LOCUS LC425521 15089 bp DNA linear ENV 25-JUL-2019 DEFINITION Uncultured phage DNA, contig: NODE562. ACCESSION LC425521 VERSION LC425521.1 KEYWORDS ENV. SOURCE uncultured phage ORGANISM uncultured phage Viruses; environmental samples. REFERENCE 1 AUTHORS Morimoto,D., Tominaga,K., Nishimura,Y., Yoshida,N., Kimura,S., Sako,Y. and Yoshida,T. TITLE Co-occurrence of broad and narrow host-range viruses infecting the toxic bloom-forming cyanobacterium Microcystis aeruginosa JOURNAL Appl. Environ. Microbiol. 85 (2019) In press REMARK DOI:10.1128/AEM.01170-19 Publication Status: Available-Online prior to print REFERENCE 2 (bases 1 to 15089) AUTHORS Morimoto,D., Tominaga,K., Nishimura,Y., Yoshida,N., Kimura,S., Sako,Y. and Yoshida,T. TITLE Direct Submission JOURNAL Submitted (21-SEP-2018) Contact:Daichi Morimoto Graduate School of Agriculture, Kyoto University, Laboratory of Marine Microbiology; Kitashirakawa oiwake-cho, Sakyo-ku, Kyoto, Kyoto 606-8502, Japan FEATURES Location/Qualifiers source 1..15089 /organism="uncultured phage" /mol_type="genomic DNA" /host="Microcystis aeruginosa" /db_xref="taxon:278008" /environmental_sample /country="Japan: Kyoto, Hirosawanoike Pond" /collection_date="2017-10-19" /note="contig: NODE562" CDS <1..545 /note="MVG_NODE562_1" /codon_start=3 /transl_table=11 /product="hypothetical protein" /protein_id="BBG57988.1" /translation="NKCIKQLQEEYQELEKNKEYSQTWVDNLKQRVHDLECAVHLLQQ ENKQLKNQSKTKPEPQSEPKPEKTLAANKGKKSKFKLPENFADYQQECDDLINALSCF YNIKKGKWGKDILQFILTPTDVQKENQLHPYPDKWKAGLYLHYGYWSMDKVDLSDPDE WEEWLMDIYDFADSNDIEIS" CDS 663..809 /note="MVG_NODE562_2" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="BBG57989.1" /translation="MDNVKQLIEATQTPPEIKKARIFCKEIELFAQSLNQEAQTVLDK YPAL" CDS 867..1136 /note="MVG_NODE562_3" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="BBG57990.1" /translation="MITKAEYQRFKIGEAKHQELFSEKKPLLSFSSLRKIIAGYYPVI VDEKDGYYYYYHIEWIRQCSVIQSKSNGVICPLRLLIPSIFSVIW" CDS 1208..1762 /note="MVG_NODE562_4" /codon_start=1 /transl_table=11 /product="serine/threonine protein kinase" /protein_id="BBG57991.1" /translation="MRQFTETLPNQVALEMITVPARTFAIGKYPITQAQYEAVMGTNP SYFINNLQNPVENLCWYDARSFCKKLSQITGKNYRLPSNTEWEYACRAGTTTRFYFGN DFNQLGDYAWYYDNSQGTTHPVGQKLPNDWGLYDMSGNVWERTQEAKLRGGSWYSDPT YCYSAYICNLTLAESIGFRVVCDK" CDS 1819..2007 /note="MVG_NODE562_5" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="BBG57992.1" /translation="MLQDIASYRLSFLKMTELRKLASEYGLPKQRWNRTILIVELSKI VDWTTLPKPKIVIDLFEF" CDS 2066..2272 /note="MVG_NODE562_6" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="BBG57993.1" /translation="MQDTAYLVVRGKTIKVLRRSAKYGIKLRLRRSGELGIFLSDESQ YLGLDEELAKTRLNELLEGKVIYL" CDS 2333..2548 /note="MVG_NODE562_7" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="BBG57994.1" /translation="MQKKNENGSPKNQTFTVAAIIRDKKGVRKEYSHNINTEADVTEI FEDYNQRDDLLYFEAIDVKTGQIIKLK" CDS 2568..2936 /note="MVG_NODE562_8" /codon_start=1 /transl_table=11 /product="hypothetical protein MaMVDC_61" /protein_id="BBG57995.1" /translation="MLSFQEFQEQILGIFDVTEKKCKFANNCTYLGATISYDNYQVVF EVKYIKSNGRWHIQKFLAQGCETFSDSLTQGIKTIGKQTTDSINGELQVFHKIQEGWR KAKFQELFESFDELTKTENK" CDS 2946..3218 /note="MVG_NODE562_9" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="BBG57996.1" /translation="MLSFQEFQSTIKKNIPYYLWEFEQKQSSDNNDEYWAKIKNSQIG VRYLCRLNKFIVILQNNSKDYGDQTTITTDIKFIHDAVVSYIQSGF" CDS 3220..3717 /note="MVG_NODE562_10" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="BBG57997.1" /translation="MMIRFYWNDKLVSCHETQEEAFEQGFKYLHQYPALSDFKRMPHK QCAFVDTTELDWWKHSKILFERFTDWIWYKRYSDNRGVLMDMKRILSEIKRKGYLSLD DMALLTEVNPNFLDSFAKCYKLTTEEVKILVSETKIPFDMVFEYIEIRYSALAYWLRK SKITP" CDS 3775..4173 /note="MVG_NODE562_11" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="BBG57998.1" /translation="MINWNLGRQLAIESFDEMVGEFAQEINFQIEDTKWDWPRETVRK NGSVVGSPRDIVDTGELKNSQFIEDVSDVYKLIGYTADHAALVHEGYQVERNDGTVTD VPARPFIDTAIEDYNPIEAYSEILKEKLND" CDS 4678..5412 /note="MVG_NODE562_12" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="BBG57999.1" /translation="MTNQILELNRSDNLTPSRDTRFFISGTYGFGEDPPTRVADLAAA IVLGDTTLTVATGGFGRILYAGTLIYIGASSSDYVVVRTKTTTLTQTAIQIEPAKVAA SLATPAQKCTLKSWVPFISAKTFNVDTSTTEVTDSVFTEIAVEKFISEIMSTGSVSGA LVFGDPGYEIIKAAEQKGQRIYLEIIYMGQRGGLGFQTNVSQNVSGEKGNFLQGNVTL TISGNVIDIKPMATSPFSPNVADDLN" CDS 5652..6437 /note="MVG_NODE562_13" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="BBG58000.1" /translation="MAKLSVLGKVKFNETFFFPLKKEWLYYIQDNDALLEKIDAIATE ENGEIGIKFLKRYGINPKENESVKEYLEAREKADKAYLEKIKAIGQKTGLSTTEIEGV VVNDGSIRERIEQVMVDALDGVKSDSVEQKVETAAIVQQSILNNRKKTRELTRESIEL VEPYLDELNSLFKDRETTYETYNKALLANFLGSPRRVVKLKDKSAIEFTIEDINDMSQ FMVVKLYQDYLWQDITQWQNPEPEKPEPEKTESEPTEDDEKNE" CDS 6644..6883 /note="MVG_NODE562_14" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="BBG58001.1" /translation="MVHAYLIAQSKEGSKSQNLNPSDFLPYQFKENKKYFLDRETAQI LLEAMKVGRVPVFATQIIVDCGLYDEIIQLIGEKS" CDS 10895..11029 /note="MVG_NODE562_15" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="BBG58002.1" /translation="MSLFNKKLTKLKRIKELNRLKNGNLLKNKRKNDNKKQKKGYKKK " CDS complement(11304..11519) /note="MVG_NODE562_16" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="BBG58003.1" /translation="MLTPPDTAKAVGSNGKAKGAKDANAGNKGLDIAVMPKSIPKPSL ATVSLSLGSLLIFRLIRLLILRKNLSK" CDS complement(11949..12446) /note="MVG_NODE562_17" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="BBG58004.1" /translation="MFVVPFAMFVNRSFSVLKTSSITSAKIPDINGVKVIKPVFHAPI INPCILLTTGAILGIAKMVDSPMPTCATTPIVNAPASCEIIRKLSLKDVTIPDARLVI PANIPPKMSVKFRSNLNKAVSIEASALVKSLVPFSAAAPNSCSRIFVTFGKTSSDKSE PFATN" CDS complement(12513..12923) /note="MVG_NODE562_18" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="BBG58005.1" /translation="MLAPIFCSDSFKLACKGFVKSLLAKAINPRATKALRAILRATKA PLAIALLANKVSNTPPKLLALVAAVHIAPLIPLKVNHSLLSKGTKNICPNTISCSNIK VKAVSSKTPLGIDLKRLPNVFPIDCIGLTAFSQL" CDS 14848..>15089 /note="MVG_NODE562_19" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="BBG58006.1" /translation="MADLIRQTNKELGFIPQSIKDIVTNLNTLPEPIKLINSELVAIP PNIKTSGEDLVKSIEETAEAIRKAKEGLILPAPGNF" ORIGIN 1 tcaataagtg cattaaacaa ctacaagagg agtatcaaga gctagagaaa aataaagaat 61 acagccaaac gtgggttgac aatttaaaac aacgagttca cgacttggaa tgtgcagttc 121 atctattgca acaggaaaat aagcaactaa aaaatcaatc aaaaaccaag ccagagcctc 181 aatcagaacc taaaccagag aagacactgg cggctaacaa gggtaaaaaa tctaagttta 241 agctaccaga aaactttgct gactatcagc aagagtgcga tgacctaatt aatgccctat 301 cttgcttcta taacatcaaa aaaggcaagt gggggaaaga tattctccag tttatcctca 361 cccctaccga tgttcaaaaa gagaatcagt tgcatccata ccctgataaa tggaaagcag 421 gtttgtattt acactacggg tattggtcaa tggacaaagt tgatttatct gatcctgatg 481 aatgggaaga atggctcatg gatatttatg actttgctga cagtaacgat atagagatta 541 gttagtttcc agtcatcagt tatcagtagt acacttgttc aaaaaagatt ctcccagata 601 cttgacattt atgggagaat cggatatatt aaaagagtaa acaaaacgca gggaacaaga 661 ttatggataa cgtcaaacaa ctaatcgaag caacgcaaac tccacctgaa attaaaaagg 721 ctcgaatttt ctgcaaagag attgagttat ttgctcaatc tttaaatcag gaagcacaaa 781 cagtgctaga caaatatcca gcgttataat cagttatcag ttatcagtag tcagttacaa 841 aatatcaatc actcaagagt agcaaaatga taactaaagc agaataccaa cggtttaaga 901 taggcgaagc aaaacaccaa gagttatttt ctgagaaaaa acctctttta agtttttctt 961 cgctaagaaa aataattgct ggatactatc cagtaatagt tgacgaaaaa gacggttatt 1021 actactacta tcatattgag tggatcaggc agtgtagcgt tattcagtct aaaagtaacg 1081 gtgttatttg tccactcagg ctattaatcc catctatctt ttcagtaatt tggtgaaaca 1141 gtaatcagta gtcagttatc agttatcagc tatcatctgt caaaaacaaa acacttagga 1201 gtaaaaaatg agacaattta ccgaaaccct gcctaaccag gtcgcattag aaatgataac 1261 cgtgccagca agaacctttg caattgggaa atacccaata actcaagcgc agtatgaagc 1321 cgttatgggt acaaatccct cttactttat aaataatctc caaaatcctg tagaaaatct 1381 ttgttggtac gacgctcgat ctttttgtaa gaaattaagt caaataacgg ggaaaaacta 1441 tcgcctacct tctaacacag aatgggaata cgcttgtcgt gctggtacta ctactcgctt 1501 ttatttcggt aatgatttta atcagttagg tgattacgct tggtattacg acaattctca 1561 gggaacaact catcctgtag gacaaaaatt acctaacgat tggggactct atgacatgag 1621 tggcaatgtt tgggaaagaa ctcaagaagc caaattgcgg ggcggttctt ggtacagcga 1681 tcctacttat tgctattctg cttatatctg taacttaaca ttagcagaaa gcatcggttt 1741 tcgggtagtt tgcgacaaat aatcaagatt cagttatcag ttatcaggtg ccatcgacaa 1801 cttaacagga gtaaaaatat gctacaagat atagctagtt atcgactaag tttcttaaaa 1861 atgaccgaat taagaaagtt agcttctgaa tatggattac caaagcaacg atggaaccga 1921 acaattttga tcgtcgaact tagcaaaatt gttgactgga cgacactgcc aaaacctaaa 1981 atagttatcg acttgtttga attttagttc tgagttatca gttgtcagtt accagcaact 2041 agaagtaaat atgaattaag cgcatatgca agatactgct tatctagtag ttcgcggcaa 2101 gacaattaaa gttttgcgta gatccgcaaa atatgggatt aaactaagac tgagaagatc 2161 gggggaatta ggaatatttt taagcgacga aagccagtat ttaggattag atgaggaatt 2221 ggctaaaacc cgtttaaacg aattacttga gggaaaagtg atttatttgt aagttatcag 2281 ttatcagtta tcatcaacaa atcaacagaa gtaattatgt ttcacttgaa ctttgcagaa 2341 aaaaaatgag aatggcagtc ctaaaaatca gacttttact gttgcggcta ttatacgcga 2401 taagaaggga gtgcgcaaag agtattccca caacataaat acagaagctg atgttactga 2461 aatttttgaa gattacaatc aacgagacga tttattgtat tttgaggcta tagacgttaa 2521 aactggtcaa attattaaac taaagtaaca aatcaacaga ggcaattatg ctatcatttc 2581 aagagtttca agaacaaatt ttaggtatct ttgacgtaac cgaaaaaaaa tgtaaatttg 2641 caaacaattg cacttatttg ggggcaacta ttagttacga taactatcag gtagtatttg 2701 aagtaaaata catcaaaagt aacggtcggt ggcatattca aaagtttctg gcgcaaggat 2761 gtgagacttt ttcagattcc ttgactcaag gtatcaaaac catcggcaaa caaactacag 2821 actctattaa cggtgagttg caagttttcc ataaaattca ggaaggatgg agaaaagcta 2881 aatttcaaga gttgtttgag tcttttgatg agttaacaaa aacagaaaat aaatagagga 2941 gaattatgct atcatttcaa gagtttcaat ctacaatcaa aaaaaatatt ccctattatc 3001 tttgggaatt cgagcaaaaa caatcttctg acaataatga cgaatattgg gcaaaaataa 3061 aaaattcaca aataggagta cgttatcttt gtcgattaaa taaatttatc gtcattctgc 3121 aaaataacag caaggattat ggcgatcaaa caactataac tacagatatt aagtttattc 3181 acgatgccgt cgtgagctat attcaatcag ggttttagag tgatgattag attttactgg 3241 aatgataaat tagtgtcttg ccacgaaaca caagaagaag cttttgagca aggattcaag 3301 tatttacatc agtatcctgc attgtctgat tttaagcgta tgccccataa acagtgtgca 3361 tttgtggata caacagaact tgattggtgg aaacattcaa aaatcctttt tgaacggttc 3421 actgattgga tttggtataa gcggtattct gataatagag gggtacttat ggacatgaag 3481 cgtatccttt cagaaataaa aagaaaagga tatttatctt tagacgatat ggctctgtta 3541 acagaggtta acccaaattt cttagatagc tttgcaaagt gctacaagtt aaccacagaa 3601 gaagtgaaga tattggtatc tgagacaaaa ataccatttg acatggtttt tgagtacata 3661 gaaattcgtt attcggcact agcgtattgg ttacgcaaat caaaaatcac tccctaaaac 3721 caataaaaat aaattgttat aatagctgta agttatcctt acagctattt tttaatgatc 3781 aactggaatc taggaagaca attggcgatt gagtcttttg atgaaatggt gggcgaattt 3841 gcccaagaaa ttaactttca gatagaagat accaaatggg attggccacg ggaaaccgtg 3901 cgaaaaaatg gtagtgtagt tggctcaccc cgggacattg tagatacagg tgagctaaaa 3961 aatagtcaat ttattgaaga tgtgtctgat gtttataaat taatcggtta cacggctgat 4021 catgccgctc tcgttcatga agggtatcag gtagagcgta acgatgggac agtaacagat 4081 gttcccgccc gaccttttat tgacacagcc atagaagact ataatccaat tgaagcttat 4141 agtgaaatct taaaggaaaa attaaatgac tgaatcagga ctaagagacg ttttattagg 4201 tattagaaat aacttaaaga tacttatcgg taatgactta ggcaaatatg aaataacaag 4261 ccctacaggg caaaaattaa acgaaataga cgctatctgg gtagaaccgc ccgaactacc 4321 cgctaactat aaagtaaagc ctaatagcgg gattgaggca attatacaga gggaacccga 4381 cccctatcac gaaaatctat taggatacac cgtaggcata aataactatt gtattacttt 4441 aaaacagtac aacttagaga aatccttaac gccggtaatt gaaagactta aatcatcttg 4501 ctactggaat tttctagatc agccccgatt aaccccctac accaaaacct ctgaaggaat 4561 cattagacca aaagcgacct ttaaaatcac tactgctagg cttttagagc tttagagtac 4621 acatttacta atcttttaga gtacaatatt actagaaaag tttagtcagt gattagaatg 4681 accaatcaga ttttagagtt aaaccggagt gataatctta caccctcccg tgatacgcga 4741 ttttttatct ctggtactta tgggtttgga gaagatcctc cgacgcgggt agctgattta 4801 gctgccgcga ttgttttagg cgataccacc cttaccgtgg ctactggagg ttttgggcgt 4861 attttatacg ctggtacgtt aatttatatc ggagctagta gtagcgatta cgttgtggtt 4921 cgcaccaaaa ctacgacact aacccagaca gcaattcaga tcgagccggc taaggttgct 4981 gcatctcttg ctacccccgc gcaaaaatgc acgcttaaat cttgggttcc gtttataagt 5041 gcaaaaacct tcaacgttga tacttctact accgaggtaa ctgatagtgt cttcaccgaa 5101 attgcggttg aaaaattcat ttccgagatt atgagtaccg gatcagtgtc gggtgcgctt 5161 gtatttggtg atcctggata tgaaattata aaagccgcag agcagaaagg tcagcgaatt 5221 tatctcgaaa ttatctacat gggacagcgc ggcgggttag gattccagac gaatgttagc 5281 caaaatgtta gcggagaaaa aggcaacttc ttacaaggaa acgtgactct aactattagt 5341 ggcaacgtga ttgacattaa accgatggct acaagcccgt ttagtcctaa tgtagctgat 5401 gacctcaatt aaagttaacc ttcttgttga tgaaaacgaa gaggtaatgt tagttaactg 5461 caaaataatt aataattacc tcttattttc tctcggtacg tttgatcgag aaataagtca 5521 acaagaaaaa acattaatcg aaccgccaga cggaacaaaa aatcaagaaa gaatacaggt 5581 atctgtaact cttgatcctc tatggttaaa tacagaacaa agcgcaaaaa gaaatcaaaa 5641 ggtaaaaata aatggcgaag ttaagcgtgt tgggaaaagt gaagttcaat gaaacattct 5701 ttttcccttt aaaaaaagag tggctttatt atatccaaga caatgatgct ttattagaaa 5761 agatagacgc aattgctact gaagaaaatg gggaaattgg gattaagttt ttaaaacgat 5821 acggaattaa tcctaaagaa aatgaatccg ttaaagaata cttagaggca cgagaaaaag 5881 ctgataaagc ttatcttgaa aaaattaaag caattgggca aaaaacggga ctatccacta 5941 ctgagattga gggggtagta gttaacgacg gctcgatccg agaacggatc gagcaggtta 6001 tggtcgatgc ccttgacggg gtaaaatctg acagtgtaga gcaaaaagta gaaaccgccg 6061 ctattgtaca gcaatcgata ttaaacaacc gtaagaaaac aagagaattg acacgagaat 6121 cgatagaact tgtggaacct tatctcgatg aactaaacag cctgttcaag gatcgggaaa 6181 cgacctacga aacttacaat aaagccttat tagctaattt tcttggtagt cctcgccggg 6241 tagttaaact caaagataag tcagctattg agtttaccat agaagacatt aacgatatgt 6301 ctcaatttat ggtagtaaaa ctctatcaag actatctctg gcaggatata acccagtggc 6361 aaaacccaga acctgaaaaa ccagaacctg aaaaaacaga gtcagagcca acggaggatg 6421 acgaaaaaaa cgaatagagg acgcaattaa tgcgcggtta gaagcaatcg ctaaccccat 6481 taattgggaa gaaatctatt acaaatggtg tgcatgggga ctacccattg aagaatggga 6541 agagtggcca gactggttaa tcctaaaaaa atattcaggg attcaaaaag tcaaagcaga 6601 agagattaat tcactatcgg gtacagttag tcagattgcc gccatggttc acgcctacct 6661 gatagcacaa tccaaagaag gttctaagtc acaaaatctt aatcccagtg attttctgcc 6721 ttaccagttc aaagaaaata aaaaatattt tcttgatcga gaaaccgctc aaattctgtt 6781 agaagctatg aaagttgggc gagtgccagt cttcgccact cagataatag tcgattgcgg 6841 actatacgac gaaataattc aattaatagg ggagaaaagc taatgtcttt atcacttggt 6901 actttagaaa tcggtctagg gttaaataca gcccaatatg atagcggtat caaatcggct 6961 aaagacaaac tttcttcctt agaggatcat gtccaaaaaa tcactagaga catggaatgg 7021 tctcttaaac aaagtattcc taagctgaca attgttccgt cagtagatca ccgtccatta 7081 catggtttaa ataaacattt atcagaaaaa gaaaaacaca ttgatcgggt tagcgataaa 7141 acaatagctc catcagttga tctaagtaaa ttagatgatt tttttgaaaa gctagaatca 7201 attcaagatt tgctatcagg ttcactagaa atatctcttg attctagtgg atttgaaagc 7261 tcccttgatt caatggctga gtcagcacaa aacagcttac aagaaaacct agaagcattt 7321 aaacagaata ctgccaattt tgcaaaattt gctacggctc cttttgaagc tgtttttacg 7381 ggtgtttttg aaggaattgg gagcgttata tctaaaaaaa ttggcaaagg attagaatct 7441 tctttaaaaa aatctacaaa tatttcactt gaaggaatag gaggattttt aggagatgta 7501 actagcgaag tattatctca aatcgacgaa acggcgggac taagtttaaa cgttagaaac 7561 gttaaaacta ctgataaaaa tcaaaaaaac acaaaaagaa tatcaagtgc agaaacagaa 7621 gacggaacta aacctaaaaa actctctaaa gttgaacagt ctgatgcgat tgccaaaagc 7681 attcaaaaaa cagcgcaagt tatcgacaaa ataactgata atgcagttaa atttgccaat 7741 actgtagaga acatagagtc ggttattaat ttggtgtctt cagtgttatc aaatattgct 7801 aatgacactc agtcttttaa acctgatctt actaatatca aaaatatttc tagattagag 7861 ccagttcctg ttttagccga aagaagccct agtcctaaat ccaacgcatt aaatgcgggc 7921 aaaatagcgt cagaacaagc tattagtaca ccaagagcta cacaacaaaa tgcgcctaag 7981 ttagaaatag ctgggaacta taaaaattta gagcaaaata ctgatgcttt tattgaagaa 8041 acgatgtcgc acgttcggca tcaatcaaaa atgtatcaaa gtgtagcgca ggcacttaat 8101 aaaacaggcg ataaaagaga aagattaaaa actttactta ataaagcaga aaaagggaaa 8161 agagaattaa accctaaaga attggcagaa gcgttagaaa taataaaaga aataagagga 8221 aaagctgaat ttttattaaa tgatgatata gcaaaacgag cagaaggaag aatcaacgat 8281 ttaggcaaaa atctaccaga aaaagataaa aaatttggaa cagaatttca aagaattcaa 8341 gccgatattg ctaatcggaa aaatacagca cgaaaagcta ttagtgctac agaagctttt 8401 gttagcgcac caaaaacaga tgtagaaaaa caaattttta aagctatttc ttctggacaa 8461 gcatctcaaa attgggacaa aacgactgca tcaatcaaag aaaatattaa tgagattgta 8521 caagagtcac aaagaaattc taaaaaaata caaggaggta tatcagaagc gtcacctggt 8581 cctacattta atgctcgtaa aaactggaaa aaaaccagtg attacattaa agaacaaatt 8641 aaattaattg ttgaagagtc taaaaacgca tcaaaacaga ttgacggtaa ctttgctaat 8701 cctgacttaa aacgacaatt gcgattagag cgagttattg aatctaagtt aaaacaaggg 8761 caaagcatac ctagtccagt tttaagtgaa ttaaaaggaa tttatactcg tccaaattta 8821 acttcgccac aaattaatga cgattattta aataaattgc aaaaagctgt tcaaattgct 8881 atcagacaat ttggagttaa cagtgataga gctattcaat tattagaaaa gttagagtct 8941 gctagaaaga ttgcggttaa gcaagaaacc gataaactta atcgtattcc taaaactaag 9001 caagaaataa acgaacgagc cgaactaact aaaaaaagaa aagaacaaga aaaaagacaa 9061 caacaaaaga tagaaaaaga aatccaaaga gaggttaata gtctaaatag attagctaca 9121 tctctgtcta tcgctcaatc ccctgaaaga ctagctgaat tagaaagaaa acgtcaagct 9181 ataaaagata aatacgagca gagaagagcc aaagagtttc aagattggtt aaagactcag 9241 aatgtcggta tcaaccgtag taccaactgg gatagacagt ctagaattag tgagttaaga 9301 caatctattg gtcagcgtag agataatact aatcagttaa ttcaaaggga aattgatagc 9361 cttaatcgta tatctcaacc ttttgctagt agagatataa ccgaagatta tttaaataaa 9421 ttacagaaag ccgtccaaat tgctatcaga caatttggag ctaatagcag tagggctatt 9481 cagttactag atagattaaa tactactaga aatgtctttg ctaaacagga aatcgataaa 9541 attaaccgtg tccctaaaac taagcaagaa ataaatgagc aagctgaatt aactaaaaaa 9601 agaaaagaac aagacaaaaa ccagcaacaa aaagcagaga aaaaagtcca aaaagaaatt 9661 actgacttaa acagattggc atcatcttta tctatcgctc aatcccctga aagattagct 9721 gaattagaaa gaaaacgtca agcgataaaa aataagtatg aagaaaaaag agccaaagag 9781 ttccaagatt ggatgaaagg acaaaaaaca attggtatta atcgtaacac caattggaat 9841 agacaatcta gaattaacga actaagacag tctattagtc aacgtagaga taatactaat 9901 caattaattc aaaaagaggt tgatggatta aatagattag catcttctca atcccctgaa 9961 agattagctg aattagaaag aaaacgtcaa gcgataaaaa ataagtatga agaaaaaaga 10021 gctaaagagt ttcaagattg gttaaagact cagaatgtcg gtatcaaccg tagtaccaat 10081 tggaatagac agtctcgtat tagcgagtta agacaatcta ttggtcagcg tagagataat 10141 actaatcagt taattcaaag ggaagttgat ggattaaata gattggcttc gccttttagc 10201 aacagaaata taactgatag ttatttaaat aaattacaga aagccgtcca aattgctatc 10261 agacaatttg gaaccaatag taatagaacc attgaattgt taaacaaatt agaatctgct 10321 agagaaattg tagctaaaca ggaagctgat aaagttaatc gtgtccctaa aactaagcaa 10381 gaaataaacc aacaagcaga atctattaaa agaaaaagag agcaagaagc taaattaaaa 10441 caagaaacag aaagaagtgt acagaaagag attactgatt taaacagatt agccacatct 10501 ttatctatcg ctcaatctcc tgagagacta ggcgatattg aggctaagcg tcaggctata 10561 aaagacaggt acgaacagaa acgagctaaa gagtttcaag attggttaaa aactcaaaag 10621 acaattggca tcaaccgtag taccaactgg gatagacagt ctagaattag tgagttaaga 10681 caatctattg gtcagcgtag agataatact aaccagttga ttcaaaggga agttgatggg 10741 cttaaccgca tatcttcacc ttctttcgct actaaagaga ttaatcaaga ctatctaaac 10801 aaattgcaaa aagctgttca aattgctgtt agacaatttg gagctaatag caacagaact 10861 attgaactgt tgaacaaatt agaatctgct agagatgtct ttattcaaca agaagctaac 10921 aaagctcaaa agaatcaaag aattaaatcg cctcaagaat ggaaatctat taaagaacaa 10981 gagaaaaaac gacaacaaga agcagaaaaa agggtacaag aagaaataga aggattgcgt 11041 agattaggta ggccagaccc taaagcaatg gctaatatag aagccagaag aaaaattatt 11101 cttgacagac gtgctgctag agaaaaagaa gcgcgagaac gcgctgaatt ggcaagatta 11161 aaacaagaaa gacaagaaga aacatcttta cagggatttt taaatcgacc tctaagggca 11221 ttaaatcgag tccgtcgttc tacttttgct gatgcaaaaa tagaaacctt agctcaaaaa 11281 tatgcagatt ttggtactga atcttacttt gataagtttt tccgcaatat gagtaatcgt 11341 attaagcgaa aaattagcaa agaccctagc gaaagagaaa ccgtggcaag agaaggtttt 11401 ggtatgcttt ttggcatgac tgcaatgtct aatcctttat tgccagcatt agcatcttta 11461 gctcctttag ccttgccatt actaccgacg gctttagctg tatctggtgg ggttaacatg 11521 gtatcacctg cttttcaggg agctagtcag agaatacagg gagtagaaaa ctttaatgct 11581 agattgaacg ctgttacagg cgatccgctt aaatctgaca ctgaatacaa gtatctgtta 11641 ggattagcag aaaaatatcg agtttccttg cagtctttgt cagaaggata tactcaatta 11701 gcagtagcaa caaggggaac aaaactagaa ggtgaagcga ctaaagagct tttttctgct 11761 attacagcgt caatcaaagc cttgcgatta aatacctttg acgctggttt agttcttaac 11821 gcctacactc agattgttgc taagggtaaa atatcaatgg aagaactccg gcaacagtta 11881 ggggaaaagt ttccccctac tatgtcagta tttgctaaag cactaggagt tagccaatca 11941 gaactaggtc aattagtggc aaaaggttcg cttttatccg atgaagtttt accaaaagtc 12001 acaaaaattc tagagcaaga attcggagcc gcagcagaaa aaggtacaag cgattttacc 12061 agtgcgcttg cctcaatgga gacagcttta tttaaattag atcggaattt tactgacatt 12121 tttgggggta tgtttgctgg gattaccaat ctagcatctg gtattgttac atcttttagt 12181 gataacttcc gaattatctc acaactcgca ggagcattta cgattggtgt agtagcgcaa 12241 gttggcattg ggctgtcaac cattttagca atgcctaaaa tagcacctgt tgttagtaat 12301 atacaagggt ttataatggg cgcgtggaaa acaggcttaa ttaccttaac cccatttatg 12361 tctggtattt tagccgatgt aatcgatgac gtttttaata ctgaaaacga cctgtttaca 12421 aacatggcga aaggaactac aaacataatc gccgcttcta ttggtagttt agagaatatt 12481 tcttacggtg catcaggaaa aggctttttt ggttacaact gggaaaaagc agtcaatcca 12541 atacaatcaa tcggaaatac attaggtaat ctttttaaat ctattcctag cggtgttttt 12601 gagctaactg ctttaacttt tatgtttgaa cagcttattg tattagggca aatgttctta 12661 gtacctttac tgagtaaact atggttaact tttaaaggaa tgagtggggc gatatggaca 12721 gcggctacta atgctaaaag tttaggggga gtatttgata ctttatttgc cagtagtgct 12781 atggctaaag gggctttagt tgctcttaat atcgctctta aggcttttgt tgctctagga 12841 tttatggctt ttgctagaag tgattttaca aatcctttac aggccagttt aaacgaatca 12901 gaacaaaaaa taggtgcaag cattgacaat attcaaaaat tattaaataa gttagaatct 12961 cctacattag aagtaaaagt taaaggagga gatggactcc tctcaaaagg gttagaacta 13021 aacttaatgg ctatgctagg ggacgaaagt tctagctaca aaacagatga tgcaattaaa 13081 gaagggaaag gatttattcc tagtgataat ccattaatcc gatttcatgc agagcatcta 13141 gcaaaacaag aagttcccgt aaaacaacaa gaagctaaaa gatttggctt agaaaaatat 13201 tttaatgact ataatgggtt tttcacgctt gcacaagatc agcttttaaa tagtgctaga 13261 gacttaagta gagttaacca agaggtatca aaggcgttaa ccaaacagtc gttaatgcct 13321 gaaaccgtag gtaaatcgat caatttactt aatccctttg ctaatgaagc taaaataatt 13381 gatgcttcta tacaaagact acaaaaagaa agaacaaccg cggggctaac aaatacatct 13441 gaaagtaaag ttagaatcag agaaatagac aaagaaatta aaagacttaa tgatcttaga 13501 aaagaaaaga ttaagccttt agacgaattt atgactgatt ttgagtcatt aagaaaagga 13561 ttaaacagtc gtatcgaagc gattgataaa caggatttac cagaaggagc taaaaaggct 13621 ttaagggcaa ttgtagaagg caatcaaaaa atagtcgaag aaaccagaaa atccttagaa 13681 gaagccggtt tttatgacat actaactcca ttagaatcgg catggcaaaa agcgactgat 13741 gcaataagag attttgaagt tcaatacgaa aaaacaatgc gacgtattga aaataaatct 13801 ttaaatgatc aaacagcagt ttatcgttct aacttaaatc cagtgcaaat tggagaacaa 13861 accagaaata ttgaaattca aactcaaaaa gaacgaatta aacaatttga ggcaaaccta 13921 acaaaacaac agcaaaccct agaacaatat ttaagcgttc cagaatcagg gcgaactcaa 13981 gagcaggtag atgcgattga ggaaacgaga aaagctacat ttgaagcgga gaaagaacta 14041 gcatcagcta gattatctct tgctaagtct gaatatgacg ctactcaaga tagactaaga 14101 aaacagcagt cagaaactaa attaagagta gattctgaaa ttgcaagaaa acgtattgat 14161 gtaattcgtg ctaatccgtt tggtggtgtt aatgcttctt taagaaatgc tgaaatagat 14221 gtagaagaaa ggattcaagc cattggatta ctttatcagc aactagcaga taatactggt 14281 aatccaatag aaattaaatc acaaatcgtt gcagcagaat tagccctaga acaagcccgc 14341 gctaacctat tacagcaaca aacctcgcta caagactatt accgcaacct tgaccgtcag 14401 atagttgact ttaaccggca gattgaggac tacagaagac aaatcgaaga tgctcaacta 14461 tcagcattta aagagaatcg ttccctatct gaaagttaca gtgatttagt cagggaactc 14521 gataagaacc tcttaaatgc ccaaaatcag ttactagata caaccgatag aatcagggta 14581 caacaagtta aaaatcgttt attgataccc ggtacaagcg acgctggcag agaattaggt 14641 gacattttcc tagaatttgt acagggacaa gctgacattg ccagtcgcgg tcgtaccttc 14701 cagtcccgaa ctgaagagat agagacttct tatatctcta ccttaagaaa tattcgcaac 14761 ttacaagagc aacagcaaga ggctgaaaga aaccggctaa gaacaattga ggatattaaa 14821 cggactcaag agaaccttaa tcgcaccttg gctgatttaa tccgacaaac caataaagaa 14881 ttaggtttta ttccccaatc aatcaaggat attgtcacaa atcttaatac cttaccagag 14941 ccgattaaat taatcaattc tgagttagta gccattcccc caaatatcaa gacttctggg 15001 gaagatttag taaaaagtat agaggaaact gcggaagcaa tcagaaaagc taaggaaggt 15061 ttaatactac cggctccggg taattttat //