LOCUS MF417977 12395 bp DNA linear ENV 27-FEB-2018 DEFINITION Uncultured Caudovirales phage clone 3F_2, partial genome. ACCESSION MF417977 VERSION MF417977.1 KEYWORDS ENV. SOURCE uncultured Caudovirales phage ORGANISM uncultured Caudovirales phage Viruses; dsDNA viruses, no RNA stage; Caudovirales; environmental samples. REFERENCE 1 (bases 1 to 12395) AUTHORS van Zyl,L.J., Abrahams,Y., Stander,E.A., Kirby,B.M., Clavaud,C., Farcet,C., Breton,L. and Trindade,M.I. TITLE Novel phages from South African skin metaviromes JOURNAL Unpublished REFERENCE 2 (bases 1 to 12395) AUTHORS van Zyl,L.J., Abrahams,Y., Stander,E.A., Kirby,B.M., Clavaud,C., Farcet,C., Breton,L. and Trindade,M.I. TITLE Direct Submission JOURNAL Submitted (29-JUN-2017) Biotechnology, University of the Western Cape, IMBM, C2, Life Science Building, Robert Sobukwe Rd, Cape Town, Western Cape 7550, South Africa FEATURES Location/Qualifiers source 1..12395 /organism="uncultured Caudovirales phage" /mol_type="genomic DNA" /isolation_source="healthy skin from forearm of human male" /db_xref="taxon:2100421" /clone="3F_2" /environmental_sample gene complement(<1..171) /locus_tag="3F2_1" CDS complement(<1..171) /locus_tag="3F2_1" /note="Predicted by MGA" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72830.1" /translation="MPLITRVFSGFAGSSPKAAAEAYPAASWLAVEAVPGSTGMEIAP EFNVLARVVAAES" gene 59..634 /locus_tag="3F2_2" CDS 59..634 /locus_tag="3F2_2" /note="Similar to YP_007002946.1 [Haemophilus phage SuMu] (BLASTp score : 139). Similar to PF04984.9 [Phage_sheath_1] (hmmscan score : 102.7)." /codon_start=1 /transl_table=11 /product="putative tail sheath protein" /protein_id="ASN72831.1" /translation="MLPGTASTASQLAAGYASAAAFGEDPAKPLNTLVIKGIKVPPIA SRLGRTEQETCLANGVTPLEVGPGDTVQIVRAVTTYTKAASGADDVALLDLTTIRTLY YVRQSSRDRVRLRFPRAKLSARTPAAVRGELLDVLKKLEELEIVENVDDNADALIVER STQDPNRLNASIPVDIVNGLHVFAGRIDLLL" gene 646..1017 /locus_tag="3F2_3" CDS 646..1017 /locus_tag="3F2_3" /note="Similar to YP_007002947.1 [Haemophilus phage SuMu] (BLASTp score : 95.9). Similar to PB018971 [Pfam-B_18971] (hmmscan score : 117.2)." /codon_start=1 /transl_table=11 /product="putative tail tube protein" /protein_id="ASN72832.1" /translation="MSDIYVGQIVLSINGTDYDVKSVDDTLKTGRTVVKTMNRTGRPK GTAAGMEEYDLRVSVAIPKTGEPNWRAMVDAKLTIEPLDGGGKRQTWTGVSLVEMGSK YALEGEATRDLTLSALNYYEE" gene 1017..1385 /locus_tag="3F2_4" CDS 1017..1385 /locus_tag="3F2_4" /note="Predicted by MGA" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72833.1" /translation="MAMSNIDKRWDGLTITRELRVGVYYAGTRHKTFTLRVAMSGDLI AAQREHPVGPIQLVTLEVYRQQLLALGDIPAEALTTELLQVELAEVDLALIADADAEL EKKLPPPSAASPTGDESSTT" gene 1512..1778 /locus_tag="3F2_5" CDS 1512..1778 /locus_tag="3F2_5" /note="Predicted by MGA" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72834.1" /translation="MAQAKEFPLSKQEAQVLSEAWQSRRASALLDLSGPGMNAGFQED LVNAARRMGGYQGPPGEYGYGLNAAGMPVLRWTPEPTTEVSKAQ" gene 1819..4014 /locus_tag="3F2_6" CDS 1819..4014 /locus_tag="3F2_6" /note="Similar to PF10145.4 [PhageMin_Tail] (hmmscan score : 67.0)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72835.1" /translation="MSSDLRVALRINATSGNSRREIQAIERDLRQAGKNGAKALADES GKAGTALNKTGKEGAASFKIIRQAMRDAAAQGSNVFRHGVIQTQGELKQLGQAAKQAA NQSKGEFVRADREGIEPLRRSVERTEGSFRRLAQNGGRNLRMLKSLAAGVRTELDRVK RLGGSAQGQLAGLGLGFGAATGLVGSAALDRQLIRTQQTAGMTAAQREEWREEGFRIA KQYGLGREGVDSGFNTLIASGVEYGAAKKTADAIGQGTAVTGADAAILGKAVVAGASA FNIDLNKDGAALELLQKMTVAGRLGNAELENLADLFPKIGQSAQRAGMSISQALAFTE TLSTVEMQPERLGTLADSTLRVFGIKAYQEQVTKSTGVQFFDKSGSYRDPAAVLGDLK GKYDKLKTDKDRARFLGVTFKGMDQDTVRGMQIMLSGNRLDTFKEQSAEIAKAEPVFN KDLADNVNSATGTGSRMRATLSQAIDRMAQPLNKSFTDFGTYILDDLNLTGEQMLGAG IATGLGGYYAGRGAKAGAGALLNKFLGGPETLKNVAVGKVLEEAAGVTSVFVTNWPAG GMQAPTPEVSKESKNWGRYAALGSAALTMSVLGGSSDNTDDARLRHAQASSLLTAGQK SYYTAFYRNRMSLAGANPTQSDDWVSQQAQRLAQQETGLTANGMSAAGGSAWASGVAN RAVEAGLNGVVQKLTALLDKPLVVEVRADTDYIHAQVERKAGIQVRRGQ" gene 4011..5336 /locus_tag="3F2_7" CDS 4011..5336 /locus_tag="3F2_7" /note="Similar to YP_007002951.1 [Haemophilus phage SuMu] (BLASTp score : 124). Similar to PF07157.7 [DNA_circ_N] (hmmscan score : 75.7)." /codon_start=1 /transl_table=11 /product="putative DNA circulation protein" /protein_id="ASN72836.1" /translation="MSWKDDLLDCTYRGVAMDIMGDDLEAPRAVVQHGVPYVNGDDVE DLGKEARVFSMRAVVFGPNYLLQLQKILVALDTLGPGELVHPIYGSVTVVEGGYKVSH DANRPDYAEIALRFLEHTPNDAFFNRDLIYTDTGTAYVEDERTWQDGVFDLIGRVDSL VAEVQQWIGGGWTGLMEKALGLPGITLRLQQMRSQIMGVVSGVESMADSAGSAFDPLI DLTRSPTEIRSSIEDSTPSASADLLSRTGLPSAMPGAESLPPDIARIGTAFLVTAREG ADPDGSALPTAMPDDPIEAVAFALITLVITELALSYATAIGVAIENDADDLALSPNDL ERLANLARSLIQAAILLHRHLYGIEEALPIIEGLRTTAALIQARARQLVLQRPPLLSR TVETSTNLRLLAHRWYSDSRRADELMRLNPALRSPYSIAPGTVIRAYAN" gene 5326..6453 /locus_tag="3F2_8" CDS 5326..6453 /locus_tag="3F2_8" /note="putative Mu-like prophage tail protein gpP; Similar to YP_007002952.1 [Haemophilus phage SuMu] (BLASTp score : 235). Similar to PF05954.6 [Phage_GPD] (hmmscan score : 61.0)." /codon_start=1 /transl_table=11 /product="putative tail protein" /protein_id="ASN72837.1" /translation="MQTEESIRLSIGGKIYKTWDGWSVESDLLTPADGFELELFTRDT VQLPEALVEGAACTLTLGRDQVLTGQLDEIEHDVTKRGIFIRVTGRDKAAVLVDCSAP FVSMREASLAQILKEVVTPLGIAKVEVRAAGKPIRRRVQVEPGQSGWEALLQVAEANG LWPWVEPDGLLVVGGPDYTSTPVGELILRTDGTGNNVERLTVRRSIAGRYSQVTVLGQ HGQYDNDGLDTAGKTHMRSVIKDDVLARRGIFRPKIIIDSASESQDMATTSARKVLSD SRLEGFEIRAVVKGFRAPNGAVWKPGQRVIVRSEPHGLDATYFIMSRTLRLTRGQGAY TELRMREDKMWVLDGNPLKKHKGKKSKTDADASFIQMIRSL" gene 6453..7013 /locus_tag="3F2_9" CDS 6453..7013 /locus_tag="3F2_9" /note="Similar to YP_007002953.1 [Haemophilus phage SuMu] (BLASTp score : 100). Similar to PF06890.7 [Phage_Mu_Gp45] (hmmscan score : 107.4)." /codon_start=1 /transl_table=11 /product="putative baseplate assembly protein" /protein_id="ASN72838.1" /translation="MGSIPGLVREQVERAMRGVRQAFRAIGTRNTHGPSIGVQMQGLA GETVVGELAQHYGYTSAPLAGAEFIALPIGGNSKHVVVIATEDARYRLKIKDGEVALY TDEGDHVHLKRGRVIEVETETLLVKASKKVRFETPLIEASGEYQGEGNIQSQADVIDR VRSMQADREIYNGHQHGNSPTPTQQQ" gene 7075..7446 /locus_tag="3F2_10" CDS 7075..7446 /locus_tag="3F2_10" /note="putative Mu bacteriophage protein gp46; Similar to YP_007002954.1 [Haemophilus phage SuMu] (BLASTp score : 113). Similar to PF07409.7 [GP46] (hmmscan score : 71.8)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72839.1" /translation="MREATCLAMDASINPTTGDLTGQRITTLANAVYLRLMTPLGSYW ADPELGSRLHELKREKDKARVSTLAIQYAQQALQGLIDDGRATSVEVTAEQPHNGWLR LLIEVYAPAGRQTFEHLVSVI" gene 7446..8516 /locus_tag="3F2_11" CDS 7446..8516 /locus_tag="3F2_11" /note="putative baseplate J family protein gp47; Similar to YP_007002955.1 [Haemophilus phage SuMu] (BLASTp score : 196). Similar to PF04865.9 [Baseplate_J] (hmmscan score : 137.9)." /codon_start=1 /transl_table=11 /product="putative baseplate J family protein" /protein_id="ASN72840.1" /translation="MPYEAPPFDAIRSRALRDVRSQLPEADITSDSDNHVRASSVAAV AEGIHQQASWTARQIFPDSADETELKRHAATRNVFPKTATPAGNSLELTGGANAPVAA GLRVRHVASGQIFATVGSVVIPASGVATVLVTSEETGSALNGLEGPCVFISPPLNVDS ACTLTATVGGTDDESPESLLARYLDVLRNPPSGGSIADYRRWALSVNGVSTVLILPKR RAGNAIDVVITSAGGASSDAVIQACQAFIESVAPAGADIWVFTPQIVEVHIRARVKPA PNFTIESLQSPTEDACKKVIEPIAPLETLYLIRLSSAMSALGGVVDLVIEAPTGNVIT SSDPSLVQWARFGSVQLLPLEG" gene 8519..9124 /locus_tag="3F2_12" CDS 8519..9124 /locus_tag="3F2_12" /note="Similar to YP_007002956.1 [Haemophilus phage SuMu] (BLASTp score : 80.1). Similar to PF10076.4 [DUF2313] (hmmscan score : 106.3)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72841.1" /translation="MSEKIVEQLQSLLPPVSYDPQGKVLLTQIKAEVNALNQALTQLE EVEAAIFPESAGRYIADWERVYQIVPPPGATQDQRVQAVLAAMGDLGGQSIPYFIRLA SLFGVNVDIDTFRVPVVGLLDTGQPLYSGDWPFTWRVNAPLKDYTAPVMETRLTDRRP ANTEVVFGYGKEVVDGVTAAVDQFFNSIHYVIPSSLSKLNG" gene 9117..11441 /locus_tag="3F2_13" CDS 9117..11441 /locus_tag="3F2_13" /note="Predicted by MGA" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72842.1" /translation="MAEQTIEALQSYAGQLSEAATQAKAAASVQHQVVHGGPTQDVLT EGGLVPSLAKQAVLGQQQVATVLENVATQLAGATIYSTVAKGLLATVNGAYFSVRSAV PYLYVDLYLNNNGTAVYETSYLSNQADSITLNKGKAFPFKQMTRAGVTSVASTVLNNF ILNVKIIGDDAALEGKYYRIAYFQNDATIGSNADQGIVIEQFDAATYASTGVATTIHN HTDAPAGIIRNGGVQTFVIAPASMPTVRFVITVDATSLPAAGTPINALTSALAHYSWI IDPSCHVRVLTIGDSLTVNKGKVYPLRRMTRNTIVSAEHPSFSRAILDIKVRGARPGK FYRLAYYVNGSTALPTAKPDGWIVEEIDQANYATADNPYTQVIRLADDSTPTILRDGI QTVHLVSSVVLGLTVTITLDTAELPALGGYISAVQTYNPGYSFIIDPATYTMASSGAA GTAPLTYQVGAGGRITIVWDDGDIPRGFTFGPTGQNNLPNFAELLRDGVVIGVYGTDW LPPLLFDVKRNGDSSASLNFTGGNHLVDDLKTGVNIQYDIEADGVPLVQGDSGRADRI TCRVVNKVIAGNAVHLSRYSVIQSLHLDFAPGACNVHAEVMALEDVQFYIDYGCQMVA GAVNDTLFYLGGQLSEPVAFDSTLTSGSPSVYPNAWAVLCTSANGQLAAWIDRGYGVA DGTQVSEAYGMIIGGGGASTKQYTTAIHRFLQRNPDNPNYMELPSGKSYKWRGGYSWG PVDVKPGFVASLRYLDNGRMRRANAINGTLIVNP" gene 11485..12306 /locus_tag="3F2_14" CDS 11485..12306 /locus_tag="3F2_14" /note="Similar to YP_006987667.1 [Cronobacter phage vB_CsaP_GAP52] (BLASTp score : 97.8)." /codon_start=1 /transl_table=11 /product="putative structural protein" /protein_id="ASN72843.1" /translation="MTEFMQRISAFTDQCTPQGLFRYGTTAGGVPPTPVKAEFLNLLQ EELCNLVLAYLPALDANDNTQLLKAVRKLVLNYYTKPETDDLLATLKQTIQGLLDLKA PLASPVFTGTPQVPTAAAGTKTKQAASTEFVQAALAGAYPVGSIYINGSSSTNPATLL GFGVWQAAGTGRVLIGAGSGTDARGEAKSFTGGATGGEYNHVLTVSEMPEHAHPMPQG SVAPSGTTGPVYASGDDVTRTTIGSETPDTGKIGGRAAHNNLQPYLVVYMWQRTS" ORIGIN 1 gctctcggca gccactaccc tcgccagtac gttgaactcc ggtgctatct ccatacctgt 61 gctgcccgga accgcctcaa ctgccagcca gctcgctgcc ggatacgcct cggcggctgc 121 ttttggggaa gatccggcga aaccgctgaa caccctggtg attaagggca tcaaggtgcc 181 gccgattgcc agccgcttgg ggcgtaccga acaggaaacc tgcctggcta atggtgttac 241 cccgcttgag gtaggtccgg gcgataccgt gcagatcgtg cgagcggtga cgacttacac 301 caaagcagca tcgggggcgg atgacgtggc gctgctcgat ttgacgacta tccgcacgct 361 ctactacgtc cgtcagtcca gccgggaccg cgtccgactg cgcttcccgc gcgccaagtt 421 gtccgcccgt accccggccg cagtgcgcgg tgaactcctg gacgtactca agaagcttga 481 ggaactggag atcgtcgaga acgttgatga caacgctgac gcgctgatcg tcgagcgttc 541 gacgcaggat ccgaaccgac tcaacgcatc gatcccggtc gatatcgtga atggcctgca 601 tgtgtttgcc ggtcgcattg accttcttct gtaaccggag cccccatgtc agatatctat 661 gttggccaga tcgtattgtc gatcaacggc actgactacg acgttaagag cgtcgatgac 721 accctcaaga ctggtcgcac cgtggtcaaa accatgaacc gcaccggccg gccaaagggc 781 actgcggccg ggatggaaga gtacgacctt cgcgtatcgg tagccattcc caagaccggc 841 gagccgaact ggcgcgcaat ggtagacgcc aaactcacca ttgaaccgct ggatggcgga 901 ggcaagcggc agacctggac gggcgtatcc ctggtcgaga tgggcagcaa gtacgcgctt 961 gagggtgagg caactcgcga cctcacgctg tccgctctga actactacga ggagtaatgg 1021 ccatgtcgaa cattgataag cgctgggacg gcctgaccat tacccgcgag ctgagggttg 1081 gcgtgtatta cgccggcacc cgacataaaa ccttcaccct ccgagtggcc atgagcggtg 1141 atctgatcgc tgcccagcgg gaacatccag tcggccctat ccagctggtg acactggaag 1201 tctaccgtca gcaactgctc gctctgggtg atatcccggc cgaggcgctg accaccgagc 1261 tgctgcaggt tgagctagcc gaggtcgatc tggccctgat cgctgatgcg gatgccgaac 1321 tggaaaaaaa gctgccgccg ccgagcgcgg cctcacccac tggcgacgaa tccagcacca 1381 cctagtccgg cacggctacc ggctagagga aatcagccag atgaccaagc cggagatcga 1441 tgcccgcgtt ggtcttctgg ttggtcgaaa cacacagggt aagagacggt tcgtctctat 1501 gaaggatcta aatggcacag gcaaaagagt tcccactcag taagcaggaa gcgcaggtgt 1561 tgagcgaggc atggcagtcc cggcgtgcat cggcgctgct cgatctgtcc gggccgggca 1621 tgaatgccgg cttccaagaa gacctggtca atgccgcccg ccgcatgggg gggtatcagg 1681 ggccacctgg tgagtacggc tacggcctca atgctgcggg gatgccggtt ctacggtgga 1741 cccccgagcc gaccaccgaa gtatcaaagg cccagtgatg ggcctttctt ctttttataa 1801 gctcgcggga gtgacctcat gagttccgat ttgcgtgtag ccctgcggat caacgccacc 1861 tcggggaaca gccggcgtga gatccaggcc attgagcgcg acttgcgcca ggcggggaag 1921 aacggtgcca aagcgctcgc tgacgaaagc ggcaaggctg gcacggcgct caacaagacc 1981 ggtaaggaag gcgctgccag cttcaagatc atccgccagg cgatgcgtga cgcggccgcg 2041 cagggctcca acgtcttccg ccatggcgtc atccagactc agggcgagtt gaagcagctt 2101 ggccaggctg ccaagcaggc agcgaatcag tccaagggcg agttcgtcag agccgaccgc 2161 gaaggcatcg agccgctgcg ccgcagcgtt gagcgtaccg agggcagctt tcgccggctg 2221 gcccagaatg gtggccgcaa cctgcgcatg ctgaagtcat tggctgccgg tgttcgcacc 2281 gagctggacc gtgtgaagcg cctgggcggt agcgcccaag gccaactggc cggcctcggg 2341 ctcgggtttg gtgctgccac cggtctggtg ggtagtgcgg ccttggatcg tcagttgatc 2401 cgcacccagc agacggccgg catgacggcc gcccagcgtg aagagtggcg tgaggaaggg 2461 ttccggatcg ccaagcagta cggcttgggt cgtgaaggtg tggacagcgg cttcaacacg 2521 ctgatcgcct cgggcgttga gtatggggcg gccaaaaaga ctgctgacgc catcggccaa 2581 ggcactgccg ttacgggcgc agacgcggcg atcctgggca aggccgtcgt ggccggtgcc 2641 agtgcgttca acatcgacct caacaaggac ggtgccgcgc tggagctgtt gcagaaaatg 2701 accgtcgccg gtcgcctcgg caacgccgag ctggagaacc ttgccgacct gtttcccaag 2761 atcggccagt ccgctcagcg ggcaggcatg tcgatctctc aggcactggc cttcaccgag 2821 accctgtcca cagtcgagat gcagcccgag cgcctgggca ccttggctga ctcgacgttg 2881 cgggtgtttg gtatcaaggc gtaccaggag caggtgacca agtccacggg cgtgcagttc 2941 ttcgacaaga gcggcagcta ccgtgacccg gcagcagtcc tgggcgacct caagggcaag 3001 tacgacaagc tgaagaccga caaggatcgg gctcgcttcc ttggcgtgac cttcaagggc 3061 atggaccagg acaccgtccg gggcatgcag atcatgctgt ccggcaaccg cttggacacc 3121 ttcaaggagc agtccgccga gatcgctaag gctgagccgg tgttcaacaa ggacttggcg 3181 gacaacgtaa acagcgccac gggcaccggc agccggatgc gtgccacgct cagtcaagcc 3241 attgaccgga tggcgcagcc gctcaacaag agctttaccg acttcggcac ctacatcctg 3301 gatgacctca acctgaccgg tgagcagatg ctgggcgccg gtatcgccac cggccttggc 3361 ggctactacg cgggtcgcgg tgccaaagcc ggtgcaggtg cgctgctcaa caagttcctt 3421 ggcggtcccg aaacactgaa gaacgtggct gtgggcaagg tgcttgagga ggcagccggc 3481 gttacctccg tgttcgtcac caactggccg gccgggggca tgcaggcccc gacgcccgag 3541 gtgtcgaagg aatcgaaaaa ctggggccgg tacgccgccc ttggctcggc cgcactgacc 3601 atgagtgtgc tcggtggcag ctccgataac accgacgacg cccgcttgcg ccacgcgcag 3661 gccagcagcc tgctgaccgc tggccagaag agctactaca ccgcgttcta ccgcaaccgc 3721 atgagcctgg ccggggcaaa ccctacccag tcggatgact gggtgtcgca gcaggctcag 3781 cgcctggcgc agcaggaaac cggcctaacg gccaatggca tgtcggccgc agggggcagc 3841 gcctgggcgt ctggcgtggc aaatcgggcc gttgaggcgg gccttaacgg tgtagtccag 3901 aaactgacag ccctgctgga taagcctctg gtcgttgagg tgcgggcaga caccgactac 3961 atccacgctc aggttgagcg caaggcgggt attcaagtga ggcgtggcca atgagctgga 4021 aagatgacct gctggactgc acgtatcgtg gcgtcgccat ggacatcatg ggtgatgact 4081 tggaagcgcc tcgcgctgtg gtccagcatg gtgtgccgta tgtgaatggc gatgatgttg 4141 aggacttggg caaggaagcc agggtgttct cgatgcgcgc cgtggtgttc ggtccgaact 4201 acttgctcca gctccagaag atccttgtgg ctctggatac cctcggcccc ggcgagctgg 4261 tccacccgat ctacgggagc gtgaccgttg tcgagggtgg ctacaaggtc agccatgacg 4321 cgaaccggcc ggactacgcc gagattgccc tgcgcttcct ggagcacacg ccgaacgatg 4381 ctttcttcaa tcgtgacctg atctacaccg acaccggcac cgcctatgtc gaggatgaac 4441 gcacctggca ggacggagtt tttgacctga tcggccgcgt ggattcgctg gtggccgagg 4501 tgcagcagtg gattggcggt ggatggactg gcctgatgga gaaggcgctg ggcctgccgg 4561 gcatcaccct gcggctacag cagatgcgct cgcagatcat gggcgtggtg tccggggtcg 4621 agtcgatggc cgacagcgcg gggtcggcct tcgaccccct aattgacctg accagatcgc 4681 caaccgagat ccgttcctct atcgaggaca gcaccccaag cgcctcggcc gacttgctga 4741 gccgtaccgg gctgccatcg gctatgccgg gcgcggagtc gctgccgcct gatatcgcac 4801 gcattggcac cgcattcctg gttacggcgc gtgagggagc agacccggac ggctcggcgc 4861 tgccaacagc catgcctgat gaccctatcg aggcagtggc attcgcgctt atcaccctgg 4921 ttatcactga actggccctg agctacgcca cggcgattgg ggttgccatc gagaatgatg 4981 cggacgattt ggcactcagc ccgaacgacc tggagcgttt ggccaatctg gcgcgcagtc 5041 tgatccaagc tgccatcttg ttgcaccggc acctgtacgg catcgaggag gcattgccca 5101 tcatcgaagg cctgagaacc actgcggccc tgatccaggc tcgcgcccgg caactggtgc 5161 tccagcgtcc gccgctcctg tccaggacag tcgaaacctc taccaacctg cggctcctgg 5221 ctcatcgctg gtacagcgac agtcgccggg cagatgagct gatgcgtctt aaccccgcgc 5281 tgcgttcccc ctacagcatc gctccaggaa cggtgatccg cgcttatgca aactgaagag 5341 tccatccgcc tctctattgg cggcaagatc tacaagacat gggatggctg gtcggttgag 5401 tcggacctac tcaccccggc cgatgggttc gagctggagc tgttcactcg cgacacggtg 5461 cagttgccgg aggcactggt tgaaggtgca gcgtgcacgc tgacgcttgg ccgtgaccag 5521 gtgctgaccg gccagctgga cgagatcgag cacgacgtaa cgaagcgagg cattttcatc 5581 cgcgtcaccg gccgggacaa agcggcggtg ctggtcgatt gctcggcgcc cttcgtctcg 5641 atgcgtgagg catctctggc ccagatcctg aaggaagttg tcaccccgct tggcattgcc 5701 aaggtggaag tgcgtgcggc gggcaagcca attcggcgcc gggtccaggt cgagccaggt 5761 caatcggggt gggaggcgct attacaggtg gcagaggcca acggcctgtg gccgtgggtg 5821 gagccggacg gtctcctggt agtaggcgga ccggactaca cctcaacccc agtaggtgag 5881 ctgatcctgc ggacggatgg caccggcaac aatgttgaac gactgaccgt tcgccgttcc 5941 atcgccggcc gctacagcca ggtgaccgtg ctgggccagc atggacagta cgacaatgac 6001 ggactggata ccgcaggcaa gacccatatg cgctcggtca tcaaagacga tgtgttggct 6061 cgtcgcggga tcttccggcc gaagatcatc atcgacagcg ccagtgagag ccaggacatg 6121 gccaccacca gcgcgagaaa ggtgctgtct gatagccgtc ttgaggggtt tgagatccgt 6181 gcagtggtca aaggcttccg tgcacccaat ggtgctgtct ggaagccagg ccagcgcgtg 6241 atcgtgcgca gcgagcccca tgggctggat gccacctact tcatcatgtc ccgcacattg 6301 cgtctgactc gcggccaagg cgcgtacacc gagctgcgga tgcgcgagga caagatgtgg 6361 gtgctcgatg gcaacccact gaagaagcac aagggcaaga agtcgaagac ggatgccgac 6421 gcttcgttca tccagatgat caggagtctg taatgggatc aattccaggg ctcgtccgcg 6481 agcaggttga gcgtgccatg cggggcgtcc gtcaggcatt ccgggccatc ggtacgcgaa 6541 acactcacgg cccaagcatt ggggttcaga tgcagggctt ggctggtgag acggtcgtgg 6601 gcgagctggc tcagcactac ggctatacgt cggcgccctt ggccggggcc gagttcatcg 6661 cactgcccat cggcggcaac agcaagcatg tggtggtgat tgctaccgag gatgcccgct 6721 atcgactgaa gatcaaggat ggggaggtag cgctttatac cgacgaaggc gaccacgttc 6781 acctcaagcg cggccgggtg atcgaggtcg aaaccgaaac gctcctggtc aaggccagca 6841 agaaggtccg atttgagacg cctctgattg aagcctctgg tgagtaccag ggcgaaggca 6901 acatccagtc gcaggcggat gtcattgacc gggtccgcag catgcaggcc gaccgcgaga 6961 tctacaacgg ccaccagcac ggcaacagtc cgactccaac ccagcagcag taacccgccc 7021 tctatttctg gtccatgtaa gcagctgtca tgggccagaa atacctaagt ccgcgtgcgc 7081 gaggcaacct gcctcgctat ggacgcaagc ataaacccca ccaccggcga cttgactggc 7141 cagcgcatta cgacgctggc caacgccgtt tatctccgcc tgatgactcc cctcggcagc 7201 tactgggccg acccggagct gggttcccgg ctgcatgaac tcaagcggga gaaggacaag 7261 gcgcgtgtca gtacgctcgc catccagtac gcccagcaag ccctccaggg gctgattgat 7321 gacggtcgtg cgacatcggt ggaagtcact gctgagcaac ctcataacgg ctggctgagg 7381 ctcttgatcg aggtctatgc cccggccggc cggcagactt tcgagcacct ggtgagcgtg 7441 atctgatgcc ctatgaagcc cccccattcg atgccatccg ctctcgggcc ttgcgtgatg 7501 tccgctcgca gttgcctgaa gcggatatta cgtctgacag tgataaccac gtccgcgcca 7561 gttcagtggc ggcggtcgcc gaaggtatcc accagcaggc ctcctggacg gcacgccaga 7621 tcttcccgga cagcgccgac gaaacagagc tgaagcggca tgccgcgacc cgcaatgtct 7681 tcccaaagac ggccaccccg gccggcaact cgcttgagct cactggcggg gccaatgccc 7741 cagtagcggc agggctgcgg gtacggcatg tggcctctgg ccagatattc gcgaccgtgg 7801 gcagcgtcgt cattccggca tctggagtgg ccacggtact tgtcacctca gaggaaaccg 7861 gctcggcgct caacgggctg gagggaccat gtgtattcat cagcccgccg ctcaatgtgg 7921 acagcgcctg caccctcacg gcgactgttg gcggcactga tgacgaatcg cccgagagtc 7981 ttctggcccg ctacctggac gtactgcgca acccgccgag cggggggtct attgccgact 8041 atcgccgctg ggcgctatcg gttaatggcg tatcaactgt cctgatcttg cccaagcggc 8101 gcgccggcaa tgcaattgat gtcgtgatta catcggcagg tggtgcttca tccgacgcgg 8161 taatccaggc gtgccaagcc tttatcgaaa gtgtcgcgcc ggccggggca gatatttggg 8221 tgttcacgcc gcagattgtg gaagtgcata tccgggcgag agttaagccg gcaccgaatt 8281 tcaccattga gtcacttcag tcaccgaccg aagacgcttg caagaaagtt atagagccta 8341 ttgcgccgct cgaaaccttg tacctgatcc ggctttcttc tgcgatgagt gcattgggtg 8401 gagtggtaga cctggtgatt gaggcgccca caggcaacgt gatcacctcg tctgatcctt 8461 cgcttgtgca gtgggcgcgg tttggttcgg tgcaactgct gccgctggag ggttagaagt 8521 gagcgagaaa attgttgaac agttgcagtc actactcccg ccagtctcct acgaccctca 8581 gggcaaagtg ctgctcactc aaataaaggc cgaggtcaac gcgcttaatc aggcgctgac 8641 ccagctggaa gaagttgaag cagcaatatt cccggagtct gccgggcgct atatcgccga 8701 ttgggagcgt gtctaccaga ttgttccgcc tcctggtgcg acgcaagatc agcgtgtgca 8761 ggccgtattg gctgccatgg gtgacttggg tggtcagtcg attccttact ttatccggct 8821 ggcgtccttg tttggcgtga atgttgatat cgacacattt cgtgtgccag tagtcgggct 8881 gctcgacacg ggccagccgt tgtattcggg cgactggccg ttcacttggc gagtaaacgc 8941 gcctcttaaa gactacacag ctccagtaat ggaaacccgt ctcacggatc gcaggccagc 9001 caatacagaa gttgtgtttg gctacggtaa agaagtcgta gatggtgtga ccgccgctgt 9061 cgatcagttt ttcaactcta tccattacgt gatcccttcc agtctgagta aattaaatgg 9121 ctgagcaaac tatcgaagcc ctgcaaagct atgcggggca gctttccgag gccgcaactc 9181 aggcgaaggc ggcggccagt gtccagcacc aggtggtgca cggcggtcca actcaagatg 9241 tacttacaga gggtgggcta gtcccctcat tggccaaaca ggccgttttg ggtcaacagc 9301 aagtagcgac tgtgttggag aatgtcgcga cgcagctggc aggcgctact atctacagca 9361 cggtcgcgaa gggcttgtta gctaccgtaa acggtgctta tttcagcgtg cgctctgcgg 9421 tgccgtactt gtatgtagac ctgtacctca acaacaacgg tacagcggtt tatgaaacca 9481 gctacctgag caaccaggcc gactcgatca ccctcaacaa gggtaaagcg tttccgttca 9541 aacaaatgac ccgagctggt gtgacttcgg tcgccagcac tgtgctgaat aacttcattc 9601 tgaatgtaaa gatcattggc gatgatgcag cacttgaggg caagtattac cgcatcgcct 9661 atttccaaaa tgacgcgacc atcggcagca acgctgacca gggcattgtg atcgagcagt 9721 ttgatgcggc cacttatgcc agcactggtg tcgcgactac gatccacaac cataccgacg 9781 ccccggccgg gattattcgc aatggtgggg tgcaaacctt tgtaatcgcg ccggcttcga 9841 tgcctacggt gcgctttgtc attactgtgg atgctacgtc tctaccggca gccggcacgc 9901 cgattaatgc cctgaccagc gcactggcgc actattcgtg gattattgat ccatcttgcc 9961 atgtacgcgt gctgaccatt ggcgactcgc tgacggtcaa taagggtaag gtttatcccc 10021 ttcgcaggat gacccgtaac actatcgtca gcgctgaaca tccgtcattc agtcgcgcaa 10081 ttctagatat caaagtgcgg ggtgcgaggc cgggtaagtt ctatcgtctg gcctattacg 10141 tgaacggcag tacggcactc ccaacagcga aacctgatgg ctggattgtc gaggaaatcg 10201 accaggcgaa ctatgccacg gccgacaacc cttataccca agtaatcagg ctggcggacg 10261 atagcactcc gacgatcttg cgtgacggca ttcaaactgt gcacctggtc agttctgtgg 10321 ttttgggttt gacggtaaca atcacgctgg atacggccga gttgcctgcg ctgggcggct 10381 atatctcggc ggtgcagacg tataaccccg gttacagctt catcatcgac ccggccacgt 10441 acaccatggc ttcgtctggt gcggctggca ccgctccgct gacctatcag gttggtgctg 10501 gggggcgcat tacaattgtt tgggatgacg gggatatacc tcgtggcttc acgtttgggc 10561 ctactgggca aaacaatcta cccaatttcg cggagctgct tcgtgatggg gtagttattg 10621 gggtatatgg cactgactgg ttgccgccat tattgtttga cgtaaaacgt aatggtgaca 10681 gcagtgcctc gctcaacttt actgggggta accacttggt ggatgacctc aaaactgggg 10741 tcaacatcca atatgacatc gaggccgatg gcgttccgtt ggtgcagggg gatagcggtc 10801 gcgctgaccg catcacttgt cgcgtagtca acaaggtaat tgcgggtaac gcggtgcatc 10861 tgtcgcgcta ctcggtaatc caaagcctgc atttggattt tgctcctggt gcttgcaacg 10921 tccacgctga ggttatggcg ctggaagatg tgcagttcta tatcgactat ggctgtcaga 10981 tggttgcggg tgcagtcaac gacactttgt tctacctcgg tggccagttg tctgagcccg 11041 ttgctttcga cagtacgctg acttcgggtt cgccttcggt ttacccgaac gcctgggcag 11101 tactttgcac cagtgccaat ggacaactgg cggcatggat tgatcgtggt tatggcgttg 11161 ctgacggcac gcaagttagc gaggcctacg gaatgatcat tggtgggggc ggagcttcga 11221 ccaagcaata caccacggct attcaccgat tcttgcagcg caaccccgat aaccccaact 11281 acatggagtt gccttcgggc aagagctaca agtggcgagg tggttacagc tgggggcctg 11341 tcgatgtaaa gcccggcttt gtggcgtcac tacgctacct ggacaacggc aggatgcgcc 11401 gggcgaacgc aatcaacggg acactgatag tcaatccctg acggcttttc agcttcgttt 11461 aaggccttcc agccctcaca atgagtgaca gagtttatgc aaagaatctc cgcttttacc 11521 gaccagtgca cgcctcaggg gctgttccgt tatggcacga ccgctggtgg tgtgcctccc 11581 actccagtta aggctgagtt cctgaacctc cttcaggagg agttgtgcaa cctggtattg 11641 gcctacctgc cagcgctgga cgcaaacgac aacacgcagc tgctcaaggc cgtccgaaag 11701 ctggtcctga actattacac caagcctgag acagacgacc tccttgcgac tctcaagcag 11761 accatacagg gcctgctgga tctcaaggcg ccgcttgcca gtcctgtgtt cacaggtacg 11821 ccccaagtgc ctacagcggc ggctggcacg aaaaccaagc aggccgcgag cactgagttt 11881 gtgcaggccg cgctcgccgg tgcctaccct gtgggcagca tctacatcaa cgggtctagc 11941 tcgaccaacc cggctaccct gcttggcttt ggcgtctggc aagcggccgg gaccggtcga 12001 gtgcttatcg gtgcgggtag tggtacggac gcacgggggg aggctaaatc gttcactggt 12061 ggggccacgg ggggtgagta caaccacgta ctaaccgtga gcgagatgcc agagcatgct 12121 cacccgatgc ctcagggcag tgtggcgcca tctggcacta cagggccagt gtatgcgtct 12181 ggtgatgacg tcactcgcac tacgattggc tcagagacgc ctgatacagg caagatcgga 12241 ggcagggccg cccacaacaa cctccagccc tacctggtcg tttacatgtg gcagcgcact 12301 tcctgagggc tggtgccaaa gaccgcgcca aggtcgtcgg ggtgctcgat gccaaagaga 12361 gcgctcggcg gtgccaaatc cggcgcgcgc ctaca //