LOCUS MF417993 10357 bp DNA linear ENV 27-FEB-2018 DEFINITION Uncultured Caudovirales phage clone 8F_10, partial genome. ACCESSION MF417993 VERSION MF417993.1 KEYWORDS ENV. SOURCE uncultured Caudovirales phage ORGANISM uncultured Caudovirales phage Viruses; dsDNA viruses, no RNA stage; Caudovirales; environmental samples. REFERENCE 1 (bases 1 to 10357) AUTHORS van Zyl,L.J., Abrahams,Y., Stander,E.A., Kirby,B.M., Clavaud,C., Farcet,C., Breton,L. and Trindade,M.I. TITLE Novel phages from South African skin metaviromes JOURNAL Unpublished REFERENCE 2 (bases 1 to 10357) AUTHORS van Zyl,L.J., Abrahams,Y., Stander,E.A., Kirby,B.M., Clavaud,C., Farcet,C., Breton,L. and Trindade,M.I. TITLE Direct Submission JOURNAL Submitted (29-JUN-2017) Biotechnology, University of the Western Cape, IMBM, C2, Life Science Building, Robert Sobukwe Rd, Cape Town, Western Cape 7550, South Africa FEATURES Location/Qualifiers source 1..10357 /organism="uncultured Caudovirales phage" /mol_type="genomic DNA" /isolation_source="healthy skin from forearm of human male" /db_xref="taxon:2100421" /clone="8F_10" /environmental_sample gene complement(487..939) /locus_tag="8F10_1" CDS complement(487..939) /locus_tag="8F10_1" /note="Similar to YP_003335768.1 [Escherichia phage D108] (BLASTp score : 65.5). Similar to PF08765.6 [Mor] (hmmscan score : 74.4)." /codon_start=1 /transl_table=11 /product="putative late gene transcriptional activator" /protein_id="ASN72993.1" /translation="MSNGELFDSGEVDKLEPDKVLAHMNDAVVLARWQGTLNEMASIA EHKLRQLLPDKPDEVPTIARAMVYAICETMGGAVVYIPRGDGLRRAIRDMEIFREWRE KNARPDALARKFDLSSQAVYDIIARQRALHRQQEPDLFGYEDAPKRLH" gene complement(936..1337) /locus_tag="8F10_2" CDS complement(936..1337) /locus_tag="8F10_2" /note="Similar to YP_950442.1 [Pseudomonas phage DMS3] (BLASTp score : 132). Similar to PF06252.7 [DUF1018] (hmmscan score : 92.2)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72994.1" /translation="MNRRNQQLSKIHIAKKDLGLDDETYRALLSRITGQSSAKDLSPL LVAKVLQEFERLGWQPKQGRAKPKPAADKAKLVSKIEAQLAEAGRPWEYGDGLAKRLY QVERLEWLDSKQLGGVVAALAKDAKRHGRRQ" gene complement(1334..2020) /locus_tag="8F10_3" CDS complement(1334..2020) /locus_tag="8F10_3" /note="Similar to YP_007392780.1 [Pseudomonas phage JBD24] (BLASTp score : 219). Similar to PF10979.3 [DUF2786] (hmmscan score : 61.6)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72995.1" /translation="MDRNKALDKIKKCLRLATSANPNEAAAAMRQAQALMKEHGIGQD DVSMADVMECTAVAGSKKTPAKWEAQLANTVSRAYTCKVLFASGIGRWNFIGEMAEVA GYTMAVLLRQVRQARRDFTLNKLQRCKLATKVRRADVFCEAWVHAVHKQVSAFAGATL SPAVEQYLAHHYPDLVQQKPRDRNDTSRRKAGVRAINDAMHGLLAAADVRLNHGMTTA APLALAQVAS" gene complement(2050..2463) /locus_tag="8F10_4" CDS complement(2050..2463) /locus_tag="8F10_4" /note="Predicted by MGA" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72996.1" /translation="MMQQADWDELKEQMSSPYGSMKLKCDGFEVDLVQVIQSGRKSWG TTVYVNGYIKGVWLSCDRKTGVPEHEETRRFYRKVSRSLYTAKEIEAWRKIWGKRETD KKAASKVFTYDWTWKSFRSLKKHLEANNTSIERLH" gene complement(2537..2764) /locus_tag="8F10_5" CDS complement(2537..2764) /locus_tag="8F10_5" /note="Predicted by MGA" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72997.1" /translation="MAKYQITVEDNETGVSVSVDNQPSGTASARVVATMMESARLIAR IELPAGFAHDEYSYESLMAYREKLHIKPTVH" gene complement(2804..3445) /locus_tag="8F10_6" CDS complement(2804..3445) /locus_tag="8F10_6" /note="putative gp05; Similar to YP_024678.1 [Burkholderia phage BcepMu] (BLASTp score : 241). Similar to PF11363.3 [DUF3164] (hmmscan score : 285.3)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72998.1" /translation="MSNIPEGFLKDAKGHLVPLDLVKPIDMARNDLVHELVGKAQVVS ATLAAFKADAFGDIKAFVDMSVEQYKTKVGGKKGNVTLMSFDGQYKVVLAAQDNIRFD ERLQAARALIDECLTEWTQDARSEVRAIVNEAFRADKQGEISTGRVLALRRMEITDAR WQRAMEAIGDAVQVVGSKSYIRVYQRVGESEQYVPIPLDIASASLNTTTHSVH" gene complement(3442..3918) /locus_tag="8F10_7" CDS complement(3442..3918) /locus_tag="8F10_7" /note="Predicted by MGA" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN72999.1" /translation="MAKWLEREVASGLMGLIALRLDGAPAADTVGATLDIWLVVLAKG REWDEHIDAERIRKAFQVLFATCERWPAPARLLRELPARMPAPALPKPSRTDKQKQTG NDALDGIVSGMRRSARISIGPKTDRQIDAARVGADAVFDHLQREALISKTMEQPSK" gene complement(3911..4408) /locus_tag="8F10_8" CDS complement(3911..4408) /locus_tag="8F10_8" /note="Similar to YP_002221436.1 [Burkholderia phage KS10] (BLASTp score : 71.6). Similar to PB006178 [Pfam-B_6178] (hmmscan score : 148.4)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN73000.1" /translation="MRTRCPNCGTTLSLDALIAHDGARDALGVAFKLSGPLGHALVRY LGLFRPETRELTMDRVAKLLNELLPDLQAQRIERNGQVFEAPPECWAWAIDQTLAARE TGRLVTPLKGHGWLYQVMTQYQPGSTGGALVLAPEAAQNLPVRRGNPSQTNAALAALE ARARG" gene complement(4408..4914) /locus_tag="8F10_9" CDS complement(4408..4914) /locus_tag="8F10_9" /note="Predicted by MGA" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN73001.1" /translation="MTLCIKDGQVEIRSPAKGMHRLYREGKCVGFAATLVRALAKAGE LRALFKLRESSGEKLAVEQITKEAVRSVVDESPVVASSPQVTAWVIDAYCWNGTKYAP VTVAAKFGEEGIKALLLGLETYHQYKPELTKPDDLMKWREKHPLGGKAVHAKYFRARE IPILPGVH" gene complement(5272..6048) /locus_tag="8F10_10" CDS complement(5272..6048) /locus_tag="8F10_10" /note="Similar to YP_950430.1 [Pseudomonas phage DMS3] (BLASTp score : 125). Similar to PB013709 [Pfam-B_13709] (hmmscan score : 139.8)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN73002.1" /translation="MAGRKAHLVVTGTKEPRQRIWECVRVLSLAFSINDIARRSGQMP DDINRYFKALVKAGIIKVVETPAGKVGRVYSLVKDEGVDHPRVNGRGERTYAHLATEN IWRTIRILNGNLTAESVAQAASSGEASLTVIKVRQYLNALTEAGYLEKTEFDVTTPEV YRLVPGKYTGPRPPEIRKLDSLQVYDPNLGKLVYTTTKGSVGADHTLVEPGIALLRTR DLLSEWLELAQSGKAVQPSADLLQRTQLELASPGESGGLQ" gene complement(6048..6770) /locus_tag="8F10_11" CDS complement(6048..6770) /locus_tag="8F10_11" /note="Similar to YP_009042185.1 [Pseudomonas phage JD024] (BLASTp score : 202). Similar to PF13401.1 [AAA_22] (hmmscan score : 63.7)." /codon_start=1 /transl_table=11 /product="putative DNA transposition protein" /protein_id="ASN73003.1" /translation="MSVTKIVPLTNVGLLSGAMSRAKNRPTNLPGLVAMYGPSGYGKS LAAAFAASQHRCYYTECRDTWSKKAFLSAILREMTVLPGRTLSEMVDQIAVQLSTSGR PLIVDDVQYLLDKAVANVLTDIYNASEGTIVLIGEERVPSSLQRLERLHNRVLEWVPA QAASFEDIKNLAKASYPEISFADDLLEELRKVTKGCLRRVVVNLHRIQSEASAAMLDS IGLDAWGKRDWFTGDAPSRRAV" gene complement(6833..8914) /locus_tag="8F10_12" CDS complement(6833..8914) /locus_tag="8F10_12" /note="Similar to YP_007392768.1 [Pseudomonas phage JBD24] (BLASTp score : 462). Similar to PF02316.11 [HTH_Tnp_Mu_1] (hmmscan score : 52.2)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN73004.1" /translation="MRNWLTAQELAGLPGLPGTVPGVRKVAIRENWASQKRNGTKAIE YAYSDLPAETQSAVLSHAISEAGEDTQPAPQAVNALVVNVEAKPALALDKVESKPAGP SAKLTDRQKAVMAARLAFVREIERMSAVVSQKTAIATLVKHAQDGHLSPYLQQRVLLA NDRKTAERGLSERTLKRWLAVWRAAGQDEASLAPMRQKASLEIPEWAPAFLRCYQRPT KPSVAASYTEFAATWTKTLPSIHAVNRFLKKLSPDALNIGRMGPQELKSLQAFNRRST VNIAPGEVYTADGHKFDAEVVNPITGKPYRPEITTVLDVATRRVVGISVGEAESAFGV LDALRDAVQKCMFAIFYVDNGSGFDNDTVREVVDRLGGTMKHSLPYNSQARGLSERGH QTIWVRAAKKLTSYIGADMDKHAGTKMHKISRKELKQHGKSRVLPEFGVFMAGVEHEI AHYNSTPHRGLEKIRDPETGVSRHMSPDEAWNTAIENGWEPMVAPTELVESLMRPQVV RTTRRGEISWNGSLYFMRDLENLHGQEIRVAYNVRDASQVWAYTLDGELIGPAMLDGN SSDYMPRTMLEAAREKRQQGQFNRAIGKIETLTGHKVEMIAPSVSPSANLPSEALEAA REYAAAIEQQKPKFEVPKNDVARYRLWEKLDERKAAGEELTADEAKWYERYPSHPDFV AMKEVFQQQAG" gene complement(8907..9275) /locus_tag="8F10_13" CDS complement(8907..9275) /locus_tag="8F10_13" /note="Similar to NP_938209.1 [Pseudomonas phage D3112] (BLASTp score : 96.3). Similar to PF13693.1 [HTH_35] (hmmscan score : 58.4)." /codon_start=1 /transl_table=11 /product="putative Ner-like protein" /protein_id="ASN73005.1" /translation="MDSHNIPLDLDSRWEWVKYQLRIRGTSIADLARDAGLNESAFRN AKRRAYPRVERDIANALGMLPGQIWPERWNADGTPLRLRPNRAESNVSPTGKDTGESS KGNNNPAERDDQQHRGNDDA" gene 9615..10166 /locus_tag="8F10_14" CDS 9615..10166 /locus_tag="8F10_14" /note="Similar to YP_007392308.1 [Pseudomonas phage JBD30] (BLASTp score : 194). Similar to PF00717.18 [Peptidase_S24] (hmmscan score : 30.3)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN73006.1" /translation="MPAFDSLAALAAARGVSLDWIATGQGEMCLADSEASEGALSAPD ADYAYIPLYDAYISQGHGAWNEGARVLAMLAFTRYSLRKKGLEPKQLAAVRVDGDSNE PELSEGDTVMVDLSRNHFQGEAFYVIRLNDLLFAKRLQREFDGSMSVISANSAYHPVR VPVERLDSLEIVGRVVWAGGWMI" ORIGIN 1 tgctcgttat tgtctttatc tgtgttcgta gtgttgttct tgtgtcattt gcatttttgt 61 ggtcgtctgt tgctcgtctc attgccttgg tgctgtggcg ctctgctttg tctgtgttgc 121 cgtctttgat ttatcaagca gacgtcggca tacgagtgtc tgcctgtata gtgggctggg 181 gttgacctgt aggtatggcc acctttgcag gcgtcctggg caacttgccg gcgatgaact 241 gcggcccgcg atatgacgtc ggttcgccat tcgctcgggc attcatcgat cattcgttgg 301 cgcgtcttct cgcagcgctc gtcaatgacg cgctgggcga attccgctga agaggtggcg 361 tgctcaagca tgttcgaatc tgccagatga atggacctgg ctaggttcgc ccgagcagca 421 agggcgctgt atttccgggg gctttaagca aaaagccccg ctaggcgggg cttgatgtag 481 gggcggtcag tgcaaccgct ttggagcatc ctcgtacccg aatagatcgg gctcttgctg 541 tcggtgtagg gcgcgctgac gggcaatgat gtcataaacc gcctggctgg atagatcgaa 601 tttgcgggcc aaggcatcag gccttgcgtt cttctccctc cactcacgga aaatttccat 661 gtcccgaatc gcccgacgca gcccatcacc ccgagggatg tagacgacag caccccccat 721 cgtttcgcaa atagcgtaaa ccatggcgcg ggcgattgtc ggcacctcgt ctggcttgtc 781 cggcagcaac tgcctcagct tgtgctcggc aatgctggcc atctcattca atgttccctg 841 ccagcgagcc agcactacag cgtcgttcat gtgtgccaac accttgtccg gctctagctt 901 gtcgacctcc ccactatcga ataactctcc gttgctcatt gtcgcctccc gtgtcgtttc 961 gcgtccttgg ccagagcggc gacgacgcca cccagttgct tggagtcaag ccactccagc 1021 cgctctacct ggtacaggcg ctttgccaaa ccgtcgccgt actcccatgg cctgccggcc 1081 tcagccagct gggcctcaat cttgctgacc agctttgcct tgtccgcagc cggcttgggt 1141 ttggcccggc cttgctttgg ctgccagccc agccgttcaa attcttgcag taccttcgcc 1201 accaggagcg ggctcaggtc tttggccgat gactgtccgg tgatccgact cagcaatgcg 1261 cggtaggttt cgtcatcaag gccaaggtct ttcttggcaa tgtggatctt gctgagctgc 1321 tggttgcgcc ggttcatgac gccacctgcg ccagtgccaa aggcgccgct gtggtcatgc 1381 catggttgag acgaacatca gccgccgcca gcaagccatg catggcatcg tttatggctc 1441 gcacgccggc cttgcggcgc gaggtgtcgt tacgatcccg aggcttctgt tgcaccaggt 1501 cggggtaatg gtgagccagg tactgctcca cggccggcga cagcgtggcg cccgcgaagg 1561 ccgacacctg cttatgcacg gcatgaaccc atgcctcgca gaacacatca gcacgccgca 1621 ccttggtagc cagcttgcac cgctggagct tgttcagcgt gaagtcacgg cgcgcctggc 1681 gcacctggcg aagcaacacg gccatggtgt agccggccac ctccgccatc tcaccaatga 1741 agttccagcg accgatcccg ctggcaaaca gcaccttgca ggtgtaggcc ctcgaaacgg 1801 tgttggccaa ctgcgcctcc cacttggccg gtgttttctt cgacccggcc actgcggtgc 1861 actccatcac atcggccatg ctcacatcgt cctggccgat gccgtgctct ttcatcagcg 1921 cctgcgcctg ccgcatcgcg gccgccgctt cgttggggtt ggcactggtg gccaacctca 1981 ggcatttctt gatcttgtcc agggccttgt tacggtccat aagattccct atctatcgcg 2041 tgtcgccgct cagtgcaggc gttcgatgct ggtgttgttg gcttccaggt gcttcttcag 2101 cgatctgaag ctcttccagg tccagtcgta ggtgaagacc ttgctggcag ctttcttatc 2161 ggtctctcgc ttgccccaga tcttccgcca agcctcaatc tccttggcgg tgtacaggct 2221 gcggctgacc tttcgataga agcggcgagt ttcttcgtgc tcaggtacgc cggtctttcg 2281 gtcgcagctg agccaaacgc ctttgatgta cccgttcaca tagaccgtag tgccccaaga 2341 ctttctaccc gactggatca cctgcaccag gtcgacctcg aaaccgtcgc acttcaactt 2401 cattgacccg taagggctgc tcatctgctc tttcagctca tcccaatcag cttgctgcat 2461 catgtcctcg gctgctcgtc agtacccggc caccacggcg ggcagaccaa ccccggctag 2521 gccggggcgg tttcgcttag tgaacggttg gtttgatgtg cagcttctcg cggtaagcca 2581 tgagggattc gtagctgtac tcatcgtgcg caaaccctgc gggcagctcg atacgcgcaa 2641 tgagcctggc gctttccatc atcgtggcga ccacccgcgc agaggcggtg ccgctaggct 2701 ggttgtcgac gcttacggat acgccggttt cgttgtcttc tacggtgatc tggtacttgg 2761 ccattcttac tactcacaga aattggtggt gatcgcaggc gaatcagtgc acggagtggg 2821 tggtggtgtt cagcgaagcg ctcgcgatgt cgagcgggat cggcacgtac tgctccgact 2881 caccgacccg ctgatacacg cggatgtagc ttttggagcc gacgacctgg acggcgtcac 2941 cgatggcctc cattgcgcgc tgccaacggg cgtccgtgat ttccatacgg cggagggcga 3001 ggactcggcc ggtgctgatt tcgccttgct tgtccgcccg gaacgcctcg ttgacgatgg 3061 cacgcacttc ggatcgggcg tcttgggtcc actcggtcag gcactcatca atcagggcgc 3121 gggcggcttg aaggcgctcg tcaaagcgga tgttgtcctg agcggccagc acaactttgt 3181 attggccatc gaacgacatc agcgtcacgt tgcctttctt gccgccgacc ttggtcttgt 3241 actgctcaac agacatgtca acgaaggctt tgatgtcgcc gaaagcatcg gccttgaatg 3301 cggcgagcgt ggccgatacc acctgcgcct tgcccaccag ctcatgcacc aggtcgttgc 3361 gggccatgtc gattggctta accaggtcca gcggaaccag gtggcctttg gcgtctttca 3421 gaaacccttc gggaatgttg ctcatttgct tggttgctcc atggtttttg aaatgagggc 3481 ttcacgctgc aggtgatcga acactgcatc agctcccact ctggcagcat caatctgcct 3541 gtctgttttc ggcccaatgc tgatacgagc actgcgcctc atgccgctaa cgatcccgtc 3601 aagggcatcg tttcccgtct gtttctgctt gtccgtgcgg cttggctttg gcagcgcagg 3661 ggcgggcatc cgtgctggca gttcccgcag gagcctggcc ggggccggcc agcgctcgca 3721 ggttgcaaac aggacctgga acgccttgcg gatacgttcg gcatcgatgt gctcatccca 3781 ctctcggcct ttcgccagca cgacaagcca gatgtcgagc gttgcaccta ccgtgtcggc 3841 ggcgggcgct ccgtctaggc gcagagcgat taggcccatg agcccggaag cgacctcacg 3901 ttcaagccac ttagccacga gcacgcgcct ccagcgccgc cagcgctgca ttggtctgac 3961 ttgggttacc acggcgtacc ggcaggtttt gcgccgcttc tggtgccaaa accagcgcac 4021 caccagttga gcctggctga tactgagtca tgacctggta cagccagccg tgccccttga 4081 gcggtgtcac cagtcgcccg gtttcacgcg cagccagcgt ttggtcgatg gcccacgccc 4141 agcactcagg cggagcttcg aacacctgcc cattgcgctc gatgcgctgg gcttggaggt 4201 ccggcagcag ttcgttgagc aacttggcca cacggtccat ggtcagctca cgggtttcag 4261 ggcggaacag cccgaggtaa cgcaccaggg catggccgag cgggccggac aacttgaagg 4321 cgacacccag tgcatcgcgg gcgccgtcat gtgcgattag cgcgtccagc gaaagcgtag 4381 tgccgcagtt agggcaacga gtgcgcatca gtgcaccccc ggcaggattg ggatttcccg 4441 cgcacggaag tacttggcat gcaccgcttt gcccccgagt ggatgctttt cgcgccactt 4501 catcaaatcg tcaggtttgg tgagttcggg cttgtactgg tgataggttt ccagacccaa 4561 caacagtgcc ttgatgcctt cttcaccaaa cttggccgct actgtgaccg gggcgtactt 4621 ggtcccattc cagcagtaag cgtcgatcac ccatgccgtt acctgcgggc tagacgccac 4681 cactggggat tcatccacta cggaacgaac ggcctctttg gtaatctgtt caactgccaa 4741 tttttcaccc gaactttcgc gaagcttaaa aagagcccgc agttcgccag ccttcgccag 4801 cgctcgaaca agggtcgcgg cgaaaccgac gcacttccct tcgcgataca gacgatgcat 4861 ccctttggct ggggagcgga tttctacctg accatcttta atgcacagag tcatttcggc 4921 ctccagagca attcgggttg ttcgggcact gctgacaaac tcgccagtgc cgcattgctt 4981 gcgggttatg ggtaggtgcc tggcgctgat aaaacccctg gcactgttca actgtcagcg 5041 agtcgccggt agcaacgcac tcaattcggc caagaacttc cattactcgg cgctcaatcc 5101 cgtcagtggt ttggctcgga tatttgttat cgagcaaaag gcttaccgcc gtacggctca 5161 tgtttatgcg gagcgccgca tgagtacgac ttgtggccgt tacctctttg gccaggagcc 5221 gcacgaacag cggtggctcg gctccccagc tcgataagtt cacggcccgt ttcattgcag 5281 gcctccagac tccccagggg aagccagctc cagttgggtg cgctgcaaaa gatcagccga 5341 cggctggacc gctttcccgc tttgagccaa ctccagccat tcgctaagca ggtcccgagt 5401 acgcagaagt gcaatccctg gctcaaccag agtgtgatca gcgccaactg aacccttcgt 5461 cgtcgtgtaa accagcttgc ccaagttagg gtcgtacacc tgaagagagt ccagtttccg 5521 gatctcgggc ggacgaggcc cggtgtactt gcctggaacg agccggtaga cctccggcgt 5581 cgtaacatcg aactcggttt tttccagata accggcctct gttagcgcat tgaggtactg 5641 gcgaaccttg ataacggtca gcgatgcctc acctgaagat gccgcctgtg caacactttc 5701 agcagtaagg ttgccattca aaattcgaat ggtgcgccag atattctcgg tggcgaggtg 5761 agcataagta cgctcgcccc gtccgttgac tcgcggatga tcaacccctt cgtccttcac 5821 aagcgagtaa acgcgcccaa cttttccggc tggggtctca actactttga taatccctgc 5881 ctttaccagc gccttaaagt agcgattgat atcgtcgggc atctgaccgc tgcggcgcgc 5941 aatatcattg atagagaaag cgaggctaag gactcgcaca cactcccaga tacgctggcg 6001 aggttctttg gttccagtca caaccaaatg cgctttacgt ccggccatca gacagccctc 6061 cggctaggag catcgcctgt gaaccagtcg cgcttccccc aagcatccag gccaattgaa 6121 tctaacatcg ccgcagacgc ctccgattga atgcgatgca gattcacgac tactcgccgc 6181 aggcaacctt ttgttacttt cctgagttct tccagaagat cgtccgcgaa ggagatttcc 6241 ggatagctcg ctttggcaag attcttgatg tcctcaaaag atgcggcctg cgctggcacc 6301 cactcaagta cacggttatg gaggcgttcc agtcgttgaa gtgacgatgg cacccgttct 6361 tcaccaataa ggacaatggt tccctcgctg gcgttataaa tatcggtcag gacgttggcc 6421 accgccttgt ccaagaggta ctgaacatca tctacgatca gcggccggcc agaggtggac 6481 agctggactg caatttgatc gaccatttca gaaagggtgc gacctggcag tacagtcatt 6541 tccctcaaga tcgcggagag aaaggctttt ttgctccagg tgtcgcggca ttcggtgtaa 6601 tagcaacgat gctggcttgc tgcgaatgcg gccgcaaggc tcttgccgta gccacttggg 6661 ccatacatag caacgagacc tggcagattc gtaggtcgat ttttggcccg gctcatagcg 6721 cccgaaagca agccgacatt tgtcaatgga acgattttgg ttacactcac tgtgcttctc 6781 ctgttgtctg gcgtgttggc gcacgccgac ctcttcctga taggggctct agtcagcccg 6841 cttgctgttg aaatacttcc ttcatcgcaa cgaagtctgg gtggcttgga taacgctcat 6901 accacttggc ttcgtctgcg gtgagttcct ctccagcggc cttccgctca tccaattttt 6961 cccaaagccg atacctagcg acgtcatttt tgggtacttc gaacttcggt ttctgctgct 7021 ctattgccgc tgcatactca cgcgctgcct ctagggcttc actcggcagg tttgcggacg 7081 gtgaaacgga cggagcgatc atttccactt tgtgcccggt taacgtctcg atcttgccga 7141 tagcgcggtt gaactgccct tgctgtcgct tctcgcgagc tgcttccaac atcgttcgtg 7201 gcatgtaatc ggagctgttg ccgtcgagca ttgccgggcc gatcagttcg ccatctaacg 7261 tgtacgccca gacctggcta gcatctctaa cgttgtaagc gacccgtatt tcttgtccgt 7321 gcaagttctc cagatccctc atgaaataga ggctcccgtt ccatgaaatc tcgcctcggc 7381 gggtagtcct caccacctgc ggccgcatca gagactcaac cagttctgtc ggagcgacca 7441 ttggttccca accattttca atggccgtat tccaagcttc atcggggctc atatgtcttg 7501 aaacccctgt ttcagggtcg cgaatctttt ccagccctct gtgtggggtg ctgttgtagt 7561 gagcaatctc atgctcaaca ccagccatga acacaccgaa ctctggcagc actctcgact 7621 tgccgtgttg cttcaactct ttccggctga tcttgtgcat ttttgtaccg gcatgtttat 7681 ccatgtcggc cccgatatac gacgtaagct tcttcgccgc gcggacccag atagtttggt 7741 gaccacgttc cgatagcccg cgagcctggc tgttgtatgg cagcgagtgc ttcattgtcc 7801 cgccaagtcg atctactact tctcgaacag tgtcgttatc gaagcctgag ccgttgtcca 7861 cgtagaaaat ggcgaacatg catttctgga ccgcatcacg aagcgcatcg agcacgccaa 7921 atgctgactc ggcttcacca acggatattc caactacacg acgggtagcc acatccagga 7981 cagttgtaat ttccggccgg tagggtttcc cggtaatcgg gttcactacc tcggcatcaa 8041 acttgtggcc gtccgccgtg tacacctcgc caggggctat gttcacggtt gagcgacggt 8101 taaacgcctg aagagacttc aactcttgcg gtcccatccg ccctatattc agggcgtcag 8161 gtgatagctt tttcagaaac cggttcacag cgtgaatgct tggcagtgtc ttggtccaag 8221 tcgcggcaaa ctcggtgtag ctggcggcca cgctaggctt tgtaggcctt tgatagcagc 8281 gaaggaaagc cggagcccac tctggtatct ccaggctcgc tttctgacgc ataggggcca 8341 gagaggcttc gtcttgtcct gcggcacgcc acacagccag ccagcgcttc agcgtgcgtt 8401 ccgacagacc tcgctcggct gttttccggt cgttcgcaag cagcactcgt tgctggaggt 8461 agggcgagag gtggccatcc tgagcatgct ttaccagcgt cgctatcgcg gttttctgcg 8521 agacgaccgc tgacatcctc tcgatctcgc gaacgaaagc cagccgcgct gccattacag 8581 ccttctgccg gtcagtcagc tttgccgaag ggcccgctgg cttactctcg accttgtcca 8641 gagcaagcgc tggcttagcc tcgacgttga ccacaagtgc attaacagcc tggggagccg 8701 gctgagtgtc ttcgccagct tcggatatgg catgcgacag cacagcggat tgagtttctg 8761 ctgggaggtc ggaataggcg tattcgatgg ctttcgtgcc gttgcgcttc tgagaggccc 8821 agttttcacg gatggccact ttgcgcactc ccggaacggt gcctggtagg cctggcagtc 8881 cagccagctc ctgagctgtt aaccaattac gcatcgtcgt ttcctcgatg ctgctggtca 8941 tcccgttcag ctggattatt gttacctttc gaactttctc cggtatcctt accggttggc 9001 gagacattgc tctcggcgcg atttgggcgg agtcgcagag gggtgccatc ggcattccag 9061 cgctcaggcc agatctggcc aggcagcatg cccaatgcat ttgcgatatc acgctcaact 9121 cgcgggtaag cacgacgttt ggcgttacgg aaggcgcttt cgttaagtcc cgcatcgcga 9181 gcgaggtcgg cgattgaggt gcctcggatg cgcagctggt actttaccca ttcccagcgg 9241 ctatccagat ccagcggaat attgtgactg tccatgatgg ggtcattccc gaattagggg 9301 ttattgcccg gcccgctaac ggccggtttc gaacaatata accccgtata cgggaagcag 9361 taaaccggat ttgggagttt ttattgcgta tttcggatgg gcggcgtccg atcaacggca 9421 aaccccaatg aaatcagtga cttagacgag aatagaaacc gttgagcgat gaccctaaaa 9481 aagtttcttt agcggatacg ggaatagaaa cccggattgc ggctgtcgcg aacctttacg 9541 agacccgaaa acaggctgcg ctcactgccg gagtagccat gtcctcgctc tcacgttgga 9601 tcgcaggcga aggaatgccc gcatttgact cgctggccgc tctagcggca gcgcgtggcg 9661 tttctttgga ttggatcgct acaggtcagg gggagatgtg cctagctgat tctgaggcct 9721 ctgagggggc tctaagcgcg cctgacgcgg attacgctta catcccgctc tatgatgcct 9781 acatcagcca aggccatggc gcctggaacg agggcgctcg ggtattagca atgcttgcct 9841 ttacccgcta tagccttagg aaaaaagggc tggagcccaa gcagcttgcc gctgtcaggg 9901 ttgatgggga ttcgaatgag cctgagctga gcgagggcga taccgtcatg gtcgatttga 9961 gtcgcaacca tttccagggc gaggctttct atgtcattcg tctgaatgac ttgctctttg 10021 cgaagcgtct acaacgggag tttgacggca gcatgtcggt gattagcgcc aactcggcct 10081 accaccctgt gcgcgtccct gtagagcgcc tggatagcct ggagatcgtt gggcgtgttg 10141 tgtgggcggg cggctggatg atttgattcc gagggccaaa gagggcgcca aaatcgaccg 10201 attggcgcct tttcctcggt ttctatctta gcgcctcttt ggccctgctt tggcaccgtt 10261 tcttttcttc tagcccaatg aatccgggct tttccacttt cttcccgctt cttcccgcct 10321 tttttctctt agtgccaaac agaacacctc ctcacag //