagttt gtgaccattt acctggacgt caattacaat ttttccatct gccatttatt 2161 catctccctc ctttccgtct aatctgtatt tattttgtag ccggcgcatt ttggccttgt 2221 actcgctact atcgttcttc gagggtttcc aagaccgtat ctctactaat tgagatacag 2281 ccgtcccctc tggcataccg ttcaatagcg cgataaattc gggccatgtt agccggcctt 2341 gtgcttcaaa gaggttgata ttgtacgctt gcacaaagct agcgtatatt tcctgagcgt 2401 ctacttcaaa atcaatcaaa cggatatctt cttcttcgtc cttggctatt ggcatagggt 2461 tgccgtgccg gtcataaacc acgcgctctt ttttagtctt taaaaaatgc tcgtcgatat 2521 attcccacac ggctactata tcctctggat tgtccaaggc ttcgtccgtc atcattaaaa 2581 ccgctgtacg catcttctcg agattattca taacctcatt gtcaaacatc tcgaatacgt 2641 ccagcaccag atcgaaggaa cagtccacct cataggtgcg cccgttaact tcaaaggagt 2701 tttctaaagg ctcatttaac ttcatgagca atcccccttt ttactttttg ctggttttct 2761 ttgttttctt cgcttttgct ttctttacaa atgattcagc aaccgcaccc gatgccttag 2821 cccgctcttg gcctagacgg tcaagttcag ctcccagcat ggtatctacc tcatcaaatg 2881 catgatccaa agcgtcaagg tctggataac gttcatagag tttggcaaag gtaccgtccc 2941 cgaatagcac atcatactta atctccgtca tcttcttctg catttcaaag gcttcgtcaa 3001 taacttgctt gttaataact ccctctttaa gatcgtcaaa ctctccatta ttagaccgct 3061 caataagctc taactggtat ttattaaagc gttctgcgat ttcttcctgg agcgttgcaa 3121 gccgtgagat attctctagt gatgtatcaa attgtagttc gatttctccg atattgatag 3181 ggatatagtt gcgttttaat ttgattgaaa tagacatgtt tttcctcctt taatgcacaa 3241 aaaagagcgt cccaaaatgg aacgctttta cttttactta ttagcccacg actgctgttg 3301 ttttaggaag tgagttgtaa gagatcttac aagaaaattc ctcgtagttt gcagcggcac 3361 cagagcctgc cttgattgct gacacggtag caattccgac gtgttggttt ttaccgtccg 3421 aatcaactac tttatgccaa acaaggcgat cgttaccgag tttgtacttc aacccagcga 3481 taagagccat tgcttcatct tctttgtcgt aggtaccttt aaatgtgtat gatcctttta 3541 cagatgtaac tgtagtttct tccgtaccgt caccgtcgta ataagcgact gatgttgtag 3601 cttcatctgt atcgtcgtcc acatcttcga tccattttgc aagctctttg taggcttctt 3661 ttgctggttc agtctttgga tcagtgactg gtgcgataaa atgcccgcgt agggcgttct 3721 tttgacgtgc catgtattac actcctttgt tattcaagat tgttaggttt gcagttatat 3781 cctgcagata aatataaaaa ccctgctcgt cccgttcatt taaggacggc tgggtagtag 3841 ttaagttatt aaaaatatat gagttgtttt gacttggcaa gactagatca aactcagaaa 3901 gtgccttgtt aatttcccaa agacactcgc tagctgttac ctggttctta accttgactg 3961 cgatttcaaa aataaggctc acatctcgcg acccgtccat gtaaacacgc tcaaccttac 4021 cgcctggcaa cggataaagg accaaagagt ccagctcgct cagaaagtca agctcacaag 4081 caagcggtag accgagggtg ttgataaaat cgcgcaaaac aacgttaaaa tcattgttac 4141 ttttcattta ttaaacccca ttgctctcaa tccgacttct gcccacttgt taccgtggtt 4201 agctgaggcc tttaagtccc agcgcttacc agttccaggg gtggtatact tgctaaagct 4261 aaaactacgg tacttgttat aagcaccacc gtaaaactga gcgcgtgcgt atggtgtgtt 4321 atagataatc ttagagccgt cgcctgccac atgaccgcta gtgcgtagcg ggtcattcaa 4381 taacggaaca tacggttcca tatctgataa gatttggtca gcaatctcta accttgcttt 4441 acgttctgat gcttgcgata ctttcttagt agctccactc aaatcaatcg tgacattgat 4501 gcccatcaca tcacctcgat ttcgtagcaa aagactttgc ggttgaatgg ttcataaaca 4561 ggaatgatct tgtttacgat gtattcatcg tcgccatctt ttataatcga attgcgaaat 4621 gacgaatcaa gttcaacgtt gcaatagcga gggtatacga agataacacc aggcgctcta 4681 aatgacgggt tcttttgtcc ggacgggtta ttgactgacc cagggccgtc aaagttacgg 4741 tcgaagcgta ctgggcttaa aagaataggg taggagaatt cttctttccc ccacccgtct 4801 tttttgcccg taggttttga gatcgtcacc gaatcgacaa gtgtccgttt atcaacaacg 4861 accataatcc accccgctat acagaaaacc agccgattta agagtgttaa aagcatcaag 4921 ggatagatta tacccggacg ctgtttcaga cgctctagag ccgttatttg agccatagga 4981 caccgatgta cgtcctagtg tggtacttga tattgtctgc ttatcctcgg ctgttaaaat 5041 gcccgtgctg tccaggtact ggatctggta agcaacggca agtttaacgg cctttttacg 5101 aatcttatga tctttgtcaa aatcatggaa ctcgtaaaag tgacggataa agaggtcaat 5161 agcaagctct gctcgtttta gtagctcatc aaattcgcat gtactctcaa aacctaactc 5221 acgatattct tcatgcgtta agtatgccat gatacctcct attcagaggc cacctcttgg 5281 gccacgggtt cgctgtcaga aacaagaacc aaccactcct caccaaacgc gaggtttgtt 5341 ttttggttga tttcttctgc ttctgcagtc gtcaactcgt agaccttgcc ctcgtcaaag 5401 ttttggtctg ttgactcgat caaaaagtta caagtagctt tatattttgc cattcgttac 5461 tccttgatct cgtacccact agtcagaaaa gcagatacta gattgggatc agtgatggta 5521 aaggttacat cgtcctttac caagactgtc gcaacctgtt cagtcactgc ttctgtttta 5581 gttgtttttg tttcttctgc cattcgtcac ctcttacgca gttttatgca cgtagatcgc 5641 tttcttcttg ctgtcaagga caaaggcatc gtaacggata cgaccttcta caaggtagcc 5701 gttgatgcct ggtgggttat cgtggatctt gtagtcttcc aatttgacag gagaagtagt 5761 tgcgataggg tgcgcaataa caaacgctac gttttctggc aagcgagaag taggtgtcaa 5821 gataacaggc aagccgtcga taactcccac ttgaccctta aacgctactt cttgaccgag 5881 gtcagagttc ttcacaaatg atggatcaag tttgatgagt ttataaaact caggagatac 5941 gtggagcttg cgtccttctt ctggtacaag cgcatcagtc aatttaactt gaccgtcaag 6001 cactgcttca taagcgttgt ttttagttac tgcgccagtt ttaacgtggt cagtgtcagc 6061 accagcaacg actttaccaa agcggtactt atcaacttct ggaatgacaa cttctgaaag 6121 ttgacgggca agagccttac ctgcttccaa agttccgttg gtatcttgca ctgaacgttt 6181 gtcgatcgtg aacgtgaatg aacggtcttt tgtaagtgtc aatgtttgta cattgttttc 6241 cagttcggct gccgtaccgt aacgagtgtt accggtaagt gcgtagtcgt tcattgctgt 6301 tgttggaatt gagtatacct taacggtatc cacaccagtg aaatcaaaat cctggttaat 6361 aataccagtc gagagggctt ctttggcaaa gcgctcatct acttttgcgt caaatttaga 6421 tgcatagtta atagtcatat aggcttatcc tactttcttt tatttttaaa tgctgtcaaa 6481 gccagcaaat agagcttgtt cttccgggct gaggtcgcta tcaccaccag cagacggatt 6541 gccaccaagc gcgaactttg gctgtggttc ctgtggttct tccttttgga taaaaaggta 6601 agggcttgat tcctttagac cgctgatagt ttcttctagt tttggcttgc catcttctgc 6661 aagttcgatc ttgtcaagat caataaactt cataaggtct tcggagttat gagctcccac 6721 gtctttcaaa gctaaagcaa ccgcgttggt tttcttaact tgagcaagat tcgcttcact 6781 atctgtcttg tagctttcaa attgagcttg taagtcttcc agttgtttct tggcttcttc 6841 actagctccc tctttagctt gtaagtcctt gatagcttgg tcccgttgtt caagttgtgt 6901 ttttaagctg tcattttctg cttgtacttc agatctggcc tctttgattg ctgacccgta 6961 cgctgccata atgcgctcaa tagtgtcctt gtcttcgata cctgcatcaa ctaacatctc 7021 acgttttaaa ctcatgttta aaactccttt ctgttttacg tccagaagac gaattcgccg 7081 gtttacgtcc gacaacgaaa gcgcccagcg ggtaacgatc ccgcgaatag gtaagaaaaa 7141 aggaggaaat cacctatcca tccagaaagg gcgcaaaata aaaaggctat aaaagccttt 7201 atttttccat tccacttaaa aatccttcaa aagcggattt tgatagttct ttctgataat 7261 ccaaaaaggt ctttttcttt ctaaacaaaa ccttttctaa taatcgaata accttattca 7321 tcattagcgc ctcctttcgg tttaaaatat ctttccctcg catagtcacg atgtaagaaa 7381 ggcttatccg cgatataatc tcgcaaggtt gcttgctggt ctctgatttt ggttttaaac 7441 ttactgataa gttcctggtc gccaagcttc tcggcaacgt gtaacttctc tttagacttc 7501 ctaatagctc gctcgtatgc cctttgttta gattgggcat tagcatttct aatagcttct 7561 tcctgcgtta tattcttaac gtctgggcct agttctggta gttcgtttat accaggtaca 7621 aaaggagtaa gcatgtgtcc gcaattgata ccaagacagc ccccagccgt cccgtagccg 7681 tgatctgcaa gagatagaat actaataccg tgttcttccc tcgctgggcc ataggtaact 7741 atatggtgct gtaagggtgc gcaggcttcg cgggccgttg ctttcttgga ataataaaag 7801 gtatcaattc ccagctcgtc cgctggcatc gtccgcatct ctcggtagct acgcatgaca 7861 gtagttttaa taaccgttct agcgtagttg tccactttcc aataatgccc accacgatca 7921 ataaaaccct taaagcctat ctcttgccat ttcatcacgg tttgagatac agccttatca 7981 tgcgtgacta gaccgaccac ttgacgggct acaacttcct ggaccatttg acgatagaca 8041 tctgtaacaa tgcctggaag cgtggtatta atcaagttac tgatgtcacc gtgcgactgt 8101 tcgaagtaac cggtcagcaa ctcctgcgcg tgcttagaat tgccaaaatc accacctcct 8161 aggtcgtcta tgagctgttc tttggtcgtc tgatagattt taaagccctc gtcttcaatg 8221 accttacgga gctgttcacg gcctatttta gagtagcggg cgattgtgtc caggttctgc 8281 tcatttagca tgtgcatctg gctcattcgt tctaactgcc agatatacgg gttatctgcc 8341 aacgattcag caccgcgctc taacaaccta tcaataacct catcgaatag gtcacgcgcc 8401 atttgatgat agatatcacc aacctgcgaa gcgcgtagct caagttgctc ctcgttaaat 8461 aataccgggt acttgttacg cgccatctac tcactctcca taaatatcaa cttcgctggt 8521 gctacgctct agctccatgc tttctgctgt ttcctttttg atgtccgcaa gcatttgtgc 8581 agcttccttg tctgacaagc ccagcgcctt agatatagcg tattgcttgc tgacaagtcc 8641 acttaataga gctttcgcgt agtagtccag ctcgttgttt ttatcaacaa agacaccatc 8701 atcaagattt accgtgatgt cgtccatctc agggatagga ccgctataca agctgtacag 8761 cttcccaatc tcacaaatag agatcacaag ctctttgata gactggtcta caaggcttac 8821 gatactgttt cttagctggt atgtatcaga gttttcagat acaacctcgg tcgcagtctt 8881 cattgactta ccgtcaaacg taaacattcc aggcgatact ccaacctgca tttcaaacaa 8941 ggcaaggccc tcgttgattg ccttgatata gtcgtctgac cggataggag ttgtaaggtc 9001 tgtgatattg atcggtgtat ctttgccacc gtctatctgt tcgtaaacgt tttgttctgg 9061 atcaaattcg cgcgtgacta aatcagtgtc tccgtgatgg tcaaaaccaa tccggacagt 9121 ttggtctggt actaatacgc gtcgttggcc cattcgtacc tcccacttaa attcatcata 9181 agtggtgttg ataaagtcaa tagtactttt agcattatca aagatagaca gacccaaagg 9241 gctgttgata tccttgttat tcatgcctgg tggttttagg taagtaaaca acggtcgtgt 9301 aagcccgtca agcgttactt cttcctcaag gtcctcgtat acctcagata gtggtacacg 9361 gtcaccaacg cgctcttttt cattcgagcg atacagctcg ttagtgattg tgtatttgcc 9421 gtccttggtc cactcgtgca actcgattag agtatagtag attgtttcct tgcttacagt 9481 cttttgactc ttattgatga tggctgcaga agatacgtcc tgtgtattag attgtagcgg 9541 gtaaaataca ggggcttgta caaatgaaat cttgatctta tcatcgtcaa cgtatggacg 9601 catagcaaga ccgcccaagg ccaaacagct ctcaaggtaa cgctcaaagt tcttgttaaa 9661 ccggtcattc agtaaaaccg tttgaatgaa ctcgtttgtc gttccattcg caacgcttat 9721 ttctgcttgc tcattaaata ccagactagc aatcttctta caagctgtac gtgcaatagg 9781 caagtggttt cgtgcccgct gtttatcaac tcgatttgaa ttgcggtagc ggatagggtc 9841 ccacttactc tgatagtatt tcaagttctt ctgaatacga ttgtactcgt cgatgttaat 9901 tgcgatttta ggatgttctg tgatattgcc tagtgattgg cttgtcatta catatttacc 9961 cctcttaaat atatttctta gtgattgtaa gatactcatt tcaaaccttt ctctaggctt 10021 ttaatcgtag tagttgtgca ttatcaacga ccatatactg gaacgcgtcg caagtgtgat 10081 cgtcctcttt aataactttc gggtcgtcgt ctttaacagt tttctcgtcc cattgataac 10141 gcttgtgttc ctcaatgaaa tacttgaggt tgttctcagt tgggaaataa taaaaacggc 10201 cattcgcaag gagcgactgg acgtactctg tcattattat tttcttcttc ttcgccactg 10261 ggtgccagcg aataccaaag tcttctaaat actgatttct caatgctccc tcagcactat 10321 ctatcgtcat ttcaatgact ggtacattcg gatatttctg cgtttgcttg atgacaaaat 10381 catggagctc tttagacaat acgctcggag ctttcttttt gactttgcct gctggactgt 10441 agtagtagtt atccacaaga tagagattag atctgttagt aaccacggca tgcaagcaag 10501 tagttgctga ctgttggtgc cctgtatccg ctgcgaataa ctgacctatg acgcgctcgc 10561 cgtctggtat cttgtctacg cgtttaaaca gatccatgtt atacacgttc gtaccaaggc 10621 caaccggctc acccagatat aaatacctgt agtagtcgta gtcgttggtc ttgatacgct 10681 caatctcgtc cagcatttgt tctgtaacaa agcccagttc gtcgtccaga tagctagact 10741 catgtatcaa atacttctca gccgttcgca acgaatcaac ccactcattg atccaattgt 10801 aagggttacg cggtgggttg tacgaccaaa agaactgtac aaacggatag tctgggtgct 10861 tctgacgcat gaatgtactg tttgactggt cgaactcttc cgaatcagca aactctgccg 10921 cttcctcgta ccaaacagca ataacctttc cgacctcatt agatttcagt ttttggaagt 10981 cgtcttgacc gtagaagtgg aacgtcgaac ccgtccgtct atgtacgatc ttataagggc 11041 ttttagtccg cttgaactga ttagccatgc caaacttatc gagcgcccag attatctttt 11101 tatacacgct gtcaaagatc gtgttaccga ctttacgtac aatgataatc tctacgcatt 11161 gtcccagcgt tatcgcttta atcatcataa aaacaagcaa gagagcaatg accgatgact 11221 taaacgagtt acgaccaccc tttaaaatat tataaggctt tgaagatctc cacactttgt 11281 aaaacttagg gttgatctct ttagttagtt ttatagttgg tttagtcgtt cgggatatca 11341 tcaatgatga ggattgactc atcagcacca cctcccgctt cgtctaacgc ttgggcttta 11401 cgtttgtttt caagctcaag agacttgata cgtgctttct gttctttctt atcaagcgta 11461 tccttggcac cctcgctgtt tactatctta gagatcaact ccatgtgacg tgagctacct 11521 tttaaggctt tttgcattgc taccaaaagg agagctgact cgtaatcgtc ctcaaaaccc 11581 atgtcctgta gtactctaga gagctgagga ttcgaaactt tggaaacaag tagagcctca 11641 agcgtctttt tcatgttcgc tttttttcgt cttgtttttc ccgaagcgat accgcctttt 11701 tttcctagct ctctcgtttc gctcacgctt cgttcattaa acgggattag gttttcttca 11761 ttagccatcg cctcacttcc ttttcaaata gtatccttag ttcaatttga cagccgtctg 11821 gcccgtgtgt tcttcccagc gttgaatcgt agcgtccaca taccgtggat ccagctccat 11881 accgaaataa gtacggccta aatcttcgca aacaatcata gttgagccag agccattaaa 11941 gctgtctagt acattccagc ctttcttgct ggagtttcgc actagccgtt caatcaattt 12001 caagggcttc atagtagggt gcaagccatt tcttgttggt ttattctcgt tgatgatagt 12061 ggtcggtgag ttagcctgca tacgctcgat atactcaatt agctcggcct tcgtcatttt 12121 attcaactct tccacatctt caatgatagt agtcaaagaa cggtcattca caaagtaatg 12181 tgctgccccc tctttccaac catacaagca aggttcatgc ttccactgat aatcttgacg 12241 acccaaaaca agggtgttct tattccagat taaacattgt cgtactgccc aaccaatgtc 12301 agagcaggca cctctaaaat tgtaaccctc tgaatctgcg tgccagatgt aaaacgcccc 12361 ccccttcacg tagaacggta tctgcagcaa agaacgcatc tctcaagaat tttctaaacg 12421 attcattatc catgctgtca ttcataatcg tgagagcttc ctctgtgcca ccttcgtagg 12481 ctacgttata aggtgggtcg gttacaatca agtcaatctg ttgtccgtct accaattttg 12541 caagctggtc tgggtcagta gaatcaccac acataagacg atgacgtccg agctgataga 12601 tatcgcccag cttcgcgatt ggctctttag tttcttctac ttcgaaatca tcctcttcaa 12661 cttcggactc tggctcgtag tagtcaaacc cgaaatcttc catgttgatg ttcacaatac 12721 tttcaagctc tgagttcaaa agttcaatgt caaacccaga gttcatagtc aacttattgt 12781 gagcgaggat gtaagctcgt ttctgctcat catccatgtg agatagacga ataacttcaa 12841 cttcatcata gcctaactct tgcaaggcct tatagcgtcc gtggccctca ataataacat 12901 tgttttcatc aattgctatg gggtcattat tcccaaactc ctggattgat ttcttgatct 12961 gctcaatttg ttcgcgaggg tgtaacttcg cattgttctc atattcagtt atctctgaaa 13021 tattgatctt ttctattttc atttttactc caagcaccaa aaagagcgca ccttgacaat 13081 gcgcgcttct cgggttatat ggtctacttt gtcctcattg acaaaatatt tcaaagggcc 13141 tagtaagtag caccaaactt acatcatcgg tcactttcat tttttgtttt tttgcggtgc 13201 tttttatagc cggggcagga atcgaaccta cattatacgg gtgaaaagtc cgttactcta 13261 accgttgagt tacccagcaa cctactataa ggagacaacc aaatggcgca ggtccttcct 13321 acttcattgg ataatactat aataccactc aatacagcgc ttttactgtc aattttcttt 13381 caattatctc ccaagaatct gtattctagc aattccccag ccttatacgc ttctgcaaac 13441 tctaacaatg cccggtccag caatctatag tattcacttt ctgaatatcc cagacttgga 13501 tatatagcct tgtcttgtct aaatctcact ctacaatatc gctcaatcaa aatctgtgat 13561 agattgagat cagacagccg gttaatagcc gatgccataa gctccagttc ttgctgggcg 13621 cttacccgtc taatcaccat ctgctcagtt tgacggctgg gagagcttgg tgcgctcttt 13681 ggctctagtg agtaagtagc tgtgacttta gggctgtatt cctccccagc aattcttaac 13741 agtacgcggt agttcttgag cgtattgtct gcattctcct ttgtcttatt ttttagcact 13801 tcaccaaaaa gcatttaatc ccacccttcc atttttaaga ttaaatctaa cgcttctaat 13861 ttacgcttta aacggcgctc tcgcttgcgt tcttcgtttc gcttataatt atgattatct 13921 ctatagaatc gttccaccag gtcctcgcta gaccgtcctg ggcctacttt atcaagtgac 13981 tctttcatgc actcgtaaag cagatcagtc tcgacgaatc ctacaaactt cgcgataatt 14041 acagatgatg gcattctatt ctctttctta tatttttcat aacgcgctcc gtcctggtaa 14101 gcattatgac ttttcgcgac tttaaaaaaa tcatagatag agtcaaattc agatatagcc 14161 ttgtcagctt cctggaaaaa ttcttttttc aattccatcg tcttccccag tcttaatcgt 14221 gattagctca gcacttctca tgatattatc cataatcatc tgacacaatt cttctggtgt 14281 cagattatcc tccgacttcg ttctcatctg ctacctcttg tagttgccaa gccatacgtg 14341 aattatagtc attgtttaat ttgttaatta tcacgtcttg cattgtgttt ttttctttga 14401 ttttctcgag ctcgtccttt tgcgtttgga ttgtttgctg tagatcgctg ttgcttgtct 14461 caagcacccg tactcgtgag ttaaggttga cgcatacggc aattaggata aaaaggataa 14521 acgcaaaatt cgcacgtatc atcttatcat tattcgtcat tgcttgccct ttctaatatc 14581 agtagttgtc agtttactcg cgttcatctt gccgacattt atatttaggt ttccgttaat 14641 ttttaacgtt gttttatcgt tggtttttct gcttctaaaa gctatcacac tggcccagat 14701 caaaccagat agccagacca gtgcgagcag taggtatata aagtattgta gggtcattgt 14761 ttcgtttccc ccgtcattct atttctttca actcttaatt taaaactagt attatcaccg 14821 aaatatacta gagttgtttc ttcctcccac tgactttttg tgtatgggta tctgtttggt 14881 cgtgtcatgt ttactcctca ttcatttctt taagggtatc ccacatacct ttatgtaggt 14941 ttgtgatatt t //