LOCUS MF417966 14492 bp DNA linear ENV 27-FEB-2018 DEFINITION Uncultured Caudovirales phage clone 9AX_1, partial genome. ACCESSION MF417966 VERSION MF417966.1 KEYWORDS ENV. SOURCE uncultured Caudovirales phage ORGANISM uncultured Caudovirales phage Viruses; dsDNA viruses, no RNA stage; Caudovirales; environmental samples. REFERENCE 1 (bases 1 to 14492) AUTHORS van Zyl,L.J., Abrahams,Y., Stander,E.A., Kirby,B.M., Clavaud,C., Farcet,C., Breton,L. and Trindade,M.I. TITLE Novel phages from South African skin metaviromes JOURNAL Unpublished REFERENCE 2 (bases 1 to 14492) AUTHORS van Zyl,L.J., Abrahams,Y., Stander,E.A., Kirby,B.M., Clavaud,C., Farcet,C., Breton,L. and Trindade,M.I. TITLE Direct Submission JOURNAL Submitted (29-JUN-2017) Biotechnology, University of the Western Cape, IMBM, C2, Life Science Building, Robert Sobukwe Rd, Cape Town, Western Cape 7550, South Africa FEATURES Location/Qualifiers source 1..14492 /organism="uncultured Caudovirales phage" /mol_type="genomic DNA" /isolation_source="healthy skin from underarm of human male" /db_xref="taxon:2100421" /clone="9AX_1" /environmental_sample gene 93..875 /locus_tag="9AX1_1" CDS 93..875 /locus_tag="9AX1_1" /note="Similar to YP_164271.1 [Pseudomonas phage F116] (BLASTp score : 140). Similar to PB000740 [Pfam-B_740] (hmmscan score : 109.6)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72645.1" /translation="MVRAILEGRKTVTRRALNAQALKNIGYGVQLGECHELPSEGPLH PNSIGYYIDFCPFGQLGDRLWVRETWQDVHPVQVIDRYSQLGRAGIPGPPGVTYHTIY RADGEYPKVHYTHEHPYRCIEPDPNHGFLGAADSGWTGWTPSIHMPRWASRILLEITD VRVERLQDITEAQAKAEGCFFTDYGQACFHGGRGIRDATECEYPASKHQQRPGWMWDK TNSYEQCLDSARHAFGNLWNSVGGDWEDNPWVWVVEFKRVTP" gene complement(1167..1415) /locus_tag="9AX1_2" CDS complement(1167..1415) /locus_tag="9AX1_2" /note="Predicted by MGA" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72646.1" /translation="MDVSHNFDAPIAHAYRGHVMFLKFDWRRPNDQSPVAAKIIEPAP INGLGEVAAELLGPWPDYPAALDDAMAAAERWIDSQLP" gene 1544..1753 /locus_tag="9AX1_3" CDS 1544..1753 /locus_tag="9AX1_3" /note="Predicted by MGA" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72647.1" /translation="MKLTLILLGALLYLVGTLGWFGWFAIDLLDTGTTEALLYAFAGT CAWLLISFGLAIHIIKTARPTVGGR" gene 1756..1989 /locus_tag="9AX1_4" CDS 1756..1989 /locus_tag="9AX1_4" /note="Predicted by MGA" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72648.1" /translation="MAKVLAQITVKLPRLMEAGEYRKLRYVGGKPSLQQLKKWIEEGE VIGEVKGGMYFVDVQAAVMGSSDPLLAKMLEIG" gene 1989..3125 /locus_tag="9AX1_5" CDS 1989..3125 /locus_tag="9AX1_5" /note="Similar to YP_007111602.1 [Enterobacteria phage mEp235] (BLASTp score : 106). Similar to PF00589.17 [Phage_integrase] (hmmscan score : 45.9)." /codon_start=1 /transl_table=11 /product="putative integrase" /protein_id="ASN72649.1" /translation="MAARPRTLQNRKLPPNLYPNGKYWRYRNPVTGVMTSINRPLEEA IKLARAANLKMAELVVDDGSLLAVLTGDRLPIVSNLLTRFEEDWLPHRSYAVRTLEEI KFKLERYRQDLGDRLIGQLDVLAMAEYLDNFSNNAYTKHRGLWVQIFAFAVAKGLAER NNAELTLVKKEAEKKRQRHTLDGLKVIINAATTPPWLKRAIRLALASLQRRDDIVTWL KSAADMEKNTLTVSPGKTQGYDNPVHLKITMGATLREVVGECLRSPLASPYLIHYKPK ARRREQIDAKDHWTSVTPDYLTKEFSKARDAAHAYDHVPAGERPTFHEIRALGAWLYE QQNFPQEYIQALMGHADEKMTKHYQEGHDEKKIEYVEVGAELAF" gene 3496..3855 /locus_tag="9AX1_6" CDS 3496..3855 /locus_tag="9AX1_6" /note="Predicted by MGA" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72650.1" /translation="MNVDMDTDDWLGCPTPLEMYQHQCSILVDELVETERMLRRARAN IAGLVQMNDLLMTGKDQAETSLKDALSQVAALNLETSRQGRKLNGLAIITEQRDHLLR ENQRLLLELSVYKQPLA" gene complement(3847..4056) /locus_tag="9AX1_7" CDS complement(3847..4056) /locus_tag="9AX1_7" /note="Predicted by MGA" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72651.1" /translation="MAINVDQVNAMEAWFALRNDPTFISATPEERYETRLALADDLKQ QGLINDGEWRELTEEAVAAYADELG" gene 4140..4820 /locus_tag="9AX1_8" CDS 4140..4820 /locus_tag="9AX1_8" /note="Similar to YP_164331.1 [Pseudomonas phage F116] (BLASTp score : 338). Similar to PF02586.9 [DUF159] (hmmscan score : 140.7)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72652.1" /translation="MCGRLSQYRGIHDFVAALSMPNALANSVGDQPIERYNVAPTTAV ALLHLKGDLLHADPVRWGWRPHWAKDRAAPINARVEKVAHGPFFRAIWPHRAITPIDN WFEWVDEGGPKKQPYLIRRRDGAPIYCAAIGQLPDADEGPGEHDGFVIITADSAGGMV DIHDRRPVVLTPDLAREWLDPATLKERAEQMVLHQGEPSDVFEWFKVSMAVGNVRNKG PELIRPLG" gene complement(4974..5489) /locus_tag="9AX1_9" CDS complement(4974..5489) /locus_tag="9AX1_9" /note="Similar to YP_006590002.1 [Burkholderia phage DC1] (BLASTp score : 61.2). Similar to PF03245.8 [Phage_lysis] (hmmscan score : 84.6)." /codon_start=1 /transl_table=11 /product="putative Rz" /protein_id="ASN72653.1" /translation="MTSVQKLAGLVVLILVLMASAAGVTWQVQDWRMGNKLAQQAVLH GDDLAAISNAATAQARAEQGKRLGLEQQLAGQDQQHTKELSDAQRNQARLRDQLATAD VRLSVLLAEDSASGCNVPTTPGTAGVVHAARRAQLDPAHSQRIIRITDDGDNAIIALR ACQAYVRAIAR" gene complement(5486..5911) /locus_tag="9AX1_10" CDS complement(5486..5911) /locus_tag="9AX1_10" /note="Similar to YP_007392728.1 [Pseudomonas phage JBD5] (BLASTp score : 140). Similar to PB000783 [Pfam-B_783] (hmmscan score : 177.4)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72654.1" /translation="MSITETRGVRNNNPGNIDYNPANQWQGQLKADPALEKRFARFDT PENGIRALGKLLLTYQRKHGLKTVKAIISRWAPSVENDTAAYVRAVEANTGTRPGAEI DLVNPVVMAGFVKAIIHHENAGYAYPDAVLAEGVRRALA" gene complement(5955..6956) /locus_tag="9AX1_11" CDS complement(5955..6956) /locus_tag="9AX1_11" /note="Similar to YP_004934057.1 [Escherichia phage HK639] (BLASTp score : 156)." /codon_start=1 /transl_table=11 /product="putative tail protein" /protein_id="ASN72655.1" /translation="MARQGINLGTAPTGQGGDTFRTASQKNNDNSSELYTALGAPANG QLPAALPVAKGGTGGTTQLTARQGLGLGAASTKSFGLQDGELIPAGVLSGMFSNVAPD AYQMDRPGEPGQQGAFYKFLNNGSSSGLSYSTLLRLPYNTGYEAQIFIPMGTSQLAFR TVTGAAGTFGPTCSVYHTGNTTRGSGGALSAASPILRIANVSASERRDLQEESFLPAG EWGVANDEARGVIVERLGVGEYRVTGSLGLALEGWRTHDPSSPDGGRMLGITDSHQEE DGTVIIRLFKQRWTLTDDGEMVPGRGAPMDVPLNSWIDVRLEMPKVDTPPPLRSTEE" gene complement(6985..7314) /locus_tag="9AX1_12" CDS complement(6985..7314) /locus_tag="9AX1_12" /note="Predicted by MGA" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72656.1" /translation="MWGADGKQIFDSAVQAAVFTRVAQNWTFTHAEQSGQGLITNWYS VPLNYALGDYLMINNARMPMMAGNNESRGTGLRYDFPAGLIRFSVTTVTNPTYFSLSA VFGKLAV" gene complement(7958..11791) /locus_tag="9AX1_13" CDS complement(7958..11791) /locus_tag="9AX1_13" /note="Similar to YP_007112295.1 [Enterobacteria phage HK633] (BLASTp score : 886). Similar to PF13550.1 [Phage-tail_3] (hmmscan score : 70.4)." /codon_start=1 /transl_table=11 /product="putative central tail fiber" /protein_id="ASN72657.1" /translation="MGAAQKLDIHGAKGGESKPKSPVEAPDSLRSTNVAKILIAVGEG EFDGTPTARDIFLDNTPIQDASGNFNFTNVKWDWRPGSVEQTYIPGIPSVDNETSLNI ELRSGTPWVQSLTNLQLSAARIRLATPRLASQDAEGNIGGYSIQYAVDVATDGGAYQE VLVGAITGKATTRYEKSMRIDLPPATSGWLIRVRRITPNQNTDKIADSLFIAGYTQVI DAKLRYPNTALLFIEFDAEQFTNIPAVTVKCKARRWQVPSNYDPVARTYSGAWDGTMK EAWTNNPAWITYGICTQDRFGLGRRIKPWMVDKWELYRIAQYCDQLVSNGADGVEPRF LCDMNLQGKADAWSLLRDIAGIYRGMTYWAQGQLVMQADMPRAQDIDYVFTRSNVIDG KISYGSASAKTRFTRCLVSYDNPLNNYDTDVTVYSDLPLQRRLGDKPTEISAIGCTRA SEAQRRAKWLVLSNNQDRTISFRTGMEGRIPLPGFIIPVADSLLAGREIGGRIAAAVG KVITLDRDTLAKAGDRLVINLPGGRAEGRTVESVSGRNVTVTVAYSEAPAAQLQWAID ADDLAIPLYRVMRTARTPEGDYDISALQYEPSKFPSIDTGARLEERPISVIPITVVPP PASVTVTSNVSIDQGLAISTMNISWPAVAGAVAYDVEWRKDSGNWIKVQRTGSTSVDV TGIYSGAYLARVRSVSAFEISSIWKSSTLTSLEGKTGLPPAVSSLTTKGELFGISIKW GFPAGAEDTQRTELWYGPANNLEAATKLADLAYPQADYRMQSLLSGATLFFWARLVDR TGNIGPFYPTVNGVMGQSVSDAWPLLEQVKGQIDESSLAPALNGRIDLIDGDGPGSVN ARNEQLRKDLEEQIKPYVDALLWDAAKAYVKGDIVRQGNKLYQALEANSGSQPPSENW KDVGDILTETNALAIRVDNLDQEITAVDGRVVATQEQLTQLQTKVNDPVTGLAATVNN VTQVNQRVTNVDNKLTAQTERIDGVYAEINAPMAGSENDLAGGTQGYAGVWSIQSAIT DGDYAQGKLTQTVEAQVIKNYATYEETTTALVNQQTATTSQVTALRSDFDGNKSTVQT QIKTVSDQASATASQTTQLQTTVNGHTQQFQQQASINQDVNGKITGTYSFRFKFDVNG APYSTGVGLGIETGANGQVTSRFVIQADQFAIYNGEVGAAGSSVPFAVNGTETYIKSA FIQDGTITNAKIGSYIQSNNYVAGQTGWKLFFDGTFEINGVAPGQGRSMMTNRSLRFW DANNVKRLQLGDQTE" gene complement(11849..12427) /locus_tag="9AX1_14" CDS complement(11849..12427) /locus_tag="9AX1_14" /note="Similar to YP_009004652.1 [Edwardsiella phage eiAU-183] (BLASTp score : 172). Similar to PF06805.7 [Lambda_tail_I] (hmmscan score : 42.2)." /codon_start=1 /transl_table=11 /product="putative tail assembly protein" /protein_id="ASN72658.1" /translation="MSAIVYSPMTTIKLSGSLAQKFGRLHRRQVGSGDTWEVFRALKA TIDGFEAEIRRLDRLGLRFAIFRNRKNTGPDQFGMGGTKEVRIVPVVEGSKRGGILQI VLGVVLIAASYFGAPTAPAGIALLAGGVIQMLSPQAAGLKQSASPENMPSYAFGSAKN TTASGNPVPICIGDRRWGGAIISASIYAEDKT" gene complement(12424..13179) /locus_tag="9AX1_15" CDS complement(12424..13179) /locus_tag="9AX1_15" /note="putative gp19; Similar to NP_037679.1 [Enterobacteria phage HK022] (BLASTp score : 234). Similar to PF14464.1 [Prok-JAB] (hmmscan score : 82.6)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72659.1" /translation="MRKHIIAAIQAHAAAEYPKECCGLVLAVGRAQKYFPCRNIATEP NEEFRLEPEDYAAAEDQGEVIGIVHSHPDATSRPSSRDLAMCEATALPWHILSWPEGD LRTITPTGSTPLLKRPFVHGAWDCWQVCADWYQREWGLEFEAFHRADGWWESADSTSL YEANYAAAGFEQVDSPKRGDMIVMEVGRTAHPNHAGIYLGTDPALPGEDSGVFGPGPF VLHHLYGRPSEVIVYGGPWLQRTRLILRHKEAR" gene complement(13183..13932) /locus_tag="9AX1_16" CDS complement(13183..13932) /locus_tag="9AX1_16" /note="Similar to YP_007111390.1 [Enterobacteria phage mEp237] (BLASTp score : 270). Similar to PF05100.7 [Phage_tail_L] (hmmscan score : 221.0)." /codon_start=1 /transl_table=11 /product="putative minor tail protein L" /protein_id="ASN72660.1" /translation="MSLINAIQTLEPGNEVMLFELDGSDYGADVLRFHGHAIPHTPAE LMAAGGNADQLPAKSIWWKGEEYGAWPMQYEGSEANGDGTAVRPKLSVGNVNGRITAL CLAFEDLLEFKLTIRSTLAEYLDAVNFEGGNPTADPTQESIEVWYVDQKTNEDGETVS WDLASPGDVGGETIGRQMTTLCHWCLTGGYRGPNCGYTGPYVTIDGIVTDNPELDVCD ATLGKGCIPRFGEGNPYPFGGFPAVSLIARS" gene complement(13943..14281) /locus_tag="9AX1_17" CDS complement(13943..14281) /locus_tag="9AX1_17" /note="Similar to YP_007151622.1 [Enterobacteria phage HK544] (BLASTp score : 112). Similar to PF05939.8 [Phage_min_tail] (hmmscan score : 131.0)." /codon_start=1 /transl_table=11 /product="putative minor tail protein" /protein_id="ASN72661.1" /translation="MAIETFTWATQHGEAPDITYRVRTSQFGDGYKQEVGGGINNKVD SYPVTHTGGQARALEIMAFFDRHQGAKAFLWTTPLGQLGLFTCKNPTPTPMGGGVFKV TATFERAFHP" ORIGIN 1 gtaacccctc ccccttcaaa gtcagccgct atagcggcaa ggaacccgcc atgcctgaaa 61 taaaggaacg gccaatactc ttttcggcac cgatggtgcg cgccatcctg gaaggccgga 121 agacggtcac gcgccgggcg ctgaatgcgc aagcgttgaa gaacatcggc tacggagttc 181 agcttggcga gtgtcacgaa ctcccgagcg aagggccgct acacccgaac agcatcggct 241 actacataga cttctgcccc ttcggacaac tcggggaccg tctgtgggtg cgcgagactt 301 ggcaagacgt tcatccggtt caagttattg accgctactc acagcttggc cgcgccggga 361 tacctggccc tccaggcgtg acctaccaca caatctatcg agcagatggg gaatatccga 421 aagtccacta cacacatgaa cacccctacc gctgcatcga acctgaccca aatcacggtt 481 ttctcggcgc agcagattca gggtggacag gctggacccc cagtatccat atgccacgct 541 gggccagccg catcctgctg gagatcaccg acgtgcgggt cgagcggttg caagacatca 601 ccgaggcaca ggccaaggcc gaaggatgct tcttcaccga ctacggccag gcctgcttcc 661 atggtggacg ggggatacgc gatgcaaccg agtgcgaata ccccgccagc aaacaccaac 721 agcgcccagg ctggatgtgg gacaagacga acagctacga gcagtgcctt gactcagccc 781 gccacgcttt cggcaaccta tggaactcgg tcggcggcga ctgggaggac aacccgtggg 841 tctgggtggt cgagttcaag cgggtgacgc catgatcttg ctcgcagcac ctgcagccct 901 ctggttcgcc tacgtcttca tctacaaggg gccgaggcca tgatcacctt ggagcaaatt 961 cgggctgagg tggctgaccg aaaaaccgta gatcaccaca cccgctgccg tgattgcggc 1021 cacttcgtac cgaaagagcg atgggtgccg agggaatccc cacatgccgc acgcgggcag 1081 cgtccactct gtgcaccctg cttcgacgaa tacgaaggcc cactctacta accccaatcc 1141 ccctacatgc ctgccggtga gcggagtcag ggcaactggc tgtcgatcca tcgttcagct 1201 gccgccatcg catcatcaag tgctgccgga tagtcaggcc aagggcccaa caactctgca 1261 gcaacctcac ccaagccatt gataggtgct ggctcaatga tctttgcggc tacagggctc 1321 tgatcgttcg ggcgacgcca gtcgaacttg agaaacatca cgtggccccg gtaagcgtgc 1381 gctatcggag catcgaagtt gtgtgacacg tccatgcctc atcacgaact aagttgaacc 1441 cttttgtaca ccgcttcggt cctgtttgaa agataggcag aaagctatca ctccaatccc 1501 ctatatgccg cctcgcgtgg ctaggacaca ccccatgttc gctatgaaac tcaccctgat 1561 actgctgggc gctttgctgt acctggtagg aacactcggc tggttcggct ggttcgctat 1621 agacctgctg gacaccggca ccaccgaggc actgctctac gccttcgccg gcacctgcgc 1681 ctggctgttg atcagcttcg gcctggcaat ccacatcatc aagacagcgc ggcccacggt 1741 gggcgggagg tagctatggc aaaagtcctg gcgcaaatta cggtcaagct gccgcgcctc 1801 atggaggccg gcgaatacag gaagttgcgg tacgtcggtg gaaagccgag cctgcagcag 1861 ttgaaaaaat ggattgagga aggcgaagtg attggagagg taaaaggcgg gatgtatttc 1921 gtcgacgtgc aggcggcggt tatggggtct agtgacccgc tgctggccaa gatgctggag 1981 attgggtgat ggctgcccgg ccccgtacgc tgcaaaacag aaagctgccg ccaaacctct 2041 acccgaacgg gaagtactgg cggtaccgca acccggtcac cggcgtgatg accagcatca 2101 accgcccact ggaggaggca atcaagctgg cccgggcggc caacctcaaa atggccgagt 2161 tggtcgtaga tgacggctcg ctgctggcgg tgttgaccgg cgaccgccta ccgatagtga 2221 gcaacctgct gacgcgtttc gaagaagatt ggttgccgca ccgctcatac gccgtacgca 2281 ccctggagga gatcaagttc aagctcgagc ggtaccggca ggatctgggt gaccgcttga 2341 taggccagtt ggatgtgctg gccatggccg agtacctgga taacttcagc aacaacgcct 2401 acacgaagca ccgcgggctg tgggtgcaga tattcgcgtt cgcagtggcc aagggcctgg 2461 ccgaacgcaa caacgccgaa ctcaccctgg tgaagaagga ggcagagaaa aagcgccagc 2521 gccatacgct cgacgggttg aaggtgatca tcaatgcagc aacaacgccg ccatggctaa 2581 agcgggcaat ccgcctggcc ctggccagcc ttcagcgccg ggacgacatc gttacctggc 2641 tgaagtctgc tgcggatatg gaaaagaaca cgctcacagt gtcgccaggg aaaacccagg 2701 gttacgacaa cccggtacac ctgaagatca ccatgggcgc aacgctgcgg gaggtggtcg 2761 gagagtgcct gcgctcgccg ctcgcctcgc cctacctgat ccactacaag cccaaggccc 2821 gccggcgaga gcagatagac gccaaggatc actggacatc ggtaacgccg gactacctga 2881 ccaaggagtt cagcaaggcc agggacgcag cgcacgccta tgaccacgtg ccggccggag 2941 agcgccccac ttttcacgag atccgcgcat tgggtgcctg gctgtacgag cagcagaact 3001 tcccacagga gtacatccag gcgctcatgg gccatgcgga cgagaagatg acgaagcatt 3061 atcaggaggg gcacgacgaa aagaagatcg agtatgtcga ggtgggcgcc gagttggcgt 3121 tttgaggtgg gggttttgca aaagttttgc aaaagttttg caaatcgcag aaagcaaaaa 3181 ggggtcaccg tttccgatga cccctctaga ccgcccagca gagcggattt tgtttggtag 3241 gcgcgattgg actcgaacca acgaccccca ccatgtcaag gtgaccccgg aacgctcgta 3301 agtatttgat atatatagaa atcacgccta ttcaggctag gccaaaaaca agcttatagc 3361 cctataagaa tcaataactt agcgttggat attgctacag tgattccctt cccggcgtcc 3421 tgccgacgaa caccactccc caaacccgca acgcctcgat tactgtacac acatacagta 3481 catgagattc acgctatgaa cgtagacatg gacaccgatg attggctcgg ctgccccact 3541 ccgctggaga tgtaccagca ccaatgctca atcctcgtag acgagctggt ggagaccgag 3601 cgcatgctgc gtcgagcgcg ggcgaatatc gccggcctgg tgcagatgaa tgatctgctg 3661 atgacaggaa aggatcaggc ggagacctcc ttgaaagacg ccctatccca agtagctgcc 3721 ctgaacctgg aaacctcacg ccagggccgc aagttaaacg ggcttgcgat aatcacggaa 3781 cagagggacc accttctcag ggaaaaccag cgactactgc ttgagcttag cgtttacaag 3841 cagccattag cctagctcgt cggcataggc cgcaactgct tcctcagtca gctctcgcca 3901 ctcaccatcg ttgatcagcc cctgttgctt gaggtcgtca gccagggcca gccgcgtttc 3961 gtagcgctcc tcaggcgtgg ccgatatgaa ggttggatcg ttacgcagcg caaaccacgc 4021 ctccattgcg ttcacctgat caacgttgat cgccatgacg aatacctcgg tccagtgtct 4081 acagtgtaga gattggccgg ggaccggctg ttcatcggcg ccgacgagcg gagatgctta 4141 tgtgcgggag actttcgcag taccggggaa tccacgattt cgttgctgct ctgagcatgc 4201 ccaatgctct ggcgaactcc gtaggcgatc agccgattga gcggtacaac gtggcaccga 4261 caaccgcggt tgcactgctt cacctgaagg gcgacctact ccacgccgat ccggttcgct 4321 ggggctggcg gccgcattgg gcaaaagacc gggcagcgcc gatcaatgcc cgcgtggaga 4381 aggtagccca cggcccgttc ttccgggcga tctggccgca ccgggcgatc acgcctatag 4441 acaactggtt tgagtgggtg gatgagggcg ggcccaagaa gcagccctac ctcatccgcc 4501 ggcgggatgg tgcaccaata tactgcgctg ccattggcca actgccggac gctgatgaag 4561 gaccgggcga gcatgacggc ttcgtcatca tcaccgcgga cagcgccggg ggaatggtgg 4621 acatccacga ccggaggccc gtggtgctga cgcccgacct ggcccgggaa tggttggacc 4681 cggccacgct caaggagcgc gccgagcaaa tggttctgca ccagggcgag ccgtccgacg 4741 tgttcgaatg gttcaaggtc agcatggccg tgggtaacgt gcggaataag ggaccagaat 4801 tgatacgacc tttaggttaa ttgtcgtaga aataccacat gacagacgcc actgtgacga 4861 cccatccaag ggtgagcagt attgacagcc ccgcaagccg cttatccatg gtgcccttca 4921 gttaccaaaa tgatcgaagt tagagcgcac ccggcaatat tagctcaacg cactcatcgt 4981 gcaattgcgc ggacataggc ctggcaagca cgcaaagcga ttatggcgtt atccccgtca 5041 tcggtgatgc ggataattct ttgagaatgc gctgggtcaa gttgggctcg acgggctgca 5101 tgaaccacgc cggcggtgcc gggggtggta ggcacgttgc agccactggc tgaatcctcg 5161 gcaaggagga ctgacagccg gacatcagca gtagcaagct ggtcacgcag gcgagcctgg 5221 ttgcgctggg catcggataa ttcctttgta tgttgttggt cctggccagc gagctgctgc 5281 tccagaccca ggcgcttgcc ctgctcggcg cgggcttggg cggtggcggc attactgatc 5341 gcggcaaggt catccccgtg caggacggcc tgctgggcga gcttattgcc catccgccag 5401 tcttgaacct gccaggtaac gcccgcggca ctggccatca gcaccaggat cagcaccacc 5461 aggccggcca gcttctgtac cgacgtcatg ccagcgcccg ccgcacgcct tctgccaaca 5521 ccgcatcagg gtaggcatat ccggcgttct cgtgatggat gatggctttg acgaagccag 5581 ccattaccac cgggttgacc aggtcaatct cggcgccagg ccgggtgccg gtgttcgctt 5641 cgaccgcgcg cacgtacgcc gcagtgtcgt tctctaccga aggcgcccac cggctgatga 5701 tcgccttcac agttttcagg ccatgtttgc gctgataagt cagcaggagc ttgcccaggg 5761 cgcggatacc attctccggc gtgtcgaacc ttgcgaagcg cttctctaaa gcagggtctg 5821 ccttcagctg gccctgccac tggttggccg ggttgtaatc gatgttgccg gggttgttgt 5881 tgcgtacccc gcgagtttcg gtgatcgaca tgcttttctc caggcgtaaa aaagcccgct 5941 caattggcgg gctgctattc ttcggtcgat ctgagtggcg gcggcgtatc gaccttgggc 6001 atttcaagcc gcacatcgat ccagctattc agcgggacat ccattggcgc gccacggccc 6061 ggcaccatct ctccgtcatc ggtgagcgtc cagcgctgtt tgaataggcg aatgatgaca 6121 gtgccatcct cttcctgatg actgtcggtg ataccaagca tccggccgcc gtctggtgag 6181 cttgggtcgt gggtccgcca gccctccagc gccaagccaa gacttcccgt cacccgatac 6241 tcgccaacgc cgaggcgctc aacaataacg ccgcgtgctt catcgttggc cacaccccat 6301 tcgccggcgg ggagaaacga ttcctcttgg aggtcgcggc gctcgctggc ggacacgtta 6361 gcgattctca ggatcggcga tgccgccgaa agcgcgccgc ctgagccgcg tgtggtgttg 6421 ccagtgtgat acacactgca tgtcgggcca aatgttccgg ctgctcccgt gacagttctg 6481 aatgcgagct gactggtgcc catagggata aaaatttgcg cctcgtagcc ggtgttgtat 6541 ggaaggcgta acagtgttga gtagctcagc ccagacgaag atccgttatt caaaaacttg 6601 tagaaagcgc cttgttgccc gggctcgcca ggtctgtcca tttgataggc gtccggtgcg 6661 acattggaga acatcccact gagaacgccc gccgggataa gctctccatc ttgcagccca 6721 aagcttttag ttgaggcagc gcctagccct aagccttgcc gggcagtcag ctgcgtagtc 6781 ccacccgtgc cgccttttgc aacgggcagc gccgcaggca gctgtccgtt agccggagct 6841 ccaagggcag tataaagctc gctcgagttg tcgttatttt tctggctggc agtacggaac 6901 gtatcgccgc cctggcctgt aggcgccgta ccaagattaa tgccttgtct tgccattgaa 6961 ttaaacccct atacgaaatg aaaattaaac agccagcttc ccaaataccg cagagagcga 7021 aaagtatgtt ggattcgtca cggtagtaac gctaaatctg ataagcccgg ccgggaagtc 7081 atatcgcaga cccgttcctc tagattcatt gtttcccgcc atcatgggca tccttgcatt 7141 attgatcatc aagtaatcac ctagggcgta gttaagagga accgaatacc aatttgttat 7201 tagcccttgt ccgctctgct ccgcgtgcgt aaatgtccag ttttgcgcga cccttgtgaa 7261 tactgccgcc tgaacagcag agtcaaatat ctgttttcca tccgcacccc acattcttag 7321 gccatatgtg gacaaaggct tggatgaaaa cgcagctacg aatatgtttc cagagtgaac 7381 ctgcccagag gtaactgtgg cgccggtcca cgcccccgcc gatccttcaa gaagaacgcc 7441 aagctgaatc agcgagccat tatttggcgg cctgataaaa acaaggggcg gctcctgcgt 7501 gtcaaccaca ttctgaaaga cgacggacgc gccagagtta taggtgccct tatgaaagac 7561 acacaacctc gcatattcag agtctaggga tatagcgcca gaatcattca ccacttgcag 7621 tccgtaactc atcacgccca ccttgcaaca atgagacgca tggttgccga agagaatgaa 7681 tcggaaggac gagctttgag aaaattccta acataaactt cgttcgtgcc catttcgcac 7741 tccaactgcc tgttagatac gacctgcgag ttatcgttat tgatcggtaa caaaaagcat 7801 atcgcgttgt ttgtggtgca ccctggcgct gcaaaggtcc tagtgcccag cggtcctgag 7861 gaataggaca caagtaccga cagaacaatc ctataagtgg atatgcttgt atcgaactca 7921 agggtgccgt taggagacca aattcttaag ccggaactca ttctgtttgg tctcctaatt 7981 gcagtctttt gacgttgttt gcgtcccaaa agcgcagaga tctgttcgtc atcattgatc 8041 gcccttgccc aggagctaca ccgttgattt cgaaggttcc gtcgaaaaac aatttccagc 8101 ctgtctgccc tgcgacgtag ttgttcgact ggatgtagct acctatcttc gcgttggtta 8161 tcgtcccgtc ctggatgaat gccgacttga tataggtttc tgtgccgttc accgcgaatg 8221 gcacgcttga ccctgccgcc cctacctcgc cgttgtagat tgcaaactga tccgcctgga 8281 tgacgaagcg cgaagtaacc tgaccgttcg ccccagtctc aatgccaagt cctacgccgg 8341 tagagtaagg cgccccattc acatcgaact tgaatcggaa cgagtaggtg ccggtgatct 8401 ttccgtttac gtcttggttg atgcttgctt gttgctggaa ctgctgcgtg tgtccgttga 8461 cggttgtttg cagctgagta gtctggcttg cggtggctga cgcctgatcg gataccgtct 8521 taatctgcgt ctgtacggtg gacttgttcc cgtcaaaatc tgagcgaagc gccgttactt 8581 ggctggttgt agcggtctgc tggttgacga gcgcggtcgt tgtctcctca taggtggcgt 8641 agtttttgat aacttgagct tcgacagttt gagtcagctt gccttgggcg tagtcaccat 8701 cggtaatagc agactggatc gaccaaacgc ccgcatagcc ctgggttccg ccagccaggt 8761 cattctcaga accagccata ggcgcgttga tttcagcgta aacgccatca attcgctccg 8821 tctgcgcggt cagcttgtta tcaacgttgg ttactcgctg attcacttgg gtaacgttat 8881 ttaccgtagc cgccaggccg gttaccgggt catttacctt tgtctggagc tgggtaagct 8941 gttcctgagt ggcaaccacc ctcccatcga ctgcggtgat ttcctggtca aggttatcaa 9001 cccgtattgc caaagcgttc gtttcagtga ggatgtcgcc gacgtctttc cagttttcgc 9061 tgggaggctg cgagccactg ttagcctcca gggcttggta cagcttgttt ccctggcgaa 9121 cgatgtcacc cttgacgtaa gcctttgctg catcccacaa cagcgcatca acgtatggct 9181 tgatttgctc ctctagatcc tttctaagct gctcatttcg agcgtttacg gagccaggcc 9241 catctccgtc aatcagatcg atgcgaccgt taagggctgg agccaacgat gattcatcaa 9301 tctgcccctt gacctgctca aggagcggcc aagcatccga aacagactgt cccatcactc 9361 cgtttacggt cggatagaaa gggccaatgt tcccggtacg atcaaccagg cgcgcccaga 9421 agaacagcgt agccccggat aaaagcgact gcatacggta gtcggcctgc ggatatgcca 9481 ggtcggccag cttcgtcgcc gcctccaggt tgttcgcagg cccataccac agctcggtcc 9541 gctgggtgtc ctcggcgcca gcagggaagc cccacttgat gctgatcccg aacagctctc 9601 ctttagtggt aagggacgaa actgccggcg gcaggccggt cttgccttca aggctggtca 9661 gggtagagct tttccagatc gacgaaattt cgaaggcgct caccgaacgc acacgtgcga 9721 gataggcgcc cgagtagatg ccggtgacgt caacgctcgt cgagccggta cgctgcacct 9781 tgatccagtt gccgctgtcc ttacgccact ccacgtcata ggcgacggcg ccagcgacgg 9841 ctggccacga gatgttcata gtactgatgg ccaggccctg gtcgatcgat acgttcgacg 9901 taacggtgac gctcgccggc ggcggtacca cagtgatcgg tatcacgctg attgggcgtt 9961 cttccagccg tgctccggtg tcgatgctgg ggaacttgct tggctcgtac tgcaaggcgc 10021 tgatgtcgta atcaccctct ggcgtccggg cagtcctcat aaccctatat agagggattg 10081 ccaagtcgtc agcgtcgatt gcccactgaa gctgtgcagc cggagcctcg ctgtaagcaa 10141 ccgtcacggt tacattgcga ccgctcacgc tttccacggt gcgcccttct gctcgcccgc 10201 cgggaaggtt gatcaccagc cggtcgccgg ccttggccaa ggtgtcgcgg tccaaggtga 10261 tgaccttccc caccgccgct gcgatgcgcc cgccgatctc ccggcccgcc agcagcgagt 10321 cggcgacggg gatgatgaaa ccaggcaacg ggatacgacc ttccatgccg gtcctgaagc 10381 tgatcgtgcg gtcctggttg ttgctcagga ctagccattt tgcccggcgc tgtgcctcag 10441 atgcgcgagt acagccgatg gcactgatct ccgtaggctt gtcacccagg cggcgctgga 10501 gtggaaggtc ggaatagacc gtgacgtcgg tgtcgtagtt gttcagcggg ttgtcatagc 10561 tgaccagaca gcgagtaaag cgggtcttcg ccgatgcgct gccgtaggag attttcccgt 10621 cgatgacgtt ggatcgggtg aagacgtagt cgatgtcttg cgcccgaggc atatcagcct 10681 gcattaccaa ctggccctgc gcccagtacg tcatgccgcg gtaaatacca gcgatgtcgc 10741 gaagcagaga ccaggcgtcg gccttgccct gcagattcat gtcgcacagg aagcgtggct 10801 ccacgccgtc ggcgccgttg gataccaatt ggtcgcagta ttgcgcgatg cggtacaact 10861 cccacttatc aaccatccac ggcttgatac ggcggcccag gccgaaacgg tcctgggtgc 10921 agatcccgta ggtgatccag gccgggttgt tggtccaggc ctctttcatg gtgccgtccc 10981 aggcgccgga ataggtgcgg gccactgggt cgtagttgct cggcacctgc cagcggcggg 11041 ctttgcactt cacggtaacg gccgggatat tggtgaattg ttcggcgtcg aactcgataa 11101 agagcagtgc ggtgttcggg tagcgcaact ttgcgtcgat aacctgggtg tagccagcga 11161 tgaagaggct gtccgcgatt ttgtcggtgt tctggtttgg ggtgatgcgg cgaacccgaa 11221 tcagccagcc actggtggcc ggtgggagat cgatacgcat ggacttttcg tagcgagtgg 11281 tggcctttcc tgtaatggct ccaaccaata cctcctgata cgccccgccg tcggtggcga 11341 cgtcgaccgc atactggatg ctgtaaccac caatattccc ttctgcgtcc tggctcgcca 11401 ggcgtggagt ggccaggcga atgcgagccg ccgacagttg caggttggtc agcgactgaa 11461 cccacggcgt gccgctgcgt aactcgatat tcagagaggt ctcgttgtcg accgatggga 11521 tgcctggaat gtaggtctgc tccacagagc ccggccgcca gtcccacttc acgttggtga 11581 agttgaaatt gccgctggca tcctggatgg gagtgttgtc gaggaagatg tcgcgcgcgg 11641 tgggcgtgcc gtcaaattca ccctccccca cggcgatcag gatcttggcc acgttggtgg 11701 agcgcaggct gtcgggtgcc tctaccggag acttgggctt gctctcgcca cctttggcac 11761 cgtggatatc cagcttttgt gctgcgccca tgctttcctc caggcaataa aaaaccggct 11821 catggccggc ttgctcgatg cgtcgtggtt atgtcttgtc ttcggcgtag atcgacgccg 11881 agatgattgc cccgccccac ctgcgatcac cgatgcagat ggggacaggg ttgccgctgg 11941 ccgtggtgtt ctttgcgctg ccgaatgcat agctgggcat gttttccggt gatgcgctct 12001 gcttgaggcc cgcggcctga gggctcagca tctggatcac gccgccggcc aacagcgcga 12061 taccggcagg cgctgtcggc gcaccgaagt agctggccgc aatgagcacg acgcccagca 12121 caatctgcaa aatgccgccg cgcttgctgc cctcgaccac tgggacaatc ctgacttcct 12181 tggtgccgcc catgccgaac tggtcaggac cggtgttctt ccggttgcgg aagatggcaa 12241 agcgaagtcc gaggcggtcg agtcggcgaa tctcagcctc gaatccgtca atcgtggcct 12301 tcagcgcacg gaatacctcc caggtgtcgc ccgatccgac ctggcgccgg tgcagcctgc 12361 cgaatttttg agccagcgag ccggaaagct tgatggtggt catcggcgaa taaacgatgg 12421 cgctcatcgg gcctccttgt gtcgaagaat taaacgggtg cgctgcagcc atggcccgcc 12481 gtagacgatc acctctgacg ggcggccata caagtgatgc agcacgaaag gaccggggcc 12541 gaacaccccg gaatcttcac cgggcagggc tgggtcagtg cccaagtaaa tcccggcatg 12601 gttcgggtga gccgttcggc caacctccat cacgatcatg tcgccacgct tagggctgtc 12661 gacctgctcg aacccggcgg cggcgtagtt cgcctcgtac agactggtgc tgtctgcact 12721 ctcccaccag ccatcggcgc gatggaaggc ttcgaactcc aatccccact cccgctgata 12781 ccagtcggcg caaacctgcc agcagtccca ggcgccgtgc acgaacgggc gcttgagcag 12841 cggcgtgctg ccggtgggcg tgatggtgcg aaggtcgcct tcaggccatg acaggatgtg 12901 ccagggcaag gccgtggcct cgcacatggc caggtcgcgc gatgacggcc ggctggtggc 12961 gtccgggtgc gagtggacga tgccaatcac ctcaccctgg tcttccgccg cagcgtagtc 13021 ctctggttcg agcctgaact cttcgttagg ctcggtggcg atgttccggc acgggaagta 13081 cttctgcgct cggcccacag ccagcaccag gccgcagcac tctttcgggt actcggccgc 13141 cgcatgcgcc tggatggccg caatgatgtg tttgcgcatt cttcagctcc gggcaatgag 13201 tgacacggcg gggaatcccc cgaacgggta agggtttccc tcgccgaagc gaggaatgca 13261 gcccttgccg agggtggcgt cgcacacgtc cagttcagga ttgtcggtga cgataccgtc 13321 gatggtgacg tatgggcctg tgtatccaca gtttgggcct ctgtaaccgc ccgtcaggca 13381 ccaatggcac agcgtggtca tctgccggcc aatggtttcg ccgccgacat cgccaggact 13441 ggccaagtcc cagctgaccg tttcgccgtc ttcgttggtc ttctggtcga cataccagac 13501 ctcgatcgat tcctgggtgg gatcggccgt tgggtttccg ccttcgaagt tcaccgcgtc 13561 caggtactcg gccagcgtgc tacgaatggt cagcttgaat tcgagaagat cctcgaaggc 13621 caggcagagc gcggtgatac gcccgttcac gttgccgacc gacagcttgg gccgcaccgc 13681 agtgccgtcg ccgtttgcct cgctgccctc gtactgcatg ggccaggcgc cgtactcctc 13741 gcccttccac cagatcgact tggccggcag ctggtcagcg ttgccgccgg cagccatcag 13801 ttcagcagga gtgtgtggga tcgcatgacc gtggaaacgc agcacatcgg cgccgtaatc 13861 gctgccgtcc agctcaaaca gcatgacttc gttaccaggc tcaagagtct ggatagcatt 13921 gatcagcgac atggattgac ctttacgggt ggaaagcgcg ctcgaacgtc gccgtcactt 13981 taaatacgcc cccgcccatg ggcgtagggg ttgggttctt gcaggtgaaa aggccaagct 14041 ggcccagtgg cgtggtccac aagaaggcct tggcgccctg gtgccggtcg aagaacgcca 14101 taatttccag cgctctcgct tggccgccgg tgtgggtaac cgggtaggaa tcgactttgt 14161 tgttgatccc gcccccgacc tcttgcttgt agccgtcccc gaactgcgag gtgcgcaccc 14221 gataggtaat atcgggcgcc tccccgtgct gggtggccca ggtgaacgtt tcaatcgcca 14281 tggttacctc ccgttgatgg ctcgccagat agccccgcca ggctgaacgc cgcgcgcaat 14341 ggctgcctca gcttccgctt tggtggcctg ctggatgttc cgaccggcct gcgtggtgtc 14401 ttcttgtgtc gcggtgccgc cttcgccagc cgtttgtacg gagaccgaga cggggaagtt 14461 gtaggtgttt ccaccgcctc ccccgccacc ac //