LOCUS MF417962 14890 bp DNA linear ENV 27-FEB-2018 DEFINITION Uncultured Caudovirales phage clone 2AX_7, partial genome. ACCESSION MF417962 VERSION MF417962.1 KEYWORDS ENV. SOURCE uncultured Caudovirales phage ORGANISM uncultured Caudovirales phage Viruses; dsDNA viruses, no RNA stage; Caudovirales; environmental samples. REFERENCE 1 (bases 1 to 14890) AUTHORS van Zyl,L.J., Abrahams,Y., Stander,E.A., Kirby,B.M., Clavaud,C., Farcet,C., Breton,L. and Trindade,M.I. TITLE Novel phages from South African skin metaviromes JOURNAL Unpublished REFERENCE 2 (bases 1 to 14890) AUTHORS van Zyl,L.J., Abrahams,Y., Stander,E.A., Kirby,B.M., Clavaud,C., Farcet,C., Breton,L. and Trindade,M.I. TITLE Direct Submission JOURNAL Submitted (29-JUN-2017) Biotechnology, University of the Western Cape, IMBM, C2, Life Science Building, Robert Sobukwe Rd, Cape Town, Western Cape 7550, South Africa FEATURES Location/Qualifiers source 1..14890 /organism="uncultured Caudovirales phage" /mol_type="genomic DNA" /isolation_source="healthy skin from underarm of human male" /db_xref="taxon:2100421" /clone="2AX_7" /environmental_sample gene 440..1222 /locus_tag="2AX7_2" CDS 440..1222 /locus_tag="2AX7_2" /note="Similar to YP_164271.1 [Pseudomonas phage F116] (BLASTp score : 140). Similar to PB000740 [Pfam-B_740] (hmmscan score : 109.6)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72616.1" /translation="MVRAILEGRKTVTRRALNAQALKNIGYGVQLGECHELPSEGPLH PNSIGYYIDFCPFGQLGDRLWVRETWQDVHPVQVIDRYSQLGRAGIPGPPGVTYHTIY RADGEYPKVHYTHEHPYRCIEPDPNHGFLGAADSGWTGWTPSIHMPRWASRILLEITD VRVERLQDITEAQAKAEGCFFTDYGQACFHGGRGIRDATECEYPASKHQQRPGWMWDK TNSYEQCLDSARHAFGNLWNSVGGDWEDNPWVWVVEFKRVTP" gene complement(1514..1762) /locus_tag="2AX7_3" CDS complement(1514..1762) /locus_tag="2AX7_3" /note="Predicted by MGA" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72617.1" /translation="MDVSHNFDAPIAHAYRGHVMFLKFDWRRPNDQSPVAAKIIEPAP INGLGEVAAELLGPWPDYPAALDDAMAAAERWIDSQLP" gene 1891..2100 /locus_tag="2AX7_4" CDS 1891..2100 /locus_tag="2AX7_4" /note="Predicted by MGA" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72618.1" /translation="MKLTLILLGALLYLVGTLGWFGWFAIDLLDTGTTEALLYAFAGT CAWLLISFGLAIHIIKTARPTVGGR" gene 2103..2336 /locus_tag="2AX7_5" CDS 2103..2336 /locus_tag="2AX7_5" /note="Predicted by MGA" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72619.1" /translation="MAKVLAQITVKLPRLMEAGEYRKLRYVGGKPSLQQLKKWIEEGE VIGEVKGGMYFVDVQAAVMGSSDPLLAKMLEIG" gene 2336..3472 /locus_tag="2AX7_6" CDS 2336..3472 /locus_tag="2AX7_6" /note="Similar to YP_007111602.1 [Enterobacteria phage mEp235] (BLASTp score : 106). Similar to PF00589.17 [Phage_integrase] (hmmscan score : 45.9)." /codon_start=1 /transl_table=11 /product="putative integrase" /protein_id="ASN72620.1" /translation="MAARPRTLQNRKLPPNLYPNGKYWRYRNPVTGVMTSINRPLEEA IKLARAANLKMAELVVDDGSLLAVLTGDRLPIVSNLLTRFEEDWLPHRSYAVRTLEEI KFKLERYRQDLGDRLIGQLDVLAMAEYLDNFSNNAYTKHRGLWVQIFAFAVAKGLAER NNAELTLVKKEAEKKRQRHTLDGLKVIINAATTPPWLKRAIRLALASLQRRDDIVTWL KSAADMEKNTLTVSPGKTQGYDNPVHLKITMGATLREVVGECLRSPLASPYLIHYKPK ARRREQIDAKDHWTSVTPDYLTKEFSKARDAAHAYDHVPAGERPTFHEIRALGAWLYE QQNFPQEYIQALMGHADEKMTKHYQEGHDEKKIEYVEVGAELAF" gene 3843..4202 /locus_tag="2AX7_7" CDS 3843..4202 /locus_tag="2AX7_7" /note="Predicted by MGA" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72621.1" /translation="MNVDMDTDDWLGCPTPLEMYQHQCSILVDELVETERMLRRARAN IAGLVQMNDLLMTGKDQAETSLKDALSQVAALNLETSRQGRKLNGLAIITEQRDHLLR ENQRLLLELSVYKQPLA" gene complement(4194..4403) /locus_tag="2AX7_8" CDS complement(4194..4403) /locus_tag="2AX7_8" /note="Predicted by MGA" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72622.1" /translation="MAINVDQVNAMEAWFALRNDPTFISATPEERYETRLALADDLKQ QGLINDGEWRELTEEAVAAYADELG" gene 4487..5167 /locus_tag="2AX7_9" CDS 4487..5167 /locus_tag="2AX7_9" /note="Similar to YP_164331.1 [Pseudomonas phage F116] (BLASTp score : 338). Similar to PF02586.9 [DUF159] (hmmscan score : 140.7)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72623.1" /translation="MCGRLSQYRGIHDFVAALSMPNALANSVGDQPIERYNVAPTTAV ALLHLKGDLLHADPVRWGWRPHWAKDRAAPINARVEKVAHGPFFRAIWPHRAITPIDN WFEWVDEGGPKKQPYLIRRRDGAPIYCAAIGQLPDADEGPGEHDGFVIITADSAGGMV DIHDRRPVVLTPDLAREWLDPATLKERAEQMVLHQGEPSDVFEWFKVSMAVGNVRNKG PELIRPLG" gene complement(5321..5836) /locus_tag="2AX7_10" CDS complement(5321..5836) /locus_tag="2AX7_10" /note="Similar to YP_006590002.1 [Burkholderia phage DC1] (BLASTp score : 61.2). Similar to PF03245.8 [Phage_lysis] (hmmscan score : 84.6)." /codon_start=1 /transl_table=11 /product="putative Rz" /protein_id="ASN72624.1" /translation="MTSVQKLAGLVVLILVLMASAAGVTWQVQDWRMGNKLAQQAVLH GDDLAAISNAATAQARAEQGKRLGLEQQLAGQDQQHTKELSDAQRNQARLRDQLATAD VRLSVLLAEDSASGCNVPTTPGTAGVVHAARRAQLDPAHSQRIIRITDDGDNAIIALR ACQAYVRAIAR" gene complement(5833..6258) /locus_tag="2AX7_11" CDS complement(5833..6258) /locus_tag="2AX7_11" /note="Similar to YP_007392728.1 [Pseudomonas phage JBD5] (BLASTp score : 140). Similar to PB000783 [Pfam-B_783] (hmmscan score : 177.4)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72625.1" /translation="MSITETRGVRNNNPGNIDYNPANQWQGQLKADPALEKRFARFDT PENGIRALGKLLLTYQRKHGLKTVKAIISRWAPSVENDTAAYVRAVEANTGTRPGAEI DLVNPVVMAGFVKAIIHHENAGYAYPDAVLAEGVRRALA" gene complement(6302..7303) /locus_tag="2AX7_12" CDS complement(6302..7303) /locus_tag="2AX7_12" /note="Similar to YP_004934057.1 [Escherichia phage HK639] (BLASTp score : 156)." /codon_start=1 /transl_table=11 /product="putative tail protein" /protein_id="ASN72626.1" /translation="MARQGINLGTAPTGQGGDTFRTASQKNNDNSSELYTALGAPANG QLPAALPVAKGGTGGTTQLTARQGLGLGAASTKSFGLQDGELIPAGVLSGMFSNVAPD AYQMDRPGEPGQQGAFYKFLNNGSSSGLSYSTLLRLPYNTGYEAQIFIPMGTSQLAFR TVTGAAGTFGPTCSVYHTGNTTRGSGGALSAASPILRIANVSASERRDLQEESFLPAG EWGVANDEARGVIVERLGVGEYRVTGSLGLALEGWRTHDPSSPDGGRMLGITDSHQEE DGTVIIRLFKQRWTLTDDGEMVPGRGAPMDVPLNSWIDVRLEMPKVDTPPPLRSTEE" gene complement(7332..7661) /locus_tag="2AX7_13" CDS complement(7332..7661) /locus_tag="2AX7_13" /note="Predicted by MGA" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72627.1" /translation="MWGADGKQIFDSAVQAAVFTRVAQNWTFTHAEQSGQGLITNWYS VPLNYALGDYLMINNARMPMMAGNNESRGTGLRYDFPAGLIRFSVTTVTNPTYFSLSA VFGKLAV" gene complement(8305..12138) /locus_tag="2AX7_14" CDS complement(8305..12138) /locus_tag="2AX7_14" /note="Similar to YP_007112295.1 [Enterobacteria phage HK633] (BLASTp score : 886). Similar to PF13550.1 [Phage-tail_3] (hmmscan score : 70.4)." /codon_start=1 /transl_table=11 /product="putative central tail fiber" /protein_id="ASN72628.1" /translation="MGAAQKLDIHGAKGGESKPKSPVEAPDSLRSTNVAKILIAVGEG EFDGTPTARDIFLDNTPIQDASGNFNFTNVKWDWRPGSVEQTYIPGIPSVDNETSLNI ELRSGTPWVQSLTNLQLSAARIRLATPRLASQDAEGNIGGYSIQYAVDVATDGGAYQE VLVGAITGKATTRYEKSMRIDLPPATSGWLIRVRRITPNQNTDKIADSLFIAGYTQVI DAKLRYPNTALLFIEFDAEQFTNIPAVTVKCKARRWQVPSNYDPVARTYSGAWDGTMK EAWTNNPAWITYGICTQDRFGLGRRIKPWMVDKWELYRIAQYCDQLVSNGADGVEPRF LCDMNLQGKADAWSLLRDIAGIYRGMTYWAQGQLVMQADMPRAQDIDYVFTRSNVIDG KISYGSASAKTRFTRCLVSYDNPLNNYDTDVTVYSDLPLQRRLGDKPTEISAIGCTRA SEAQRRAKWLVLSNNQDRTISFRTGMEGRIPLPGFIIPVADSLLAGREIGGRIAAAVG KVITLDRDTLAKAGDRLVINLPGGRAEGRTVESVSGRNVTVTVAYSEAPAAQLQWAID ADDLAIPLYRVMRTARTPEGDYDISALQYEPSKFPSIDTGARLEERPISVIPITVVPP PASVTVTSNVSIDQGLAISTMNISWPAVAGAVAYDVEWRKDSGNWIKVQRTGSTSVDV TGIYSGAYLARVRSVSAFEISSIWKSSTLTSLEGKTGLPPAVSSLTTKGELFGISIKW GFPAGAEDTQRTELWYGPANNLEAATKLADLAYPQADYRMQSLLSGATLFFWARLVDR TGNIGPFYPTVNGVMGQSVSDAWPLLEQVKGQIDESSLAPALNGRIDLIDGDGPGSVN ARNEQLRKDLEEQIKPYVDALLWDAAKAYVKGDIVRQGNKLYQALEANSGSQPPSENW KDVGDILTETNALAIRVDNLDQEITAVDGRVVATQEQLTQLQTKVNDPVTGLAATVNN VTQVNQRVTNVDNKLTAQTERIDGVYAEINAPMAGSENDLAGGTQGYAGVWSIQSAIT DGDYAQGKLTQTVEAQVIKNYATYEETTTALVNQQTATTSQVTALRSDFDGNKSTVQT QIKTVSDQASATASQTTQLQTTVNGHTQQFQQQASINQDVNGKITGTYSFRFKFDVNG APYSTGVGLGIETGANGQVTSRFVIQADQFAIYNGEVGAAGSSVPFAVNGTETYIKSA FIQDGTITNAKIGSYIQSNNYVAGQTGWKLFFDGTFEINGVAPGQGRSMMTNRSLRFW DANNVKRLQLGDQTE" gene complement(12196..12774) /locus_tag="2AX7_15" CDS complement(12196..12774) /locus_tag="2AX7_15" /note="Similar to YP_009004652.1 [Edwardsiella phage eiAU-183] (BLASTp score : 172). Similar to PF06805.7 [Lambda_tail_I] (hmmscan score : 42.2)." /codon_start=1 /transl_table=11 /product="putative tail assembly protein" /protein_id="ASN72629.1" /translation="MSAIVYSPMTTIKLSGSLAQKFGRLHRRQVGSGDTWEVFRALKA TIDGFEAEIRRLDRLGLRFAIFRNRKNTGPDQFGMGGTKEVRIVPVVEGSKRGGILQI VLGVVLIAASYFGAPTAPAGIALLAGGVIQMLSPQAAGLKQSASPENMPSYAFGSAKN TTASGNPVPICIGDRRWGGAIISASIYAEDKT" gene complement(12771..13526) /locus_tag="2AX7_16" CDS complement(12771..13526) /locus_tag="2AX7_16" /note="putative gp19; Similar to NP_037679.1 [Enterobacteria phage HK022] (BLASTp score : 234). Similar to PF14464.1 [Prok-JAB] (hmmscan score : 82.6)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72630.1" /translation="MRKHIIAAIQAHAAAEYPKECCGLVLAVGRAQKYFPCRNIATEP NEEFRLEPEDYAAAEDQGEVIGIVHSHPDATSRPSSRDLAMCEATALPWHILSWPEGD LRTITPTGSTPLLKRPFVHGAWDCWQVCADWYQREWGLEFEAFHRADGWWESADSTSL YEANYAAAGFEQVDSPKRGDMIVMEVGRTAHPNHAGIYLGTDPALPGEDSGVFGPGPF VLHHLYGRPSEVIVYGGPWLQRTRLILRHKEAR" gene complement(13530..14279) /locus_tag="2AX7_17" CDS complement(13530..14279) /locus_tag="2AX7_17" /note="Similar to YP_007111390.1 [Enterobacteria phage mEp237] (BLASTp score : 270). Similar to PF05100.7 [Phage_tail_L] (hmmscan score : 221.0)." /codon_start=1 /transl_table=11 /product="putative minor tail protein L" /protein_id="ASN72631.1" /translation="MSLINAIQTLEPGNEVMLFELDGSDYGADVLRFHGHAIPHTPAE LMAAGGNADQLPAKSIWWKGEEYGAWPMQYEGSEANGDGTAVRPKLSVGNVNGRITAL CLAFEDLLEFKLTIRSTLAEYLDAVNFEGGNPTADPTQESIEVWYVDQKTNEDGETVS WDLASPGDVGGETIGRQMTTLCHWCLTGGYRGPNCGYTGPYVTIDGIVTDNPELDVCD ATLGKGCIPRFGEGNPYPFGGFPAVSLIARS" gene complement(14290..14628) /locus_tag="2AX7_18" CDS complement(14290..14628) /locus_tag="2AX7_18" /note="Similar to YP_007151622.1 [Enterobacteria phage HK544] (BLASTp score : 112). Similar to PF05939.8 [Phage_min_tail] (hmmscan score : 131.0)." /codon_start=1 /transl_table=11 /product="putative minor tail protein" /protein_id="ASN72632.1" /translation="MAIETFTWATQHGEAPDITYRVRTSQFGDGYKQEVGGGINNKVD SYPVTHTGGQARALEIMAFFDRHQGAKAFLWTTPLGQLGLFTCKNPTPTPMGGGVFKV TATFERAFHP" gene complement(14631..14861) /locus_tag="2AX7_19" CDS complement(14631..14861) /locus_tag="2AX7_19" /note="Predicted by MGA" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72633.1" /translation="MLALGVGGGGGGGGGNTYNFPVSVSVQTAGEGGTATQEDTTQAG RNIQQATKAEAEAAIARGVQPGGAIWRAINGR" ORIGIN 1 ttgcggggtc ggtaccctct gcctgccgcg agtgggggtg gtgtggctgc gggtgcgggt 61 gtggactggt gagtggcgct gcgccgggcg tgccgggccg gtagcggtgg tgctgccggg 121 gcgcggcggt tccggcctgg gaagcccgac cgatggcttc gcgcggggct ggaacgcctg 181 cctcgacgaa gtagctcgcc tgaccgccac ggttcgaacc cctgagcccc tggttatgac 241 cgacggcctg cacacctacg agtacgtcac cggccacaac gccgcaatcg acaagatgct 301 gggcgtgaaa tcttgaagag tacggatgta ctccacccgc aaaacctgta acccctcccc 361 cttcaaagtc agccgctata gcggcaagga acccgccatg cctgaaataa aggaacggcc 421 aatactcttt tcggcaccga tggtgcgcgc catcctggaa ggccggaaga cggtcacgcg 481 ccgggcgctg aatgcgcaag cgttgaagaa catcggctac ggagttcagc ttggcgagtg 541 tcacgaactc ccgagcgaag ggccgctaca cccgaacagc atcggctact acatagactt 601 ctgccccttc ggacaactcg gggaccgtct gtgggtgcgc gagacttggc aagacgttca 661 tccggttcaa gttattgacc gctactcaca gcttggccgc gccgggatac ctggccctcc 721 aggcgtgacc taccacacaa tctatcgagc agatggggaa tatccgaaag tccactacac 781 acatgaacac ccctaccgct gcatcgaacc tgacccaaat cacggttttc tcggcgcagc 841 agattcaggg tggacaggct ggacccccag tatccatatg ccacgctggg ccagccgcat 901 cctgctggag atcaccgacg tgcgggtcga gcggttgcaa gacatcaccg aggcacaggc 961 caaggccgaa ggatgcttct tcaccgacta cggccaggcc tgcttccatg gtggacgggg 1021 gatacgcgat gcaaccgagt gcgaataccc cgccagcaaa caccaacagc gcccaggctg 1081 gatgtgggac aagacgaaca gctacgagca gtgccttgac tcagcccgcc acgctttcgg 1141 caacctatgg aactcggtcg gcggcgactg ggaggacaac ccgtgggtct gggtggtcga 1201 gttcaagcgg gtgacgccat gatcttgctc gcagcacctg cagccctctg gttcgcctac 1261 gtcttcatct acaaggggcc gaggccatga tcaccttgga gcaaattcgg gctgaggtgg 1321 ctgaccgaaa aaccgtagat caccacaccc gctgccgtga ttgcggccac ttcgtaccga 1381 aagagcgatg ggtgccgagg gaatccccac atgccgcacg cgggcagcgt ccactctgtg 1441 caccctgctt cgacgaatac gaaggcccac tctactaacc ccaatccccc tacatgcctg 1501 ccggtgagcg gagtcagggc aactggctgt cgatccatcg ttcagctgcc gccatcgcat 1561 catcaagtgc tgccggatag tcaggccaag ggcccaacaa ctctgcagca acctcaccca 1621 agccattgat aggtgctggc tcaatgatct ttgcggctac agggctctga tcgttcgggc 1681 gacgccagtc gaacttgaga aacatcacgt ggccccggta agcgtgcgct atcggagcat 1741 cgaagttgtg tgacacgtcc atgcctcatc acgaactaag ttgaaccctt ttgtacaccg 1801 cttcggtcct gtttgaaaga taggcagaaa gctatcactc caatccccta tatgccgcct 1861 cgcgtggcta ggacacaccc catgttcgct atgaaactca ccctgatact gctgggcgct 1921 ttgctgtacc tggtaggaac actcggctgg ttcggctggt tcgctataga cctgctggac 1981 accggcacca ccgaggcact gctctacgcc ttcgccggca cctgcgcctg gctgttgatc 2041 agcttcggcc tggcaatcca catcatcaag acagcgcggc ccacggtggg cgggaggtag 2101 ctatggcaaa agtcctggcg caaattacgg tcaagctgcc gcgcctcatg gaggccggcg 2161 aatacaggaa gttgcggtac gtcggtggaa agccgagcct gcagcagttg aaaaaatgga 2221 ttgaggaagg cgaagtgatt ggagaggtaa aaggcgggat gtatttcgtc gacgtgcagg 2281 cggcggttat ggggtctagt gacccgctgc tggccaagat gctggagatt gggtgatggc 2341 tgcccggccc cgtacgctgc aaaacagaaa gctgccgcca aacctctacc cgaacgggaa 2401 gtactggcgg taccgcaacc cggtcaccgg cgtgatgacc agcatcaacc gcccactgga 2461 ggaggcaatc aagctggccc gggcggccaa cctcaaaatg gccgagttgg tcgtagatga 2521 cggctcgctg ctggcggtgt tgaccggcga ccgcctaccg atagtgagca acctgctgac 2581 gcgtttcgaa gaagattggt tgccgcaccg ctcatacgcc gtacgcaccc tggaggagat 2641 caagttcaag ctcgagcggt accggcagga tctgggtgac cgcttgatag gccagttgga 2701 tgtgctggcc atggccgagt acctggataa cttcagcaac aacgcctaca cgaagcaccg 2761 cgggctgtgg gtgcagatat tcgcgttcgc agtggccaag ggcctggccg aacgcaacaa 2821 cgccgaactc accctggtga agaaggaggc agagaaaaag cgccagcgcc atacgctcga 2881 cgggttgaag gtgatcatca atgcagcaac aacgccgcca tggctaaagc gggcaatccg 2941 cctggccctg gccagccttc agcgccggga cgacatcgtt acctggctga agtctgctgc 3001 ggatatggaa aagaacacgc tcacagtgtc gccagggaaa acccagggtt acgacaaccc 3061 ggtacacctg aagatcacca tgggcgcaac gctgcgggag gtggtcggag agtgcctgcg 3121 ctcgccgctc gcctcgccct acctgatcca ctacaagccc aaggcccgcc ggcgagagca 3181 gatagacgcc aaggatcact ggacatcggt aacgccggac tacctgacca aggagttcag 3241 caaggccagg gacgcagcgc acgcctatga ccacgtgccg gccggagagc gccccacttt 3301 tcacgagatc cgcgcattgg gtgcctggct gtacgagcag cagaacttcc cacaggagta 3361 catccaggcg ctcatgggcc atgcggacga gaagatgacg aagcattatc aggaggggca 3421 cgacgaaaag aagatcgagt atgtcgaggt gggcgccgag ttggcgtttt gaggtggggg 3481 ttttgcaaaa gttttgcaaa agttttgcaa atcgcagaaa gcaaaaaggg gtcaccgttt 3541 ccgatgaccc ctctagaccg cccagcagag cggattttgt ttggtaggcg cgattggact 3601 cgaaccaacg acccccacca tgtcaaggtg accccggaac gctcgtaagt atttgatata 3661 tatagaaatc acgcctattc aggctaggcc aaaaacaagc ttatagccct ataagaatca 3721 ataacttagc gttggatatt gctacagtga ttcccttccc ggcgtcctgc cgacgaacac 3781 cactccccaa acccgcaacg cctcgattac tgtacacaca tacagtacat gagattcacg 3841 ctatgaacgt agacatggac accgatgatt ggctcggctg ccccactccg ctggagatgt 3901 accagcacca atgctcaatc ctcgtagacg agctggtgga gaccgagcgc atgctgcgtc 3961 gagcgcgggc gaatatcgcc ggcctggtgc agatgaatga tctgctgatg acaggaaagg 4021 atcaggcgga gacctccttg aaagacgccc tatcccaagt agctgccctg aacctggaaa 4081 cctcacgcca gggccgcaag ttaaacgggc ttgcgataat cacggaacag agggaccacc 4141 ttctcaggga aaaccagcga ctactgcttg agcttagcgt ttacaagcag ccattagcct 4201 agctcgtcgg cataggccgc aactgcttcc tcagtcagct ctcgccactc accatcgttg 4261 atcagcccct gttgcttgag gtcgtcagcc agggccagcc gcgtttcgta gcgctcctca 4321 ggcgtggccg atatgaaggt tggatcgtta cgcagcgcaa accacgcctc cattgcgttc 4381 acctgatcaa cgttgatcgc catgacgaat acctcggtcc agtgtctaca gtgtagagat 4441 tggccgggga ccggctgttc atcggcgccg acgagcggag atgcttatgt gcgggagact 4501 ttcgcagtac cggggaatcc acgatttcgt tgctgctctg agcatgccca atgctctggc 4561 gaactccgta ggcgatcagc cgattgagcg gtacaacgtg gcaccgacaa ccgcggttgc 4621 actgcttcac ctgaagggcg acctactcca cgccgatccg gttcgctggg gctggcggcc 4681 gcattgggca aaagaccggg cagcgccgat caatgcccgc gtggagaagg tagcccacgg 4741 cccgttcttc cgggcgatct ggccgcaccg ggcgatcacg cctatagaca actggtttga 4801 gtgggtggat gagggcgggc ccaagaagca gccctacctc atccgccggc gggatggtgc 4861 accaatatac tgcgctgcca ttggccaact gccggacgct gatgaaggac cgggcgagca 4921 tgacggcttc gtcatcatca ccgcggacag cgccggggga atggtggaca tccacgaccg 4981 gaggcccgtg gtgctgacgc ccgacctggc ccgggaatgg ttggacccgg ccacgctcaa 5041 ggagcgcgcc gagcaaatgg ttctgcacca gggcgagccg tccgacgtgt tcgaatggtt 5101 caaggtcagc atggccgtgg gtaacgtgcg gaataaggga ccagaattga tacgaccttt 5161 aggttaattg tcgtagaaat accacatgac agacgccact gtgacgaccc atccaagggt 5221 gagcagtatt gacagccccg caagccgctt atccatggtg cccttcagtt accaaaatga 5281 tcgaagttag agcgcacccg gcaatattag ctcaacgcac tcatcgtgca attgcgcgga 5341 cataggcctg gcaagcacgc aaagcgatta tggcgttatc cccgtcatcg gtgatgcgga 5401 taattctttg agaatgcgct gggtcaagtt gggctcgacg ggctgcatga accacgccgg 5461 cggtgccggg ggtggtaggc acgttgcagc cactggctga atcctcggca aggaggactg 5521 acagccggac atcagcagta gcaagctggt cacgcaggcg agcctggttg cgctgggcat 5581 cggataattc ctttgtatgt tgttggtcct ggccagcgag ctgctgctcc agacccaggc 5641 gcttgccctg ctcggcgcgg gcttgggcgg tggcggcatt actgatcgcg gcaaggtcat 5701 ccccgtgcag gacggcctgc tgggcgagct tattgcccat ccgccagtct tgaacctgcc 5761 aggtaacgcc cgcggcactg gccatcagca ccaggatcag caccaccagg ccggccagct 5821 tctgtaccga cgtcatgcca gcgcccgccg cacgccttct gccaacaccg catcagggta 5881 ggcatatccg gcgttctcgt gatggatgat ggctttgacg aagccagcca ttaccaccgg 5941 gttgaccagg tcaatctcgg cgccaggccg ggtgccggtg ttcgcttcga ccgcgcgcac 6001 gtacgccgca gtgtcgttct ctaccgaagg cgcccaccgg ctgatgatcg ccttcacagt 6061 tttcaggcca tgtttgcgct gataagtcag caggagcttg cccagggcgc ggataccatt 6121 ctccggcgtg tcgaaccttg cgaagcgctt ctctaaagca gggtctgcct tcagctggcc 6181 ctgccactgg ttggccgggt tgtaatcgat gttgccgggg ttgttgttgc gtaccccgcg 6241 agtttcggtg atcgacatgc ttttctccag gcgtaaaaaa gcccgctcaa ttggcgggct 6301 gctattcttc ggtcgatctg agtggcggcg gcgtatcgac cttgggcatt tcaagccgca 6361 catcgatcca gctattcagc gggacatcca ttggcgcgcc acggcccggc accatctctc 6421 cgtcatcggt gagcgtccag cgctgtttga ataggcgaat gatgacagtg ccatcctctt 6481 cctgatgact gtcggtgata ccaagcatcc ggccgccgtc tggtgagctt gggtcgtggg 6541 tccgccagcc ctccagcgcc aagccaagac ttcccgtcac ccgatactcg ccaacgccga 6601 ggcgctcaac aataacgccg cgtgcttcat cgttggccac accccattcg ccggcgggga 6661 gaaacgattc ctcttggagg tcgcggcgct cgctggcgga cacgttagcg attctcagga 6721 tcggcgatgc cgccgaaagc gcgccgcctg agccgcgtgt ggtgttgcca gtgtgataca 6781 cactgcatgt cgggccaaat gttccggctg ctcccgtgac agttctgaat gcgagctgac 6841 tggtgcccat agggataaaa atttgcgcct cgtagccggt gttgtatgga aggcgtaaca 6901 gtgttgagta gctcagccca gacgaagatc cgttattcaa aaacttgtag aaagcgcctt 6961 gttgcccggg ctcgccaggt ctgtccattt gataggcgtc cggtgcgaca ttggagaaca 7021 tcccactgag aacgcccgcc gggataagct ctccatcttg cagcccaaag cttttagttg 7081 aggcagcgcc tagccctaag ccttgccggg cagtcagctg cgtagtccca cccgtgccgc 7141 cttttgcaac gggcagcgcc gcaggcagct gtccgttagc cggagctcca agggcagtat 7201 aaagctcgct cgagttgtcg ttatttttct ggctggcagt acggaacgta tcgccgccct 7261 ggcctgtagg cgccgtacca agattaatgc cttgtcttgc cattgaatta aacccctata 7321 cgaaatgaaa attaaacagc cagcttccca aataccgcag agagcgaaaa gtatgttgga 7381 ttcgtcacgg tagtaacgct aaatctgata agcccggccg ggaagtcata tcgcagaccc 7441 gttcctctag attcattgtt tcccgccatc atgggcatcc ttgcattatt gatcatcaag 7501 taatcaccta gggcgtagtt aagaggaacc gaataccaat ttgttattag cccttgtccg 7561 ctctgctccg cgtgcgtaaa tgtccagttt tgcgcgaccc ttgtgaatac tgccgcctga 7621 acagcagagt caaatatctg ttttccatcc gcaccccaca ttcttaggcc atatgtggac 7681 aaaggcttgg atgaaaacgc agctacgaat atgtttccag agtgaacctg cccagaggta 7741 actgtggcgc cggtccacgc ccccgccgat ccttcaagaa gaacgccaag ctgaatcagc 7801 gagccattat ttggcggcct gataaaaaca aggggcggct cctgcgtgtc aaccacattc 7861 tgaaagacga cggacgcgcc agagttatag gtgcccttat gaaagacaca caacctcgca 7921 tattcagagt ctagggatat agcgccagaa tcattcacca cttgcagtcc gtaactcatc 7981 acgcccacct tgcaacaatg agacgcatgg ttgccgaaga gaatgaatcg gaaggacgag 8041 ctttgagaaa attcctaaca taaacttcgt tcgtgcccat ttcgcactcc aactgcctgt 8101 tagatacgac ctgcgagtta tcgttattga tcggtaacaa aaagcatatc gcgttgtttg 8161 tggtgcaccc tggcgctgca aaggtcctag tgcccagcgg tcctgaggaa taggacacaa 8221 gtaccgacag aacaatccta taagtggata tgcttgtatc gaactcaagg gtgccgttag 8281 gagaccaaat tcttaagccg gaactcattc tgtttggtct cctaattgca gtcttttgac 8341 gttgtttgcg tcccaaaagc gcagagatct gttcgtcatc attgatcgcc cttgcccagg 8401 agctacaccg ttgatttcga aggttccgtc gaaaaacaat ttccagcctg tctgccctgc 8461 gacgtagttg ttcgactgga tgtagctacc tatcttcgcg ttggttatcg tcccgtcctg 8521 gatgaatgcc gacttgatat aggtttctgt gccgttcacc gcgaatggca cgcttgaccc 8581 tgccgcccct acctcgccgt tgtagattgc aaactgatcc gcctggatga cgaagcgcga 8641 agtaacctga ccgttcgccc cagtctcaat gccaagtcct acgccggtag agtaaggcgc 8701 cccattcaca tcgaacttga atcggaacga gtaggtgccg gtgatctttc cgtttacgtc 8761 ttggttgatg cttgcttgtt gctggaactg ctgcgtgtgt ccgttgacgg ttgtttgcag 8821 ctgagtagtc tggcttgcgg tggctgacgc ctgatcggat accgtcttaa tctgcgtctg 8881 tacggtggac ttgttcccgt caaaatctga gcgaagcgcc gttacttggc tggttgtagc 8941 ggtctgctgg ttgacgagcg cggtcgttgt ctcctcatag gtggcgtagt ttttgataac 9001 ttgagcttcg acagtttgag tcagcttgcc ttgggcgtag tcaccatcgg taatagcaga 9061 ctggatcgac caaacgcccg catagccctg ggttccgcca gccaggtcat tctcagaacc 9121 agccataggc gcgttgattt cagcgtaaac gccatcaatt cgctccgtct gcgcggtcag 9181 cttgttatca acgttggtta ctcgctgatt cacttgggta acgttattta ccgtagccgc 9241 caggccggtt accgggtcat ttacctttgt ctggagctgg gtaagctgtt cctgagtggc 9301 aaccaccctc ccatcgactg cggtgatttc ctggtcaagg ttatcaaccc gtattgccaa 9361 agcgttcgtt tcagtgagga tgtcgccgac gtctttccag ttttcgctgg gaggctgcga 9421 gccactgtta gcctccaggg cttggtacag cttgtttccc tggcgaacga tgtcaccctt 9481 gacgtaagcc tttgctgcat cccacaacag cgcatcaacg tatggcttga tttgctcctc 9541 tagatccttt ctaagctgct catttcgagc gtttacggag ccaggcccat ctccgtcaat 9601 cagatcgatg cgaccgttaa gggctggagc caacgatgat tcatcaatct gccccttgac 9661 ctgctcaagg agcggccaag catccgaaac agactgtccc atcactccgt ttacggtcgg 9721 atagaaaggg ccaatgttcc cggtacgatc aaccaggcgc gcccagaaga acagcgtagc 9781 cccggataaa agcgactgca tacggtagtc ggcctgcgga tatgccaggt cggccagctt 9841 cgtcgccgcc tccaggttgt tcgcaggccc ataccacagc tcggtccgct gggtgtcctc 9901 ggcgccagca gggaagcccc acttgatgct gatcccgaac agctctcctt tagtggtaag 9961 ggacgaaact gccggcggca ggccggtctt gccttcaagg ctggtcaggg tagagctttt 10021 ccagatcgac gaaatttcga aggcgctcac cgaacgcaca cgtgcgagat aggcgcccga 10081 gtagatgccg gtgacgtcaa cgctcgtcga gccggtacgc tgcaccttga tccagttgcc 10141 gctgtcctta cgccactcca cgtcataggc gacggcgcca gcgacggctg gccacgagat 10201 gttcatagta ctgatggcca ggccctggtc gatcgatacg ttcgacgtaa cggtgacgct 10261 cgccggcggc ggtaccacag tgatcggtat cacgctgatt gggcgttctt ccagccgtgc 10321 tccggtgtcg atgctgggga acttgcttgg ctcgtactgc aaggcgctga tgtcgtaatc 10381 accctctggc gtccgggcag tcctcataac cctatataga gggattgcca agtcgtcagc 10441 gtcgattgcc cactgaagct gtgcagccgg agcctcgctg taagcaaccg tcacggttac 10501 attgcgaccg ctcacgcttt ccacggtgcg cccttctgct cgcccgccgg gaaggttgat 10561 caccagccgg tcgccggcct tggccaaggt gtcgcggtcc aaggtgatga ccttccccac 10621 cgccgctgcg atgcgcccgc cgatctcccg gcccgccagc agcgagtcgg cgacggggat 10681 gatgaaacca ggcaacggga tacgaccttc catgccggtc ctgaagctga tcgtgcggtc 10741 ctggttgttg ctcaggacta gccattttgc ccggcgctgt gcctcagatg cgcgagtaca 10801 gccgatggca ctgatctccg taggcttgtc acccaggcgg cgctggagtg gaaggtcgga 10861 atagaccgtg acgtcggtgt cgtagttgtt cagcgggttg tcatagctga ccagacagcg 10921 agtaaagcgg gtcttcgccg atgcgctgcc gtaggagatt ttcccgtcga tgacgttgga 10981 tcgggtgaag acgtagtcga tgtcttgcgc ccgaggcata tcagcctgca ttaccaactg 11041 gccctgcgcc cagtacgtca tgccgcggta aataccagcg atgtcgcgaa gcagagacca 11101 ggcgtcggcc ttgccctgca gattcatgtc gcacaggaag cgtggctcca cgccgtcggc 11161 gccgttggat accaattggt cgcagtattg cgcgatgcgg tacaactccc acttatcaac 11221 catccacggc ttgatacggc ggcccaggcc gaaacggtcc tgggtgcaga tcccgtaggt 11281 gatccaggcc gggttgttgg tccaggcctc tttcatggtg ccgtcccagg cgccggaata 11341 ggtgcgggcc actgggtcgt agttgctcgg cacctgccag cggcgggctt tgcacttcac 11401 ggtaacggcc gggatattgg tgaattgttc ggcgtcgaac tcgataaaga gcagtgcggt 11461 gttcgggtag cgcaactttg cgtcgataac ctgggtgtag ccagcgatga agaggctgtc 11521 cgcgattttg tcggtgttct ggtttggggt gatgcggcga acccgaatca gccagccact 11581 ggtggccggt gggagatcga tacgcatgga cttttcgtag cgagtggtgg cctttcctgt 11641 aatggctcca accaatacct cctgatacgc cccgccgtcg gtggcgacgt cgaccgcata 11701 ctggatgctg taaccaccaa tattcccttc tgcgtcctgg ctcgccaggc gtggagtggc 11761 caggcgaatg cgagccgccg acagttgcag gttggtcagc gactgaaccc acggcgtgcc 11821 gctgcgtaac tcgatattca gagaggtctc gttgtcgacc gatgggatgc ctggaatgta 11881 ggtctgctcc acagagcccg gccgccagtc ccacttcacg ttggtgaagt tgaaattgcc 11941 gctggcatcc tggatgggag tgttgtcgag gaagatgtcg cgcgcggtgg gcgtgccgtc 12001 aaattcaccc tcccccacgg cgatcaggat cttggccacg ttggtggagc gcaggctgtc 12061 gggtgcctct accggagact tgggcttgct ctcgccacct ttggcaccgt ggatatccag 12121 cttttgtgct gcgcccatgc tttcctccag gcaataaaaa accggctcat ggccggcttg 12181 ctcgatgcgt cgtggttatg tcttgtcttc ggcgtagatc gacgccgaga tgattgcccc 12241 gccccacctg cgatcaccga tgcagatggg gacagggttg ccgctggccg tggtgttctt 12301 tgcgctgccg aatgcatagc tgggcatgtt ttccggtgat gcgctctgct tgaggcccgc 12361 ggcctgaggg ctcagcatct ggatcacgcc gccggccaac agcgcgatac cggcaggcgc 12421 tgtcggcgca ccgaagtagc tggccgcaat gagcacgacg cccagcacaa tctgcaaaat 12481 gccgccgcgc ttgctgccct cgaccactgg gacaatcctg acttccttgg tgccgcccat 12541 gccgaactgg tcaggaccgg tgttcttccg gttgcggaag atggcaaagc gaagtccgag 12601 gcggtcgagt cggcgaatct cagcctcgaa tccgtcaatc gtggccttca gcgcacggaa 12661 tacctcccag gtgtcgcccg atccgacctg gcgccggtgc agcctgccga atttttgagc 12721 cagcgagccg gaaagcttga tggtggtcat cggcgaataa acgatggcgc tcatcgggcc 12781 tccttgtgtc gaagaattaa acgggtgcgc tgcagccatg gcccgccgta gacgatcacc 12841 tctgacgggc ggccatacaa gtgatgcagc acgaaaggac cggggccgaa caccccggaa 12901 tcttcaccgg gcagggctgg gtcagtgccc aagtaaatcc cggcatggtt cgggtgagcc 12961 gttcggccaa cctccatcac gatcatgtcg ccacgcttag ggctgtcgac ctgctcgaac 13021 ccggcggcgg cgtagttcgc ctcgtacaga ctggtgctgt ctgcactctc ccaccagcca 13081 tcggcgcgat ggaaggcttc gaactccaat ccccactccc gctgatacca gtcggcgcaa 13141 acctgccagc agtcccaggc gccgtgcacg aacgggcgct tgagcagcgg cgtgctgccg 13201 gtgggcgtga tggtgcgaag gtcgccttca ggccatgaca ggatgtgcca gggcaaggcc 13261 gtggcctcgc acatggccag gtcgcgcgat gacggccggc tggtggcgtc cgggtgcgag 13321 tggacgatgc caatcacctc accctggtct tccgccgcag cgtagtcctc tggttcgagc 13381 ctgaactctt cgttaggctc ggtggcgatg ttccggcacg ggaagtactt ctgcgctcgg 13441 cccacagcca gcaccaggcc gcagcactct ttcgggtact cggccgccgc atgcgcctgg 13501 atggccgcaa tgatgtgttt gcgcattctt cagctccggg caatgagtga cacggcgggg 13561 aatcccccga acgggtaagg gtttccctcg ccgaagcgag gaatgcagcc cttgccgagg 13621 gtggcgtcgc acacgtccag ttcaggattg tcggtgacga taccgtcgat ggtgacgtat 13681 gggcctgtgt atccacagtt tgggcctctg taaccgcccg tcaggcacca atggcacagc 13741 gtggtcatct gccggccaat ggtttcgccg ccgacatcgc caggactggc caagtcccag 13801 ctgaccgttt cgccgtcttc gttggtcttc tggtcgacat accagacctc gatcgattcc 13861 tgggtgggat cggccgttgg gtttccgcct tcgaagttca ccgcgtccag gtactcggcc 13921 agcgtgctac gaatggtcag cttgaattcg agaagatcct cgaaggccag gcagagcgcg 13981 gtgatacgcc cgttcacgtt gccgaccgac agcttgggcc gcaccgcagt gccgtcgccg 14041 tttgcctcgc tgccctcgta ctgcatgggc caggcgccgt actcctcgcc cttccaccag 14101 atcgacttgg ccggcagctg gtcagcgttg ccgccggcag ccatcagttc agcaggagtg 14161 tgtgggatcg catgaccgtg gaaacgcagc acatcggcgc cgtaatcgct gccgtccagc 14221 tcaaacagca tgacttcgtt accaggctca agagtctgga tagcattgat cagcgacatg 14281 gattgacctt tacgggtgga aagcgcgctc gaacgtcgcc gtcactttaa atacgccccc 14341 gcccatgggc gtaggggttg ggttcttgca ggtgaaaagg ccaagctggc ccagtggcgt 14401 ggtccacaag aaggccttgg cgccctggtg ccggtcgaag aacgccataa tttccagcgc 14461 tctcgcttgg ccgccggtgt gggtaaccgg gtaggaatcg actttgttgt tgatcccgcc 14521 cccgacctct tgcttgtagc cgtccccgaa ctgcgaggtg cgcacccgat aggtaatatc 14581 gggcgcctcc ccgtgctggg tggcccaggt gaacgtttca atcgccatgg ttacctcccg 14641 ttgatggctc gccagatagc cccgccaggc tgaacgccgc gcgcaatggc tgcctcagct 14701 tccgctttgg tggcctgctg gatgttccga ccggcctgcg tggtgtcttc ttgtgtcgcg 14761 gtgccgcctt cgccagccgt ttgtacggag accgagacgg ggaagttgta ggtgtttcca 14821 ccgcctcccc cgccaccacc gccgacgccc agcgcaagca cggcaggcgc agccgaccga 14881 caacaacaaa //