LOCUS MF417975 13110 bp DNA linear ENV 27-FEB-2018 DEFINITION Uncultured Caudovirales phage clone 7S_6, partial genome. ACCESSION MF417975 VERSION MF417975.1 KEYWORDS ENV. SOURCE uncultured Caudovirales phage ORGANISM uncultured Caudovirales phage Viruses; dsDNA viruses, no RNA stage; Caudovirales; environmental samples. REFERENCE 1 (bases 1 to 13110) AUTHORS van Zyl,L.J., Abrahams,Y., Stander,E.A., Kirby,B.M., Clavaud,C., Farcet,C., Breton,L. and Trindade,M.I. TITLE Novel phages from South African skin metaviromes JOURNAL Unpublished REFERENCE 2 (bases 1 to 13110) AUTHORS van Zyl,L.J., Abrahams,Y., Stander,E.A., Kirby,B.M., Clavaud,C., Farcet,C., Breton,L. and Trindade,M.I. TITLE Direct Submission JOURNAL Submitted (29-JUN-2017) Biotechnology, University of the Western Cape, IMBM, C2, Life Science Building, Robert Sobukwe Rd, Cape Town, Western Cape 7550, South Africa FEATURES Location/Qualifiers source 1..13110 /organism="uncultured Caudovirales phage" /mol_type="genomic DNA" /isolation_source="healthy skin from scalp of human male" /db_xref="taxon:2100421" /clone="7S_6" /environmental_sample gene complement(117..425) /locus_tag="7S6_1" CDS complement(117..425) /locus_tag="7S6_1" /note="Similar to YP_005087069.1 [Rhodococcus phage REQ2] (BLASTp score : 71.6)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72800.1" /translation="MKWPWSKDVDDARRQAQEAAEQHAQAEQQRLEVKVQSEDAKKVA RVLRRALERHGYTAVSYTHLRDNETKVKLVCRRLLAKQNTKQATPVNHAHSIEVDIDT " gene complement(422..715) /locus_tag="7S6_2" CDS complement(422..715) /locus_tag="7S6_2" /note="Similar to YP_005087068.1 [Rhodococcus phage REQ2] (BLASTp score : 162)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72801.1" /translation="MQILAPSAWEGASVVTVVIVLALLHGLALVRGWIVWGPAHREIM RLKDDAINHSQGREQADQDTIRTLADTIAEQKVASQLAAHASQSIHEVVGRAP" gene complement(715..1002) /locus_tag="7S6_3" CDS complement(715..1002) /locus_tag="7S6_3" /note="Similar to YP_005087067.1 [Rhodococcus phage REQ2] (BLASTp score : 122)." /codon_start=1 /transl_table=11 /product="putative holin" /protein_id="ASN72802.1" /translation="MNPNPILDVLRAKMAEQPLVKKYANTVATAVAAVVAVLWTVISV GVNLPDQVVVGGLLLASLGTVVGVKFTPNGVTDKQIDELEAYTRRNVPRGE" gene complement(1002..1625) /locus_tag="7S6_4" CDS complement(1002..1625) /locus_tag="7S6_4" /note="putative Pas66; Similar to YP_024852.1 [Actinoplanes phage phiAsp2] (BLASTp score : 68.6)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72803.1" /translation="MAWRTAIALDEYRAAINARFPNRSKASDGTIGDVAHAGQGQGSD HNPWVVRNGIGVVRAFDLTHDPRNGVDCEKLSDYLFDMGKAGDPRLRNGGYLIFNNWI TNADWGAWRRYNGSNPHTSHMHVSFSADFFDNRGDWSRALGPAAPKPPVGGSMNIEQE IRNQLTGSAELGKYPGWKQLGNRTVVDALAVIGAKLNIEGFDPKAGK" gene complement(1627..1917) /locus_tag="7S6_5" CDS complement(1627..1917) /locus_tag="7S6_5" /note="Predicted by MGA" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72804.1" /translation="MATAEMVVDGLPGFAGLATLYRIEPPIQGTDHLIVYHRPRVAGQ PGQMTVTLGTEEGVSLSADIRPQPGTYITDEPNHHLALQLAGGYRIVENGGM" gene complement(1927..3099) /locus_tag="7S6_6" CDS complement(1927..3099) /locus_tag="7S6_6" /note="Predicted by MGA" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72805.1" /translation="MTVPWGDYPDGTFDDDLSGLNGIDEASWRASKFGSIGGFNQAHE RFKAEFISGPLFNGEVVRLDNRIDEIVVGTERAILWTFSEYGVWTKHPAAYKVVVDVL SGASGGYQGTNGSGTSGAGGAGGFSGGWERFVFTGAALEALPSSVAVTIGGGTSGGST ATAGTSSFGSFGSATGATPSNYGSGSRTYKMRGGSGGSGSVIAGSDGSAGPFSSGGSG GSPGSINGGNGFSAGVGQIGTGSAGGGGVANTSLFGTGGKGGHGGWPAAPGGGGGYGG ANGGGVGNGAGGAVFVTVYVEDTLGVPPSTPTGLAASAITRTSARVTWAASIDDVMVK NYILYLNGTRYAVVESTYHDFVGLTASTAYAVRVQAVDIGDNVSGLSTTVNFTTTA" gene complement(3096..3506) /locus_tag="7S6_7" CDS complement(3096..3506) /locus_tag="7S6_7" /note="putative gp22; Similar to YP_009018710.1 [Mycobacterium phage DS6A] (BLASTp score : 76.3). Similar to PF10910.3 [DUF2744] (hmmscan score : 69.0)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72806.1" /translation="MRDKELPTIDKCDLSDPEEVFLWTYLGLPDVIGADLVWPIPFWR KVSAHQIDCGLMLQCPSCGHREQPKKKYRIDATEDVMMGGGGRWVPADAPEPERDVLA ERLDAIRPEVKREMLRRLREQFPDDAGLQDGGGS" gene complement(3506..5194) /locus_tag="7S6_8" CDS complement(3506..5194) /locus_tag="7S6_8" /note="putative gp19; Similar to NP_817992.1 [Mycobacterium phage Che9d] (BLASTp score : 286). Similar to PB003228 [Pfam-B_3228] (hmmscan score : 297.3)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72807.1" /translation="MATALEQACADGLAAVRAREASLEAFRLIAPVIKLWDGNIGLQH VVRCENEYDWLDVENDTGPGIVKIDADFEEGQYLWDIKGRKDRGETIDVLVTVDYVGR RWSGFLDEVTMETNDLGQSIVTANFLSDYEQLKWRHLWATPSTPAGFQPFKVFMLGGP TDWALGTALKLNLARAHGRPSGMGWMDPLADGAADYREWPIVLAPLDYETAASRGTVT GLALSRFKTYHEASEAMVEDADITTRWRRYMPGDALPWPGAQISYGTLVIWFENNSGG LAPTTTGTFLGGIGQLVRIFVSSLVEGIGGGSLPIDNGEQAIVGIPAVPEYQVPGYLG TEPKGPYVFYPAGSPGVLSQSAKIKIGRGHRLTGGGHSMPGVNEVISAAVQALGDALA AIPLAPIPPLGGVADALLKPFYEDTILAFMTVYLVNRSAHLSHFSLYEMWVEGADQAY TLSATLVLREAVRATETKYTADMEIIDGSPWYVGAKGYGHFDLGNRILVQTEGDRSGR IEAPRVKSLRLTASPGVDPAFAIKLGSKDPVDAFGQVLKRIQKLASGLKAMGMI" gene complement(5195..6109) /locus_tag="7S6_9" CDS complement(5195..6109) /locus_tag="7S6_9" /note="putative gp20; Similar to YP_009018708.1 [Mycobacterium phage DS6A] (BLASTp score : 180)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72808.1" /translation="MAFTISVLGAGGGPETVVSGPGDIASPFVLGEDQVKGLYHAPVE SEWKRARRQRGGSLAGVENPTRDMQLGFHVRGDDRMSFTEADDLLRSCFTYHLDPWWP GDTLARIAVESGDDYRTLSVQMHQEPEFAPRMDPDQLRYGNVFYRLRAAQPFWESKTD VTHWETSGTSGSGTITVSNPTDVEMFQAWVLTRGTWTIPDVSWTGRPRKRTPGGLFGS RSIPLKPVTSTHGTLTINLDPMHVMMQSSNGTNVMGEVGGGYYFMHTIPPHTPKTQLP ISVTGAPAGGARAELHQPRLWSNFYGGR" gene complement(6110..10981) /locus_tag="7S6_10" CDS complement(6110..10981) /locus_tag="7S6_10" /note="Similar to YP_009043620.1 [Mycobacterium phage OkiRoe] (BLASTp score : 212)." /codon_start=1 /transl_table=11 /product="putative tapemeasure protein" /protein_id="ASN72809.1" /translation="MAGGVELGVGYLSIVPETSKVVPGINSALTSAQGGADKAGRGIG GKLASGIGSTLKFGAATAGAAAAGVIGTALTQGMKRVVAIDDAKGKLAGLGHTAEGVT TIMDSALASVKGTAFGLGDAAGIAASAVAAGIKPGQDLTKYLKLTGDAATIAGASLGE MGSIFNKVQTSGKAYTDNLNQLADRGIPIFQWLQDEYKVSADGLSKMVKEGKVDAETF NKVIEKNIGGAALESGKTLRGSFENMKAALGRAGAAVIEPFLPMMKSGLRKVMEFADK VTPHLKRGAEKVSSGLSDMGKAFMSSGESIDGPANKMERFGVKARTVVDNIRGVWSIL ADGKFAGEKMTFGLSEDSKVVDVLFKIRETAIGVFDILFKGNYTSPIWGQLEDSKAVD VLFRIRESAKALWDVIRSPSGDKFTKFLETVKGSGDGAAGAMGSVRDGANGLTGALKK IGAAAAGGAAAIVSLGGDTATVAVTGIKALGNVMSFFADHTGLATVAIAGFAASVAIA KTVHVGYEAARIAQAVMMPAQMVIQRQMTLALIAHTAALRANIVAMGGALPVEQASLA SRTRLAVATRLSTAATVSSTSSLAAYAVAQRAAAASSGMLIGGMRQATASAAMMGARV GAVGTSALGGLKAGLGSAVGFLGGPMTLGIMAAAGAAMYMGSKFSESADKVADSQRTY REYGEAVKKTNTDLREALDQSNGVVDKNVSNVMTQKIDDLRTSSQKLADDAPGLWGYF GAAAADAWDHLDGQLDGTTYKSMKTADALSFDGERIISAMNKTGLSSSELGALVSGSQ ADFNKFSSGLQQAGGDSLFLNLKMQDMRAEFLHSQTAAGQVEAAIQQIGDKSVGASAG IDLMKNAYTQFINDSRTTEEAQKSVTIALDGVTQALANGGAAAMDASGKLDLTTKAGG QVDDAMKRMGAAWSEAGTAARTAATEQKLSAEEVEAAQRAAEQGVRDQFIQTAAQITG NTEKAQALADVFFRWPPEAKTNIVVLDAEARGKVEQFLKDNTSHTIDVLVRTTMLNQT PAGLPPTVVPSSWEGLMPYANGGISNLPEQATIQPGRGAGLVQWAEGETGGEAYIPLA PSKRGRSTAILAEVAERFGMRLESFADGGVRAALDAARSVTGNAYQLGGTGPAGFDCS GFVGWLQQILMGVPPDLAAGNRLYTTHSLLGGALEGLVSGSGPAGTVFVVGASEPHMA ATLAGQPVEAGGAHGTSRIGAPAVGASDGQFPSRFHLPNELIAGGVEGAAGLAATGRE VEWTEKDQLDLESARVAVQQAKEARDKTYGSEKKSDADRQQADLRVQRAELKVRTMEQ KRDGTGISAMSDEPAPPLTGEMGEDAITMRNAEISLLDAQLARDKTYNDPESTSLDKE KADLQVYSAQNSLAETKKRIAEDADKSGTDGFSVKDRLKKFGSDLAGIAVDSFLEIIG FESRFLDIPLPEFKAPGTGSTVGPGTPLDILKSVLPSDLSSRVFPQSEIEGQLPVTPG APGWVEGWMKALPVGLDLKQATGGPLDMLLKAQGGKIPLHDEGGWLMPGLTMNLTKKP ERVLNPAQNVNLERIANLDTKALMPPVDSAADSWRPGVQITNQITVSNDRSQIRKFRE ENAMLLMQYGGAR" gene complement(11038..11421) /locus_tag="7S6_11" CDS complement(11038..11421) /locus_tag="7S6_11" /note="Predicted by MGA" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72810.1" /translation="MAYRITPAHSEKAKVQIEFEAEDGTIHTLNVRKWHYRPKAVVLE SLKWVTEQEALVKSGERKDEPTDLDLMVFTLGLVQPDLAPIVEQLSLGEQFEIWQAWT GAITADDTDTSGATAAPADEAPEAS" gene complement(11834..12172) /locus_tag="7S6_12" CDS complement(11834..12172) /locus_tag="7S6_12" /note="Predicted by MGA" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72811.1" /translation="MAFRITPAHDASIQIDFEVPIKGRQKPVTFSVPKIDYIAPETLE EYRRWFGVEVEANRATERNCTLKLLELTKARNYDLLAKLTDGELMEINRHWKDASRVD VGESSASSDS" gene complement(12297..12896) /locus_tag="7S6_13" CDS complement(12297..12896) /locus_tag="7S6_13" /note="putative gp14; Similar to NP_817987.1 [Mycobacterium phage Che9d] (BLASTp score : 94.4)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72812.1" /translation="MAVNVLNAYVGTPPIDGGVFFRAPLGTELPADALAELDPAFKDH GAVGEDGVTVAQTRDNTDIKMYGGKTFINVQTGYDEQVTITLMEDDNEAVLKTAFGDA NVESTPATAAHGLQKTIYHTADPLPISSFVVDGISGKKTKRYVIENGQVVSVAEIKDV HNNVTSRTLTVKTYTPTSAELKGGNVVEYRDNGVKTTTP" gene complement(12950..>13110) /locus_tag="7S6_14" CDS complement(12950..>13110) /locus_tag="7S6_14" /note="Predicted by MGA" /codon_start=3 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72813.1" /translation="LVDRIFREARGRLFAGGYITNWSKTSGPVNFPDPGTNLPRFQLT GDLTLLIQ" ORIGIN 1 cttagtgctg tgtgtgtgtg gtactgttgt tgtactatat ttgggtgaga gattagctgt 61 gtttgtgttt attgtgtttg gttatgggtt ttgtatcgtt ttgtggttgg catgatctat 121 gtgtctatgt ctacctcaat tgagtgtgca tggttgactg gagtcgcttg ttttgtgttt 181 tgttttgcaa gcagtcgacg gcatacgagt ttcaccttag tctcgttgtc tcggagatgt 241 gtataagaga cagccgtgta tccgtgacgt tcaagtgctc tgcgcagcac ccgggccacc 301 ttcttcgcgt cctctgattg caccttgacc tcaaggcgct gttgctcggc ctgcgcgtgc 361 tgctcggcgg cctcctgggc ctgccggcgc gcgtcatcga cgtccttact ccacggccac 421 ttcatggtgc ccgcccaacc acttcatgaa tcgactggct cgcgtgcgcg gccaactggg 481 atgcaacctt ctgttcagcg atcgtgtccg ccaacgtgcg gatcgtgtcc tggtccgctt 541 gctcgcgtcc ctgtgaatga ttgatcgcgt catccttgag gcgcatgatc tcgcggtgtg 601 ctggccccca gacaatccag ccgcgaacca gggcaagccc atgaagaagg gcgagcacga 661 tcaccacagt gaccacgctc gccccttccc acgctgaggg tgcgaggatc tgcatcactc 721 tccccgagga acgttcctgc gggtgtacgc ctcgagctcg tcgatctgct tgtcggtcac 781 gccgttcggg gtgaacttga cgcccacgac ggtgccgagc gaggcgagta acaggccgcc 841 aacgacgacc tggtcaggca ggttcacgcc aacggagatg accgtccaca gcaccgccac 901 gacagcagca accgcagtcg ccactgtgtt ggcgtacttc ttcaccagcg gctgctcggc 961 catcttggcg cgcagcacgt cgaggatcgg gttcgggttc atcacttgcc tgccttcggg 1021 tcgaatccct caatgttcag cttcgcgccg atgaccgcga gggcgtcgac gacggtgcgg 1081 ttgccgagct gcttccatcc cgggtacttg cccagttccg cgctgccggt gagctggtta 1141 cggatttcct gctcgatgtt catggagcct cctacggggg gcttaggggc ggctgggccg 1201 agcgctctag accagtcgcc acgattgtcg aagaagtctg cagagaagct gacgtgcatg 1261 tgactggtgt gtgggttgct gccgttgtag cggcgccagg cgccccagtc cgcgttggtg 1321 atccagttat tgaagatcag gtatccgcca ttgcgcaggc gtggatcgcc agctttgccc 1381 atgtcgaaca ggtagtccga cagcttctcg cagtcgactc cattgcgcgg gtcgtgcgtc 1441 aggtcgaatg ctcggaccac gccgatgcca ttccggacaa cccatggatt gtggtccgag 1501 ccctggcctt ggccggcgtg tgcaacgtcg ccgatcgttc cgtccgacgc cttgctgcgg 1561 ttcgggaacc gggcgttgat cgctgcccgg tactcatcca gcgcgattgc tgttctccat 1621 gccatgtcac attcctccgt tctccacgat ccgatatcca cccgcaagct gtagcgcgag 1681 atgatgattc ggctcgtcgg tgatgtatgt gcccggctgc ggtcggatat cggcggacag 1741 gctcacaccc tcttcggtgc cgagggtgac cgtcatctgg ccgggctgcc ccgcaacacg 1801 ggggcggtgg tagacgatca ggtggtcagt tccctggatc ggcggttcga ttcggtacag 1861 cgtcgccaaa ccggcgaagc caggcagtcc gtcgaccacc atttcggcag tagccactac 1921 ggcctcctag gcggttgtcg tgaagttcac ggtcgtggac agtccggaca cgttgtcgcc 1981 gatatcgact gcttgcacgc gcaccgcgta tgcggttgac gcggtgaggc ccacgaagtc 2041 gtgataggtc gactcgacga cggcataccg ggttccgttg aggtagagga tgtagttctt 2101 caccatcacg tcgtcgatcg acgcggccca ggtgacgcga gctgatgttc tggtgatggc 2161 cgatgcggcg agacctgtcg gcgtcgatgg tgggacaccg agagtgtctt cgacgtagac 2221 ggttacgaac accgcgccgc cggcaccatt tccgacgcca ccgccattcg cgccgccgta 2281 tccgccgccg ccacctgggg cggcaggcca cccgccgtga ccaccctttc cgccagtgcc 2341 gaacaggctt gtgttcgcaa cgccgccgcc gcccgcgctg ccggtgccga tctggccgac 2401 gccagcgctg aagccattgc cgccattaat cgaccccggg ctgccgcctg agccgcccga 2461 actgaagggt ccagcgctac cgtcgctgcc tgcgatgacg gacccggacc caccggaccc 2521 gccgcgcatc ttgtacgtac gggagccgct cccgtagttg gatggggttg caccagtggc 2581 cgaaccgaac gatccgaacg acgacgttcc cgccgtcgcc gtcgacccgc cggatgttcc 2641 acccccgatc gtcacggcaa ccgacgacgg gagggcctcg agcgcagcac ccgtgaagac 2701 gaaccgctcc caaccgccag agaacccacc ggcaccgccg gcgccactgg tgcccgaccc 2761 gttggttccc tggtagcccc ccgacgcgcc agacaggacg tccacgacga ccttgtaggc 2821 cgcgggatgt ttcgtccaca cgccgtactc cgagaaggtc cacaaaatcg cacgctcggt 2881 gccgacgacg atctcgtcga tgcggttgtc gaggcggaca acctcaccat tgaacagcgg 2941 gccggagatg aactcagcct tgaacctttc gtgagcttgg ttgaagccgc cgatactgcc 3001 gaacttcgac gccctccatg acgcctcgtc gatgccgttc agccccgaca ggtcgtcatc 3061 gaacgtgccg tccggatagt cgccccacgg gacggtcatg atccgccccc gtcctgcagc 3121 cctgcatcgt ccgggaactg ctcgcgtaga cgccgcagca tctcccgctt cacctccggc 3181 cggatcgcat ccagccgttc cgccagcaca tcacgctcag gttctggcgc atccgctggc 3241 acccaccggc caccgccacc catcatcacg tcctcggtgg cgtcaatgcg gtacttcttc 3301 tttggctgct cgcggtgccc gcatgatggg cactgcagca tcaggccgca gtcgatttga 3361 tgcgcggaca ccttgcgcca gaacggaatg ggccacacca ggtccgcgcc gatgacgtcg 3421 gggagcccga ggtaggtcca gaggaacacc tcctctgggt cactgaggtc gcacttgtcg 3481 atcgtgggca actctttgtc gcgcatcaga tcatccccat cgctttgagc cctgaggcca 3541 acttctggat tcgcttgagc acctgcccga atgcgtcgac tggatctttt gacccgagct 3601 tgatcgcgaa cgctggatcc acccctggtg atgcggtgag gcgtagtgac ttcacccgcg 3661 gcgcttcgat ccgccccgac cggtcgccct cggtttgcac gaggatgcgg ttgccgaggt 3721 cgaagtgccc gtagcctttc gcgccgacgt accaggggga gccgtcgatg atttccatgt 3781 cggcggtgta cttcgtctcg gtcgcacgga ccgcctcacg cagcacgagt gtcgccgaca 3841 gggtgtaggc ctgatcggcg ccctcgaccc acatctcgta cagcgagaag tgcgacagat 3901 gcgccgacct gttgaccagg tacaccgtca tgaacgcgag gatcgtgtcc tcgtagaacg 3961 gcttgagcag tgcatctgcg acgccgccga gtggcgggat cggcgcgagc gggatcgccg 4021 ccaaggcatc gccgagggcc tggacggccg ccgagatcac ctcattgacg cccggcattg 4081 aatgcccgcc gccggttaat cgatggccgc gaccgatctt gatcttcgcg gactgtgaca 4141 gcacacccgg cgacccggct gggtagaaga cgtacgggcc cttcggctcc gtcccgaggt 4201 agcccggcac ctgatactca ggaaccgcgg ggataccgac gattgcctgc tcgccgttgt 4261 cgatgggcag tgatccgccg ccgatgcctt cgaccagcga cgacacgaag atgcgcacca 4321 gctgcccgat gccgccaagg aaggtgcccg tcgtcgtcgg cgcgagtcca ccagagttgt 4381 tctcgaacca gatgacgagc gtgccgtacg agatctgcgc tccaggccac ggcagggcgt 4441 cgcccggcat gtagcgccgc caccgggtgg ttatgtcggc gtcctcgacc atcgcttccg 4501 aggcctcgtg gtacgtcttg aaccgcgaca gcgccaggcc tgtcacagtg ccccggctgg 4561 ccgccgtctc atagtccagc ggcgccagga cgatcggcca ctcgcggtag tcggcagccc 4621 cgtccgcgag cgggtccatc caccccatgc ccgatgggcg accatgagcg cgtgccagat 4681 tcaacttcag tgccgtgccg agagcccaat ccgtggggcc accaagcatg aacaccttga 4741 acggctggaa gcccgccggc gtggaaggcg tggcccacag gtgtcgccac ttgagctgct 4801 cgtagtcgga caggaagttc gcggtgacaa tcgactggcc gagatcgttc gtctccatcg 4861 tgacttcatc gaggaatccg ctccaacgcc tgccgacgta atcaaccgtg accaagacgt 4921 cgatcgtctc gccgcgatcc ttgcgaccct tgatatccca gaggtactga ccctcttcga 4981 agtcggcatc gatcttgacg atgcccggac cggtgtcatt ctcgacgtcc agccagtcgt 5041 actcgttctc gcagcggacc acatgctgca ggccgatgtt gccgtcccac agcttgatca 5101 ccggggcgat cagacggaac gcttccagcg aggcctcgcg tgcgcggaca gcggcaagac 5161 cgtccgcgca cgcctgctcg agcgcagtcg ccatctatct acctccatag aagttcgacc 5221 acagccgcgg ctggtgcagc tccgcccgag caccacccgc cggcgcgccc gtcaccgaga 5281 tcggcaactg cgtcttcggg gtgtgcggcg ggatggtgtg catgaagtag tagccgccac 5341 ccacctcacc catgacgttc gtgccattcg agctctgcat catcacgtgc atcggatcga 5401 ggttgatcgt cagcgtgccg tgcgtggatg tgaccggttt caaggggatc gaccgagacc 5461 cgaacaggcc gcccggagtc cgcttgcgtg gtcgccctgt ccacgacacg tccgggatcg 5521 tccacgtgcc ccgggtcagg acccatgcct ggaacatctc cacgtcagtc ggattggaga 5581 cagtgatcgt gcccgacccg gaagtgcccg acgtctccca atgggtgaca tccgtcttcg 5641 actcccagaa gggttgcgcg gcacgcaacc tgtagaaaac attcccgtac cgcagctgat 5701 ccgggtccat ccgcggggcg aactccggct cttgatgcat ctgcaccgac agcgtcctgt 5761 agtcgtcgcc ggactcgacc gcgatgcggg cgagagtgtc acccggccac cacggatcga 5821 ggtggtacgt gaagcatgac cgcagcagat cgtccgcctc ggtgaagctc atccggtcgt 5881 caccgcgaac atgaaatccc aactgcatat cccgcgtcgg gttctccacc cccgccagag 5941 agccgccccg ctgccggcgg gcccgcttcc actcagactc gacaggagcg tggtagaggc 6001 ccttcacctg gtcctcgccg agcacgaacg gcgacgcaat gtcgcccggc ccggaaacca 6061 ccgtttccgg gccgccgccc gcgccgagca cggagatcgt gaacgccatc taccttgcac 6121 ctccgtactg catcagcagc atcgcgttct cctcacggaa cttccggatc tgcgaccggt 6181 cgttcgacac cgtgatctgg ttggtgatct gcacacccgg acgccacgaa tctgctgcac 6241 tgtcaacggg aggcatgagt gccttcgtgt cgaggttcgc gatgcgctcg agattcacgt 6301 tctgcgccgg attaagaacc cgctcaggct tcttcgtgag attcatcgtc agacccggca 6361 tcagccagcc gccctcatcg tggagcggaa tcttcccgcc ctgcgccttg agcagcatgt 6421 cgaggggccc gccggtggcc tgcttcagat ccaagccgac cggcagggcc ttcatccagc 6481 cctcgaccca ccccggggcg cccggggtaa ccggcaactg cccctcaatc tcggactgcg 6541 gaaacacccg cgacgacaga tcggacggaa gaaccgactt caaaatgtcc aacggggtgc 6601 ccgggccgac agtcgatcct gtccccggcg ccttgaactc cggcaacgga atgtcaagga 6661 accgggactc gaaaccgatg atctcgagga acgaatccac cgctatgcca gccaggtcgg 6721 acccaaactt cttgagccgg tccttcactg agaacccatc ggtaccggac ttgtcggcgt 6781 cctcagcgat acgcttcttg gtctccgcca acgagttctg cgccgagtaa acctgcagat 6841 ccgccttctc cttgtcgagg ctcgtcgact ccggatcgtt gtatgtctta tctcgagcca 6901 actgtgcatc cagcaaggag atctcggcgt tgcgcatcgt gatcgcgtct tcgcccattt 6961 cgccagtcag aggcggtgca ggctcgtccg acatcgcact gattccggtg ccgtcacgct 7021 tctgctccat cgtgcgaacc ttcaactcgg cccgctgaac cctgagatca gcctgctgcc 7081 ggtcagcatc cgacttcttc tcgctgccgt acgtcttgtc gcgggcctcc ttcgcctgct 7141 gcacggcaac acgtgccgac tcgaggtcga gctgatcctt ctctgtccat tcgacctcgc 7201 gtcccgtggc agccaaccct gcagcgccct cgacgccacc agcgatcagt tcattcggaa 7261 ggtggaaccg gctggggaac tggccgtcgc ttgcgccaac ggccggcgca ccaatgcggg 7321 aagtgccgtg tgcgccaccg gcctcaacgg gctgacccgc caaggttgcc gccatgtgcg 7381 gctcactggc gccgacgacg aacaccgtcc cagcaggccc tgatcctgag accagacctt 7441 caagggcgcc acccagaagc gagtgcgtgg tgtagaggcg gttccccgca gcgagatccg 7501 gcggcacgcc catgaggatc tgctgcagcc acccaacgaa cccggagcag tcgaaccccg 7561 cgggcccggt gccaccaagt tgatacgcgt tgcctgtgac ggatcgggcg gcgtcgagag 7621 cagctcgaac accgccgtcg gcgaacgact cgagtcgcat cccgaaacgc tccgccacct 7681 ccgcgaggat ggcggtcgag cggccacgct tcgacggggc gagcggaatg tatgcctcac 7741 caccggtttc gccttcagcc cactgcacca ggccagcgcc gcggcccggc tggatcgtcg 7801 cctgctcagg caggttgctg atgccgccat tggcgtaagg catcagccct tcccagctgc 7861 tagggaccac cgtcggcggt agcccagctg gcgtctgatt gagcatcgtc gtccggacga 7921 gcacatcgat tgtgtgactc gtgttgtcct tcaggaactg ttcaaccttg ccccgcgcct 7981 ccgcgtcgag caccacaatg ttggtcttag cctcgggtgg ccaccggaag aagacgtcag 8041 cgagggcttg cgccttctcg gtattgcctg tgatctgagc tgcggtctgg atgaactggt 8101 cgcgcacccc ctgctcggcg gctcgctggg ccgcttccac ctcctccgcg ctcaacttct 8161 gctcagtcgc ggccgtccgg gcagcagtgc ccgcttcgga ccacgccgcg cccatcctct 8221 tcatggcatc gtcgacctgg ccgccagcct tggtggtgag gtccagtttt ccgctcgcgt 8281 ccatcgccgc ggcaccgccg ttggccaatg cctgcgtgac cccgtcgagc gcaatggtga 8341 cggacttctg ggcttcctcc gtcgtgcggc tgtcgttgat gaactgcgtg taggcgttct 8401 tcatcaaatc aatgccggcc gatgcgccga cggacttgtc gccgatctgc tggattgccg 8461 cctcaacctg acctgccgcc gtctgcgaat gcaggaactc ggctcgcatg tcctgcatct 8521 tcaggttcag gaagagcgaa tccccgcccg cctgttgcag ccccgacgag aacttgttga 8581 aatccgcttg agagcccgac acgagcgcac cgagttctga actcgacagg cccgtcttgt 8641 tcatcgcgga gatgatgcgc tcaccatcga atgacagagc gtcggccgtc ttcatcgact 8701 tgtacgtcgt gccgtcgagc tgtccgtcga gatggtccca agcatcggct gcggcggcgc 8761 cgaagtagcc ccacaagccg ggggcatcgt ccgcgagctt ctgcgacgag gtgcgaagat 8821 catcgatctt ctgcgtcatc acgttggaga cgttcttatc gaccacgccg ttcgactgat 8881 ccagcgcctc acggagatca gtgttggtct tcttgaccgc ctcgccgtat tcccggtagg 8941 tgcgctgaga gtcggcgacc ttgtccgcgg actcactgaa cttcgagccc atgtacatcg 9001 cggcgccagc ggctgccatg attcccaacg tcatcgggcc gccgaggaac cccacagcac 9061 tgccgagtcc agccttaagg cctcccagcg ctgacgtgcc cactgcccca actcgggcgc 9121 ccatcatggc ggcgctcgca gttgcctgcc gcattccgcc gatgagcatc cccgatgacg 9181 cggcagcagc ccgctgcgcg accgcatacg ccgccagcga ggatgtcgat gacactgtgg 9241 cggctgtcga cagccgtgtc gccaccgcga ggcgcgtgcg cgaggcaaga cttgcctgct 9301 cgacaggcaa cgccccgccc atcgccacaa tgttggcccg caacgccgca gtgtgcgcaa 9361 tcagggcgag ggtcatctga cgctgaatca ccatctgcgc cggcatcatc acggcctgcg 9421 cgatacgcgc agcctcgtag ccgacatgca cggtcttcgc gatcgcgacc gacgctgcga 9481 atccggcgat tgccacggtc gccaacccgg tgtggtccgc gaagaagctc atcacgttgc 9541 cgagggcctt gatgccggtg accgcgacgg tggcggtatc gccacccagc gagacgatcg 9601 ctgcggcacc gcccgcggcg gccgcgccga tcttcttcag tgcgccggtc agtccgtttg 9661 cgccgtcgcg gaccgaaccc attgcgcccg cggcgccgtc gcctgaaccc tttacggttt 9721 caaggaactt ggtgaacttg tcgccagacg gggaccggat gacatcccac agagccttcg 9781 cgctctctcg gatccggaag agaacatcga cagccttgga gtcctcgagc tggccccaga 9841 tggggctcgt gtagttgcct ttgaacagga tgtcgaagac gccgatcgcg gtttcgcgga 9901 tcttgaaaag gacgtcgaca accttcgagt cttcgctgag tccgaacgtc atcttctcgc 9961 cggcgaactt gccgtccgcg aggatcgacc acacgcctcg aatgttgtcg accaccgtgc 10021 gggccttgac gccgaagcgt tccatcttgt tcgccgggcc atcgatggac tcacccgacg 10081 acatgaacgc cttgcccata tccgagaggc cggacgacac tttctcggct ccgcgcttga 10141 ggtggggggt caccttatcg gcgaactcca taaccttgcg gagtccggac ttcatcatcg 10201 gcaggaacgg ttcgatcacc gcggcgccgg cacgcccgag ggcggccttc atgttctcga 10261 acgagcctcg cagggtcttg ccggattcga gggctgcgcc gccgatattc ttctcgatga 10321 ccttgttgaa ggtctcggca tcgaccttgc cttccttcac catcttcgaa aggccgtcag 10381 ctgagacctt gtactcatcc tgcagccact gaaagatcgg gatgccacga tccgcaagtt 10441 gattcaggtt gtcggtgtat gccttgcctg atgtctgaac tttgttgaag atcgagccca 10501 tctcgcctag cgacgccccg gcgattgtcg ccgcgtcacc ggtcagcttc aggtacttgg 10561 tgagatcctg gccaggcttg atgcccgcgg cgacagcgga tgcggcgata cctgcggcgt 10621 cgccgagccc gaacgctgtg cccttcacgg acgccaacgc ggaatccatg atggtggtga 10681 caccttcggc ggtgtgaccg agaccggcga gctttccctt cgcgtcgtcg atcgcgacga 10741 cgcgcttcat gccctgagtg agagcggtgc cgatgacgcc ggcggccgcc gcgccggcgg 10801 tggctgcacc gaacttcagg gtcgacccga tgcccgatgc gagcttcccg ccgatgccgc 10861 gaccagcctt gtcggcgccg ccctgcgcgg aggtgagcgc agagttgatg cccgggacga 10921 ctttcgaagt ttccgggacg atcgacaggt atccgacgcc gagttcgaca ccaccggcca 10981 ctgggcacct ccactatgtt gttgtgctgc tgtgttgttc gcgaatgagt gggtcgatca 11041 ggaggcttcg ggggcctcgt ccgcgggcgc tgctgtcgcg cccgaggtgt ccgtgtcgtc 11101 ggcggtgatc gccccggtcc atgcctgcca gatctcgaac tgctcaccaa gggacagctg 11161 ctcgacgatc ggcgcgagat cgggctgcac cagcccgagg gtgaacacca tcaggtcgag 11221 gtcggtcggc tcatccttgc gttcacccga cttgacgagg gcttcctgct cggtgaccca 11281 cttgaggctt tcgaggacga cagccttcgg tcggtagtgc cacttgcgga cgttcagcgt 11341 gtggatcgtg ccgtcttcgg cctcgaactc gatctgaacc ttggccttct cggaatgggc 11401 gggagtgatt cggtatgcca tgggtgtgca ccaggccttt caggggttga gtgatgtcac 11461 gcagctttgg ctgcacggcg gcgcgcgagc tcgtcgcgga tctgagtggc gctcatcttc 11521 ggagtgctcg gagcctcatc gactgccgcc ttgacgggtg gcaggagcgg ctccggtcgc 11581 ggagtgttcg ggttccgtgc gcgctgccag ttcgcatcct ggagtgcgtc gtgagcggcc 11641 gcggcgatct gaacttcggt ggtccgccac catgtatccg ggttctgctg ccggccgatc 11701 gccgaatcgg agggcagcca acgaatcagg cacagcaggt cccaccacga cagacgttca 11761 ccgccaagat cgttcaggga cttgccgcgg tcgatgaggt cgcactggat ggcttcgcaa 11821 gcctcctcgt tctctaggaa tcggaggagg ctgaagattc ccccacatcc acccgggagg 11881 cgtccttcca gtggcggttg atttccatca gttcgccgtc tgtgagcttc gcgagaaggt 11941 cgtagtttcg agcctttgtg agttcgagaa gcttgagggt gcaattgcgc tcggtggcgc 12001 ggtttgcctc gacctccacc ccgaaccacc gtcgatactc ttcgagcgtc tcgggggcga 12061 tgtagtcgat cttcgggaca ctgaacgtga ccggcttctg ccggcccttg atcgggacct 12121 cgaagtcgat ctgaattgac gcgtcatgcg ctggggtgat gcgaaacgcc atgggtgtgc 12181 accaggcctt ccaggattgg gggaatcggt gttgcgccgg gccctatggc ccacccgaca 12241 ggggcgggcc cggtgcactt ggggatgtga atcacacccc tgccgggtga gttcagttac 12301 ggcgtggtgg tcttgacgcc gttgtcgcgg tactcgacga cgttgccgcc cttgagttcc 12361 gcactggtcg gcgtgtacgt cttgacggtc agcgtgcggg aggtgacgtt gttgtggacg 12421 tccttgatct ccgcaaccga gacgacctgc ccgttctcga tgacgtaccg cttcgtcttc 12481 ttgccggaga tgccgtcgac cacgaacgag ctgatcggca gcggatcggc cgtgtggtag 12541 atcgtcttct gcaggccatg cgccgcggtc gccggcgtgg actccacgtt ggcgtcgccg 12601 aacgcggtct tcaggacagc ctcgttgtcg tcctccatca gagtgatggt gacctgctcg 12661 tcgtagccag tctggacgtt gatgaacgtc ttgccgccgt acatcttaat gtcggtgttg 12721 tcgcgggtct gcgcgacggt gacgccgtct tctccgacag cgccgtgatc cttgaatgcc 12781 ggatccagct cggcaagcgc atcggcggga agctcggttc cgagcggtgc gcggaagaac 12841 acgcccccgt cgatcggggg ggtgcccacg tacgcgttga gaacattcac agccatttga 12901 tttgcccctt tcaggcgacg tgtgcaccgg gccaagaagg ggggtgaagc tactgaatga 12961 ggagcgtgag gtccccggtc agttggaatc ggggcaggtt cgtgcccgga tcgggaaagt 13021 tcaccgggcc tgatgtttta gaccagttgg tgatgtatcc gccggcgaac agtctgccgc 13081 gggcctccct gaagatacgg tcgacgagga //