LOCUS MF417972 13657 bp DNA linear ENV 27-FEB-2018 DEFINITION Uncultured Caudovirales phage clone 10S_4, partial genome. ACCESSION MF417972 VERSION MF417972.1 KEYWORDS ENV. SOURCE uncultured Caudovirales phage ORGANISM uncultured Caudovirales phage Viruses; dsDNA viruses, no RNA stage; Caudovirales; environmental samples. REFERENCE 1 (bases 1 to 13657) AUTHORS van Zyl,L.J., Abrahams,Y., Stander,E.A., Kirby,B.M., Clavaud,C., Farcet,C., Breton,L. and Trindade,M.I. TITLE Novel phages from South African skin metaviromes JOURNAL Unpublished REFERENCE 2 (bases 1 to 13657) AUTHORS van Zyl,L.J., Abrahams,Y., Stander,E.A., Kirby,B.M., Clavaud,C., Farcet,C., Breton,L. and Trindade,M.I. TITLE Direct Submission JOURNAL Submitted (29-JUN-2017) Biotechnology, University of the Western Cape, IMBM, C2, Life Science Building, Robert Sobukwe Rd, Cape Town, Western Cape 7550, South Africa FEATURES Location/Qualifiers source 1..13657 /organism="uncultured Caudovirales phage" /mol_type="genomic DNA" /isolation_source="healthy skin from scalp of human male" /db_xref="taxon:2100421" /clone="10S_4" /environmental_sample gene complement(<1..781) /locus_tag="10S4_1" CDS complement(<1..781) /locus_tag="10S4_1" /note="Similar to YP_950680.1 [Staphylococcus phage PH15] (BLASTp score : 424). Similar to PF06605.6 [Prophage_tail] (hmmscan score : 199.0)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72757.1" /translation="MAIALKTLQGREYPLQVETKLNEKLNEDGSLEFEIVENNATFDA IGSITKMWTVSNIGGDGDPREYRIVMLDKTTVGEKMKISVKARPVELDDLNNTRIYET YNGSFTGKEYFDLVFKSTGYKYILNKKVESSKFENLGNHETNLELFKKGLERYDLEYE YDANTKTFSLFDSVEHKADYYIKAGVNANNVKIQEDASKCYTYIKGFGGFDDQQTYNE ASLQYEYTHPLADLVGKRHAPPVIDGRMTKPDTLYTSSHGAS" gene complement(794..1732) /locus_tag="10S4_2" CDS complement(794..1732) /locus_tag="10S4_2" /note="Similar to YP_950616.1 [Staphylococcus phage CNPH82] (BLASTp score : 457). Similar to PF05709.6 [Sipho_tail] (hmmscan score : 121.6)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72758.1" /translation="MILHDIEIIKDKESNFISDNRYTHQALRVISYDVQGAGYERKFD NIDRVNGRFHNSTTEEKKKVTIKLFYSVEKIAYASHLKARLQALLRGELYLRELAVPE AKIKFESPFAPQKQEFELEYVDGRQIKVGLVNDISFDTTQTSGEFTLEFETLELPYFE SIAYNTILEKEDGNLEKWGIPDDNPFDVPDQERRSTFYDCKTGVVYYGGTVEIKQFNQ DSVVELTLGENISKDDKAGITFYLQYSDRMKIAGLEMRAGDVIKFDGLHTYRNNIRID EYNTTKQQPVLYPGWNSFNSTKTLQKVVFKHKRYYM" gene complement(1748..4831) /locus_tag="10S4_3" CDS complement(1748..4831) /locus_tag="10S4_3" /note="Similar to YP_006560957.1 [Staphylococcus phage vB_SepiS-phiIPLA5] (BLASTp score : 1292). Similar to PB002824 [Pfam-B_2824] (hmmscan score : 669.2)." /codon_start=1 /transl_table=11 /product="putative tail tapemeasure protein" /protein_id="ASN72759.1" /translation="MNKNFVARVSAIINNFQRNIRKAQQMAKTSIPDEIETEVTANIS RFQRALNRAKAMAQRWREHKVEIDANATPIQRVIATIKGKLNAIRDKKVDIDGDASPL NRVVTAAKARLAIWRKHTVKLDVDTTGMTKLQSAMAIAKRSLDEYQTKMDSIASNIRT FGTIFSQQVKGLMIASIQALIPVIASLVPAIMAVLNAVGVLGGGVIGLAGAFGIAGAG AMAFGIMATSAIKMLNDGTLQATAETRRYQSALSQVQSTWQGIIKQNQAQIFNTLANG LQTVNVALQRMTPFLSGVAKGMEQASAGMLKWAQHSQTASKFFNMMNTTGVKTFNTLL SAAGRFGDGLVNVFTQLAPLFLWTAQQLNKLGASFQKWANSAAGQNAIRSFIEYTKTN LPKIGQIFGNVFAGIGNLMAAFAQNSAGIFDWLVKLTGQFRAWSEQVGKSEGFKKFVQ YVQQNGPVIGQLIGNIVRALIAFGTAMAPIASVILKFVTALAGFIAKLFETHPAIARM VGVMMILGGIFWALMAPVIAISSVLSNVFGMSVVQVITKIAQLAKSGQLLSTIFEMLA SPFKFLASLLPMLGEAFAAVGTAIAGISAPVWIVIGVILALVGIITYLWKTNEGFRQM ITETWNGIVTAISGAISSIINWFMQLWASIQQTLQPIMPILQMLGQVFMQVLGVLVMG IIQALILTFQALWTAVSVIFTAIGGIISVVVQIIVGLFTALIQLLTGDFSGAWLTLQN MVSNVMNTIWNTLVSIWSQISNFIFSVLNRILGTNITSWSQIWSAITDFVTRIWNSVS NWFGRVVSTVGSKMVQAWNYVKSHGAQWVSSIWNTMTNFVHRVVSGFFQVVNSVGNGM RNAVNKVKSFAQSFISAGLDMMRGLVNGIKQGMAWVVSAAKNAASSALHAAKSVLGIH SPSREFMSVGMFTMQGFGIGIDKHVGTAVSSVKQASRKVMDAFQPELSSDLATGLSGG LNSDVNAHMSRDVRHSMQENNKPVVNVTVRNESDIPAIKSYIEDSNSKDASFDLY" gene complement(4834..5136) /locus_tag="10S4_4" CDS complement(4834..5136) /locus_tag="10S4_4" /note="Similar to YP_950614.1 [Staphylococcus phage CNPH82] (BLASTp score : 123). Similar to PB003681 [Pfam-B_3681] (hmmscan score : 71.8)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72760.1" /translation="MSLTPNEWRDWIIGRRQALLDQQENMLFVAQANGLVQAGKSLKR LQKQIDHARYVVRGEEEEYERMRKRKLAQNKHNREIQKRGTRNFLDKMRNTSHKGG" gene complement(5199..5693) /locus_tag="10S4_5" CDS complement(5199..5693) /locus_tag="10S4_5" /note="Similar to YP_006561175.1 [Staphylococcus phage vB_SepiS-phiIPLA7] (BLASTp score : 266). Similar to PF12363.3 [DUF3647] (hmmscan score : 120.6)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72761.1" /translation="MTENTINPITTLNINGEEIEAKTVFAFDHKAKQFARDEQDENGK TTKVSGFNAIYNGLLERDTNAIADFWECATAYLGKNAPKREDIEMALSEVIEEKQDTI ELLQGALDKLNNSGFFKQKSRMFWTQMNSAPSMVKEDEKESTKNGIEFMKNNYKEIMG ELPY" gene complement(5755..6294) /locus_tag="10S4_6" CDS complement(5755..6294) /locus_tag="10S4_6" /note="Similar to YP_950675.1 [Staphylococcus phage PH15] (BLASTp score : 339)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72762.1" /translation="MAQNKYIAAIQIADKELASTLKEEDAILLASLAEGGHTISNDLA EIIQGGKKDYGRNSVEEEIKLTVDRVPGDKGQEALKEAVKNFKQLRIWIWEVKKREGF HHGTFAYVIVEEHEWSFDDEDDKIEITAKVKFNSADGKVSELPPEWLNPSAQAPTVEW EDMGAYTDTYENRTPKSGA" gene complement(6281..6718) /locus_tag="10S4_7" CDS complement(6281..6718) /locus_tag="10S4_7" /note="Similar to YP_950611.1 [Staphylococcus phage CNPH82] (BLASTp score : 271). Similar to PF11367.3 [DUF3168] (hmmscan score : 67.6)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72763.1" /translation="MWVSVERYLFNEIYNKLKSNPIINKQLGGRVFDCVQKDAVYPYI VVGETNVTNDETTTSMVEDVGITFHVYSQAYNRNEASQIIQFLGYVLNSEIEINNYSF IKSRIDTQEVITDIDQYTKHGIIRLVFKYRHNIRKRSVTNGSE" gene complement(6731..7144) /locus_tag="10S4_8" CDS complement(6731..7144) /locus_tag="10S4_8" /note="putative ORF040; Similar to YP_240082.1 [Staphylococcus phage 37] (BLASTp score : 226). Similar to PF04883.7 [DUF646] (hmmscan score : 66.4)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72764.1" /translation="MAKVKYGNWDLVKELEDYEKEMERWVKKGIAKTTLKIYNTAVEM APVDMGYLKESIDFMYKDGGFTGIISVGAEYAIYVEYGTGIYAEGPGGSRATKLPWSY KDADGDWHTTYGSPAQPFWNPAIDAGTQVFNKYFS" gene complement(7137..7466) /locus_tag="10S4_9" CDS complement(7137..7466) /locus_tag="10S4_9" /note="Similar to YP_950609.1 [Staphylococcus phage CNPH82] (BLASTp score : 190). Similar to PB011122 [Pfam-B_11122] (hmmscan score : 172.0)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72765.1" /translation="MFNPYDEFPHVISIGVREVIGKYPNEKERFKSENTIKGFMDTPT SSEQLKYHQMSQDYDRNLYTPYNIPITTKTLFKYQGKTYEVVGEPVDQGGQNEINLTR LRECPIG" gene complement(7459..7773) /locus_tag="10S4_10" CDS complement(7459..7773) /locus_tag="10S4_10" /note="Similar to YP_006560950.1 [Staphylococcus phage vB_SepiS-phiIPLA5] (BLASTp score : 154). Similar to PF05135.8 [Phage_connect_1] (hmmscan score : 65.3)." /codon_start=1 /transl_table=11 /product="putative DNA packaging protein" /protein_id="ASN72766.1" /translation="MDAKDVKLLNNLSLDDTSQDDTLEMLIDKYLKVAEEYCNQTFDK ENIPSSVEKFIANCIQHGTTGNVSSRTMGTVSYSFVTDLPDETYSYLKPFRRLRWSGY HV" gene complement(7773..8057) /locus_tag="10S4_11" CDS complement(7773..8057) /locus_tag="10S4_11" /note="Similar to YP_006561169.1 [Staphylococcus phage vB_SepiS-phiIPLA7] (BLASTp score : 134). Similar to PF07498.7 [Rho_N] (hmmscan score : 38.5)." /codon_start=1 /transl_table=11 /product="putative rho termination protein" /protein_id="ASN72767.1" /translation="MYKVIEYFVDLQDNDREYNVGDTFNHDNVSEERLTELSTKNNRQ RKPLIERVEEQTKLSDMKVADLKELAKQRDIEGYTKMKKDELVEVLGSVE" gene complement(8074..8889) /locus_tag="10S4_12" CDS complement(8074..8889) /locus_tag="10S4_12" /note="Similar to YP_006560948.1 [Staphylococcus phage vB_SepiS-phiIPLA5] (BLASTp score : 520). Similar to PB001763 [Pfam-B_1763] (hmmscan score : 113.9)." /codon_start=1 /transl_table=11 /product="putative major head protein" /protein_id="ASN72768.1" /translation="MAQGTTTLSTQIVPEVLAPMMQAELDKKLRFASFADIDNTLVGQ PGDTLTFPAFVYSGDATVVPEGQKIPVDKIETNRREAKIHKIGKGTDITDEALLSGYG DPQGEAVRQHGLAIANKVDDDVLEALKGTKLNVSSDIGTLAGLEAAIDTFDDEDLEPM VLFINPKDAGKLRASASENFTRATELGDDIIVKGAFGEALGAIIVRSKKLAEGEAILA KRGAVKLITKRDFFLETDRDPSTKTTALYSDKHYVAYLYDESKAVKVTKGAGA" gene complement(8908..9507) /locus_tag="10S4_13" CDS complement(8908..9507) /locus_tag="10S4_13" /note="putative similar to phage phig1e RORF204 (minor capsidprotein); Similar to NP_510937.1 [Staphylococcus phage phiETA] (BLASTp score : 326). Similar to PF06810.6 [Phage_GP20] (hmmscan score : 152.6)." /codon_start=1 /transl_table=11 /product="minor capsid protein" /protein_id="ASN72769.1" /translation="MDLYALLGRFKDGEIEKQKVLDAIDESKSGMVPRSRLNDKNAEI EELKTEISNRDEQIAKLQDSVKDDSEIQKELDELKNKNAEWQSKYQESQLNNAVKLAV AKEANDANDILAFINQDELELQEDGTVKGLDKAIESLKESKPYLFADNVPKGNTPQDG NNPEFGLTKEQFDNMSVAERTELFLNDRETHDKLLNQNQ" gene complement(9609..10580) /locus_tag="10S4_14" CDS complement(9609..10580) /locus_tag="10S4_14" /note="Similar to YP_008059859.1 [Staphylococcus phage StauST398-3] (BLASTp score : 508). Similar to PF04233.9 [Phage_Mu_F] (hmmscan score : 103.6)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72770.1" /translation="MASKNNQNKSNPKINNQNDIDNYIDYLIRQSEKEIEVLFANRLK VIKQEIADMYDKYQSDDPHVTWTEFNKYNRLNKELTRIGEMLTEDYREVAKAIQKSQK DAYIEKFMMSLFLYEMASQTSMQFDVPTASVINKAIEQPIEFIKLIPTLQKHRDEVLK KIRIHITQGIMSGEGYSKIAKALRDDIGMTKAQSLRVARTEAGRAMSQAGLDSAMVAK DNGLDMKKRWSATKDTRTRDTHRHLDGTSVDIEDNFKSSGCVGQAPKLFVGVNSAKEN INCRCKLLYYIDEDDLPTTMRARKDDGTTEVIPHMTYREWEKYKRNG" gene complement(10525..11943) /locus_tag="10S4_15" CDS complement(10525..11943) /locus_tag="10S4_15" /note="putative ORF006; Similar to YP_240382.1 [Staphylococcus phage 71] (BLASTp score : 710). Similar to PF05133.9 [Phage_prot_Gp6] (hmmscan score : 375.3)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72771.1" /translation="MPWDKPYGEEIVEQIQPKAETQEEMIVRLIRNHEQNVERISVGQ RYYDKNNDIYNQRPKFDLDGNVDLNKPDWRINTNYHQNLVDQKVAYLATNPVSYSCEN EKILDTIHDVLDNRWDNKLIDVLTAASNKGVDWIQPYINEQGEFKLFRVPAEQAIPIW TDKERETLQAFIRIFRLNDETKVEYWTADDVTYYVYENGQLINDYYYGENNKQTHFST GSWERVPFIPFKNNPEEMSDIWMYKSIIDAIDRRLSDTQNMFDESAELIYVLRGYEGE DLKEFMQGLKYYKAINVDAEGGVETIQVEVPVASTKEFLDIMRSNLIEFGQGVDFQTD KFGSAPSGVALKFLYTGLDLKANKLKNKATVAIQELLQFIIDFYKLNVDVKDIEITFN FNRTMNDLEQSQIGAQSQYLSKETIVRHHPWVDDPQAELERIEQEQIEYNQQLPDIDD GGAVNGEQEQPEQEQSEDKQPK" gene complement(11962..13227) /locus_tag="10S4_16" CDS complement(11962..13227) /locus_tag="10S4_16" /note="Similar to YP_950600.1 [Staphylococcus phage CNPH82] (BLASTp score : 778). Similar to PF04466.8 [Terminase_3] (hmmscan score : 361.4)." /codon_start=1 /transl_table=11 /product="putative terminase large subunit" /protein_id="ASN72772.1" /translation="MTISLNLSELIPKHFHPLWKATKDPNILNVVAKGGRGSGKSSDI SIIITQLIMRYPMNAVVVRKTDNTLATSVFEQIKWAIEEQKVSHLFKIKVSPMEITYI PRGNRIIFRGAQNPERLKSLKDSRFPFSIMWIEELAEFKTEDEVTTITNSMLRGELDD GLFYKFFFSYNPAKRKQHWVNKKYESSFQPDNTFVHHSTYLNNPFISKQFIQEAESAK QINELRYRHEYLGEAIGSGVVPFNNLQIEKIPDELYNTFDNIRNAVDFGYATDPLAFV RWHYDKKKRIIYAVDEHYGVQISNREFANWLHKKGYQSDEIFADSAEPKSIAELKQEH GVRKIKGVKKGPDSVEHGEQWLDDLNAIVIDPSRTPNIAREFENIDYETDKDGNVKPR LEDKDNHTIDATRYALERDMRQNHLNILR" gene complement(13211..>13657) /locus_tag="10S4_17" CDS complement(13211..>13657) /locus_tag="10S4_17" /note="Similar to YP_950599.1 [Staphylococcus phage CNPH82] (BLASTp score : 223). Similar to PF03592.11 [Terminase_2] (hmmscan score : 126.0); alternative start codon" /codon_start=1 /transl_table=11 /product="putative terminase small subunit" /protein_id="ASN72773.1" /translation="DVISDMKLTIRQQKLVDEYIKTGNAYQAAINAGYSESYAKVDVH KALAKPSLKQALDERMAELKKKSIADQDEILQYLTSVVRGEITDQELIPIGVGKGQME VEELEKRSDTNARTKAAELLGKRYMMWTEKHQVETNATVQFNDDIT" ORIGIN 1 gactagcgcc atggctagat gtgtataacg tatcaggttt agtcatacga ccatcaataa 61 caggtggtgc gtgtctttta ccgactaaat ctgctaaagg gtgtgtgtat tcatactgca 121 gacttgcttc attatatgtt tgttggtcgt caaaaccacc aaaaccttta atataggtat 181 aacattttga ggcgtcctct tgaattttta cattgttagc gttgacacct gctttaatat 241 aataatccgc tttatgttca acactatcaa acaaactaaa tgtttttgta tttgcatcat 301 attcatattc taggtcgtat ctttccaacc cttttttgaa caattcaagg ttagtttcat 361 gattacctag attctcaaat ttagatgatt caactttttt attcagaata tacttataac 421 ctgtactttt gaatactaaa tcaaaatact ctttaccggt aaaactaccg ttgtatgttt 481 cataaatgcg agtattgttt aaatcatcta gttcgactgg acgagcttta acacttattt 541 tcattttctc gcctactgtc gttttgtcta acatgacaat tctatattct ctagggtctc 601 cgtcaccgcc aatattacta accgtccaca ttttagtaat tgaaccgatc gcgtcaaaag 661 tagcattgtt ttcaactatt tcaaattcta atgaaccatc ttcatttaac ttttcattca 721 atttcgtttc tacctgtaaa gggtactcac gaccttgtaa tgtttttaaa gctatagcca 781 ttcagcgacc tccttacatg tagtaacgtt tgtgtttaaa cacaactttt tgtagtgttt 841 tggtgctgtt aaaactgttc caaccaggat acaaaacagg ttgttgtttt gttgtgttgt 901 attcatcaat tctgatgtta tttctatatg tgtgcaaacc atcaaatttg atgacatcac 961 ctgcacgcat ttctaaacct gctattttca ttctatcgct atattgcaag tagaatgtga 1021 tacctgcttt atcatcttta cttatattct caccaagcgt taattcaaca acgctatctt 1081 ggttaaattg tttgatttca acagtaccac cataatatac aacacctgtt ttacaatcat 1141 agaaagttga acgtctttct tggtcaggta catcaaacgg gttgtcatca ggaattcccc 1201 atttttccaa gttaccatct tctttttcta aaatagtgtt ataagcaatg ctttcaaaat 1261 aaggtaactc taatgtttca aattcaagtg tgaattctcc ggatgtttga gttgtatcaa 1321 atgaaatatc attaactaat ccaactttga tttgtctgcc gtcaacatac tctagctcaa 1381 attcttgttt ttgaggtgca aacggacttt caaactttat ctttgcctct ggtactgcaa 1441 gttctcttaa atataattcg cctctcaata aagcctgtaa tcgagctttt aagtgagagg 1501 cataagcaat tttttcaaca ctataaaaca atttgattgt aacttttttc ttttcttcgg 1561 tagtagagtt gtgaaatcga ccattaacac gatcaatgtt atcaaacttt cgctcataac 1621 ctgcaccttg tacatcgtaa gaaataaccc tcaacgcttg atgtgtatag cgattgtcac 1681 taataaagtt actttctttg tctttaatta tttctatatc atgtaatatc aatcgacaat 1741 cactccttta gtataagtcg aaacttgcgt cttttgagtt actatcttca atgtatgact 1801 taatagcagg tatgtctgat tcgttacgta cagttacatt aacaacaggt ttgttattct 1861 cttgcatact gtgtcgaaca tcacggctca tatgagcatt tacatcacta tttaaaccac 1921 cacttaaacc agtggctaaa tcactgctta attctggttg gaatgcgtcc ataactttac 1981 gcgacgcttg tttaacgctt gatactgcag tacctacgtg tttatcaatg cctatgccaa 2041 aaccttgcat agtaaacata ccaacgctca tgaattcacg agatggagag tgaataccta 2101 atacagattt agcagcatga agtgcgctag acgctgcgtt tttagctgca cttacaaccc 2161 aagccatacc ttgtttgata ccgtttacta aacctctcat catatcaagt ccggcactaa 2221 tgaaactttg tgcaaaactt ttaactttat taacagcatt acgcatacca tttccgactg 2281 agttaactac ttggaaaaaa ccactaacta ctctgtgaac aaaattagtc atcgtattcc 2341 aaatagatga aacccattgt gcgccatgag atttgacgta attccaagct tgaaccattt 2401 tcgagccgac agttgaaact acgcgaccaa accagttaga tacagaattc caaattctag 2461 taacaaaatc tgtgattgcc gaccaaattt gagaccaact tgtaatgtta gtaccaagta 2521 ttcggtttag cacactgaaa ataaaattag aaatctgaga ccaaatggat actagcgtat 2581 tccaaatcgt gttcattacg tttgatacca tgttttgcaa tgtcaaccaa gcgccagaga 2641 aatctccagt taataattgg attaatgcag tgaataatcc aacaataatt tgaacaacaa 2701 ctgaaataat accgccaatc gcagtaaaga tgactgatac cgctgtccat aaagcttgga 2761 aagttaatat caatgcttga atgataccca tgactaatac acctagaacc tgcatgaaca 2821 cctgtcctag catttgaaga attggcataa ttggttgtaa tgtttgttgg attgacgccc 2881 acaattgcat gaaccagtta ataatagaac tgattgcacc agaaatcgca gtaacgattc 2941 cattccacgt ctctgtaatc atttgtcgga atccctcgtt ggttttccat agatatgtga 3001 ttattcctac taatgcaaga ataacgccaa taacaatcca cacaggagcg gatataccgg 3061 caattgcagt accaaccgca gcaaaagctt cgcctaacat tggcagtaaa cttgctaaga 3121 atttaaacgg actagctagc atttcgaata ttgtcgaaag caattgtcca gatttagcaa 3181 gttgagcaat tttcgtaata acttgaacaa cactcatacc aaacacatta cttaaaactg 3241 agctaatagc tataactggt gccattaaag cccagaatat accgcctaaa atcatcatca 3301 ctccgaccat tctagctata gcagggtgtg tttcgaataa cttagcgata aaacctgcta 3361 atgcagtaac aaatttgagt attactgagg cgataggagc cattgcagta ccaaatgcga 3421 ttaacgctct aacgatgtta cctatcaact gaccaataac cggaccattt tgttgtacat 3481 actgaacaaa cttcttaaag ccctcagatt taccaacttg ttcagaccat gctctgaatt 3541 gacctgttaa tttaaccaac caatcaaaga tacctgcgct attttgagcg aatgcagcca 3601 ttaaattacc aataccagca aacacattgc caaatatctg acctatttta ggtaggttag 3661 ttttagtgta ttcaatgaat gatctaatag cgttttgacc tgctgcacta ttagcccatt 3721 tctggaatga cgcacctagt ttatttaact gttgagccgt ccacaagaac aatggcgcta 3781 actgtgtaaa tacgttaact aatccgtcgc cgaatcgtcc tgcagcactt aatagggtat 3841 tgaacgtttt aacacctgtc gtattcatca tattgaagaa tttcgatgca gtttgactgt 3901 gttgtgccca tttaagcatt cctgccgacg cttgttccat tccttttgct acaccagata 3961 agaaaggtgt catacgttgt aaagctacat taactgtttg taatccatta gctaatgtat 4021 tgaatatttg tgcttgattt tgcttaataa tcccttgcca tgtagattgc acttgagata 4081 atgcagattg ataacgtcta gtttctgcag tagcttgcaa tgttccgtca ttcaacatct 4141 taattgcact agtagccatt atgccgaatg ccattgcgcc tgctcctgct ataccaaatg 4201 ctcctgccaa accaattaca ccgccaccta atacaccaac tgcattaagt accgccataa 4261 ttgcaggaac taagctagct ataacaggta ttaacgcttg aatactagca atcattaaac 4321 cttttacttg ttggctaaag atagtaccaa atgttctaat gttagacgct atgctatcca 4381 ttttagtttg atattcatct aatgatcgct tagcgatagc cattgcactc tgtaactttg 4441 tcataccagt agtatcaaca tctagcttaa ctgtgtgttt acgccatatt gctaatctag 4501 ctttagctgc agtgactact ctatttaaag gagaggcgtc cccgtcaata tctactttct 4561 tatctctaat tgcatttaac ttacctttta ttgtggcaat cactcgttga ataggggttg 4621 cattagcgtc tatttcaacc ttgtgttctc gccaacgttg agccattgct ttagctctgt 4681 ttaatgctcg ttgaaatcta cttatgttag cagtaacttc tgtttcaatt tcatctggaa 4741 tactcgtttt agccatttgt tgagctttgc gaatgttcct ttgaaaattg ttaatgatcg 4801 cagacacacg agcaacaaag tttttgttca ttgctaccct cctttatgac ttgtgttacg 4861 cattttgtcc aagaaattac gtgtaccacg tttttgaatt tctctattgt gtttattttg 4921 tgctagctta cgttttctca tacgttcgta ctcttcttct tcaccacgca ctacataacg 4981 tgcatgatca atttgttttt gtagacgttt gagagattta cctgcttgga ctagtccgtt 5041 agcttgcgct acaaacaaca tattttcttg ttggtcgagt agcgcctgtc tacgtcctat 5101 aatccagtct ctccactcat taggcgttaa gctcatgagt tcgtcatagg ataggtagcc 5161 tatgtattga ctggttaact gtcttatttc tgaataatct agtaaggtag ctcgcccatg 5221 atttctttgt agttgttctt catgaattcg attccgttct tcgtagattc tttctcgtcc 5281 tctttcacca tagatggtgc tgagttcatt tgtgtccaga acattcgaga tttttgtttg 5341 aaaaaaccac tattatttaa cttatctaaa gcaccttgta gtaattcaat agtatcttgt 5401 ttttcttcaa taacttctga taaagccatt tcaatgtctt cacgtttagg tgcgttctta 5461 cctaaataag cagtagcaca ttcccaaaag tctgcgatag catttgtatc acgttctaaa 5521 agtccattat agatagcatt aaagccggat actttagttg tctttccgtt ttcatcttgt 5581 tcgtctctag caaattgttt agctttgtgg tcgaaggcaa atactgtttt agcttcgatt 5641 tcttctccat taatatttaa tgtagtgata ggattgattg tattttcagt cattttttaa 5701 cctctttctg atattttgta caaaaaaata gagggcgtta tgccctcatg taatctatgc 5761 tccagatttt ggtgtacggt tttcgtaagt atctgtatat gcgcccatat cttcccactc 5821 aacagtagga gcttgtgcac tagggttaag ccactctgga ggtaattccg aaaccttacc 5881 atcagcacta ttgaatttaa cttttgcagt aatttcaatt ttgtcatctt catcatcaaa 5941 tgaccattcg tgttcttcta caattacata tgcaaaagta ccgtgatgga aaccttcacg 6001 tttcttcact tcccaaatcc aaatacgtaa ctgtttgaag tttttaactg cttcttttaa 6061 tgcctcttgg cctttgtcac ctggaacacg atcaaccgtt agtttaattt cttcttcaac 6121 agaattacga ccataatctt ttttaccgcc ttgaattatt tcagcaaggt cgttactgat 6181 tgtatgaccg ccctcagcta aactagctaa taaaatcgcg tcttcttctt tcaaagtaga 6241 ggcaagctct ttatctgcaa tctgaattgc tgcgatatat ttattctgag ccattcgtta 6301 cactcctttt tcttatattg tgtctgtatt taaaaacaag ccgaatgata ccgtgtttgg 6361 tgtactgatc tatgtcagta atcacttcct gtgtatcaat tcgactttta ataaatgaat 6421 agttatttat ttctatttca gaatttagga cataacctaa aaactgtatg atttgcgacg 6481 cctcgttgcg attgtaagct tggctataca catggaatgt aatgccaaca tcttcaacca 6541 tgcttgtcgt cgtttcatca ttagtgacgt ttgtttcacc cacaacgata tatgggtaaa 6601 cagcgtcttt ctgaacgcaa tcaaaaaccc taccaccaag ttgtttattg atgatagggt 6661 tgctttttaa tttgttatat atctcattga atagatatcg ttcaactgat acccacatat 6721 cttaaccacc ttatgaaaaa tacttattga atacttgcgt gcctgcgtca atagcagggt 6781 tccagaacgg ttgagcagga ctaccgtaag tagtgtgcca atccccgtct gcgtctttat 6841 aagaccacgg taattttgta gcacggctac cgcctggtcc ctctgcatat atacctgtac 6901 catattcaac atatatagca tattctgcac caacacttat aatgcctgtg aaaccaccat 6961 ctttgtacat gaagtcaata ctttctttta agtaacccat gtcaacaggt gccatttcaa 7021 ccgcagtatt ataaatttta agtgtagtct tagctatacc ttttttaacc caccgttcca 7081 tttctttctc gtaatcttcc aactctttaa ctaaatccca attaccatat ttaaccttag 7141 ccaataggac attctcgcaa tctagttaag ttaatttcgt tttgtccacc ttgatcaaca 7201 ggttctccta caacttcata cgttttaccc tgatatttaa ataatgtttt agtagttatt 7261 ggtatgttgt atggcgtata taggtttctg tcatagtctt ggctcatttg atgatatttg 7321 agttgttcag atgaagtagg cgtatccata aaacctttga tagtgttttc gcttttaaag 7381 cgctcttttt cattaggata cttacctata acttcacgaa caccaattga aattacatgt 7441 ggaaattcat cgtatggatt aaacatgata accactccaa cgtaagcgtc tgaatggttt 7501 taaatagcta tacgtttcat ctggtaaatc agtcacaaaa gaatagctaa ccgttcccat 7561 agtacgagag gaaacattgc cagttgtacc gtgctgaata caattagcta taaatttctc 7621 tacgctacta ggtatattct ctttgtcgaa agtttggtta caatattctt ctgctacttt 7681 cagatactta tcaataagca tttctaaagt atcatcttgc gaagtgtcat cgagtgaaag 7741 attatttaat agtttgacgt cttttgcgtc cattactcaa cacttcccaa cacttcgaca 7801 agctcatctt tcttcatctt cgtatagccc tcaatatcac gttgtttagc gagttcttta 7861 agatctgcta ctttcatatc agataactta gtttgctctt ctacacgctc tatgagtggc 7921 ttacgttgac ggttattttt tgtggataat tcagttaatc tttcttcgct tacattatcg 7981 tgattaaacg tatcacctac attatattca cgatcattat cttgtaagtc tacgaaatac 8041 tcgattactt tatacatacg tcactacctc ctgttatgcg cctgcacctt tagtgacttt 8101 aactgcttta gattcatcat aaaggtaagc tacataatgt ttgtcactat ataatgcagt 8161 agttttagta gatgggtcac ggtctgtttc taggaagaag tcacgtttag tgattaattt 8221 aaccgcacca cgtttagcca aaatagcttc accctcagct aatttttttg aacgtacaat 8281 gatagcacct aatgcttcac caaacgcacc tttaacgatg atatcatcac ctaattcagt 8341 tgcacgtgtg aagttttctg atgcgctagc acgtaattta ccagcgtctt taggattaat 8401 gaacaatacc attggttcta aatcttcgtc atcgaatgta tcaatcgccg cttctaaacc 8461 agctaatgtg ccgatatcag aacttacatt taacttagta ccttttaatg cttctaatac 8521 gtcatcatct actttgttag cgatagctaa accgtgttgt cttactgctt cgccttgtgg 8581 gtcaccataa ccagataata aagcttcatc agtgatatca gtacctttac caattttatg 8641 aattttagct tcacgtctgt tagtttcgat tttgtccact gggatttttt gaccctcagg 8701 aactactgtt gcatctccac tgtatacgaa tgctggaaat gttaatgtgt ctcctggttg 8761 acctactaat gtgttatcaa tgtctgcaaa cgatgcgaaa cgtaactttt tatctaattc 8821 cgcttgcatc attggcgcta atacttctgg tacaatttgc gtgcttaaag ttgttgtgcc 8881 ttgtgccatg tttttaacct ctttctatta ttgattttga tttaataact tatcgtgagt 8941 ttcacgatcg tttaagaaca attcggtacg ttctgcaacg ctcatgttgt cgaattgctc 9001 ttttgttaat ccaaattctg gattgttgcc atcttgtggt gtgttacctt tcggcacgtt 9061 gtcggcaaat aaataaggct tagactcttt aagcgattca atcgctttgt ctaaaccttt 9121 gacagtgcca tcttcttgta attccagttc atcttgattg atgaacgcta gaatgtcatt 9181 agcatcgttt gcttctttag caactgctaa cttaacagcg ttattcaatt gtgattcttg 9241 gtatttactt tgccactctg catttttatt tttaagttcg tcgagttctt tttgaatctc 9301 gctatcatct tttacagagt cttgtaattt ggcaatttgt tcatcacggt tagaaatttc 9361 agtttttaac tcctcaatct cggcgttctt atcatttaaa cgagaacgtg gtaccatgcc 9421 cgattttgat tcatcaatag cgtctaatac cttttgcttt tcgatttcac cgtctttaaa 9481 tcgccctaat aatgcatata aatccatgtg taaactactc ctttttacga gttttacgtg 9541 caacgccacg aagaattttg tataaaaaga agcagtttaa cgacatgcta aggtcgagta 9601 gtaggttact agccattacg tttatatttc tcccactcgc gataagtcat gtgtggtatt 9661 acttcagttg taccgtcatc tttacgcgct ctcatagtag taggtaaatc atcttcatct 9721 atgtagtaaa gcaacttaca acgacagtta atattctctt ttgcactatt aacgccaaca 9781 aacaacttag gtgcttgtcc cacacaacca ctagatttaa agttatcttc aatatctact 9841 gaagtaccat ctaaatgtct gtgtgtatca cgtgtgcgtg tgtctttagt agctgaccaa 9901 cgtttcttca tatctaaacc attgtcttta gctaccattg cactatcaag ccctgcttgt 9961 gacattgctc tacctgcctc agtacgagct acacgtaacg actgggcttt agtcatacct 10021 atatcatcgc gtagagcttt agctatttta ctgtaaccct cgccactcat aatgccttgt 10081 gtgatgtgta tgcgtatttt ctttaacact tcatcacgat gtttctgcaa cgtaggtatt 10141 aacttaataa actcgatagg ttgttcaata gctttgttaa ttacagacgc agtgggtacg 10201 tcaaactgca tagatgtttg acttgccatt tcatataaaa ataggctcat catgaacttt 10261 tcgatataag cgtctttctg tgacttctga atagcttttg ctacttctct atagtcctca 10321 gttaacatct cgcctatacg tgtaagttcc ttgttgagcc tgttgtattt attaaattca 10381 gtccacgtaa catgtgggtc atctgattgg tatttatcgt acatatctgc tatttcttgt 10441 ttaattacct ttagtcgatt agcaaatagc acttcaattt ctttttcaga ttgcctgatt 10501 aggtaatcaa tatagttatc gatatcattt tggttgttta tcttcggatt gctcttgttc 10561 tggttgttct tgctcgccat tcacagcacc tccgtcgtcg atatctggta actgttgatt 10621 atactcaatc tgttcttgct caatacgttc aagttctgct tgcggatcat caacccatgg 10681 atgatgtctg actatagttt ctttagataa gtattgagat tgagcaccaa tttgtgattg 10741 ttctagatca ttcattgttc tattaaaatt gaatgtaatc tcgatatctt ttacatctac 10801 atttaactta taaaaatcga taatgaattg tagtaactct tgtatcgcta cagtagcttt 10861 attcttcaac ttgttagcct ttaaatctaa tccagtgtat aaaaatttaa gtgctacgcc 10921 actcggtgca gaaccaaatt tatctgtttg gaaatcaaca ccttgaccaa attcaattaa 10981 gttagaacgc atgatatcca aaaactcttt tgtgctagca actggcactt caacttgtat 11041 tgtttcaacg ccaccctctg cgtcaacatt gatagcttta tagtatttaa gtccttgcat 11101 gaattctttt aagtcttcgc cctcataacc acgtaaaacg tagattaatt cagcagattc 11161 atcaaacata ttttgcgtat cagataagcg tctatcgata gcgtcgataa ttgatttata 11221 catccatata tccgacattt cttccggatt gttcttaaat ggaatgaatg gtactcgttc 11281 ccaactgcct gttgagaagt gagtttgttt attgttctca ccgtaataat agtcattgat 11341 cagttgtcca ttttcataca catagtatgt aacatcatct gcagtccaat actcgacttt 11401 agtttcatca tttaatctga atatacgtat aaacgcttgt agagtctcac gctctttatc 11461 agtccaaata ggtattgctt gttcggcagg tacacggaat aacttaaatt cgccttgttc 11521 attaatataa ggttgtatcc agtccacacc tttattactt gcagcagtta atacatcaat 11581 caacttgtta tcccaacggt tatctaacac atcgtgtatc gtatctaata tcttctcatt 11641 ctcacatgaa taactaacag gattagtagc aagatatgca actttctggt ctactagatt 11701 ttgatgatag tttgtattaa tccgccaatc tggtttattc aaatcaacat taccgtctaa 11761 atcaaattta ggacgttgat tgtatatgtc attattctta tcgtaatagc gttgtcctac 11821 actaatacgt tctacattct gttcatggtt acgaattaat ctcacaatca tttcttcttg 11881 tgtctctgct ttaggttgta tctgttcgac gatttcctcg ccatatggtt tatcccacgg 11941 cattctaata atgttaatca cctaccttaa aatatttaaa tgattttggc gcatatcacg 12001 ttctagcgca tatctagtag cgtctattgt gtgattgtct ttatcttcta acctcggttt 12061 aacgtttccg tctttatccg tttcatagtc gatgttctcg aattctctag ctatgttagg 12121 tgtacgacta ggatcaatca caatagcatt taggtcatct aaccattgtt cgccatgttc 12181 aacgctgtct ggacctttct taacaccttt aatctttcta acaccatgct cttgtttaag 12241 ctctgcaatt gatttaggct ctgcgctatc cgcgaatatc tcgtctgatt gataaccttt 12301 cttatgtaac caattagcaa actcacggtt acttatctgt acaccgtaat gttcatcaac 12361 tgcatatatg attcgtttct ttttatcata gtgccaacga acaaatgcta acggatcagt 12421 agcataacca aagtcaactg cgttgcgtat attgtcaaat gtgttgtata actcatctgg 12481 tatcttctca atttgtaagt tgttgaatgg tacaacacca ctaccaatag cctcacctaa 12541 atactcatgt ctatatctca gttcatttat ctgtttggca ctttctgcct cttgtataaa 12601 ttgtttggat atgaacgggt tatttaagta agttgagtga tgtacaaatg tgttatctgg 12661 ttggaatgaa gattcgtatt tcttattaac ccagtgttgt ttacgtttgg cagggttgta 12721 tgagaaaaag aatttgtaaa acaatccatc atctaactcg ccacgtaaca ttgagttagt 12781 tatggttgtt acttcatctt ctgttttaaa ctctgctaac tcctctatcc acatgataga 12841 aaaagggaat cgactatctt ttaacgattt taatcgttct gggttctgcg cccctctaaa 12901 gataattcga ttccctcttg gtatatatgt tatttccatt ggtgacactt taatcttgaa 12961 taagtgtgac accttttgtt cttcaatcgc ccatttgatt tgttcaaaca ctgatgtggc 13021 taacgtgtta tctgtcttac gcactacaac tgcattcata ggataacgca taatcaattg 13081 tgtaatgata atggatatat ctgacgactt accactacca cgtccacctt ttgctactac 13141 gttgagaata ttagggtctt tggttgcttt ccacaatggg tggaagtgtt tgggtattaa 13201 ttcagataag ttaagtgata tcgtcattga attgcaccgt cgcattagtt tcgacttgat 13261 gtttttctgt ccacatcata tagcgtttac ccaataactc tgcagctttc gttcttgcgt 13321 tagtgtctga acgtttttct aattcttcca cttccatttg acctttacca acaccgatag 13381 ggattaactc ttggtcggtt atttctccac gtacaacaga agtaagatat tgtaatatct 13441 cgtcttggtc tgcaatagac tttttcttca attccgccat tcgttcgtct agcgcttgtt 13501 ttaagctagg ttttgctagg gctttatgta catctacttt tgcgtaactt tcgctataac 13561 cggcatttat tgctgcttga taagcgttgc ctgtcttgat gtattcatca actagctttt 13621 gttgtcttat tgttaatttc atgtcactga tcacgtc //