LOCUS MF417967 13361 bp DNA linear ENV 27-FEB-2018 DEFINITION Uncultured Caudovirales phage clone 3S_16, partial genome. ACCESSION MF417967 VERSION MF417967.1 KEYWORDS ENV. SOURCE uncultured Caudovirales phage ORGANISM uncultured Caudovirales phage Viruses; dsDNA viruses, no RNA stage; Caudovirales; environmental samples. REFERENCE 1 (bases 1 to 13361) AUTHORS van Zyl,L.J., Abrahams,Y., Stander,E.A., Kirby,B.M., Clavaud,C., Farcet,C., Breton,L. and Trindade,M.I. TITLE Novel phages from South African skin metaviromes JOURNAL Unpublished REFERENCE 2 (bases 1 to 13361) AUTHORS van Zyl,L.J., Abrahams,Y., Stander,E.A., Kirby,B.M., Clavaud,C., Farcet,C., Breton,L. and Trindade,M.I. TITLE Direct Submission JOURNAL Submitted (29-JUN-2017) Biotechnology, University of the Western Cape, IMBM, C2, Life Science Building, Robert Sobukwe Rd, Cape Town, Western Cape 7550, South Africa COMMENT ##Assembly-Data-START## Assembly Method :: CLC Genomics Workbench v. 7.5 Coverage :: 23x Sequencing Technology :: Illumina ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..13361 /organism="uncultured Caudovirales phage" /mol_type="genomic DNA" /isolation_source="healthy skin from scalp of human male" /db_xref="taxon:2100421" /clone="3S_16" /environmental_sample gene 529..756 /locus_tag="3S16_1" CDS 529..756 /locus_tag="3S16_1" /note="PHAGE_Strept_phiARI0468_1_NC_031929: hypothetical protein; PP_00002; phage(gi100038) 1.37e-19" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72662.1" /translation="MDLHHKGKMFLRAEVTEEQKETIKLLSDIKGITTQELLGQVVAN FVNNNRQLIEKYQNDLKVLVEDVSSNVNMNI" gene 768..1109 /locus_tag="3S16_2" CDS 768..1109 /locus_tag="3S16_2" /note="PHAGE_Strept_phiARI0460_1_NC_031913: hypothetical protein; PP_00003; phage(gi100041) 1.26e-78" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72673.1" /translation="MTQAERIREYYREHPAASYDEVAEVVGTTNSNVRANLAKDIKAG RCVRLEDKSYDYSPYFNHTQALTELVDWKNDTRREWVDMLTRAAEKETDSNVMRLLIK EANKLMKEVTK" gene 1109..1582 /locus_tag="3S16_3" CDS 1109..1582 /locus_tag="3S16_3" /note="PHAGE_Strept_Alq132_NC_013598: hypothetical protein; PP_00004; phage(gi273809759) 1.47e-67" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72680.1" /translation="MATLYELTGQFLDIYNLELDEETKLDTLDSIDWQTDYEEKVENY IKVMKNLEADVEARKAEIKRLTELNKADEKKKDHLKETLSTSMQLTGHERVDTPLFKV SFRKSQAVEVDETVLPESYKVATWKPDKKRLKEDLKNGLEIIGATLVERKNLSIR" gene 1579..2280 /locus_tag="3S16_4" CDS 1579..2280 /locus_tag="3S16_4" /note="PHAGE_Strept_YMC_2011_NC_018285: hypothetical protein; PP_00005; phage(gi399498699) 9.96e-157" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72681.1" /translation="MKITKATEITNNDACYLIYGNPGFGKTTAISFIPGKTLVINIDK SAKVLAGNPNIDIADVDTHKIWDEWLSVVKELLNGAGKPYDTIVVDNVSELFRACLAN LGRDGKNHRVPTQADYQRVDFTILDSLRALLQLNKRIVFTAWETSDQWSDENGMIYNR AMPDIRSKILNNFLGLTDVVARLVKKTTDDGEEVRGFILQPSASVYAKNRLDDRKGCK VDELFAQGLPEGTDN" gene 2237..3646 /locus_tag="3S16_5" CDS 2237..3646 /locus_tag="3S16_5" /note="PHAGE_Strept_858_NC_010353: orf37; PP_00006; phage(gi168229310) 0.0" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72682.1" /translation="MSFLLRDYQKELIIDVIKSMKAGNRKIMVQSPPRSGKTVVMSFI AKNATDKNKKVLFFSHRKEINEQVHETFKRGGVNLDNVIIGTVGSIVRKLNRLPEVDV ILVDEAHHIKAKQYQTILNHFTNATQLFFTGTPIRLDGSGFHDLADDLVVGKSIRWLQ EHGNIAEFDYYSINLLDMAKLKKRSGEFTNHSVDEALDFKTEYGDYIDHYERLAKGKQ AIVYTHSVEYAERVSKRFSEQGYQSGVVSGKTSQSEREILMQAFRDGKLTIMVNVNLF TEGIDLPNVDVCIMLRPTASLSLYLQFAMRALNPREGKKAILIDHVGNHIRHGLPNDD RDWTLDGTKKTKKTSERSTVTCEECFATFWRDQLEDGHCPYCNAEVIKKKTIEDIERE KSDVQLEKINQGMEFITIQGEKIEVKTEEAKVYRRVKTYGKRYTRCRNLSELKAFRLL NGYQPGWLWHKQKELNLWR" gene 3652..4134 /locus_tag="3S16_6" CDS 3652..4134 /locus_tag="3S16_6" /note="PHAGE_Strept_TP_778L_NC_022776: hypothetical protein; PP_00007; phage(gi557428136) 2.43e-80" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72683.1" /translation="MALFSVNYEAAEQFSSIEDGTYEVVVAQAEQSASQSGTDFLDIR LKIRDDFQQKFRNNLIFDKVWINKQTLQYPEWALQRYSKAVKIPEGVEVNTIEQFLGL ITGKTLKVTVKNEQSEYNGKTYDNLNIKKMEQSELPAYSGTVSSGPAQTKDDNLDLPF " gene 4139..4936 /locus_tag="3S16_7" CDS 4139..4936 /locus_tag="3S16_7" /note="PHAGE_Strept_YMC_2011_NC_018285: phage protein; PP_00008; phage(gi399498702) 3.67e-155" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72684.1" /translation="MIGMVDYALHYQKLGFSVIPIDKKSKRAITKFKDKTFTEDEIRR FWHEQPDANIAVRTTDFFVIDIDVSVTENGYESLKEWELSQYIPTTLTATTPSGGKHI FLKKPKGVELSQDIRVKPGIDIKANKNNYVLVAPSNSPKGKYVWDKTTDVIAEAPQEI VAILQTSKKAKEPLNFTTDYSRGEFSSKTAKLFEQVVFGLGDKGGRNNALASFIGGLL MRGVDVDAVYLLAKIANHYTSDSLPMDEVDRTFESMVRKEMDRRGGS" gene 4890..6476 /locus_tag="3S16_8" CDS 4890..6476 /locus_tag="3S16_8" /note="PHAGE_Strept_TP_778L_NC_022776: hypothetical protein; PP_00009; phage(gi557428138) 0.0" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72685.1" /translation="MKVWLERRWIDEVAVNIEAVKQEYKSKVIQHPAFIEKANDWREI RLACRNYRENWLENVKWNETQYGTREEKSNPPTRLTELAVAQGMEQILHIVNLPNERV AIYDPDHGYYHKDPSFAYKIIRLLEPNFSEVKSKNVLFMLASTPRLNQHEGFSCDFSI GAYKDPKRFILVKNGIYDKKEKKLQGFTHEFVAFSTIGTEYDHFAKSPVIDGWDIDSW LLDLMSGDEELVELIWQVISASLNGNYSYRKSIWFVGEGNDGKGTVQQLITNLVGMRN VASLKLNQFSERFALSMIEGKTVIIGDDVQAGIYVDESSNFNSVVTGEPVLVEEKNKQ PYTTVFRKTVIQSTNELPRFKNKTNGTYRRFAIIPFRKSFSSKEDNWAIKDDYIYRDE VLEYVLKKALEISFDRFIEPKASLEALEDFKESNDTVKAFVNEWFDKFQSTRLPSRFL WWLYQEWCRDEGVTKLTKRKFETQLAKNVPENWIKKKIKPLSQFIPSVDVPKHYLGFS WMDDESQILTSGYELVTVNR" gene 6728..7036 /locus_tag="3S16_9" CDS 6728..7036 /locus_tag="3S16_9" /note="PHAGE_Lactoc_98201_NC_031064: hypothetical protein; PP_00010; phage(gi100016) 4.45e-49" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72686.1" /translation="MKSEQEVQNEIRVALTQAGYTVFRTNVGKVKTADGRWFDTGLPK GHPDLYGFRSDGQIFYIEVKNENGRVRPEQENFIETVKKRGAIAGVARSAQEALELVR " gene 7033..7245 /locus_tag="3S16_10" CDS 7033..7245 /locus_tag="3S16_10" /note="PHAGE_Strept_phiNJ2_NC_019418: hypothetical protein; PP_00011; phage(gi414090244) 7.76e-05" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72663.1" /translation="MNNQFKVEVQCPFCGACYKRHAREGSKSIRCRFCGMSIFLRKTW KNNLDRIADEPFNQNEYVKDLNEVFE" gene 7242..7427 /locus_tag="3S16_11" CDS 7242..7427 /locus_tag="3S16_11" /note="hypothetical; PP_00012 N/A" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72664.1" /translation="MNIKQIMIDGLKRSIEQTEKEIEGYSKPCDKRVAQGRTAHREFL KKKLKKMKKQLKELEDE" gene 7420..8118 /locus_tag="3S16_12" CDS 7420..8118 /locus_tag="3S16_12" /note="PHAGE_Strept_phiARI0468_1_NC_031929: hypothetical protein; PP_00013; phage(gi100027) 2.79e-73" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72665.1" /translation="MNKQELIERIEGLKNIFGNKTKYIEKNTVIELVSKLDEPETGSA DEAPRYVKNVLARLRELPDHDREVWLKAIMGEFEQDFSRAKWREGYEQGKFEGMVKRE KVTIPQFVADWIEYCKVRGITLAHALYHSEEAKNKSVYRWLFEKSGRQETFAQAWIFG YEVEEEKRYLVKVKGICGNHETLNREKHSNKWLFSDREENSLYGTHHTRKELEDANFG WVFDCEGIEIEEVE" gene 8118..8279 /locus_tag="3S16_13" CDS 8118..8279 /locus_tag="3S16_13" /note="PHAGE_Strept_K13_NC_024357: phage protein; PP_00014; phage(gi658307263) 1.18e-24" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72666.1" /translation="MNEQNILETQLILGKQVLEIVFDLLKSDSKTGVVLPLNINDHGF TITVEKEVE" gene 8279..8464 /locus_tag="3S16_14" CDS 8279..8464 /locus_tag="3S16_14" /note="hypothetical; PP_00015 N/A" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72667.1" /translation="MERPERYPSGYGYFIPELIEDEDIIFKKNSEYHKQKKKEKKNPI LKEISPKIDGRFEEITE" gene 8461..8631 /locus_tag="3S16_15" CDS 8461..8631 /locus_tag="3S16_15" /note="hypothetical; PP_00016 N/A" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72668.1" /translation="MKDTKDFILAINNLKIDVITNSDNLNSYELGKIKRNAGDLYETL VWLQYAAEENEN" gene 8628..8888 /locus_tag="3S16_16" CDS 8628..8888 /locus_tag="3S16_16" /note="PHAGE_Strept_EJ_1_NC_005294: hypothetical protein; PP_00017; phage(gi39653703) 3.02e-48" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72669.1" /translation="MKRFIAIWILLSAGLNIWQMGRIAELEKKRPMVIYKADNQGAEI FGRVVEKGRHGKLYTITIRDYGIFVVTKDVYGKVKVGDEVRI" gene 8885..9559 /locus_tag="3S16_17" CDS 8885..9559 /locus_tag="3S16_17" /note="PHAGE_Strept_SpSL1_NC_027396: N-6 adenine-specific DNA methylase; PP_00018; phage(gi849256077) 8.48e-160" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72670.1" /translation="MKLFLNEDCMDVMKRYPDNYFDLAIVDPPYFSGPEKRKFYGRKV SPIGVSRLYGETSEWQIPNRDYFDELFRVSKNQIIWGVNYFDYSFGSGRIVWDKVNGQ SSFSDCEIAYCSLHDSTRMFRYMWNGMMQGKSISEGHIQQGNKALNEVRIHPTQKPIN LYLWLLQTYAKDGDKILDTHVGSASSLIACQELGFEYVGCELDRDIFNLAKQRLDVYE KKIKLF" gene 9568..10056 /locus_tag="3S16_18" CDS 9568..10056 /locus_tag="3S16_18" /note="PHAGE_Strept_EJ_1_NC_005294: DNA-binding protein; PP_00019; phage(gi39653705) 8.47e-71" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72671.1" /translation="MTTIDKVKQWFIERDLENGGRLDKQSLKLSEEFGELCAGYLKKN EKLTKDSIGDCAVVIVGLALLIKEDVHKIFDEVFSDEYYAKECLVFLNRTISNIQLSN GFTDKDLYIVNLTRSIYWLKSISYALGYNFEECFELAYQEIKDRKGRWIDGSFVKEED LT" gene 10053..10451 /locus_tag="3S16_19" CDS 10053..10451 /locus_tag="3S16_19" /note="PHAGE_Strept_MM1_NC_003050: hypothetical protein; PP_00020; phage(gi15088767) 3.55e-23" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72672.1" /translation="MIPKFRAWDTTNKEMFKDTFAITESGQVVVVDQSSVFVSPDYVF VDNLVIMQSTGLKDKNGQEIFEGDVVRQVRTQPTIENETITGVVTMLEGAWLIVNDNE QLASDLWSETDENEIIGNIFENKELLEDKE" gene 10448..10792 /locus_tag="3S16_20" CDS 10448..10792 /locus_tag="3S16_20" /note="hypothetical; PP_00021 N/A" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72674.1" /translation="MRPKRHPFSGAKKESEAKKISLMLKKVDESDLKGSVWAEPLHIY RKTRVHVEIEGYGKKIITEFKTDDMDFSRKASFFKRALFKRAEMMSQFDFRETTTEEW NRIILKLSEAIK" gene 10863..11051 /locus_tag="3S16_21" CDS 10863..11051 /locus_tag="3S16_21" /note="PHAGE_Strept_SM1_NC_004996: gp29; PP_00022; phage(gi32469460) 8.51e-30" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72675.1" /translation="MIDNFIGNLPGKAAWCWGNAIKYLLRFQKKNGLEDLKKARKNLD WLIEEIEHGQEQSRVRSV" gene 11017..11211 /locus_tag="3S16_22" CDS 11017..11211 /locus_tag="3S16_22" /note="hypothetical; PP_00023 N/A" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72676.1" /translation="MGKNNREYALYDGETFITCGTIKQICQETGLKKGTLSFYKSQKY RDRLKNPNEGMILIEIEEDE" gene 11208..11633 /locus_tag="3S16_23" CDS 11208..11633 /locus_tag="3S16_23" /note="PHAGE_Strept_K13_NC_024357: phage protein; PP_00024; phage(gi658307259) 1.31e-05" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72677.1" /translation="MTINIKQRLKALQYIDIKAKSKHQEIISLKSGILRGQQFDNMPK SKSNTNHSEELNISIIVKSDELYREIQSLYKERDELVRAIESLDDPVENIVMRLLYID GLSWKQVERKLRCSPATIQRARDKAIAKMVKIFDNNDSK" gene 12349..12489 /locus_tag="3S16_24" CDS 12349..12489 /locus_tag="3S16_24" /note="hypothetical; PP_00025 N/A" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72678.1" /translation="MIFIFNAIQNRKKLKKQKSSPVANESVLRQLDCLLKSRKDESKV KE" gene 12924..13259 /locus_tag="3S16_25" CDS 12924..13259 /locus_tag="3S16_25" /note="PHAGE_Strept_315.2_NC_004585: hypothetical protein; PP_00026; phage(gi28876232) 4.12e-56" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72679.1" /translation="MKPKRLVILNGRRTAVDYDKRNQEYTDYNRTRWKYDREVKQFYN STIWKRTSKQVLLESDYICAMCGNEATMTDHIISVKQDWSKRLDRSNLQASCKKCNDK KAIKEKNSF" ORIGIN 1 gtatacggta acgctagggt atacggcaac gctgaggtat ccggcaacgc tagggtatac 61 ggcaacgcta gggtatacgg caacgctagg gtatacggca acgcttgggt atccggcgac 121 gcttgggtat ccggcaacgc tgaggtatcc ggcaacgcta gggtatccgg caacgctgat 181 tatattgttt ttaaaaacac atggtctagc ggtcgatatt ttacttacac aaaatctaac 241 aaaaaatgga gagttggttg tttttacggc aacggctatg aattgattga aaaagcatat 301 aaagatagcc aaaaatcagg ggatttttac aaagcgtatg tcgaatttgt cgaaaagcta 361 gaagaaatcg aaaaaatcca caaggagcaa tgatatgtat acatgggatt gtggatgtag 421 agattgtgga aatacattcg aatacatcga tagttatcca atcgttgaat gcccaaaatg 481 tgggagcgat gatctaaaga atgaatttaa aggaagggag tatgactaat ggatttgcac 541 cataaaggaa aaatgtttct tagagcagaa gtgactgaag aacagaaaga aactatcaaa 601 ttattatctg acataaaagg tataacaaca caagaattac ttggacaagt agttgcaaat 661 tttgttaata acaatagaca actaattgaa aaataccaaa acgatttgaa agtgttggtt 721 gaagatgtaa gttcaaatgt gaatatgaat atttaaggag aagaaccatg acacaagcgg 781 aacgaattag ggaatattat agagagcacc ctgctgcctc atatgatgaa gtggctgagg 841 ttgtcggtac aacaaatagt aatgtaagag cgaacttagc caaagacatc aaggcgggca 901 gatgcgttcg cttggaagac aagtcatatg actattcacc ttacttcaac cacacacaag 961 cactcaccga gttggttgat tggaagaatg atactagacg tgagtgggtg gatatgctga 1021 caagagcagc agaaaaagaa acggatagca atgttatgcg tttgttaatc aaagaagcaa 1081 ataaattgat gaaagaggtg acgaaataat ggcaacactt tacgaactaa caggacaatt 1141 ccttgatatc tacaacttgg aattggacga agaaactaaa ctagatacgc ttgatagtat 1201 cgactggcaa actgactatg aagaaaaagt cgaaaattat atcaaggtta tgaagaacct 1261 tgaagcagat gttgaagcac gtaaagcaga aattaaacgc ttgactgaat tgaacaaggc 1321 tgatgaaaag aagaaagatc acttgaaaga aacactttct acaagcatgc agttaactgg 1381 tcatgaacgt gtagacacac ctttatttaa ggtttcattt cgcaaatctc aagcggttga 1441 agtagatgaa acggtcttgc cagaaagcta taaagtagct acttggaagc ctgacaagaa 1501 acgcttaaaa gaggatttga agaatggtct tgaaattatc ggtgcaactc tagtagaaag 1561 gaagaatttg agtataagat gaaaatcact aaagcaacag aaattacaaa taacgatgcc 1621 tgttacctga tttatgggaa tccaggtttc gggaaaacaa ctgcaatttc attcattcca 1681 gggaagacat tggttatcaa cattgataag tcagcaaaag tcttagctgg caatcctaac 1741 atcgatattg cagatgttga cacacataag atttgggatg aatggttatc agtggttaaa 1801 gaactactga atggagcagg taaaccatac gatacaatcg tggttgataa tgtttctgaa 1861 ttattcagag cctgccttgc caatcttgga cgcgatggga aaaaccatcg agtaccaaca 1921 caagcggatt accaaagggt tgacttcact atcttggata gtttacgagc gcttttgcag 1981 ttaaacaaac ggattgtatt tactgcatgg gaaacatcag accaatggtc agacgagaat 2041 ggcatgattt acaacagggc aatgccagac attcggagta aaatcctaaa taacttcctc 2101 ggtttgacag atgtggtcgc tcgtctagtt aagaagacaa cagatgacgg tgaggaagtc 2161 cgtgggttta tcctacaacc ttctgcaagc gtatatgcta agaaccgttt ggacgatagg 2221 aaggggtgta aggtagatga gctttttgct cagggattac cagaaggaac tgataattga 2281 cgttatcaaa tccatgaagg caggcaatcg aaagataatg gtgcaatccc caccacgttc 2341 aggaaagacg gtcgttatgt ctttcatcgc taaaaatgca actgataaaa ataaaaaagt 2401 tctatttttt agccatagga aagaaatcaa tgagcaagtc cacgaaacat tcaaacgtgg 2461 aggagtgaac ctagacaacg ttattattgg aacagttgga agtattgtac gtaaattgaa 2521 tagactgcct gaggtagatg taatccttgt agatgaagct caccacatta aagcgaaaca 2581 atatcaaacg attttaaatc acttcacaaa cgcaactcaa ttattcttta caggaactcc 2641 aatccgatta gatggctctg ggtttcatga tctagcagat gatttagtag tcggtaaatc 2701 catccgatgg cttcaagaac acggaaatat agcggaattc gattactatt caataaatct 2761 attagacatg gcaaaactaa aaaaacggtc tggagaattt actaaccact cagtcgatga 2821 agcacttgat tttaaaacag aatatggtga ttatatcgac cactacgaac gattggcaaa 2881 aggaaaacaa gctatcgtct atacccatag cgtagaatac gccgagaggg tttctaagcg 2941 attttctgag caaggctatc aatcaggtgt agtcagtgga aaaacctcac agagcgaacg 3001 tgagatcctt atgcaagcat tcagagatgg taagttaaca attatggtta atgtcaatct 3061 tttcacagaa ggtattgacc taccaaacgt agatgtttgt atcatgttac gacctactgc 3121 atcactttcc ttgtatcttc aatttgctat gagggcatta aacccaagag aaggaaagaa 3181 agcaatcttg atagatcacg taggtaacca tattagacat ggactaccaa acgatgatag 3241 agattggact cttgatggca caaagaaaac aaagaagaca tctgagagat caacagtaac 3301 ttgtgaggag tgttttgcaa cgttttggag agaccaatta gaagacggtc attgtccata 3361 ttgcaatgcg gaagtaatta aaaagaaaac gattgaggat attgaacgtg agaaatcaga 3421 tgttcaatta gaaaaaatca atcaaggaat ggaattcatt accattcaag gcgaaaaaat 3481 agaggtcaag acagaagaag cgaaagtgta tcgtcgcgtc aagacctatg gtaaacgata 3541 cacaagatgt cgaaatttgt cggaattaaa agcattccga ttactcaatg gctatcaacc 3601 agggtggttg tggcacaagc aaaaagaatt aaatttatgg agataataaa catggcactt 3661 ttttcagtaa attatgaagc agcagaacaa ttttcatcta tcgaagacgg aacatatgaa 3721 gtagtagtag ctcaagcgga gcaatcagca agtcaaagtg gaacagactt tttagacatt 3781 cgcttaaaaa tccgtgatga cttccaacag aaattccgta acaacctaat ctttgacaaa 3841 gtatggatca ataaacaaac ccttcaatat ccagagtggg ctttgcaacg atattctaaa 3901 gcggttaaaa ttcctgaagg tgtggaagta aatacaattg aacaattctt aggtcttatc 3961 actggtaaaa cgttgaaagt gactgtaaaa aacgaacagt cggaatataa cggcaagacc 4021 tacgataact tgaatatcaa gaaaatggaa caatctgaat taccagcata ctctggaacg 4081 gtatcatctg ggccagcgca aactaaagat gataatttag acttgccatt ctaagcctat 4141 gattgggatg gtagattacg cccttcatta tcaaaaatta ggtttctcag tcatcccaat 4201 cgataagaaa agtaaacgtg caatcactaa attcaaagat aaaacattta ctgaggatga 4261 gattagaaga ttttggcatg aacaaccaga tgcaaatatt gcagtaagaa caaccgattt 4321 ctttgtgatt gatattgatg tatcagtcac agagaatggt tatgaatctt taaaagagtg 4381 ggagttatca cagtatattc ctactacctt gactgctaca acccctagtg gtggaaagca 4441 tatttttctt aaaaaaccaa aaggggttga gttaagtcaa gatattcgtg tgaaacctgg 4501 cattgatata aaggcgaata aaaacaatta tgtattagtc gcaccaagca atagtccaaa 4561 agggaaatat gtttgggata aaacaacaga tgtgattgct gaagcaccac aagaaatagt 4621 tgcaatccta caaacctcta aaaaagcaaa agaaccactt aacttcacaa ccgattacag 4681 tcgaggagaa ttctcaagca agactgcaaa attattcgag caagttgttt ttggtttagg 4741 tgacaagggt ggtagaaata acgctctcgc tagttttatc ggtggcctct taatgcgagg 4801 ggtggatgta gatgcagttt atttacttgc aaaaatagca aatcactata cttcagacag 4861 tttaccaatg gatgaagtag atagaacatt tgaaagtatg gttagaaagg agatggatag 4921 acgaggtggc agttaacatt gaagcagtga aacaagaata caaaagtaaa gttatacaac 4981 atccagcttt catcgagaaa gcgaacgact ggagagaaat tcgattagct tgtcgaaatt 5041 atagagagaa ttggctcgaa aatgtaaagt ggaacgaaac acagtatggt acacgagaag 5101 aaaaaagcaa tccacctaca cgtttgactg aattagcagt agcacaaggc atggaacaga 5161 ttttacatat cgtaaactta ccgaatgaac gtgtggcaat ttatgatcca gaccatgggt 5221 attatcataa agaccctagc tttgcttata aaattatccg attacttgaa ccgaatttta 5281 gtgaagttaa gtcaaaaaac gttctgttca tgcttgcatc tactccaaga ttaaatcaac 5341 atgagggttt ttcatgcgat ttctcaattg gtgcatataa agaccctaaa cgttttatct 5401 tagtcaaaaa tggtatttat gacaaaaaag agaagaagtt acaaggattt acgcatgagt 5461 ttgttgcatt ctcgaccatt ggtaccgaat acgaccactt tgcaaaatct cctgtcatag 5521 acggttggga tattgatagt tggttactcg accttatgag tggagatgaa gagcttgtag 5581 aactcatctg gcaagtcatc tcagcaagtt taaatggtaa ttactcatat cgtaaatcaa 5641 tctggtttgt cggtgaaggt aatgatggta agggtactgt ccaacaactc atcactaacc 5701 tagttggtat gcgaaacgtg gccagcttaa aacttaatca attttcagag cgattcgctc 5761 tatcgatgat tgaaggtaag actgtcatta tcggagacga tgtgcaggct ggtatctatg 5821 tagacgagtc ttcaaacttc aactcggttg taactggtga gccagtcttg gttgaagaaa 5881 aaaataaaca accttataca actgttttta gaaagacggt tatccagtct acaaatgaat 5941 taccacggtt taaaaacaaa acaaatggaa catacagacg atttgcaatc atacctttca 6001 gaaagtcgtt ttcgagtaag gaagataatt gggcaatcaa agatgattat atctatcgtg 6061 atgaagtttt ggaatacgtc ttgaagaaag ctcttgaaat ttcattcgat agatttattg 6121 aacctaaagc atcacttgaa gccttagaag atttcaaaga atcaaatgat accgtcaagg 6181 cattcgtcaa tgaatggttt gacaaattcc aatccacgcg cctaccttca cggtttttgt 6241 ggtggttgta tcaggaatgg tgcagagatg agggagtcac aaaattaaca aaacgcaaat 6301 ttgaaactca attagcaaaa aacgtacctg agaattggat caagaaaaaa attaaacctt 6361 taagccaatt catcccttcg gtagatgttc caaaacatta tctaggtttt tcttggatgg 6421 atgatgaaag tcaaatactt acatcagggt atgaattagt taccgttaac cgttaggtta 6481 ccgtatgttt ttatactacg gtaaccatgt ttaagcctta tgtatcaagg ggtttgctta 6541 tgttggttac cgtattacct ttcttttcta ttgaaataat aaaaaaataa ataatataaa 6601 tataaataaa gagaaaaggt aacggtaacg gtaacctgag aggtaaaaaa cggcgcaaac 6661 tattgatatg actgaatttt tgttggttac cgttctaaaa tgaagtacgg taacctgagg 6721 aggtattttg aagtcagagc aagaagtaca aaatgaaatt agggtcgcat tgactcaagc 6781 tggatatacc gtatttcgaa cgaacgttgg aaaagttaag accgcagacg gcagatggtt 6841 tgatactgga ttaccaaaag ggcatccaga cctatatggt tttagatcgg atggacagat 6901 tttttatatc gaagttaaga atgaaaatgg tcgtgtgaga ccggaacagg aaaattttat 6961 tgagacagtc aaaaaacgag gtgcgatagc tggtgttgct agaagtgctc aggaagcgtt 7021 ggagttagtc agatgaataa tcaatttaaa gtagaagtac aatgtccatt ttgtggagcg 7081 tgttataaaa ggcacgctag agaaggttct aaatcgatta gatgtagatt ttgtggaatg 7141 tctatttttt taaggaagac atggaagaac aatttagaca gaatcgctga tgaaccattc 7201 aaccaaaatg aatatgtcaa ggatttgaat gaggtgtttg aatgaatatc aaacaaataa 7261 tgattgacgg cttgaagcgg tcaatagagc aaacggaaaa ggaaatcgag gggtattcga 7321 agccttgcga taaacgagtg gcacaagggc gtactgctca tcgtgagttt ttgaagaaga 7381 aactaaagaa aatgaaaaaa cagttgaagg agttggaaga tgaataagca ggaattgatt 7441 gaacggatag aaggcttaaa aaatattttt ggcaacaaaa caaaatacat tgagaaaaac 7501 acagtaatag aacttgtttc taaactagac gaaccagaaa cagggagcgc agacgaagct 7561 ccacggtatg ttaaaaacgt actagcacga ttgcgagaat tgccagacca tgatcgtgaa 7621 gtttggctaa aggccatcat gggtgaattt gaacaagatt tcagtcgtgc aaaatggcgt 7681 gagggttatg agcaaggcaa atttgaggga atggttaaac gtgagaaagt cacaatcccg 7741 cagtttgtgg cggattggat tgagtattgc aaagtcaggg gaattacttt agctcacgca 7801 ctctatcatt ctgaagaagc aaaaaacaaa agcgtttatc gttggctctt tgaaaaatca 7861 ggtaggcaag aaacattcgc tcaagcctgg attttcggct acgaggtcga ggaagaaaag 7921 cggtatttgg tgaaagtgaa aggcatttgt ggaaatcacg aaactttgaa ccgtgagaaa 7981 cattcaaaca aatggctttt ctcagaccgg gaagaaaact cactttatgg cactcaccac 8041 acccgcaaag aactagaaga tgccaacttt ggctgggtat tcgattgtga ggggattgag 8101 attgaggagg tggagtgatg aatgaacaaa acattttaga gacacaatta attttaggca 8161 agcaagtttt agaaattgta tttgatttgc taaaaagtga ctcaaaaaca ggggtagttt 8221 tgcctttaaa tataaatgat cacggattta caattacagt tgaaaaagag gtggagtgat 8281 ggaacgacct gaacgatacc catctggata tggatacttc attcctgaac ttattgaaga 8341 tgaagatatt atctttaaaa aaaatagcga atatcacaag cagaagaaga aagaaaagaa 8401 gaatcccatt ttaaaagaaa taagtccaaa aatagatggg cgctttgagg agataacaga 8461 atgaaagaca caaaggattt tattctagct atcaataatt taaaaattga tgttataaca 8521 aactcagata atctaaatag ctatgagtta ggaaagatca agagaaacgc aggagattta 8581 tatgaaactc ttgtatggtt gcagtatgct gcggaggaga atgaaaattg aaacgattca 8641 tcgcgatctg gatcctgcta tctgctggat tgaacatctg gcagatgggc aggattgcag 8701 agttagagaa aaagcgtccg atggttatct ataaagctga caatcaaggc gctgagatat 8761 ttggacgtgt cgtcgaaaaa ggacgacatg gcaagttgta cacaattacg attcgtgatt 8821 acggcatttt cgtagtcacg aaggatgtgt atggcaaggt gaaagttgga gatgaggtga 8881 ggatttgaaa ttatttctta acgaagattg catggacgtc atgaaaagat atcctgacaa 8941 ctattttgat ttagctattg ttgatccacc gtatttttcc gggccagaaa aaagaaaatt 9001 ttacggacga aaagtcagtc caataggtgt aagcagactg tatggcgaaa cctcagagtg 9061 gcaaattcca aacagagatt attttgatga acttttcagg gtatctaaaa atcaaattat 9121 ttggggtgtg aactacttcg actattcttt tggttctggc cgtatcgttt gggacaaagt 9181 taatggccag tcaagtttct cagattgtga gatagcgtac tgcagtttac atgatagtac 9241 acggatgttt cgctatatgt ggaacggtat gatgcaaggg aagtcaatat ctgaaggaca 9301 tatccagcaa ggaaacaagg ctttgaatga ggttagaatt catccaactc aaaaacccat 9361 caatctttat ctttggttat tgcaaactta cgcaaaagac ggagacaaga ttcttgatac 9421 tcatgttggt tcagcaagta gtttaatcgc ttgtcaagaa ttaggttttg agtatgtagg 9481 ttgcgagtta gatagagaca tctttaatct tgctaaacag agacttgatg tttacgagaa 9541 gaaaataaaa ttattttagg agttaaaatg acaacaatag acaaagtcaa gcaatggttt 9601 attgagcgtg acttagaaaa cggtggacgg ttagacaaac agtcattaaa gctcagtgaa 9661 gaattcggtg agctatgtgc aggctatctc aagaagaatg agaagctgac aaaggacagc 9721 atcggagact gtgcggtcgt gattgtcggt ctagccttgc ttataaaaga ggatgtgcat 9781 aagatttttg atgaagtttt tagcgacgaa tactatgcca aggaatgtct ggtctttttg 9841 aataggacta tcagcaatat tcagttatca aatggattca cggataaaga tctatatata 9901 gtcaatttaa ctcgttcaat ttattggtta aaatcaatca gctatgcact tggttataac 9961 ttcgaagaat gttttgaact agcttatcaa gaaatcaaag accgcaaggg tcgttggatt 10021 gacggaagtt tcgtgaaaga ggaggatttg acatgatacc aaaatttaga gcgtgggata 10081 ccacgaataa agagatgttt aaagatactt tcgcaataac agaaagcgga caggttgtag 10141 tagttgacca atcctctgtc tttgttagtc cagattatgt tttcgttgac aacctagtca 10201 tcatgcagtc aacaggactc aaagataaga acggtcaaga aatctttgag ggggatgtag 10261 ttagacaagt acgaactcag ccaacaatag aaaatgaaac aattacaggg gttgtaacca 10321 tgcttgaggg cgcttggttg attgtgaatg ataatgagca attagcaagt gatttgtggt 10381 cagaaactga cgagaacgaa atcatcggca atatctttga aaacaaagag cttttggagg 10441 ataaggaatg agaccgaaaa gacacccttt cagtggtgct aaaaaagaga gcgaagctaa 10501 gaaaatatct ttaatgctta aaaaagtcga tgaatctgac ttgaaaggaa gtgtttgggc 10561 ggagcctcta catatctata gaaaaacaag agtccatgta gagatagagg gttatggaaa 10621 gaaaatcata accgaattta aaacagatga tatggatttt tccagaaaag cttcattctt 10681 taagagggca ttattcaaaa gagctgaaat gatgtctcag ttcgatttta gagaaacgac 10741 aactgaggaa tggaatcgaa taatcttaaa actttcagag gctatcaaat gaaacctgaa 10801 aaattgacaa cataaacaaa ccaagctatt atcaaggctc aaaaggtctt gaaagtattg 10861 aagtgattga taacttcatt ggcaacttgc caggtaaggc agcatggtgc tggggcaatg 10921 caatcaagta tctattgcgt tttcaaaaga aaaacggtct tgaggatttg aaaaaagcac 10981 gcaagaacct tgattggtta attgaggaga tagaacatgg gcaagaacaa tcgagagtac 11041 gctctgtatg atggtgaaac attcatcact tgcgggacga ttaagcaaat ctgtcaagag 11101 actggcttaa agaaaggaac cttgtcgttt tacaagtcac aaaagtacag agacagactt 11161 aaaaatccaa acgaaggaat gatactgatt gaaatcgagg aggatgaatg acgataaata 11221 tcaaacaacg gttgaaagca ttacaataca ttgatatcaa agcgaagtca aagcatcaag 11281 aaattatcag cttaaagtca ggtattttga gagggcagca gtttgacaac atgccgaagt 11341 caaaaagcaa tacgaaccac tctgaggaat tgaatatctc aattatcgtt aagtctgacg 11401 aattgtatag agaaattcaa tctctataca aagagcgtga tgaacttgta agagctattg 11461 agtcgctcga tgatccagta gagaacattg tcatgcgact actctatatc gacggactat 11521 cgtggaaaca ggtcgaaaga aagctgcggt gcagccctgc aactatccag agagcgagag 11581 ataaagcgat agctaaaatg gttaaaatat ttgataataa cgatagtaaa tgataattgc 11641 aaagtgatat tattgtattg tcagcaagta cggtaaagcg gactgatggc tcctttaata 11701 attttttgta acggtatcag ggacgagcca gtgatttcct cttagtcttt cgtttagtct 11761 ctgatgtcgt tattttttta aacttgaaaa tggttgcgga gcgactggac cttgcatgat 11821 tgcgtagcta attatattcc ggataagtta tgagctagag ggtttgattc cctcagaggt 11881 cttaaagact acaaaaaaga aataagtaat ttctaattaa caagcaaggt tgtagtcgcc 11941 ttgcattttt agggcttagc ctagataatc tgtggtaact caggaaaagg atgtttttaa 12001 tctatcaaac atcctgccag taatggtcaa tctaagcaat gcaatcttaa ctatttcagt 12061 tctggaatag gttggcgaag ttaaagcaga gagattccaa cggcaaggtg ctgaggaaac 12121 gcaaacgtgg cagtttggct gtgagacgag cctataagag gaaaaaggta tttggttcga 12181 ggtgcaacaa gagcttaata ccatatctta caaaaattgg gtgcctccca aaagtatgta 12241 aggtgagttg attgtccgca aaacaatcga taacaagcag gcgctgtgca ttttgttctt 12301 caaaagagaa tgaaacacat ggcgatgcgt gtctgtgata gataaaagat gatttttata 12361 tttaatgcta ttcaaaatag aaaaaaactc aaaaagcaaa agtcatcgcc cgtcgcaaat 12421 gaaagtgtac ttcggcaatt agattgcctg ctcaagtctc gcaaggatga gagtaaagtc 12481 aaagagtaaa gcagcttaga cttttagcgg ggtcttcgtt aattgaaaaa tggcttagta 12541 gtttgtgatg taaggagtga ttggcctaac caatcgtgca tgagtgatac aagtaggaat 12601 acttgtggac aagataataa actataagtt atcaaaagtc actcgtttaa agcagtagtc 12661 tcatgctagt taatggatat atggtagacg gattaagtcc tgtttaggga attgagacgc 12721 cacaggttcg agtcctgtcg ttccaattgt atctctgtga gtagctatca caataggggt 12781 acagggcggt aattagattt aggctgatta acctgtagga cagagataaa gtagcgctat 12841 ataaggctct ggtgggagag gcacccactt accgcataca gtcacacatt gtgtggcttt 12901 ttgatttata aggagaaatg ggaatgaaac caaaaaggct ggtaatttta aatggcagaa 12961 gaacagcggt cgattatgac aaacgtaatc aagaatacac agactataat cgtactcgtt 13021 ggaagtatga tagagaggtt aagcagttct ataactcaac tatctggaag cgaacaagta 13081 aacaagtatt actagagtct gattatatct gtgctatgtg tggcaatgaa gctacgatga 13141 ctgaccatat cattagtgtg aaacaagatt ggtcaaagag attagatcga agtaatcttc 13201 aagcaagttg taagaaatgt aatgataaga aagcaataaa ggagaaaaat tctttttgaa 13261 aataaataga aataacaaaa aataagatgg aatatcacaa gggaatgcac tgcaaaacat 13321 acggaaatac cccctatcgt ttagaacggg ggtaggtatt g //