LOCUS MF417994 11432 bp DNA linear ENV 27-FEB-2018 DEFINITION Uncultured Caudovirales phage clone 8F_3, partial genome. ACCESSION MF417994 VERSION MF417994.1 KEYWORDS ENV. SOURCE uncultured Caudovirales phage ORGANISM uncultured Caudovirales phage Viruses; dsDNA viruses, no RNA stage; Caudovirales; environmental samples. REFERENCE 1 (bases 1 to 11432) AUTHORS van Zyl,L.J., Abrahams,Y., Stander,E.A., Kirby,B.M., Clavaud,C., Farcet,C., Breton,L. and Trindade,M.I. TITLE Novel phages from South African skin metaviromes JOURNAL Unpublished REFERENCE 2 (bases 1 to 11432) AUTHORS van Zyl,L.J., Abrahams,Y., Stander,E.A., Kirby,B.M., Clavaud,C., Farcet,C., Breton,L. and Trindade,M.I. TITLE Direct Submission JOURNAL Submitted (29-JUN-2017) Biotechnology, University of the Western Cape, IMBM, C2, Life Science Building, Robert Sobukwe Rd, Cape Town, Western Cape 7550, South Africa FEATURES Location/Qualifiers source 1..11432 /organism="uncultured Caudovirales phage" /mol_type="genomic DNA" /isolation_source="healthy skin from forearm of human male" /db_xref="taxon:2100421" /clone="8F_3" /environmental_sample gene complement(<1..1349) /locus_tag="8F3_1" CDS complement(<1..1349) /locus_tag="8F3_1" /note="Predicted by MGA" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN73007.1" /translation="MAQIPLGNFAQARALPEAPQNRVITVDNREQAQAAQQAASSVQN AALGVLDTINKEDQALARVKADNALIDRESQITTIATDLDEQMRTGKLSYDKAPEAYE SAVSKLDPLETPGLDEAQQGELGNVLKRMQVRGFGKVQAAVAKGRIQAAQSDLVSRMD MLGKDAAMPGANVDQINARMDAEDIDVAGRLAFGETWASKKQEFKDGNWTTHATQRVI ESRESIGSLQKLEHDLTAEDGFYAKKLDPEKRNQLLNTVSGRIFQVKEHQQRQAEMRE MKAERILTQMDRQAATGVPPTPADQQRWKSALSGTSAAGEFNTRMQEMTQVQTLLRQP PAVAQQFIDRQRQQMQSNGASVAEQANLDRLQRAVDSNTKMMRETPLVFNAMRTGADV APLDVSGIASPEGQQKLGDQIAERFDVVNSVRKAYGPDVNRNPWKPEEQVMLSSLIK" gene complement(1353..1865) /locus_tag="8F3_2" CDS complement(1353..1865) /locus_tag="8F3_2" /note="Similar to PB017371 [Pfam-B_17371] (hmmscan score : 57.2)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN73008.1" /translation="MAVGAISAAGYAYAALAAATVYSVYSTQQSGKQAQLNADAQSDQ AQSDADAAASAAVVQADRIRRLARNQSSEANAALAASGVETGAGTAININEEIIGNAE EDAALTIFNGKNQKARGYADASNYSMAGNQARSAANSESVGTVLSAGAKATSMWKASA EAKIPKVGGR" gene complement(1867..2310) /locus_tag="8F3_3" CDS complement(1867..2310) /locus_tag="8F3_3" /note="Similar to YP_007010672.1 [Pectobacterium phage PP1] (BLASTp score : 53.5)." /codon_start=1 /transl_table=11 /product="putative acyl-CoA N-acyltransferase domain-containing protein" /protein_id="ASN73009.1" /translation="MIRPAKHSDVPRLIELGTLLHSTTSYSTMNFCPVKSAAFLHELI NGQGVVFVAEVRGEVVGGMAGGVIDQWFSNDLIAYDYSLFVEPSKRNGVIAVRLIQTF KEWATIKGAKQIYMGIGTGVSVEGTTRLYESQGLRNIGPLLMMEI" gene complement(2320..4482) /locus_tag="8F3_4" CDS complement(2320..4482) /locus_tag="8F3_4" /note="Similar to YP_007517711.1 [Pelagibacter phage HTVC010P] (BLASTp score : 194)." /codon_start=1 /transl_table=11 /product="putative tail tubular protein B" /protein_id="ASN73010.1" /translation="MPRMTLNQTNFTAGEVSPRMLGRVDIARYQNGAEIIENAWPVIH GGCVRRDGTLLCSPAKFPDKNCRLIPYVFNASQAYMVEFGDLYVRIHFANGSYSGIEL ASPYAHTTLDRIDYVQGADTMFIFCNTVPVYRLRRITNTEWSLAPAPFVTKPFDEKGI DFLTAITIDNPAVGTGRTVTASESAFLASDVGREIWSGGGVAKVTAVTSATVATVEVT NAFSATSRPTWSLKGSPQTSNTLSAFTPVGSVVTMTLATAGWRASDVGKFVKINGGLL EITVFTSTTVVSGVIRSAPTSATASPANAWSLEASVWNDIDGYPGAGTLYEQRLALGG SPNFPQTIWESRTGEYLNFELGTKDDDAISYNLSSDQINPILHIGQINALIPLTYGGE FTVSGGVEKAITPTNIRAKNPSVYGCNRVRPVRIGNELYFIQRAGRKLRAMAYKYDSD TFGSPDMSVLSEHATKSGIIDMAYQQEPESILFMVRADGVLATMTVDRDQDVIGWARQ ITDGAFESAASIPTDSGDQVWVVVRRNIGGANVRYIERVTNGVRVDSGVNGTSVGGAT VWGGLAHLEGKMVDIVADGVVMQQQVVTGGQVTIPRKAFATSIGLNFKTRIKTLTPEA QGNTGSSQGNSMRIGEVTLRFLDTIGCKVQGKGLAQTISFRNLGENVLDQPPANFIGV KRIENLGWERGEASLEIMQDQPLPFHLLNVIKKITIND" gene complement(4482..5069) /locus_tag="8F3_5" CDS complement(4482..5069) /locus_tag="8F3_5" /note="Similar to YP_007517710.1 [Pelagibacter phage HTVC010P] (BLASTp score : 117). Similar to PB003364 [Pfam-B_3364] (hmmscan score : 182.4)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN73011.1" /translation="MAMATGVSICSNALLMLGSQTINDFEDQQNLDRAKLCANLYPTV RDDMLRSHPWNCCIKRAVLAPDAVAPLFGYAHAFELPSDFSRVLEVGSHGCQIDYLVE GRTIQANTAVLELRYVFRNEVENTWDAHLVKLVTLAMAAAMAYPVTQSAAMQQTMEQK LETSLRRARAVDGQEDPPQTLGDERLLAARFGGGW" gene complement(5143..5667) /locus_tag="8F3_6" CDS complement(5143..5667) /locus_tag="8F3_6" /note="Predicted by MGA" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN73012.1" /translation="MAVVTTKSTAVTNSDALPQTLSPQRIDGGRLRERVGFVEATATD SIGSVYRLMRINSVDRVSRLLLSCDAISTAAGNIGLYDVTAVNAGAVVDADFFASAQD LSAALANTDVTHEADAADAGAGFGLADIEKPLWQALGLAADPGKQYDVAITLTAAATG AGTISLKLQYIDGN" gene complement(5711..6574) /locus_tag="8F3_7" CDS complement(5711..6574) /locus_tag="8F3_7" /note="Similar to YP_007517707.1 [Pelagibacter phage HTVC010P] (BLASTp score : 162). Similar to PB015091 [Pfam-B_15091] (hmmscan score : 277.5)." /codon_start=1 /transl_table=11 /product="putative major capsid protein" /protein_id="ASN73013.1" /translation="MSFQITEAFVQQYGANFRHLAQQMTSRLEGHVTIEPNIVGMSKS INRLGQRTAKRRTTRHGDTPINDQPHSTRFVDLFDWEDGDMIDDQDKIRMLVDPTSDY VKAMVSSLNRAKDDVIIGSFGGFSRSTTGNILLPTKQKIAVGGTGLTKAKIIQARKLF RQNEADNHNGEELCITYSAQAAADILADPTLTSADYMAGKFLESGDVEGKWMGFTWIP SERTPLSGGVRLLYAWAKSGVTLGKGADITTKVGEDPGKGFNVRIYAKMSIGSVRVEE EKVVEIAAAEA" gene complement(6771..7622) /locus_tag="8F3_8" CDS complement(6771..7622) /locus_tag="8F3_8" /note="Similar to PB003449 [Pfam-B_3449] (hmmscan score : 103.7)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN73014.1" /translation="MNMFIHGRLGRFFMAETTEGEPGATTTTAATTTTATGTVLDSGN TGNDYIPEKYRTNKEDGSLDLEASSRKVAEAYKHLETRMGSGDVPPKTAEEYAVKLEG VEGFNWDEFKTDPDTQSFLKGAHAKGLTNDQVQYVIGEYMKAAPGLIEGGGQLTQQDC TAALKAVWTDEQAMKQNVRASFRAAEAFASKPGEPGNFDTLQAKYGNDPDFIAFTANI GKELKEDTAINGGAQVSEADFDVKDSELRSQLQALPAHDPKRKGIQAELDAMYERRYN KSSSRLS" gene complement(7625..7870) /locus_tag="8F3_9" CDS complement(7625..7870) /locus_tag="8F3_9" /note="Predicted by MGA" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN73015.1" /translation="MTMNPEQIDAMFKRVFEEHHEGAMCLELLIQRFSRNACTIGGID AILTTYKQAGAREVLDHVVTRINRANGVQADPNEQEE" gene complement(7867..9462) /locus_tag="8F3_10" CDS complement(7867..9462) /locus_tag="8F3_10" /note="Similar to YP_007517703.1 [Pelagibacter phage HTVC010P] (BLASTp score : 263). Similar to PF12236.3 [Head-tail_con] (hmmscan score : 441.0)." /codon_start=1 /transl_table=11 /product="putative head-tail connector protein" /protein_id="ASN73016.1" /translation="MNADQIAKTLGTLKSLRLPHETVWRDCFDHSYPIRGSGFCTEQI SAIEAQMRKARMIDGTTTDAARILSSGIMSGLTPANSLWFGMDVGQETEEERRWLDDS ADILWQNIHASNFDAAAFEGLIDVVCAGWFALYIDQDMEKGGFTFDLWPIASVYASAS KAGGKIDTVYREYKLTAEQAVNEFGEENVSENTRKLAKDKPQEMVRFVHAIYPRTTHM VNAKLAKNMPIASCKVEVEAKKLVSESGYHEMPVVVPRWMMIPDSVYAVGPVFDALPD ARTLNELCRMDLAAGDLAIAGMWIAEDDGVLNPRTVKVGPRKIIVANSVDSMKPLQSG SNFQYAETKIARLQGSIRKILMADQLQAQDGPAMTATEVHVRVNLIRQLLGPVYGRLQ TEYLQPMIERCFGIAYRAGVLGAAPESLAGRNFTVRYLSPLARSQKLEEVTAIDTFVA GALAIAATDPTVMDNIDMDEAQRFKGEALGVPGSIIRSTADRDKIRADRAQAQQAAME QEQQQAMMQQAGEAALKQQGAAA" gene complement(9459..9662) /locus_tag="8F3_11" CDS complement(9459..9662) /locus_tag="8F3_11" /note="Predicted by MGA" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN73017.1" /translation="MGSKPKAPKTVATPDPAVEAQKAADLAAQKANEETATRKKRKQE SSLLSTGGAAGSVLDQGKRTLGS" gene complement(9667..10113) /locus_tag="8F3_12" CDS complement(9667..10113) /locus_tag="8F3_12" /note="Predicted by MGA" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN73018.1" /translation="MPAPDLTPQTPGEPLATITPLAGAISDASTGNPNASELNTANAQ ASAQPLFAAKHNGGGRWIVIDDKAPLVDGKVVKIGDFVGASKEEAETEAERLNAGGDP LVLDPQRTSEPAPKAQASTGNHDATTLKQAVLTNDGWLCPEPKAKD" gene complement(10142..10519) /locus_tag="8F3_13" CDS complement(10142..10519) /locus_tag="8F3_13" /note="Similar to YP_008126176.1 [Vibrio phage pYD38-A] (BLASTp score : 181). Similar to PF13876.1 [Phage_gp49_66] (hmmscan score : 123.1)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN73019.1" /translation="MTSVTDQAIEQEIQAKGLTAPRVTPADLKANIVGEYFFTAADGV QAAFNSQDELTRLTGAHGELALLTFCVLVLRNGFTVTGESACASPENFDAEIGRKIAR DNAVARVWPLMGYALKQHLNDEA" gene complement(10592..11038) /locus_tag="8F3_14" CDS complement(10592..11038) /locus_tag="8F3_14" /note="Similar to PF03245.8 [Phage_lysis] (hmmscan score : 65.5)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN73020.1" /translation="MRIILGICAALAIGLLLALWRLDHVSARLTTSTERVGVLEGQAA AYEQALTVRDSIDSQYQEAIHNAEQSKPQLVTDLNAGVKRVYVRAACVPANPQPTGSA DAAAPELAADARQDYADLVAAHAKVTAQVIGLQDFITRACSRSPSK" gene complement(11035..11283) /locus_tag="8F3_15" CDS complement(11035..11283) /locus_tag="8F3_15" /note="Similar to YP_002308525.1 [Bacteriophage APSE-2] (BLASTp score : 97.4). Similar to PF00959.14 [Phage_lysozyme] (hmmscan score : 53.4)." /codon_start=1 /transl_table=11 /product="putative lysozyme" /protein_id="ASN73021.1" /translation="MAGVDRNVTKPQPETRRAALASFVYNVGEPQFKASTLLRKLNAG DVRGACAELSRWVYAKGTKLKGLVNRRAAERELCEVGL" ORIGIN 1 tgcttgatca gcgaggacaa catgacctgt tcttctggct tccacgggtt tctgttgacg 61 tcggggccgt atgccttgcg cacagagttg accacgtcga agcgctcggc gatctggtcg 121 cccagcttct gctggccctc tggactggcg atccctgaaa cgtccaacgg cgccacatcg 181 gcgccagttc gcattgcgtt aaacaccagc ggggtttccc gcatcatctt ggtgttgctg 241 tccactgccc tttgcaagcg atccaaattg gcttgctcgg ccacgctggc gccatttgat 301 tgcatctgct ggcgttgacg gtcgatgaac tgctgggcga ccgctggcgg ctgtctcagc 361 agggtttgca cctgcgtcat ttcctgcatg cgggtattga actcgccggc ggcggacgtg 421 ccggacagtg ccgacttcca acgctgctga tctgctggtg tcggaggcac acccgttgcg 481 gcctggcggt ccatctgcgt caggatgcgt tcagccttca tttcccgcat ttcggcctgg 541 cgctgctggt gttccttcac ctgaaagatt cgaccgctca cagtgttgag caactggttg 601 cgcttctccg ggtccagctt cttggcgtag aaaccatcct cggcggtgag gtcatgctcc 661 agcttttgca ggctgccgat gctttcgcgg gattcgatca cccgctgggt ggcgtgcgtg 721 gtccagttac cgtccttgaa ttcctgcttt ttgctggccc aggtctcacc gaatgccagg 781 cgcccggcca cgtcgatatc ctcggcgtcc atgcgggcgt tgatctggtc gacgttggca 841 ccaggcattg cagcgtcttt gcccagcata tccatgcgcg ataccaggtc gctctgtgct 901 gcctgaatgc ggcccttggc tactgctgcc tgaaccttgc cgaaaccacg cacctgcatc 961 cgcttaagaa cgttgcccag ctcaccctgc tgcgcttcgt caagaccagg agtttccagc 1021 gggtcgagct tagacactgc tgattcgtat gcttccggcg ccttgtcgta gctcagcttg 1081 ccggtgcgca tctgttcgtc aagatcagtg gcaatggtcg tgatttgaga ttcacggtcg 1141 atcagcgcgt tatcagcttt gacccttgcc aacgcctgat cttccttgtt gatggtgtcc 1201 agcacgccca gcgcggcgtt ctgcacggag cttgctgcct gttgcgctgc ctgggcctgc 1261 tcacggttat ccacggtgat gacgcggttt tgcggtgcct cgggcaatgc tcgggcctgt 1321 gcgaagttac ccagcggaat ctgtgccatc agttagcgcc cccctacttt aggaatcttt 1381 gcttccgccg acgccttcca catgctggtt gcttttgccc cagcagataa aacagttcct 1441 acggattccg agtttgcagc actgcgtgcc tggttgcccg ccatgctgta attgctcgcg 1501 tcggcatagc cacgtgcctt ctggtttttg ccgttaaaga tggtcaacgc cgcgtcttcc 1561 tcggcattgc caatgatttc ctcgttgatg ttgatcgcgg tgccggcccc cgtctcgaca 1621 ccagatgcag ccagggcagc attggcctcg ctggactggt tgcgcgccag acggcggata 1681 cgatcggcct gcactacggc ggcactggct gctgcgtctg catcgctttg cgcctgatcg 1741 gactgggcat cagcattgag ctgcgcttgt ttgccagatt gctgggtcga ataaacggaa 1801 tacacagtcg ctgcggccaa tgccgcatat gcgtaccctg ctgcgcttat tgctcctacg 1861 gccatgtcag atctccatca tcaaaagcgg gccgatgttg cgcaggccct gggactcata 1921 aagccgggtg gttccctcga cgctgacgcc ggtaccgata cccatgtaaa tctgtttcgc 1981 gcccttgatc gtggcccact ccttgaacgt ctggatcagg cgaacggcga tcacgccatt 2041 gcgcttggac ggctcaacaa acaaggaata gtcataggcg atcaggtcat tgctgaacca 2101 ctgatcaatc acgccgccgg ccatgccgcc gacaacttcg ccgcgcacct cagccacaaa 2161 gacgacgccc tgcccattga tcagctcatg caggaaggct gcagacttga ccgggcaaaa 2221 gttcatggtt gagtagctgg tggtggaatg cagcaacgtg cccagctcaa tcaagcgggg 2281 cacgtcggaa tgtttagcgg gcctgatcat tggggcgcct caatcgttga tggtgatttt 2341 tttaatgacg ttgagcagat ggaacggcaa aggctggtcc tgcatgattt ccagtgacgc 2401 ctcaccgcgc tcccacccca ggttctctat gcgcttgacg ccaatgaagt tggccggcgg 2461 ctggtcgagc acgttctcgc ccaggttccg aaagctgatt gtctgggcca gacctttgcc 2521 ttgaaccttg cagccgatgg tgtcgaggaa tcgcagcgtg acctcgccaa tgcgcatgct 2581 gttgccctgt gagctgccgg tgttgccctg cgcttctggg gtcaacgtct tgattctggt 2641 cttgaagttc aggccaatcg aggtggcgaa cgccttgcgc gggatggtta cctgcccgcc 2701 cgtgacgacc tgctgctgca tcacaacgcc atccgcaacg atatcgacca tcttcccttc 2761 aaggtgcgcc aggccacccc acacagtggc accgcccacg ctggtgccat ttacgccgga 2821 gtccacccgc acaccgttgg taacccgctc gatgtagcgc acgttggcgc cgccgatgtt 2881 gcggcgaaca accacccaca cctggtcgcc gctatctgta gggatggatg cggctgattc 2941 aaaggcgccg tcggtaatct ggcgagccca gccaatcacg tcctggtcac ggtccacggt 3001 catggtggcc agaacgccgt cggcccgaac catgaacaaa atggattcag gctcctgctg 3061 gtaggccatg tcgatgatgc cggacttggt cgcgtgctca gacagcacgg acatgtcagg 3121 ggagccgaac gtgtcggagt cgtacttgta ggccatggcg cgcagcttgc ggcctgcacg 3181 ctggatgaaa tacaactcgt tgccaatgcg cacggggcgc acgcggttgc agccgtagac 3241 ggatgggttc ttggcgcgga tgttggtcgg ggttatggcc ttctcaacgc cgccgcttac 3301 cgtgaactcg ccgccatagg taagcgggat cagggcattg atctgcccaa tgtgcaggat 3361 ggggttgatc tggtcagagg acaggttgta ggagattgca tcgtcgtcct tggtgcccag 3421 ttcgaagttc aggtactccc cggtacgcga ctcccaaatg gtttggggga agttcggcga 3481 gccgccaagg gccaagcgct gctcgtagag cgtgccggcg cctgggtagc cgtctatatc 3541 gttccagaca gaagcctcaa gcgaccaggc gttggcaggc gatgctgtgg ccgaggtcgg 3601 cgccgagcga atgacgccgg aaaccactgt ggtgctggtg aatacggtta tctccagcag 3661 gccaccattg atcttgacga acttgcccac gtcactggcg cgccagcctg ccgtcgcaag 3721 tgtcatggtt acaacgctgc ctactggggt gaaagcgctc agcgtgttgg aggtttgcgg 3781 cgatcccttc aaggaccagg taggacgact ggtggcgctg aatgcgttgg tcacctcgac 3841 ggtggccacg gttgcgctgg taactgccgt gaccttcgca acaccaccgc ccgaccagat 3901 ttcacgacca acatcagagg ccaggaacgc cgattcagat gccgttaccg tgcgcccggt 3961 gcccactgct gggttgtcaa tggtgatggc cgtcaggaaa tcgataccct tttcatcgaa 4021 cggcttggtc acgaaaggcg ccggggccag gctccactcg gtgttagtga tgcggcgcag 4081 gcggtagacg ggaaccgtgt tgcagaagat gaacatggtg tccgcgccct ggacgtagtc 4141 gatgcggtcc agggtggtgt gcgcgtaggg gctggccagt tcgatcccgc tgtaggaacc 4201 gttagcaaag tggattctga cgtacaggtc gccgaactcc accatgtacg cctgggaggc 4261 gttgaacacg taagggataa ggcggcagtt cttatcgggg aacttggcgg gcgaacacaa 4321 caaagtccca tcacggcgaa cacaaccgcc gtggatgacg ggccaagcgt tctcgatgat 4381 ttcagcgccg ttctggtacc gggcaatatc gacacggccc agcattcgcg gggagacctc 4441 gccggcggtg aagttggttt ggttcagcgt catgcgaggc attaccagcc acctccaaaa 4501 cgagcagcca gcaagcgttc atcgcccaaa gtctgcggtg gatcttcctg gccatcgacc 4561 gcacgggcac ggcgcagcga ggtttccagc ttctgttcca tggtctgctg catggctgcg 4621 gattgcgtga ccgggtatgc catggccgct gccatagcca gggttaccag cttcaccagg 4681 tgcgcgtccc aagtgttttc tacctcgttg cggaacacat agcgcagctc cagcacggcg 4741 gtgttcgctt ggatggtgcg cccctcaacc aggtaatcaa tctggcaacc gtgggagcca 4801 acctccaaga cgcgggagaa gtccgacggc aactcaaagg catgggcata gccgaacaga 4861 ggcgccacgg cgtcaggtgc caacacggcg cgcttgatgc agcagttcca ggggtgcgag 4921 cgcaacatgt cgtcgcgcac tgtgggatac aggttggcgc acagcttggc ccggtccagg 4981 ttctgctgat cttcaaagtc attgatggtc tgcgagccca gcatcagcag cgcgttagag 5041 caaatcgaaa cacccgtggc cattgccatt accaacctcc agataaaaag accggggcac 5101 agggccccgg taaatgtttt gccttccttg gcggcccgtg aatcagttgc cgtcgatgta 5161 ttgcagcttc aagctgatag tgccggcacc ggttgcagcg gcggtcaggg tgatagccac 5221 gtcgtattgc ttgcctgggt cagcagcgag gcccagcgcc tgccacagtg gtttttcaat 5281 gtcggccagg ccgaagcctg caccagcgtc tgcggcgtcc gcttcgtggg taacgtcggt 5341 gttcgccagg gcggcagaca gatcctgtgc cgaggcgaag aagtcggcgt cgaccacggc 5401 gccagcattc accgcggtga cgtcgtacag gccgatgttg ccggcggcgg tgctgatcgc 5461 atcgcacgac agcaacaggc gggacacgcg gtccaccgag ttgatgcgca tcaggcgata 5521 cacagagccg atggagtcgg tagcggttgc ctcaacgaag ccgacacgct cacgcaaacg 5581 gccaccgtcg atacgctgag gcgacagggt ttgtgggagc gcgtcagagt tggtgacggc 5641 tgtggatttg gtggttacta ctgccatgat tcattgctcc tgatcaggtt gggagaaggc 5701 caaaagccgc ttaggcttct gcggctgcga tttcaacgac cttctcttct tccacccgca 5761 cagagccgat ggacattttc gcgtagatgc gaacgttgaa gcccttgcct ggatcctcgc 5821 ccaccttggt ggtgatatcg gcgcccttgc ccagcgttac gccagacttg gcccatgcgt 5881 agagcaggcg aacaccacca gacagcgggg tgcgctcgga tggaatccag gtgaagccca 5941 tccacttgcc ttcaacgtcg ccgctttcca ggaacttgcc ggccatgtag tcagcactgg 6001 tcaaggttgg gtcggcgagg atgtcggcag cggcttgcgc cgagtaggtg atgcacagtt 6061 cttcgccgtt gtggttgtcc gcttcgttct ggcggaacag cttgcgggcc tggatgatct 6121 tggccttggt caagccggtg ccgcccacgg cgatcttctg cttggttggc aggaggatgt 6181 tgccggtggt ggagcgggag aagccgccga acgagccgat gattacgtcg tctttggcgc 6241 ggttcagcga ggacaccatg gccttgacgt agtccgaagt cgggtcaacc aacatgcgga 6301 tcttgtcctg gtcgtcgatc atgtcgccgt cttcccagtc gaacaggtcc acaaagcgcg 6361 tgctgtgtgg ctgatcgttg attggggtgt cgccgtggcg agtggtgcgg cgtttggcgg 6421 tacgctggcc caggcggttg atcgacttgg acatgccaac gatgttcggc tcgatggtga 6481 cgtgaccttc aagccgcgag gtcatttgct gtgccagatg acggaagtta gcgccgtact 6541 gctggacaaa ggcttctgtg atttgaaagg acattcgatg cactccaatg cagataaggg 6601 attgcctgcc gggtgtccgc atcgcgggcc ggtattcctg gcgtgcatcg gctttgctgc 6661 gcctggggcg ttccgggtgt ctgcgtgcca tcgcaggccg gcccattgct gggatgcctg 6721 cgatgtttgt gcatgggggg tgtcggtttc ccgactattt ggggctggaa tcagctcagg 6781 cgggacgaag acttgttgta tctgcgctcg tacattgcgt cgagttcagc ctggatgcct 6841 ttgcgtttcg gatcgtgtgc cggcagagcc tgcaactgac tgcgcagttc tgagtcctta 6901 acgtcaaaat cagcctcact tacctgggca ccaccgttga tggccgtgtc ttccttgagt 6961 tccttgccga tgttggctgt aaaggcaatg aagtcaggat cgttgccgta cttggcctgg 7021 agcgtgtcga agttccccgg ctcacctggc ttactggcaa acgcctcggc agcacggaac 7081 gaggcgcgca cgttctgctt catggcttgt tcatcggtcc acacggcctt aagcgcggcg 7141 gtgcaatcct gctgggtcag ctgcccgccg ccctcgatca gcccgggcgc cgccttcatg 7201 tactcgccaa tcacatactg aacctggtca ttggtcaggc ctttggcgtg cgcgcctttc 7261 aaaaacgact gcgtgtcagg gtcggtcttg aactcgtccc agttgaagcc ctcgacgcct 7321 tccagcttga ccgcgtattc ctcggcagtc ttgggcggca catcaccaga acccatgcgg 7381 gtttcaaggt gcttgtacgc ttcggcaacc ttgcggctcg atgcttccag gtcaagggag 7441 ccatcctctt tgttggtccg gtacttctcg gggatgtagt cgttgccggt gttgccgctg 7501 tcaagcacgg ttccggtggc tgtcgtggtg gtcgcagctg tcgtagtggt ggcgccaggc 7561 tcaccctcgg tagtttcagc catgaaaaaa cggcccaggc ggccatggat aaacatgttc 7621 atcgttattc ctcttgctcg ttgggatcgg cttggacgcc gttggcgcgg ttgatacggg 7681 ttacaacgtg gtcgaggact tcacgggccc cggcctgctt gtacgtggtg aggatggcgt 7741 cgatgccacc gatggtgcag gcgttgcgcg agaaccgctg aatcagcagc tccagacaca 7801 tggcgccttc gtggtgttcc tcgaaaacgc gcttgaacat cgcgtcgatc tgctcggggt 7861 tcatggtcat gctgcggctc cctgttgttt caatgcggct tcaccggcct gctgcatcat 7921 tgcttgctgt tgctcctgct ccatcgcggc ctgctgggcc tgggcacggt cggcgcggat 7981 cttgtcccgg tcggcagtgc tgcggatgat cgagccagga acgcccaggg cctcgccctt 8041 gaagcgctgc gcctcgtcca tgtcgatgtt gtccataacg gttgggtcag ttgcggcaat 8101 ggccagcgcg ccagcaacga acgtgtcgat ggcggtcact tcctccagct tctgcgacct 8161 ggccagcggc gaaaggtaac gcacggtgaa gttgcgaccg gccaacgatt caggcgcagc 8221 acccagcaca ccagcgcggt aggcgatgcc gaagcaccgc tcaatcatgg gttgcaggta 8281 ctcggtttgg agtcggccat acaccgggcc cagcagttgg cgaatcaggt tgacccggac 8341 atgcacctca gtggcagtca tcgcggggcc gtcctgggcc tggagctgat cagccatcag 8401 gatcttgcgg atagatccct gcaggcgggc aatcttggtc tcggcgtatt ggaagttgga 8461 gccgctttgc aggggcttca tgctgtccac gctgttggcc acgatgatct tgcgcgggcc 8521 caccttgacg gtgcgcgggt tcaacacgcc gtcgtcctcg gcgatccaca taccggcgat 8581 ggccaggtcc cccgctgcca ggtccatgcg gcacagctcg ttcagggtgc gcgcatcagg 8641 caaggcgtcg aacaccgggc ccacggcgta cacgctgtcc gggatcatca tccagcgcgg 8701 cacgacgacg ggcatttcgt gatagcccga ctcgctaacc agcttcttgg cttccacctc 8761 gaccttgcag gaagcaatgg gcatgttctt cgccaacttg gcgttgacca tgtgcgtggt 8821 gcgcgggtag atcgcgtgga cgaaccgcac catttcctgg ggcttgtcct tcgccagctt 8881 gcgggtgttc tcgcttacgt tctcctcgcc aaactcgttc accgcctgct cagcggtgag 8941 tttgtattcc cggtacacgg tgtcgatctt gccgccagcc ttggacgcag aggcatacac 9001 gcttgcaatg ggccacagat cgaacgtgaa gccgcccttc tccatgtcct ggtcgatgta 9061 cagggcaaac cacccagcgc acacaacgtc gataagcccc tcaaaggccg ctgcgtcgaa 9121 gttggatgcg tggatgttct gccagagaat gtcggccgag tcgtccagcc aacggcgttc 9181 ttcctcggtt tcctggccga cgtccatgcc gaaccacagg gagttggccg gggtcaggcc 9241 cgacatgatc ccggacgaca gaatccgtgc agcgtccgtg gtggtgccgt cgatcatcct 9301 ggccttacgc atctgcgctt caatggccga gatttgttca gtgcaaaagc cactaccccg 9361 gatggggtag ctgtggtcga aacaatcgcg ccagaccgtt tcatgcggca ggcggagaga 9421 cttcaaagtg cccaacgttt tggcgatctg gtctgcattc atgatccgag ggttctcttg 9481 ccttgatcga ggacggaacc cgccgcgcca ccagtggaga gcaggctgct ttcctgtttg 9541 cgcttcttgc gggtggcggt ttcttcgttg gccttctgcg ctgccagatc ggcggctttc 9601 tgtgcctcga cagcagggtc aggcgtggca acggtctttg gtgccttcgg tttgcttccc 9661 atgtcgtcag tccttcgcct tgggttcagg gcacagccag ccatcgttgg tcagcacggc 9721 ctgcttgagg gtggttgcgt cgtggttgcc ggtgctggcc tgggccttgg gcgctggctc 9781 gctggtgcgt tgtgggtcca gcaccagggg atcaccacca gcgttcaggc gctcggcctc 9841 ggtttctgcc tcttccttgc tggcgccgac gaagtcgccg atcttcacga ccttgccgtc 9901 aaccagcggg gctttgtcgt cgatgacgat ccagcgcccg ccgccgttgt gcttggcagc 9961 gaacagcggc tgggccgaag cctgggcgtt agcggtgttg agttcgctgg cgttggggtt 10021 gccggtgctg gcgtcactga ttgcgcctgc aagcggcgtg atggtggcga gcggctcgcc 10081 tggggtctgc ggggtaaggt ctggagctgg catgttcgtg cctcctgtgc tggtgaaggg 10141 atcaggcttc gtcgttgagg tgctgtttga gcgcgtagcc catcagcggc cagaccctgg 10201 cgacagcgtt gtcgcgggcg atcttgcgac caatctccgc gtcgaagttc tcagggctgg 10261 cgcacgcgct ctcaccggtg acggtgaagc cattgcgcag cactaggacg cagaaggtca 10321 gtaacgccaa ctcaccatgc gcgccagtga gtcgggtcaa ctcatcctgc gaattgaagg 10381 cggcctgcac gccatcggca gcagtaaaaa agtactcgcc gacgatgttg gctttcagat 10441 ctgccggagt aacacgcggc gcggtcaagc ccttggcctg aatttcctgc tcgattgctt 10501 ggtctgtaac ggaagtcatg aggtgtggcc cttcgtttga tggatgatca acgagggcca 10561 gaatcaatgg ggtgggctgt cgggttcccg actatttcga ggggctgcgg gagcaagcgc 10621 gggtgatgaa gtcttgcagg ccgatcacct gggcggtgac tttggcgtgg gctgcgacga 10681 ggtcggcata atcctgtcga gcatctgctg caagttcggg ggcggctgca tcagcgctgc 10741 cggtgggctg gggattggct ggcacgcagg cggcacggac gtacacgcgc ttaacaccag 10801 cattgaggtc agtaacaagc tgcggcttgg attgttcagc attgtggatt gcctcctgat 10861 actggctgtc gatggaatcg cggacggtca gagcctgctc gtatgccgct gcctgccctt 10921 ccagcacacc tacacgctcc gttgaggtgg taagcctggc gctgacatgg tcaagacgcc 10981 acaacgccag cagcaggcca atggcgagcg cggcgcagat gccgaggatg attctcacaa 11041 gcccacctcg cacagttcac gctcagcagc gcgacggttg accagccctt tcagcttcgt 11101 acccttggcg tacacccagc ggctcagctc tgcacaggcc ccgcgcacgt cgccggcatt 11161 gagcttgcgc aacagggtgg atgccttgaa ctggggttcg ccgacgttgt acacgaacga 11221 tgccagggcg gcgcggcggg tctcgggctg tggcttggtg acgttgcggt caacgccagc 11281 cattgcgatg gagagttctt gctgcaacag cgcatcgcac tgtgctggtg acggctctta 11341 tacacatctg acgctgccga cgacgcccta cagcgcgagc cgggaggtgg acgcgggcca 11401 gaaaagtaaa cagccaccgc acgaggagca cg //