LOCUS MF417980 6955 bp DNA linear ENV 27-FEB-2018 DEFINITION Uncultured Caudovirales phage clone 10F_4, partial genome. ACCESSION MF417980 VERSION MF417980.1 KEYWORDS ENV. SOURCE uncultured Caudovirales phage ORGANISM uncultured Caudovirales phage Viruses; dsDNA viruses, no RNA stage; Caudovirales; environmental samples. REFERENCE 1 (bases 1 to 6955) AUTHORS van Zyl,L.J., Abrahams,Y., Stander,E.A., Kirby,B.M., Clavaud,C., Farcet,C., Breton,L. and Trindade,M.I. TITLE Novel phages from South African skin metaviromes JOURNAL Unpublished REFERENCE 2 (bases 1 to 6955) AUTHORS van Zyl,L.J., Abrahams,Y., Stander,E.A., Kirby,B.M., Clavaud,C., Farcet,C., Breton,L. and Trindade,M.I. TITLE Direct Submission JOURNAL Submitted (29-JUN-2017) Biotechnology, University of the Western Cape, IMBM, C2, Life Science Building, Robert Sobukwe Rd, Cape Town, Western Cape 7550, South Africa FEATURES Location/Qualifiers source 1..6955 /organism="uncultured Caudovirales phage" /mol_type="genomic DNA" /isolation_source="healthy skin from forearm of human male" /db_xref="taxon:2100421" /clone="10F_4" /environmental_sample gene 278..1267 /locus_tag="10F4_2" CDS 278..1267 /locus_tag="10F4_2" /note="Similar to YP_950645.1 [Staphylococcus phage CNPH82] (BLASTp score : 486). Similar to PF03796.10 [DnaB_C] (hmmscan score : 246.0)." /codon_start=1 /transl_table=11 /product="putative DNA helicase" /protein_id="ASN72858.1" /translation="MQDQMNLLENYVIREAAQQTSEYQSMPNRDNFKYLIEQLQELDS MAIDKDNPTDNYLMEVMDNILSDKPKEFIKTGISSVDNRIMGFEAGQLNVLAGRPSTG KTSLALNIMWNIVLKGYPTTFFSLETGGNNIVERLVSSITNIPLHKVKQADGISDKET SDIMNAIDQIKKHNNLRIEDTAQITPQDIRERAMAQSDLPHVIFIDYLTLMQSDIPQR DRRLEVEKISRDLKIIAKETGSVIIALSQLSRGVESRQDKRPMMSDLREAGGIEQDAN MIFLLYRDDYYDKDLVDNDTGKSDIEFIIAKNKDGETGTVGLEFYKKSQRFYG" gene 1264..1476 /locus_tag="10F4_3" CDS 1264..1476 /locus_tag="10F4_3" /note="Similar to YP_008853767.1 [Staphylococcus phage phiRS7] (BLASTp score : 109)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72859.1" /translation="MKIEELQQLLGYLYRTTYKGDTDVQLCLLELGYAINRLLENGRL TPFNEYDENKEIIFNEYKGMNINGIN" gene 1463..1699 /locus_tag="10F4_4" CDS 1463..1699 /locus_tag="10F4_4" /note="putative phi-ETA orf24 like protein; Similar to YP_008853768.1 [Staphylococcus phage phiRS7] (BLASTp score : 155). Similar to PF11673.3 [DUF3269] (hmmscan score : 78.3)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72860.1" /translation="MGLIEGSKNKYYLYRDNDEKVVSVLPLSPNVNSVGNITGAYFSG GVKNMTDDELLNFKAVHNLYYEQELGSQLTLFDI" gene 1710..2111 /locus_tag="10F4_5" CDS 1710..2111 /locus_tag="10F4_5" /note="Similar to YP_008853769.1 [Staphylococcus phage phiRS7] (BLASTp score : 234). Similar to PF06356.6 [DUF1064] (hmmscan score : 142.6)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72861.1" /translation="MSKYNAKKVEYKGIVFDSKVECEYYKHLENRLHIDGFDEIEVQP RYELIPKIGKQRKSEYIADFALLSEGKLIEVIDVKGMATETAKLKAKFFRYLYPDVKL TWICKAPKYTGLEWITYEDLIKARRERKKAK" gene 2117..2320 /locus_tag="10F4_6" CDS 2117..2320 /locus_tag="10F4_6" /note="Similar to YP_008853770.1 [Staphylococcus phage phiRS7] (BLASTp score : 129)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72862.1" /translation="MEQTITLQIKVEVEQEVTVPVADNYDLEEISNSEANKVAEKYKN NPELIGFEDIKFRKVSDVQVKDY" gene 2334..2681 /locus_tag="10F4_7" CDS 2334..2681 /locus_tag="10F4_7" /note="putative phi PVL orf 50 family protein; Similar to YP_007237147.1 [Staphylococcus phage phi5967PVL] (BLASTp score : 85.9). Similar to PF07768.6 [PVL_ORF50] (hmmscan score : 76.2)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72863.1" /translation="MITVNAYGVEYDILGDNLKAVERNGISIQGLRNRILRGWSLHDA CHIPKRTDKAEYEMLKGVMAYEYDNTEAKEKYQDERLRKEKPHLFNVPQQHSRGKWCE YLMENDVFVKVAR" gene 2682..3404 /locus_tag="10F4_8" CDS 2682..3404 /locus_tag="10F4_8" /note="Similar to YP_008853772.1 [Staphylococcus phage phiRS7] (BLASTp score : 276). Similar to PF11753.3 [DUF3310] (hmmscan score : 72.1)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72864.1" /translation="MKIRDLDLDQYVIVYDMGKSEDSEGMTVVGRVEEIVFNDDNDNV ATINSLGNLYDITDDNDFELWSNYIESKTEHIAFDKENTKKAKKILSNDVQQRKRKDE DTALFKIEDLKVGYKVKVLDDNMIGEVSEISDNGKSAEIRVDDSVYRIINDNYDFTII EWNAVPQYPDDTVNHPSHYNYGDIEEINFIEQVTKHYNPNVAYHIGNAIKYLARSPHK NGKEDIDKARWYIERAFENWDK" gene 3404..3625 /locus_tag="10F4_9" CDS 3404..3625 /locus_tag="10F4_9" /note="Similar to YP_008853773.1 [Staphylococcus phage phiRS7] (BLASTp score : 114)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72865.1" /translation="MQLSNTIDIRYKYKTGGMNTKEMAQLLKYYGFRGYLKSVNQRSF TVAVLPEDKAHNRKVLNKLKGVMASETKS" gene 3609..3926 /locus_tag="10F4_10" CDS 3609..3926 /locus_tag="10F4_10" /note="Similar to YP_008853776.1 [Staphylococcus phage phiRS7] (BLASTp score : 70.5)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72866.1" /translation="MRRKVNNSETHEIKRYYKVPSEKVQEWSDTIENLTKQSDQFKAE RDTLIDDLSWYKAKVSRLEDESSKYIDLLEEFSQHIGNKPSSSTYKYFREKLDELGIK EGE" gene 3929..4189 /locus_tag="10F4_11" CDS 3929..4189 /locus_tag="10F4_11" /note="Predicted by MGA" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72867.1" /translation="MAFGIDLWKIVLESNCSEETERDIKIGLDDLYAKAKAFDEVKEF IIEKLKEEEEEKSRTLLSDYDYGLYQAYDNVDDIIQEYLERD" gene 4190..4429 /locus_tag="10F4_12" CDS 4190..4429 /locus_tag="10F4_12" /note="Predicted by MGA" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72868.1" /translation="MAYEYEGYIKNTIRTEAVFNHEKEKRQERVKELQEVYFKAEILN RIIGIVKNGRSLGSDTDSEIILRAIEHELKYTEDK" gene 4432..4641 /locus_tag="10F4_13" CDS 4432..4641 /locus_tag="10F4_13" /note="Similar to YP_007236648.1 [Staphylococcus phage StB20] (BLASTp score : 50.8)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72869.1" /translation="MSEIETKRIYDINRNKFHGVFQSAKPYTPLSVEGQSGQISFPVA VIEYENELRVVEPYQVRFIEEDKND" gene 4634..5164 /locus_tag="10F4_14" CDS 4634..5164 /locus_tag="10F4_14" /note="putative ORF019; Similar to YP_239842.1 [Staphylococcus phage 2638A] (BLASTp score : 235). Similar to PF00692.14 [dUTPase] (hmmscan score : 101.3)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72870.1" /translation="MTKLQIKLLSDNATKPKRADDESAGYDIYAAGTVILEPQEKALI ATDLAVNIPKGYVGLLTSRSGVSSKTHLVVETGKIDAGFQGHMKINVKNDMDISNVAP TKAVLNGERAYSLLDVSGERIGFSTNMNCYKINKGDRLAQLVIVPIWTPELVPVEEFE SETARGQNGFGSSGTR" gene 5201..5443 /locus_tag="10F4_15" CDS 5201..5443 /locus_tag="10F4_15" /note="Similar to YP_008853780.1 [Staphylococcus phage phiRS7] (BLASTp score : 60.8). Similar to PF07129.6 [DUF1381] (hmmscan score : 89.9)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72871.1" /translation="MTQYLIRTLTDSTGHTFTHVTKARENEKFTVVEAESKEEAKEKV TGGKSRLFDVKEIVLMSILSIWLFAGFIIFIFKFLL" gene 5756..5977 /locus_tag="10F4_16" CDS 5756..5977 /locus_tag="10F4_16" /note="Predicted by MGA" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72872.1" /translation="MNKFDSMIHKVVSPIFNFLEKYLTKFGNACIKMFKKLWEPFVNW ASEHRLITYSIIGVGYAFMIITAIYKYLL" gene 5991..6161 /locus_tag="10F4_17" CDS 5991..6161 /locus_tag="10F4_17" /note="Similar to YP_008853782.1 [Staphylococcus phage phiRS7] (BLASTp score : 80.5)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72873.1" /translation="MFWIIATIVLGCITILTLISNTIKNDQIAALKYTIVYMANDEDI EKAMQEWKRFKD" gene 6176..6403 /locus_tag="10F4_18" CDS 6176..6403 /locus_tag="10F4_18" /note="Similar to YP_008853783.1 [Staphylococcus phage phiRS7] (BLASTp score : 106)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72874.1" /translation="MDYKSCNKVESVRVDYLYFYEVYDEYYIEDLDNFAQSIDHHMNT NNYKVFEVDTLENERLIIDTTKIVNVRILYK" gene 6418..6813 /locus_tag="10F4_19" CDS 6418..6813 /locus_tag="10F4_19" /note="Similar to YP_008853784.1 [Staphylococcus phage phiRS7] (BLASTp score : 233). Similar to PB007043 [Pfam-B_7043] (hmmscan score : 158.9)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72875.1" /translation="MYTPTEVKQLITDYHWMRRLIDHQVYEYDSTSIGQYGIESAMPK AQGGTGDKVLVRVIKNDKDRRKTQELIEKVAFIDDYEHLITNDKNYHILQLLKQGESK NRIMVLMQISKDNLYNRIDAITEVYMNQQ" ORIGIN 1 aacctactgt atctgaaatt atggacagta aacccaagca gtcaaatgac atcataatac 61 cggaagaaga aactcacgag tacagaatga agcacgatgc agaatatgcc aaaaatcgtg 121 aagagttaaa aaataaatgg caacaaatga aaaaagaatg gatgagcgaa gatgactaat 181 ataaatgtgt taaacaccga agaagccatc gtctctaatc tcatgcgtaa tccagaatta 241 tttatacaat tcagacggca cagacaaagt tttcttcatg caggaccaaa tgaacttgct 301 agaaaattat gtaataagag aagctgcaca acaaacttct gaatatcaat caatgcctaa 361 tcgggataac tttaaatatc tcattgaaca actacaagaa ttagacagca tggcgataga 421 caaagataat cctactgata actatttgat ggaagtcatg gacaacatac taagcgataa 481 gccaaaagag tttatcaaaa ctggtataag ttcagtagat aacagaatca tgggatttga 541 ggctggacaa ctaaatgtgt tagctggtcg tccatcaaca ggtaaaactt cattagcgtt 601 aaacatcatg tggaacattg ttttgaaagg ttatcctact acattcttta gtttggaaac 661 tggtggtaat aacattgttg agcgtttggt atcaagcata acaaacatac cattacacaa 721 agttaagcaa gctgatggca taagtgataa agaaacaagc gacatcatga atgctataga 781 tcaaatcaaa aaacataata acttacgcat tgaagatact gcacagatta caccacaaga 841 tataagagaa agggcaatgg cccaatcaga tttaccacat gtaatattta tcgattacct 901 aacattgatg caatctgata taccacaacg tgatagacga ctagaagtag aaaaaatatc 961 acgtgacttg aaaattatcg ccaaagaaac aggcagtgta atcatagcgc tatcgcaatt 1021 aagtcgtggc gttgaatcaa gacaggacaa acgaccaatg atgagtgatt taagagaagc 1081 tggtggcatt gaacaagacg ctaatatgat tttcctatta tatcgtgatg attattacga 1141 taaagatcta gtggataacg acacaggcaa atctgatata gagtttatca ttgctaaaaa 1201 taaagacggc gaaacaggaa cggtcggatt agaattttat aaaaaatcac agaggtttta 1261 cggatgaaaa tagaagagtt acaacaatta cttggttatc tatacaggac gacatataag 1321 ggcgatacag acgttcaatt gtgtttgctt gaattaggct acgcaattaa tagattgctc 1381 gaaaacgggc gcctaacgcc ctttaatgaa tatgacgaaa ataaagaaat aatatttaat 1441 gaatataaag gtatgaatat aaatgggatt aattgaaggt agcaagaata aatattactt 1501 ataccgagat aacgatgaaa aagtagtatc tgtcttacca ttgtcaccaa atgtgaatag 1561 cgtcggaaat ataactggcg cttacttctc gggtggagtt aagaatatga ctgatgacga 1621 gttattaaat ttcaaagcag tacacaattt atattatgaa caagaacttg gaagccaatt 1681 aacactattc gacatctagg agtgacggaa tgagtaaata caacgctaag aaagttgagt 1741 acaaaggaat tgtgttcgac agcaaagttg aatgtgagta ttacaaacat ttagaaaatc 1801 gattgcatat agatggattc gatgaaatag aagtgcaacc aagatacgaa ctaataccta 1861 aaataggtaa gcagcgtaaa tcagaatata tcgcagactt tgcactgttg agtgaaggga 1921 agttaatcga agtaatagac gtgaaaggta tggccactga aacagctaag ttaaaagcta 1981 agttctttag atacttatat cccgatgtaa agctaacgtg gatatgtaaa gcacctaaat 2041 acacaggact agagtggatc acgtatgaag atttaattaa ggctagacga gaacgcaaga 2101 aagcgaagtg attccaatgg aacaaacaat cacactacaa atcaaagtgg aagttgaaca 2161 ggaagtcaca gtacctgtgg cagacaatta cgatttggaa gaaataagta acagtgaagc 2221 aaataaagta gctgagaaat ataaaaataa cccagagtta ataggatttg aagatattaa 2281 attcagaaaa gtatcagacg tacaagttaa ggattattag gaggatgaga aggatgataa 2341 cagttaatgc atacggtgtt gaatacgaca tattaggcga caacttaaaa gcagtagaga 2401 gaaatggtat tagcatacaa ggattgagaa acagaatcct aagaggttgg tcattgcatg 2461 atgcatgtca tattcctaaa cgtacagata aagcagaata cgaaatgctt aaaggtgtaa 2521 tggcttacga atacgataat acagaagcta aagaaaagta ccaagatgaa cgattgagaa 2581 aagagaaacc acatttgttt aatgtaccac agcaacacag taggggtaag tggtgcgagt 2641 atttgatgga aaatgacgtg tttgtaaagg tggctagata gatgaagatt agggatttag 2701 acttagatca atatgtaatt gtgtatgaca tgggtaagag tgaagacagt gaaggtatga 2761 cggttgtagg acgtgtggaa gaaattgtgt tcaatgatga taacgacaat gtagctacta 2821 tcaattcatt aggtaactta tacgatatca cagacgataa cgattttgag ttatggagta 2881 attatataga gagtaagacg gaacatattg catttgataa agagaatact aagaaagcta 2941 aaaaaatatt atccaacgac gtacaacaac gtaagcgtaa agatgaagat actgcattgt 3001 ttaagattga agatttaaaa gtcggttaca aagtcaaagt gttagatgac aacatgatag 3061 gtgaagtttc tgaaatatct gataatggca aatctgctga aataagagta gatgatagcg 3121 tgtacagaat tataaatgat aattatgatt tcacaataat agagtggaat gcagtgccac 3181 agtatccaga cgacacagtc aaccacccat cacattataa ctacggtgat atagaagaga 3241 taaatttcat tgaacaggta acgaaacact acaatccaaa cgtagcttat cacattggca 3301 atgctattaa ataccttgca cgttcaccgc ataagaatgg taaagaagat attgataaag 3361 ctagatggta tatcgagcgt gcctttgaga attgggataa gtaatgcagc tatcaaacac 3421 aatcgacata cgctacaaat ataaaactgg tgggatgaac accaaagaaa tggcacagtt 3481 gctaaagtat tacggatttc ggggatattt gaaatcggtt aatcaacgca gcttcacagt 3541 agcagtgtta ccagaggata aagcgcataa caggaaagtg ttaaataaat taaaaggagt 3601 gatggcgagt gagacgaaaa gttaacaatt cagaaacaca tgaaatcaaa agatattata 3661 aagtaccatc agaaaaagtg caagagtggt cagacactat agaaaactta actaaacaaa 3721 gcgaccaatt caaagcagaa cgagatacat tgatagacga tctatcttgg tataaagcaa 3781 aggttagtag gttggaagat gaaagttcta aatatattga cctactggaa gagttctccc 3841 aacacatcgg caacaaacca tcgagcagca cgtataagta ttttagagag aagttggatg 3901 agctagggat taaggagggt gaatagatat ggcatttgga atagaccttt ggaaaatagt 3961 gctagagtct aattgttcag aagaaacaga aagagatata aaaatcggat tagatgattt 4021 atatgctaaa gctaaggcgt ttgatgaagt aaaagaattt ataatcgaaa aattaaaaga 4081 ggaagaagaa gaaaaatcac gtacgttatt aagtgattat gattacggat tatatcaagc 4141 ttatgacaac gttgacgata ttatacaaga atatttggag cgtgattaga tggcgtatga 4201 gtatgagggc tatattaaaa atacaataag aactgaggca gtgtttaatc atgaaaaaga 4261 gaaaagacaa gagagagtaa aagaactaca agaagtctat ttcaaggcag aaattttaaa 4321 taggataata ggtattgtta aaaatggtcg ttctttaggt agtgatacag acagtgaaat 4381 catattacga gcaattgaac atgaattgaa atacacggag gacaagtaga catgagcgaa 4441 atagaaacaa aacgtattta cgacattaat agaaataaat ttcatggtgt gtttcaatcg 4501 gcaaaacctt atacaccttt gtctgttgaa ggtcaatcag gacaaatttc atttccagta 4561 gcagttatag aatatgaaaa tgaattgaga gttgttgaac cgtatcaagt tagatttatc 4621 gaggaggaca aaaatgacta aactacaaat caaactacta agtgacaacg caactaaacc 4681 taaacgagca gatgatgaat cagctggtta tgatatatac gcagcaggaa cagtgatact 4741 tgaaccacaa gagaaagcat taattgctac tgatctagca gtgaatattc ctaaaggcta 4801 tgtgggatta ctgacaagca gaagtggtgt aagtagtaag acacatcttg tagttgagac 4861 gggtaagatt gatgcagggt ttcaaggtca tatgaagatt aatgttaaga atgatatgga 4921 tatctcaaat gtggcaccaa cgaaagcagt tttaaatgga gaaagagcat attcattatt 4981 agatgtatca ggagaacgta tcggtttttc aacaaatatg aattgctaca aaatcaacaa 5041 aggagaccga ctagcacagt tagttatcgt accaatatgg acaccagagt tagtaccagt 5101 ggaggaattt gaaagtgaaa cagcaagagg gcaaaacggt ttcggttcaa gtggaacaag 5161 ataaagatat actcgaaaaa gttaaggaag tgttgaggaa atgacacagt atttaattag 5221 aacactaacc gattcaaccg gtcacacttt cactcatgta actaaagcac gtgagaatga 5281 aaagttcact gtggttgagg cagagagtaa ggaagaggcg aaagaaaagg taaccggtgg 5341 caaatcacga ttgtttgatg ttaaggagat agtccttatg tcaatactat ctatttggtt 5401 atttgcagga tttataatat ttatctttaa atttttatta tgagaaagga cagtgaccaa 5461 tgaaaatact taaaacacta ctaatcatag cagtatacga gttaagcaaa tacgttacga 5521 atgaaattat tacacgcaga caagcgaacg atgcggtgga tcagtacccg atggattatg 5581 aggtgagtga atgatgttca gtaaagcgca gctatgctat atcaatccat tgtactactt 5641 atacatttac ttgcgaataa gagatacagt tacgaatgta tggtatggaa tcaaaagtgt 5701 attagaaaag ttaccagttg aacatagaca taaattatta aaggatgtga agtagatgaa 5761 taaatttgat tcaatgattc ataaagtagt atctccaata ttcaacttct tagaaaaata 5821 tcttactaag tttgggaacg cttgtataaa aatgtttaaa aaactatggg aaccttttgt 5881 taattgggca agtgaacata ggttgataac ttattcaatt ataggtgtag gatatgcttt 5941 tatgattata acggcgatat ataaatactt attataaagg aggacgagta atgttctgga 6001 ttatagcaac aatcgtatta ggttgtatta caatactcac gcttatatct aatacaatta 6061 agaatgacca aatagcagca ttgaaatata caatcgttta tatggcaaac gatgaagata 6121 ttgaaaaagc tatgcaagag tggaagagat ttaaggatta actgggaggt agtgtatgga 6181 ttataaatca tgcaacaaag tagaaagtgt aagagtagat tatctttatt tttatgaagt 6241 gtatgacgaa tactatatag aagatttaga taacttcgca caatcgattg accatcatat 6301 gaacacaaat aactataaag tatttgaagt ggatacattg gagaatgaaa gattgattat 6361 agatacaact aagatagtta atgtgcgtat cttatataaa taacctggag gtaacatatg 6421 tacacaccaa ctgaagtgaa acaattaata accgattacc actggatgcg tagactcatt 6481 gaccatcaag tgtatgagta tgatagtacc tctataggac agtatggtat agagtctgct 6541 atgcctaaag ctcaaggtgg tactggagat aaggtgttag ttagagttat caagaacgat 6601 aaggatagac gtaagacaca ggagcttata gagaaggtag cattcattga tgactatgag 6661 catcttataa ccaacgataa gaactaccat atactacaac tacttaaaca gggtgagagt 6721 aagaatagaa taatggtact aatgcagata agtaaggaca acttatacaa taggatagat 6781 gctatcactg aagtatacat gaaccaacaa taaccagaca catcgtacga atcgtacaca 6841 tcgtacgcaa ttatgtactg tacataggta tggtatataa tatcgatata cgataagttc 6901 cagtgactta ttataaataa cagtactata ttatattgag gcacgttact tttgt //