LOCUS MF417848 6094 bp DNA linear ENV 27-FEB-2018 DEFINITION Uncultured Caudovirales phage clone 10F_2, partial genome. ACCESSION MF417848 VERSION MF417848.1 KEYWORDS ENV. SOURCE uncultured Caudovirales phage ORGANISM uncultured Caudovirales phage Viruses; dsDNA viruses, no RNA stage; Caudovirales; environmental samples. REFERENCE 1 (bases 1 to 6094) AUTHORS van Zyl,L.J., Abrahams,Y., Stander,E.A., Kirby,B.M., Clavaud,C., Farcet,C., Breton,L. and Trindade,M.I. TITLE Novel phages from South African skin metaviromes JOURNAL Unpublished REFERENCE 2 (bases 1 to 6094) AUTHORS van Zyl,L.J., Abrahams,Y., Stander,E.A., Kirby,B.M., Clavaud,C., Farcet,C., Breton,L. and Trindade,M.I. TITLE Direct Submission JOURNAL Submitted (29-JUN-2017) Biotechnology, University of the Western Cape, IMBM, C2, Life Science Building, Robert Sobukwe Rd, Cape Town, Western Cape 7550, South Africa FEATURES Location/Qualifiers source 1..6094 /organism="uncultured Caudovirales phage" /mol_type="genomic DNA" /isolation_source="healthy skin from forearm of human male" /db_xref="taxon:2100421" /clone="10F_2" /environmental_sample gene complement(60..470) /locus_tag="10F2_1" CDS complement(60..470) /locus_tag="10F2_1" /note="Similar to YP_009104242.1 [Idiomarinaceae phage Phi1M2-2] (BLASTp score : 64.7). Similar to PB005964 [Pfam-B_5964] (hmmscan score : 41.9)." /codon_start=1 /transl_table=11 /product="putative structural protein" /protein_id="ASN67498.1" /translation="MPVQSTNPDYDAHIEEWRMMDDALEGEGAIKRSPRNLPKPSGMT EAEKLDGAGNAYLYQNYTARAQYEHWVRDSLRSMMGLVSRLIPEVKLPAGLKGLEDNA TADGFGLTQLFLRIVWQAIFHGRSPVVVNIDDAV" gene complement(470..1960) /locus_tag="10F2_2" CDS complement(470..1960) /locus_tag="10F2_2" /note="Similar to YP_006590034.1 [Cronobacter phage phiES15] (BLASTp score : 808)." /codon_start=1 /transl_table=11 /product="putative terminase large subunit" /protein_id="ASN67499.1" /translation="MKPEHMKLLRDRFWRLNNLYFITDKQGKKVRFRMTQEQVDYFQG MHTRNLILKARQLGFTTLVCIVQLDAALFEAAKCALIAHTLNDAKRLFREKIKYAYDH LPKEIKAANPASNDAAGELVFSKGGSLYVSTSFRGGTLRYLHVSEFGKICAKFPHKAR EIVTGAFEAVAAECFVTIESTAEGRAGYFFDYSQSAEKQQLAGVPLGLLDWKFFFFSW WRNPLYWLDPTDVTIPDRLTKYFDELSAKHGIVTNPGQRAWYSAKEKTLGDDMKREYP SIPAEAFQQTIEGAYYAKQFTKLYAAQRIGKLPDNSHLPVHTFWDIGVGDSTAIWFVR IVGEEYHVVDFYQNSGEGLRHYMKVLKDRGYAYGEHWGPHDIDNREFGSDGKTRRELA REGYEIDGQVYRMTFQVVPKLGVDEGIEQAREILPNCAFDEAKCSEGITALESYRKEW DDKRGCWKDKPLHDWSSHPADGFRYFAVAMARRKRTGGVRRIGGLA" gene complement(1963..2481) /locus_tag="10F2_3" CDS complement(1963..2481) /locus_tag="10F2_3" /note="Similar to YP_004934031.1 [Escherichia phage HK639] (BLASTp score : 70.5)." /codon_start=1 /transl_table=11 /product="putative terminase small subunit" /protein_id="ASN67500.1" /translation="MASRKSIDWESVESSYRVGSPSIRALADAHGLTEGAIRKRAAKE GWAGDLTEKVRIATKEKLVRTKVRSESTQRTDAEIVEDESEQRAGLVLAHRTGLANWR AIADKLSVALAEIDVNEENLGDFSRALNAGVDAQLKVIKGERQAYNLDAEEGDKTVDT LASLMDELSKDA" gene complement(2612..3010) /locus_tag="10F2_4" CDS complement(2612..3010) /locus_tag="10F2_4" /note="Predicted by MGA" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN67501.1" /translation="MSTKQPDWEAIYDNCAKAGIPGVVYHHLNRGGEAFLSGVFRGLL AAADANLKIFDGQVPEIIEAIPEFSVPRGRVDYLLIHVDGSITVCELKDGSKGKQHVL SGLGQCIAYAVQVGMARAGIPIIRKALVFC" gene complement(3020..3454) /locus_tag="10F2_5" CDS complement(3020..3454) /locus_tag="10F2_5" /note="Similar to YP_009103949.1 [Shewanella sp. phage 3/49] (BLASTp score : 111)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN67502.1" /translation="MTTIAYKDGVIAYDSRQTRSGSIVSDDCQKLTVVDGVSFFLSGA VCDEKALIAAYFGTPSPVPVECSGYVVDGGRLQMVGHDDKTGVWRQDLDPANPDAIGS GSAYALAAMDMGASAEEAVRAAMKRDIYTGGKVRTIRIARDQ" gene complement(3451..3615) /locus_tag="10F2_6" CDS complement(3451..3615) /locus_tag="10F2_6" /note="Predicted by MGA" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN67503.1" /translation="MASVTAHVVCRQRWWLTYYLAGVLALAQLTRREPCLERVSYWVG RGIEIEVHPE" gene complement(3666..3947) /locus_tag="10F2_7" CDS complement(3666..3947) /locus_tag="10F2_7" /note="Similar to PF05449.6 [DUF754] (hmmscan score : 90.8)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN67504.1" /translation="MVAVIQAALCAVIFVMIGLRYRPYPDARYKLGVSLMAWAACAVT GMQCVSLIGRMVLHDEFADVSWFNTAFYLLAAILVCRAKGNVAKIVRVD" gene complement(3948..4322) /locus_tag="10F2_8" CDS complement(3948..4322) /locus_tag="10F2_8" /note="putative Orf89; Similar to NP_061583.1 [Pseudomonas phage D3] (BLASTp score : 102)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN67505.1" /translation="MAEPSTGALAVTGVLASVGLGAFFPELDLATLVGAFGGAFFYVV YARDIGMLRRIGYLLAGWIGGYLGAAEMLGRAWTQTAGFSAFVCGVLCVVTFSGLLEW MQTGQMPRWLQWVFRLRARKEG" gene 4416..4712 /locus_tag="10F2_9" CDS 4416..4712 /locus_tag="10F2_9" /note="Predicted by MGA" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN67506.1" /translation="MGHFVITFRFEYDGSYQERYDSFTKKVREIASAQWEETSSFYAI EASGTAESIRDTLYFQTGFMESKDQMVVIDLDNRVKAIKGNVKYTSTLARCLGF" gene complement(4689..4901) /locus_tag="10F2_10" CDS complement(4689..4901) /locus_tag="10F2_10" /note="Predicted by MGA" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN67507.1" /translation="MKSKYRCLVEDILSCEQKLREAKVIQRDAQEKLTQANGFVSYQE DNLRENERKLAEIEAVLFSSEAQAPR" gene complement(5145..5654) /locus_tag="10F2_11" CDS complement(5145..5654) /locus_tag="10F2_11" /note="Similar to PF09411.5 [PagL] (hmmscan score : 145.8)." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="ASN67508.1" /translation="MKTVLAAAALSLFAASAGAAELSGALGATSQGGITARAGVGFNW DKSWFESSTGRLTGYWDAGYTYWEAGDASGGAHSLSFAPVFVYEFGSGNVKPFVEAGI GLAVFSGTSAGDQDFGSAFNFEDRIGAGLKIGETQKVGIRAIHYSNAGIKQPNDGIES YSLFYSHQI" ORIGIN 1 gcgggggagt cggggtggcg gtgagtgggg tagagcacgc aggtggaaac gtaatatgac 61 tagacagcgt catcgatatt gaccaccacc ggagaccggc catgaaagat ggcctgccaa 121 acgatccgca ggaatagctg ggtcaggccg aagccatcgg cggtggcgtt gtcctccagg 181 cccttcagac ccgcaggcag cttcacctcg gggatcagcc gggaaaccag gcccatcatc 241 gagcgcagcg aatcccgcac ccagtgttcg tactgggccc gggcggtgta gttctggtac 301 aggtaggcat tgccggcgcc atcaagcttc tcggcctcgg tcatgccgct gggcttgggc 361 aggtttcgcg gactgcgctt gatagcgccc tcgccctcca gagcgtcgtc catcatccgc 421 cactcttcga tgtgagcgtc gtagtctggg ttggtggatt gaacaggcat tacgccaaac 481 ctccgatgcg gcggacaccg cctgtgcgtt tacgtcgcgc catggcaacg gcgaaatagc 541 ggaacccgtc agccgggtgc gatgaccagt cgtggagcgg cttgtccttc cagcacccgc 601 gcttgtcgtc ccattccttg cgataacttt ccagggcggt gatgccctca ctgcacttgg 661 cctcgtcgaa agcgcagttg ggcaggatct cgcgcgcctg ctcgatgcct tcgtcgacac 721 cgagcttcgg caccacctgg aaggtcatcc gatacacctg gccgtcgatc tcgtagccct 781 ctcgcgcgag ttcgcggcga gtcttgccgt cgctaccaaa ttcccggttg tcgatgtcgt 841 gggggcccca gtgctcgccg tatgcatacc cgcgatcctt cagcaccttc atgtagtgcc 901 gcaggccttc tccgctgttc tggtagaaat cgacaacgtg gtattcctcg ccaacgatcc 961 ggacaaacca gatagcggtg gagtcgccca cgccgatgtc ccagaaagtg tgcaccggca 1021 ggtggctgtt gtccggcagc ttcccgatac gctgggcggc atagagctta gtgaactgct 1081 tggcgtagta ggcgccttca atcgtctgct ggaatgcctc tgcagggatc gacggatatt 1141 cccgcttcat atcgtcgccg agggtctttt ccttggcgct gtaccaggcg cgctggcctg 1201 ggttggtgac gatgccgtgc ttggcgctca gctcgtcgaa atacttggtc aggcgatctg 1261 gtatcgtgac gtcggtcggg tccagccagt acagcgggtt gcgccaccag ctgaaaaaga 1321 agaacttcca gtccagcagg cccaagggca caccggctag ctgctgcttc tcagcgctct 1381 gcgagtaatc gaagaagtaa cccgcccgcc cctccgccgt cgactcgatc gtgacgaaac 1441 actctgcggc gacagcctca aaggcgccgg tgacgatctc tcgggctttg tgcgggaact 1501 tggcgcagat cttcccgaac tcggatacgt gcaggtagcg cagcgtgccg ccccggaagg 1561 aggtcgatac gtacagggac ccgcccttgc tgaacaccag ttcgcctgct gcgtcgttgc 1621 tggcagggtt ggcggccttg atctccttgg gcaggtgatc gtaggcgtac ttgatcttct 1681 cgcggaacag gcgcttggcg tcgttcaggg tgtgggcgat cagggcgcac ttggcagcct 1741 cgaacagcgc ggcgtcgagc tggacgatgc aaaccagcgt ggtgaaccca agctgccgag 1801 ccttgaggat gaggttccga gtgtgcatcc cctggaaata atcgacctgc tcctgcgtca 1861 tgcggaagcg gaccttcttg ccctgcttgt ccgtgatgaa gtacaggttg ttaagccgcc 1921 agaaccggtc ccgaagcagt ttcatgtgct cgggcttcat ggtcaggcgt ccttcgatag 1981 ttcatccatc aggctggcca gggtatcgac cgtcttgtca ccctcctcgg cgtcgaggtt 2041 gtaggcctgg cgttcgccct tgatcacctt gagctgggca tcaacgccgg cgttcagtgc 2101 acgcgagaag tcgcccaggt tctcttcgtt gacatcaatc tctgccagag ccacagacag 2161 cttgtcagca atcgcccgcc agttggccag gccagttcgg tgagcgagaa caagaccagc 2221 acgctgctca gactcatcct cgacaatttc cgcgtcagtg cgctgcgtac tttcgctgcg 2281 tactttcgtg cgtaccagct tctccttggt ggcgatcctg accttttcag tcaggtcgcc 2341 agcccagcct tccttcgctg cgcgcttgcg tatagctccc tctgtaaggc cgtgagcgtc 2401 ggccagcgcc cgaatggatg gcgacccaac acgatacgag gactctacag actcccagtc 2461 aatggacttt ctcgatgcca ttgccgatgt acccttcgat gaagcgcatg gccgaatctc 2521 ggtgcgcctc ctcagagccc ataggcaccg cgataacgcc agcggatcga cacgcgtcga 2581 taactagaag ctcctcgtcc gccccccccc atcagcagaa cacaagagcc ttgcgaatga 2641 tgggaattcc ggcccgagcc atgcccacct gcactgcata ggcaatgcac tgtccgagcc 2701 cggaaagaac atgctgcttt cccttcgatc catccttgag ttcgcaaacc gtgatggacc 2761 cgtcaacgtg gatcagcaga tagtcaactc gaccgcgcgg aacactgaac tcgggaatgg 2821 cctcgatgat ctcgggcacc tgaccgtcga agatcttcaa gttcgcatca gctgcagcga 2881 gaagcccacg aaagacaccg gacaagaaag cctcgcctcc tctgttgaga tggtgataga 2941 caacaccagg aatgccagct ttcgcgcagt tgtcgtaaat cgcctcccag tcgggttgct 3001 tggtgctcat gtcgaatcct tactggtcgc gggcgatccg aatggtgcgg accttcccgc 3061 cggtgtagat atcccgcttc atcgcggcac gaaccgcctc ttctgcgctt gcgcccatgt 3121 ccattgctgc cagggcatag gccgagccgc tgccgatcgc gtcagggttt gccgggtcaa 3181 ggtcctgccg ccatacgcca gtcttgtcgt catggcccac catctgcaac ctgccgccat 3241 ccaccacgta gcccgagcac tcgacaggta ccggcgatgg cgtgccgaag taagctgcaa 3301 tcagggcctt ctcgtcgcat acggcaccgg acaggaagaa gctgacgcca tccacaacgg 3361 tgagcttttg gcagtcatcg gaaacgatgg agccactgcg ggtctggcgg gagtcgtagg 3421 cgataacgcc atccttgtag gcgatggtgg tcattcaggg tgaacctcga tctcgatgcc 3481 gcggcccacc cagtagctga cacgctccag gcatggctca cgacgggtca gctgtgccag 3541 ggccagaaca ccggccaagt agtatgtcag ccaccaccgc tggcggcaga cgacatgagc 3601 agttaccgaa gccattggcc attcctcgcg cctcgaaacg gcgaatctcg aatttgtggc 3661 gcgggtcagt caacgcgcac gatcttggcc acgttcccct tggcccgaca caccagaatg 3721 gcggccagaa gatagaacgc agtgttgaac cacgacacat cagcgaactc gtcatggagc 3781 accatgcggc cgatgaggct gacacactgc atgccggtca ccgcgcaggc agcccaggcc 3841 atcagggata cgcccagctt gtagcgggca tcggggtatg gccggtagcg caagccgatc 3901 atcacgaaga tgacggcgca cagtgcggcc tggataacgg caaccattca accctccttc 3961 ctggctcgca ggcggaagac ccattgcaac cagcgtggca tctgccctgt ctgcatccac 4021 tccaacaagc cggagaacgt gaccacacag agaacgccac agacgaaggc gctgaagcct 4081 gctgtctggg tccaagcccg gccaagcatc tcagcagcac cgaggtagcc gccgatccag 4141 ccggccagca ggtagccaat gcggcgaagc atgccgatgt ctcgggcgta caccacgtag 4201 aagaaagccc cgccgaacgc gcccaccagg gtggccaggt ccaactcagg gaagaaagca 4261 cccaggccga cgctggcaag tacgccggtc actgcgaggg cgccggtact cggttcggcc 4321 atatggatgc tccatgaaga aaaaacccgc acagggcggg tagtggcttc gtgctatgtt 4381 tcaaggctct cacacccgca acaaaggact tagcaatggg acacttcgtg attacgtttc 4441 gatttgaata tgacggcagc tatcaggagc gatacgactc gttcacgaaa aaggttagag 4501 aaatagcgtc cgcccaatgg gaggagacat cttccttcta cgcaattgag gcatccggca 4561 cggcggaaag catccgggac acgctctact ttcaaactgg atttatggag tccaaggacc 4621 agatggtggt catcgactta gacaaccgag taaaggcgat caaggggaac gtcaagtaca 4681 ccagcactct agcgaggtgc ctgggcttct gagctaaaca agaccgcttc gatctcggcc 4741 agcttgcgct cgttctcacg aaggttgtcc tcctgatagg aaacgaagcc gttcgcctgg 4801 gttagctttt cttgcgcgtc acgctgaata accttggcct ctcgcagctt ctgctcgcag 4861 ctgaggatat cctcgacaag gcagcgatac ttgctcttca tgctgacctc cagatacgaa 4921 aaagccccgg cagataccga ggcttggaat gggtgcggag ggccggtgca tacccggctt 4981 ggttggcctg gatcgctggg tcacataccc cagactctca tcgcgtagcc gatcagggag 5041 cgcacggctt tgatcgacgc cactaccgac ttagcccagc tgcctgagcg tgtcatccgc 5101 ataaaaaagc ccgcacaggg cgggcaaaga gggatcgtgc ttttttaaat ctggtggctg 5161 tagaacagcg agtacgactc gataccgtcg ttgggctgct taatgccagc gttggagtag 5221 tgaatcgctc ggatgccaac cttctgcgtc tcgccgatct tcaagcccgc accgatgcgg 5281 tcttcgaagt tgaaggccga accaaagtcc tggtcacctg cggacgtacc agagaagacc 5341 gccaggccga tgccagcctc aacgaatggc ttcacgttac cgctgccgaa ctcgtaaacg 5401 aaaactggcg caaaggacag cgagtgagcg ccaccggaag catctcctgc ttcccaatag 5461 gtatacccag catcccagta accggttaga cggccagtac tggattcaaa ccagcttttg 5521 tcccagttaa agccaacgcc tgcacgcgcc gtaatgccgc cttgacttgt cgcacccagc 5581 gcaccggaaa gctcagctgc tccggcagac gcagcaaaaa gggacagtgc tgcagcagct 5641 agaacggttt tcataatcac ggtcttccat gtttgtttag ttagcaacct atcagaatca 5701 tagcgccatc aaaacgttcc gtgctacaca aaaaaaatct gacctcgcca gaggtggcgt 5761 cgggcctctt gaggccctct tcgggcaata aaaaacccgg ctcattggcc gggtttcttt 5821 tcgtcactcc tcaacacgcg caggaatgac aggatggagc aaatttacga catggcgaca 5881 tgatattgca agcccttttg aggggcatat tacgccgcct cgtcgaacag cacgccaatc 5941 gctgcaagca tgtactaggt ggtagccgcc tcattaaaca acagaagcac aacaaacaac 6001 acacaaccag caaacacata tcgacagaac agagcagcaa acaaacaaac aaacacaaca 6061 cgaaaaacga ctacaaacaa caacaatcaa ccaa //